AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780037139.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780037139.01.T01 MTR_5g093010 76.433 157 25 1 1 157 1 145 6.41e-86 248
MsG0780037139.01.T01 MTR_7g028905 95.690 116 5 0 1 116 1 116 2.50e-82 238
MsG0780037139.01.T01 MTR_2g068760 50.476 105 52 0 6 110 14 118 1.20e-38 129
MsG0780037139.01.T01 MTR_5g083230 51.376 109 53 0 6 114 7 115 7.16e-38 127
MsG0780037139.01.T01 MTR_5g083960 46.970 132 70 0 6 137 40 171 1.16e-37 127
MsG0780037139.01.T01 MTR_4g083680 50.943 106 52 0 6 111 16 121 8.95e-37 125
MsG0780037139.01.T01 MTR_3g031660 48.148 108 56 0 6 113 8 115 2.98e-36 123
MsG0780037139.01.T01 MTR_3g094690 52.830 106 50 0 6 111 48 153 3.17e-36 125
MsG0780037139.01.T01 MTR_4g105170 51.402 107 52 0 6 112 12 118 1.29e-35 122
MsG0780037139.01.T01 MTR_3g071590 53.000 100 47 0 6 105 9 108 1.47e-35 121
MsG0780037139.01.T01 MTR_7g033800 46.774 124 57 1 6 120 12 135 5.58e-35 120
MsG0780037139.01.T01 MTR_3g077240 50.926 108 50 1 6 110 11 118 1.30e-34 120
MsG0780037139.01.T01 MTR_4g060950 46.429 112 59 1 1 111 20 131 2.89e-34 119
MsG0780037139.01.T01 MTR_3g094690 52.830 106 49 1 6 111 48 152 4.29e-34 119
MsG0780037139.01.T01 MTR_3g452660 49.550 111 56 0 6 116 8 118 5.35e-34 121
MsG0780037139.01.T01 MTR_3g073690 47.573 103 54 0 6 108 10 112 8.56e-34 118
MsG0780037139.01.T01 MTR_2g093310 44.144 111 62 0 1 111 29 139 1.19e-33 117
MsG0780037139.01.T01 MTR_6g005070 43.077 130 74 0 6 135 10 139 7.62e-33 114
MsG0780037139.01.T01 MTR_5g075020 45.865 133 68 1 6 138 44 172 1.90e-32 115
MsG0780037139.01.T01 MTR_5g017950 48.544 103 53 0 6 108 9 111 2.41e-32 117
MsG0780037139.01.T01 MTR_5g075020 45.865 133 68 1 6 138 48 176 2.43e-32 115
MsG0780037139.01.T01 MTR_1g095850 40.845 142 76 1 6 147 73 206 2.73e-32 115
MsG0780037139.01.T01 MTR_8g040900 50.467 107 51 1 6 112 8 112 4.67e-32 114
MsG0780037139.01.T01 MTR_6g005080 48.000 100 52 0 6 105 11 110 1.01e-30 108
MsG0780037139.01.T01 MTR_3g071420 46.392 97 52 0 6 102 15 111 5.57e-30 107
MsG0780037139.01.T01 MTR_5g083010 44.554 101 56 0 6 106 12 112 9.95e-30 107
MsG0780037139.01.T01 MTR_7g096530 46.154 104 55 1 6 108 15 118 1.01e-29 108
MsG0780037139.01.T01 MTR_6g011250 36.111 144 91 1 4 146 8 151 1.83e-28 103
MsG0780037139.01.T01 MTR_1g070220 48.000 100 51 1 6 104 16 115 4.46e-28 103
MsG0780037139.01.T01 MTR_6g027710 43.396 106 59 1 6 110 41 146 7.25e-28 103
MsG0780037139.01.T01 MTR_8g079660 40.952 105 62 0 2 106 5 109 1.18e-26 101
MsG0780037139.01.T01 MTR_7g096610 39.007 141 83 2 6 143 9 149 5.20e-26 98.6
MsG0780037139.01.T01 MTR_6g011230 41.414 99 58 0 4 102 10 108 7.12e-26 97.8
MsG0780037139.01.T01 MTR_8g036085 41.509 106 61 1 6 110 24 129 1.30e-25 97.8
MsG0780037139.01.T01 MTR_1g070205 44.118 102 56 1 6 106 13 114 2.31e-25 97.4
MsG0780037139.01.T01 MTR_7g074990 44.554 101 55 1 6 105 21 121 3.04e-25 97.1
MsG0780037139.01.T01 MTR_8g079580 43.810 105 58 1 2 105 5 109 2.26e-24 94.7
MsG0780037139.01.T01 MTR_6g011200 37.273 110 68 1 4 112 8 117 3.12e-24 94.7
MsG0780037139.01.T01 MTR_5g080470 41.176 102 59 1 6 106 8 109 2.25e-23 91.3
MsG0780037139.01.T01 MTR_8g079620 37.143 105 66 0 2 106 5 109 4.59e-23 91.3
MsG0780037139.01.T01 MTR_6g027700 37.405 131 69 3 6 123 8 138 4.86e-23 90.5
MsG0780037139.01.T01 MTR_8g036105 41.176 102 59 1 6 106 19 120 5.76e-23 90.5
MsG0780037139.01.T01 MTR_4g099230 37.037 108 67 1 6 112 3 110 1.27e-22 88.2
MsG0780037139.01.T01 MTR_8g017090 39.604 101 61 0 6 106 13 113 7.22e-22 89.4
MsG0780037139.01.T01 MTR_4g107450 42.000 100 58 0 6 105 11 110 1.16e-21 89.0
MsG0780037139.01.T01 MTR_5g085390 36.283 113 70 2 4 114 8 120 7.61e-21 85.5
MsG0780037139.01.T01 MTR_7g075230 39.423 104 60 2 6 106 13 116 7.62e-21 84.7
MsG0780037139.01.T01 MTR_2g100020 34.653 101 66 0 6 106 20 120 4.29e-19 81.6
MsG0780037139.01.T01 MTR_4g088035 33.721 86 57 0 6 91 7 92 7.74e-14 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780037139.01.T01 AT3G26660 66.990 103 34 0 4 106 4 106 1.46e-48 153
MsG0780037139.01.T01 AT3G26620 66.990 103 34 0 4 106 4 106 1.85e-48 152
MsG0780037139.01.T01 AT2G30130 52.778 108 51 0 6 113 9 116 8.45e-39 130
MsG0780037139.01.T01 AT1G31320 53.097 113 52 1 6 117 14 126 2.51e-36 124
MsG0780037139.01.T01 AT2G30340 50.943 106 52 0 6 111 53 158 3.34e-35 123
MsG0780037139.01.T01 AT2G30340 50.943 106 52 0 6 111 54 159 3.60e-35 123
MsG0780037139.01.T01 AT1G07900 43.902 123 64 1 6 128 34 151 5.23e-35 120
MsG0780037139.01.T01 AT5G63090 46.957 115 61 0 6 120 12 126 1.10e-34 120
MsG0780037139.01.T01 AT5G63090 46.957 115 61 0 6 120 12 126 1.10e-34 120
MsG0780037139.01.T01 AT5G63090 46.957 115 61 0 6 120 12 126 1.10e-34 120
MsG0780037139.01.T01 AT5G63090 46.957 115 61 0 6 120 12 126 1.10e-34 120
MsG0780037139.01.T01 AT2G28500 45.946 111 60 0 1 111 51 161 2.54e-34 120
MsG0780037139.01.T01 AT3G27650 50.000 100 50 0 6 105 40 139 1.56e-33 116
MsG0780037139.01.T01 AT5G66870 48.515 101 52 0 6 106 8 108 8.92e-33 118
MsG0780037139.01.T01 AT2G40470 52.885 104 49 0 6 109 21 124 1.25e-32 115
MsG0780037139.01.T01 AT2G40470 52.885 104 49 0 6 109 46 149 2.04e-32 115
MsG0780037139.01.T01 AT1G16530 50.485 103 50 1 6 107 15 117 4.42e-32 112
MsG0780037139.01.T01 AT1G65620 48.077 104 54 0 6 109 10 113 5.78e-32 113
MsG0780037139.01.T01 AT1G65620 48.077 104 54 0 6 109 10 113 5.78e-32 113
MsG0780037139.01.T01 AT1G65620 48.077 104 54 0 6 109 10 113 5.78e-32 113
MsG0780037139.01.T01 AT1G65620 48.077 104 54 0 6 109 10 113 5.78e-32 113
MsG0780037139.01.T01 AT1G65620 48.077 104 54 0 6 109 10 113 5.78e-32 113
MsG0780037139.01.T01 AT2G23660 48.515 101 52 0 6 106 6 106 5.91e-32 116
MsG0780037139.01.T01 AT2G23660 48.515 101 52 0 6 106 6 106 5.91e-32 116
MsG0780037139.01.T01 AT2G23660 48.515 101 52 0 6 106 6 106 5.91e-32 116
MsG0780037139.01.T01 AT3G11090 44.660 103 57 0 6 108 12 114 2.58e-31 110
MsG0780037139.01.T01 AT4G00210 37.500 144 80 3 6 139 12 155 2.10e-27 102
MsG0780037139.01.T01 AT2G42430 44.762 105 57 1 6 109 16 120 6.47e-27 101
MsG0780037139.01.T01 AT3G50510 34.641 153 84 2 2 154 9 145 6.62e-27 100
MsG0780037139.01.T01 AT3G50510 34.641 153 84 2 2 154 9 145 6.62e-27 100
MsG0780037139.01.T01 AT3G03760 44.554 101 55 1 6 105 52 152 7.72e-27 102
MsG0780037139.01.T01 AT2G42440 45.000 100 54 1 6 104 8 107 1.56e-25 98.2
MsG0780037139.01.T01 AT3G58190 44.118 102 56 1 6 106 12 113 3.37e-25 96.7
MsG0780037139.01.T01 AT5G06080 43.119 109 61 1 6 113 8 116 4.84e-25 95.1
MsG0780037139.01.T01 AT3G13850 35.714 154 91 2 6 151 37 190 5.13e-25 97.1
MsG0780037139.01.T01 AT4G00220 42.308 104 59 1 8 110 20 123 4.57e-24 94.0
MsG0780037139.01.T01 AT2G45420 42.308 104 59 1 8 110 40 143 1.56e-23 93.2
MsG0780037139.01.T01 AT2G45410 42.000 100 57 1 8 106 19 118 2.32e-23 91.3
MsG0780037139.01.T01 AT5G35900 37.624 101 63 0 6 106 6 106 6.34e-22 87.8
MsG0780037139.01.T01 AT3G47870 37.500 104 65 0 6 109 37 140 9.44e-22 89.7
MsG0780037139.01.T01 AT2G31310 34.314 102 66 1 6 106 8 109 4.15e-20 82.4
MsG0780037139.01.T01 AT1G06280 37.931 87 54 0 6 92 25 111 5.04e-18 77.4
MsG0780037139.01.T01 AT1G72980 36.000 100 64 0 6 105 14 113 8.15e-18 77.0
MsG0780037139.01.T01 AT2G19820 34.043 94 61 1 6 98 13 106 1.72e-15 68.9
MsG0780037139.01.T01 AT4G22700 33.043 115 71 3 1 110 1 114 2.06e-13 65.1
MsG0780037139.01.T01 AT5G15060 43.243 74 38 2 6 76 18 90 9.81e-13 62.0
MsG0780037139.01.T01 AT1G36000 30.682 88 61 0 4 91 8 95 2.50e-12 60.5

Find 31 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCTTCAAATTGTTGATTT+TGG 0.080390 7:-20519368 None:intergenic
AGGATTCAAGATCCTGTTTA+TGG 0.232394 7:+20519252 MsG0780037139.01.T01:CDS
GGCCTAGTCAAACTCCTAAT+TGG 0.295938 7:+20519481 MsG0780037139.01.T01:CDS
GGATCATTGGGAGGGAAATA+TGG 0.321654 7:-20518722 None:intergenic
GCTTCTGTTCACCGAATCTA+TGG 0.327300 7:+20518755 MsG0780037139.01.T01:CDS
AGCAAATCTTTGAGGATCAT+TGG 0.352548 7:-20518735 None:intergenic
AACTCCTAATTGGTTCAATT+AGG 0.365498 7:+20519491 MsG0780037139.01.T01:CDS
TTAACATCATGATTTATAGA+AGG 0.383363 7:+20518645 None:intergenic
AAGTCCTAATTGAACCAATT+AGG 0.385923 7:-20519495 None:intergenic
ATCCCAACACATCCATAAAC+AGG 0.432009 7:-20519264 None:intergenic
GCAAATCTTTGAGGATCATT+GGG 0.442273 7:-20518734 None:intergenic
CTCCAAAATCAACAATTTGA+AGG 0.458414 7:+20519366 MsG0780037139.01.T01:CDS
ATCTATGGTGGTAGCAATGT+TGG 0.480363 7:+20518770 MsG0780037139.01.T01:CDS
TGTATTTAGAAGCACAATGT+AGG 0.497154 7:+20519232 MsG0780037139.01.T01:CDS
ATCCTGTTTATGGATGTGTT+GGG 0.518295 7:+20519262 MsG0780037139.01.T01:CDS
TCAAGATACAGAAGTTGAGT+TGG 0.518830 7:+20519311 MsG0780037139.01.T01:CDS
TATGGAGAGAAAATGCAATC+AGG 0.521384 7:-20518704 None:intergenic
ACAACATGGAGCAGATTCAA+TGG 0.528352 7:+20519460 MsG0780037139.01.T01:CDS
CATTGCTACCACCATAGATT+CGG 0.528587 7:-20518766 None:intergenic
GATCCTGTTTATGGATGTGT+TGG 0.530554 7:+20519261 MsG0780037139.01.T01:CDS
TGAACAGAAGCAAATCTTTG+AGG 0.534397 7:-20518743 None:intergenic
AATACATAGAATTTGCAGCT+TGG 0.550687 7:-20519215 None:intergenic
CTTGCAAGAGTCAAAGAAGA+AGG 0.562843 7:+20518675 MsG0780037139.01.T01:CDS
ATCTTTGAGGATCATTGGGA+GGG 0.584186 7:-20518730 None:intergenic
AATCTTTGAGGATCATTGGG+AGG 0.604113 7:-20518731 None:intergenic
TCTGTTCACCGAATCTATGG+TGG 0.619615 7:+20518758 MsG0780037139.01.T01:CDS
AACCAATTAGGAGTTTGACT+AGG 0.647531 7:-20519483 None:intergenic
TCCATGTTGTTACTATGTAG+TGG 0.647940 7:-20519447 None:intergenic
GCCACTACATAGTAACAACA+TGG 0.651155 7:+20519446 MsG0780037139.01.T01:CDS
GCTTGGTGTCGTACATAGTG+AGG 0.654586 7:-20519198 None:intergenic
GGAGAGAAAATGCAATCAGG+AGG 0.655607 7:-20518701 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTAAAATAATCTTATTG+TGG + Chr7:20519153-20519172 MsG0780037139.01.T01:intron 10.0%
!! AACATTATATAATTATAGCA+TGG + Chr7:20519062-20519081 MsG0780037139.01.T01:intron 15.0%
!!! TAGTATCTTTTTTTTTTTGA+AGG + Chr7:20518874-20518893 MsG0780037139.01.T01:intron 15.0%
!! TACCATGTAATATATTATTG+TGG + Chr7:20518918-20518937 MsG0780037139.01.T01:intron 20.0%
!! TCAATGTAAGAATCAATATT+TGG + Chr7:20518961-20518980 MsG0780037139.01.T01:intron 20.0%
!! TTCCACAATAATATATTACA+TGG - Chr7:20518923-20518942 None:intergenic 20.0%
AATACATAGAATTTGCAGCT+TGG - Chr7:20519218-20519237 None:intergenic 30.0%
CTCCAAAATCAACAATTTGA+AGG + Chr7:20519366-20519385 MsG0780037139.01.T01:CDS 30.0%
! TGTATTTAGAAGCACAATGT+AGG + Chr7:20519232-20519251 MsG0780037139.01.T01:CDS 30.0%
!!! CACCTTCAAATTGTTGATTT+TGG - Chr7:20519371-20519390 None:intergenic 30.0%
AACCAATTAGGAGTTTGACT+AGG - Chr7:20519486-20519505 None:intergenic 35.0%
AGGATTCAAGATCCTGTTTA+TGG + Chr7:20519252-20519271 MsG0780037139.01.T01:CDS 35.0%
ATCCTGTTTATGGATGTGTT+GGG + Chr7:20519262-20519281 MsG0780037139.01.T01:CDS 35.0%
CAATGTTGGAAAAATGCTTC+AGG + Chr7:20518784-20518803 MsG0780037139.01.T01:CDS 35.0%
TATGGAGAGAAAATGCAATC+AGG - Chr7:20518707-20518726 None:intergenic 35.0%
TCAAGATACAGAAGTTGAGT+TGG + Chr7:20519311-20519330 MsG0780037139.01.T01:CDS 35.0%
TCCATGTTGTTACTATGTAG+TGG - Chr7:20519450-20519469 None:intergenic 35.0%
TGAACAGAAGCAAATCTTTG+AGG - Chr7:20518746-20518765 None:intergenic 35.0%
! AGCAAATCTTTGAGGATCAT+TGG - Chr7:20518738-20518757 None:intergenic 35.0%
! GCAAATCTTTGAGGATCATT+GGG - Chr7:20518737-20518756 None:intergenic 35.0%
ACAACATGGAGCAGATTCAA+TGG + Chr7:20519460-20519479 MsG0780037139.01.T01:CDS 40.0%
ATCCCAACACATCCATAAAC+AGG - Chr7:20519267-20519286 None:intergenic 40.0%
ATCTATGGTGGTAGCAATGT+TGG + Chr7:20518770-20518789 MsG0780037139.01.T01:CDS 40.0%
CATTGCTACCACCATAGATT+CGG - Chr7:20518769-20518788 None:intergenic 40.0%
CTTGCAAGAGTCAAAGAAGA+AGG + Chr7:20518675-20518694 MsG0780037139.01.T01:CDS 40.0%
GATCCTGTTTATGGATGTGT+TGG + Chr7:20519261-20519280 MsG0780037139.01.T01:CDS 40.0%
GCCACTACATAGTAACAACA+TGG + Chr7:20519446-20519465 MsG0780037139.01.T01:CDS 40.0%
! AATCTTTGAGGATCATTGGG+AGG - Chr7:20518734-20518753 None:intergenic 40.0%
! ATCTTTGAGGATCATTGGGA+GGG - Chr7:20518733-20518752 None:intergenic 40.0%
GCTTCTGTTCACCGAATCTA+TGG + Chr7:20518755-20518774 MsG0780037139.01.T01:CDS 45.0%
GGAGAGAAAATGCAATCAGG+AGG - Chr7:20518704-20518723 None:intergenic 45.0%
GGATCATTGGGAGGGAAATA+TGG - Chr7:20518725-20518744 None:intergenic 45.0%
GGCCTAGTCAAACTCCTAAT+TGG + Chr7:20519481-20519500 MsG0780037139.01.T01:CDS 45.0%
TCTGTTCACCGAATCTATGG+TGG + Chr7:20518758-20518777 MsG0780037139.01.T01:CDS 45.0%
!! GCTTGGTGTCGTACATAGTG+AGG - Chr7:20519201-20519220 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr7 gene 20518653 20519512 20518653 ID=MsG0780037139.01;Name=MsG0780037139.01
Chr7 mRNA 20518653 20519512 20518653 ID=MsG0780037139.01.T01;Parent=MsG0780037139.01;Name=MsG0780037139.01.T01;_AED=0.16;_eAED=0.16;_QI=0|0|0|1|1|1|2|0|157
Chr7 exon 20518653 20518805 20518653 ID=MsG0780037139.01.T01:exon:5030;Parent=MsG0780037139.01.T01
Chr7 exon 20519192 20519512 20519192 ID=MsG0780037139.01.T01:exon:5031;Parent=MsG0780037139.01.T01
Chr7 CDS 20518653 20518805 20518653 ID=MsG0780037139.01.T01:cds;Parent=MsG0780037139.01.T01
Chr7 CDS 20519192 20519512 20519192 ID=MsG0780037139.01.T01:cds;Parent=MsG0780037139.01.T01
Gene Sequence

>MsG0780037139.01.T01

ATGATTTATAGAAGGTGTGCTGCTTGCAAGAGTCAAAGAAGAAGGTGTCCTCCTGATTGCATTTTCTCTCCATATTTCCCTCCCAATGATCCTCAAAGATTTGCTTCTGTTCACCGAATCTATGGTGGTAGCAATGTTGGAAAAATGCTTCAGCAATTGCCTCACTATGTACGACACCAAGCTGCAAATTCTATGTATTTAGAAGCACAATGTAGGATTCAAGATCCTGTTTATGGATGTGTTGGGATTATCTCTAAATTATCTCAACAAATTCAAGATACAGAAGTTGAGTTGGCTAAAATCAAAACTCAGATTGCTTATCATAAACTCCAAAATCAACAATTTGAAGGTGAATCAAACTTCAATGTCTTGTCAACAATTGATGCTGAATCAAACTTAAGCTCTTTGCCACTACATAGTAACAACATGGAGCAGATTCAATGGCCTAGTCAAACTCCTAATTGGTTCAATTAG
Protein sequence

>MsG0780037139.01.T01

MIYRRCAACKSQRRRCPPDCIFSPYFPPNDPQRFASVHRIYGGSNVGKMLQQLPHYVRHQAANSMYLEAQCRIQDPVYGCVGIISKLSQQIQDTEVELAKIKTQIAYHKLQNQQFEGESNFNVLSTIDAESNLSSLPLHSNNMEQIQWPSQTPNWFN*