Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040468.01.T01 | AES81155.1 | 100 | 88 | 0 | 0 | 1 | 88 | 1 | 88 | 1.88E-57 | 181 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040468.01.T01 | Q58FS3 | 71.111 | 90 | 23 | 2 | 1 | 88 | 1 | 89 | 1.99E-39 | 128 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040468.01.T01 | G7L5W0 | 100.000 | 88 | 0 | 0 | 1 | 88 | 1 | 88 | 8.96e-58 | 181 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0780040468.01.T01 | TF | MYB-related |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040468.01.T01 | MTR_7g089210 | 100.000 | 88 | 0 | 0 | 1 | 88 | 1 | 88 | 2.27e-61 | 181 |
| MsG0780040468.01.T01 | MTR_3g013440 | 73.256 | 86 | 22 | 1 | 4 | 88 | 2 | 87 | 8.28e-42 | 132 |
| MsG0780040468.01.T01 | MTR_6g004250 | 72.059 | 68 | 19 | 0 | 7 | 74 | 5 | 72 | 1.10e-34 | 113 |
| MsG0780040468.01.T01 | MTR_3g116720 | 68.354 | 79 | 21 | 1 | 1 | 75 | 1 | 79 | 5.98e-34 | 112 |
| MsG0780040468.01.T01 | MTR_1g022290 | 59.302 | 86 | 29 | 1 | 6 | 85 | 1 | 86 | 5.68e-33 | 109 |
| MsG0780040468.01.T01 | MTR_5g020170 | 63.014 | 73 | 27 | 0 | 4 | 76 | 11 | 83 | 1.88e-30 | 103 |
| MsG0780040468.01.T01 | MTR_3g111880 | 65.217 | 69 | 24 | 0 | 6 | 74 | 1 | 69 | 1.19e-29 | 101 |
| MsG0780040468.01.T01 | MTR_8g077360 | 56.716 | 67 | 28 | 1 | 9 | 75 | 2 | 67 | 1.15e-24 | 88.2 |
| MsG0780040468.01.T01 | MTR_8g077380 | 56.716 | 67 | 28 | 1 | 9 | 75 | 2 | 67 | 1.29e-24 | 88.2 |
| MsG0780040468.01.T01 | MTR_8g077390 | 56.716 | 67 | 28 | 1 | 9 | 75 | 2 | 67 | 1.29e-24 | 88.2 |
| MsG0780040468.01.T01 | MTR_5g020540 | 60.317 | 63 | 24 | 1 | 10 | 72 | 2 | 63 | 1.24e-23 | 85.5 |
| MsG0780040468.01.T01 | MTR_8g077420 | 56.452 | 62 | 26 | 1 | 11 | 72 | 4 | 64 | 8.46e-23 | 83.6 |
| MsG0780040468.01.T01 | MTR_1g033600 | 50.000 | 78 | 39 | 0 | 1 | 78 | 1 | 78 | 1.91e-21 | 82.0 |
| MsG0780040468.01.T01 | MTR_3g111920 | 52.308 | 65 | 31 | 0 | 11 | 75 | 2 | 66 | 1.30e-20 | 78.2 |
| MsG0780040468.01.T01 | MTR_5g081860 | 54.930 | 71 | 32 | 0 | 12 | 82 | 27 | 97 | 1.62e-20 | 82.4 |
| MsG0780040468.01.T01 | MTR_8g063870 | 54.167 | 72 | 33 | 0 | 9 | 80 | 5 | 76 | 4.97e-19 | 78.6 |
| MsG0780040468.01.T01 | MTR_8g063600 | 50.667 | 75 | 37 | 0 | 6 | 80 | 4 | 78 | 7.89e-19 | 78.6 |
| MsG0780040468.01.T01 | MTR_3g462790 | 50.685 | 73 | 36 | 0 | 2 | 74 | 33 | 105 | 1.15e-18 | 78.2 |
| MsG0780040468.01.T01 | MTR_5g069710 | 47.826 | 69 | 36 | 0 | 11 | 79 | 28 | 96 | 6.37e-18 | 76.3 |
| MsG0780040468.01.T01 | MTR_7g067080 | 52.174 | 69 | 33 | 0 | 12 | 80 | 35 | 103 | 7.74e-18 | 75.9 |
| MsG0780040468.01.T01 | MTR_1g086180 | 57.627 | 59 | 24 | 1 | 11 | 68 | 10 | 68 | 7.28e-16 | 66.6 |
| MsG0780040468.01.T01 | MTR_1g111830 | 47.619 | 63 | 33 | 0 | 12 | 74 | 24 | 86 | 7.08e-15 | 67.4 |
| MsG0780040468.01.T01 | MTR_7g110580 | 55.000 | 60 | 26 | 1 | 11 | 69 | 7 | 66 | 3.42e-14 | 62.0 |
| MsG0780040468.01.T01 | MTR_4g111975 | 45.205 | 73 | 36 | 2 | 6 | 74 | 1 | 73 | 6.09e-14 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040468.01.T01 | AT1G75250 | 69.333 | 75 | 23 | 0 | 1 | 75 | 1 | 75 | 8.25e-36 | 117 |
| MsG0780040468.01.T01 | AT1G75250 | 60.440 | 91 | 31 | 1 | 1 | 86 | 1 | 91 | 1.05e-35 | 117 |
| MsG0780040468.01.T01 | AT2G21650 | 65.432 | 81 | 21 | 1 | 12 | 85 | 14 | 94 | 1.39e-34 | 114 |
| MsG0780040468.01.T01 | AT4G39250 | 62.651 | 83 | 25 | 1 | 12 | 88 | 14 | 96 | 5.31e-34 | 112 |
| MsG0780040468.01.T01 | AT1G19510 | 64.286 | 84 | 30 | 0 | 1 | 84 | 1 | 84 | 7.44e-29 | 99.8 |
| MsG0780040468.01.T01 | AT2G18328 | 62.295 | 61 | 23 | 0 | 15 | 75 | 14 | 74 | 1.80e-24 | 87.8 |
| MsG0780040468.01.T01 | AT5G05790 | 52.857 | 70 | 33 | 0 | 9 | 78 | 28 | 97 | 3.10e-20 | 82.0 |
| MsG0780040468.01.T01 | AT5G05790 | 52.857 | 70 | 33 | 0 | 9 | 78 | 28 | 97 | 3.10e-20 | 82.0 |
| MsG0780040468.01.T01 | AT4G36570 | 75.000 | 44 | 11 | 0 | 12 | 55 | 12 | 55 | 5.39e-20 | 76.3 |
| MsG0780040468.01.T01 | AT3G11280 | 52.239 | 67 | 32 | 0 | 12 | 78 | 33 | 99 | 1.14e-18 | 77.8 |
| MsG0780040468.01.T01 | AT3G11280 | 52.239 | 67 | 32 | 0 | 12 | 78 | 33 | 99 | 1.14e-18 | 77.8 |
| MsG0780040468.01.T01 | AT5G58900 | 50.000 | 74 | 34 | 1 | 4 | 74 | 23 | 96 | 1.57e-18 | 77.8 |
| MsG0780040468.01.T01 | AT2G38090 | 52.174 | 69 | 33 | 0 | 12 | 80 | 29 | 97 | 2.40e-18 | 77.4 |
| MsG0780040468.01.T01 | AT5G08520 | 47.297 | 74 | 39 | 0 | 1 | 74 | 1 | 74 | 5.19e-16 | 70.9 |
| MsG0780040468.01.T01 | AT1G49010 | 46.479 | 71 | 35 | 1 | 7 | 74 | 3 | 73 | 1.17e-14 | 67.4 |
| MsG0780040468.01.T01 | AT5G23650 | 46.377 | 69 | 37 | 0 | 6 | 74 | 7 | 75 | 1.36e-14 | 67.4 |
| MsG0780040468.01.T01 | AT5G04760 | 41.791 | 67 | 38 | 1 | 8 | 74 | 2 | 67 | 4.42e-13 | 62.0 |
| MsG0780040468.01.T01 | AT5G01200 | 42.424 | 66 | 37 | 1 | 10 | 74 | 28 | 93 | 1.76e-11 | 58.2 |
Find 15 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTAGTTCAATGTCAGCTTC+TGG | 0.381239 | 7:+78018003 | MsG0780040468.01.T01:CDS |
| GATATTAAACATATTGAGTC+AGG | 0.394581 | 7:+78018174 | MsG0780040468.01.T01:CDS |
| TAATGTTTCTTAACTTCCTC+AGG | 0.405406 | 7:-78018135 | None:intergenic |
| CAGTGTCTCATGAGGAGAAA+AGG | 0.467404 | 7:+78018229 | MsG0780040468.01.T01:CDS |
| CAATGTCAGCTTCTGGCTCA+TGG | 0.480309 | 7:+78018010 | MsG0780040468.01.T01:CDS |
| TTCTGGCTCATGGAGTGTTA+AGG | 0.488975 | 7:+78018020 | MsG0780040468.01.T01:CDS |
| GCTACATTGTACCAACGATC+TGG | 0.520197 | 7:-78018090 | None:intergenic |
| AACAGCTAAAGCTCTTTCAA+AGG | 0.549456 | 7:-78018052 | None:intergenic |
| ACATTATGAACTTCTTGTTG+AGG | 0.570098 | 7:+78018152 | MsG0780040468.01.T01:CDS |
| GAAAATTTCAGTGTCTCATG+AGG | 0.590467 | 7:+78018221 | MsG0780040468.01.T01:CDS |
| TACAATGTAGCTCATGCTGT+TGG | 0.593737 | 7:+78018102 | MsG0780040468.01.T01:CDS |
| AATGTAGCTCATGCTGTTGG+TGG | 0.602491 | 7:+78018105 | MsG0780040468.01.T01:CDS |
| ACAAAGACACTCCAGATCGT+TGG | 0.612332 | 7:+78018079 | MsG0780040468.01.T01:CDS |
| ATATTAAACATATTGAGTCA+GGG | 0.667795 | 7:+78018175 | MsG0780040468.01.T01:CDS |
| TGTTGGTGGCAAAACTCCTG+AGG | 0.702099 | 7:+78018119 | MsG0780040468.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATTAAACATATTGAGTCA+GGG | + | Chr7:78018175-78018194 | MsG0780040468.01.T01:CDS | 20.0% |
| !!! | ACTGAAATTTTCTTGTAATT+TGG | - | Chr7:78018213-78018232 | None:intergenic | 20.0% |
| !!! | ATTTTCTTGTAATTTGGAAA+TGG | - | Chr7:78018207-78018226 | None:intergenic | 20.0% |
| ! | GATATTAAACATATTGAGTC+AGG | + | Chr7:78018174-78018193 | MsG0780040468.01.T01:CDS | 25.0% |
| ACATTATGAACTTCTTGTTG+AGG | + | Chr7:78018152-78018171 | MsG0780040468.01.T01:CDS | 30.0% | |
| TAATGTTTCTTAACTTCCTC+AGG | - | Chr7:78018138-78018157 | None:intergenic | 30.0% | |
| AACAGCTAAAGCTCTTTCAA+AGG | - | Chr7:78018055-78018074 | None:intergenic | 35.0% | |
| GAAAATTTCAGTGTCTCATG+AGG | + | Chr7:78018221-78018240 | MsG0780040468.01.T01:CDS | 35.0% | |
| TACAATGTAGCTCATGCTGT+TGG | + | Chr7:78018102-78018121 | MsG0780040468.01.T01:CDS | 40.0% | |
| ! | TCTAGTTCAATGTCAGCTTC+TGG | + | Chr7:78018003-78018022 | MsG0780040468.01.T01:CDS | 40.0% |
| AATGTAGCTCATGCTGTTGG+TGG | + | Chr7:78018105-78018124 | MsG0780040468.01.T01:CDS | 45.0% | |
| ACAAAGACACTCCAGATCGT+TGG | + | Chr7:78018079-78018098 | MsG0780040468.01.T01:CDS | 45.0% | |
| CAGTGTCTCATGAGGAGAAA+AGG | + | Chr7:78018229-78018248 | MsG0780040468.01.T01:CDS | 45.0% | |
| GCTACATTGTACCAACGATC+TGG | - | Chr7:78018093-78018112 | None:intergenic | 45.0% | |
| !! | TTCTGGCTCATGGAGTGTTA+AGG | + | Chr7:78018020-78018039 | MsG0780040468.01.T01:CDS | 45.0% |
| !! | CAATGTCAGCTTCTGGCTCA+TGG | + | Chr7:78018010-78018029 | MsG0780040468.01.T01:CDS | 50.0% |
| !! | TGTTGGTGGCAAAACTCCTG+AGG | + | Chr7:78018119-78018138 | MsG0780040468.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 78017997 | 78018263 | 78017997 | ID=MsG0780040468.01;Name=MsG0780040468.01 |
| Chr7 | mRNA | 78017997 | 78018263 | 78017997 | ID=MsG0780040468.01.T01;Parent=MsG0780040468.01;Name=MsG0780040468.01.T01;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|1|1|0|88 |
| Chr7 | exon | 78017997 | 78018263 | 78017997 | ID=MsG0780040468.01.T01:exon:15511;Parent=MsG0780040468.01.T01 |
| Chr7 | CDS | 78017997 | 78018263 | 78017997 | ID=MsG0780040468.01.T01:cds;Parent=MsG0780040468.01.T01 |
| Gene Sequence |
| Protein sequence |
