Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041622.01.T01 | XP_013467909.2 | 89.041 | 73 | 8 | 0 | 1 | 73 | 38 | 110 | 3.54E-40 | 138 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041622.01.T01 | Q9SZL8 | 44.156 | 77 | 40 | 1 | 1 | 74 | 77 | 153 | 8.91E-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041622.01.T01 | A0A072VK17 | 89.041 | 73 | 8 | 0 | 1 | 73 | 38 | 110 | 2.59e-40 | 137 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0780041622.01.T01 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041622.01.T01 | MTR_1g057920 | 89.041 | 73 | 8 | 0 | 1 | 73 | 38 | 110 | 6.58e-44 | 137 |
| MsG0780041622.01.T01 | MTR_6g017200 | 80.822 | 73 | 14 | 0 | 1 | 73 | 62 | 134 | 1.42e-39 | 127 |
| MsG0780041622.01.T01 | MTR_2g070590 | 76.712 | 73 | 17 | 0 | 1 | 73 | 21 | 93 | 1.05e-36 | 119 |
| MsG0780041622.01.T01 | MTR_0402s0040 | 78.873 | 71 | 15 | 0 | 1 | 71 | 30 | 100 | 4.87e-34 | 120 |
| MsG0780041622.01.T01 | MTR_7g113010 | 75.342 | 73 | 4 | 1 | 1 | 73 | 461 | 519 | 1.42e-30 | 112 |
| MsG0780041622.01.T01 | MTR_5g015480 | 67.606 | 71 | 23 | 0 | 1 | 71 | 60 | 130 | 6.09e-28 | 101 |
| MsG0780041622.01.T01 | MTR_0137s0040 | 67.692 | 65 | 21 | 0 | 1 | 65 | 40 | 104 | 4.00e-26 | 92.8 |
| MsG0780041622.01.T01 | MTR_0291s0060 | 74.468 | 47 | 12 | 0 | 1 | 47 | 39 | 85 | 2.89e-19 | 74.7 |
| MsG0780041622.01.T01 | MTR_1g091013 | 58.929 | 56 | 23 | 0 | 10 | 65 | 14 | 69 | 1.78e-16 | 67.0 |
| MsG0780041622.01.T01 | MTR_4g102720 | 45.070 | 71 | 38 | 1 | 1 | 71 | 11 | 80 | 5.16e-16 | 69.3 |
| MsG0780041622.01.T01 | MTR_4g067290 | 48.571 | 70 | 35 | 1 | 2 | 71 | 61 | 129 | 4.10e-14 | 64.3 |
| MsG0780041622.01.T01 | MTR_4g067290 | 48.571 | 70 | 35 | 1 | 2 | 71 | 81 | 149 | 5.64e-14 | 64.3 |
| MsG0780041622.01.T01 | MTR_4g067290 | 48.571 | 70 | 35 | 1 | 2 | 71 | 81 | 149 | 5.68e-14 | 64.3 |
| MsG0780041622.01.T01 | MTR_4g067290 | 48.571 | 70 | 35 | 1 | 2 | 71 | 79 | 147 | 5.95e-14 | 64.3 |
| MsG0780041622.01.T01 | MTR_4g067290 | 48.571 | 70 | 35 | 1 | 2 | 71 | 121 | 189 | 6.03e-14 | 64.7 |
| MsG0780041622.01.T01 | MTR_4g067290 | 48.571 | 70 | 35 | 1 | 2 | 71 | 61 | 129 | 2.63e-13 | 62.4 |
| MsG0780041622.01.T01 | MTR_2g098920 | 47.143 | 70 | 36 | 1 | 2 | 71 | 85 | 153 | 8.03e-13 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041622.01.T01 | AT3G59470 | 47.143 | 70 | 36 | 1 | 2 | 71 | 87 | 155 | 7.31e-13 | 61.6 |
| MsG0780041622.01.T01 | AT3G59470 | 47.143 | 70 | 36 | 1 | 2 | 71 | 75 | 143 | 7.92e-13 | 61.2 |
| MsG0780041622.01.T01 | AT3G59470 | 47.143 | 70 | 36 | 1 | 2 | 71 | 75 | 143 | 7.92e-13 | 61.2 |
| MsG0780041622.01.T01 | AT4G38180 | 44.156 | 77 | 40 | 1 | 1 | 74 | 77 | 153 | 9.08e-12 | 58.9 |
Find 13 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTCGTTGTGCATCCTGTTC+TGG | 0.279946 | 7:+93177018 | None:intergenic |
| TTTGGTATTCGTCGAGAATA+TGG | 0.287007 | 7:-93177154 | MsG0780041622.01.T01:CDS |
| AATGATTACAGGAAAAGAAT+TGG | 0.325772 | 7:-93177178 | MsG0780041622.01.T01:CDS |
| TACAGGAAAAGAATTGGATT+TGG | 0.343860 | 7:-93177172 | MsG0780041622.01.T01:CDS |
| GGAAATAAAAGCAGAATGGA+TGG | 0.493838 | 7:-93177133 | MsG0780041622.01.T01:CDS |
| TCTAGAGCAGAAACCAGAAC+AGG | 0.523760 | 7:-93177031 | MsG0780041622.01.T01:CDS |
| TCTGCTCTAGACTCCGTCGT+CGG | 0.570230 | 7:+93177043 | None:intergenic |
| ATATGGAAATAAAAGCAGAA+TGG | 0.588771 | 7:-93177137 | MsG0780041622.01.T01:CDS |
| GAAGTTTGAATCTGAGACGG+CGG | 0.620676 | 7:-93177215 | MsG0780041622.01.T01:CDS |
| CAAAAGACGTCAACCGACGA+CGG | 0.636965 | 7:-93177056 | MsG0780041622.01.T01:CDS |
| AATGAAGTTTGAATCTGAGA+CGG | 0.638568 | 7:-93177218 | MsG0780041622.01.T01:CDS |
| AAGATTCACATGCTTCAAAG+AGG | 0.642884 | 7:-93177095 | MsG0780041622.01.T01:CDS |
| AGATTCACATGCTTCAAAGA+GGG | 0.654356 | 7:-93177094 | MsG0780041622.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATGAATTTTATAATGATTAC+AGG | - | Chr7:93177040-93177059 | MsG0780041622.01.T01:CDS | 15.0% |
| ! | AATGATTACAGGAAAAGAAT+TGG | - | Chr7:93177051-93177070 | MsG0780041622.01.T01:CDS | 25.0% |
| ! | ATATGGAAATAAAAGCAGAA+TGG | - | Chr7:93177092-93177111 | MsG0780041622.01.T01:CDS | 25.0% |
| TACAGGAAAAGAATTGGATT+TGG | - | Chr7:93177057-93177076 | MsG0780041622.01.T01:CDS | 30.0% | |
| AAGATTCACATGCTTCAAAG+AGG | - | Chr7:93177134-93177153 | MsG0780041622.01.T01:CDS | 35.0% | |
| AGATTCACATGCTTCAAAGA+GGG | - | Chr7:93177135-93177154 | MsG0780041622.01.T01:CDS | 35.0% | |
| GGAAATAAAAGCAGAATGGA+TGG | - | Chr7:93177096-93177115 | MsG0780041622.01.T01:CDS | 35.0% | |
| TTTGGTATTCGTCGAGAATA+TGG | - | Chr7:93177075-93177094 | MsG0780041622.01.T01:CDS | 35.0% | |
| ! | GAAGTTTGAATCTGAGACGG+CGG | - | Chr7:93177014-93177033 | MsG0780041622.01.T01:CDS | 45.0% |
| ! | TCTAGAGCAGAAACCAGAAC+AGG | - | Chr7:93177198-93177217 | MsG0780041622.01.T01:CDS | 45.0% |
| ACTCGTTGTGCATCCTGTTC+TGG | + | Chr7:93177214-93177233 | None:intergenic | 50.0% | |
| CAAAAGACGTCAACCGACGA+CGG | - | Chr7:93177173-93177192 | MsG0780041622.01.T01:CDS | 50.0% | |
| ! | TCTGCTCTAGACTCCGTCGT+CGG | + | Chr7:93177189-93177208 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 93177012 | 93177239 | 93177012 | ID=MsG0780041622.01;Name=MsG0780041622.01 |
| Chr7 | mRNA | 93177012 | 93177239 | 93177012 | ID=MsG0780041622.01.T01;Parent=MsG0780041622.01;Name=MsG0780041622.01.T01;_AED=0.52;_eAED=0.52;_QI=0|-1|0|1|-1|0|1|0|75 |
| Chr7 | exon | 93177012 | 93177239 | 93177012 | ID=MsG0780041622.01.T01:exon:20451;Parent=MsG0780041622.01.T01 |
| Chr7 | CDS | 93177012 | 93177239 | 93177012 | ID=MsG0780041622.01.T01:cds;Parent=MsG0780041622.01.T01 |
| Gene Sequence |
| Protein sequence |
