AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780037788.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780037788.01.T01 MTR_1g021440 63.393 224 73 3 9 229 37 254 2.18e-92 273
MsG0780037788.01.T01 MTR_1g021320 59.551 267 61 4 9 229 197 462 7.02e-92 287
MsG0780037788.01.T01 MTR_1g021320 75.000 124 31 0 9 132 40 163 2.06e-58 197
MsG0780037788.01.T01 MTR_1g021440 63.393 224 73 3 9 229 37 254 7.34e-91 273
MsG0780037788.01.T01 MTR_1g021440 65.686 204 62 2 9 209 37 235 1.30e-89 266
MsG0780037788.01.T01 MTR_1g021440 65.686 204 62 2 9 209 37 235 4.02e-88 265
MsG0780037788.01.T01 MTR_1g021350 60.793 227 61 6 9 229 36 240 9.84e-82 248
MsG0780037788.01.T01 MTR_1g021330 58.333 240 60 6 2 229 23 234 6.23e-80 244
MsG0780037788.01.T01 MTR_1g021435 58.667 225 50 5 9 229 37 222 6.90e-74 229
MsG0780037788.01.T01 MTR_1g021400 56.757 222 58 3 9 229 45 229 5.34e-70 219
MsG0780037788.01.T01 MTR_1g021380 54.911 224 54 5 9 229 36 215 1.50e-68 214
MsG0780037788.01.T01 MTR_1g021410 56.306 222 60 5 9 229 37 222 1.45e-64 204
MsG0780037788.01.T01 MTR_1g021500 50.661 227 80 6 9 222 41 248 2.13e-58 191
MsG0780037788.01.T01 MTR_1g021500 51.982 227 77 8 9 222 41 248 3.51e-58 191
MsG0780037788.01.T01 MTR_1g021360 72.000 125 35 0 9 133 40 164 4.06e-57 188
MsG0780037788.01.T01 MTR_1g021360 52.941 204 44 4 9 211 197 349 5.07e-57 188
MsG0780037788.01.T01 MTR_1g021290 45.946 222 76 4 9 230 170 347 1.54e-50 168
MsG0780037788.01.T01 MTR_1g021290 74.257 101 26 0 9 109 42 142 1.80e-47 160
MsG0780037788.01.T01 MTR_1g021270 37.209 86 53 1 10 95 31 115 9.69e-14 70.1
MsG0780037788.01.T01 MTR_3g098790 36.709 79 50 0 10 88 29 107 7.13e-11 61.2
MsG0780037788.01.T01 MTR_7g050710 36.486 74 47 0 10 83 29 102 7.29e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780037788.01.T01 AT4G33280 55.208 96 42 1 9 103 38 133 4.67e-29 110
MsG0780037788.01.T01 AT4G33280 55.208 96 42 1 9 103 38 133 1.52e-28 110
MsG0780037788.01.T01 AT4G33280 55.208 96 42 1 9 103 38 133 2.33e-28 110
MsG0780037788.01.T01 AT4G34400 36.471 85 54 0 7 91 28 112 6.73e-16 76.3
MsG0780037788.01.T01 AT4G34400 36.471 85 54 0 7 91 28 112 6.73e-16 76.3
MsG0780037788.01.T01 AT3G06220 28.182 110 71 2 10 119 25 126 7.56e-13 65.1
MsG0780037788.01.T01 AT5G60130 36.275 102 53 3 2 91 14 115 2.08e-12 65.1
MsG0780037788.01.T01 AT5G60130 36.275 102 53 3 2 91 11 112 6.05e-12 64.3
MsG0780037788.01.T01 AT5G60130 36.275 102 53 3 2 91 14 115 6.20e-12 64.3
MsG0780037788.01.T01 AT3G18990 33.673 98 56 1 10 98 22 119 6.59e-12 64.7
MsG0780037788.01.T01 AT5G60130 36.275 102 53 3 2 91 14 115 7.24e-12 63.9
MsG0780037788.01.T01 AT5G60130 38.636 88 49 2 9 91 2 89 7.75e-12 63.9
MsG0780037788.01.T01 AT3G18990 33.673 98 56 1 10 98 22 119 7.93e-12 64.3
MsG0780037788.01.T01 AT3G06160 35.526 76 49 0 8 83 38 113 1.84e-11 63.2
MsG0780037788.01.T01 AT3G06160 35.526 76 49 0 8 83 23 98 2.04e-11 62.8
MsG0780037788.01.T01 AT3G06160 35.526 76 49 0 8 83 23 98 2.04e-11 62.8
MsG0780037788.01.T01 AT3G06160 35.526 76 49 0 8 83 23 98 2.04e-11 62.8
MsG0780037788.01.T01 AT3G06160 36.000 75 48 0 9 83 83 157 2.66e-11 62.8

Find 47 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACTCCACTAGGACCTTTA+AGG 0.178570 7:-33214291 None:intergenic
CCTGAAAATGTGACCCTTAA+AGG 0.191053 7:+33214277 MsG0780037788.01.T01:CDS
GCTGGTCTTCCCCGCTTCTT+AGG 0.270434 7:-33214811 None:intergenic
AACTTCGTTAACAGGCTTCT+TGG 0.341598 7:-33214776 None:intergenic
GTTTATATACATTTATTTGC+AGG 0.351558 7:+33214213 MsG0780037788.01.T01:CDS
CCAGGCGTAACTTCGTTAAC+AGG 0.352395 7:-33214784 None:intergenic
GTCTTCCCCGCTTCTTAGGC+TGG 0.353235 7:-33214807 None:intergenic
AATGCGGTTCTGAGAAATTT+CGG 0.363117 7:+33214602 MsG0780037788.01.T01:CDS
CCCCGCTTCTTAGGCTGGCC+AGG 0.381562 7:-33214802 None:intergenic
CCAGCAAAATCAGCTACTTC+TGG 0.385623 7:+33214829 MsG0780037788.01.T01:CDS
GTGGAGTTGTGTGGAATATA+TGG 0.388254 7:+33214305 MsG0780037788.01.T01:CDS
TTTGTTAGAAAATGTGGAAA+AGG 0.421612 7:+33214496 MsG0780037788.01.T01:CDS
GCGAGAAAGCAATTGTTGAT+TGG 0.450466 7:+33214851 MsG0780037788.01.T01:CDS
GAAGTCAACACTGCTTCTAA+TGG 0.464985 7:+33214571 MsG0780037788.01.T01:CDS
GCCTGGCCAGCCTAAGAAGC+GGG 0.466869 7:+33214801 MsG0780037788.01.T01:CDS
AGAGCCCGCTTTCTCGCATA+AGG 0.473371 7:-33214470 None:intergenic
TCTTACTTTGTTAGAAAATG+TGG 0.473474 7:+33214490 MsG0780037788.01.T01:CDS
AACTCCACTAGGACCTTTAA+GGG 0.479312 7:-33214290 None:intergenic
CGAGAAAGCAATTGTTGATT+GGG 0.485091 7:+33214852 MsG0780037788.01.T01:CDS
GCGGTTCCCTCCCAAACAAC+CGG 0.485847 7:+33214745 MsG0780037788.01.T01:CDS
GCATCAGCCATGACCTGCTC+AGG 0.490883 7:-33214712 None:intergenic
TACTTCTCCTGAGCAGGTCA+TGG 0.497650 7:+33214705 MsG0780037788.01.T01:CDS
TGAGTCTACTTCTCCTGAGC+AGG 0.520458 7:+33214699 MsG0780037788.01.T01:CDS
TACTTCACAAACAGTTGGCA+AGG 0.525029 7:+33214352 MsG0780037788.01.T01:CDS
AATGAAAAGACTTCTAATGC+TGG 0.525954 7:+33214673 MsG0780037788.01.T01:CDS
CCTGTTAACGAAGTTACGCC+TGG 0.535294 7:+33214784 MsG0780037788.01.T01:CDS
CTGTTTACTTCACAAACAGT+TGG 0.562500 7:+33214347 MsG0780037788.01.T01:CDS
CGCCTGGCCAGCCTAAGAAG+CGG 0.563693 7:+33214800 MsG0780037788.01.T01:CDS
GAAAGCAATTGTTGATTGGG+TGG 0.568458 7:+33214855 MsG0780037788.01.T01:CDS
TATATGGCTGACAACTAAAG+AGG 0.582468 7:+33214321 MsG0780037788.01.T01:CDS
GAATGATCACTCTTTGAAAG+AGG 0.588036 7:+33214381 MsG0780037788.01.T01:CDS
GAGACCTTATGCGAGAAAGC+GGG 0.590946 7:+33214466 MsG0780037788.01.T01:CDS
ATATTCCACACAACTCCACT+AGG 0.593357 7:-33214301 None:intergenic
GGCTGATGCAGTCACTATTG+CGG 0.596142 7:+33214726 MsG0780037788.01.T01:CDS
TACTGAGCAAGAGGGAGACA+AGG 0.597506 7:+33214522 MsG0780037788.01.T01:CDS
AAAAGGTCATACTGAGCAAG+AGG 0.610370 7:+33214513 MsG0780037788.01.T01:CDS
AAGGTCCTAGTGGAGTTGTG+TGG 0.624686 7:+33214296 MsG0780037788.01.T01:CDS
TGAGACCTTATGCGAGAAAG+CGG 0.630542 7:+33214465 MsG0780037788.01.T01:CDS
GTGACCCTTAAAGGTCCTAG+TGG 0.630968 7:+33214286 MsG0780037788.01.T01:CDS
GCTTGTAGCAAGGAAAACTC+AGG 0.631925 7:+33214877 MsG0780037788.01.T01:CDS
CCTGGCCAGCCTAAGAAGCG+GGG 0.640983 7:+33214802 MsG0780037788.01.T01:CDS
GCAAGAGGGAGACAAGGCAA+AGG 0.642426 7:+33214528 MsG0780037788.01.T01:CDS
GTCAACACTGCTTCTAATGG+CGG 0.645750 7:+33214574 MsG0780037788.01.T01:CDS
AATGATCACTCTTTGAAAGA+GGG 0.654505 7:+33214382 MsG0780037788.01.T01:CDS
TGATTGGGTGGCTTGTAGCA+AGG 0.654638 7:+33214867 MsG0780037788.01.T01:CDS
TCTAATGGCGGTGTTGAATG+CGG 0.661026 7:+33214586 MsG0780037788.01.T01:CDS
AAAGGTCATACTGAGCAAGA+GGG 0.680883 7:+33214514 MsG0780037788.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGAAGTTTTAATATTTCA+TGG + Chr7:33214442-33214461 MsG0780037788.01.T01:CDS 15.0%
!! GTTTATATACATTTATTTGC+AGG + Chr7:33214213-33214232 MsG0780037788.01.T01:CDS 20.0%
! TCTTACTTTGTTAGAAAATG+TGG + Chr7:33214490-33214509 MsG0780037788.01.T01:CDS 25.0%
! TTTGTTAGAAAATGTGGAAA+AGG + Chr7:33214496-33214515 MsG0780037788.01.T01:CDS 25.0%
!! AAATTGGCTGAAAATGTTTT+AGG - Chr7:33214244-33214263 None:intergenic 25.0%
!! TTTTCATTGGTATTTTCGAA+AGG - Chr7:33214661-33214680 None:intergenic 25.0%
!!! AGGCAACTTTTTCTTTAAAT+TGG - Chr7:33214260-33214279 None:intergenic 25.0%
AATGAAAAGACTTCTAATGC+TGG + Chr7:33214673-33214692 MsG0780037788.01.T01:CDS 30.0%
! AATGATCACTCTTTGAAAGA+GGG + Chr7:33214382-33214401 MsG0780037788.01.T01:CDS 30.0%
! AGCATTAGAAGTCTTTTCAT+TGG - Chr7:33214674-33214693 None:intergenic 30.0%
!!! CTCCTTTTTTTCAAGTACAA+TGG + Chr7:33214409-33214428 MsG0780037788.01.T01:CDS 30.0%
CGCCATTGTACTTGAAAAAA+AGG - Chr7:33214414-33214433 None:intergenic 35.0%
CTGTTTACTTCACAAACAGT+TGG + Chr7:33214347-33214366 MsG0780037788.01.T01:CDS 35.0%
TATATGGCTGACAACTAAAG+AGG + Chr7:33214321-33214340 MsG0780037788.01.T01:CDS 35.0%
! CGAGAAAGCAATTGTTGATT+GGG + Chr7:33214852-33214871 MsG0780037788.01.T01:CDS 35.0%
! GAATGATCACTCTTTGAAAG+AGG + Chr7:33214381-33214400 MsG0780037788.01.T01:CDS 35.0%
!! AATGCGGTTCTGAGAAATTT+CGG + Chr7:33214602-33214621 MsG0780037788.01.T01:CDS 35.0%
!! TTTTCGAAAGGTACAGCTAA+TGG - Chr7:33214649-33214668 None:intergenic 35.0%
AAAAGGTCATACTGAGCAAG+AGG + Chr7:33214513-33214532 MsG0780037788.01.T01:CDS 40.0%
AAAGGTCATACTGAGCAAGA+GGG + Chr7:33214514-33214533 MsG0780037788.01.T01:CDS 40.0%
AACTCCACTAGGACCTTTAA+GGG - Chr7:33214293-33214312 None:intergenic 40.0%
AACTTCGTTAACAGGCTTCT+TGG - Chr7:33214779-33214798 None:intergenic 40.0%
ATATTCCACACAACTCCACT+AGG - Chr7:33214304-33214323 None:intergenic 40.0%
CCTGAAAATGTGACCCTTAA+AGG + Chr7:33214277-33214296 MsG0780037788.01.T01:CDS 40.0%
GAAGTCAACACTGCTTCTAA+TGG + Chr7:33214571-33214590 MsG0780037788.01.T01:CDS 40.0%
GCGAGAAAGCAATTGTTGAT+TGG + Chr7:33214851-33214870 MsG0780037788.01.T01:CDS 40.0%
GTGGAGTTGTGTGGAATATA+TGG + Chr7:33214305-33214324 MsG0780037788.01.T01:CDS 40.0%
TACTTCACAAACAGTTGGCA+AGG + Chr7:33214352-33214371 MsG0780037788.01.T01:CDS 40.0%
! GAAAGCAATTGTTGATTGGG+TGG + Chr7:33214855-33214874 MsG0780037788.01.T01:CDS 40.0%
!! CCTTTAAGGGTCACATTTTC+AGG - Chr7:33214280-33214299 None:intergenic 40.0%
CAACTCCACTAGGACCTTTA+AGG - Chr7:33214294-33214313 None:intergenic 45.0%
CCAGCAAAATCAGCTACTTC+TGG + Chr7:33214829-33214848 MsG0780037788.01.T01:CDS 45.0%
GCTTGTAGCAAGGAAAACTC+AGG + Chr7:33214877-33214896 MsG0780037788.01.T01:CDS 45.0%
GTCAACACTGCTTCTAATGG+CGG + Chr7:33214574-33214593 MsG0780037788.01.T01:CDS 45.0%
TGAGACCTTATGCGAGAAAG+CGG + Chr7:33214465-33214484 MsG0780037788.01.T01:CDS 45.0%
! TCTAATGGCGGTGTTGAATG+CGG + Chr7:33214586-33214605 MsG0780037788.01.T01:CDS 45.0%
!! AGGCTTCTTGGTCTTTTTGC+CGG - Chr7:33214767-33214786 None:intergenic 45.0%
!! CCAGAAGTAGCTGATTTTGC+TGG - Chr7:33214832-33214851 None:intergenic 45.0%
!! GGTCTTTTTGCCGGTTGTTT+GGG - Chr7:33214758-33214777 None:intergenic 45.0%
!! TGGTCTTTTTGCCGGTTGTT+TGG - Chr7:33214759-33214778 None:intergenic 45.0%
!! TTTTTGCCGGTTGTTTGGGA+GGG - Chr7:33214754-33214773 None:intergenic 45.0%
AAGGTCCTAGTGGAGTTGTG+TGG + Chr7:33214296-33214315 MsG0780037788.01.T01:CDS 50.0%
CCAGGCGTAACTTCGTTAAC+AGG - Chr7:33214787-33214806 None:intergenic 50.0%
CCTGTTAACGAAGTTACGCC+TGG + Chr7:33214784-33214803 MsG0780037788.01.T01:CDS 50.0%
GAGACCTTATGCGAGAAAGC+GGG + Chr7:33214466-33214485 MsG0780037788.01.T01:CDS 50.0%
GGCTGATGCAGTCACTATTG+CGG + Chr7:33214726-33214745 MsG0780037788.01.T01:CDS 50.0%
GTGACCCTTAAAGGTCCTAG+TGG + Chr7:33214286-33214305 MsG0780037788.01.T01:CDS 50.0%
TACTGAGCAAGAGGGAGACA+AGG + Chr7:33214522-33214541 MsG0780037788.01.T01:CDS 50.0%
TACTTCTCCTGAGCAGGTCA+TGG + Chr7:33214705-33214724 MsG0780037788.01.T01:CDS 50.0%
TGAGTCTACTTCTCCTGAGC+AGG + Chr7:33214699-33214718 MsG0780037788.01.T01:CDS 50.0%
! TGATTGGGTGGCTTGTAGCA+AGG + Chr7:33214867-33214886 MsG0780037788.01.T01:CDS 50.0%
!! CTTTTTGCCGGTTGTTTGGG+AGG - Chr7:33214755-33214774 None:intergenic 50.0%
AGAGCCCGCTTTCTCGCATA+AGG - Chr7:33214473-33214492 None:intergenic 55.0%
GCAAGAGGGAGACAAGGCAA+AGG + Chr7:33214528-33214547 MsG0780037788.01.T01:CDS 55.0%
GCATCAGCCATGACCTGCTC+AGG - Chr7:33214715-33214734 None:intergenic 60.0%
GCGGTTCCCTCCCAAACAAC+CGG + Chr7:33214745-33214764 MsG0780037788.01.T01:CDS 60.0%
GTCTTCCCCGCTTCTTAGGC+TGG - Chr7:33214810-33214829 None:intergenic 60.0%
!! GCTGGTCTTCCCCGCTTCTT+AGG - Chr7:33214814-33214833 None:intergenic 60.0%
CCTGGCCAGCCTAAGAAGCG+GGG + Chr7:33214802-33214821 MsG0780037788.01.T01:CDS 65.0%
CGCCTGGCCAGCCTAAGAAG+CGG + Chr7:33214800-33214819 MsG0780037788.01.T01:CDS 65.0%
GCCTGGCCAGCCTAAGAAGC+GGG + Chr7:33214801-33214820 MsG0780037788.01.T01:CDS 65.0%
! CCCCGCTTCTTAGGCTGGCC+AGG - Chr7:33214805-33214824 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr7 gene 33214211 33214903 33214211 ID=MsG0780037788.01;Name=MsG0780037788.01
Chr7 mRNA 33214211 33214903 33214211 ID=MsG0780037788.01.T01;Parent=MsG0780037788.01;Name=MsG0780037788.01.T01;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|230
Chr7 exon 33214211 33214903 33214211 ID=MsG0780037788.01.T01:exon:912;Parent=MsG0780037788.01.T01
Chr7 CDS 33214211 33214903 33214211 ID=MsG0780037788.01.T01:cds;Parent=MsG0780037788.01.T01
Gene Sequence

>MsG0780037788.01.T01

ATGTTTATATACATTTATTTGCAGGCACTTCCTAAAACATTTTCAGCCAATTTAAAGAAAAAGTTGCCTGAAAATGTGACCCTTAAAGGTCCTAGTGGAGTTGTGTGGAATATATGGCTGACAACTAAAGAGGACACTGTTTACTTCACAAACAGTTGGCAAGGATTTGTGAATGATCACTCTTTGAAAGAGGGTGATCTCCTTTTTTTCAAGTACAATGGCGAATCGTTGTTTGAAGTTTTAATATTTCATGGTGAGACCTTATGCGAGAAAGCGGGCTCTTACTTTGTTAGAAAATGTGGAAAAGGTCATACTGAGCAAGAGGGAGACAAGGCAAAGGTTGCAAAGAATTCTGTTGAAGAAGTCAACACTGCTTCTAATGGCGGTGTTGAATGCGGTTCTGAGAAATTTCGGAAGCTTGACACTATAAGAACACCATTAGCTGTACCTTTCGAAAATACCAATGAAAAGACTTCTAATGCTGGAGTTGAGTCTACTTCTCCTGAGCAGGTCATGGCTGATGCAGTCACTATTGCGGTTCCCTCCCAAACAACCGGCAAAAAGACCAAGAAGCCTGTTAACGAAGTTACGCCTGGCCAGCCTAAGAAGCGGGGAAGACCAGCAAAATCAGCTACTTCTGGCGAGAAAGCAATTGTTGATTGGGTGGCTTGTAGCAAGGAAAACTCAGGTTAA

Protein sequence

>MsG0780037788.01.T01

MFIYIYLQALPKTFSANLKKKLPENVTLKGPSGVVWNIWLTTKEDTVYFTNSWQGFVNDHSLKEGDLLFFKYNGESLFEVLIFHGETLCEKAGSYFVRKCGKGHTEQEGDKAKVAKNSVEEVNTASNGGVECGSEKFRKLDTIRTPLAVPFENTNEKTSNAGVESTSPEQVMADAVTIAVPSQTTGKKTKKPVNEVTPGQPKKRGRPAKSATSGEKAIVDWVACSKENSG*