Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037262.01.T01 | RHN62886.1 | 82.353 | 51 | 9 | 0 | 2 | 52 | 48 | 98 | 4.80E-20 | 85.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037262.01.T01 | A0A396IB98 | 82.353 | 51 | 9 | 0 | 2 | 52 | 48 | 98 | 2.29e-20 | 85.9 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0780037262.01.T01 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037262.01.T01 | MTR_3g005220 | 66.000 | 50 | 14 | 1 | 1 | 50 | 79 | 125 | 1.88e-17 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 11 sgRNAs with CRISPR-Local
Find 14 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAACAAATGGATTGATAAT+TGG | 0.321466 | 7:-22678196 | MsG0780037262.01.T01:CDS |
| ATAACTAAATCAAGAACAAA+TGG | 0.450621 | 7:-22678208 | MsG0780037262.01.T01:CDS |
| AAAGGTTGTAGCTTTCGCTT+GGG | 0.477046 | 7:-22678235 | MsG0780037262.01.T01:CDS |
| CAAAGGTTGTAGCTTTCGCT+TGG | 0.479905 | 7:-22678236 | MsG0780037262.01.T01:CDS |
| AAATGGATTGATAATTGGTC+AGG | 0.480078 | 7:-22678191 | MsG0780037262.01.T01:CDS |
| TCAGGAGATTCTTTGTTCAA+AGG | 0.531562 | 7:-22678173 | MsG0780037262.01.T01:CDS |
| AGATTCTTTGTTCAAAGGAG+GGG | 0.531979 | 7:-22678168 | MsG0780037262.01.T01:CDS |
| GAGATTCTTTGTTCAAAGGA+GGG | 0.547884 | 7:-22678169 | MsG0780037262.01.T01:CDS |
| GGAGATTCTTTGTTCAAAGG+AGG | 0.550163 | 7:-22678170 | MsG0780037262.01.T01:CDS |
| ATTACTATACGGGATATGAA+AGG | 0.604315 | 7:-22678261 | MsG0780037262.01.T01:CDS |
| ACGGGATATGAAAGGAACAA+AGG | 0.634481 | 7:-22678253 | MsG0780037262.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGATGCAAAAAATTACTATA+CGG | - | Chr7:22678167-22678186 | MsG0780037262.01.T01:CDS | 20.0% |
| !! | ATAACTAAATCAAGAACAAA+TGG | - | Chr7:22678231-22678250 | MsG0780037262.01.T01:CDS | 20.0% |
| ! | GATGCAAAAAATTACTATAC+GGG | - | Chr7:22678168-22678187 | MsG0780037262.01.T01:CDS | 25.0% |
| !!! | AGAACAAATGGATTGATAAT+TGG | - | Chr7:22678243-22678262 | MsG0780037262.01.T01:CDS | 25.0% |
| ATTACTATACGGGATATGAA+AGG | - | Chr7:22678178-22678197 | MsG0780037262.01.T01:CDS | 30.0% | |
| !! | AAATGGATTGATAATTGGTC+AGG | - | Chr7:22678248-22678267 | MsG0780037262.01.T01:CDS | 30.0% |
| !!! | GTAATTTTTTGCATCTTCGA+AGG | + | Chr7:22678163-22678182 | None:intergenic | 30.0% |
| AGATTCTTTGTTCAAAGGAG+GGG | - | Chr7:22678271-22678290 | MsG0780037262.01.T01:CDS | 35.0% | |
| GAGATTCTTTGTTCAAAGGA+GGG | - | Chr7:22678270-22678289 | MsG0780037262.01.T01:CDS | 35.0% | |
| TCAGGAGATTCTTTGTTCAA+AGG | - | Chr7:22678266-22678285 | MsG0780037262.01.T01:CDS | 35.0% | |
| AAAGGTTGTAGCTTTCGCTT+GGG | - | Chr7:22678204-22678223 | MsG0780037262.01.T01:CDS | 40.0% | |
| ACGGGATATGAAAGGAACAA+AGG | - | Chr7:22678186-22678205 | MsG0780037262.01.T01:CDS | 40.0% | |
| GGAGATTCTTTGTTCAAAGG+AGG | - | Chr7:22678269-22678288 | MsG0780037262.01.T01:CDS | 40.0% | |
| CAAAGGTTGTAGCTTTCGCT+TGG | - | Chr7:22678203-22678222 | MsG0780037262.01.T01:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 22678147 | 22678314 | 22678147 | ID=MsG0780037262.01;Name=MsG0780037262.01 |
| Chr7 | mRNA | 22678147 | 22678314 | 22678147 | ID=MsG0780037262.01.T01;Parent=MsG0780037262.01;Name=MsG0780037262.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|0|1|0|55 |
| Chr7 | exon | 22678147 | 22678314 | 22678147 | ID=MsG0780037262.01.T01:exon:3755;Parent=MsG0780037262.01.T01 |
| Chr7 | CDS | 22678147 | 22678314 | 22678147 | ID=MsG0780037262.01.T01:cds;Parent=MsG0780037262.01.T01 |
| Gene Sequence |
| Protein sequence |
