Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039770.01.T01 | XP_003624081.1 | 81.548 | 168 | 7 | 1 | 3 | 146 | 161 | 328 | 1.32E-81 | 253 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039770.01.T01 | O04609 | 46.552 | 174 | 47 | 6 | 2 | 141 | 134 | 295 | 8.09E-34 | 122 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039770.01.T01 | G7L6P2 | 81.548 | 168 | 7 | 1 | 3 | 146 | 161 | 328 | 6.32e-82 | 253 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0780039770.01.T01 | TF | WRKY |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039770.01.T01 | MTR_7g079040 | 81.548 | 168 | 7 | 1 | 3 | 146 | 161 | 328 | 1.60e-85 | 253 |
| MsG0780039770.01.T01 | MTR_8g028565 | 46.512 | 172 | 48 | 4 | 3 | 130 | 162 | 333 | 3.99e-32 | 116 |
| MsG0780039770.01.T01 | MTR_2g032030 | 53.125 | 96 | 35 | 3 | 2 | 87 | 273 | 368 | 1.50e-21 | 89.4 |
| MsG0780039770.01.T01 | MTR_2g032030 | 53.684 | 95 | 34 | 3 | 3 | 87 | 274 | 368 | 1.78e-21 | 89.7 |
| MsG0780039770.01.T01 | MTR_8g087000 | 85.714 | 42 | 6 | 0 | 3 | 44 | 84 | 125 | 2.31e-20 | 84.0 |
| MsG0780039770.01.T01 | MTR_8g087000 | 85.714 | 42 | 6 | 0 | 3 | 44 | 83 | 124 | 2.58e-20 | 84.0 |
| MsG0780039770.01.T01 | MTR_2g016770 | 45.192 | 104 | 45 | 2 | 3 | 100 | 34 | 131 | 3.30e-20 | 82.8 |
| MsG0780039770.01.T01 | MTR_2g016770 | 45.192 | 104 | 45 | 2 | 3 | 100 | 42 | 139 | 3.51e-20 | 82.8 |
| MsG0780039770.01.T01 | MTR_8g098945 | 64.516 | 62 | 18 | 2 | 3 | 63 | 136 | 194 | 6.10e-20 | 83.6 |
| MsG0780039770.01.T01 | MTR_7g109600 | 61.905 | 63 | 22 | 1 | 3 | 65 | 60 | 120 | 7.26e-20 | 83.2 |
| MsG0780039770.01.T01 | MTR_2g083870 | 85.714 | 42 | 6 | 0 | 3 | 44 | 87 | 128 | 1.45e-19 | 82.4 |
| MsG0780039770.01.T01 | MTR_1g099600 | 80.952 | 42 | 8 | 0 | 2 | 43 | 191 | 232 | 4.67e-18 | 79.0 |
| MsG0780039770.01.T01 | MTR_1g009613 | 74.419 | 43 | 11 | 0 | 2 | 44 | 268 | 310 | 1.93e-17 | 77.4 |
| MsG0780039770.01.T01 | MTR_4g127040 | 74.419 | 43 | 11 | 0 | 2 | 44 | 251 | 293 | 9.27e-17 | 75.5 |
| MsG0780039770.01.T01 | MTR_5g016610 | 72.093 | 43 | 12 | 0 | 2 | 44 | 205 | 247 | 1.54e-16 | 74.3 |
| MsG0780039770.01.T01 | MTR_1g070455 | 56.250 | 64 | 23 | 2 | 2 | 63 | 299 | 359 | 6.27e-16 | 73.6 |
| MsG0780039770.01.T01 | MTR_4g094908 | 67.442 | 43 | 14 | 0 | 2 | 44 | 272 | 314 | 3.83e-15 | 71.2 |
| MsG0780039770.01.T01 | MTR_3g108960 | 60.465 | 43 | 17 | 0 | 2 | 44 | 320 | 362 | 1.48e-13 | 67.0 |
| MsG0780039770.01.T01 | MTR_0117s0040 | 60.465 | 43 | 17 | 0 | 2 | 44 | 80 | 122 | 1.73e-13 | 65.9 |
| MsG0780039770.01.T01 | MTR_2g024350 | 57.143 | 42 | 18 | 0 | 3 | 44 | 15 | 56 | 1.69e-11 | 60.1 |
| MsG0780039770.01.T01 | MTR_2g024380 | 57.143 | 42 | 18 | 0 | 3 | 44 | 258 | 299 | 2.93e-11 | 60.5 |
| MsG0780039770.01.T01 | MTR_5g043880 | 59.524 | 42 | 16 | 1 | 3 | 44 | 131 | 171 | 4.04e-11 | 59.7 |
| MsG0780039770.01.T01 | MTR_8g067635 | 66.667 | 36 | 12 | 0 | 3 | 38 | 356 | 391 | 5.06e-11 | 59.7 |
| MsG0780039770.01.T01 | MTR_5g091390 | 57.143 | 42 | 17 | 1 | 3 | 44 | 164 | 204 | 5.23e-11 | 59.3 |
| MsG0780039770.01.T01 | MTR_3g090860 | 60.465 | 43 | 15 | 1 | 3 | 43 | 140 | 182 | 7.20e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039770.01.T01 | AT4G01250 | 46.552 | 174 | 47 | 6 | 2 | 141 | 134 | 295 | 8.25e-35 | 122 |
| MsG0780039770.01.T01 | AT2G34830 | 80.851 | 47 | 9 | 0 | 2 | 48 | 221 | 267 | 4.71e-21 | 88.2 |
| MsG0780039770.01.T01 | AT2G34830 | 80.851 | 47 | 9 | 0 | 2 | 48 | 230 | 276 | 5.00e-21 | 88.2 |
| MsG0780039770.01.T01 | AT1G29280 | 80.435 | 46 | 9 | 0 | 3 | 48 | 81 | 126 | 2.18e-20 | 84.3 |
| MsG0780039770.01.T01 | AT1G30650 | 83.721 | 43 | 7 | 0 | 2 | 44 | 223 | 265 | 4.23e-20 | 85.5 |
| MsG0780039770.01.T01 | AT5G52830 | 83.721 | 43 | 7 | 0 | 2 | 44 | 171 | 213 | 6.01e-20 | 84.3 |
| MsG0780039770.01.T01 | AT3G58710 | 83.333 | 42 | 7 | 0 | 3 | 44 | 78 | 119 | 5.76e-19 | 80.9 |
| MsG0780039770.01.T01 | AT3G58710 | 83.333 | 42 | 7 | 0 | 3 | 44 | 77 | 118 | 6.37e-19 | 80.9 |
| MsG0780039770.01.T01 | AT4G23550 | 63.934 | 61 | 20 | 1 | 3 | 63 | 147 | 205 | 1.41e-18 | 80.5 |
| MsG0780039770.01.T01 | AT5G45050 | 78.571 | 42 | 9 | 0 | 3 | 44 | 1048 | 1089 | 7.46e-18 | 79.3 |
| MsG0780039770.01.T01 | AT5G45050 | 78.571 | 42 | 9 | 0 | 3 | 44 | 1187 | 1228 | 7.52e-18 | 79.3 |
| MsG0780039770.01.T01 | AT5G45050 | 78.571 | 42 | 9 | 0 | 3 | 44 | 1159 | 1200 | 8.19e-18 | 79.3 |
| MsG0780039770.01.T01 | AT2G30590 | 59.091 | 66 | 23 | 2 | 2 | 67 | 319 | 380 | 9.50e-17 | 75.9 |
| MsG0780039770.01.T01 | AT4G31550 | 69.767 | 43 | 13 | 0 | 2 | 44 | 251 | 293 | 1.08e-15 | 72.8 |
| MsG0780039770.01.T01 | AT4G31550 | 69.767 | 43 | 13 | 0 | 2 | 44 | 252 | 294 | 1.10e-15 | 72.4 |
| MsG0780039770.01.T01 | AT2G23320 | 69.767 | 43 | 13 | 0 | 2 | 44 | 246 | 288 | 2.08e-15 | 71.6 |
| MsG0780039770.01.T01 | AT4G24240 | 69.767 | 43 | 13 | 0 | 2 | 44 | 287 | 329 | 5.00e-15 | 70.9 |
| MsG0780039770.01.T01 | AT2G24570 | 69.767 | 43 | 13 | 0 | 2 | 44 | 249 | 291 | 5.10e-15 | 70.9 |
| MsG0780039770.01.T01 | AT5G28650 | 67.442 | 43 | 14 | 0 | 2 | 44 | 268 | 310 | 3.60e-14 | 68.6 |
| MsG0780039770.01.T01 | AT3G04670 | 67.442 | 43 | 14 | 0 | 2 | 44 | 268 | 310 | 4.35e-14 | 68.2 |
| MsG0780039770.01.T01 | AT2G04880 | 61.905 | 42 | 15 | 1 | 3 | 44 | 290 | 330 | 4.71e-11 | 59.7 |
| MsG0780039770.01.T01 | AT2G04880 | 61.905 | 42 | 15 | 1 | 3 | 44 | 314 | 354 | 5.30e-11 | 59.7 |
Find 28 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGCTATTGCAATTCCTAAT+TGG | 0.183200 | 7:+68918677 | MsG0780039770.01.T01:CDS |
| TTTGAAAGTTTAGATGAATT+TGG | 0.224134 | 7:+68918634 | MsG0780039770.01.T01:CDS |
| TCAGCTTGAGGTGGTGTTAA+TGG | 0.224988 | 7:-68918439 | None:intergenic |
| GATGAAGAAGGAGATGAATT+TGG | 0.239015 | 7:+68918580 | MsG0780039770.01.T01:CDS |
| TGCTATTGCAATTCCTAATT+GGG | 0.291536 | 7:+68918678 | MsG0780039770.01.T01:CDS |
| GCACTATTAGCAACCCAATT+AGG | 0.295679 | 7:-68918691 | None:intergenic |
| GCTGCTGCTGCTACAGCTGC+TGG | 0.297450 | 7:+68918712 | MsG0780039770.01.T01:CDS |
| TATATGGGCATGGAGAAATA+TGG | 0.332062 | 7:+68918128 | None:intergenic |
| TCTTCACCTCCTGAGGTTGT+AGG | 0.351765 | 7:-68918493 | None:intergenic |
| GTCAGAAAGTAGAGAAGAAA+AGG | 0.360877 | 7:+68918540 | MsG0780039770.01.T01:CDS |
| ATTGCAATAGCAGAAGAAAA+TGG | 0.365837 | 7:-68918667 | None:intergenic |
| AGAAAAGGAAGATTCTTTGA+TGG | 0.372979 | 7:+68918555 | MsG0780039770.01.T01:CDS |
| TATAGATGTAGCAGTTCAAA+AGG | 0.420572 | 7:+68918290 | MsG0780039770.01.T01:CDS |
| TCTTTGTTGGTTTCAGCTTG+AGG | 0.464539 | 7:-68918451 | None:intergenic |
| TATGTTACTATGAACATTGT+TGG | 0.470318 | 7:-68918353 | None:intergenic |
| AACCTCTTCTTCACCTCCTG+AGG | 0.488504 | 7:-68918500 | None:intergenic |
| ATATGGGCATGGAGAAATAT+GGG | 0.492370 | 7:+68918129 | None:intergenic |
| CCCTCTTCTCCTACAACCTC+AGG | 0.495834 | 7:+68918484 | MsG0780039770.01.T01:CDS |
| GCTGCTGCTACAGCTGCTGG+TGG | 0.522446 | 7:+68918715 | MsG0780039770.01.T01:CDS |
| CCTGAGGTTGTAGGAGAAGA+GGG | 0.546404 | 7:-68918484 | None:intergenic |
| TCTTTGATGGATGATGAAGA+AGG | 0.562592 | 7:+68918568 | MsG0780039770.01.T01:CDS |
| TCCTGAGGTTGTAGGAGAAG+AGG | 0.567164 | 7:-68918485 | None:intergenic |
| GAAGAAGAGGTTCAAACTCA+AGG | 0.585892 | 7:+68918511 | MsG0780039770.01.T01:CDS |
| TAAAAGGGTCACCATATCCA+AGG | 0.597269 | 7:+68918162 | MsG0780039770.01.T01:CDS |
| TTGTTGGTTTCAGCTTGAGG+TGG | 0.614005 | 7:-68918448 | None:intergenic |
| ATAGATGTAGCAGTTCAAAA+GGG | 0.629154 | 7:+68918291 | MsG0780039770.01.T01:CDS |
| AACCTCAGGAGGTGAAGAAG+AGG | 0.630119 | 7:+68918498 | MsG0780039770.01.T01:CDS |
| TCTTCTCCTACAACCTCAGG+AGG | 0.702219 | 7:+68918487 | MsG0780039770.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAAAATTGAATTTTTACCT+TGG | - | Chr7:68918182-68918201 | None:intergenic | 15.0% |
| !!! | AATTTTGTTTTGTGATTTAT+AGG | + | Chr7:68918261-68918280 | MsG0780039770.01.T01:intron | 15.0% |
| !!! | ATTTTGTTTTGTGATTTATA+GGG | + | Chr7:68918262-68918281 | MsG0780039770.01.T01:intron | 15.0% |
| !! | TTTGAAAGTTTAGATGAATT+TGG | + | Chr7:68918634-68918653 | MsG0780039770.01.T01:CDS | 20.0% |
| !!! | TTTTGTTTTGTGATTTATAG+GGG | + | Chr7:68918263-68918282 | MsG0780039770.01.T01:intron | 20.0% |
| ! | TATGTTACTATGAACATTGT+TGG | - | Chr7:68918356-68918375 | None:intergenic | 25.0% |
| !! | TTGAATTTTTACCTTGGATA+TGG | - | Chr7:68918176-68918195 | None:intergenic | 25.0% |
| AGAAAAGGAAGATTCTTTGA+TGG | + | Chr7:68918555-68918574 | MsG0780039770.01.T01:CDS | 30.0% | |
| ATAGATGTAGCAGTTCAAAA+GGG | + | Chr7:68918291-68918310 | MsG0780039770.01.T01:CDS | 30.0% | |
| ATGGGCAAAAACCTATAAAA+GGG | + | Chr7:68918147-68918166 | MsG0780039770.01.T01:CDS | 30.0% | |
| ATTGCAATAGCAGAAGAAAA+TGG | - | Chr7:68918670-68918689 | None:intergenic | 30.0% | |
| TATAGATGTAGCAGTTCAAA+AGG | + | Chr7:68918290-68918309 | MsG0780039770.01.T01:CDS | 30.0% | |
| TATGGGCAAAAACCTATAAA+AGG | + | Chr7:68918146-68918165 | MsG0780039770.01.T01:CDS | 30.0% | |
| TGCTATTGCAATTCCTAATT+GGG | + | Chr7:68918678-68918697 | MsG0780039770.01.T01:CDS | 30.0% | |
| CTGCTATTGCAATTCCTAAT+TGG | + | Chr7:68918677-68918696 | MsG0780039770.01.T01:CDS | 35.0% | |
| GATGAAGAAGGAGATGAATT+TGG | + | Chr7:68918580-68918599 | MsG0780039770.01.T01:CDS | 35.0% | |
| GTCAGAAAGTAGAGAAGAAA+AGG | + | Chr7:68918540-68918559 | MsG0780039770.01.T01:CDS | 35.0% | |
| ! | TCTTTGATGGATGATGAAGA+AGG | + | Chr7:68918568-68918587 | MsG0780039770.01.T01:CDS | 35.0% |
| ACACACAAAAACTCTCTTGC+TGG | + | Chr7:68918400-68918419 | MsG0780039770.01.T01:intron | 40.0% | |
| GAAGAAGAGGTTCAAACTCA+AGG | + | Chr7:68918511-68918530 | MsG0780039770.01.T01:CDS | 40.0% | |
| GCACTATTAGCAACCCAATT+AGG | - | Chr7:68918694-68918713 | None:intergenic | 40.0% | |
| TAAAAGGGTCACCATATCCA+AGG | + | Chr7:68918162-68918181 | MsG0780039770.01.T01:CDS | 40.0% | |
| ! | GCAAGAGAGTTTTTGTGTGT+AGG | - | Chr7:68918400-68918419 | None:intergenic | 40.0% |
| ! | GGATATGGTGACCCTTTTAT+AGG | - | Chr7:68918161-68918180 | None:intergenic | 40.0% |
| !! | GGGTTTTGTAGGATCTTTGT+TGG | - | Chr7:68918467-68918486 | None:intergenic | 40.0% |
| !! | TCTTTGTTGGTTTCAGCTTG+AGG | - | Chr7:68918454-68918473 | None:intergenic | 40.0% |
| ! | GAGTTTTTGTGTGTAGGAGC+AGG | - | Chr7:68918394-68918413 | None:intergenic | 45.0% |
| ! | TCAGCTTGAGGTGGTGTTAA+TGG | - | Chr7:68918442-68918461 | None:intergenic | 45.0% |
| !! | ACTCTCTTGCTGGTTCAACA+AGG | + | Chr7:68918410-68918429 | MsG0780039770.01.T01:intron | 45.0% |
| !! | GTAGGAGAAGAGGGTTTTGT+AGG | - | Chr7:68918478-68918497 | None:intergenic | 45.0% |
| !! | TTGTTGGTTTCAGCTTGAGG+TGG | - | Chr7:68918451-68918470 | None:intergenic | 45.0% |
| AACCTCAGGAGGTGAAGAAG+AGG | + | Chr7:68918498-68918517 | MsG0780039770.01.T01:CDS | 50.0% | |
| AACCTCTTCTTCACCTCCTG+AGG | - | Chr7:68918503-68918522 | None:intergenic | 50.0% | |
| CCTGAGGTTGTAGGAGAAGA+GGG | - | Chr7:68918487-68918506 | None:intergenic | 50.0% | |
| TCCTGAGGTTGTAGGAGAAG+AGG | - | Chr7:68918488-68918507 | None:intergenic | 50.0% | |
| TCTTCACCTCCTGAGGTTGT+AGG | - | Chr7:68918496-68918515 | None:intergenic | 50.0% | |
| TCTTCTCCTACAACCTCAGG+AGG | + | Chr7:68918487-68918506 | MsG0780039770.01.T01:CDS | 50.0% | |
| CCCTCTTCTCCTACAACCTC+AGG | + | Chr7:68918484-68918503 | MsG0780039770.01.T01:CDS | 55.0% | |
| GCTGCTGCTACAGCTGCTGG+TGG | + | Chr7:68918715-68918734 | MsG0780039770.01.T01:CDS | 65.0% | |
| GCTGCTGCTGCTACAGCTGC+TGG | + | Chr7:68918712-68918731 | MsG0780039770.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 68918137 | 68918744 | 68918137 | ID=MsG0780039770.01;Name=MsG0780039770.01 |
| Chr7 | mRNA | 68918137 | 68918744 | 68918137 | ID=MsG0780039770.01.T01;Parent=MsG0780039770.01;Name=MsG0780039770.01.T01;_AED=0.48;_eAED=0.49;_QI=0|0|0|1|1|1|3|0|146 |
| Chr7 | exon | 68918137 | 68918183 | 68918137 | ID=MsG0780039770.01.T01:exon:12238;Parent=MsG0780039770.01.T01 |
| Chr7 | exon | 68918283 | 68918367 | 68918283 | ID=MsG0780039770.01.T01:exon:12239;Parent=MsG0780039770.01.T01 |
| Chr7 | exon | 68918436 | 68918744 | 68918436 | ID=MsG0780039770.01.T01:exon:12240;Parent=MsG0780039770.01.T01 |
| Chr7 | CDS | 68918137 | 68918183 | 68918137 | ID=MsG0780039770.01.T01:cds;Parent=MsG0780039770.01.T01 |
| Chr7 | CDS | 68918283 | 68918367 | 68918283 | ID=MsG0780039770.01.T01:cds;Parent=MsG0780039770.01.T01 |
| Chr7 | CDS | 68918436 | 68918744 | 68918436 | ID=MsG0780039770.01.T01:cds;Parent=MsG0780039770.01.T01 |
| Gene Sequence |
| Protein sequence |
