Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039396.01.T01 | XP_039684190.1 | 95.28 | 339 | 13 | 1 | 1 | 336 | 1 | 339 | 0 | 652 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039396.01.T01 | O04351 | 33.243 | 367 | 161 | 11 | 1 | 333 | 1 | 317 | 1.56E-39 | 145 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039396.01.T01 | A0A072U1F0 | 92.550 | 349 | 13 | 2 | 1 | 336 | 1 | 349 | 0.0 | 642 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0780039396.01.T01 | TF | OFP |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039396.01.T01 | MTR_7g072630 | 92.550 | 349 | 13 | 2 | 1 | 336 | 1 | 349 | 0.0 | 642 |
| MsG0780039396.01.T01 | MTR_7g072875 | 91.606 | 274 | 10 | 2 | 76 | 336 | 14 | 287 | 4.34e-177 | 492 |
| MsG0780039396.01.T01 | MTR_4g028930 | 47.912 | 407 | 112 | 16 | 1 | 334 | 1 | 380 | 1.75e-89 | 273 |
| MsG0780039396.01.T01 | MTR_5g075210 | 40.220 | 363 | 169 | 11 | 1 | 333 | 1 | 345 | 4.82e-63 | 204 |
| MsG0780039396.01.T01 | MTR_2g018030 | 60.606 | 66 | 26 | 0 | 268 | 333 | 277 | 342 | 2.79e-25 | 104 |
| MsG0780039396.01.T01 | MTR_8g087530 | 57.576 | 66 | 28 | 0 | 268 | 333 | 258 | 323 | 3.43e-22 | 95.9 |
| MsG0780039396.01.T01 | MTR_8g103520 | 53.226 | 62 | 29 | 0 | 268 | 329 | 224 | 285 | 2.30e-16 | 78.6 |
| MsG0780039396.01.T01 | MTR_0907s0020 | 45.205 | 73 | 40 | 0 | 257 | 329 | 91 | 163 | 1.00e-14 | 72.0 |
| MsG0780039396.01.T01 | MTR_4g052040 | 46.875 | 64 | 34 | 0 | 266 | 329 | 97 | 160 | 1.92e-13 | 68.2 |
| MsG0780039396.01.T01 | MTR_4g132740 | 47.619 | 63 | 33 | 0 | 270 | 332 | 83 | 145 | 4.23e-13 | 66.6 |
| MsG0780039396.01.T01 | MTR_3g111780 | 30.688 | 189 | 101 | 3 | 152 | 329 | 90 | 259 | 1.38e-12 | 67.4 |
| MsG0780039396.01.T01 | MTR_7g105790 | 44.068 | 59 | 33 | 0 | 271 | 329 | 113 | 171 | 3.20e-12 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039396.01.T01 | AT1G06920 | 33.516 | 364 | 157 | 14 | 1 | 334 | 1 | 309 | 6.43e-41 | 145 |
| MsG0780039396.01.T01 | AT2G30400 | 33.243 | 367 | 161 | 11 | 1 | 333 | 1 | 317 | 1.59e-40 | 145 |
| MsG0780039396.01.T01 | AT5G58360 | 31.054 | 351 | 158 | 10 | 1 | 333 | 5 | 289 | 1.82e-35 | 130 |
| MsG0780039396.01.T01 | AT5G01840 | 34.421 | 337 | 141 | 10 | 1 | 332 | 1 | 262 | 7.19e-35 | 128 |
| MsG0780039396.01.T01 | AT4G18830 | 55.385 | 65 | 29 | 0 | 268 | 332 | 282 | 346 | 2.69e-19 | 87.8 |
| MsG0780039396.01.T01 | AT5G19650 | 50.000 | 66 | 33 | 0 | 267 | 332 | 153 | 218 | 9.23e-16 | 75.9 |
| MsG0780039396.01.T01 | AT3G52525 | 46.032 | 63 | 34 | 0 | 269 | 331 | 67 | 129 | 3.25e-13 | 67.0 |
| MsG0780039396.01.T01 | AT2G18500 | 41.791 | 67 | 39 | 0 | 266 | 332 | 224 | 290 | 4.50e-13 | 69.3 |
| MsG0780039396.01.T01 | AT2G36026 | 48.214 | 56 | 29 | 0 | 274 | 329 | 92 | 147 | 6.03e-12 | 63.9 |
Find 40 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACACGGTCTGTTCTGATTTC+TGG | 0.132362 | 7:-63404929 | None:intergenic |
| TCAAAGTGTTCAAGCAAATA+TGG | 0.233458 | 7:+63405530 | MsG0780039396.01.T01:CDS |
| AATGATGAACACAACGTTAA+AGG | 0.235673 | 7:+63405154 | MsG0780039396.01.T01:CDS |
| TTCTTCCTTTGTTGTGTTGT+TGG | 0.243928 | 7:-63404656 | None:intergenic |
| AGAATTGTTCTACTGATTAT+CGG | 0.312127 | 7:-63405563 | None:intergenic |
| CATTAGAACATCAAAGGATT+TGG | 0.325611 | 7:+63405450 | MsG0780039396.01.T01:CDS |
| TGTAGATTCTATTGCTTGTT+TGG | 0.346884 | 7:-63404778 | None:intergenic |
| CGAAAGACTTCTCCGGTTAC+CGG | 0.357509 | 7:-63405344 | None:intergenic |
| TTTGTTGTGTTGTTGGTGTT+TGG | 0.367246 | 7:-63404649 | None:intergenic |
| TGTCGTCTGGTTCCGGTAAC+CGG | 0.384473 | 7:+63405332 | MsG0780039396.01.T01:CDS |
| GAGAGGTAATGGGAAATTAC+AGG | 0.389366 | 7:+63404558 | None:intergenic |
| ATGATGAACACAACGTTAAA+GGG | 0.389606 | 7:+63405155 | MsG0780039396.01.T01:CDS |
| AAAAGTGTGTCGTCTGGTTC+CGG | 0.412570 | 7:+63405325 | MsG0780039396.01.T01:CDS |
| TCTGGTTTGGTTACAATTGG+TGG | 0.419282 | 7:-63405064 | None:intergenic |
| CGGTAACCGGAGAAGTCTTT+CGG | 0.428330 | 7:+63405345 | MsG0780039396.01.T01:CDS |
| GTTCACCAGGTGTGAAACTT+AGG | 0.443025 | 7:+63405254 | MsG0780039396.01.T01:CDS |
| TCTGTTCTGATTTCTGGCTC+AGG | 0.447190 | 7:-63404923 | None:intergenic |
| GGAGATGATGATGAAGTCTT+TGG | 0.474574 | 7:-63404857 | None:intergenic |
| CAGACATGATGCCTAATGCT+TGG | 0.484513 | 7:+63404591 | MsG0780039396.01.T01:CDS |
| CTGTTCTGATTTCTGGCTCA+GGG | 0.485970 | 7:-63404922 | None:intergenic |
| AGCTTGTAAAACCAAGCATT+AGG | 0.493200 | 7:-63404602 | None:intergenic |
| TCGATAATGATATCGTTTGT+CGG | 0.505634 | 7:-63404959 | None:intergenic |
| CTAACAGAAAATCTTCTACT+TGG | 0.507243 | 7:-63405232 | None:intergenic |
| GGCAGGAAAAGTGTGTCGTC+TGG | 0.513012 | 7:+63405319 | MsG0780039396.01.T01:CDS |
| AGGAGATTTCAGAGAATCAA+TGG | 0.515867 | 7:+63405405 | MsG0780039396.01.T01:CDS |
| CTATCAAGTGGAGAATCAAA+TGG | 0.529685 | 7:-63404878 | None:intergenic |
| ACTCATCTGAATTCAAAGAA+AGG | 0.530280 | 7:-63405492 | None:intergenic |
| GGAAAATTCAAACACATGGC+AGG | 0.547159 | 7:+63405302 | MsG0780039396.01.T01:CDS |
| GAACAACATTAGAACATCAA+AGG | 0.551741 | 7:+63405444 | MsG0780039396.01.T01:CDS |
| AACTATCCGAAAGACTTCTC+CGG | 0.559644 | 7:-63405351 | None:intergenic |
| TTTCTTGGATTGCTTTGGTG+GGG | 0.559822 | 7:-63404728 | None:intergenic |
| AAACACCAACAACACAACAA+AGG | 0.563217 | 7:+63404651 | MsG0780039396.01.T01:CDS |
| ATTCACCAAGAATTTCAAGC+AGG | 0.584426 | 7:+63405281 | MsG0780039396.01.T01:CDS |
| GATTCTCTGAAATCTCCTTG+TGG | 0.590560 | 7:-63405400 | None:intergenic |
| GATTCTGCAGAGCTATCAAG+TGG | 0.613063 | 7:-63404890 | None:intergenic |
| AGCAGGAAAATTCAAACACA+TGG | 0.619102 | 7:+63405298 | MsG0780039396.01.T01:CDS |
| TTGATCCTAAGTTTCACACC+TGG | 0.631177 | 7:-63405259 | None:intergenic |
| AGATTTCAGAGAATCAATGG+TGG | 0.652480 | 7:+63405408 | MsG0780039396.01.T01:CDS |
| AAGTCATCATTGAATCCACA+AGG | 0.674680 | 7:+63405385 | MsG0780039396.01.T01:CDS |
| CGTTTGTCGGAAGAAGAACA+CGG | 0.768531 | 7:-63404946 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACTAAAAGTCAAGATTTTGA+AGG | + | Chr7:63405180-63405199 | MsG0780039396.01.T01:CDS | 25.0% |
| !! | ATTTGATGAATTTTTGCTTC+TGG | - | Chr7:63405137-63405156 | None:intergenic | 25.0% |
| !! | GTAAAGTAGTAAGATTTTCT+TGG | - | Chr7:63404746-63404765 | None:intergenic | 25.0% |
| !! | TTTGATGAATTTTTGCTTCT+GGG | - | Chr7:63405136-63405155 | None:intergenic | 25.0% |
| !!! | CTTTGTTTTTGATGTATTTG+AGG | - | Chr7:63404705-63404724 | None:intergenic | 25.0% |
| !!! | TGTTTTGATGATGATTTTCT+TGG | - | Chr7:63404815-63404834 | None:intergenic | 25.0% |
| AATGATGAACACAACGTTAA+AGG | + | Chr7:63405154-63405173 | MsG0780039396.01.T01:CDS | 30.0% | |
| ACTCATCTGAATTCAAAGAA+AGG | - | Chr7:63405495-63405514 | None:intergenic | 30.0% | |
| ATGATGAACACAACGTTAAA+GGG | + | Chr7:63405155-63405174 | MsG0780039396.01.T01:CDS | 30.0% | |
| CATTAGAACATCAAAGGATT+TGG | + | Chr7:63405450-63405469 | MsG0780039396.01.T01:CDS | 30.0% | |
| CTAACAGAAAATCTTCTACT+TGG | - | Chr7:63405235-63405254 | None:intergenic | 30.0% | |
| GAACAACATTAGAACATCAA+AGG | + | Chr7:63405444-63405463 | MsG0780039396.01.T01:CDS | 30.0% | |
| TCGATAATGATATCGTTTGT+CGG | - | Chr7:63404962-63404981 | None:intergenic | 30.0% | |
| TGTAGATTCTATTGCTTGTT+TGG | - | Chr7:63404781-63404800 | None:intergenic | 30.0% | |
| ! | AACAAGTAGTTGAACTTTTC+TGG | - | Chr7:63405085-63405104 | None:intergenic | 30.0% |
| ! | AAGATTTTCTTGGATTGCTT+TGG | - | Chr7:63404736-63404755 | None:intergenic | 30.0% |
| ! | ATTTTCCTGCTTGAAATTCT+TGG | - | Chr7:63405289-63405308 | None:intergenic | 30.0% |
| ! | TCAAAGTGTTCAAGCAAATA+TGG | + | Chr7:63405530-63405549 | MsG0780039396.01.T01:CDS | 30.0% |
| ! | TTTTACAAGCTTAGAGACAT+GGG | + | Chr7:63404614-63404633 | MsG0780039396.01.T01:CDS | 30.0% |
| !!! | TTTGATGATGATTTTCTTGG+TGG | - | Chr7:63404812-63404831 | None:intergenic | 30.0% |
| !!! | TTTTCTGGTTTGGTTACAAT+TGG | - | Chr7:63405070-63405089 | None:intergenic | 30.0% |
| AAACACCAACAACACAACAA+AGG | + | Chr7:63404651-63404670 | MsG0780039396.01.T01:CDS | 35.0% | |
| AAGTCATCATTGAATCCACA+AGG | + | Chr7:63405385-63405404 | MsG0780039396.01.T01:CDS | 35.0% | |
| AGATTTCAGAGAATCAATGG+TGG | + | Chr7:63405408-63405427 | MsG0780039396.01.T01:CDS | 35.0% | |
| AGCAGGAAAATTCAAACACA+TGG | + | Chr7:63405298-63405317 | MsG0780039396.01.T01:CDS | 35.0% | |
| AGCTTGTAAAACCAAGCATT+AGG | - | Chr7:63404605-63404624 | None:intergenic | 35.0% | |
| AGGAGATTTCAGAGAATCAA+TGG | + | Chr7:63405405-63405424 | MsG0780039396.01.T01:CDS | 35.0% | |
| ATTCACCAAGAATTTCAAGC+AGG | + | Chr7:63405281-63405300 | MsG0780039396.01.T01:CDS | 35.0% | |
| CTATCAAGTGGAGAATCAAA+TGG | - | Chr7:63404881-63404900 | None:intergenic | 35.0% | |
| ! | AGATTTTCTGTTAGTTCACC+AGG | + | Chr7:63405241-63405260 | MsG0780039396.01.T01:CDS | 35.0% |
| ! | ATTTTCTTGGATTGCTTTGG+TGG | - | Chr7:63404733-63404752 | None:intergenic | 35.0% |
| ! | GTTTTACAAGCTTAGAGACA+TGG | + | Chr7:63404613-63404632 | MsG0780039396.01.T01:CDS | 35.0% |
| ! | TTCTTCCTTTGTTGTGTTGT+TGG | - | Chr7:63404659-63404678 | None:intergenic | 35.0% |
| ! | TTTGTTGTGTTGTTGGTGTT+TGG | - | Chr7:63404652-63404671 | None:intergenic | 35.0% |
| ! | TTTTCTTGGATTGCTTTGGT+GGG | - | Chr7:63404732-63404751 | None:intergenic | 35.0% |
| !! | AGATTTTGAAGGAAGAACAG+AGG | + | Chr7:63405191-63405210 | MsG0780039396.01.T01:CDS | 35.0% |
| !!! | GTAGTTGAACTTTTCTGGTT+TGG | - | Chr7:63405080-63405099 | None:intergenic | 35.0% |
| !!! | GTCTTTGGTGTTCTTCTTTT+TGG | - | Chr7:63404845-63404864 | None:intergenic | 35.0% |
| AACTATCCGAAAGACTTCTC+CGG | - | Chr7:63405354-63405373 | None:intergenic | 40.0% | |
| GGAAAATTCAAACACATGGC+AGG | + | Chr7:63405302-63405321 | MsG0780039396.01.T01:CDS | 40.0% | |
| TTGATCCTAAGTTTCACACC+TGG | - | Chr7:63405262-63405281 | None:intergenic | 40.0% | |
| TTTCTTGGATTGCTTTGGTG+GGG | - | Chr7:63404731-63404750 | None:intergenic | 40.0% | |
| ! | GGAGATGATGATGAAGTCTT+TGG | - | Chr7:63404860-63404879 | None:intergenic | 40.0% |
| !! | GATTCTCTGAAATCTCCTTG+TGG | - | Chr7:63405403-63405422 | None:intergenic | 40.0% |
| !! | TCTGGTTTGGTTACAATTGG+TGG | - | Chr7:63405067-63405086 | None:intergenic | 40.0% |
| ACACGGTCTGTTCTGATTTC+TGG | - | Chr7:63404932-63404951 | None:intergenic | 45.0% | |
| CAGACATGATGCCTAATGCT+TGG | + | Chr7:63404591-63404610 | MsG0780039396.01.T01:CDS | 45.0% | |
| CGTTTGTCGGAAGAAGAACA+CGG | - | Chr7:63404949-63404968 | None:intergenic | 45.0% | |
| GATTCTGCAGAGCTATCAAG+TGG | - | Chr7:63404893-63404912 | None:intergenic | 45.0% | |
| GTTCACCAGGTGTGAAACTT+AGG | + | Chr7:63405254-63405273 | MsG0780039396.01.T01:CDS | 45.0% | |
| ! | AAAAGTGTGTCGTCTGGTTC+CGG | + | Chr7:63405325-63405344 | MsG0780039396.01.T01:CDS | 45.0% |
| !! | CTGTTCTGATTTCTGGCTCA+GGG | - | Chr7:63404925-63404944 | None:intergenic | 45.0% |
| !! | TCTGTTCTGATTTCTGGCTC+AGG | - | Chr7:63404926-63404945 | None:intergenic | 45.0% |
| CGAAAGACTTCTCCGGTTAC+CGG | - | Chr7:63405347-63405366 | None:intergenic | 50.0% | |
| CGGTAACCGGAGAAGTCTTT+CGG | + | Chr7:63405345-63405364 | MsG0780039396.01.T01:CDS | 50.0% | |
| ! | GGCAGGAAAAGTGTGTCGTC+TGG | + | Chr7:63405319-63405338 | MsG0780039396.01.T01:CDS | 55.0% |
| ! | TGTCGTCTGGTTCCGGTAAC+CGG | + | Chr7:63405332-63405351 | MsG0780039396.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 63404566 | 63405576 | 63404566 | ID=MsG0780039396.01;Name=MsG0780039396.01 |
| Chr7 | mRNA | 63404566 | 63405576 | 63404566 | ID=MsG0780039396.01.T01;Parent=MsG0780039396.01;Name=MsG0780039396.01.T01;_AED=0.21;_eAED=0.21;_QI=0|-1|0|1|-1|1|1|0|336 |
| Chr7 | exon | 63404566 | 63405576 | 63404566 | ID=MsG0780039396.01.T01:exon:15969;Parent=MsG0780039396.01.T01 |
| Chr7 | CDS | 63404566 | 63405576 | 63404566 | ID=MsG0780039396.01.T01:cds;Parent=MsG0780039396.01.T01 |
| Gene Sequence |
| Protein sequence |
