Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039933.01.T01 | XP_003624327.1 | 95.614 | 114 | 5 | 0 | 1 | 114 | 947 | 1060 | 3.90E-66 | 226 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039933.01.T01 | Q8VZG8 | 51.724 | 87 | 37 | 2 | 1 | 86 | 955 | 1037 | 3.70E-17 | 79 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039933.01.T01 | G7KU95 | 95.614 | 114 | 5 | 0 | 1 | 114 | 947 | 1060 | 1.86e-66 | 226 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039933.01.T01 | MTR_7g081780 | 95.614 | 114 | 5 | 0 | 1 | 114 | 947 | 1060 | 4.72e-70 | 226 |
| MsG0780039933.01.T01 | MTR_7g081480 | 86.607 | 112 | 15 | 0 | 1 | 112 | 949 | 1060 | 9.63e-62 | 203 |
| MsG0780039933.01.T01 | MTR_7g481630 | 76.106 | 113 | 26 | 1 | 1 | 112 | 80 | 192 | 9.00e-57 | 174 |
| MsG0780039933.01.T01 | MTR_7g481590 | 76.106 | 113 | 26 | 1 | 1 | 112 | 203 | 315 | 7.03e-55 | 173 |
| MsG0780039933.01.T01 | MTR_7g081570 | 76.991 | 113 | 25 | 1 | 1 | 112 | 939 | 1051 | 1.40e-52 | 177 |
| MsG0780039933.01.T01 | MTR_7g081410 | 75.221 | 113 | 27 | 1 | 1 | 112 | 826 | 938 | 1.71e-51 | 174 |
| MsG0780039933.01.T01 | MTR_7g081410 | 75.221 | 113 | 27 | 1 | 1 | 112 | 946 | 1058 | 1.95e-51 | 174 |
| MsG0780039933.01.T01 | MTR_5g085700 | 53.571 | 112 | 52 | 0 | 2 | 113 | 921 | 1032 | 2.91e-31 | 116 |
| MsG0780039933.01.T01 | MTR_7g081720 | 52.632 | 114 | 52 | 1 | 1 | 112 | 964 | 1077 | 2.24e-30 | 114 |
| MsG0780039933.01.T01 | MTR_8g445960 | 49.000 | 100 | 50 | 1 | 1 | 99 | 63 | 162 | 2.12e-28 | 101 |
| MsG0780039933.01.T01 | MTR_8g044940 | 49.000 | 100 | 50 | 1 | 1 | 99 | 63 | 162 | 2.12e-28 | 101 |
| MsG0780039933.01.T01 | MTR_1g039080 | 52.525 | 99 | 46 | 1 | 1 | 98 | 192 | 290 | 1.44e-27 | 102 |
| MsG0780039933.01.T01 | MTR_1g039310 | 48.246 | 114 | 55 | 2 | 3 | 112 | 1051 | 1164 | 1.29e-26 | 103 |
| MsG0780039933.01.T01 | MTR_1g038890 | 52.525 | 99 | 46 | 1 | 1 | 98 | 1033 | 1131 | 2.58e-26 | 102 |
| MsG0780039933.01.T01 | MTR_7g045510 | 52.174 | 92 | 42 | 1 | 2 | 93 | 1091 | 1180 | 9.00e-24 | 95.1 |
| MsG0780039933.01.T01 | MTR_7g039330 | 52.174 | 92 | 42 | 1 | 2 | 93 | 1189 | 1278 | 1.47e-23 | 94.4 |
| MsG0780039933.01.T01 | MTR_7g007540 | 44.737 | 114 | 58 | 2 | 1 | 113 | 1 | 110 | 1.82e-23 | 87.8 |
| MsG0780039933.01.T01 | MTR_7g007560 | 46.491 | 114 | 56 | 2 | 1 | 113 | 653 | 762 | 2.95e-23 | 93.6 |
| MsG0780039933.01.T01 | MTR_7g009940 | 44.248 | 113 | 58 | 2 | 3 | 114 | 700 | 808 | 2.68e-22 | 90.9 |
| MsG0780039933.01.T01 | MTR_7g045710 | 48.936 | 94 | 43 | 1 | 2 | 95 | 972 | 1060 | 1.38e-21 | 88.6 |
| MsG0780039933.01.T01 | MTR_1g039240 | 47.423 | 97 | 47 | 1 | 1 | 93 | 1058 | 1154 | 2.37e-21 | 88.2 |
| MsG0780039933.01.T01 | MTR_0400s0040 | 47.423 | 97 | 47 | 1 | 1 | 93 | 1058 | 1154 | 3.05e-21 | 87.8 |
| MsG0780039933.01.T01 | MTR_0365s0030 | 47.423 | 97 | 47 | 1 | 1 | 93 | 1058 | 1154 | 3.05e-21 | 87.8 |
| MsG0780039933.01.T01 | MTR_7g446160 | 51.579 | 95 | 43 | 2 | 1 | 93 | 887 | 980 | 3.11e-21 | 87.8 |
| MsG0780039933.01.T01 | MTR_0400s0030 | 48.485 | 99 | 50 | 1 | 1 | 98 | 791 | 889 | 3.72e-21 | 87.4 |
| MsG0780039933.01.T01 | MTR_0365s0020 | 48.485 | 99 | 50 | 1 | 1 | 98 | 791 | 889 | 3.72e-21 | 87.4 |
| MsG0780039933.01.T01 | MTR_7g051040 | 42.609 | 115 | 57 | 2 | 1 | 108 | 843 | 955 | 6.89e-21 | 86.7 |
| MsG0780039933.01.T01 | MTR_7g050990 | 49.495 | 99 | 42 | 3 | 1 | 93 | 819 | 915 | 8.42e-21 | 86.7 |
| MsG0780039933.01.T01 | MTR_7g007630 | 44.737 | 114 | 58 | 2 | 1 | 113 | 666 | 775 | 9.35e-21 | 86.3 |
| MsG0780039933.01.T01 | MTR_7g045860 | 48.454 | 97 | 46 | 2 | 2 | 97 | 984 | 1077 | 2.14e-20 | 85.5 |
| MsG0780039933.01.T01 | MTR_1g040525 | 48.958 | 96 | 43 | 3 | 1 | 91 | 767 | 861 | 2.39e-20 | 85.1 |
| MsG0780039933.01.T01 | MTR_7g009970 | 43.966 | 116 | 56 | 3 | 1 | 113 | 669 | 778 | 3.34e-20 | 84.7 |
| MsG0780039933.01.T01 | MTR_7g407090 | 45.614 | 114 | 57 | 2 | 1 | 113 | 651 | 760 | 4.55e-20 | 84.3 |
| MsG0780039933.01.T01 | MTR_1g040545 | 50.000 | 96 | 42 | 3 | 1 | 91 | 907 | 1001 | 4.72e-20 | 84.3 |
| MsG0780039933.01.T01 | MTR_7g407130 | 44.248 | 113 | 58 | 2 | 1 | 112 | 696 | 804 | 7.07e-20 | 84.0 |
| MsG0780039933.01.T01 | MTR_1g040615 | 47.423 | 97 | 47 | 3 | 1 | 93 | 2024 | 2120 | 2.59e-19 | 82.4 |
| MsG0780039933.01.T01 | MTR_1g040615 | 44.545 | 110 | 49 | 5 | 1 | 103 | 1025 | 1129 | 8.40e-19 | 80.9 |
| MsG0780039933.01.T01 | MTR_1g039220 | 48.421 | 95 | 45 | 3 | 1 | 91 | 1032 | 1126 | 2.76e-19 | 82.0 |
| MsG0780039933.01.T01 | MTR_7g007620 | 46.491 | 114 | 56 | 2 | 1 | 113 | 620 | 729 | 9.29e-19 | 80.5 |
| MsG0780039933.01.T01 | MTR_7g013680 | 42.017 | 119 | 56 | 4 | 1 | 114 | 619 | 729 | 2.12e-18 | 79.7 |
| MsG0780039933.01.T01 | MTR_7g010000 | 42.241 | 116 | 58 | 3 | 1 | 113 | 746 | 855 | 5.56e-18 | 78.6 |
| MsG0780039933.01.T01 | MTR_4g037720 | 44.086 | 93 | 48 | 1 | 1 | 93 | 848 | 936 | 5.93e-18 | 78.2 |
| MsG0780039933.01.T01 | MTR_4g037015 | 47.368 | 95 | 42 | 3 | 1 | 93 | 777 | 865 | 1.19e-17 | 77.4 |
| MsG0780039933.01.T01 | MTR_7g028432 | 47.368 | 95 | 42 | 3 | 1 | 93 | 853 | 941 | 1.51e-17 | 77.0 |
| MsG0780039933.01.T01 | MTR_7g096980 | 41.837 | 98 | 54 | 2 | 1 | 97 | 1108 | 1203 | 3.74e-17 | 76.3 |
| MsG0780039933.01.T01 | MTR_8g047230 | 51.087 | 92 | 40 | 2 | 1 | 91 | 875 | 962 | 1.02e-16 | 74.7 |
| MsG0780039933.01.T01 | MTR_0491s0030 | 44.737 | 114 | 58 | 2 | 1 | 113 | 704 | 813 | 2.31e-16 | 73.9 |
| MsG0780039933.01.T01 | MTR_8g047160 | 52.874 | 87 | 36 | 2 | 1 | 86 | 531 | 613 | 2.64e-16 | 73.6 |
| MsG0780039933.01.T01 | MTR_7g013350 | 43.810 | 105 | 46 | 4 | 1 | 100 | 295 | 391 | 1.08e-15 | 71.6 |
| MsG0780039933.01.T01 | MTR_8g047210 | 44.737 | 114 | 58 | 2 | 1 | 113 | 768 | 877 | 4.15e-15 | 70.1 |
| MsG0780039933.01.T01 | MTR_8g047220 | 44.737 | 114 | 58 | 2 | 1 | 113 | 831 | 940 | 4.42e-15 | 70.1 |
| MsG0780039933.01.T01 | MTR_7g446190 | 45.238 | 84 | 39 | 2 | 1 | 80 | 815 | 895 | 7.22e-14 | 66.6 |
| MsG0780039933.01.T01 | MTR_7g022160 | 35.455 | 110 | 66 | 2 | 1 | 107 | 909 | 1016 | 9.85e-14 | 66.2 |
| MsG0780039933.01.T01 | MTR_2g070020 | 38.000 | 100 | 51 | 2 | 1 | 93 | 987 | 1082 | 1.15e-13 | 66.2 |
| MsG0780039933.01.T01 | MTR_7g010010 | 37.069 | 116 | 45 | 3 | 1 | 113 | 616 | 706 | 5.62e-13 | 63.9 |
| MsG0780039933.01.T01 | MTR_7g022050 | 34.545 | 110 | 67 | 2 | 1 | 107 | 534 | 641 | 6.12e-13 | 63.9 |
| MsG0780039933.01.T01 | MTR_3g093710 | 33.333 | 105 | 59 | 3 | 2 | 99 | 517 | 617 | 1.61e-11 | 60.1 |
| MsG0780039933.01.T01 | MTR_3g093930 | 35.294 | 102 | 61 | 3 | 1 | 99 | 879 | 978 | 2.62e-11 | 59.3 |
| MsG0780039933.01.T01 | MTR_3g047890 | 36.036 | 111 | 51 | 4 | 3 | 102 | 360 | 461 | 2.63e-11 | 59.3 |
| MsG0780039933.01.T01 | MTR_7g111690 | 37.500 | 104 | 55 | 2 | 3 | 100 | 374 | 473 | 3.17e-11 | 58.9 |
| MsG0780039933.01.T01 | MTR_7g007580 | 39.048 | 105 | 59 | 2 | 10 | 113 | 3 | 103 | 4.52e-11 | 55.8 |
| MsG0780039933.01.T01 | MTR_1g080440 | 31.000 | 100 | 66 | 1 | 1 | 97 | 888 | 987 | 6.34e-11 | 58.2 |
| MsG0780039933.01.T01 | MTR_1g069470 | 35.644 | 101 | 59 | 3 | 1 | 97 | 882 | 980 | 6.52e-11 | 58.2 |
| MsG0780039933.01.T01 | MTR_1g047670 | 36.000 | 100 | 55 | 3 | 1 | 93 | 989 | 1086 | 7.02e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039933.01.T01 | AT4G08850 | 51.724 | 87 | 37 | 2 | 1 | 86 | 955 | 1037 | 3.77e-18 | 79.0 |
| MsG0780039933.01.T01 | AT1G35710 | 44.944 | 89 | 39 | 3 | 1 | 86 | 1031 | 1112 | 2.39e-14 | 68.2 |
| MsG0780039933.01.T01 | AT3G28040 | 34.951 | 103 | 62 | 2 | 1 | 100 | 908 | 1008 | 4.69e-12 | 61.6 |
| MsG0780039933.01.T01 | AT1G17230 | 38.000 | 100 | 51 | 2 | 1 | 93 | 977 | 1072 | 4.72e-12 | 61.6 |
| MsG0780039933.01.T01 | AT1G17230 | 38.000 | 100 | 51 | 2 | 1 | 93 | 977 | 1072 | 4.85e-12 | 61.6 |
| MsG0780039933.01.T01 | AT1G09440 | 36.842 | 95 | 54 | 2 | 3 | 93 | 336 | 428 | 5.59e-12 | 61.2 |
| MsG0780039933.01.T01 | AT1G09440 | 36.842 | 95 | 54 | 2 | 3 | 93 | 336 | 428 | 5.59e-12 | 61.2 |
| MsG0780039933.01.T01 | AT4G25390 | 37.755 | 98 | 51 | 2 | 3 | 93 | 530 | 624 | 1.27e-11 | 60.5 |
| MsG0780039933.01.T01 | AT5G51770 | 35.238 | 105 | 57 | 3 | 3 | 99 | 528 | 629 | 1.60e-11 | 60.1 |
| MsG0780039933.01.T01 | AT1G07650 | 34.906 | 106 | 62 | 3 | 3 | 104 | 645 | 747 | 2.29e-11 | 59.7 |
| MsG0780039933.01.T01 | AT1G07650 | 34.906 | 106 | 62 | 3 | 3 | 104 | 858 | 960 | 2.47e-11 | 59.7 |
| MsG0780039933.01.T01 | AT1G07650 | 34.906 | 106 | 62 | 3 | 3 | 104 | 864 | 966 | 2.52e-11 | 59.3 |
| MsG0780039933.01.T01 | AT1G07650 | 34.906 | 106 | 62 | 3 | 3 | 104 | 832 | 934 | 2.74e-11 | 59.3 |
| MsG0780039933.01.T01 | AT5G48940 | 36.458 | 96 | 54 | 2 | 1 | 93 | 972 | 1063 | 2.76e-11 | 59.3 |
| MsG0780039933.01.T01 | AT4G31110 | 33.645 | 107 | 63 | 3 | 5 | 105 | 612 | 716 | 5.85e-11 | 58.5 |
| MsG0780039933.01.T01 | AT4G31110 | 33.645 | 107 | 63 | 3 | 5 | 105 | 634 | 738 | 5.86e-11 | 58.5 |
| MsG0780039933.01.T01 | AT2G39660 | 37.383 | 107 | 56 | 4 | 3 | 104 | 256 | 356 | 8.14e-11 | 57.8 |
Find 23 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTGCTTTGGAAATCATAA+AGG | 0.335311 | 7:+70605806 | MsG0780039933.01.T01:CDS |
| AAATGTGATGTATACAGTTT+TGG | 0.382117 | 7:+70605780 | MsG0780039933.01.T01:CDS |
| AAGATAACCTCCTCATCAAT+TGG | 0.413575 | 7:-70605942 | None:intergenic |
| ATTGGTTTCATAACTATTTG+AGG | 0.415691 | 7:-70605924 | None:intergenic |
| CTTGCTTTGGAAATCATAAA+GGG | 0.419654 | 7:+70605807 | MsG0780039933.01.T01:CDS |
| TGGAGCAGGAAAATCACCTT+TGG | 0.448518 | 7:+70606043 | MsG0780039933.01.T01:CDS |
| TTAGCCAATGGCCTTGTAGA+TGG | 0.459178 | 7:-70605867 | None:intergenic |
| GTTGGACGAGAACGTGGCTC+TGG | 0.460252 | 7:-70605996 | None:intergenic |
| CAACTTCACACGATCAAACT+AGG | 0.475214 | 7:+70606071 | MsG0780039933.01.T01:CDS |
| ATCATAAAGGGAGAACATCC+TGG | 0.483511 | 7:+70605819 | MsG0780039933.01.T01:CDS |
| TATAATGAATCGAGATCTCC+AGG | 0.511431 | 7:-70605837 | None:intergenic |
| GATCAAGTATGTAAGATGCT+TGG | 0.514611 | 7:+70606023 | MsG0780039933.01.T01:CDS |
| GTCACCATCTACAAGGCCAT+TGG | 0.522420 | 7:+70605863 | MsG0780039933.01.T01:CDS |
| GTATGTAAGATGCTTGGAGC+AGG | 0.525016 | 7:+70606029 | MsG0780039933.01.T01:CDS |
| CATCTTACATACTTGATCCA+TGG | 0.528171 | 7:-70606018 | None:intergenic |
| TATATTTGTCACCATCTACA+AGG | 0.550499 | 7:+70605856 | MsG0780039933.01.T01:CDS |
| TTACATACTTGATCCATGGT+TGG | 0.555375 | 7:-70606014 | None:intergenic |
| TATGAAACCAATTGATGAGG+AGG | 0.585223 | 7:+70605935 | MsG0780039933.01.T01:CDS |
| GCCACGTTCTCGTCCAACCA+TGG | 0.605254 | 7:+70606001 | MsG0780039933.01.T01:CDS |
| TCCATGGTTGGACGAGAACG+TGG | 0.627661 | 7:-70606002 | None:intergenic |
| AATAATGTATCATTAGCCAA+TGG | 0.628834 | 7:-70605879 | None:intergenic |
| AGTTATGAAACCAATTGATG+AGG | 0.632329 | 7:+70605932 | MsG0780039933.01.T01:CDS |
| GTGTGAAGTTGATCTTCCAA+AGG | 0.654089 | 7:-70606059 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATAATGTATCATTAGCCAA+TGG | - | Chr7:70605882-70605901 | None:intergenic | 25.0% |
| !! | AAATGTGATGTATACAGTTT+TGG | + | Chr7:70605780-70605799 | MsG0780039933.01.T01:CDS | 25.0% |
| !! | ATTGGTTTCATAACTATTTG+AGG | - | Chr7:70605927-70605946 | None:intergenic | 25.0% |
| AGTTATGAAACCAATTGATG+AGG | + | Chr7:70605932-70605951 | MsG0780039933.01.T01:CDS | 30.0% | |
| TATATTTGTCACCATCTACA+AGG | + | Chr7:70605856-70605875 | MsG0780039933.01.T01:CDS | 30.0% | |
| !! | ACTTGCTTTGGAAATCATAA+AGG | + | Chr7:70605806-70605825 | MsG0780039933.01.T01:CDS | 30.0% |
| !! | CTTGCTTTGGAAATCATAAA+GGG | + | Chr7:70605807-70605826 | MsG0780039933.01.T01:CDS | 30.0% |
| AAGATAACCTCCTCATCAAT+TGG | - | Chr7:70605945-70605964 | None:intergenic | 35.0% | |
| CATCTTACATACTTGATCCA+TGG | - | Chr7:70606021-70606040 | None:intergenic | 35.0% | |
| GATCAAGTATGTAAGATGCT+TGG | + | Chr7:70606023-70606042 | MsG0780039933.01.T01:CDS | 35.0% | |
| TATAATGAATCGAGATCTCC+AGG | - | Chr7:70605840-70605859 | None:intergenic | 35.0% | |
| TATGAAACCAATTGATGAGG+AGG | + | Chr7:70605935-70605954 | MsG0780039933.01.T01:CDS | 35.0% | |
| TTACATACTTGATCCATGGT+TGG | - | Chr7:70606017-70606036 | None:intergenic | 35.0% | |
| ATCATAAAGGGAGAACATCC+TGG | + | Chr7:70605819-70605838 | MsG0780039933.01.T01:CDS | 40.0% | |
| CAACTTCACACGATCAAACT+AGG | + | Chr7:70606071-70606090 | MsG0780039933.01.T01:CDS | 40.0% | |
| ! | GTGTGAAGTTGATCTTCCAA+AGG | - | Chr7:70606062-70606081 | None:intergenic | 40.0% |
| !!! | CAGTTTTGGAGTACTTGCTT+TGG | + | Chr7:70605794-70605813 | MsG0780039933.01.T01:CDS | 40.0% |
| GTATGTAAGATGCTTGGAGC+AGG | + | Chr7:70606029-70606048 | MsG0780039933.01.T01:CDS | 45.0% | |
| TGGAGCAGGAAAATCACCTT+TGG | + | Chr7:70606043-70606062 | MsG0780039933.01.T01:CDS | 45.0% | |
| !! | TTAGCCAATGGCCTTGTAGA+TGG | - | Chr7:70605870-70605889 | None:intergenic | 45.0% |
| GTCACCATCTACAAGGCCAT+TGG | + | Chr7:70605863-70605882 | MsG0780039933.01.T01:CDS | 50.0% | |
| TCCATGGTTGGACGAGAACG+TGG | - | Chr7:70606005-70606024 | None:intergenic | 55.0% | |
| GCCACGTTCTCGTCCAACCA+TGG | + | Chr7:70606001-70606020 | MsG0780039933.01.T01:CDS | 60.0% | |
| GTTGGACGAGAACGTGGCTC+TGG | - | Chr7:70605999-70606018 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 70605765 | 70606109 | 70605765 | ID=MsG0780039933.01;Name=MsG0780039933.01 |
| Chr7 | mRNA | 70605765 | 70606109 | 70605765 | ID=MsG0780039933.01.T01;Parent=MsG0780039933.01;Name=MsG0780039933.01.T01;_AED=0.32;_eAED=0.32;_QI=0|-1|0|1|-1|0|1|0|114 |
| Chr7 | exon | 70605765 | 70606109 | 70605765 | ID=MsG0780039933.01.T01:exon:13437;Parent=MsG0780039933.01.T01 |
| Chr7 | CDS | 70605765 | 70606109 | 70605765 | ID=MsG0780039933.01.T01:cds;Parent=MsG0780039933.01.T01 |
| Gene Sequence |
| Protein sequence |
