AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780039543.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039543.01.T01 MTR_7g073710 95.238 147 7 0 1 147 280 426 1.74e-96 293
MsG0780039543.01.T01 MTR_7g073710 98.667 75 1 0 143 217 537 611 3.00e-45 159
MsG0780039543.01.T01 MTR_5g075630 69.536 151 38 1 1 143 293 443 5.80e-64 209
MsG0780039543.01.T01 MTR_5g075630 77.465 71 16 0 145 215 560 630 6.11e-33 125
MsG0780039543.01.T01 MTR_3g062500 75.000 120 30 0 25 144 324 443 2.89e-56 189
MsG0780039543.01.T01 MTR_3g062500 79.452 73 15 0 143 215 557 629 4.62e-35 131
MsG0780039543.01.T01 MTR_3g062500 75.000 120 30 0 25 144 334 453 3.41e-56 189
MsG0780039543.01.T01 MTR_3g062500 79.452 73 15 0 143 215 567 639 4.30e-35 131
MsG0780039543.01.T01 MTR_3g093710 59.494 158 57 2 1 151 281 438 6.26e-54 182
MsG0780039543.01.T01 MTR_3g093710 69.014 71 22 0 143 213 545 615 1.62e-28 112
MsG0780039543.01.T01 MTR_5g068210 60.811 148 54 1 1 144 254 401 3.87e-53 180
MsG0780039543.01.T01 MTR_5g068210 62.319 69 25 1 146 213 520 588 4.30e-22 94.4
MsG0780039543.01.T01 MTR_5g009660 63.571 140 50 1 4 143 249 387 3.79e-49 169
MsG0780039543.01.T01 MTR_5g009660 66.667 69 23 0 145 213 505 573 1.30e-26 107
MsG0780039543.01.T01 MTR_4g113100 70.690 116 33 1 28 143 320 434 4.07e-49 170
MsG0780039543.01.T01 MTR_4g113100 75.385 65 16 0 148 212 553 617 3.33e-30 117
MsG0780039543.01.T01 MTR_1g028890 54.967 151 67 1 1 151 237 386 9.35e-47 162
MsG0780039543.01.T01 MTR_1g028890 65.753 73 25 0 141 213 485 557 4.97e-27 108
MsG0780039543.01.T01 MTR_8g099195 60.403 149 44 4 3 143 269 410 6.35e-46 161
MsG0780039543.01.T01 MTR_8g099195 69.014 71 22 0 143 213 526 596 4.62e-29 114
MsG0780039543.01.T01 MTR_3g090665 53.846 143 65 1 1 143 2 143 7.36e-43 147
MsG0780039543.01.T01 MTR_3g090665 68.116 69 22 0 145 213 260 328 7.22e-27 105
MsG0780039543.01.T01 MTR_3g090660 63.636 110 39 1 35 144 301 409 4.18e-41 147
MsG0780039543.01.T01 MTR_3g090660 71.212 66 19 0 148 213 529 594 5.59e-27 108
MsG0780039543.01.T01 MTR_8g107470 60.748 107 41 1 38 144 358 463 2.16e-37 137
MsG0780039543.01.T01 MTR_8g107470 68.056 72 23 0 144 215 577 648 1.10e-29 115
MsG0780039543.01.T01 MTR_5g055470 57.798 109 43 3 37 144 322 428 9.19e-35 130
MsG0780039543.01.T01 MTR_5g055470 63.077 65 24 0 148 212 543 607 6.46e-23 96.7
MsG0780039543.01.T01 MTR_1g110280 58.559 111 43 3 35 144 338 446 9.83e-35 130
MsG0780039543.01.T01 MTR_1g110280 66.197 71 24 0 144 214 569 639 1.34e-27 110
MsG0780039543.01.T01 MTR_6g470960 56.481 108 45 2 38 144 360 466 6.34e-33 125
MsG0780039543.01.T01 MTR_6g470960 62.500 72 27 0 144 215 580 651 1.34e-26 107
MsG0780039543.01.T01 MTR_7g103440 53.982 113 49 3 33 144 354 464 1.31e-32 124
MsG0780039543.01.T01 MTR_7g103440 67.692 65 21 0 149 213 596 660 1.63e-25 103
MsG0780039543.01.T01 MTR_1g061590 54.128 109 47 3 37 144 345 451 4.95e-32 122
MsG0780039543.01.T01 MTR_1g061590 68.116 69 22 0 145 213 579 647 8.35e-28 110
MsG0780039543.01.T01 MTR_5g078080 55.172 116 50 2 39 153 324 438 8.68e-32 122
MsG0780039543.01.T01 MTR_5g078080 53.623 69 32 0 144 212 542 610 3.82e-21 91.3
MsG0780039543.01.T01 MTR_8g087420 51.282 117 52 3 31 143 312 427 1.01e-27 110
MsG0780039543.01.T01 MTR_8g101260 48.214 112 56 2 34 144 320 430 1.32e-26 107
MsG0780039543.01.T01 MTR_4g074080 52.083 96 44 2 49 143 360 454 2.86e-26 106
MsG0780039543.01.T01 MTR_1g009270 47.863 117 59 2 33 148 320 435 2.96e-26 106
MsG0780039543.01.T01 MTR_1g009270 40.541 74 44 0 140 213 542 615 1.74e-11 63.2
MsG0780039543.01.T01 MTR_6g093050 45.536 112 58 3 38 147 322 432 1.19e-23 98.6
MsG0780039543.01.T01 MTR_3g102180 40.146 137 77 2 11 143 327 462 1.57e-23 98.2
MsG0780039543.01.T01 MTR_1g109580 48.515 101 50 2 49 148 398 497 1.99e-23 98.2
MsG0780039543.01.T01 MTR_6g069030 38.255 149 84 3 1 142 267 414 7.28e-23 96.3
MsG0780039543.01.T01 MTR_4g014070 46.610 118 57 5 38 151 486 601 1.80e-22 95.5
MsG0780039543.01.T01 MTR_4g014070 45.714 70 37 1 148 216 709 778 1.05e-14 72.8
MsG0780039543.01.T01 MTR_8g021350 51.042 96 45 2 49 143 390 484 2.22e-22 95.1
MsG0780039543.01.T01 MTR_4g094885 40.870 115 64 2 32 143 336 449 1.37e-21 92.8
MsG0780039543.01.T01 MTR_5g083480 43.220 118 61 5 38 151 478 593 1.38e-21 92.8
MsG0780039543.01.T01 MTR_5g083480 50.000 70 34 1 148 216 701 770 7.03e-17 79.3
MsG0780039543.01.T01 MTR_5g083480 43.220 118 61 5 38 151 481 596 1.38e-21 92.8
MsG0780039543.01.T01 MTR_5g083480 50.000 70 34 1 148 216 704 773 7.04e-17 79.3
MsG0780039543.01.T01 MTR_2g105900 40.171 117 67 3 32 146 318 433 1.27e-20 90.1
MsG0780039543.01.T01 MTR_1g052425 39.103 156 82 7 18 164 307 458 1.80e-20 89.4
MsG0780039543.01.T01 MTR_2g008740 43.363 113 60 4 35 145 93 203 2.44e-20 88.6
MsG0780039543.01.T01 MTR_2g008740 45.455 66 36 0 148 213 323 388 7.96e-13 67.0
MsG0780039543.01.T01 MTR_4g014350 34.356 163 86 5 1 145 344 503 1.46e-19 87.0
MsG0780039543.01.T01 MTR_2g023150 43.820 89 49 1 61 149 1 88 5.08e-19 83.2
MsG0780039543.01.T01 MTR_2g023150 51.515 66 32 0 148 213 185 250 1.05e-15 73.9
MsG0780039543.01.T01 MTR_7g070200 41.584 101 57 2 46 145 416 515 5.93e-19 85.1
MsG0780039543.01.T01 MTR_2g042710 44.330 97 52 2 48 143 346 441 6.52e-19 85.1
MsG0780039543.01.T01 MTR_4g124990 43.750 96 52 2 49 143 344 438 1.80e-17 80.9
MsG0780039543.01.T01 MTR_3g078250 40.860 93 54 1 51 143 365 456 2.55e-17 80.5
MsG0780039543.01.T01 MTR_3g078250 42.029 69 40 0 145 213 564 632 2.05e-12 65.9
MsG0780039543.01.T01 MTR_1g107460 40.860 93 53 2 49 140 451 542 2.21e-16 77.8
MsG0780039543.01.T01 MTR_4g094958 37.634 93 56 2 49 140 456 547 2.31e-15 74.7
MsG0780039543.01.T01 MTR_4g014130 44.286 70 38 1 148 216 1 70 4.82e-15 68.2
MsG0780039543.01.T01 MTR_2g030380 41.489 94 51 3 52 142 762 854 5.59e-14 70.9
MsG0780039543.01.T01 MTR_2g030380 41.489 94 51 3 52 142 780 872 5.61e-14 70.9
MsG0780039543.01.T01 MTR_3g094710 42.553 94 50 3 41 131 737 829 9.48e-14 70.1
MsG0780039543.01.T01 MTR_7g059225 51.429 70 32 1 146 213 591 660 1.11e-13 69.7
MsG0780039543.01.T01 MTR_7g059225 36.111 108 63 4 38 142 364 468 3.77e-12 65.1
MsG0780039543.01.T01 MTR_7g080810 33.636 110 67 2 38 142 280 388 3.56e-13 68.2
MsG0780039543.01.T01 MTR_8g016330 36.441 118 68 3 42 153 445 561 1.22e-12 66.6
MsG0780039543.01.T01 MTR_6g009370 34.615 104 62 2 51 149 294 396 1.70e-12 66.2
MsG0780039543.01.T01 MTR_1g047670 31.532 111 67 3 49 151 801 910 2.12e-12 66.2
MsG0780039543.01.T01 MTR_8g010180 33.636 110 67 2 38 142 284 392 3.41e-12 65.5
MsG0780039543.01.T01 MTR_3g095100 34.545 110 65 3 39 142 853 961 4.87e-12 65.1
MsG0780039543.01.T01 MTR_2g070020 33.028 109 59 4 43 142 794 897 4.87e-12 65.1
MsG0780039543.01.T01 MTR_3g009400 40.230 87 49 3 58 142 907 992 6.00e-12 64.7
MsG0780039543.01.T01 MTR_1g100787 33.058 121 74 3 39 153 971 1090 1.28e-11 63.9
MsG0780039543.01.T01 MTR_4g028090 30.081 123 79 5 25 142 549 669 1.90e-11 63.2
MsG0780039543.01.T01 MTR_3g460810 36.066 122 65 6 44 155 307 425 1.99e-11 63.2
MsG0780039543.01.T01 MTR_5g033820 36.364 88 55 1 58 144 304 391 2.69e-11 62.8
MsG0780039543.01.T01 MTR_5g095970 33.065 124 76 3 36 153 334 456 4.98e-11 62.0
MsG0780039543.01.T01 MTR_7g109670 40.449 89 49 3 58 143 382 469 5.30e-11 62.0
MsG0780039543.01.T01 MTR_2g080080 32.727 110 70 2 35 141 457 565 5.50e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039543.01.T01 AT5G58300 54.229 201 83 2 3 194 303 503 1.49e-61 203
MsG0780039543.01.T01 AT5G58300 74.648 71 18 0 145 215 562 632 1.45e-32 124
MsG0780039543.01.T01 AT5G58300 54.229 201 83 2 3 194 303 503 1.49e-61 203
MsG0780039543.01.T01 AT5G58300 74.648 71 18 0 145 215 562 632 1.45e-32 124
MsG0780039543.01.T01 AT5G58300 54.229 201 83 2 3 194 303 503 1.49e-61 203
MsG0780039543.01.T01 AT5G58300 74.648 71 18 0 145 215 562 632 1.45e-32 124
MsG0780039543.01.T01 AT3G08680 66.000 150 46 1 2 146 282 431 3.70e-61 202
MsG0780039543.01.T01 AT3G08680 49.231 130 49 4 102 215 489 617 1.78e-31 121
MsG0780039543.01.T01 AT3G08680 66.000 150 46 1 2 146 282 431 3.70e-61 202
MsG0780039543.01.T01 AT3G08680 49.231 130 49 4 102 215 489 617 1.78e-31 121
MsG0780039543.01.T01 AT5G05160 65.833 120 41 0 25 144 316 435 1.14e-48 168
MsG0780039543.01.T01 AT5G05160 73.134 67 18 0 147 213 551 617 1.66e-29 115
MsG0780039543.01.T01 AT2G26730 61.871 139 48 3 9 143 300 437 6.83e-48 166
MsG0780039543.01.T01 AT2G26730 51.351 111 50 2 108 215 513 622 1.24e-30 119
MsG0780039543.01.T01 AT4G23740 63.566 129 45 2 16 144 298 424 2.22e-46 162
MsG0780039543.01.T01 AT4G23740 58.667 75 28 1 145 216 541 615 3.24e-24 100
MsG0780039543.01.T01 AT4G23740 63.566 129 45 2 16 144 298 424 2.22e-46 162
MsG0780039543.01.T01 AT4G23740 58.667 75 28 1 145 216 541 615 3.24e-24 100
MsG0780039543.01.T01 AT5G41680 46.980 149 76 2 1 147 2 149 3.65e-37 132
MsG0780039543.01.T01 AT5G41680 32.911 158 88 4 58 212 185 327 2.82e-18 82.0
MsG0780039543.01.T01 AT5G53320 53.237 139 63 2 11 149 264 400 8.57e-37 135
MsG0780039543.01.T01 AT5G53320 56.757 74 32 0 140 213 497 570 6.21e-23 96.7
MsG0780039543.01.T01 AT5G41680 47.586 145 73 2 1 143 2 145 1.13e-36 132
MsG0780039543.01.T01 AT5G41680 32.911 158 88 4 58 212 211 353 2.84e-18 82.4
MsG0780039543.01.T01 AT3G17840 53.226 124 56 2 31 153 340 462 4.08e-35 131
MsG0780039543.01.T01 AT3G17840 39.286 112 61 3 110 215 531 641 8.95e-16 75.9
MsG0780039543.01.T01 AT1G48480 57.944 107 44 1 38 144 353 458 1.48e-34 129
MsG0780039543.01.T01 AT1G48480 47.887 71 36 1 144 213 573 643 9.71e-17 78.6
MsG0780039543.01.T01 AT1G64210 58.182 110 45 1 35 144 286 394 1.76e-34 129
MsG0780039543.01.T01 AT1G64210 48.529 68 34 1 148 214 516 583 7.18e-16 76.3
MsG0780039543.01.T01 AT2G36570 48.734 158 64 7 2 144 291 446 3.94e-34 128
MsG0780039543.01.T01 AT2G36570 66.154 65 22 0 149 213 582 646 9.30e-26 104
MsG0780039543.01.T01 AT1G68400 58.929 112 42 4 35 144 342 451 9.46e-34 127
MsG0780039543.01.T01 AT1G68400 68.493 73 22 1 143 214 566 638 2.39e-27 109
MsG0780039543.01.T01 AT5G24100 51.163 129 56 2 17 145 308 429 4.79e-32 122
MsG0780039543.01.T01 AT5G24100 47.692 65 34 0 148 212 547 611 4.47e-15 73.9
MsG0780039543.01.T01 AT5G16590 48.739 119 57 2 29 144 313 430 1.13e-28 113
MsG0780039543.01.T01 AT5G16590 39.726 73 43 1 144 215 544 616 1.30e-15 75.5
MsG0780039543.01.T01 AT3G02880 50.943 106 51 1 39 144 328 432 1.79e-28 112
MsG0780039543.01.T01 AT3G02880 42.466 73 41 1 144 215 546 618 1.93e-15 75.1
MsG0780039543.01.T01 AT5G43020 37.363 182 105 4 21 194 340 520 4.17e-28 111
MsG0780039543.01.T01 AT1G60630 53.125 96 43 2 49 143 343 437 5.03e-27 108
MsG0780039543.01.T01 AT1G10850 37.576 165 77 4 4 143 281 444 6.23e-27 108
MsG0780039543.01.T01 AT2G07040 39.130 138 64 3 29 147 293 429 1.98e-24 100
MsG0780039543.01.T01 AT4G31250 40.000 160 81 7 32 177 342 500 2.47e-24 100
MsG0780039543.01.T01 AT3G20190 41.353 133 69 4 35 159 351 482 6.09e-24 99.8
MsG0780039543.01.T01 AT3G20190 41.353 133 69 4 35 159 162 293 8.80e-24 98.6
MsG0780039543.01.T01 AT1G50610 45.872 109 57 2 38 145 357 464 1.34e-23 98.6
MsG0780039543.01.T01 AT1G25320 47.573 103 52 2 49 150 398 499 1.82e-23 98.2
MsG0780039543.01.T01 AT3G50230 51.020 98 44 2 49 143 376 472 2.14e-23 98.2
MsG0780039543.01.T01 AT3G50230 51.020 98 44 2 49 143 388 484 2.15e-23 98.2
MsG0780039543.01.T01 AT5G35390 43.860 114 62 2 35 147 336 448 2.66e-22 94.7
MsG0780039543.01.T01 AT5G67200 47.959 98 47 2 49 143 386 482 2.91e-22 94.7
MsG0780039543.01.T01 AT2G01210 46.875 96 49 2 49 143 397 491 1.83e-21 92.4
MsG0780039543.01.T01 AT3G51740 47.222 108 53 4 38 143 520 625 6.03e-21 90.9
MsG0780039543.01.T01 AT3G51740 50.746 67 32 1 148 213 743 809 3.14e-16 77.4
MsG0780039543.01.T01 AT3G42880 43.564 101 55 2 48 147 350 449 2.77e-20 89.0
MsG0780039543.01.T01 AT5G20690 43.299 97 53 2 48 143 376 471 1.93e-19 86.7
MsG0780039543.01.T01 AT3G57830 38.400 125 61 4 32 143 326 447 3.56e-19 85.9
MsG0780039543.01.T01 AT3G57830 38.400 125 61 4 32 143 326 447 3.56e-19 85.9
MsG0780039543.01.T01 AT1G67510 43.564 101 55 2 46 145 400 499 9.18e-19 84.7
MsG0780039543.01.T01 AT3G57830 39.286 112 54 3 46 144 338 448 2.67e-18 83.2
MsG0780039543.01.T01 AT2G42290 42.202 109 56 4 39 143 330 435 1.27e-17 81.3
MsG0780039543.01.T01 AT2G23300 36.036 111 66 2 31 137 430 539 4.10e-17 79.7
MsG0780039543.01.T01 AT5G67280 36.036 111 69 2 29 138 425 534 1.67e-16 78.2
MsG0780039543.01.T01 AT4G34220 33.077 130 82 3 11 137 427 554 4.40e-16 77.0
MsG0780039543.01.T01 AT1G66830 40.625 96 55 2 49 143 390 484 4.79e-15 73.9
MsG0780039543.01.T01 AT4G20940 41.489 94 51 3 52 142 750 842 6.49e-15 73.6
MsG0780039543.01.T01 AT4G37250 36.522 115 65 4 29 137 420 532 1.61e-14 72.4
MsG0780039543.01.T01 AT4G39400 35.000 120 71 3 29 142 851 969 1.15e-13 70.1
MsG0780039543.01.T01 AT2G15300 36.220 127 71 6 19 137 431 555 1.41e-13 69.7
MsG0780039543.01.T01 AT3G24660 50.704 71 33 1 145 213 583 653 1.43e-13 69.3
MsG0780039543.01.T01 AT3G24660 39.252 107 59 4 39 142 357 460 2.21e-13 68.9
MsG0780039543.01.T01 AT2G02220 29.221 154 98 4 6 153 683 831 1.81e-13 69.3
MsG0780039543.01.T01 AT5G10020 39.048 105 60 3 41 142 705 808 4.48e-13 68.2
MsG0780039543.01.T01 AT5G10020 39.048 105 60 3 41 142 753 856 4.52e-13 68.2
MsG0780039543.01.T01 AT5G01890 33.065 124 76 4 29 147 658 779 1.74e-12 66.2
MsG0780039543.01.T01 AT5G07280 31.818 132 83 3 28 153 884 1014 1.01e-11 64.3
MsG0780039543.01.T01 AT4G02010 39.326 89 50 3 58 143 382 469 1.46e-11 63.5
MsG0780039543.01.T01 AT3G13380 36.782 87 53 2 57 142 859 944 1.46e-11 63.5
MsG0780039543.01.T01 AT1G55610 39.080 87 50 3 58 142 860 945 2.47e-11 63.2
MsG0780039543.01.T01 AT1G55610 39.080 87 50 3 58 142 860 945 2.47e-11 63.2
MsG0780039543.01.T01 AT5G48380 28.947 114 75 2 38 146 280 392 3.73e-11 62.4
MsG0780039543.01.T01 AT1G23540 35.227 88 55 2 57 143 372 458 6.14e-11 61.6
MsG0780039543.01.T01 AT2G01950 34.091 88 56 2 57 143 839 925 6.94e-11 61.6
MsG0780039543.01.T01 AT5G07620 33.333 126 77 5 34 155 55 177 7.95e-11 61.2

Find 51 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAACAGATTGCACCCATTT+TGG 0.228137 7:+65584855 None:intergenic
GAGAAGCTTATGGAGGAATT+TGG 0.235509 7:-65585749 MsG0780039543.01.T01:CDS
TGTTGGCTGGTATTAATAAA+TGG 0.327284 7:+65584662 None:intergenic
AACAACTTACAAAGCCATTC+TGG 0.329221 7:-65585606 MsG0780039543.01.T01:CDS
ATGTGGTTGATCTTCCAAAA+TGG 0.335117 7:-65584869 MsG0780039543.01.T01:CDS
TACAAGAAAATGAGAGAAAC+AGG 0.373422 7:-65585718 MsG0780039543.01.T01:CDS
GCACGAGGTGGAACCACATT+TGG 0.373880 7:+65585482 None:intergenic
TTAGGATGATTGAAGACATA+AGG 0.385548 7:-65584689 MsG0780039543.01.T01:CDS
TCCATTGAAGGTCTTGTGTC+AGG 0.391017 7:+65584720 None:intergenic
CTTCAATGGAGGAAGTTGTT+AGG 0.395222 7:-65584707 MsG0780039543.01.T01:CDS
AAAAGAATTTGAACAGCAAA+TGG 0.415144 7:-65585531 MsG0780039543.01.T01:CDS
TTCAAACATTGAAGAAGAAC+TGG 0.423053 7:-65584784 MsG0780039543.01.T01:CDS
AGAAAAGGGAGAGAAGCTTA+TGG 0.424057 7:-65585759 MsG0780039543.01.T01:CDS
AAATGTAGCGCTTAAAGAAA+AGG 0.432318 7:-65585774 MsG0780039543.01.T01:CDS
TAACCTCTTCACAACCACTA+TGG 0.439560 7:+65585574 None:intergenic
CTAACAACTTCCTCCATTGA+AGG 0.448552 7:+65584708 None:intergenic
GGTTAAAAGAAGTGACAGTA+GGG 0.453457 7:-65585556 MsG0780039543.01.T01:CDS
GTTTATGACTACTTCACATC+TGG 0.462542 7:-65585428 MsG0780039543.01.T01:CDS
GAAATTACCATGCAATAACT+TGG 0.481503 7:+65585397 None:intergenic
GCCTGACACAAGACCTTCAA+TGG 0.482452 7:-65584721 MsG0780039543.01.T01:CDS
AGGTTAAAAGAAGTGACAGT+AGG 0.484123 7:-65585557 MsG0780039543.01.T01:CDS
CTCAGCTGAAGTGCTTGGTA+AGG 0.492243 7:-65585639 MsG0780039543.01.T01:CDS
TAGGATGATTGAAGACATAA+GGG 0.493574 7:-65584688 MsG0780039543.01.T01:CDS
AATGTAGCGCTTAAAGAAAA+GGG 0.495899 7:-65585773 MsG0780039543.01.T01:CDS
ACTGGTGCAGATGTTGCAAT+TGG 0.544067 7:-65584766 MsG0780039543.01.T01:CDS
TATGGTCCCCTCTTCCAGAA+TGG 0.545928 7:+65585592 None:intergenic
TTACAAAGCCATTCTGGAAG+AGG 0.546686 7:-65585600 MsG0780039543.01.T01:CDS
CTTATGGAGGAATTTGGAAG+TGG 0.554132 7:-65585743 MsG0780039543.01.T01:CDS
AGCAAATGGAAATTGTTCAA+AGG 0.555150 7:-65585517 MsG0780039543.01.T01:CDS
TACAAAGCCATTCTGGAAGA+GGG 0.555560 7:-65585599 MsG0780039543.01.T01:CDS
AGAGCCTCAGCTGAAGTGCT+TGG 0.562044 7:-65585644 MsG0780039543.01.T01:CDS
TGTGGTTGATCTTCCAAAAT+GGG 0.564614 7:-65584868 MsG0780039543.01.T01:CDS
AAAGGGAGAGAAGCTTATGG+AGG 0.579263 7:-65585756 MsG0780039543.01.T01:CDS
TCAGCTGAAGTGCTTGGTAA+GGG 0.579614 7:-65585638 MsG0780039543.01.T01:CDS
TCGTGCTTACTATTATTCCA+AGG 0.591176 7:-65585465 MsG0780039543.01.T01:CDS
CTTACCAAGCACTTCAGCTG+AGG 0.597831 7:+65585640 None:intergenic
GTGCTTGGTAAGGGAAGTTG+TGG 0.598442 7:-65585629 MsG0780039543.01.T01:CDS
GGTGCAATCTGTTGTTAGAG+AGG 0.602134 7:-65584847 MsG0780039543.01.T01:CDS
TCTGGAAGAGGGGACCATAG+TGG 0.608866 7:-65585588 MsG0780039543.01.T01:CDS
GGACCATAGTGGTTGTGAAG+AGG 0.623621 7:-65585577 MsG0780039543.01.T01:CDS
GCAGATGTTGCAATTGGCAA+TGG 0.624057 7:-65584760 MsG0780039543.01.T01:CDS
GAGGTGGAACCACATTTGGA+TGG 0.625860 7:+65585486 None:intergenic
GCTTGATCACCATCCAAATG+TGG 0.644011 7:-65585495 MsG0780039543.01.T01:CDS
GAATAATAGTAAGCACGAGG+TGG 0.652079 7:+65585470 None:intergenic
ACAAAGCCATTCTGGAAGAG+GGG 0.655386 7:-65585598 MsG0780039543.01.T01:CDS
GTAATCAGGGCATGATGATG+TGG 0.661426 7:-65584886 MsG0780039543.01.T01:intron
AATCTGTTGTTAGAGAGGAA+TGG 0.682504 7:-65584842 MsG0780039543.01.T01:CDS
TAGAGAGGAATGGACTGCCG+AGG 0.685363 7:-65584832 MsG0780039543.01.T01:CDS
TGACACAAGACCTTCAATGG+AGG 0.693009 7:-65584718 MsG0780039543.01.T01:CDS
TAGATCAAGATCAAACACCT+CGG 0.707557 7:+65584815 None:intergenic
TTGGAATAATAGTAAGCACG+AGG 0.733664 7:+65585467 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAAGAATTTGAACAGCAAA+TGG - Chr7:65584952-65584971 MsG0780039543.01.T01:intron 25.0%
!! AAGTCTTATATGTAATTTGC+TGG - Chr7:65585187-65585206 MsG0780039543.01.T01:intron 25.0%
AAATGTAGCGCTTAAAGAAA+AGG - Chr7:65584709-65584728 MsG0780039543.01.T01:CDS 30.0%
AATGTAGCGCTTAAAGAAAA+GGG - Chr7:65584710-65584729 MsG0780039543.01.T01:CDS 30.0%
AGCAAATGGAAATTGTTCAA+AGG - Chr7:65584966-65584985 MsG0780039543.01.T01:intron 30.0%
ATCATATGAAACATGATGAC+AGG - Chr7:65585230-65585249 MsG0780039543.01.T01:intron 30.0%
ATTGGATATGATCTTCAATC+TGG + Chr7:65585294-65585313 None:intergenic 30.0%
GAAATTACCATGCAATAACT+TGG + Chr7:65585089-65585108 None:intergenic 30.0%
TACAAGAAAATGAGAGAAAC+AGG - Chr7:65584765-65584784 MsG0780039543.01.T01:CDS 30.0%
TAGGATGATTGAAGACATAA+GGG - Chr7:65585795-65585814 MsG0780039543.01.T01:CDS 30.0%
TTAGGATGATTGAAGACATA+AGG - Chr7:65585794-65585813 MsG0780039543.01.T01:CDS 30.0%
TTCAAACATTGAAGAAGAAC+TGG - Chr7:65585699-65585718 MsG0780039543.01.T01:CDS 30.0%
! AACTCTTTTTGGATTTCACA+AGG - Chr7:65585119-65585138 MsG0780039543.01.T01:intron 30.0%
! CTCTCATTTTCAACTCTTTT+TGG - Chr7:65585108-65585127 MsG0780039543.01.T01:intron 30.0%
!! TGCTGTTTCAAGAAAAAAGT+AGG - Chr7:65584680-65584699 MsG0780039543.01.T01:CDS 30.0%
!!! AACAGGCTAACATTTTTTGA+AGG - Chr7:65584782-65584801 MsG0780039543.01.T01:CDS 30.0%
!!! AACTACTAGTTTTTCATCCT+TGG + Chr7:65585038-65585057 None:intergenic 30.0%
AAAACACCAGTTCAGTAATC+AGG - Chr7:65585583-65585602 MsG0780039543.01.T01:CDS 35.0%
AAACACCAGTTCAGTAATCA+GGG - Chr7:65585584-65585603 MsG0780039543.01.T01:CDS 35.0%
AACAACTTACAAAGCCATTC+TGG - Chr7:65584877-65584896 MsG0780039543.01.T01:CDS 35.0%
AAGTCTGATGTATACAGCTT+TGG - Chr7:65585532-65585551 MsG0780039543.01.T01:CDS 35.0%
AATCTGTTGTTAGAGAGGAA+TGG - Chr7:65585641-65585660 MsG0780039543.01.T01:CDS 35.0%
AGGTTAAAAGAAGTGACAGT+AGG - Chr7:65584926-65584945 MsG0780039543.01.T01:intron 35.0%
ATGTGGTTGATCTTCCAAAA+TGG - Chr7:65585614-65585633 MsG0780039543.01.T01:CDS 35.0%
CTTCTCTCAATAGATCTTCA+AGG - Chr7:65585400-65585419 MsG0780039543.01.T01:intron 35.0%
GAGACACATAAACTTGTAAG+AGG + Chr7:65585451-65585470 None:intergenic 35.0%
GATTTCACAAGGTTCAAGAA+TGG - Chr7:65585130-65585149 MsG0780039543.01.T01:intron 35.0%
GGTTAAAAGAAGTGACAGTA+GGG - Chr7:65584927-65584946 MsG0780039543.01.T01:intron 35.0%
GTTTATGACTACTTCACATC+TGG - Chr7:65585055-65585074 MsG0780039543.01.T01:intron 35.0%
TAGATCAAGATCAAACACCT+CGG + Chr7:65585671-65585690 None:intergenic 35.0%
TCGTGCTTACTATTATTCCA+AGG - Chr7:65585018-65585037 MsG0780039543.01.T01:intron 35.0%
TGGAGGAATTCAACTAGATT+GGG - Chr7:65585265-65585284 MsG0780039543.01.T01:intron 35.0%
TGTGGTTGATCTTCCAAAAT+GGG - Chr7:65585615-65585634 MsG0780039543.01.T01:CDS 35.0%
TTGGAATAATAGTAAGCACG+AGG + Chr7:65585019-65585038 None:intergenic 35.0%
! AGCTTTTCCAAGTTATTGCA+TGG - Chr7:65585079-65585098 MsG0780039543.01.T01:intron 35.0%
! ATCTATTGAGAGAAGCACAT+TGG + Chr7:65585395-65585414 None:intergenic 35.0%
! TGGATGCTTTTATATTGCCA+TGG + Chr7:65585375-65585394 None:intergenic 35.0%
AATGGAAGGAAACATGTCCA+TGG - Chr7:65585355-65585374 MsG0780039543.01.T01:intron 40.0%
AGAAAAGGGAGAGAAGCTTA+TGG - Chr7:65584724-65584743 MsG0780039543.01.T01:CDS 40.0%
ATGACAGGAACAAGTGAAAC+TGG - Chr7:65585245-65585264 MsG0780039543.01.T01:intron 40.0%
ATGGACATGTTTCCTTCCAT+TGG + Chr7:65585356-65585375 None:intergenic 40.0%
CTAACAACTTCCTCCATTGA+AGG + Chr7:65585778-65585797 None:intergenic 40.0%
CTGGAGGAATTCAACTAGAT+TGG - Chr7:65585264-65585283 MsG0780039543.01.T01:intron 40.0%
CTTATGGAGGAATTTGGAAG+TGG - Chr7:65584740-65584759 MsG0780039543.01.T01:CDS 40.0%
CTTCAATGGAGGAAGTTGTT+AGG - Chr7:65585776-65585795 MsG0780039543.01.T01:CDS 40.0%
GAAGATCATATCCAATGTGC+TGG - Chr7:65585298-65585317 MsG0780039543.01.T01:intron 40.0%
GAATAATAGTAAGCACGAGG+TGG + Chr7:65585016-65585035 None:intergenic 40.0%
GAGAAGCTTATGGAGGAATT+TGG - Chr7:65584734-65584753 MsG0780039543.01.T01:CDS 40.0%
TAACCTCTTCACAACCACTA+TGG + Chr7:65584912-65584931 None:intergenic 40.0%
TTCTTCTTGAGATGCTGACA+GGG - Chr7:65585560-65585579 MsG0780039543.01.T01:CDS 40.0%
! ACAACAGATTGCACCCATTT+TGG + Chr7:65585631-65585650 None:intergenic 40.0%
! CAAGGCTGCATATCAGATTT+TGG - Chr7:65585418-65585437 MsG0780039543.01.T01:CDS 40.0%
! TACAAAGCCATTCTGGAAGA+GGG - Chr7:65584884-65584903 MsG0780039543.01.T01:intron 40.0%
! TTACAAAGCCATTCTGGAAG+AGG - Chr7:65584883-65584902 MsG0780039543.01.T01:intron 40.0%
AAAGGGAGAGAAGCTTATGG+AGG - Chr7:65584727-65584746 MsG0780039543.01.T01:CDS 45.0%
ACAGGAACAAGTGAAACTGG+AGG - Chr7:65585248-65585267 MsG0780039543.01.T01:intron 45.0%
ACTGGTGCAGATGTTGCAAT+TGG - Chr7:65585717-65585736 MsG0780039543.01.T01:CDS 45.0%
ATATCCACTCAGCCAATGGA+AGG - Chr7:65585341-65585360 MsG0780039543.01.T01:intron 45.0%
CTTCTTCTTGAGATGCTGAC+AGG - Chr7:65585559-65585578 MsG0780039543.01.T01:CDS 45.0%
GCAGATGTTGCAATTGGCAA+TGG - Chr7:65585723-65585742 MsG0780039543.01.T01:CDS 45.0%
GCTTGATCACCATCCAAATG+TGG - Chr7:65584988-65585007 MsG0780039543.01.T01:intron 45.0%
GGTGCAATCTGTTGTTAGAG+AGG - Chr7:65585636-65585655 MsG0780039543.01.T01:CDS 45.0%
TCATGCCCTGATTACTGAAC+TGG + Chr7:65585592-65585611 None:intergenic 45.0%
TGACACAAGACCTTCAATGG+AGG - Chr7:65585765-65585784 MsG0780039543.01.T01:CDS 45.0%
TTTCTGCTTTCGTTCACCTC+CGG + Chr7:65585502-65585521 None:intergenic 45.0%
! ACAAAGCCATTCTGGAAGAG+GGG - Chr7:65584885-65584904 MsG0780039543.01.T01:intron 45.0%
! GTAATCAGGGCATGATGATG+TGG - Chr7:65585597-65585616 MsG0780039543.01.T01:CDS 45.0%
! TCAGCTGAAGTGCTTGGTAA+GGG - Chr7:65584845-65584864 MsG0780039543.01.T01:CDS 45.0%
! TCCATTGAAGGTCTTGTGTC+AGG + Chr7:65585766-65585785 None:intergenic 45.0%
! TCCATTGGCTGAGTGGATAT+GGG + Chr7:65585341-65585360 None:intergenic 45.0%
! TTCCATTGGCTGAGTGGATA+TGG + Chr7:65585342-65585361 None:intergenic 45.0%
CAATGTGCTGGAGCTGCTAA+GGG - Chr7:65585310-65585329 MsG0780039543.01.T01:intron 50.0%
CTTACCAAGCACTTCAGCTG+AGG + Chr7:65584846-65584865 None:intergenic 50.0%
GAGGTGGAACCACATTTGGA+TGG + Chr7:65585000-65585019 None:intergenic 50.0%
GCCTGACACAAGACCTTCAA+TGG - Chr7:65585762-65585781 MsG0780039543.01.T01:CDS 50.0%
GGACCATAGTGGTTGTGAAG+AGG - Chr7:65584906-65584925 MsG0780039543.01.T01:intron 50.0%
GTGCTTGGTAAGGGAAGTTG+TGG - Chr7:65584854-65584873 MsG0780039543.01.T01:CDS 50.0%
TATGGTCCCCTCTTCCAGAA+TGG + Chr7:65584894-65584913 None:intergenic 50.0%
! CTCAGCTGAAGTGCTTGGTA+AGG - Chr7:65584844-65584863 MsG0780039543.01.T01:CDS 50.0%
! GTTTCCTTCCATTGGCTGAG+TGG + Chr7:65585348-65585367 None:intergenic 50.0%
CCAATGTGCTGGAGCTGCTA+AGG - Chr7:65585309-65585328 MsG0780039543.01.T01:intron 55.0%
CCTTAGCAGCTCCAGCACAT+TGG + Chr7:65585312-65585331 None:intergenic 55.0%
GCACGAGGTGGAACCACATT+TGG + Chr7:65585004-65585023 None:intergenic 55.0%
GCCCATATCCACTCAGCCAA+TGG - Chr7:65585337-65585356 MsG0780039543.01.T01:intron 55.0%
GTCTCACCCAAAAGTCCTCC+AGG - Chr7:65585466-65585485 MsG0780039543.01.T01:CDS 55.0%
TCTGGAAGAGGGGACCATAG+TGG - Chr7:65584895-65584914 MsG0780039543.01.T01:intron 55.0%
TTTCGTTCACCTCCGGTGCT+CGG + Chr7:65585495-65585514 None:intergenic 55.0%
! AGAGCCTCAGCTGAAGTGCT+TGG - Chr7:65584839-65584858 MsG0780039543.01.T01:CDS 55.0%
! CGGTAGCCTGGAGGACTTTT+GGG + Chr7:65585475-65585494 None:intergenic 55.0%
! TCGGTAGCCTGGAGGACTTT+TGG + Chr7:65585476-65585495 None:intergenic 55.0%
!! TAGAGAGGAATGGACTGCCG+AGG - Chr7:65585651-65585670 MsG0780039543.01.T01:CDS 55.0%
TCCTCCAGGCTACCGAGCAC+CGG - Chr7:65585480-65585499 MsG0780039543.01.T01:CDS 65.0%
ACCTCCGGTGCTCGGTAGCC+TGG + Chr7:65585487-65585506 None:intergenic 70.0%
TCCGGTGCTCGGTAGCCTGG+AGG + Chr7:65585484-65585503 None:intergenic 70.0%
!! TCCAGGCTACCGAGCACCGG+AGG - Chr7:65585483-65585502 MsG0780039543.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr7 gene 65584674 65585831 65584674 ID=MsG0780039543.01;Name=MsG0780039543.01
Chr7 mRNA 65584674 65585831 65584674 ID=MsG0780039543.01.T01;Parent=MsG0780039543.01;Name=MsG0780039543.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|0.5|2|0|217
Chr7 exon 65585405 65585831 65585405 ID=MsG0780039543.01.T01:exon:10392;Parent=MsG0780039543.01.T01
Chr7 exon 65584674 65584900 65584674 ID=MsG0780039543.01.T01:exon:10391;Parent=MsG0780039543.01.T01
Chr7 CDS 65585405 65585831 65585405 ID=MsG0780039543.01.T01:cds;Parent=MsG0780039543.01.T01
Chr7 CDS 65584674 65584900 65584674 ID=MsG0780039543.01.T01:cds;Parent=MsG0780039543.01.T01
Gene Sequence

>MsG0780039543.01.T01

ATGTTCTGCTGTTTCAAGAAAAAAGTAGGTGAACAAAATGTAGCGCTTAAAGAAAAGGGAGAGAAGCTTATGGAGGAATTTGGAAGTGGTGTACAAGAAAATGAGAGAAACAGGCTAACATTTTTTGAAGGCTGTGCTTATAATTTTGATCTTGATGATTTGTTAAGAGCCTCAGCTGAAGTGCTTGGTAAGGGAAGTTGTGGAACAACTTACAAAGCCATTCTGGAAGAGGGGACCATAGTGGTTGTGAAGAGGTTAAAAGAAGTGACAGTAGGGAAAAAAGAATTTGAACAGCAAATGGAAATTGTTCAAAGGCTTGATCACCATCCAAATGTGGTTCCACCTCGTGCTTACTATTATTCCAAGGATGAAAAACTAGTAGTTTATGACTACTTCACATCTGGCAGCTTTTCCAAGTTATTGCATGGGCATGATGATGTGGTTGATCTTCCAAAATGGGTGCAATCTGTTGTTAGAGAGGAATGGACTGCCGAGGTGTTTGATCTTGATCTAATGAGATATTCAAACATTGAAGAAGAACTGGTGCAGATGTTGCAATTGGCAATGGCATGTGTAGCAGAGATGCCTGACACAAGACCTTCAATGGAGGAAGTTGTTAGGATGATTGAAGACATAAGGGCATCCATTTATTAA
Protein sequence

>MsG0780039543.01.T01

MFCCFKKKVGEQNVALKEKGEKLMEEFGSGVQENERNRLTFFEGCAYNFDLDDLLRASAEVLGKGSCGTTYKAILEEGTIVVVKRLKEVTVGKKEFEQQMEIVQRLDHHPNVVPPRAYYYSKDEKLVVYDYFTSGSFSKLLHGHDDVVDLPKWVQSVVREEWTAEVFDLDLMRYSNIEEELVQMLQLAMACVAEMPDTRPSMEEVVRMIEDIRASIY*