Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038266.01.T01 | GAU32754.1 | 67.816 | 174 | 36 | 1 | 1 | 154 | 758 | 931 | 2.30E-74 | 251 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038266.01.T01 | Q9SZL8 | 27.439 | 164 | 96 | 3 | 4 | 144 | 70 | 233 | 1.59E-13 | 70.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038266.01.T01 | A0A2Z6MJF1 | 67.816 | 174 | 36 | 1 | 1 | 154 | 758 | 931 | 1.10e-74 | 251 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0780038266.01.T01 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038266.01.T01 | MTR_4g102720 | 48.193 | 166 | 35 | 2 | 1 | 148 | 1 | 133 | 3.41e-47 | 153 |
| MsG0780038266.01.T01 | MTR_7g074790 | 36.025 | 161 | 66 | 4 | 3 | 144 | 6 | 148 | 3.97e-23 | 91.3 |
| MsG0780038266.01.T01 | MTR_0402s0040 | 34.375 | 160 | 83 | 3 | 6 | 144 | 25 | 183 | 5.10e-21 | 88.6 |
| MsG0780038266.01.T01 | MTR_7g061980 | 37.168 | 113 | 52 | 2 | 4 | 97 | 7 | 119 | 8.71e-19 | 77.4 |
| MsG0780038266.01.T01 | MTR_7g113010 | 36.283 | 113 | 65 | 2 | 7 | 112 | 457 | 569 | 2.45e-15 | 72.4 |
| MsG0780038266.01.T01 | MTR_5g015480 | 35.714 | 126 | 60 | 2 | 6 | 110 | 55 | 180 | 1.14e-14 | 69.3 |
| MsG0780038266.01.T01 | MTR_5g039760 | 30.435 | 161 | 68 | 4 | 4 | 144 | 7 | 143 | 1.39e-14 | 68.2 |
| MsG0780038266.01.T01 | MTR_2g070590 | 34.615 | 104 | 47 | 2 | 6 | 88 | 16 | 119 | 3.00e-12 | 60.5 |
| MsG0780038266.01.T01 | MTR_2g098920 | 34.314 | 102 | 46 | 3 | 7 | 88 | 80 | 180 | 8.87e-12 | 61.6 |
| MsG0780038266.01.T01 | MTR_1g008290 | 29.474 | 95 | 64 | 2 | 46 | 140 | 9 | 100 | 5.87e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038266.01.T01 | AT4G38180 | 27.439 | 164 | 96 | 3 | 4 | 144 | 70 | 233 | 1.62e-14 | 70.1 |
Find 18 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGATGATGCTTGGAAGTTT+TGG | 0.271793 | 7:-43101797 | MsG0780038266.01.T01:CDS |
| TTCGTTTGATTTGATGAGTA+AGG | 0.372630 | 7:-43101419 | MsG0780038266.01.T01:CDS |
| TTAGTTCGAGTCTCTGCTCT+TGG | 0.438175 | 7:+43101628 | None:intergenic |
| GGTACAGAATGGAAGAAGAT+TGG | 0.456380 | 7:-43101851 | None:intergenic |
| AGGTTTGTGTGTTGCAAAGA+AGG | 0.468477 | 7:-43101691 | MsG0780038266.01.T01:CDS |
| AGTAGGTGGCAGAACCAACT+TGG | 0.472462 | 7:-43101395 | MsG0780038266.01.T01:CDS |
| GTTGATTATGGTGGAAAAGT+TGG | 0.473224 | 7:-43101774 | MsG0780038266.01.T01:CDS |
| TTATGGTGGAAAAGTTGGTT+TGG | 0.477774 | 7:-43101769 | MsG0780038266.01.T01:CDS |
| TTGATAACTTTGATGATGCT+TGG | 0.486016 | 7:-43101806 | MsG0780038266.01.T01:CDS |
| TTTCTTGTGAGTGTAACATA+TGG | 0.502845 | 7:+43101533 | None:intergenic |
| GATTTGATGAGTAAGGAAGT+AGG | 0.506286 | 7:-43101412 | MsG0780038266.01.T01:CDS |
| GAAGATTGGATGCCAAGGAT+TGG | 0.520207 | 7:-43101837 | MsG0780038266.01.T01:CDS |
| TGGAAGAAGATTGGATGCCA+AGG | 0.524415 | 7:-43101842 | MsG0780038266.01.T01:CDS |
| ACTAACTGTCAAGCAACGAT+TGG | 0.586736 | 7:-43101610 | MsG0780038266.01.T01:CDS |
| AAGGCGTATGCATCCCAAGT+TGG | 0.600278 | 7:+43101381 | None:intergenic |
| ATTGATCTAGCAGATGATGC+CGG | 0.612239 | 7:-43101457 | MsG0780038266.01.T01:CDS |
| GTAGGTGGCAGAACCAACTT+GGG | 0.642938 | 7:-43101394 | MsG0780038266.01.T01:CDS |
| TTGATGAGTAAGGAAGTAGG+TGG | 0.658185 | 7:-43101409 | MsG0780038266.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAAAAATATGGGTGAAAAA+TGG | - | Chr7:43101597-43101616 | MsG0780038266.01.T01:CDS | 20.0% |
| ! | AAAATTATCTCCGAAAAAGA+AGG | - | Chr7:43101830-43101849 | MsG0780038266.01.T01:CDS | 25.0% |
| !!! | AACGATTGGTCTTAAAAATA+TGG | - | Chr7:43101586-43101605 | MsG0780038266.01.T01:CDS | 25.0% |
| !!! | ACGATTGGTCTTAAAAATAT+GGG | - | Chr7:43101587-43101606 | MsG0780038266.01.T01:CDS | 25.0% |
| AAATATGGGTGAAAAATGGA+CGG | - | Chr7:43101601-43101620 | MsG0780038266.01.T01:CDS | 30.0% | |
| TTTCTTGTGAGTGTAACATA+TGG | + | Chr7:43101652-43101671 | None:intergenic | 30.0% | |
| ! | AACTTCTTTGCCTTCTTTTT+CGG | + | Chr7:43101843-43101862 | None:intergenic | 30.0% |
| ! | GTTTTATAATCTCGTTTGTC+TGG | + | Chr7:43101527-43101546 | None:intergenic | 30.0% |
| !! | TCTGAAACTTTTTGTTGAGA+TGG | + | Chr7:43101689-43101708 | None:intergenic | 30.0% |
| !! | TTCGTTTGATTTGATGAGTA+AGG | - | Chr7:43101763-43101782 | MsG0780038266.01.T01:intron | 30.0% |
| !! | TTGATAACTTTGATGATGCT+TGG | - | Chr7:43101376-43101395 | MsG0780038266.01.T01:CDS | 30.0% |
| !!! | CGGAGATAATTTTTTGATCA+AGG | + | Chr7:43101823-43101842 | None:intergenic | 30.0% |
| ATACGTACACAAGAGCAAAA+AGG | - | Chr7:43101448-43101467 | MsG0780038266.01.T01:CDS | 35.0% | |
| GATTTGATGAGTAAGGAAGT+AGG | - | Chr7:43101770-43101789 | MsG0780038266.01.T01:CDS | 35.0% | |
| TCAAAAATCATGCCAATCCT+TGG | + | Chr7:43101360-43101379 | None:intergenic | 35.0% | |
| ! | GTTGATTATGGTGGAAAAGT+TGG | - | Chr7:43101408-43101427 | MsG0780038266.01.T01:CDS | 35.0% |
| ! | TTATGGTGGAAAAGTTGGTT+TGG | - | Chr7:43101413-43101432 | MsG0780038266.01.T01:CDS | 35.0% |
| !! | TGATGATGCTTGGAAGTTTT+GGG | - | Chr7:43101386-43101405 | MsG0780038266.01.T01:CDS | 35.0% |
| !!! | AAGTTTTGGGTTGATTATGG+TGG | - | Chr7:43101399-43101418 | MsG0780038266.01.T01:CDS | 35.0% |
| !!! | TGGAAGTTTTGGGTTGATTA+TGG | - | Chr7:43101396-43101415 | MsG0780038266.01.T01:CDS | 35.0% |
| !!! | TTGATGATGCTTGGAAGTTT+TGG | - | Chr7:43101385-43101404 | MsG0780038266.01.T01:CDS | 35.0% |
| ACTAACTGTCAAGCAACGAT+TGG | - | Chr7:43101572-43101591 | MsG0780038266.01.T01:CDS | 40.0% | |
| AGGTTTGTGTGTTGCAAAGA+AGG | - | Chr7:43101491-43101510 | MsG0780038266.01.T01:CDS | 40.0% | |
| ATTGATCTAGCAGATGATGC+CGG | - | Chr7:43101725-43101744 | MsG0780038266.01.T01:intron | 40.0% | |
| GTACACAAGAGCAAAAAGGA+TGG | - | Chr7:43101452-43101471 | MsG0780038266.01.T01:CDS | 40.0% | |
| TTGATGAGTAAGGAAGTAGG+TGG | - | Chr7:43101773-43101792 | MsG0780038266.01.T01:CDS | 40.0% | |
| ! | CGAACTCTTTTGTTGAAGAC+CGG | + | Chr7:43101747-43101766 | None:intergenic | 40.0% |
| CACACAAACCTGCAAGAAGT+AGG | + | Chr7:43101482-43101501 | None:intergenic | 45.0% | |
| GAAGATTGGATGCCAAGGAT+TGG | - | Chr7:43101345-43101364 | MsG0780038266.01.T01:CDS | 45.0% | |
| TGGAAGAAGATTGGATGCCA+AGG | - | Chr7:43101340-43101359 | MsG0780038266.01.T01:CDS | 45.0% | |
| TTAGTTCGAGTCTCTGCTCT+TGG | + | Chr7:43101557-43101576 | None:intergenic | 45.0% | |
| AAGGCGTATGCATCCCAAGT+TGG | + | Chr7:43101804-43101823 | None:intergenic | 50.0% | |
| AGTAGGTGGCAGAACCAACT+TGG | - | Chr7:43101787-43101806 | MsG0780038266.01.T01:CDS | 50.0% | |
| ATGGCTCACCTACTTCTTGC+AGG | - | Chr7:43101471-43101490 | MsG0780038266.01.T01:CDS | 50.0% | |
| GTAGGTGGCAGAACCAACTT+GGG | - | Chr7:43101788-43101807 | MsG0780038266.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 43101339 | 43101865 | 43101339 | ID=MsG0780038266.01;Name=MsG0780038266.01 |
| Chr7 | mRNA | 43101339 | 43101865 | 43101339 | ID=MsG0780038266.01.T01;Parent=MsG0780038266.01;Name=MsG0780038266.01.T01;_AED=0.47;_eAED=0.48;_QI=0|0|0|1|1|1|2|0|156 |
| Chr7 | exon | 43101768 | 43101865 | 43101768 | ID=MsG0780038266.01.T01:exon:8373;Parent=MsG0780038266.01.T01 |
| Chr7 | exon | 43101339 | 43101711 | 43101339 | ID=MsG0780038266.01.T01:exon:8372;Parent=MsG0780038266.01.T01 |
| Chr7 | CDS | 43101768 | 43101865 | 43101768 | ID=MsG0780038266.01.T01:cds;Parent=MsG0780038266.01.T01 |
| Chr7 | CDS | 43101339 | 43101711 | 43101339 | ID=MsG0780038266.01.T01:cds;Parent=MsG0780038266.01.T01 |
| Gene Sequence |
| Protein sequence |
