AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040004.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040004.01.T01 MTR_7g083360 71.613 155 43 1 1 155 1 154 8.39e-80 233
MsG0780040004.01.T01 MTR_7g083330 71.242 153 44 0 3 155 11 163 2.83e-77 228
MsG0780040004.01.T01 MTR_7g083370 77.778 135 30 0 1 135 1 135 3.21e-77 226
MsG0780040004.01.T01 MTR_2157s0010 31.469 143 91 4 3 140 1 141 4.42e-20 84.0
MsG0780040004.01.T01 MTR_7g085220 31.884 138 81 4 10 136 8 143 2.10e-19 83.2
MsG0780040004.01.T01 MTR_4g081870 29.412 153 100 5 4 153 3 150 4.49e-19 81.3
MsG0780040004.01.T01 MTR_2g064090 32.716 162 90 7 6 155 2 156 6.14e-19 82.0
MsG0780040004.01.T01 MTR_3g088110 33.588 131 80 4 6 133 3 129 6.76e-19 81.3
MsG0780040004.01.T01 MTR_5g041940 30.201 149 96 5 8 153 1 144 7.94e-19 80.5
MsG0780040004.01.T01 MTR_7g005280 32.394 142 87 5 5 142 10 146 8.75e-19 80.5
MsG0780040004.01.T01 MTR_4g089135 33.333 144 88 5 10 147 15 156 1.59e-18 80.9
MsG0780040004.01.T01 MTR_8g094580 33.333 129 79 4 8 133 5 129 4.46e-18 79.0
MsG0780040004.01.T01 MTR_7g100990 32.639 144 87 5 6 141 7 148 1.14e-17 78.2
MsG0780040004.01.T01 MTR_2g093810 30.714 140 91 4 4 139 8 145 1.20e-17 78.2
MsG0780040004.01.T01 MTR_5g090970 35.338 133 76 6 7 133 16 144 2.15e-17 77.4
MsG0780040004.01.T01 MTR_5g040420 32.847 137 85 5 7 139 12 145 2.54e-17 77.8
MsG0780040004.01.T01 MTR_6g011860 31.333 150 95 5 1 145 7 153 2.95e-17 77.8
MsG0780040004.01.T01 MTR_5g090970 35.338 133 75 6 7 133 16 143 4.37e-17 76.6
MsG0780040004.01.T01 MTR_2g080010 33.594 128 79 4 10 133 15 140 5.38e-17 76.6
MsG0780040004.01.T01 MTR_6g477900 34.646 127 79 3 10 133 6 131 5.42e-17 75.1
MsG0780040004.01.T01 MTR_3g070030 31.293 147 92 5 3 143 12 155 8.16e-17 76.3
MsG0780040004.01.T01 MTR_3g096920 32.836 134 77 5 6 133 3 129 1.50e-16 74.7
MsG0780040004.01.T01 MTR_2g068880 32.394 142 88 5 4 139 17 156 1.71e-16 75.1
MsG0780040004.01.T01 MTR_8g059170 29.221 154 93 6 6 153 10 153 1.73e-16 75.1
MsG0780040004.01.T01 MTR_2g068920 32.639 144 89 5 2 139 14 155 1.86e-16 74.7
MsG0780040004.01.T01 MTR_8g467490 32.847 137 85 5 7 139 12 145 1.92e-16 75.5
MsG0780040004.01.T01 MTR_3g096920 32.836 134 77 5 6 133 3 129 2.16e-16 74.7
MsG0780040004.01.T01 MTR_6g012670 31.884 138 88 4 4 138 14 148 2.36e-16 74.7
MsG0780040004.01.T01 MTR_6g032770 29.932 147 94 5 4 143 12 156 2.80e-16 74.7
MsG0780040004.01.T01 MTR_8g099750 29.496 139 90 4 3 137 7 141 3.07e-16 73.2
MsG0780040004.01.T01 MTR_5g076850 33.588 131 83 3 6 133 2 131 3.69e-16 74.7
MsG0780040004.01.T01 MTR_3g064580 32.824 131 84 3 6 133 2 131 4.29e-16 74.7
MsG0780040004.01.T01 MTR_1g008740 32.117 137 84 5 7 137 10 143 7.96e-16 73.6
MsG0780040004.01.T01 MTR_2g078700 31.250 128 82 5 10 133 23 148 8.17e-16 73.6
MsG0780040004.01.T01 MTR_1g008740 32.117 137 84 5 7 137 24 157 9.04e-16 73.2
MsG0780040004.01.T01 MTR_6g084430 28.859 149 100 5 10 155 5 150 1.35e-15 72.4
MsG0780040004.01.T01 MTR_2g079990 30.147 136 89 4 6 138 12 144 1.91e-15 72.4
MsG0780040004.01.T01 MTR_2g079990 30.147 136 89 4 6 138 12 144 2.04e-15 72.4
MsG0780040004.01.T01 MTR_1g096430 29.677 155 100 5 7 155 4 155 3.17e-15 71.6
MsG0780040004.01.T01 MTR_3g116070 30.657 137 86 5 10 141 23 155 3.43e-15 71.6
MsG0780040004.01.T01 MTR_3g109340 31.111 135 87 4 7 137 19 151 4.89e-15 71.2
MsG0780040004.01.T01 MTR_3g109340 31.111 135 87 4 7 137 19 151 4.96e-15 71.2
MsG0780040004.01.T01 MTR_3g435150 28.462 130 87 5 7 133 13 139 5.25e-15 70.9
MsG0780040004.01.T01 MTR_3g093050 33.071 127 81 3 10 133 22 147 7.14e-15 71.2
MsG0780040004.01.T01 MTR_1g090723 32.215 149 93 7 13 155 11 157 7.66e-15 70.9
MsG0780040004.01.T01 MTR_1g090723 32.215 149 93 7 13 155 11 157 7.95e-15 70.9
MsG0780040004.01.T01 MTR_3g093050 33.071 127 81 3 10 133 22 147 9.74e-15 70.9
MsG0780040004.01.T01 MTR_5g021710 30.519 154 100 5 7 155 5 156 9.84e-15 70.5
MsG0780040004.01.T01 MTR_7g085260 32.576 132 81 5 8 133 15 144 1.05e-14 70.5
MsG0780040004.01.T01 MTR_1g090723 33.333 129 79 6 13 136 11 137 1.34e-14 70.5
MsG0780040004.01.T01 MTR_4g036030 28.667 150 102 4 10 155 7 155 1.52e-14 69.7
MsG0780040004.01.T01 MTR_2g086880 31.884 138 87 7 1 135 11 144 3.00e-14 69.3
MsG0780040004.01.T01 MTR_1g090720 32.031 128 81 4 10 133 42 167 3.01e-14 69.3
MsG0780040004.01.T01 MTR_5g069030 33.858 127 80 3 10 133 25 150 3.32e-14 69.3
MsG0780040004.01.T01 MTR_2g086880 31.884 138 87 7 1 135 11 144 3.38e-14 69.3
MsG0780040004.01.T01 MTR_1g069805 31.333 150 94 6 1 145 1 146 3.60e-14 68.2
MsG0780040004.01.T01 MTR_3g098810 29.078 141 90 4 3 137 7 143 5.34e-14 66.2
MsG0780040004.01.T01 MTR_1g097300 29.054 148 99 4 13 155 22 168 5.59e-14 68.6
MsG0780040004.01.T01 MTR_4g108760 28.462 130 87 5 7 133 14 140 5.65e-14 68.2
MsG0780040004.01.T01 MTR_3g093040 31.008 129 85 3 8 133 106 233 1.25e-13 67.0
MsG0780040004.01.T01 MTR_3g098810 29.078 141 90 4 3 137 7 143 1.55e-13 65.9
MsG0780040004.01.T01 MTR_8g023880 28.671 143 80 4 10 133 7 146 1.88e-13 66.2
MsG0780040004.01.T01 MTR_8g076110 28.105 153 105 4 7 155 3 154 2.21e-13 66.2
MsG0780040004.01.T01 MTR_7g097090 31.126 151 93 6 1 145 1 146 4.25e-13 65.5
MsG0780040004.01.T01 MTR_5g012080 28.906 128 87 3 10 133 7 134 4.37e-13 65.9
MsG0780040004.01.T01 MTR_0036s0150 30.882 136 73 6 13 136 8 134 4.42e-13 65.9
MsG0780040004.01.T01 MTR_5g014300 28.387 155 101 4 10 155 6 159 4.58e-13 65.5
MsG0780040004.01.T01 MTR_4g101680 27.815 151 102 5 10 155 7 155 5.77e-13 65.5
MsG0780040004.01.T01 MTR_2g064470 29.787 141 81 6 3 135 4 134 7.98e-13 64.7
MsG0780040004.01.T01 MTR_2g062730 27.044 159 112 3 1 155 1 159 9.31e-13 64.7
MsG0780040004.01.T01 MTR_8g023900 28.966 145 97 4 9 148 11 154 1.15e-12 64.7
MsG0780040004.01.T01 MTR_7g011120 29.545 132 87 4 5 133 3 131 1.76e-12 63.9
MsG0780040004.01.T01 MTR_4g134460 26.316 152 106 4 7 155 52 200 2.58e-12 63.5
MsG0780040004.01.T01 MTR_7g011130 28.358 134 90 4 3 133 12 142 1.12e-11 61.6
MsG0780040004.01.T01 MTR_4g098630 25.490 153 107 4 10 155 6 158 1.41e-11 61.2
MsG0780040004.01.T01 MTR_8g023840 31.008 129 83 5 10 133 11 138 3.86e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040004.01.T01 AT5G13180 37.037 135 76 5 3 133 7 136 1.31e-20 85.1
MsG0780040004.01.T01 AT2G33480 33.582 134 80 4 8 138 13 140 7.10e-20 83.6
MsG0780040004.01.T01 AT2G33480 33.582 134 80 4 8 138 13 140 7.17e-20 83.6
MsG0780040004.01.T01 AT2G43000 35.897 156 88 8 7 155 16 166 1.74e-19 82.4
MsG0780040004.01.T01 AT3G03200 33.333 162 89 5 6 155 2 156 8.07e-19 82.4
MsG0780040004.01.T01 AT1G61110 34.646 127 78 4 10 133 16 140 1.31e-18 80.9
MsG0780040004.01.T01 AT1G77450 33.088 136 84 4 1 133 1 132 2.21e-18 79.3
MsG0780040004.01.T01 AT5G39820 32.168 143 88 6 7 143 18 157 2.75e-18 80.1
MsG0780040004.01.T01 AT5G63790 32.824 131 81 4 6 133 56 182 5.01e-18 79.3
MsG0780040004.01.T01 AT5G63790 32.824 131 81 4 6 133 46 172 5.15e-18 79.0
MsG0780040004.01.T01 AT1G76420 33.594 128 79 4 10 133 22 147 5.60e-18 79.3
MsG0780040004.01.T01 AT3G10500 33.846 130 82 4 10 136 9 137 7.42e-18 79.7
MsG0780040004.01.T01 AT1G65910 32.824 131 84 3 6 133 2 131 8.84e-18 79.7
MsG0780040004.01.T01 AT5G08790 32.061 131 82 4 6 133 3 129 9.97e-18 78.2
MsG0780040004.01.T01 AT1G69490 32.090 134 83 5 4 133 3 132 1.65e-17 77.0
MsG0780040004.01.T01 AT3G17730 34.615 130 83 2 6 133 2 131 2.52e-17 76.3
MsG0780040004.01.T01 AT5G18270 33.071 127 80 4 10 133 21 145 2.75e-17 77.4
MsG0780040004.01.T01 AT1G26870 34.091 132 78 6 7 133 14 141 3.73e-17 77.4
MsG0780040004.01.T01 AT3G04070 29.927 137 87 4 10 139 10 144 4.85e-17 76.6
MsG0780040004.01.T01 AT5G17260 31.481 162 92 5 6 155 2 156 5.67e-17 77.0
MsG0780040004.01.T01 AT3G04070 28.767 146 95 4 1 139 1 144 7.14e-17 76.3
MsG0780040004.01.T01 AT3G15170 31.298 131 84 5 7 133 17 145 1.00e-16 75.5
MsG0780040004.01.T01 AT2G24430 29.221 154 102 5 7 155 13 164 1.21e-16 75.5
MsG0780040004.01.T01 AT2G24430 29.221 154 102 5 7 155 13 164 1.21e-16 75.5
MsG0780040004.01.T01 AT5G04410 30.709 127 84 3 10 133 9 134 1.27e-16 76.3
MsG0780040004.01.T01 AT5G53950 32.812 128 80 5 10 133 17 142 1.66e-16 75.5
MsG0780040004.01.T01 AT5G18270 32.283 127 81 4 10 133 21 145 1.67e-16 75.1
MsG0780040004.01.T01 AT3G12910 35.338 133 75 5 8 133 20 148 2.80e-16 74.3
MsG0780040004.01.T01 AT3G15500 32.836 134 84 4 6 136 10 140 3.49e-16 74.3
MsG0780040004.01.T01 AT3G04060 30.709 127 83 4 10 133 20 144 4.12e-16 74.3
MsG0780040004.01.T01 AT1G01720 32.576 132 81 5 6 133 2 129 4.48e-16 73.6
MsG0780040004.01.T01 AT3G15510 32.283 127 80 4 14 136 21 145 5.02e-16 73.9
MsG0780040004.01.T01 AT1G33060 33.858 127 80 4 10 133 24 149 6.71e-16 74.3
MsG0780040004.01.T01 AT1G33060 33.858 127 80 4 10 133 24 149 7.45e-16 73.9
MsG0780040004.01.T01 AT1G52890 31.034 145 90 5 6 147 10 147 8.45e-16 73.2
MsG0780040004.01.T01 AT3G10490 30.935 139 90 4 2 136 19 155 9.60e-16 72.0
MsG0780040004.01.T01 AT5G07680 30.303 132 86 4 5 133 12 140 1.32e-15 72.8
MsG0780040004.01.T01 AT3G29035 30.065 153 95 6 10 154 24 172 1.41e-15 72.4
MsG0780040004.01.T01 AT4G27410 30.597 134 87 4 6 136 10 140 1.64e-15 72.0
MsG0780040004.01.T01 AT5G07680 31.496 127 81 4 10 133 3 126 1.70e-15 72.4
MsG0780040004.01.T01 AT1G54330 32.075 159 91 5 6 155 2 152 1.74e-15 72.0
MsG0780040004.01.T01 AT5G39610 32.283 127 80 5 10 133 20 143 1.92e-15 71.6
MsG0780040004.01.T01 AT2G27300 32.278 158 95 8 1 155 7 155 2.74e-15 72.0
MsG0780040004.01.T01 AT5G62380 29.801 151 99 5 10 155 7 155 3.44e-15 71.6
MsG0780040004.01.T01 AT5G62380 29.801 151 99 5 10 155 7 155 3.44e-15 71.6
MsG0780040004.01.T01 AT4G36160 29.375 160 106 5 1 155 1 158 4.07e-15 71.6
MsG0780040004.01.T01 AT4G36160 29.375 160 106 5 1 155 1 158 4.07e-15 71.6
MsG0780040004.01.T01 AT4G36160 29.375 160 106 5 1 155 5 162 4.28e-15 71.6
MsG0780040004.01.T01 AT4G35580 34.646 127 79 4 10 133 9 134 4.80e-15 71.6
MsG0780040004.01.T01 AT4G35580 34.646 127 79 4 10 133 9 134 5.13e-15 71.6
MsG0780040004.01.T01 AT4G35580 34.646 127 79 4 10 133 9 134 5.28e-15 71.6
MsG0780040004.01.T01 AT5G46590 26.000 150 108 2 9 155 5 154 5.46e-15 70.5
MsG0780040004.01.T01 AT3G18400 30.534 131 85 5 10 137 5 132 5.50e-15 70.9
MsG0780040004.01.T01 AT5G61430 30.769 130 84 5 7 133 13 139 6.95e-15 70.9
MsG0780040004.01.T01 AT3G10490 30.935 139 90 4 2 136 19 155 1.00e-14 70.5
MsG0780040004.01.T01 AT3G10480 30.216 139 91 4 2 136 19 155 1.46e-14 70.1
MsG0780040004.01.T01 AT3G10480 30.216 139 91 4 2 136 19 155 1.52e-14 70.1
MsG0780040004.01.T01 AT3G44290 32.283 127 81 4 13 136 17 141 1.59e-14 68.6
MsG0780040004.01.T01 AT1G71930 28.667 150 102 4 10 155 9 157 1.83e-14 69.3
MsG0780040004.01.T01 AT1G71930 28.667 150 102 4 10 155 9 157 1.83e-14 69.3
MsG0780040004.01.T01 AT5G46590 28.906 128 88 2 9 133 5 132 3.52e-14 68.2
MsG0780040004.01.T01 AT1G34180 32.308 130 72 4 13 133 19 141 4.46e-14 68.9
MsG0780040004.01.T01 AT3G44290 32.283 127 81 4 13 136 17 141 5.17e-14 68.2
MsG0780040004.01.T01 AT3G12977 28.472 144 95 5 2 141 10 149 5.79e-14 67.8
MsG0780040004.01.T01 AT4G17980 27.097 155 105 4 9 155 5 159 6.70e-14 67.4
MsG0780040004.01.T01 AT1G56010 29.655 145 94 5 2 142 11 151 8.03e-14 67.8
MsG0780040004.01.T01 AT4G17980 26.950 141 99 3 9 145 5 145 1.07e-13 67.0
MsG0780040004.01.T01 AT5G22290 30.657 137 89 5 2 135 14 147 1.15e-13 67.4
MsG0780040004.01.T01 AT2G18060 29.801 151 99 5 10 155 9 157 1.35e-13 67.4
MsG0780040004.01.T01 AT2G18060 29.801 151 99 5 10 155 9 157 1.35e-13 67.4
MsG0780040004.01.T01 AT1G79580 27.941 136 94 3 2 133 9 144 1.45e-13 67.0
MsG0780040004.01.T01 AT1G79580 27.941 136 94 3 2 133 9 144 1.45e-13 67.0
MsG0780040004.01.T01 AT1G79580 27.941 136 94 3 2 133 9 144 1.45e-13 67.0
MsG0780040004.01.T01 AT1G79580 27.941 136 94 3 2 133 9 144 1.45e-13 67.0
MsG0780040004.01.T01 AT1G79580 27.941 136 94 3 2 133 9 144 1.45e-13 67.0
MsG0780040004.01.T01 AT5G66300 29.801 151 100 5 10 155 12 161 1.59e-13 66.6
MsG0780040004.01.T01 AT1G34190 30.769 130 74 4 13 133 19 141 2.20e-13 67.0
MsG0780040004.01.T01 AT1G34180 31.818 132 74 4 13 135 19 143 3.47e-13 66.2
MsG0780040004.01.T01 AT1G12260 27.333 150 104 4 10 155 7 155 3.93e-13 65.9
MsG0780040004.01.T01 AT5G24590 31.496 127 84 2 10 133 13 139 5.11e-13 65.9
MsG0780040004.01.T01 AT1G12260 27.333 150 104 4 10 155 7 155 6.25e-13 65.5
MsG0780040004.01.T01 AT5G14000 28.058 139 88 6 3 135 8 140 1.11e-12 62.4
MsG0780040004.01.T01 AT5G50820 28.395 162 101 4 2 155 9 163 1.21e-12 62.8
MsG0780040004.01.T01 AT5G04395 29.605 152 89 6 1 135 19 169 1.88e-12 62.4
MsG0780040004.01.T01 AT2G02450 25.828 151 106 4 8 155 50 197 1.98e-12 63.9
MsG0780040004.01.T01 AT2G02450 25.828 151 106 4 8 155 50 197 2.41e-12 63.9
MsG0780040004.01.T01 AT5G09330 33.858 127 80 4 10 133 6 131 3.36e-12 63.5
MsG0780040004.01.T01 AT5G09330 33.858 127 80 4 10 133 6 131 3.36e-12 63.5
MsG0780040004.01.T01 AT5G09330 33.858 127 80 4 10 133 6 131 3.36e-12 63.5
MsG0780040004.01.T01 AT5G09330 33.858 127 80 4 10 133 6 131 3.36e-12 63.5
MsG0780040004.01.T01 AT5G09330 33.858 127 80 4 10 133 6 131 3.36e-12 63.5
MsG0780040004.01.T01 AT5G14000 28.058 139 88 6 3 135 8 140 4.20e-12 61.6
MsG0780040004.01.T01 AT4G28530 28.758 153 87 4 1 133 1 151 5.90e-12 62.4
MsG0780040004.01.T01 AT3G49530 29.134 127 87 2 10 133 13 139 1.72e-11 61.2
MsG0780040004.01.T01 AT3G44350 29.630 135 89 3 10 138 5 139 2.84e-11 58.5
MsG0780040004.01.T01 AT1G32870 31.200 125 84 2 10 133 10 133 4.07e-11 60.5
MsG0780040004.01.T01 AT1G32870 31.200 125 84 2 10 133 10 133 4.08e-11 60.5
MsG0780040004.01.T01 AT1G32870 31.200 125 84 2 10 133 46 169 4.22e-11 60.5
MsG0780040004.01.T01 AT1G32510 28.358 134 87 3 9 133 5 138 7.37e-11 59.3
MsG0780040004.01.T01 AT1G52880 29.365 126 81 4 14 133 21 144 8.18e-11 59.3

Find 36 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAGAACACATTTGTTTAC+TGG 0.271032 7:-71837479 MsG0780040004.01.T01:CDS
AGTACTATTTCTTTGATATC+AGG 0.279804 7:-71837608 MsG0780040004.01.T01:CDS
AGGTGCCTATCGGATATTTA+AGG 0.283056 7:-71837163 None:intergenic
CTTTCACACAATCTATTGAT+TGG 0.327245 7:-71837504 MsG0780040004.01.T01:CDS
GAACCGTAGATTCGAGAATA+TGG 0.360249 7:-71837586 MsG0780040004.01.T01:CDS
TTTCACACAATCTATTGATT+GGG 0.415597 7:-71837503 MsG0780040004.01.T01:CDS
ATTGTATTCATTACCCTAGC+TGG 0.431951 7:+71837381 None:intergenic
ACACATTTGTTTACTGGATA+GGG 0.438722 7:-71837473 MsG0780040004.01.T01:CDS
GGATCAAACTTGTATCCAAC+AGG 0.462546 7:+71838126 None:intergenic
GCTGAGCTTCTTCTACCTGT+TGG 0.469612 7:-71838141 MsG0780040004.01.T01:CDS
AACACATTTGTTTACTGGAT+AGG 0.480417 7:-71837474 MsG0780040004.01.T01:CDS
AAACCTCTTGGCAGCCTCCA+TGG 0.486538 7:+71838006 None:intergenic
GAAGAGCTGGAGATGGTGAA+TGG 0.497231 7:-71837557 MsG0780040004.01.T01:CDS
CATCCATATTCTCGAATCTA+CGG 0.497709 7:+71837583 None:intergenic
TCGAATTCAAACAAGTGAGT+TGG 0.514689 7:+71838045 None:intergenic
AATATGGATGCAAGAAGAGC+TGG 0.523452 7:-71837570 MsG0780040004.01.T01:CDS
CTTGTTGAGTACTACCTGAC+GGG 0.535841 7:-71838090 MsG0780040004.01.T01:CDS
TTTGTTTACTGGATAGGGCA+AGG 0.546149 7:-71837468 MsG0780040004.01.T01:CDS
GTGAATGGAGAATAGTTGAA+TGG 0.546179 7:-71837542 MsG0780040004.01.T01:CDS
GTACAATTCATCATCCAGCT+AGG 0.552549 7:-71837395 MsG0780040004.01.T01:CDS
TCAACAAGCTCGTGATCAGT+TGG 0.555838 7:+71838105 None:intergenic
TCAAACAAGTGAGTTGGAAG+AGG 0.558737 7:+71838051 None:intergenic
GAGAATAGTTGAATGGAATG+AGG 0.563671 7:-71837535 MsG0780040004.01.T01:CDS
ATGTGTTTCAAACTGAGCCA+TGG 0.569635 7:-71838023 MsG0780040004.01.T01:CDS
TACAATTCATCATCCAGCTA+GGG 0.576583 7:-71837394 MsG0780040004.01.T01:intron
AGACAGCTGTAGGTGCCTAT+CGG 0.582547 7:-71837173 MsG0780040004.01.T01:CDS
GCTTGTTGAGTACTACCTGA+CGG 0.620029 7:-71838091 MsG0780040004.01.T01:CDS
GATGCAAGAAGAGCTGGAGA+TGG 0.639988 7:-71837564 MsG0780040004.01.T01:CDS
TCATACCTTAAATATCCGAT+AGG 0.640099 7:+71837158 None:intergenic
TCAATGTAGGAGACAGCTGT+AGG 0.645111 7:-71837183 MsG0780040004.01.T01:intron
ACAAGTGAGTTGGAAGAGGT+AGG 0.647632 7:+71838055 None:intergenic
ATCAAGCTCTGATGACACAA+TGG 0.656016 7:-71837443 MsG0780040004.01.T01:CDS
GAGCCATGGAGGCTGCCAAG+AGG 0.670912 7:-71838009 MsG0780040004.01.T01:intron
TTGTTGAGTACTACCTGACG+GGG 0.672691 7:-71838089 MsG0780040004.01.T01:CDS
AAGCTCTGATGACACAATGG+TGG 0.675163 7:-71837440 MsG0780040004.01.T01:CDS
TGTTTCAAACTGAGCCATGG+AGG 0.716472 7:-71838020 MsG0780040004.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACAATTTGATTTATTCATTT+TGG - Chr7:71837631-71837650 MsG0780040004.01.T01:CDS 15.0%
!!! CAATTTGATTTATTCATTTT+GGG - Chr7:71837632-71837651 MsG0780040004.01.T01:CDS 15.0%
!!! TATCTTGTTTATTTATAATC+AGG - Chr7:71838081-71838100 MsG0780040004.01.T01:CDS 15.0%
!!! TTTTTTTAAGTAATGTGTTT+TGG - Chr7:71838000-71838019 MsG0780040004.01.T01:intron 15.0%
!! AACATCATATAAACATTTCA+TGG + Chr7:71837593-71837612 None:intergenic 20.0%
!! ACATCATATAAACATTTCAT+GGG + Chr7:71837592-71837611 None:intergenic 20.0%
!! CCATCAAAAGATTAATTAAA+TGG + Chr7:71837484-71837503 None:intergenic 20.0%
!!! CCATTTAATTAATCTTTTGA+TGG - Chr7:71837481-71837500 MsG0780040004.01.T01:CDS 20.0%
!!! TTTATATGATGTTTTCATGT+GGG - Chr7:71837600-71837619 MsG0780040004.01.T01:CDS 20.0%
!!! TTTTTTTTTTTGCAGATGAT+AGG - Chr7:71837670-71837689 MsG0780040004.01.T01:intron 20.0%
! TTTCTATTGATAAGACACAA+TGG + Chr7:71837408-71837427 None:intergenic 25.0%
!! AGTACTATTTCTTTGATATC+AGG - Chr7:71837712-71837731 MsG0780040004.01.T01:intron 25.0%
!! GTTTATATGATGTTTTCATG+TGG - Chr7:71837599-71837618 MsG0780040004.01.T01:CDS 25.0%
!! TCACTATCAAAAAACTTTTG+AGG - Chr7:71837973-71837992 MsG0780040004.01.T01:intron 25.0%
!! TTTCACACAATCTATTGATT+GGG - Chr7:71837817-71837836 MsG0780040004.01.T01:intron 25.0%
!! TTTTGAACTCAAATCAATGT+AGG - Chr7:71838124-71838143 MsG0780040004.01.T01:CDS 25.0%
!!! AAGTTTTTTGATAGTGATAG+TGG + Chr7:71837970-71837989 None:intergenic 25.0%
!!! TGCTAGTTTTTATGTAAACT+TGG - Chr7:71838050-71838069 MsG0780040004.01.T01:CDS 25.0%
AACACATTTGTTTACTGGAT+AGG - Chr7:71837846-71837865 MsG0780040004.01.T01:intron 30.0%
ACACATTTGTTTACTGGATA+GGG - Chr7:71837847-71837866 MsG0780040004.01.T01:intron 30.0%
CTTTCACACAATCTATTGAT+TGG - Chr7:71837816-71837835 MsG0780040004.01.T01:intron 30.0%
GAAAGAACACATTTGTTTAC+TGG - Chr7:71837841-71837860 MsG0780040004.01.T01:intron 30.0%
!! AGTAATGTGTTTTGGACTTA+TGG - Chr7:71838008-71838027 MsG0780040004.01.T01:intron 30.0%
ATTGTATTCATTACCCTAGC+TGG + Chr7:71837942-71837961 None:intergenic 35.0%
CATCCATATTCTCGAATCTA+CGG + Chr7:71837740-71837759 None:intergenic 35.0%
CTGAAATTTCACAAACCTCT+TGG + Chr7:71837329-71837348 None:intergenic 35.0%
GAGAATAGTTGAATGGAATG+AGG - Chr7:71837785-71837804 MsG0780040004.01.T01:intron 35.0%
GTGAATGGAGAATAGTTGAA+TGG - Chr7:71837778-71837797 MsG0780040004.01.T01:intron 35.0%
TACAATTCATCATCCAGCTA+GGG - Chr7:71837926-71837945 MsG0780040004.01.T01:intron 35.0%
TCGAATTCAAACAAGTGAGT+TGG + Chr7:71837278-71837297 None:intergenic 35.0%
! TGCAGATGATAGGTTTTCTT+TGG - Chr7:71837680-71837699 MsG0780040004.01.T01:intron 35.0%
!!! ATAGGTTTTCTTTGGAGCAT+AGG - Chr7:71837688-71837707 MsG0780040004.01.T01:intron 35.0%
AATATGGATGCAAGAAGAGC+TGG - Chr7:71837750-71837769 MsG0780040004.01.T01:intron 40.0%
ATGTGTTTCAAACTGAGCCA+TGG - Chr7:71837297-71837316 MsG0780040004.01.T01:intron 40.0%
GAACCGTAGATTCGAGAATA+TGG - Chr7:71837734-71837753 MsG0780040004.01.T01:intron 40.0%
GGATCAAACTTGTATCCAAC+AGG + Chr7:71837197-71837216 None:intergenic 40.0%
GTACAATTCATCATCCAGCT+AGG - Chr7:71837925-71837944 MsG0780040004.01.T01:intron 40.0%
TCTTAGACATGCAGTTGGAA+GGG - Chr7:71837534-71837553 MsG0780040004.01.T01:CDS 40.0%
TTTGTTTACTGGATAGGGCA+AGG - Chr7:71837852-71837871 MsG0780040004.01.T01:intron 40.0%
! ATCAAGCTCTGATGACACAA+TGG - Chr7:71837877-71837896 MsG0780040004.01.T01:intron 40.0%
! TCAAACAAGTGAGTTGGAAG+AGG + Chr7:71837272-71837291 None:intergenic 40.0%
CTTGTTGAGTACTACCTGAC+GGG - Chr7:71837230-71837249 MsG0780040004.01.T01:intron 45.0%
GTCTTAGACATGCAGTTGGA+AGG - Chr7:71837533-71837552 MsG0780040004.01.T01:CDS 45.0%
GTGTGTCTTAGACATGCAGT+TGG - Chr7:71837529-71837548 MsG0780040004.01.T01:CDS 45.0%
TCAACAAGCTCGTGATCAGT+TGG + Chr7:71837218-71837237 None:intergenic 45.0%
TCAATGTAGGAGACAGCTGT+AGG - Chr7:71838137-71838156 MsG0780040004.01.T01:CDS 45.0%
TGTTTCAAACTGAGCCATGG+AGG - Chr7:71837300-71837319 MsG0780040004.01.T01:intron 45.0%
TTGTTGAGTACTACCTGACG+GGG - Chr7:71837231-71837250 MsG0780040004.01.T01:intron 45.0%
! AAGCTCTGATGACACAATGG+TGG - Chr7:71837880-71837899 MsG0780040004.01.T01:intron 45.0%
! ACAAGTGAGTTGGAAGAGGT+AGG + Chr7:71837268-71837287 None:intergenic 45.0%
! GCTTGTTGAGTACTACCTGA+CGG - Chr7:71837229-71837248 MsG0780040004.01.T01:intron 45.0%
!!! ACAAAAATTTAAAATTTAAA+TGG + Chr7:71837448-71837467 None:intergenic 5.0%
AGACAGCTGTAGGTGCCTAT+CGG - Chr7:71838147-71838166 MsG0780040004.01.T01:CDS 50.0%
GAAGAGCTGGAGATGGTGAA+TGG - Chr7:71837763-71837782 MsG0780040004.01.T01:intron 50.0%
GATGCAAGAAGAGCTGGAGA+TGG - Chr7:71837756-71837775 MsG0780040004.01.T01:intron 50.0%
GCTGAGCTTCTTCTACCTGT+TGG - Chr7:71837179-71837198 MsG0780040004.01.T01:intron 50.0%
TAGACATGCAGTTGGAAGGG+AGG - Chr7:71837537-71837556 MsG0780040004.01.T01:CDS 50.0%
! GGTTGAGAATTTTCCCCGTC+AGG + Chr7:71837247-71837266 None:intergenic 50.0%
AAACCTCTTGGCAGCCTCCA+TGG + Chr7:71837317-71837336 None:intergenic 55.0%
GAGCCATGGAGGCTGCCAAG+AGG - Chr7:71837311-71837330 MsG0780040004.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr7 gene 71837164 71838178 71837164 ID=MsG0780040004.01;Name=MsG0780040004.01
Chr7 mRNA 71837164 71838178 71837164 ID=MsG0780040004.01.T01;Parent=MsG0780040004.01;Name=MsG0780040004.01.T01;_AED=0.06;_eAED=0.06;_QI=0|0|0|1|0|0|3|0|155
Chr7 exon 71838010 71838178 71838010 ID=MsG0780040004.01.T01:exon:14049;Parent=MsG0780040004.01.T01
Chr7 exon 71837395 71837657 71837395 ID=MsG0780040004.01.T01:exon:14048;Parent=MsG0780040004.01.T01
Chr7 exon 71837164 71837196 71837164 ID=MsG0780040004.01.T01:exon:14047;Parent=MsG0780040004.01.T01
Chr7 CDS 71838010 71838178 71838010 ID=MsG0780040004.01.T01:cds;Parent=MsG0780040004.01.T01
Chr7 CDS 71837395 71837657 71837395 ID=MsG0780040004.01.T01:cds;Parent=MsG0780040004.01.T01
Chr7 CDS 71837164 71837196 71837164 ID=MsG0780040004.01.T01:cds;Parent=MsG0780040004.01.T01
Gene Sequence

>MsG0780040004.01.T01

ATGGCTGTAGCTAATGCTGAGCTTCTTCTACCTGTTGGATACAAGTTTGATCCAACTGATCACGAGCTTGTTGAGTACTACCTGACGGGGAAAATTCTCAACCTACCTCTTCCAACTCACTTGTTTGAATTCGATGTGTTTCAAACTGAGCCATGGAGGCTGCCAAGAGATGATAGGTTTTCTTTGGAGCATAGGAAGTACTATTTCTTTGATATCAGGAACCGTAGATTCGAGAATATGGATGCAAGAAGAGCTGGAGATGGTGAATGGAGAATAGTTGAATGGAATGAGGATTTTGCTCTTTCACACAATCTATTGATTGGGAGAAAGAACACATTTGTTTACTGGATAGGGCAAGGAAATCAAGCTCTGATGACACAATGGTGGATGCATGAATTCTTCATCATCAGTACAATTCATCATCCAGCTAGGGAGACAGCTGTAGGTGCCTATCGGATATTTAAG
Protein sequence

>MsG0780040004.01.T01

MAVANAELLLPVGYKFDPTDHELVEYYLTGKILNLPLPTHLFEFDVFQTEPWRLPRDDRFSLEHRKYYFFDIRNRRFENMDARRAGDGEWRIVEWNEDFALSHNLLIGRKNTFVYWIGQGNQALMTQWWMHEFFIISTIHHPARETAVGAYRIFK