Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037345.01.T01 | XP_024626606.1 | 42.857 | 119 | 51 | 4 | 24 | 126 | 11 | 128 | 3.69E-18 | 84.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037345.01.T01 | Q9S7L2 | 46.774 | 62 | 32 | 1 | 15 | 76 | 260 | 320 | 1.87E-11 | 63.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037345.01.T01 | A0A072TYJ9 | 41.270 | 126 | 57 | 4 | 17 | 126 | 109 | 233 | 2.70e-17 | 84.3 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0780037345.01.T01 | TF | MYB-related |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037345.01.T01 | MTR_7g035300 | 41.270 | 126 | 57 | 4 | 17 | 126 | 109 | 233 | 6.86e-21 | 84.3 |
| MsG0780037345.01.T01 | MTR_7g037260 | 31.792 | 173 | 49 | 4 | 17 | 125 | 190 | 357 | 2.27e-17 | 76.6 |
| MsG0780037345.01.T01 | MTR_7g037130 | 31.214 | 173 | 50 | 4 | 17 | 125 | 190 | 357 | 1.13e-16 | 74.7 |
| MsG0780037345.01.T01 | MTR_3g463400 | 36.709 | 79 | 49 | 1 | 15 | 93 | 176 | 253 | 2.20e-13 | 65.9 |
| MsG0780037345.01.T01 | MTR_7g451170 | 46.667 | 60 | 31 | 1 | 17 | 76 | 236 | 294 | 1.01e-12 | 63.9 |
| MsG0780037345.01.T01 | MTR_5g070020 | 43.548 | 62 | 34 | 1 | 15 | 76 | 165 | 225 | 2.16e-12 | 62.8 |
| MsG0780037345.01.T01 | MTR_3g077650 | 45.161 | 62 | 33 | 1 | 15 | 76 | 224 | 284 | 8.19e-12 | 61.2 |
| MsG0780037345.01.T01 | MTR_1g057980 | 42.105 | 76 | 36 | 2 | 15 | 90 | 123 | 190 | 1.36e-11 | 60.1 |
| MsG0780037345.01.T01 | MTR_4g088015 | 46.667 | 60 | 31 | 1 | 17 | 76 | 266 | 324 | 2.61e-11 | 59.7 |
| MsG0780037345.01.T01 | MTR_4g105660 | 41.935 | 62 | 35 | 1 | 15 | 76 | 223 | 283 | 8.98e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037345.01.T01 | AT4G18770 | 46.774 | 62 | 32 | 1 | 15 | 76 | 260 | 320 | 1.90e-12 | 63.2 |
| MsG0780037345.01.T01 | AT5G58850 | 41.096 | 73 | 42 | 1 | 15 | 87 | 148 | 219 | 2.84e-12 | 62.4 |
| MsG0780037345.01.T01 | AT5G40360 | 43.243 | 74 | 37 | 2 | 15 | 84 | 201 | 273 | 4.14e-12 | 62.0 |
| MsG0780037345.01.T01 | AT5G11050 | 35.897 | 78 | 49 | 1 | 15 | 92 | 148 | 224 | 4.53e-11 | 59.3 |
| MsG0780037345.01.T01 | AT3G27785 | 38.710 | 62 | 37 | 1 | 15 | 76 | 232 | 292 | 6.10e-11 | 58.9 |
Find 17 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGTCGTTTAGGAACCTTTA+AGG | 0.215538 | 7:+24007385 | MsG0780037345.01.T01:CDS |
| CGTGTTCATTGAGTTGTTCT+TGG | 0.251185 | 7:-24007248 | None:intergenic |
| AAAATGTAGTTGAGAATAAT+TGG | 0.251203 | 7:+24007143 | MsG0780037345.01.T01:CDS |
| GAAATTGCTAAGAGATTTCC+AGG | 0.343232 | 7:+24007169 | MsG0780037345.01.T01:CDS |
| ATTTCATGAAGAAGTCGTTT+AGG | 0.390032 | 7:+24007374 | MsG0780037345.01.T01:CDS |
| TCATCACTAATATTAACATT+TGG | 0.410601 | 7:-24007289 | None:intergenic |
| GGAATGCCATTGAGCGTGTT+CGG | 0.431584 | 7:+24007221 | MsG0780037345.01.T01:CDS |
| TTCAGAGTGTGATTATGAAA+TGG | 0.434618 | 7:+24007315 | MsG0780037345.01.T01:CDS |
| GGCATTCCGAACACGCTCAA+TGG | 0.476690 | 7:-24007227 | None:intergenic |
| CACTAATATTAACATTTGGT+TGG | 0.486948 | 7:-24007285 | None:intergenic |
| AGAGTTCAGTTAAGAACCAT+TGG | 0.497650 | 7:+24007200 | MsG0780037345.01.T01:CDS |
| AAAGAGGAAACTATACTACT+TGG | 0.530182 | 7:+24007112 | MsG0780037345.01.T01:CDS |
| ACTGAACTCTCAGTACGGCC+TGG | 0.548161 | 7:-24007187 | None:intergenic |
| TATGCTTGCAGGACGAACCA+TGG | 0.592154 | 7:+24007083 | MsG0780037345.01.T01:CDS |
| CACGCTCAATGGCATTCCAA+TGG | 0.603862 | 7:-24007216 | None:intergenic |
| TCTTAACTGAACTCTCAGTA+CGG | 0.632743 | 7:-24007192 | None:intergenic |
| CAAGAACAACTCAATGAACA+CGG | 0.646667 | 7:+24007249 | MsG0780037345.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATGTAGTTGAGAATAAT+TGG | + | Chr7:24007143-24007162 | MsG0780037345.01.T01:CDS | 20.0% |
| !! | TCATCACTAATATTAACATT+TGG | - | Chr7:24007292-24007311 | None:intergenic | 20.0% |
| ! | CACTAATATTAACATTTGGT+TGG | - | Chr7:24007288-24007307 | None:intergenic | 25.0% |
| AAAGAGGAAACTATACTACT+TGG | + | Chr7:24007112-24007131 | MsG0780037345.01.T01:CDS | 30.0% | |
| ATTTCATGAAGAAGTCGTTT+AGG | + | Chr7:24007374-24007393 | MsG0780037345.01.T01:CDS | 30.0% | |
| TTCAGAGTGTGATTATGAAA+TGG | + | Chr7:24007315-24007334 | MsG0780037345.01.T01:CDS | 30.0% | |
| AGAGTTCAGTTAAGAACCAT+TGG | + | Chr7:24007200-24007219 | MsG0780037345.01.T01:CDS | 35.0% | |
| CAAGAACAACTCAATGAACA+CGG | + | Chr7:24007249-24007268 | MsG0780037345.01.T01:CDS | 35.0% | |
| GAAATTGCTAAGAGATTTCC+AGG | + | Chr7:24007169-24007188 | MsG0780037345.01.T01:CDS | 35.0% | |
| TCTTAACTGAACTCTCAGTA+CGG | - | Chr7:24007195-24007214 | None:intergenic | 35.0% | |
| !! | ACATTGTTTTGTATGCTTGC+AGG | + | Chr7:24007072-24007091 | MsG0780037345.01.T01:CDS | 35.0% |
| !!! | GTTTCCTCTTTTTTACTCCA+TGG | - | Chr7:24007103-24007122 | None:intergenic | 35.0% |
| !!! | TGAAATGGAGTTTTTTCCTG+AGG | + | Chr7:24007330-24007349 | MsG0780037345.01.T01:CDS | 35.0% |
| CGAACCATGGAGTAAAAAAG+AGG | + | Chr7:24007096-24007115 | MsG0780037345.01.T01:CDS | 40.0% | |
| ! | CGTGTTCATTGAGTTGTTCT+TGG | - | Chr7:24007251-24007270 | None:intergenic | 40.0% |
| TCCTGCTGCATAAAAACCTC+AGG | - | Chr7:24007349-24007368 | None:intergenic | 45.0% | |
| ! | TCCTGAGGTTTTTATGCAGC+AGG | + | Chr7:24007345-24007364 | MsG0780037345.01.T01:CDS | 45.0% |
| CACGCTCAATGGCATTCCAA+TGG | - | Chr7:24007219-24007238 | None:intergenic | 50.0% | |
| GGAATGCCATTGAGCGTGTT+CGG | + | Chr7:24007221-24007240 | MsG0780037345.01.T01:CDS | 50.0% | |
| TATGCTTGCAGGACGAACCA+TGG | + | Chr7:24007083-24007102 | MsG0780037345.01.T01:CDS | 50.0% | |
| ACTGAACTCTCAGTACGGCC+TGG | - | Chr7:24007190-24007209 | None:intergenic | 55.0% | |
| GGCATTCCGAACACGCTCAA+TGG | - | Chr7:24007230-24007249 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 24007025 | 24007405 | 24007025 | ID=MsG0780037345.01;Name=MsG0780037345.01 |
| Chr7 | mRNA | 24007025 | 24007405 | 24007025 | ID=MsG0780037345.01.T01;Parent=MsG0780037345.01;Name=MsG0780037345.01.T01;_AED=0.09;_eAED=0.10;_QI=0|-1|0|1|-1|1|1|0|126 |
| Chr7 | exon | 24007025 | 24007405 | 24007025 | ID=MsG0780037345.01.T01:exon:6452;Parent=MsG0780037345.01.T01 |
| Chr7 | CDS | 24007025 | 24007405 | 24007025 | ID=MsG0780037345.01.T01:cds;Parent=MsG0780037345.01.T01 |
| Gene Sequence |
| Protein sequence |
