AlfalfaGEDB | MsG0780039560.01

M. sativa cultivar ZhongmuNo.1 / MsG0780039560.01

KEGG: -
GO: -
PFAM: PF03101

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039560.01.T01	GAU43339.1	50	90	30	1	1	75	60	149	7.58E-19	89.4
MsG0780039560.01.T02	GAU43339.1	60	90	36	0	1	90	60	149	1.89E-28	115

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039560.01.T01	A0A2Z6PEJ1	50.000	90	30	1	1	75	60	149	3.62e-19	89.4
MsG0780039560.01.T02	A0A2Z6PEJ1	60.000	90	36	0	1	90	60	149	9.00e-29	115

TFs/TRs:

Gene ID	Type	Classification
MsG0780039560.01.T02	TF	FAR1

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039560.01.T01	MTR_6g463250	48.000	75	39	0	1	75	73	147	1.36e-21	82.8
MsG0780039560.01.T01	MTR_3g448650	38.462	91	41	1	1	76	67	157	3.37e-16	69.3
MsG0780039560.01.T01	MTR_1g070355	37.500	88	40	1	3	75	61	148	1.15e-14	67.4
MsG0780039560.01.T01	MTR_2g101460	36.667	90	42	1	1	75	30	119	5.74e-13	61.2
MsG0780039560.01.T01	MTR_2g101460	36.667	90	42	1	1	75	40	129	6.89e-13	60.8
MsG0780039560.01.T01	MTR_0060s0090	40.845	71	27	1	19	74	59	129	2.40e-12	58.5
MsG0780039560.01.T01	MTR_1g052260	39.130	69	42	0	1	69	61	129	3.55e-12	58.2
MsG0780039560.01.T01	MTR_8g066350	36.709	79	39	3	1	75	64	135	2.62e-11	56.2
MsG0780039560.01.T02	MTR_1g070355	47.727	88	46	0	3	90	61	148	2.63e-24	95.5
MsG0780039560.01.T02	MTR_3g448650	45.556	90	49	0	1	90	67	156	2.76e-24	90.5
MsG0780039560.01.T02	MTR_0060s0090	53.521	71	33	0	19	89	59	129	1.34e-22	85.9
MsG0780039560.01.T02	MTR_2g101460	44.444	90	50	0	1	90	30	119	4.16e-20	80.5
MsG0780039560.01.T02	MTR_2g101460	44.444	90	50	0	1	90	40	129	5.10e-20	80.5
MsG0780039560.01.T02	MTR_6g463250	42.222	90	37	2	1	90	73	147	5.47e-19	77.0
MsG0780039560.01.T02	MTR_1g052260	39.560	91	55	0	1	91	61	151	8.02e-19	75.9
MsG0780039560.01.T02	MTR_6g053140	45.455	88	41	2	2	89	89	169	7.66e-17	72.0
MsG0780039560.01.T02	MTR_5g076070	48.571	70	36	0	21	90	58	127	1.28e-15	69.7
MsG0780039560.01.T02	MTR_6g022080	36.364	88	56	0	3	90	72	159	1.56e-14	66.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 20 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATATTCACCTTCAATATATC+AGG	0.278248	7:-65854674	None:intergenic
TTTGTTCTAATGAGGGTTAC+AGG	0.288446	7:+65854855	MsG0780039560.01.T02:CDS
CAAAGTAGGTGTGGTTTGTT+TGG	0.365962	7:+65854926	MsG0780039560.01.T02:CDS
TGACGTCTTGGTTCACGCTT+TGG	0.373066	7:-65854905	None:intergenic
AATATTAGCATTGAAGATGT+TGG	0.390341	7:+65854701	MsG0780039560.01.T02:CDS
AACATTTGTTTGTTCTAATG+AGG	0.413090	7:+65854847	MsG0780039560.01.T02:CDS
TCCGCAAAAGCAAGCTTGTT+AGG	0.420307	7:+65854798	MsG0780039560.01.T02:CDS
CTGCATATCAATTCTATAGT+TGG	0.443998	7:+65854750	MsG0780039560.01.T02:CDS
CTTGGTTCACGCTTTGGAGT+TGG	0.445770	7:-65854899	None:intergenic
AGTTGGTATTCAAGATTGAA+TGG	0.446191	7:+65854767	MsG0780039560.01.T02:CDS
CCACACCTACTTTGACGTCT+TGG	0.457690	7:-65854917	None:intergenic
ATATTAGCATTGAAGATGTT+GGG	0.457772	7:+65854702	MsG0780039560.01.T02:CDS
CAAGCTTGTTAGGAATGTAA+AGG	0.494002	7:+65854808	MsG0780039560.01.T02:CDS
AAGCTTGTTAGGAATGTAAA+GGG	0.507228	7:+65854809	MsG0780039560.01.T02:CDS
ACATTTGTTTGTTCTAATGA+GGG	0.540461	7:+65854848	MsG0780039560.01.T02:CDS
TTGTTCTAATGAGGGTTACA+GGG	0.548367	7:+65854856	MsG0780039560.01.T02:CDS
TGGTATGCCTGATATATTGA+AGG	0.592507	7:+65854667	None:intergenic
GTGAACCAAGACGTCAAAGT+AGG	0.607924	7:+65854912	MsG0780039560.01.T02:CDS
CCAAGACGTCAAAGTAGGTG+TGG	0.628380	7:+65854917	MsG0780039560.01.T02:CDS
TTAGCATTGAAGATGTTGGG+AGG	0.679576	7:+65854705	MsG0780039560.01.T02:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AACATTTGTTTGTTCTAATG+AGG	+	Chr7:65854847-65854866	MsG0780039560.01.T02:CDS	25.0%
!	ACATTTGTTTGTTCTAATGA+GGG	+	Chr7:65854848-65854867	MsG0780039560.01.T02:CDS	25.0%
!	ATATTCACCTTCAATATATC+AGG	-	Chr7:65854677-65854696	None:intergenic	25.0%
!!	AATATTAGCATTGAAGATGT+TGG	+	Chr7:65854701-65854720	MsG0780039560.01.T02:CDS	25.0%
!!	ATATTAGCATTGAAGATGTT+GGG	+	Chr7:65854702-65854721	MsG0780039560.01.T02:CDS	25.0%
	CTGCATATCAATTCTATAGT+TGG	+	Chr7:65854750-65854769	MsG0780039560.01.T02:CDS	30.0%
!	AGTTGGTATTCAAGATTGAA+TGG	+	Chr7:65854767-65854786	MsG0780039560.01.T02:CDS	30.0%
!!	AAGCTTGTTAGGAATGTAAA+GGG	+	Chr7:65854809-65854828	MsG0780039560.01.T02:CDS	30.0%
	TTGTTCTAATGAGGGTTACA+GGG	+	Chr7:65854856-65854875	MsG0780039560.01.T02:CDS	35.0%
	TTTGTTCTAATGAGGGTTAC+AGG	+	Chr7:65854855-65854874	MsG0780039560.01.T02:CDS	35.0%
!!	CAAGCTTGTTAGGAATGTAA+AGG	+	Chr7:65854808-65854827	MsG0780039560.01.T02:CDS	35.0%
!	TTAGCATTGAAGATGTTGGG+AGG	+	Chr7:65854705-65854724	MsG0780039560.01.T02:CDS	40.0%
!!	CAAAGTAGGTGTGGTTTGTT+TGG	+	Chr7:65854926-65854945	MsG0780039560.01.T02:CDS	40.0%
!!	TCCTAACAAGCTTGCTTTTG+CGG	-	Chr7:65854802-65854821	None:intergenic	40.0%
	GTGAACCAAGACGTCAAAGT+AGG	+	Chr7:65854912-65854931	MsG0780039560.01.T02:CDS	45.0%
!	TCCGCAAAAGCAAGCTTGTT+AGG	+	Chr7:65854798-65854817	MsG0780039560.01.T02:CDS	45.0%
	CCAAGACGTCAAAGTAGGTG+TGG	+	Chr7:65854917-65854936	MsG0780039560.01.T02:CDS	50.0%
	CCACACCTACTTTGACGTCT+TGG	-	Chr7:65854920-65854939	None:intergenic	50.0%
	TGACGTCTTGGTTCACGCTT+TGG	-	Chr7:65854908-65854927	None:intergenic	50.0%
!!	CTTGGTTCACGCTTTGGAGT+TGG	-	Chr7:65854902-65854921	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	65854671	65854952	65854671	ID=MsG0780039560.01;Name=MsG0780039560.01
Chr7	mRNA	65854671	65854952	65854671	ID=MsG0780039560.01.T01;Parent=MsG0780039560.01;Name=MsG0780039560.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|0\|0\|1\|0\|0\|2\|0\|78
Chr7	exon	65854671	65854823	65854671	ID=MsG0780039560.01.T01:exon:17993;Parent=MsG0780039560.01.T01
Chr7	exon	65854869	65854952	65854869	ID=MsG0780039560.01.T01:exon:17994;Parent=MsG0780039560.01.T01
Chr7	CDS	65854671	65854823	65854671	ID=MsG0780039560.01.T01:cds;Parent=MsG0780039560.01.T01
Chr7	CDS	65854869	65854952	65854869	ID=MsG0780039560.01.T01:cds;Parent=MsG0780039560.01.T01
Chr7	mRNA	65854671	65854952	65854671	ID=MsG0780039560.01.T02;Parent=MsG0780039560.01;Name=MsG0780039560.01.T02;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|93
Chr7	exon	65854671	65854952	65854671	ID=MsG0780039560.01.T02:exon:17995;Parent=MsG0780039560.01.T02
Chr7	CDS	65854671	65854952	65854671	ID=MsG0780039560.01.T02:cds;Parent=MsG0780039560.01.T02

Gene Sequence

>MsG0780039560.01.T01

ATGCCTGATATATTGAAGGTGAATATGAAAAATATTAGCATTGAAGATGTTGGGAGGTATGATTTTTTAAATTTAGAAACTGCATATCAATTCTATAGTTGGTATTCAAGATTGAATGGTTTCTCTGTCCGCAAAAGCAAGCTTGTTAGGAATGGTTACAGGGAAACTAATGAGAGAACTACGCCAACTCCAAAGCGTGAACCAAGACGTCAAAGTAGGTGTGGTTTGTTTGGCTAA

>MsG0780039560.01.T02

ATGCCTGATATATTGAAGGTGAATATGAAAAATATTAGCATTGAAGATGTTGGGAGGTATGATTTTTTAAATTTAGAAACTGCATATCAATTCTATAGTTGGTATTCAAGATTGAATGGTTTCTCTGTCCGCAAAAGCAAGCTTGTTAGGAATGTAAAGGGTGAGAAGTTGCAACAAACATTTGTTTGTTCTAATGAGGGTTACAGGGAAACTAATGAGAGAACTACGCCAACTCCAAAGCGTGAACCAAGACGTCAAAGTAGGTGTGGTTTGTTTGGCTAA

Protein sequence

>MsG0780039560.01.T01

MPDILKVNMKNISIEDVGRYDFLNLETAYQFYSWYSRLNGFSVRKSKLVRNGYRETNERTTPTPKREPRRQSRCGLFG*

>MsG0780039560.01.T02

MPDILKVNMKNISIEDVGRYDFLNLETAYQFYSWYSRLNGFSVRKSKLVRNVKGEKLQQTFVCSNEGYRETNERTTPTPKREPRRQSRCGLFG*