Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037883.01.T01 | XP_039688775.1 | 95.971 | 273 | 10 | 1 | 1 | 272 | 1 | 273 | 0 | 552 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037883.01.T01 | Q9SWG3 | 49.573 | 234 | 113 | 2 | 39 | 267 | 50 | 283 | 6.60E-73 | 243 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037883.01.T01 | A0A2Z6NS90 | 75.636 | 275 | 66 | 1 | 1 | 274 | 1 | 275 | 3.50e-147 | 435 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0780037883.01.T01 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037883.01.T01 | MTR_1g008290 | 68.571 | 140 | 41 | 1 | 93 | 232 | 8 | 144 | 1.79e-68 | 211 |
| MsG0780037883.01.T01 | MTR_5g032260 | 62.037 | 108 | 41 | 0 | 67 | 174 | 299 | 406 | 1.46e-39 | 144 |
| MsG0780037883.01.T01 | MTR_1g070355 | 30.000 | 270 | 169 | 7 | 13 | 270 | 48 | 309 | 2.10e-32 | 127 |
| MsG0780037883.01.T01 | MTR_6g051425 | 27.143 | 280 | 177 | 5 | 29 | 291 | 6 | 275 | 2.58e-31 | 120 |
| MsG0780037883.01.T01 | MTR_7g446520 | 29.520 | 271 | 172 | 6 | 8 | 277 | 62 | 314 | 5.45e-28 | 111 |
| MsG0780037883.01.T01 | MTR_5g076070 | 27.953 | 254 | 159 | 8 | 24 | 264 | 40 | 282 | 1.73e-27 | 108 |
| MsG0780037883.01.T01 | MTR_7g446480 | 36.842 | 152 | 95 | 1 | 122 | 272 | 151 | 302 | 7.97e-26 | 105 |
| MsG0780037883.01.T01 | MTR_7g446510 | 34.969 | 163 | 105 | 1 | 122 | 283 | 166 | 328 | 9.99e-25 | 102 |
| MsG0780037883.01.T01 | MTR_4g035820 | 29.861 | 288 | 179 | 8 | 2 | 271 | 33 | 315 | 6.71e-23 | 99.8 |
| MsG0780037883.01.T01 | MTR_0402s0040 | 30.387 | 181 | 117 | 3 | 40 | 213 | 26 | 204 | 2.22e-21 | 94.7 |
| MsG0780037883.01.T01 | MTR_0558s0020 | 27.626 | 257 | 157 | 7 | 43 | 272 | 39 | 293 | 4.73e-19 | 86.3 |
| MsG0780037883.01.T01 | MTR_2g098880 | 35.849 | 106 | 62 | 1 | 39 | 138 | 60 | 165 | 1.26e-18 | 84.3 |
| MsG0780037883.01.T01 | MTR_2g098880 | 35.849 | 106 | 62 | 1 | 39 | 138 | 72 | 177 | 1.65e-18 | 84.0 |
| MsG0780037883.01.T01 | MTR_1g013060 | 29.808 | 208 | 135 | 6 | 39 | 236 | 23 | 229 | 2.53e-17 | 80.1 |
| MsG0780037883.01.T01 | MTR_8g446020 | 30.409 | 171 | 113 | 3 | 103 | 269 | 81 | 249 | 4.52e-16 | 77.0 |
| MsG0780037883.01.T01 | MTR_4g067290 | 33.621 | 116 | 72 | 2 | 28 | 139 | 44 | 158 | 2.27e-15 | 74.7 |
| MsG0780037883.01.T01 | MTR_4g067290 | 33.621 | 116 | 72 | 2 | 28 | 139 | 64 | 178 | 2.51e-15 | 75.1 |
| MsG0780037883.01.T01 | MTR_4g067290 | 34.821 | 112 | 68 | 2 | 32 | 139 | 66 | 176 | 2.69e-15 | 74.7 |
| MsG0780037883.01.T01 | MTR_4g067290 | 33.621 | 116 | 72 | 2 | 28 | 139 | 44 | 158 | 2.74e-15 | 74.7 |
| MsG0780037883.01.T01 | MTR_4g067290 | 34.821 | 112 | 68 | 2 | 32 | 139 | 68 | 178 | 3.10e-15 | 74.7 |
| MsG0780037883.01.T01 | MTR_4g067290 | 34.821 | 112 | 68 | 2 | 32 | 139 | 108 | 218 | 5.07e-15 | 74.7 |
| MsG0780037883.01.T01 | MTR_3g006370 | 34.862 | 109 | 66 | 1 | 35 | 138 | 45 | 153 | 5.39e-15 | 73.6 |
| MsG0780037883.01.T01 | MTR_3g450850 | 27.572 | 243 | 149 | 8 | 43 | 264 | 50 | 286 | 1.79e-14 | 73.6 |
| MsG0780037883.01.T01 | MTR_7g446060 | 37.647 | 85 | 53 | 0 | 121 | 205 | 6 | 90 | 9.82e-14 | 67.8 |
| MsG0780037883.01.T01 | MTR_6g090505 | 26.522 | 230 | 142 | 6 | 84 | 289 | 19 | 245 | 1.90e-13 | 70.9 |
| MsG0780037883.01.T01 | MTR_2g098920 | 36.190 | 105 | 62 | 2 | 39 | 139 | 79 | 182 | 1.96e-13 | 69.7 |
| MsG0780037883.01.T01 | MTR_5g015480 | 32.000 | 125 | 79 | 3 | 40 | 158 | 56 | 180 | 2.26e-12 | 66.6 |
| MsG0780037883.01.T01 | MTR_0856s0010 | 30.645 | 124 | 83 | 2 | 39 | 160 | 33 | 155 | 6.68e-11 | 60.5 |
| MsG0780037883.01.T01 | MTR_1g019850 | 28.481 | 158 | 92 | 5 | 26 | 169 | 48 | 198 | 7.63e-11 | 61.6 |
| MsG0780037883.01.T01 | MTR_1g083640 | 24.783 | 230 | 145 | 6 | 44 | 247 | 55 | 282 | 8.24e-11 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037883.01.T01 | AT4G15090 | 49.573 | 234 | 113 | 2 | 39 | 267 | 50 | 283 | 6.72e-74 | 243 |
| MsG0780037883.01.T01 | AT4G15090 | 49.573 | 234 | 113 | 2 | 39 | 267 | 50 | 283 | 6.72e-74 | 243 |
| MsG0780037883.01.T01 | AT4G15090 | 49.573 | 234 | 113 | 2 | 39 | 267 | 50 | 283 | 6.72e-74 | 243 |
| MsG0780037883.01.T01 | AT4G15090 | 49.573 | 234 | 113 | 2 | 39 | 267 | 50 | 283 | 6.72e-74 | 243 |
| MsG0780037883.01.T01 | AT4G15090 | 49.573 | 234 | 113 | 2 | 39 | 267 | 50 | 283 | 6.72e-74 | 243 |
| MsG0780037883.01.T01 | AT1G76320 | 47.414 | 232 | 114 | 3 | 44 | 273 | 1 | 226 | 7.13e-68 | 224 |
| MsG0780037883.01.T01 | AT1G76320 | 47.414 | 232 | 114 | 3 | 44 | 273 | 1 | 226 | 9.63e-68 | 225 |
| MsG0780037883.01.T01 | AT1G76320 | 47.414 | 232 | 114 | 3 | 44 | 273 | 1 | 226 | 9.73e-68 | 225 |
| MsG0780037883.01.T01 | AT1G76320 | 47.414 | 232 | 114 | 3 | 44 | 273 | 1 | 226 | 9.73e-68 | 225 |
| MsG0780037883.01.T01 | AT3G22170 | 43.825 | 251 | 102 | 3 | 39 | 272 | 69 | 297 | 2.08e-64 | 218 |
| MsG0780037883.01.T01 | AT3G22170 | 43.825 | 251 | 102 | 3 | 39 | 272 | 69 | 297 | 2.08e-64 | 218 |
| MsG0780037883.01.T01 | AT2G32250 | 43.388 | 242 | 97 | 4 | 43 | 283 | 41 | 243 | 1.79e-49 | 176 |
| MsG0780037883.01.T01 | AT2G32250 | 44.589 | 231 | 88 | 4 | 43 | 272 | 41 | 232 | 2.72e-49 | 176 |
| MsG0780037883.01.T01 | AT2G32250 | 44.589 | 231 | 88 | 4 | 43 | 272 | 41 | 232 | 2.72e-49 | 176 |
| MsG0780037883.01.T01 | AT2G32250 | 44.589 | 231 | 88 | 4 | 43 | 272 | 41 | 232 | 2.72e-49 | 176 |
| MsG0780037883.01.T01 | AT2G32250 | 44.589 | 231 | 88 | 4 | 43 | 272 | 41 | 232 | 2.77e-49 | 176 |
| MsG0780037883.01.T01 | AT2G32250 | 44.589 | 231 | 88 | 4 | 43 | 272 | 41 | 232 | 2.77e-49 | 176 |
| MsG0780037883.01.T01 | AT2G32250 | 43.154 | 241 | 97 | 4 | 44 | 283 | 1 | 202 | 1.44e-48 | 174 |
| MsG0780037883.01.T01 | AT2G32250 | 43.154 | 241 | 97 | 4 | 44 | 283 | 1 | 202 | 1.52e-48 | 174 |
| MsG0780037883.01.T01 | AT4G38180 | 37.945 | 253 | 148 | 3 | 39 | 283 | 72 | 323 | 1.05e-47 | 171 |
| MsG0780037883.01.T01 | AT4G19990 | 35.145 | 276 | 123 | 4 | 29 | 283 | 6 | 246 | 3.34e-46 | 167 |
| MsG0780037883.01.T01 | AT4G19990 | 35.145 | 276 | 123 | 4 | 29 | 283 | 6 | 246 | 3.34e-46 | 167 |
| MsG0780037883.01.T01 | AT4G19990 | 35.145 | 276 | 123 | 4 | 29 | 283 | 6 | 246 | 4.00e-46 | 167 |
| MsG0780037883.01.T01 | AT4G19990 | 35.145 | 276 | 123 | 4 | 29 | 283 | 20 | 260 | 4.20e-46 | 167 |
| MsG0780037883.01.T01 | AT4G19990 | 35.145 | 276 | 123 | 4 | 29 | 283 | 6 | 246 | 5.20e-46 | 166 |
| MsG0780037883.01.T01 | AT4G19990 | 35.145 | 276 | 123 | 4 | 29 | 283 | 6 | 246 | 5.20e-46 | 166 |
| MsG0780037883.01.T01 | AT1G80010 | 35.417 | 240 | 146 | 3 | 40 | 271 | 95 | 333 | 1.52e-36 | 139 |
| MsG0780037883.01.T01 | AT1G52520 | 29.452 | 292 | 188 | 3 | 2 | 283 | 38 | 321 | 2.90e-32 | 127 |
| MsG0780037883.01.T01 | AT4G19990 | 33.937 | 221 | 94 | 3 | 80 | 283 | 4 | 189 | 1.38e-31 | 125 |
| MsG0780037883.01.T01 | AT3G06250 | 30.556 | 252 | 136 | 5 | 21 | 271 | 174 | 387 | 6.81e-28 | 114 |
| MsG0780037883.01.T01 | AT3G06250 | 30.435 | 115 | 71 | 1 | 23 | 137 | 11 | 116 | 2.64e-12 | 68.2 |
| MsG0780037883.01.T01 | AT3G06250 | 30.556 | 252 | 136 | 5 | 21 | 271 | 174 | 387 | 6.81e-28 | 114 |
| MsG0780037883.01.T01 | AT3G06250 | 30.435 | 115 | 71 | 1 | 23 | 137 | 11 | 116 | 2.64e-12 | 68.2 |
| MsG0780037883.01.T01 | AT3G06250 | 30.556 | 252 | 136 | 5 | 21 | 271 | 174 | 387 | 6.81e-28 | 114 |
| MsG0780037883.01.T01 | AT3G06250 | 30.435 | 115 | 71 | 1 | 23 | 137 | 11 | 116 | 2.64e-12 | 68.2 |
| MsG0780037883.01.T01 | AT2G27110 | 29.200 | 250 | 151 | 6 | 36 | 283 | 45 | 270 | 4.25e-27 | 112 |
| MsG0780037883.01.T01 | AT2G27110 | 29.200 | 250 | 151 | 6 | 36 | 283 | 45 | 270 | 4.25e-27 | 112 |
| MsG0780037883.01.T01 | AT5G18960 | 30.279 | 251 | 138 | 5 | 24 | 271 | 195 | 411 | 2.88e-26 | 109 |
| MsG0780037883.01.T01 | AT5G18960 | 33.628 | 113 | 66 | 1 | 25 | 137 | 28 | 131 | 6.30e-13 | 70.1 |
| MsG0780037883.01.T01 | AT5G18960 | 30.279 | 251 | 138 | 5 | 24 | 271 | 195 | 411 | 2.88e-26 | 109 |
| MsG0780037883.01.T01 | AT5G18960 | 33.628 | 113 | 66 | 1 | 25 | 137 | 28 | 131 | 6.30e-13 | 70.1 |
| MsG0780037883.01.T01 | AT1G10240 | 26.909 | 275 | 184 | 8 | 5 | 266 | 14 | 284 | 1.33e-24 | 104 |
| MsG0780037883.01.T01 | AT1G10240 | 26.909 | 275 | 184 | 8 | 5 | 266 | 14 | 284 | 1.33e-24 | 104 |
| MsG0780037883.01.T01 | AT5G28530 | 26.275 | 255 | 160 | 9 | 40 | 271 | 55 | 304 | 3.91e-19 | 88.6 |
| MsG0780037883.01.T01 | AT5G28530 | 26.275 | 255 | 160 | 9 | 40 | 271 | 55 | 304 | 4.09e-19 | 88.6 |
| MsG0780037883.01.T01 | AT5G28530 | 26.275 | 255 | 160 | 9 | 40 | 271 | 55 | 304 | 4.09e-19 | 88.6 |
| MsG0780037883.01.T01 | AT3G07500 | 37.956 | 137 | 75 | 4 | 12 | 139 | 1 | 136 | 1.66e-16 | 77.8 |
| MsG0780037883.01.T01 | AT3G59470 | 32.759 | 116 | 75 | 1 | 28 | 140 | 70 | 185 | 3.86e-16 | 77.4 |
| MsG0780037883.01.T01 | AT3G59470 | 32.759 | 116 | 75 | 1 | 28 | 140 | 58 | 173 | 3.91e-16 | 77.4 |
| MsG0780037883.01.T01 | AT3G59470 | 32.759 | 116 | 75 | 1 | 28 | 140 | 58 | 173 | 3.91e-16 | 77.4 |
| MsG0780037883.01.T01 | AT2G27110 | 42.667 | 75 | 43 | 0 | 209 | 283 | 51 | 125 | 5.63e-15 | 76.3 |
| MsG0780037883.01.T01 | AT2G43280 | 31.452 | 124 | 76 | 2 | 23 | 137 | 1 | 124 | 1.47e-14 | 72.0 |
| MsG0780037883.01.T01 | AT4G12850 | 36.000 | 100 | 63 | 1 | 39 | 137 | 10 | 109 | 8.04e-14 | 68.2 |
| MsG0780037883.01.T01 | AT4G12850 | 36.000 | 100 | 63 | 1 | 39 | 137 | 10 | 109 | 1.55e-13 | 67.4 |
| MsG0780037883.01.T01 | AT4G12850 | 37.500 | 104 | 56 | 2 | 39 | 137 | 10 | 109 | 2.29e-13 | 68.2 |
Find 66 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAATTTGTGATGGAAAA+TGG | 0.198140 | 7:-35758015 | MsG0780037883.01.T01:CDS |
| AACGCCGATCTTTATCTAAA+TGG | 0.199214 | 7:+35757780 | None:intergenic |
| CACTTCTCGACCGGCTTCTT+TGG | 0.206955 | 7:+35757419 | None:intergenic |
| TTCATATCCTCCATATTCTT+TGG | 0.259642 | 7:+35757835 | None:intergenic |
| TATGCTAAGTCAGTTGGATT+TGG | 0.280799 | 7:-35758190 | MsG0780037883.01.T01:CDS |
| GCCACAATGGCCAAAGAATA+TGG | 0.298617 | 7:-35757845 | MsG0780037883.01.T01:CDS |
| GAGCTTTATTGATTCCTCTA+TGG | 0.299611 | 7:+35757924 | None:intergenic |
| TGATTCATGTACTGATTTAG+AGG | 0.329386 | 7:-35758287 | MsG0780037883.01.T01:CDS |
| TCAAACTCCATTCCAATGTT+AGG | 0.334083 | 7:+35758247 | None:intergenic |
| AGAGAAGACTATCAAGAGTT+TGG | 0.342771 | 7:-35757617 | MsG0780037883.01.T01:CDS |
| CGGCGTTTAGCTTTAGAATC+AGG | 0.345007 | 7:-35757764 | MsG0780037883.01.T01:CDS |
| ATGCTAAGTCAGTTGGATTT+GGG | 0.354359 | 7:-35758189 | MsG0780037883.01.T01:CDS |
| CCGGCTTCTTTGGTGCTGCT+TGG | 0.387195 | 7:+35757429 | None:intergenic |
| CTTTCATCAAATGATGCATA+TGG | 0.397301 | 7:+35757519 | None:intergenic |
| AATGGAGAACATTCTATTGA+TGG | 0.437522 | 7:-35758346 | MsG0780037883.01.T01:CDS |
| GGAAAATAATGTGCATATGC+TGG | 0.447627 | 7:+35757950 | None:intergenic |
| GAATTAATGGAAGTTGTTGA+TGG | 0.448629 | 7:-35758370 | None:intergenic |
| ATATGAAAACATTGGTTGCT+TGG | 0.449564 | 7:-35757819 | MsG0780037883.01.T01:CDS |
| ATTGAAACTTTGCACCATGC+TGG | 0.456877 | 7:-35757890 | MsG0780037883.01.T01:CDS |
| TCCATATTCTTTGGCCATTG+TGG | 0.472191 | 7:+35757844 | None:intergenic |
| ATAAGTATCGGGCCACCACT+TGG | 0.480743 | 7:-35757559 | MsG0780037883.01.T01:CDS |
| TCAATTGCATAAAAGAATCC+TGG | 0.481905 | 7:+35757692 | None:intergenic |
| TGCTTGTTCTCACTCCAGCA+TGG | 0.495070 | 7:+35757876 | None:intergenic |
| CGTGTTAAAAGAATTTGTGA+TGG | 0.498075 | 7:-35758022 | MsG0780037883.01.T01:CDS |
| ACTCATTATGCTAAGTCAGT+TGG | 0.500199 | 7:-35758196 | MsG0780037883.01.T01:CDS |
| AGGAGATGCAAATGCAATGT+TGG | 0.501317 | 7:-35757744 | MsG0780037883.01.T01:CDS |
| CATATATCACAAATAAGTAT+CGG | 0.503368 | 7:-35757571 | MsG0780037883.01.T01:CDS |
| ATGGAAGTTGTTGATGGGAA+TGG | 0.507690 | 7:-35758364 | MsG0780037883.01.T01:CDS |
| GACAAGCAAGCCTCAAGCTA+GGG | 0.511342 | 7:+35757388 | None:intergenic |
| AGTTATGCTTGCACAAGATA+CGG | 0.514711 | 7:-35758112 | MsG0780037883.01.T01:CDS |
| TATCGGGCCACCACTTGGGA+TGG | 0.516998 | 7:-35757554 | MsG0780037883.01.T01:CDS |
| ATATATCACAAATAAGTATC+GGG | 0.518571 | 7:-35757570 | MsG0780037883.01.T01:CDS |
| GTTTCAATCCACTTCTCGAC+CGG | 0.519793 | 7:+35757410 | None:intergenic |
| AGACAAGCAAGCCTCAAGCT+AGG | 0.521422 | 7:+35757387 | None:intergenic |
| AGCCACTTCGAGGAAGCGAC+TGG | 0.521852 | 7:+35757492 | None:intergenic |
| GCCACCACTTGGGATGGGAA+TGG | 0.523497 | 7:-35757548 | MsG0780037883.01.T01:CDS |
| AAATCCACGTCCTTGTTTGA+AGG | 0.523815 | 7:-35758065 | MsG0780037883.01.T01:CDS |
| TTCTTTGGTGCTGCTTGGTG+AGG | 0.532312 | 7:+35757434 | None:intergenic |
| CTCCAGTCGCTTCCTCGAAG+TGG | 0.538546 | 7:-35757494 | MsG0780037883.01.T01:CDS |
| CAAAGAAGCCGGTCGAGAAG+TGG | 0.539633 | 7:-35757418 | MsG0780037883.01.T01:CDS |
| TCCACGTCCTTGTTTGAAGG+TGG | 0.544839 | 7:-35758062 | MsG0780037883.01.T01:CDS |
| AATTAATGGAAGTTGTTGAT+GGG | 0.547188 | 7:-35758369 | None:intergenic |
| ATCGGGCCACCACTTGGGAT+GGG | 0.547511 | 7:-35757553 | MsG0780037883.01.T01:CDS |
| GAAACCATTTAGATAAAGAT+CGG | 0.550718 | 7:-35757784 | MsG0780037883.01.T01:CDS |
| GCCATTCCCATCCCAAGTGG+TGG | 0.551319 | 7:+35757547 | None:intergenic |
| TTATTGATTCCTCTATGGCA+AGG | 0.554774 | 7:+35757929 | None:intergenic |
| ATGGAGAACATTCTATTGAT+GGG | 0.556834 | 7:-35758345 | MsG0780037883.01.T01:CDS |
| TATGGAGGATATGAAAACAT+TGG | 0.557417 | 7:-35757827 | MsG0780037883.01.T01:CDS |
| CCAAGCAGCACCAAAGAAGC+CGG | 0.560219 | 7:-35757429 | MsG0780037883.01.T01:CDS |
| CCACGTCCTTGTTTGAAGGT+GGG | 0.564321 | 7:-35758061 | MsG0780037883.01.T01:CDS |
| GTGGTTGACAAGCCACTTCG+AGG | 0.565231 | 7:+35757482 | None:intergenic |
| TGCAATTGATATGGATGATG+AGG | 0.581691 | 7:-35757678 | MsG0780037883.01.T01:CDS |
| TTGTTTGAAGGTGGGATGTG+AGG | 0.585943 | 7:-35758053 | MsG0780037883.01.T01:CDS |
| CCCACCTTCAAACAAGGACG+TGG | 0.589716 | 7:+35758061 | None:intergenic |
| TGTACTGATTTAGAGGCTAA+CGG | 0.590358 | 7:-35758280 | MsG0780037883.01.T01:CDS |
| TAAGTATCGGGCCACCACTT+GGG | 0.590360 | 7:-35757558 | MsG0780037883.01.T01:CDS |
| TCACATCCCACCTTCAAACA+AGG | 0.596792 | 7:+35758055 | None:intergenic |
| TCAAGAACCTAACATTGGAA+TGG | 0.600467 | 7:-35758254 | MsG0780037883.01.T01:CDS |
| GGTGGTCAAGAACCTAACAT+TGG | 0.606305 | 7:-35758259 | MsG0780037883.01.T01:CDS |
| GAATGAAGAGCAAGTTGTCA+AGG | 0.614810 | 7:-35758323 | MsG0780037883.01.T01:CDS |
| TTTCATCAAATGATGCATAT+GGG | 0.616094 | 7:+35757520 | None:intergenic |
| TGAGCCATTCCCATCCCAAG+TGG | 0.625796 | 7:+35757544 | None:intergenic |
| TGGATTGAAACCCTAGCTTG+AGG | 0.645737 | 7:-35757398 | None:intergenic |
| GCAATTGATATGGATGATGA+GGG | 0.673224 | 7:-35757677 | MsG0780037883.01.T01:CDS |
| ACAATGGCCAAAGAATATGG+AGG | 0.745699 | 7:-35757842 | MsG0780037883.01.T01:CDS |
| ACTGATTTAGAGGCTAACGG+TGG | 0.803379 | 7:-35758277 | MsG0780037883.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATATCACAAATAAGTATC+GGG | - | Chr7:35758194-35758213 | MsG0780037883.01.T01:CDS | 20.0% |
| !! | CATATATCACAAATAAGTAT+CGG | - | Chr7:35758193-35758212 | MsG0780037883.01.T01:CDS | 20.0% |
| !!! | ATTCTTTTATGCAATTGATA+TGG | - | Chr7:35758077-35758096 | MsG0780037883.01.T01:CDS | 20.0% |
| ! | GAAACCATTTAGATAAAGAT+CGG | - | Chr7:35757980-35757999 | MsG0780037883.01.T01:CDS | 25.0% |
| ! | TTTCATCAAATGATGCATAT+GGG | + | Chr7:35758247-35758266 | None:intergenic | 25.0% |
| !! | AAAGAATTTGTGATGGAAAA+TGG | - | Chr7:35757749-35757768 | MsG0780037883.01.T01:CDS | 25.0% |
| !! | TCGCATGAAAAATGTATTTT+GGG | - | Chr7:35758110-35758129 | MsG0780037883.01.T01:CDS | 25.0% |
| AATGGAGAACATTCTATTGA+TGG | - | Chr7:35757418-35757437 | MsG0780037883.01.T01:CDS | 30.0% | |
| ATGGAGAACATTCTATTGAT+GGG | - | Chr7:35757419-35757438 | MsG0780037883.01.T01:CDS | 30.0% | |
| CGTGTTAAAAGAATTTGTGA+TGG | - | Chr7:35757742-35757761 | MsG0780037883.01.T01:CDS | 30.0% | |
| CTTTCATCAAATGATGCATA+TGG | + | Chr7:35758248-35758267 | None:intergenic | 30.0% | |
| TATGGAGGATATGAAAACAT+TGG | - | Chr7:35757937-35757956 | MsG0780037883.01.T01:CDS | 30.0% | |
| TCAATTGCATAAAAGAATCC+TGG | + | Chr7:35758075-35758094 | None:intergenic | 30.0% | |
| TTCATATCCTCCATATTCTT+TGG | + | Chr7:35757932-35757951 | None:intergenic | 30.0% | |
| ! | AAGCAAAAATTTTGCCACAA+TGG | - | Chr7:35757906-35757925 | MsG0780037883.01.T01:CDS | 30.0% |
| ! | ATATGAAAACATTGGTTGCT+TGG | - | Chr7:35757945-35757964 | MsG0780037883.01.T01:CDS | 30.0% |
| ! | GTCGCATGAAAAATGTATTT+TGG | - | Chr7:35758109-35758128 | MsG0780037883.01.T01:CDS | 30.0% |
| !! | CATGAAAAATGTATTTTGGG+TGG | - | Chr7:35758113-35758132 | MsG0780037883.01.T01:CDS | 30.0% |
| !! | TGATTCATGTACTGATTTAG+AGG | - | Chr7:35757477-35757496 | MsG0780037883.01.T01:CDS | 30.0% |
| AACGCCGATCTTTATCTAAA+TGG | + | Chr7:35757987-35758006 | None:intergenic | 35.0% | |
| ACTCATTATGCTAAGTCAGT+TGG | - | Chr7:35757568-35757587 | MsG0780037883.01.T01:CDS | 35.0% | |
| AGAGAAGACTATCAAGAGTT+TGG | - | Chr7:35758147-35758166 | MsG0780037883.01.T01:CDS | 35.0% | |
| AGTTATGCTTGCACAAGATA+CGG | - | Chr7:35757652-35757671 | MsG0780037883.01.T01:CDS | 35.0% | |
| ATGCTAAGTCAGTTGGATTT+GGG | - | Chr7:35757575-35757594 | MsG0780037883.01.T01:CDS | 35.0% | |
| CTCATGCAAGAAAAAAATCC+AGG | - | Chr7:35758054-35758073 | MsG0780037883.01.T01:CDS | 35.0% | |
| GCAATTGATATGGATGATGA+GGG | - | Chr7:35758087-35758106 | MsG0780037883.01.T01:CDS | 35.0% | |
| GGAAAATAATGTGCATATGC+TGG | + | Chr7:35757817-35757836 | None:intergenic | 35.0% | |
| TATGCTAAGTCAGTTGGATT+TGG | - | Chr7:35757574-35757593 | MsG0780037883.01.T01:CDS | 35.0% | |
| TCAAACTCCATTCCAATGTT+AGG | + | Chr7:35757520-35757539 | None:intergenic | 35.0% | |
| TCAAGAACCTAACATTGGAA+TGG | - | Chr7:35757510-35757529 | MsG0780037883.01.T01:CDS | 35.0% | |
| TGCAATTGATATGGATGATG+AGG | - | Chr7:35758086-35758105 | MsG0780037883.01.T01:CDS | 35.0% | |
| ! | CATTATTTTCCTTGCCATAG+AGG | - | Chr7:35757826-35757845 | MsG0780037883.01.T01:CDS | 35.0% |
| !! | GAGCTTTATTGATTCCTCTA+TGG | + | Chr7:35757843-35757862 | None:intergenic | 35.0% |
| !! | TGTACTGATTTAGAGGCTAA+CGG | - | Chr7:35757484-35757503 | MsG0780037883.01.T01:CDS | 35.0% |
| !! | TTATTGATTCCTCTATGGCA+AGG | + | Chr7:35757838-35757857 | None:intergenic | 35.0% |
| ACAATGGCCAAAGAATATGG+AGG | - | Chr7:35757922-35757941 | MsG0780037883.01.T01:CDS | 40.0% | |
| AGGAGATGCAAATGCAATGT+TGG | - | Chr7:35758020-35758039 | MsG0780037883.01.T01:CDS | 40.0% | |
| ATTGAAACTTTGCACCATGC+TGG | - | Chr7:35757874-35757893 | MsG0780037883.01.T01:CDS | 40.0% | |
| CTGGAAAAAGTTCGTGATTG+TGG | + | Chr7:35757798-35757817 | None:intergenic | 40.0% | |
| GAATGAAGAGCAAGTTGTCA+AGG | - | Chr7:35757441-35757460 | MsG0780037883.01.T01:CDS | 40.0% | |
| TCCATATTCTTTGGCCATTG+TGG | + | Chr7:35757923-35757942 | None:intergenic | 40.0% | |
| ! | AAATCCACGTCCTTGTTTGA+AGG | - | Chr7:35757699-35757718 | MsG0780037883.01.T01:CDS | 40.0% |
| ! | ATGGAAGTTGTTGATGGGAA+TGG | - | Chr7:35757400-35757419 | MsG0780037883.01.T01:CDS | 40.0% |
| !! | GTATTTTGGGTGGATGCAAA+AGG | - | Chr7:35758123-35758142 | MsG0780037883.01.T01:CDS | 40.0% |
| GCCACAATGGCCAAAGAATA+TGG | - | Chr7:35757919-35757938 | MsG0780037883.01.T01:CDS | 45.0% | |
| GGTGGTCAAGAACCTAACAT+TGG | - | Chr7:35757505-35757524 | MsG0780037883.01.T01:CDS | 45.0% | |
| GTTTCAATCCACTTCTCGAC+CGG | + | Chr7:35758357-35758376 | None:intergenic | 45.0% | |
| TCACATCCCACCTTCAAACA+AGG | + | Chr7:35757712-35757731 | None:intergenic | 45.0% | |
| TTGTTTGAAGGTGGGATGTG+AGG | - | Chr7:35757711-35757730 | MsG0780037883.01.T01:CDS | 45.0% | |
| ! | ACTGATTTAGAGGCTAACGG+TGG | - | Chr7:35757487-35757506 | MsG0780037883.01.T01:CDS | 45.0% |
| ! | AGGACGTGGATTTTGTGAAC+TGG | + | Chr7:35757692-35757711 | None:intergenic | 45.0% |
| !! | CGGCGTTTAGCTTTAGAATC+AGG | - | Chr7:35758000-35758019 | MsG0780037883.01.T01:CDS | 45.0% |
| !!! | AGGAGCATTGTTTTGAGGGA+GGG | + | Chr7:35758313-35758332 | None:intergenic | 45.0% |
| !!! | GGTGAGGAGCATTGTTTTGA+GGG | + | Chr7:35758317-35758336 | None:intergenic | 45.0% |
| !!! | TGGTGAGGAGCATTGTTTTG+AGG | + | Chr7:35758318-35758337 | None:intergenic | 45.0% |
| TGCTTGTTCTCACTCCAGCA+TGG | + | Chr7:35757891-35757910 | None:intergenic | 50.0% | |
| ! | CCACGTCCTTGTTTGAAGGT+GGG | - | Chr7:35757703-35757722 | MsG0780037883.01.T01:CDS | 50.0% |
| ! | TCCACGTCCTTGTTTGAAGG+TGG | - | Chr7:35757702-35757721 | MsG0780037883.01.T01:CDS | 50.0% |
| !! | ATAAGTATCGGGCCACCACT+TGG | - | Chr7:35758205-35758224 | MsG0780037883.01.T01:CDS | 50.0% |
| !! | TAAGTATCGGGCCACCACTT+GGG | - | Chr7:35758206-35758225 | MsG0780037883.01.T01:CDS | 50.0% |
| !! | TTCTTTGGTGCTGCTTGGTG+AGG | + | Chr7:35758333-35758352 | None:intergenic | 50.0% |
| !!! | GAGGAGCATTGTTTTGAGGG+AGG | + | Chr7:35758314-35758333 | None:intergenic | 50.0% |
| CAAAGAAGCCGGTCGAGAAG+TGG | - | Chr7:35758346-35758365 | MsG0780037883.01.T01:CDS | 55.0% | |
| CCAAGCAGCACCAAAGAAGC+CGG | - | Chr7:35758335-35758354 | MsG0780037883.01.T01:CDS | 55.0% | |
| CCCACCTTCAAACAAGGACG+TGG | + | Chr7:35757706-35757725 | None:intergenic | 55.0% | |
| GTGGTTGACAAGCCACTTCG+AGG | + | Chr7:35758285-35758304 | None:intergenic | 55.0% | |
| TGAGCCATTCCCATCCCAAG+TGG | + | Chr7:35758223-35758242 | None:intergenic | 55.0% | |
| ! | CACTTCTCGACCGGCTTCTT+TGG | + | Chr7:35758348-35758367 | None:intergenic | 55.0% |
| !!! | GTTTTGAGGGAGGGAAAGCG+TGG | + | Chr7:35758304-35758323 | None:intergenic | 55.0% |
| AGCCACTTCGAGGAAGCGAC+TGG | + | Chr7:35758275-35758294 | None:intergenic | 60.0% | |
| ATCGGGCCACCACTTGGGAT+GGG | - | Chr7:35758211-35758230 | MsG0780037883.01.T01:CDS | 60.0% | |
| CTCCAGTCGCTTCCTCGAAG+TGG | - | Chr7:35758270-35758289 | MsG0780037883.01.T01:CDS | 60.0% | |
| GCCACCACTTGGGATGGGAA+TGG | - | Chr7:35758216-35758235 | MsG0780037883.01.T01:CDS | 60.0% | |
| GCCATTCCCATCCCAAGTGG+TGG | + | Chr7:35758220-35758239 | None:intergenic | 60.0% | |
| TATCGGGCCACCACTTGGGA+TGG | - | Chr7:35758210-35758229 | MsG0780037883.01.T01:CDS | 60.0% | |
| !! | CCGGCTTCTTTGGTGCTGCT+TGG | + | Chr7:35758338-35758357 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 35757400 | 35758386 | 35757400 | ID=MsG0780037883.01;Name=MsG0780037883.01 |
| Chr7 | mRNA | 35757400 | 35758386 | 35757400 | ID=MsG0780037883.01.T01;Parent=MsG0780037883.01;Name=MsG0780037883.01.T01;_AED=0.39;_eAED=0.39;_QI=0|-1|0|1|-1|1|1|0|328 |
| Chr7 | exon | 35757400 | 35758386 | 35757400 | ID=MsG0780037883.01.T01:exon:16439;Parent=MsG0780037883.01.T01 |
| Chr7 | CDS | 35757400 | 35758386 | 35757400 | ID=MsG0780037883.01.T01:cds;Parent=MsG0780037883.01.T01 |
| Gene Sequence |
| Protein sequence |
