Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038387.01.T01 | XP_013468829.1 | 91.824 | 159 | 13 | 0 | 1 | 159 | 1 | 159 | 1.14E-93 | 278 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038387.01.T01 | Q9FJK3 | 46.154 | 156 | 83 | 1 | 1 | 155 | 1 | 156 | 5.39E-33 | 122 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038387.01.T01 | A0A072VMQ1 | 91.824 | 159 | 13 | 0 | 1 | 159 | 1 | 159 | 5.44e-94 | 278 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0780038387.01.T01 | TF | MADS-M-type |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038387.01.T01 | MTR_1g077320 | 91.824 | 159 | 13 | 0 | 1 | 159 | 1 | 159 | 1.38e-97 | 278 |
| MsG0780038387.01.T01 | MTR_7g011950 | 88.679 | 159 | 18 | 0 | 1 | 159 | 1 | 159 | 3.25e-94 | 270 |
| MsG0780038387.01.T01 | MTR_4g028720 | 86.792 | 159 | 21 | 0 | 1 | 159 | 1 | 159 | 3.36e-94 | 270 |
| MsG0780038387.01.T01 | MTR_1g077390 | 71.975 | 157 | 44 | 0 | 1 | 157 | 1 | 157 | 2.21e-79 | 232 |
| MsG0780038387.01.T01 | MTR_1g077300 | 74.843 | 159 | 38 | 1 | 1 | 159 | 1 | 157 | 7.00e-75 | 221 |
| MsG0780038387.01.T01 | MTR_5g047580 | 68.590 | 156 | 49 | 0 | 1 | 156 | 1 | 156 | 7.30e-74 | 218 |
| MsG0780038387.01.T01 | MTR_1g084950 | 73.077 | 156 | 42 | 0 | 1 | 156 | 1 | 156 | 8.59e-73 | 216 |
| MsG0780038387.01.T01 | MTR_1g090697 | 57.419 | 155 | 66 | 0 | 1 | 155 | 1 | 155 | 8.51e-59 | 180 |
| MsG0780038387.01.T01 | MTR_1g090710 | 55.769 | 156 | 68 | 1 | 1 | 155 | 1 | 156 | 7.70e-57 | 175 |
| MsG0780038387.01.T01 | MTR_5g047560 | 57.692 | 156 | 50 | 1 | 1 | 156 | 1 | 140 | 4.92e-56 | 172 |
| MsG0780038387.01.T01 | MTR_1g090783 | 54.902 | 153 | 69 | 0 | 3 | 155 | 2 | 154 | 2.14e-55 | 171 |
| MsG0780038387.01.T01 | MTR_3g065100 | 49.682 | 157 | 78 | 1 | 1 | 156 | 1 | 157 | 1.14e-38 | 132 |
| MsG0780038387.01.T01 | MTR_5g075380 | 39.744 | 156 | 93 | 1 | 1 | 155 | 1 | 156 | 3.54e-38 | 128 |
| MsG0780038387.01.T01 | MTR_3g466980 | 43.137 | 153 | 86 | 1 | 1 | 152 | 1 | 153 | 1.05e-36 | 126 |
| MsG0780038387.01.T01 | MTR_4g032620 | 37.419 | 155 | 95 | 2 | 1 | 153 | 1 | 155 | 3.72e-35 | 122 |
| MsG0780038387.01.T01 | MTR_4g063790 | 38.994 | 159 | 95 | 2 | 1 | 157 | 1 | 159 | 7.95e-34 | 117 |
| MsG0780038387.01.T01 | MTR_3g466830 | 42.038 | 157 | 90 | 1 | 3 | 158 | 2 | 158 | 4.42e-33 | 115 |
| MsG0780038387.01.T01 | MTR_4g031910 | 43.333 | 150 | 83 | 2 | 1 | 148 | 1 | 150 | 6.48e-32 | 114 |
| MsG0780038387.01.T01 | MTR_3g031240 | 36.478 | 159 | 99 | 2 | 1 | 157 | 1 | 159 | 3.30e-31 | 111 |
| MsG0780038387.01.T01 | MTR_4g032260 | 43.125 | 160 | 89 | 2 | 1 | 158 | 1 | 160 | 2.90e-30 | 107 |
| MsG0780038387.01.T01 | MTR_3g466890 | 40.260 | 154 | 91 | 1 | 3 | 155 | 2 | 155 | 2.98e-30 | 107 |
| MsG0780038387.01.T01 | MTR_2g016210 | 39.623 | 159 | 94 | 2 | 1 | 157 | 1 | 159 | 1.77e-29 | 108 |
| MsG0780038387.01.T01 | MTR_8g036130 | 37.107 | 159 | 96 | 2 | 1 | 155 | 1 | 159 | 5.11e-29 | 107 |
| MsG0780038387.01.T01 | MTR_3g031100 | 36.709 | 158 | 96 | 3 | 1 | 155 | 1 | 157 | 1.53e-27 | 103 |
| MsG0780038387.01.T01 | MTR_4g032290 | 43.333 | 150 | 83 | 2 | 1 | 148 | 1 | 150 | 6.88e-27 | 101 |
| MsG0780038387.01.T01 | MTR_3g467080 | 33.333 | 159 | 104 | 2 | 1 | 157 | 1 | 159 | 1.22e-26 | 98.6 |
| MsG0780038387.01.T01 | MTR_2g035610 | 41.880 | 117 | 67 | 1 | 1 | 116 | 1 | 117 | 1.69e-24 | 92.0 |
| MsG0780038387.01.T01 | MTR_2g035580 | 32.903 | 155 | 103 | 1 | 1 | 154 | 1 | 155 | 3.44e-22 | 87.0 |
| MsG0780038387.01.T01 | MTR_3g465410 | 35.115 | 131 | 83 | 2 | 29 | 157 | 1 | 131 | 1.53e-21 | 85.1 |
| MsG0780038387.01.T01 | MTR_4g028800 | 46.575 | 73 | 37 | 1 | 1 | 73 | 1 | 71 | 2.85e-19 | 77.4 |
| MsG0780038387.01.T01 | MTR_3g466930 | 36.000 | 125 | 79 | 1 | 32 | 155 | 2 | 126 | 1.32e-18 | 77.4 |
| MsG0780038387.01.T01 | MTR_4g019670 | 33.553 | 152 | 94 | 3 | 1 | 148 | 1 | 149 | 2.85e-18 | 80.9 |
| MsG0780038387.01.T01 | MTR_3g466900 | 37.302 | 126 | 76 | 2 | 32 | 155 | 2 | 126 | 6.19e-18 | 75.5 |
| MsG0780038387.01.T01 | MTR_7g106510 | 36.111 | 108 | 68 | 1 | 3 | 109 | 6 | 113 | 5.28e-17 | 77.4 |
| MsG0780038387.01.T01 | MTR_7g055800 | 32.456 | 114 | 50 | 2 | 1 | 114 | 1 | 87 | 1.53e-13 | 63.2 |
| MsG0780038387.01.T01 | MTR_3g093900 | 27.632 | 152 | 105 | 2 | 3 | 151 | 6 | 155 | 6.02e-13 | 65.9 |
| MsG0780038387.01.T01 | MTR_7g055790 | 37.037 | 108 | 51 | 2 | 1 | 108 | 59 | 149 | 8.66e-12 | 60.1 |
| MsG0780038387.01.T01 | MTR_1g033370 | 40.426 | 94 | 55 | 1 | 64 | 156 | 28 | 121 | 1.21e-11 | 58.9 |
| MsG0780038387.01.T01 | MTR_1g033930 | 37.500 | 96 | 59 | 1 | 64 | 158 | 22 | 117 | 1.46e-11 | 58.5 |
| MsG0780038387.01.T01 | MTR_7g055940 | 33.333 | 105 | 43 | 3 | 1 | 105 | 1 | 78 | 2.37e-11 | 57.0 |
| MsG0780038387.01.T01 | MTR_6g005450 | 29.464 | 112 | 73 | 3 | 1 | 106 | 1 | 112 | 5.27e-11 | 60.1 |
| MsG0780038387.01.T01 | MTR_1g033790 | 38.542 | 96 | 58 | 1 | 64 | 158 | 22 | 117 | 5.93e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038387.01.T01 | AT5G48670 | 46.154 | 156 | 83 | 1 | 1 | 155 | 1 | 156 | 5.50e-34 | 122 |
| MsG0780038387.01.T01 | AT3G05860 | 39.597 | 149 | 89 | 1 | 1 | 148 | 1 | 149 | 3.40e-31 | 111 |
| MsG0780038387.01.T01 | AT3G05860 | 39.597 | 149 | 89 | 1 | 1 | 148 | 1 | 149 | 1.95e-30 | 110 |
| MsG0780038387.01.T01 | AT3G05860 | 39.597 | 149 | 89 | 1 | 1 | 148 | 1 | 149 | 2.49e-30 | 110 |
| MsG0780038387.01.T01 | AT5G26630 | 39.375 | 160 | 96 | 1 | 1 | 159 | 1 | 160 | 1.11e-29 | 108 |
| MsG0780038387.01.T01 | AT1G65300 | 36.735 | 147 | 92 | 1 | 3 | 148 | 2 | 148 | 6.64e-28 | 105 |
| MsG0780038387.01.T01 | AT1G65330 | 37.415 | 147 | 91 | 1 | 3 | 148 | 2 | 148 | 2.24e-27 | 103 |
| MsG0780038387.01.T01 | AT5G26650 | 35.948 | 153 | 97 | 1 | 4 | 155 | 2 | 154 | 8.35e-25 | 98.2 |
| MsG0780038387.01.T01 | AT5G27960 | 44.860 | 107 | 58 | 1 | 4 | 109 | 2 | 108 | 2.99e-24 | 96.3 |
| MsG0780038387.01.T01 | AT1G22590 | 29.630 | 162 | 98 | 3 | 1 | 155 | 1 | 153 | 2.26e-21 | 85.1 |
| MsG0780038387.01.T01 | AT5G27810 | 43.750 | 96 | 53 | 1 | 29 | 123 | 1 | 96 | 2.44e-20 | 81.3 |
| MsG0780038387.01.T01 | AT1G31630 | 29.032 | 155 | 108 | 2 | 3 | 156 | 2 | 155 | 1.41e-19 | 84.0 |
| MsG0780038387.01.T01 | AT2G28700 | 34.532 | 139 | 88 | 2 | 1 | 138 | 1 | 137 | 6.66e-19 | 82.0 |
| MsG0780038387.01.T01 | AT1G31640 | 39.640 | 111 | 65 | 2 | 3 | 112 | 2 | 111 | 1.58e-18 | 81.6 |
| MsG0780038387.01.T01 | AT5G26580 | 37.615 | 109 | 55 | 1 | 1 | 109 | 1 | 96 | 1.21e-16 | 75.9 |
| MsG0780038387.01.T01 | AT2G40210 | 38.835 | 103 | 58 | 2 | 1 | 100 | 1 | 101 | 2.30e-13 | 66.6 |
| MsG0780038387.01.T01 | AT5G58890 | 34.234 | 111 | 65 | 3 | 1 | 104 | 1 | 110 | 4.94e-13 | 65.5 |
| MsG0780038387.01.T01 | AT5G06500 | 36.792 | 106 | 63 | 2 | 1 | 102 | 1 | 106 | 1.62e-12 | 63.5 |
Find 27 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTGCAAAAGGAAACTTTCT+TGG | 0.253590 | 7:+45618254 | None:intergenic |
| AACAGAGGTGTGGCCAAATC+TGG | 0.333286 | 7:-45618344 | MsG0780038387.01.T01:CDS |
| TGTTGAATCATTGGAGATAA+AGG | 0.341938 | 7:+45618480 | None:intergenic |
| CTTGTCTTTGCTTTCTCAGT+TGG | 0.347479 | 7:+45618212 | None:intergenic |
| AGCCTTTCTTGTTGAATCAT+TGG | 0.353862 | 7:+45618471 | None:intergenic |
| CTCCAATGATTCAACAAGAA+AGG | 0.407412 | 7:-45618473 | MsG0780038387.01.T01:CDS |
| AAATCTGGAGGAAGCAAAAC+AGG | 0.418438 | 7:-45618329 | MsG0780038387.01.T01:CDS |
| CTTTAGTAATCTGTTGCAAA+AGG | 0.444882 | 7:+45618242 | None:intergenic |
| TCTTTCTGATTTGAAAGACT+TGG | 0.461416 | 7:-45618116 | MsG0780038387.01.T01:CDS |
| GAAGAAAAGTATCATAAAGA+AGG | 0.475254 | 7:-45618434 | MsG0780038387.01.T01:CDS |
| TCAGAAAGAGTGATATCATC+TGG | 0.482942 | 7:+45618130 | None:intergenic |
| TTTCACCTTCTTCCTAGTCA+TGG | 0.490416 | 7:+45618507 | None:intergenic |
| CAGAACTCATATATGAAAGA+TGG | 0.506115 | 7:-45618292 | MsG0780038387.01.T01:CDS |
| AGCTGAACATTCGTATGAGC+TGG | 0.525005 | 7:-45618174 | MsG0780038387.01.T01:CDS |
| AGAGAACTCACTATTCTTTG+TGG | 0.526508 | 7:-45618409 | MsG0780038387.01.T01:CDS |
| TGCAACAGATTACTAAAGCT+AGG | 0.531119 | 7:-45618237 | MsG0780038387.01.T01:CDS |
| GCAACAGATTACTAAAGCTA+GGG | 0.532673 | 7:-45618236 | MsG0780038387.01.T01:CDS |
| GAAGGAAATTGATGACAAGA+TGG | 0.550366 | 7:-45618071 | MsG0780038387.01.T01:CDS |
| CGAAGCCATGACTAGGAAGA+AGG | 0.551367 | 7:-45618512 | None:intergenic |
| AGGATCGGAAATTATAGCAC+AGG | 0.559456 | 7:+45618378 | None:intergenic |
| GAAAGGCTTCCTACAAGAAG+AGG | 0.563850 | 7:-45618456 | MsG0780038387.01.T01:CDS |
| TCGGAAATTATAGCACAGGC+TGG | 0.570119 | 7:+45618382 | None:intergenic |
| TACATTGCGAAGCCATGACT+AGG | 0.618148 | 7:-45618519 | None:intergenic |
| TTGATTCTAAAACAGAGGTG+TGG | 0.633348 | 7:-45618354 | MsG0780038387.01.T01:CDS |
| AAGCAAAACAGGTGATTGAG+AGG | 0.635256 | 7:-45618318 | MsG0780038387.01.T01:CDS |
| AGAGGTGTGGCCAAATCTGG+AGG | 0.647190 | 7:-45618341 | MsG0780038387.01.T01:CDS |
| CTGGTACCTGCAAAACAACA+CGG | 0.672567 | 7:-45618155 | MsG0780038387.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTAATTGCAAAAAGTATGA+AGG | - | Chr7:45618464-45618483 | MsG0780038387.01.T01:CDS | 25.0% |
| !!! | GAAGAAAAGTATCATAAAGA+AGG | - | Chr7:45618119-45618138 | MsG0780038387.01.T01:CDS | 25.0% |
| !!! | TGTTTTAGAATCAAAAGGAT+CGG | + | Chr7:45618193-45618212 | None:intergenic | 25.0% |
| CAGAACTCATATATGAAAGA+TGG | - | Chr7:45618261-45618280 | MsG0780038387.01.T01:CDS | 30.0% | |
| CTTTAGTAATCTGTTGCAAA+AGG | + | Chr7:45618314-45618333 | None:intergenic | 30.0% | |
| TGTTGAATCATTGGAGATAA+AGG | + | Chr7:45618076-45618095 | None:intergenic | 30.0% | |
| ! | TCTTTCTGATTTGAAAGACT+TGG | - | Chr7:45618437-45618456 | MsG0780038387.01.T01:CDS | 30.0% |
| !!! | ACCTCTGTTTTAGAATCAAA+AGG | + | Chr7:45618198-45618217 | None:intergenic | 30.0% |
| !!! | TCCTTTTGATTCTAAAACAG+AGG | - | Chr7:45618194-45618213 | MsG0780038387.01.T01:CDS | 30.0% |
| AGAGAACTCACTATTCTTTG+TGG | - | Chr7:45618144-45618163 | MsG0780038387.01.T01:CDS | 35.0% | |
| AGCCTTTCTTGTTGAATCAT+TGG | + | Chr7:45618085-45618104 | None:intergenic | 35.0% | |
| CTCCAATGATTCAACAAGAA+AGG | - | Chr7:45618080-45618099 | MsG0780038387.01.T01:CDS | 35.0% | |
| GAAAGATGGCAGAAAAAATG+TGG | - | Chr7:45618275-45618294 | MsG0780038387.01.T01:CDS | 35.0% | |
| GCAACAGATTACTAAAGCTA+GGG | - | Chr7:45618317-45618336 | MsG0780038387.01.T01:CDS | 35.0% | |
| GTTGCAAAAGGAAACTTTCT+TGG | + | Chr7:45618302-45618321 | None:intergenic | 35.0% | |
| TCAGAAAGAGTGATATCATC+TGG | + | Chr7:45618426-45618445 | None:intergenic | 35.0% | |
| TGCAACAGATTACTAAAGCT+AGG | - | Chr7:45618316-45618335 | MsG0780038387.01.T01:CDS | 35.0% | |
| ! | ACTTTTCTTCCTCTTCTTGT+AGG | + | Chr7:45618109-45618128 | None:intergenic | 35.0% |
| ! | GAAGGAAATTGATGACAAGA+TGG | - | Chr7:45618482-45618501 | MsG0780038387.01.T01:CDS | 35.0% |
| !! | TTGATTCTAAAACAGAGGTG+TGG | - | Chr7:45618199-45618218 | MsG0780038387.01.T01:CDS | 35.0% |
| AAATCTGGAGGAAGCAAAAC+AGG | - | Chr7:45618224-45618243 | MsG0780038387.01.T01:CDS | 40.0% | |
| AAGCAAAACAGGTGATTGAG+AGG | - | Chr7:45618235-45618254 | MsG0780038387.01.T01:CDS | 40.0% | |
| AGGATCGGAAATTATAGCAC+AGG | + | Chr7:45618178-45618197 | None:intergenic | 40.0% | |
| CTTGTCTTTGCTTTCTCAGT+TGG | + | Chr7:45618344-45618363 | None:intergenic | 40.0% | |
| ! | TGTTTTGCTTCCTCCAGATT+TGG | + | Chr7:45618225-45618244 | None:intergenic | 40.0% |
| AGCTGAACATTCGTATGAGC+TGG | - | Chr7:45618379-45618398 | MsG0780038387.01.T01:CDS | 45.0% | |
| CTGGTACCTGCAAAACAACA+CGG | - | Chr7:45618398-45618417 | MsG0780038387.01.T01:CDS | 45.0% | |
| GAAAGGCTTCCTACAAGAAG+AGG | - | Chr7:45618097-45618116 | MsG0780038387.01.T01:CDS | 45.0% | |
| TCGGAAATTATAGCACAGGC+TGG | + | Chr7:45618174-45618193 | None:intergenic | 45.0% | |
| ! | AACAGAGGTGTGGCCAAATC+TGG | - | Chr7:45618209-45618228 | MsG0780038387.01.T01:CDS | 50.0% |
| ! | AGAGGTGTGGCCAAATCTGG+AGG | - | Chr7:45618212-45618231 | MsG0780038387.01.T01:CDS | 55.0% |
| !!! | CTGGCACCGTGTTGTTTTGC+AGG | + | Chr7:45618407-45618426 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 45618048 | 45618527 | 45618048 | ID=MsG0780038387.01;Name=MsG0780038387.01 |
| Chr7 | mRNA | 45618048 | 45618527 | 45618048 | ID=MsG0780038387.01.T01;Parent=MsG0780038387.01;Name=MsG0780038387.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|159 |
| Chr7 | exon | 45618048 | 45618527 | 45618048 | ID=MsG0780038387.01.T01:exon:11136;Parent=MsG0780038387.01.T01 |
| Chr7 | CDS | 45618048 | 45618527 | 45618048 | ID=MsG0780038387.01.T01:cds;Parent=MsG0780038387.01.T01 |
| Gene Sequence |
| Protein sequence |
