AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040000.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040000.01.T01 MTR_7g083360 86.014 143 20 0 1 143 1 143 4.27e-95 273
MsG0780040000.01.T01 MTR_7g083330 63.043 184 60 3 3 178 11 194 1.54e-80 237
MsG0780040000.01.T01 MTR_7g083370 79.259 135 28 0 1 135 1 135 3.47e-78 229
MsG0780040000.01.T01 MTR_3g088110 34.868 152 89 5 6 154 3 147 6.11e-22 90.1
MsG0780040000.01.T01 MTR_3g109340 35.338 133 80 5 7 135 19 149 2.75e-21 89.4
MsG0780040000.01.T01 MTR_3g109340 35.338 133 80 5 7 135 19 149 2.78e-21 89.4
MsG0780040000.01.T01 MTR_6g032770 37.692 130 75 4 8 133 16 143 4.61e-21 89.0
MsG0780040000.01.T01 MTR_7g085260 36.364 132 76 5 8 133 15 144 3.09e-20 86.7
MsG0780040000.01.T01 MTR_7g085220 31.544 149 89 4 7 144 5 151 1.60e-19 84.3
MsG0780040000.01.T01 MTR_2157s0010 33.088 136 84 4 3 133 1 134 4.86e-19 82.0
MsG0780040000.01.T01 MTR_4g081870 31.613 155 96 6 4 154 3 151 5.01e-19 82.0
MsG0780040000.01.T01 MTR_1g008740 35.821 134 79 5 7 135 10 141 8.53e-19 82.4
MsG0780040000.01.T01 MTR_1g008740 35.821 134 79 5 7 135 24 155 8.76e-19 82.4
MsG0780040000.01.T01 MTR_8g059170 31.206 141 88 5 6 143 10 144 9.77e-19 82.0
MsG0780040000.01.T01 MTR_2g080010 32.353 136 86 4 2 133 7 140 1.11e-18 82.0
MsG0780040000.01.T01 MTR_3g096920 33.088 136 84 4 6 138 3 134 1.50e-18 81.3
MsG0780040000.01.T01 MTR_4g089135 34.375 128 78 4 10 133 15 140 1.54e-18 81.6
MsG0780040000.01.T01 MTR_2g079990 31.206 141 88 5 6 143 12 146 1.80e-18 81.6
MsG0780040000.01.T01 MTR_2g079990 31.206 141 88 5 6 143 12 146 2.39e-18 81.6
MsG0780040000.01.T01 MTR_6g084430 35.385 130 78 5 7 133 2 128 2.75e-18 80.9
MsG0780040000.01.T01 MTR_3g116070 34.132 167 91 8 7 167 20 173 2.81e-18 80.9
MsG0780040000.01.T01 MTR_2g064090 28.804 184 108 5 6 168 2 183 2.91e-18 80.9
MsG0780040000.01.T01 MTR_3g096920 33.088 136 84 4 6 138 3 134 3.15e-18 80.5
MsG0780040000.01.T01 MTR_7g005280 34.586 133 78 6 5 133 10 137 3.80e-18 79.3
MsG0780040000.01.T01 MTR_8g094580 32.090 134 84 4 8 138 5 134 5.16e-18 79.7
MsG0780040000.01.T01 MTR_5g076850 33.588 131 83 3 6 133 2 131 1.13e-17 80.1
MsG0780040000.01.T01 MTR_2g078700 33.594 128 79 5 10 133 23 148 1.75e-17 79.0
MsG0780040000.01.T01 MTR_5g041940 31.126 151 94 6 8 154 1 145 2.14e-17 77.4
MsG0780040000.01.T01 MTR_6g012670 33.083 133 83 4 4 133 14 143 2.41e-17 78.2
MsG0780040000.01.T01 MTR_5g040420 32.374 139 87 5 7 141 12 147 4.37e-17 77.8
MsG0780040000.01.T01 MTR_3g064580 32.824 131 84 3 6 133 2 131 7.50e-17 77.8
MsG0780040000.01.T01 MTR_7g011120 32.692 156 99 5 3 155 1 153 1.38e-16 75.9
MsG0780040000.01.T01 MTR_2g068880 34.532 139 81 6 3 133 14 150 1.62e-16 75.9
MsG0780040000.01.T01 MTR_4g108760 32.308 130 82 5 7 133 14 140 1.63e-16 76.3
MsG0780040000.01.T01 MTR_6g477900 33.333 129 82 3 8 133 4 131 2.02e-16 74.7
MsG0780040000.01.T01 MTR_3g435150 32.308 130 82 5 7 133 13 139 2.19e-16 75.5
MsG0780040000.01.T01 MTR_3g070030 35.075 134 81 5 3 133 12 142 2.23e-16 75.9
MsG0780040000.01.T01 MTR_8g099750 30.370 135 86 4 3 133 7 137 3.66e-16 73.6
MsG0780040000.01.T01 MTR_6g011860 31.852 135 87 3 9 140 14 146 4.97e-16 75.1
MsG0780040000.01.T01 MTR_2g062730 31.387 137 90 3 1 133 1 137 5.29e-16 74.7
MsG0780040000.01.T01 MTR_7g011130 33.582 134 83 5 3 133 12 142 5.77e-16 74.7
MsG0780040000.01.T01 MTR_2g093810 31.333 150 93 6 10 154 14 158 5.78e-16 74.3
MsG0780040000.01.T01 MTR_5g090970 34.091 132 79 5 7 133 16 144 6.36e-16 74.3
MsG0780040000.01.T01 MTR_5g090970 34.091 132 78 5 7 133 16 143 1.14e-15 73.6
MsG0780040000.01.T01 MTR_8g467490 31.884 138 87 5 7 140 12 146 1.43e-15 73.6
MsG0780040000.01.T01 MTR_2g068920 34.532 139 81 6 2 133 14 149 1.45e-15 72.8
MsG0780040000.01.T01 MTR_1g090720 32.283 127 82 3 10 133 42 167 1.99e-15 73.6
MsG0780040000.01.T01 MTR_3g096140 32.836 134 85 4 3 133 1 132 2.37e-15 72.0
MsG0780040000.01.T01 MTR_7g100990 30.827 133 86 4 5 133 6 136 3.70e-15 72.0
MsG0780040000.01.T01 MTR_3g096140 32.836 134 85 4 3 133 1 132 6.74e-15 71.6
MsG0780040000.01.T01 MTR_1g097300 31.746 126 82 3 13 135 22 146 7.03e-15 72.0
MsG0780040000.01.T01 MTR_1g069805 28.402 169 108 6 1 162 1 163 1.02e-14 70.5
MsG0780040000.01.T01 MTR_4g035590 29.630 189 110 8 1 172 1 183 3.55e-14 69.7
MsG0780040000.01.T01 MTR_5g021710 32.031 128 82 4 10 133 8 134 3.59e-14 69.7
MsG0780040000.01.T01 MTR_4g036030 31.250 128 83 4 10 133 7 133 3.68e-14 69.3
MsG0780040000.01.T01 MTR_7g097090 31.387 137 88 4 1 133 1 135 3.84e-14 68.9
MsG0780040000.01.T01 MTR_5g069030 34.646 127 79 3 10 133 25 150 7.45e-14 68.9
MsG0780040000.01.T01 MTR_8g076110 31.250 128 83 4 10 133 6 132 9.09e-14 68.2
MsG0780040000.01.T01 MTR_2g064470 28.472 144 97 4 3 144 4 143 1.03e-13 67.8
MsG0780040000.01.T01 MTR_8g023840 30.070 143 94 4 9 146 10 151 1.05e-13 68.6
MsG0780040000.01.T01 MTR_5g012080 29.688 128 86 3 10 133 7 134 1.49e-13 67.8
MsG0780040000.01.T01 MTR_4g134460 29.839 124 82 3 13 133 57 178 1.89e-13 67.8
MsG0780040000.01.T01 MTR_3g093050 33.071 127 81 3 10 133 22 147 2.11e-13 67.8
MsG0780040000.01.T01 MTR_3g093050 33.071 127 81 3 10 133 22 147 2.21e-13 67.8
MsG0780040000.01.T01 MTR_8g023900 29.518 166 109 6 10 168 12 176 4.54e-13 66.6
MsG0780040000.01.T01 MTR_1g090723 31.343 134 84 5 13 142 11 140 5.97e-13 66.2
MsG0780040000.01.T01 MTR_1g096430 29.323 133 89 4 5 133 2 133 7.26e-13 65.9
MsG0780040000.01.T01 MTR_8g063550 30.709 127 84 3 10 133 6 131 7.51e-13 65.9
MsG0780040000.01.T01 MTR_1g090723 30.370 135 84 5 13 142 11 140 7.62e-13 66.2
MsG0780040000.01.T01 MTR_8g063550 30.709 127 84 3 10 133 6 131 7.74e-13 65.9
MsG0780040000.01.T01 MTR_1g090723 30.370 135 84 5 13 142 11 140 7.85e-13 66.2
MsG0780040000.01.T01 MTR_8g023880 26.573 143 83 5 10 133 7 146 8.14e-13 65.1
MsG0780040000.01.T01 MTR_8g063550 30.709 127 84 3 10 133 6 131 8.42e-13 65.9
MsG0780040000.01.T01 MTR_2g086880 30.935 139 89 7 1 136 11 145 1.37e-12 65.5
MsG0780040000.01.T01 MTR_2g086880 30.935 139 89 7 1 136 11 145 1.38e-12 65.5
MsG0780040000.01.T01 MTR_3g093040 30.233 129 86 3 8 133 106 233 2.29e-12 64.3
MsG0780040000.01.T01 MTR_8g023860 29.452 146 89 5 10 146 12 152 4.75e-12 63.5
MsG0780040000.01.T01 MTR_2g086690 30.065 153 98 4 9 154 3 153 6.02e-12 62.4
MsG0780040000.01.T01 MTR_3g070040 31.387 137 79 4 3 133 1 128 9.80e-12 62.4
MsG0780040000.01.T01 MTR_8g023930 27.168 173 112 5 10 173 24 191 1.71e-11 62.0
MsG0780040000.01.T01 MTR_0036s0150 28.000 125 85 3 13 133 8 131 3.26e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040000.01.T01 AT5G13180 38.519 135 74 5 3 133 7 136 6.38e-24 94.7
MsG0780040000.01.T01 AT5G18270 37.209 129 76 4 8 133 19 145 1.13e-21 90.1
MsG0780040000.01.T01 AT5G18270 36.434 129 77 4 8 133 19 145 1.88e-21 89.7
MsG0780040000.01.T01 AT3G10500 35.036 137 84 4 10 142 9 144 6.02e-21 89.4
MsG0780040000.01.T01 AT1G77450 33.758 157 94 5 1 154 1 150 6.69e-21 86.7
MsG0780040000.01.T01 AT5G04410 35.433 127 78 3 10 133 9 134 7.08e-21 89.4
MsG0780040000.01.T01 AT5G63790 33.824 136 83 4 6 138 46 177 2.50e-20 86.3
MsG0780040000.01.T01 AT5G63790 33.824 136 83 4 6 138 56 187 2.61e-20 86.3
MsG0780040000.01.T01 AT5G08790 33.088 136 84 4 6 138 3 134 3.67e-20 85.5
MsG0780040000.01.T01 AT1G76420 36.641 131 77 5 7 133 19 147 4.69e-20 85.9
MsG0780040000.01.T01 AT2G24430 35.878 131 78 5 7 133 13 141 7.90e-20 85.1
MsG0780040000.01.T01 AT2G24430 35.878 131 78 5 7 133 13 141 7.90e-20 85.1
MsG0780040000.01.T01 AT3G04070 31.690 142 88 4 10 144 10 149 1.24e-19 84.7
MsG0780040000.01.T01 AT3G04070 31.690 142 88 4 10 144 10 149 1.77e-19 84.3
MsG0780040000.01.T01 AT1G61110 33.858 127 79 3 10 133 16 140 4.90e-19 82.8
MsG0780040000.01.T01 AT1G01720 32.836 134 83 4 8 138 5 134 2.02e-18 80.9
MsG0780040000.01.T01 AT5G53950 31.013 158 84 6 10 144 17 172 2.31e-18 81.6
MsG0780040000.01.T01 AT3G29035 34.615 130 79 5 7 133 21 147 2.46e-18 80.9
MsG0780040000.01.T01 AT5G61430 30.857 175 88 7 7 151 13 184 3.65e-18 80.5
MsG0780040000.01.T01 AT3G15500 31.206 141 88 5 6 143 10 144 3.88e-18 80.5
MsG0780040000.01.T01 AT3G10490 34.074 135 85 4 2 133 19 152 8.87e-18 78.2
MsG0780040000.01.T01 AT5G07680 33.333 132 82 5 5 133 12 140 1.01e-17 79.3
MsG0780040000.01.T01 AT1G52890 31.206 141 88 5 6 143 10 144 1.03e-17 79.3
MsG0780040000.01.T01 AT4G27410 31.298 131 84 4 6 133 10 137 1.11e-17 79.0
MsG0780040000.01.T01 AT5G07680 34.109 129 79 5 8 133 1 126 1.51e-17 78.6
MsG0780040000.01.T01 AT3G04060 33.333 129 81 4 8 133 18 144 2.01e-17 78.6
MsG0780040000.01.T01 AT2G33480 34.615 130 74 5 8 133 13 135 2.35e-17 77.4
MsG0780040000.01.T01 AT2G33480 34.615 130 74 5 8 133 13 135 2.39e-17 77.4
MsG0780040000.01.T01 AT5G39610 33.088 136 85 5 1 133 11 143 2.53e-17 77.8
MsG0780040000.01.T01 AT5G17260 32.824 131 84 3 6 133 2 131 2.62e-17 79.0
MsG0780040000.01.T01 AT3G10480 34.074 135 85 4 2 133 19 152 5.05e-17 78.2
MsG0780040000.01.T01 AT3G10480 34.074 135 85 4 2 133 19 152 5.16e-17 78.2
MsG0780040000.01.T01 AT3G15170 32.812 128 80 5 10 133 20 145 5.45e-17 77.0
MsG0780040000.01.T01 AT3G03200 33.588 131 83 3 6 133 2 131 6.05e-17 77.8
MsG0780040000.01.T01 AT3G10490 34.074 135 85 4 2 133 19 152 6.76e-17 77.8
MsG0780040000.01.T01 AT3G17730 33.588 131 83 3 6 133 2 131 7.86e-17 75.9
MsG0780040000.01.T01 AT3G15510 33.065 124 77 4 14 133 21 142 9.02e-17 77.0
MsG0780040000.01.T01 AT1G65910 32.061 131 85 3 6 133 2 131 9.04e-17 77.4
MsG0780040000.01.T01 AT3G18400 31.056 161 103 6 7 162 2 159 1.28e-16 76.3
MsG0780040000.01.T01 AT1G54330 32.824 131 84 3 6 133 2 131 2.44e-16 75.1
MsG0780040000.01.T01 AT1G69490 30.827 133 86 4 4 133 3 132 2.99e-16 74.7
MsG0780040000.01.T01 AT5G39820 29.545 132 87 4 13 140 23 152 5.98e-16 74.3
MsG0780040000.01.T01 AT1G34180 31.746 126 82 3 13 135 19 143 6.18e-16 75.1
MsG0780040000.01.T01 AT4G36160 30.657 137 90 4 1 133 5 140 1.08e-15 73.9
MsG0780040000.01.T01 AT1G34180 31.746 126 82 3 13 135 19 143 1.09e-15 74.3
MsG0780040000.01.T01 AT2G43000 33.846 130 79 5 7 133 16 141 1.13e-15 73.2
MsG0780040000.01.T01 AT3G12910 36.090 133 74 5 8 133 20 148 1.16e-15 73.6
MsG0780040000.01.T01 AT4G36160 30.657 137 90 4 1 133 1 136 1.23e-15 73.9
MsG0780040000.01.T01 AT4G36160 30.657 137 90 4 1 133 1 136 1.23e-15 73.9
MsG0780040000.01.T01 AT1G34190 31.387 137 85 4 13 141 19 154 1.28e-15 74.3
MsG0780040000.01.T01 AT1G26870 31.008 129 81 5 10 133 16 141 1.75e-15 73.6
MsG0780040000.01.T01 AT4G27410 28.767 146 85 5 6 133 10 154 3.52e-15 72.0
MsG0780040000.01.T01 AT5G66300 32.812 128 81 4 10 133 12 138 5.52e-15 71.2
MsG0780040000.01.T01 AT2G27300 34.307 137 85 5 1 135 7 140 9.75e-15 71.2
MsG0780040000.01.T01 AT4G17980 28.986 138 94 3 10 143 6 143 9.84e-15 70.5
MsG0780040000.01.T01 AT1G33060 33.858 127 80 4 10 133 24 149 1.15e-14 71.6
MsG0780040000.01.T01 AT1G33060 33.858 127 80 4 10 133 24 149 1.18e-14 71.2
MsG0780040000.01.T01 AT5G62380 29.771 131 81 5 10 133 7 133 1.83e-14 70.5
MsG0780040000.01.T01 AT5G62380 29.771 131 81 5 10 133 7 133 1.83e-14 70.5
MsG0780040000.01.T01 AT4G17980 28.986 138 94 3 10 143 6 143 2.27e-14 69.7
MsG0780040000.01.T01 AT1G79580 28.676 136 93 3 2 133 9 144 3.26e-14 69.7
MsG0780040000.01.T01 AT1G79580 28.676 136 93 3 2 133 9 144 3.26e-14 69.7
MsG0780040000.01.T01 AT1G79580 28.676 136 93 3 2 133 9 144 3.26e-14 69.7
MsG0780040000.01.T01 AT1G79580 28.676 136 93 3 2 133 9 144 3.26e-14 69.7
MsG0780040000.01.T01 AT1G79580 28.676 136 93 3 2 133 9 144 3.26e-14 69.7
MsG0780040000.01.T01 AT4G35580 33.071 127 81 3 10 133 9 134 5.30e-14 69.3
MsG0780040000.01.T01 AT5G46590 29.134 127 87 2 10 133 6 132 5.93e-14 68.6
MsG0780040000.01.T01 AT5G46590 29.134 127 87 2 10 133 6 132 6.10e-14 68.6
MsG0780040000.01.T01 AT1G34180 29.710 138 81 4 13 135 19 155 7.22e-14 68.9
MsG0780040000.01.T01 AT2G18060 31.250 128 83 4 10 133 9 135 7.39e-14 68.9
MsG0780040000.01.T01 AT2G18060 31.250 128 83 4 10 133 9 135 7.39e-14 68.9
MsG0780040000.01.T01 AT4G35580 33.071 127 81 3 10 133 9 134 7.50e-14 68.9
MsG0780040000.01.T01 AT4G35580 33.071 127 81 3 10 133 9 134 7.57e-14 68.9
MsG0780040000.01.T01 AT4G10350 32.353 136 87 4 10 141 9 143 1.08e-13 68.2
MsG0780040000.01.T01 AT1G34180 29.710 138 81 4 13 135 19 155 1.28e-13 68.2
MsG0780040000.01.T01 AT1G33280 32.308 130 83 4 10 135 8 136 1.43e-13 67.8
MsG0780040000.01.T01 AT3G12977 28.148 135 91 4 2 133 10 141 1.95e-13 67.0
MsG0780040000.01.T01 AT5G24590 31.496 127 84 2 10 133 13 139 3.16e-13 67.0
MsG0780040000.01.T01 AT5G14000 27.737 137 93 4 3 136 8 141 5.16e-13 63.9
MsG0780040000.01.T01 AT1G56010 29.412 136 89 4 2 133 11 143 5.52e-13 66.2
MsG0780040000.01.T01 AT2G02450 28.226 124 84 3 13 133 54 175 8.18e-13 65.9
MsG0780040000.01.T01 AT2G02450 28.226 124 84 3 13 133 54 175 1.41e-12 65.1
MsG0780040000.01.T01 AT1G71930 29.688 128 85 4 10 133 9 135 1.51e-12 64.7
MsG0780040000.01.T01 AT1G71930 29.688 128 85 4 10 133 9 135 1.51e-12 64.7
MsG0780040000.01.T01 AT5G64060 29.771 131 88 4 6 133 2 131 1.58e-12 65.1
MsG0780040000.01.T01 AT5G14000 27.737 137 93 4 3 136 8 141 3.34e-12 62.8
MsG0780040000.01.T01 AT5G04395 28.758 153 93 5 1 137 19 171 3.95e-12 62.0
MsG0780040000.01.T01 AT1G32870 30.709 127 86 2 10 135 10 135 4.84e-12 63.9
MsG0780040000.01.T01 AT1G32870 30.709 127 86 2 10 135 10 135 5.02e-12 63.9
MsG0780040000.01.T01 AT1G32870 30.709 127 86 2 10 135 46 171 5.17e-12 63.5
MsG0780040000.01.T01 AT1G02230 26.708 161 108 4 11 164 4 161 9.79e-12 62.8
MsG0780040000.01.T01 AT3G49530 28.346 127 88 2 10 133 13 139 1.82e-11 62.0
MsG0780040000.01.T01 AT3G49530 28.346 127 88 2 10 133 13 139 1.98e-11 62.0
MsG0780040000.01.T01 AT5G64530 29.231 130 78 5 8 135 1 118 6.25e-11 58.2
MsG0780040000.01.T01 AT3G44350 28.244 131 88 3 9 133 4 134 6.40e-11 58.2
MsG0780040000.01.T01 AT2G46770 25.676 148 104 5 4 146 10 156 8.71e-11 60.1

Find 47 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGATCTACCTGCTGGATTC+AGG 0.237662 7:-71805776 MsG0780040000.01.T01:CDS
GAACAGTCGGTTCGAAAATA+TGG 0.258410 7:-71805269 MsG0780040000.01.T01:CDS
GAAAGAGCACATTTGTTTAC+TGG 0.275802 7:-71805162 MsG0780040000.01.T01:CDS
TGCAGGTAATAGGTACTCTT+TGG 0.340271 7:-71805323 MsG0780040000.01.T01:intron
TCAAATTCAGACATGACATT+TGG 0.359524 7:+71805687 None:intergenic
ATTGTATTCCTTACCCTAGT+TGG 0.360335 7:+71805067 None:intergenic
AGTATTATTTCTTTGATCTC+AGG 0.394952 7:-71805291 MsG0780040000.01.T01:CDS
AATATGGACGCAAGACGAGC+TGG 0.462643 7:-71805253 MsG0780040000.01.T01:CDS
GACGCAAGACGAGCTGGAAA+TGG 0.464716 7:-71805247 MsG0780040000.01.T01:CDS
GCACATTTGTTTACTGGAGT+GGG 0.466777 7:-71805156 MsG0780040000.01.T01:CDS
TGAGTTCATGGAGAGGGAGG+AGG 0.476558 7:+71804798 None:intergenic
TCAGACATGACATTTGGAAA+AGG 0.488819 7:+71805693 None:intergenic
GAAGAAGGCCACCACACTAA+TGG 0.491907 7:-71804841 MsG0780040000.01.T01:CDS
ACTGAGCCATGGAGGCTGCC+AGG 0.492712 7:-71805654 MsG0780040000.01.T01:CDS
ACATGACATTTGGAAAAGGT+TGG 0.502196 7:+71805697 None:intergenic
AAACCTCCTGGCAGCCTCCA+TGG 0.506870 7:+71805648 None:intergenic
AGCACATTTGTTTACTGGAG+TGG 0.509000 7:-71805157 MsG0780040000.01.T01:CDS
TGTCAGCTGTTGGTGCCTAT+CGG 0.513489 7:-71804894 MsG0780040000.01.T01:intron
AAATTCATGCATCCACCATC+GGG 0.513722 7:+71805111 None:intergenic
TTTGTTTACTGGAGTGGGCA+AGG 0.516244 7:-71805151 MsG0780040000.01.T01:CDS
GAGTTCATGGAGAGGGAGGA+GGG 0.516664 7:+71804799 None:intergenic
CAAATTCATGCATCCACCAT+CGG 0.517794 7:+71805110 None:intergenic
GCTGAGCTTGATCTACCTGC+TGG 0.518168 7:-71805783 MsG0780040000.01.T01:CDS
GACGAGCTGGAAATGGTGAA+TGG 0.521079 7:-71805240 MsG0780040000.01.T01:CDS
ATCGGATCTTTAAGATATGA+AGG 0.521757 7:-71804876 MsG0780040000.01.T01:CDS
ATAGGTACTCTTTGGAGCAT+AGG 0.529909 7:-71805315 MsG0780040000.01.T01:CDS
AGGTGGCAAAACTGAGAAGA+AGG 0.540768 7:-71804856 MsG0780040000.01.T01:CDS
AGTTCATGGAGAGGGAGGAG+GGG 0.541559 7:+71804800 None:intergenic
ATATATTTCAAACTGAGCCA+TGG 0.550284 7:-71805665 MsG0780040000.01.T01:CDS
TGCATCCTCCATTAGTGTGG+TGG 0.554338 7:+71804833 None:intergenic
TGAAACATGAGTTCATGGAG+AGG 0.563520 7:+71804791 None:intergenic
GAGAGTAGTTGAAAAGAACA+AGG 0.579351 7:-71805218 MsG0780040000.01.T01:CDS
TCTTTGATCTCAGGAACAGT+CGG 0.584323 7:-71805282 MsG0780040000.01.T01:CDS
GGATCAAACCTGAATCCAGC+AGG 0.586690 7:+71805768 None:intergenic
ATCGAGCTCTGATGACCCGA+TGG 0.606095 7:-71805126 MsG0780040000.01.T01:CDS
ACATGAGTTCATGGAGAGGG+AGG 0.614894 7:+71804795 None:intergenic
GAAACATGAGTTCATGGAGA+GGG 0.632009 7:+71804792 None:intergenic
TCAACAAGTTGACGATCGGT+CGG 0.636753 7:+71805747 None:intergenic
GAAGGCCACCACACTAATGG+AGG 0.652157 7:-71804838 MsG0780040000.01.T01:CDS
TCATATCTTAAAGATCCGAT+AGG 0.652337 7:+71804879 None:intergenic
GAGCTCTGATGACCCGATGG+TGG 0.653753 7:-71805123 MsG0780040000.01.T01:CDS
GCACTCAACAAGTTGACGAT+CGG 0.655422 7:+71805743 None:intergenic
TATTTCAAACTGAGCCATGG+AGG 0.675314 7:-71805662 MsG0780040000.01.T01:CDS
ACTTGCATCCTCCATTAGTG+TGG 0.678198 7:+71804830 None:intergenic
GAGCCATGGAGGCTGCCAGG+AGG 0.693460 7:-71805651 MsG0780040000.01.T01:intron
TGGATGCATGAATTTGTCAT+CGG 0.708626 7:-71805103 MsG0780040000.01.T01:CDS
GGATCTTTAAGATATGAAGG+TGG 0.724453 7:-71804873 MsG0780040000.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATCAATATCAAAAAATTTG+AGG - Chr7:71805566-71805585 MsG0780040000.01.T01:intron 15.0%
!!! AAATTTTTTGATATTGATAG+TGG + Chr7:71805565-71805584 None:intergenic 15.0%
!!! ATATGATGTTTAATTTTATG+TGG - Chr7:71805190-71805209 MsG0780040000.01.T01:CDS 15.0%
!!! TATGATGTTTAATTTTATGT+GGG - Chr7:71805191-71805210 MsG0780040000.01.T01:CDS 15.0%
!! TCATAAAGAAAGAGAATAAA+GGG + Chr7:71805020-71805039 None:intergenic 20.0%
!! TTTATTGATAAAACACACAA+TGG + Chr7:71805043-71805062 None:intergenic 20.0%
!!! AATTTGTTTTTATATGTTGC+AGG - Chr7:71805261-71805280 MsG0780040000.01.T01:CDS 20.0%
!!! TTGTCTTGTTTATATTATTC+AGG - Chr7:71805639-71805658 MsG0780040000.01.T01:intron 20.0%
! AACATCATATAACCATTTCA+TGG + Chr7:71805180-71805199 None:intergenic 25.0%
! ACATCATATAACCATTTCAT+GGG + Chr7:71805179-71805198 None:intergenic 25.0%
! GTCATAAAGAAAGAGAATAA+AGG + Chr7:71805021-71805040 None:intergenic 25.0%
! TTGAACTCTCAAATTAATGT+AGG - Chr7:71805684-71805703 MsG0780040000.01.T01:CDS 25.0%
!! AGTATTATTTCTTTGATCTC+AGG - Chr7:71805310-71805329 MsG0780040000.01.T01:CDS 25.0%
!! ATTTTCTTAGTGTTCTTTGA+TGG - Chr7:71805069-71805088 MsG0780040000.01.T01:intron 25.0%
!! TAGAATTTTTGATCCAACTA+GGG - Chr7:71805521-71805540 MsG0780040000.01.T01:intron 25.0%
!! TTTTATATGTTGCAGGTAAT+AGG - Chr7:71805268-71805287 MsG0780040000.01.T01:CDS 25.0%
!!! TATTTTTATGTCCCATGAAA+TGG - Chr7:71805165-71805184 MsG0780040000.01.T01:CDS 25.0%
!!! TGCTAGTTTTATGTAAACTT+TGG - Chr7:71805609-71805628 MsG0780040000.01.T01:intron 25.0%
ATATATTTCAAACTGAGCCA+TGG - Chr7:71804936-71804955 MsG0780040000.01.T01:intron 30.0%
TCAAATTCAGACATGACATT+TGG + Chr7:71804917-71804936 None:intergenic 30.0%
TCATATCTTAAAGATCCGAT+AGG + Chr7:71805725-71805744 None:intergenic 30.0%
! GTAGAATTTTTGATCCAACT+AGG - Chr7:71805520-71805539 MsG0780040000.01.T01:intron 30.0%
!! ATCGGATCTTTAAGATATGA+AGG - Chr7:71805725-71805744 MsG0780040000.01.T01:CDS 30.0%
ACATGACATTTGGAAAAGGT+TGG + Chr7:71804907-71804926 None:intergenic 35.0%
ATTGTATTCCTTACCCTAGT+TGG + Chr7:71805537-71805556 None:intergenic 35.0%
GAGAGTAGTTGAAAAGAACA+AGG - Chr7:71805383-71805402 MsG0780040000.01.T01:intron 35.0%
TCAGACATGACATTTGGAAA+AGG + Chr7:71804911-71804930 None:intergenic 35.0%
TGGATGCATGAATTTGTCAT+CGG - Chr7:71805498-71805517 MsG0780040000.01.T01:intron 35.0%
TTGTGTCTTAGACATGCAAT+TGG - Chr7:71805118-71805137 MsG0780040000.01.T01:CDS 35.0%
! GAAAGAGCACATTTGTTTAC+TGG - Chr7:71805439-71805458 MsG0780040000.01.T01:intron 35.0%
! TTTTTGATCCAACTAGGGTA+AGG - Chr7:71805526-71805545 MsG0780040000.01.T01:intron 35.0%
!! GGATCTTTAAGATATGAAGG+TGG - Chr7:71805728-71805747 MsG0780040000.01.T01:CDS 35.0%
AAATTCATGCATCCACCATC+GGG + Chr7:71805493-71805512 None:intergenic 40.0%
AGCACATTTGTTTACTGGAG+TGG - Chr7:71805444-71805463 MsG0780040000.01.T01:intron 40.0%
CAAATTCATGCATCCACCAT+CGG + Chr7:71805494-71805513 None:intergenic 40.0%
GCACATTTGTTTACTGGAGT+GGG - Chr7:71805445-71805464 MsG0780040000.01.T01:intron 40.0%
GTCTTAGACATGCAATTGGA+GGG - Chr7:71805122-71805141 MsG0780040000.01.T01:CDS 40.0%
TATTTCAAACTGAGCCATGG+AGG - Chr7:71804939-71804958 MsG0780040000.01.T01:intron 40.0%
TCTTAGACATGCAATTGGAG+GGG - Chr7:71805123-71805142 MsG0780040000.01.T01:CDS 40.0%
TCTTTGATCTCAGGAACAGT+CGG - Chr7:71805319-71805338 MsG0780040000.01.T01:CDS 40.0%
TGCAGGTAATAGGTACTCTT+TGG - Chr7:71805278-71805297 MsG0780040000.01.T01:CDS 40.0%
TGTCTTAGACATGCAATTGG+AGG - Chr7:71805121-71805140 MsG0780040000.01.T01:CDS 40.0%
TTAATGTAGGTGTCAGCTGT+TGG - Chr7:71805697-71805716 MsG0780040000.01.T01:CDS 40.0%
! GTTTTCTTTCACAAACCTCC+TGG + Chr7:71804968-71804987 None:intergenic 40.0%
!! ATAGGTACTCTTTGGAGCAT+AGG - Chr7:71805286-71805305 MsG0780040000.01.T01:CDS 40.0%
!! GAACAGTCGGTTCGAAAATA+TGG - Chr7:71805332-71805351 MsG0780040000.01.T01:intron 40.0%
ACTTGCATCCTCCATTAGTG+TGG + Chr7:71805774-71805793 None:intergenic 45.0%
AGGTGGCAAAACTGAGAAGA+AGG - Chr7:71805745-71805764 MsG0780040000.01.T01:CDS 45.0%
TTGATCTACCTGCTGGATTC+AGG - Chr7:71804825-71804844 MsG0780040000.01.T01:CDS 45.0%
TTTGTTTACTGGAGTGGGCA+AGG - Chr7:71805450-71805469 MsG0780040000.01.T01:intron 45.0%
! GCACTCAACAAGTTGACGAT+CGG + Chr7:71804861-71804880 None:intergenic 45.0%
! GGTTGCAGATTTTCTTCGTC+AGG + Chr7:71804886-71804905 None:intergenic 45.0%
! TCAACAAGTTGACGATCGGT+CGG + Chr7:71804857-71804876 None:intergenic 45.0%
!!! AAAATTTAATTTATTCATTT+TGG - Chr7:71805230-71805249 MsG0780040000.01.T01:CDS 5.0%
!!! AAATTTAATTTATTCATTTT+GGG - Chr7:71805231-71805250 MsG0780040000.01.T01:CDS 5.0%
AATATGGACGCAAGACGAGC+TGG - Chr7:71805348-71805367 MsG0780040000.01.T01:intron 50.0%
GAAGAAGGCCACCACACTAA+TGG - Chr7:71805760-71805779 MsG0780040000.01.T01:CDS 50.0%
GACGAGCTGGAAATGGTGAA+TGG - Chr7:71805361-71805380 MsG0780040000.01.T01:intron 50.0%
GGATCAAACCTGAATCCAGC+AGG + Chr7:71804836-71804855 None:intergenic 50.0%
TAGACATGCAATTGGAGGGG+AGG - Chr7:71805126-71805145 MsG0780040000.01.T01:CDS 50.0%
TGCATCCTCCATTAGTGTGG+TGG + Chr7:71805771-71805790 None:intergenic 50.0%
! TGTCAGCTGTTGGTGCCTAT+CGG - Chr7:71805707-71805726 MsG0780040000.01.T01:CDS 50.0%
GAAGGCCACCACACTAATGG+AGG - Chr7:71805763-71805782 MsG0780040000.01.T01:CDS 55.0%
GACGCAAGACGAGCTGGAAA+TGG - Chr7:71805354-71805373 MsG0780040000.01.T01:intron 55.0%
GCTGAGCTTGATCTACCTGC+TGG - Chr7:71804818-71804837 MsG0780040000.01.T01:CDS 55.0%
! ATCGAGCTCTGATGACCCGA+TGG - Chr7:71805475-71805494 MsG0780040000.01.T01:intron 55.0%
AAACCTCCTGGCAGCCTCCA+TGG + Chr7:71804956-71804975 None:intergenic 60.0%
! GAGCTCTGATGACCCGATGG+TGG - Chr7:71805478-71805497 MsG0780040000.01.T01:intron 60.0%
ACTGAGCCATGGAGGCTGCC+AGG - Chr7:71804947-71804966 MsG0780040000.01.T01:intron 65.0%
GAGCCATGGAGGCTGCCAGG+AGG - Chr7:71804950-71804969 MsG0780040000.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr7 gene 71804803 71805820 71804803 ID=MsG0780040000.01;Name=MsG0780040000.01
Chr7 mRNA 71804803 71805820 71804803 ID=MsG0780040000.01.T01;Parent=MsG0780040000.01;Name=MsG0780040000.01.T01;_AED=0.21;_eAED=0.26;_QI=0|0|0|1|1|1|3|0|178
Chr7 exon 71805652 71805820 71805652 ID=MsG0780040000.01.T01:exon:14039;Parent=MsG0780040000.01.T01
Chr7 exon 71805081 71805340 71805081 ID=MsG0780040000.01.T01:exon:14038;Parent=MsG0780040000.01.T01
Chr7 exon 71804803 71804910 71804803 ID=MsG0780040000.01.T01:exon:14037;Parent=MsG0780040000.01.T01
Chr7 CDS 71805652 71805820 71805652 ID=MsG0780040000.01.T01:cds;Parent=MsG0780040000.01.T01
Chr7 CDS 71805081 71805340 71805081 ID=MsG0780040000.01.T01:cds;Parent=MsG0780040000.01.T01
Chr7 CDS 71804803 71804910 71804803 ID=MsG0780040000.01.T01:cds;Parent=MsG0780040000.01.T01
Gene Sequence

>MsG0780040000.01.T01

ATGGCTGTAGCTAATGCTGAGCTTGATCTACCTGCTGGATTCAGGTTTGATCCGACCGATCGTCAACTTGTTGAGTGCTACCTGACGAAGAAAATCTGCAACCAACCTTTTCCAAATGTCATGTCTGAATTTGATATATTTCAAACTGAGCCATGGAGGCTGCCAGGAGGTAATAGGTACTCTTTGGAGCATAGGAAGTATTATTTCTTTGATCTCAGGAACAGTCGGTTCGAAAATATGGACGCAAGACGAGCTGGAAATGGTGAATGGAGAGTAGTTGAAAAGAACAAGGAGTATGCTCATTCAAACAATCAATTGATTGTGAGAAAGAGCACATTTGTTTACTGGAGTGGGCAAGGAAATCGAGCTCTGATGACCCGATGGTGGATGCATGAATTTGTCATCGGTAGAATTTTTGATCCAACTAGGCTGTTGGTGCCTATCGGATCTTTAAGATATGAAGGTGGCAAAACTGAGAAGAAGGCCACCACACTAATGGAGGATGCAAGTGTTTCTGCCCCTCCTCCCTCTCCATGA
Protein sequence

>MsG0780040000.01.T01

MAVANAELDLPAGFRFDPTDRQLVECYLTKKICNQPFPNVMSEFDIFQTEPWRLPGGNRYSLEHRKYYFFDLRNSRFENMDARRAGNGEWRVVEKNKEYAHSNNQLIVRKSTFVYWSGQGNRALMTRWWMHEFVIGRIFDPTRLLVPIGSLRYEGGKTEKKATTLMEDASVSAPPPSP*