AlfalfaGEDB | MsG0880042440.01

M. sativa cultivar ZhongmuNo.1 / MsG0880042440.01

KEGG: K09422
GO: GO:0000786 GO:0003677 GO:0005634 GO:0006334
PFAM: PF00249 PF00538

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042440.01.T01	XP_003627170.1	81.053	190	23	3	1	190	1	177	2.16E-96	291

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042440.01.T01	F4IEY4	49.479	192	85	3	1	192	1	180	2.54E-51	169

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042440.01.T01	G7LI96	81.053	190	23	3	1	190	1	177	1.03e-96	291

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TFs/TRs:

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Gene ID	Type	Classification
MsG0880042440.01.T01	TF	MYB-related

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042440.01.T01	MTR_8g018410	81.053	190	23	3	1	190	1	177	2.62e-100	291
MsG0880042440.01.T01	MTR_2g099610	50.990	202	86	5	1	191	1	200	2.51e-55	177
MsG0880042440.01.T01	MTR_8g494240	41.667	192	98	4	1	191	1	179	2.78e-35	126
MsG0880042440.01.T01	MTR_8g494240	41.667	192	98	4	1	191	1	179	3.55e-35	125
MsG0880042440.01.T01	MTR_5g015340	39.691	194	99	4	1	192	1	178	9.22e-35	124
MsG0880042440.01.T01	MTR_5g015340	39.691	194	99	4	1	192	1	178	9.22e-35	124
MsG0880042440.01.T01	MTR_8g494240	41.667	192	98	4	1	191	1	179	3.22e-34	125
MsG0880042440.01.T01	MTR_2g031870	41.361	191	102	5	1	191	1	181	8.79e-34	122
MsG0880042440.01.T01	MTR_5g015340	39.691	194	99	4	1	192	1	178	9.16e-34	124
MsG0880042440.01.T01	MTR_4g097130	39.000	200	104	5	1	188	1	194	4.69e-33	120

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042440.01.T01	AT1G72740	49.479	192	85	3	1	192	1	180	2.59e-52	169
MsG0880042440.01.T01	AT1G72740	49.215	191	85	3	1	191	1	179	4.12e-52	169
MsG0880042440.01.T01	AT1G17520	50.000	196	85	2	1	192	1	187	3.88e-46	154
MsG0880042440.01.T01	AT1G17520	49.744	195	85	2	1	191	1	186	1.47e-45	152
MsG0880042440.01.T01	AT1G17520	49.746	197	85	3	1	192	1	188	2.53e-44	149
MsG0880042440.01.T01	AT1G17520	49.490	196	85	3	1	191	1	187	8.81e-44	147
MsG0880042440.01.T01	AT1G49950	38.342	193	101	6	1	191	1	177	7.50e-31	114
MsG0880042440.01.T01	AT1G49950	38.342	193	101	6	1	191	1	177	7.50e-31	114
MsG0880042440.01.T01	AT1G49950	38.342	193	101	6	1	191	1	177	7.50e-31	114
MsG0880042440.01.T01	AT3G49850	35.233	193	107	3	1	190	1	178	1.40e-27	105

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Find 38 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGACATGGCCCTGGTAAA+TGG	0.273347	8:-8733336	MsG0880042440.01.T01:CDS
AACCTCAATGAAACTAGCAA+TGG	0.624133	8:+8731326	None:intergenic
AAGGACCGAACTGAGGGTCA+AGG	0.625130	8:+8733302	None:intergenic
ACAGGAGCAGCAGCAGGAAC+AGG	0.428758	8:+8733078	None:intergenic
ACTACATTAAAGGATACTAA+TGG	0.429817	8:-8731363	MsG0880042440.01.T01:CDS
ACTAGCAATGGCATTTACGT+CGG	0.664174	8:+8731338	None:intergenic
AGAATGGCTTTCCATTTACC+AGG	0.481104	8:+8733325	None:intergenic
AGCAGCAGCAGGTTTGCATT+TGG	0.345557	8:+8733113	None:intergenic
AGTCACTGACTGCAAGGTTG+AGG	0.520186	8:-8730992	MsG0880042440.01.T01:CDS
ATAATATTACTCAAGTTCCT+AGG	0.465364	8:-8733140	MsG0880042440.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATAAACACACGCACTAAA+GGG	+	Chr8:8731823-8731842	None:intergenic	30.0%
CTTTACATTTATTGGATGAC+TGG	-	Chr8:8732406-8732425	MsG0880042440.01.T01:intron	30.0%
GATTTATAATGTTGTTGTCG+CGG	-	Chr8:8732014-8732033	MsG0880042440.01.T01:intron	30.0%
TTAACAACAACATCAGAAGA+AGG	+	Chr8:8731338-8731357	None:intergenic	30.0%
TTGTTAAAAACAACACACCT+AGG	-	Chr8:8731414-8731433	MsG0880042440.01.T01:intron	30.0%
TTGTTAAATAGCAGCTATAG+AGG	+	Chr8:8732192-8732211	None:intergenic	30.0%
AAAAACGAAAACCCTAAGCT+AGG	+	Chr8:8731116-8731135	None:intergenic	35.0%
AAAACCGTCTTAAAAGGAGA+GGG	+	Chr8:8731967-8731986	None:intergenic	35.0%
AAAACGAAAACCCTAAGCTA+GGG	+	Chr8:8731115-8731134	None:intergenic	35.0%
AACCTCAATGAAACTAGCAA+TGG	+	Chr8:8733027-8733046	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	8730953	8733419	8730953	ID=MsG0880042440.01;Name=MsG0880042440.01
Chr8	mRNA	8730953	8733419	8730953	ID=MsG0880042440.01.T01;Parent=MsG0880042440.01;Name=MsG0880042440.01.T01;_AED=0.48;_eAED=0.48;_QI=0\|0\|0\|1\|1\|1\|4\|0\|192
Chr8	exon	8733270	8733419	8733270	ID=MsG0880042440.01.T01:exon:2818;Parent=MsG0880042440.01.T01
Chr8	exon	8732937	8733187	8732937	ID=MsG0880042440.01.T01:exon:2817;Parent=MsG0880042440.01.T01
Chr8	exon	8731329	8731413	8731329	ID=MsG0880042440.01.T01:exon:2816;Parent=MsG0880042440.01.T01
Chr8	exon	8730953	8731045	8730953	ID=MsG0880042440.01.T01:exon:2815;Parent=MsG0880042440.01.T01
Chr8	CDS	8733270	8733419	8733270	ID=MsG0880042440.01.T01:cds;Parent=MsG0880042440.01.T01
Chr8	CDS	8732937	8733187	8732937	ID=MsG0880042440.01.T01:cds;Parent=MsG0880042440.01.T01
Chr8	CDS	8731329	8731413	8731329	ID=MsG0880042440.01.T01:cds;Parent=MsG0880042440.01.T01
Chr8	CDS	8730953	8731045	8730953	ID=MsG0880042440.01.T01:cds;Parent=MsG0880042440.01.T01

Gene Sequence

>MsG0880042440.01.T01

ATGGGAAATCAAAAGCTGAAATGGACGAATGAAGAAGAAGACGCTCTTATCGCTGGAATCGAAAGACATGGCCCTGGTAAATGGAAAGCCATTCTCCTTGACCCTCAGTTCGGTCCTTTACTCACTTCTCGTTCCAACATCGACCTCAAGGATAAATGGAGGAATATGAATGTGAATAATATTACTCAAGTTCCTAGGTTTCCCAAATGCAAACCTGCTGCTGCTGCTGCTTCTTCTCCTGTTCCTGCTGCTGCTCCTGTTTCTTCTTCCAGTGCTGCTGTTAATCAAAATGTTGCTCTTCCTTCTTCTGATGTTGTTGTTAACAATGTTACTCCTCCTCTTCAAATTGTTCCTCCTCCTCTTCAAAATGACCAAGATGTTGTTAAAAACAACACACCTAGGTATGATGTCATGATTTATGAAGCTCTGACTACATTAAAGGATACTAATGGATCCGACGTAAATGCCATTGCTAGTTTCATTGAGCAAAAGCATCCGGTTCCTCAAAATTTCAGAAAGTCACTGACTGCAAGGTTGAGGATGCTTGTGAGTCAAGGGAAGCTTGAAAAGGTAAATTAA

Protein sequence

>MsG0880042440.01.T01

MGNQKLKWTNEEEDALIAGIERHGPGKWKAILLDPQFGPLLTSRSNIDLKDKWRNMNVNNITQVPRFPKCKPAAAAASSPVPAAAPVSSSSAAVNQNVALPSSDVVVNNVTPPLQIVPPPLQNDQDVVKNNTPRYDVMIYEALTTLKDTNGSDVNAIASFIEQKHPVPQNFRKSLTARLRMLVSQGKLEKVN*