Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042598.01.T01 | RHN39330.1 | 86.567 | 67 | 9 | 0 | 5 | 71 | 160 | 226 | 1.54E-31 | 121 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042598.01.T01 | P27347 | 47.222 | 72 | 35 | 1 | 21 | 92 | 31 | 99 | 1.40E-15 | 70.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042598.01.T01 | A0A396GDP9 | 86.567 | 67 | 9 | 0 | 5 | 71 | 160 | 226 | 7.33e-32 | 121 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0880042598.01.T01 | TR | HMG |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042598.01.T01 | MTR_8g018100 | 86.567 | 67 | 9 | 0 | 5 | 71 | 180 | 246 | 4.20e-35 | 120 |
| MsG0880042598.01.T01 | MTR_1g115220 | 80.519 | 77 | 15 | 0 | 5 | 81 | 76 | 152 | 2.30e-30 | 107 |
| MsG0880042598.01.T01 | MTR_3g115580 | 47.826 | 69 | 36 | 0 | 18 | 86 | 32 | 100 | 2.79e-16 | 70.1 |
| MsG0880042598.01.T01 | MTR_7g068280 | 47.458 | 59 | 31 | 0 | 27 | 85 | 47 | 105 | 1.62e-13 | 63.2 |
| MsG0880042598.01.T01 | MTR_5g024180 | 38.554 | 83 | 44 | 2 | 15 | 93 | 16 | 95 | 1.31e-12 | 60.5 |
| MsG0880042598.01.T01 | MTR_8g073345 | 40.278 | 72 | 38 | 1 | 15 | 81 | 16 | 87 | 3.88e-12 | 58.9 |
| MsG0880042598.01.T01 | MTR_8g073345 | 40.278 | 72 | 38 | 1 | 15 | 81 | 16 | 87 | 5.42e-12 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042598.01.T01 | AT4G35570 | 47.692 | 65 | 34 | 0 | 22 | 86 | 25 | 89 | 1.06e-15 | 67.8 |
| MsG0880042598.01.T01 | AT4G35570 | 47.692 | 65 | 34 | 0 | 22 | 86 | 25 | 89 | 1.06e-15 | 67.8 |
| MsG0880042598.01.T01 | AT3G51880 | 41.558 | 77 | 44 | 1 | 5 | 81 | 28 | 103 | 1.11e-12 | 60.8 |
| MsG0880042598.01.T01 | AT1G20696 | 44.928 | 69 | 37 | 1 | 13 | 81 | 18 | 85 | 1.18e-12 | 60.5 |
| MsG0880042598.01.T01 | AT3G51880 | 41.558 | 77 | 44 | 1 | 5 | 81 | 28 | 103 | 1.62e-12 | 60.8 |
| MsG0880042598.01.T01 | AT1G20693 | 42.029 | 69 | 39 | 1 | 19 | 86 | 25 | 93 | 1.81e-12 | 60.1 |
| MsG0880042598.01.T01 | AT1G20693 | 42.029 | 69 | 39 | 1 | 19 | 86 | 25 | 93 | 1.84e-12 | 60.1 |
| MsG0880042598.01.T01 | AT3G51880 | 41.558 | 77 | 44 | 1 | 5 | 81 | 28 | 103 | 2.10e-12 | 60.5 |
| MsG0880042598.01.T01 | AT3G51880 | 41.558 | 77 | 44 | 1 | 5 | 81 | 28 | 103 | 2.10e-12 | 60.5 |
| MsG0880042598.01.T01 | AT3G51880 | 41.558 | 77 | 44 | 1 | 5 | 81 | 28 | 103 | 2.10e-12 | 60.5 |
| MsG0880042598.01.T01 | AT1G20696 | 44.928 | 69 | 37 | 1 | 13 | 81 | 18 | 85 | 2.58e-12 | 59.7 |
| MsG0880042598.01.T01 | AT1G20696 | 44.928 | 69 | 37 | 1 | 13 | 81 | 18 | 85 | 2.99e-12 | 59.3 |
| MsG0880042598.01.T01 | AT1G20693 | 42.029 | 69 | 39 | 1 | 19 | 86 | 25 | 93 | 4.88e-12 | 58.9 |
| MsG0880042598.01.T01 | AT2G17560 | 40.299 | 67 | 39 | 1 | 22 | 87 | 25 | 91 | 1.25e-11 | 57.8 |
| MsG0880042598.01.T01 | AT2G17560 | 40.299 | 67 | 39 | 1 | 22 | 87 | 25 | 91 | 1.40e-11 | 57.8 |
| MsG0880042598.01.T01 | AT2G17560 | 40.299 | 67 | 39 | 1 | 22 | 87 | 25 | 91 | 1.40e-11 | 57.8 |
Find 23 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCTCAAACGATTGCAATTT+TGG | 0.019785 | 8:-11256880 | None:intergenic |
| GAGGGATTTAAGAGGCAATT+TGG | 0.295975 | 8:+11256898 | MsG0880042598.01.T01:CDS |
| TAAAGTTGGGAAAGGGAAAA+AGG | 0.356050 | 8:+11256386 | MsG0880042598.01.T01:CDS |
| CCAAAATTGCAATCGTTTGA+GGG | 0.399456 | 8:+11256880 | MsG0880042598.01.T01:CDS |
| ATCTTGATTCAAAGGATGTT+AGG | 0.404379 | 8:+11256579 | MsG0880042598.01.T01:CDS |
| TCCAAAATTGCAATCGTTTG+AGG | 0.414425 | 8:+11256879 | MsG0880042598.01.T01:CDS |
| ACTTTAGTCCGTTTCGCAGT+TGG | 0.458641 | 8:-11256369 | None:intergenic |
| TACAAGACTTGCAAAGAGAG+TGG | 0.462794 | 8:-11256938 | None:intergenic |
| GGAAGCTAATCTTGATTCAA+AGG | 0.468919 | 8:+11256571 | MsG0880042598.01.T01:CDS |
| ACTGCGAAACGGACTAAAGT+TGG | 0.477591 | 8:+11256372 | MsG0880042598.01.T01:CDS |
| AATCGTTTGAGGGATTTAAG+AGG | 0.511108 | 8:+11256890 | MsG0880042598.01.T01:CDS |
| CTGCGAAACGGACTAAAGTT+GGG | 0.511483 | 8:+11256373 | MsG0880042598.01.T01:CDS |
| GTAAGGAAGATTGCGAGAAG+TGG | 0.541777 | 8:+11256708 | MsG0880042598.01.T01:CDS |
| ATGAAAAGAAAGAAATCAGT+TGG | 0.544749 | 8:-11256438 | None:intergenic |
| ACAAGACTTGCAAAGAGAGT+GGG | 0.547118 | 8:-11256937 | None:intergenic |
| CAAAAGAACCAACTGCGAAA+CGG | 0.556686 | 8:+11256361 | MsG0880042598.01.T01:CDS |
| GAAGTGGAGGTCTTTGACTG+AGG | 0.557902 | 8:+11256724 | MsG0880042598.01.T01:CDS |
| TTGATTCAAAGGATGTTAGG+AGG | 0.570900 | 8:+11256582 | MsG0880042598.01.T01:CDS |
| AACGGACTAAAGTTGGGAAA+GGG | 0.587037 | 8:+11256379 | MsG0880042598.01.T01:CDS |
| AAACGGACTAAAGTTGGGAA+AGG | 0.588435 | 8:+11256378 | MsG0880042598.01.T01:CDS |
| TGATTCAAAGGATGTTAGGA+GGG | 0.600809 | 8:+11256583 | MsG0880042598.01.T01:CDS |
| AGGAAGATTGCGAGAAGTGG+AGG | 0.614149 | 8:+11256711 | MsG0880042598.01.T01:CDS |
| AAAGGTTAAGGATTCTAATG+CGG | 0.688426 | 8:+11256404 | MsG0880042598.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGATATGGTTAAAATTGTTT+AGG | + | Chr8:11256832-11256851 | MsG0880042598.01.T01:intron | 20.0% |
| !!! | ATTTCTTTCTTTTCATGTAA+GGG | + | Chr8:11256445-11256464 | MsG0880042598.01.T01:intron | 20.0% |
| !!! | TGTTTTGATTAACTTATCTA+AGG | + | Chr8:11256642-11256661 | MsG0880042598.01.T01:intron | 20.0% |
| ! | ATGAAAAGAAAGAAATCAGT+TGG | - | Chr8:11256441-11256460 | None:intergenic | 25.0% |
| ! | ATGTAATGCAAAAACTAGAT+TGG | + | Chr8:11256686-11256705 | MsG0880042598.01.T01:intron | 25.0% |
| ! | TTGTTTAGTTGAATTGTGTT+TGG | + | Chr8:11256616-11256635 | MsG0880042598.01.T01:intron | 25.0% |
| !! | GATTTCTTTCTTTTCATGTA+AGG | + | Chr8:11256444-11256463 | MsG0880042598.01.T01:intron | 25.0% |
| !! | TGAGTTTAGAAAGACTTTTA+AGG | + | Chr8:11256550-11256569 | MsG0880042598.01.T01:CDS | 25.0% |
| AAAGGTTAAGGATTCTAATG+CGG | + | Chr8:11256404-11256423 | MsG0880042598.01.T01:CDS | 30.0% | |
| ATCTTGATTCAAAGGATGTT+AGG | + | Chr8:11256579-11256598 | MsG0880042598.01.T01:CDS | 30.0% | |
| ATGCAAAAACTAGATTGGTA+AGG | + | Chr8:11256691-11256710 | MsG0880042598.01.T01:intron | 30.0% | |
| TGCACCAAATTCAATCAAAA+CGG | - | Chr8:11256777-11256796 | None:intergenic | 30.0% | |
| ! | TTTTGGACTTGCATCTTAAA+AGG | - | Chr8:11256866-11256885 | None:intergenic | 30.0% |
| !!! | TGTTCCGTTTTGATTGAATT+TGG | + | Chr8:11256770-11256789 | MsG0880042598.01.T01:intron | 30.0% |
| !!! | TTGTTTGTGCTGTTTTGTAA+TGG | + | Chr8:11256474-11256493 | MsG0880042598.01.T01:intron | 30.0% |
| AATCGTTTGAGGGATTTAAG+AGG | + | Chr8:11256890-11256909 | MsG0880042598.01.T01:CDS | 35.0% | |
| CCAAAATTGCAATCGTTTGA+GGG | + | Chr8:11256880-11256899 | MsG0880042598.01.T01:CDS | 35.0% | |
| GGAAGCTAATCTTGATTCAA+AGG | + | Chr8:11256571-11256590 | MsG0880042598.01.T01:CDS | 35.0% | |
| GTGTTATGTTTATGTGATGC+TGG | + | Chr8:11256799-11256818 | MsG0880042598.01.T01:intron | 35.0% | |
| TAAAGTTGGGAAAGGGAAAA+AGG | + | Chr8:11256386-11256405 | MsG0880042598.01.T01:CDS | 35.0% | |
| TCCAAAATTGCAATCGTTTG+AGG | + | Chr8:11256879-11256898 | MsG0880042598.01.T01:CDS | 35.0% | |
| TGATTCAAAGGATGTTAGGA+GGG | + | Chr8:11256583-11256602 | MsG0880042598.01.T01:CDS | 35.0% | |
| TTGATTCAAAGGATGTTAGG+AGG | + | Chr8:11256582-11256601 | MsG0880042598.01.T01:CDS | 35.0% | |
| ! | TTTATGTGATGCTGGTCTAA+TGG | + | Chr8:11256807-11256826 | MsG0880042598.01.T01:intron | 35.0% |
| !! | GATTGGTTTGATTTGTTGCA+GGG | + | Chr8:11256526-11256545 | MsG0880042598.01.T01:intron | 35.0% |
| !! | GCAGTTGGTTCTTTTGAATT+TGG | - | Chr8:11256357-11256376 | None:intergenic | 35.0% |
| !! | GGTTTGGTTCTATAGTTTGA+GGG | + | Chr8:11256495-11256514 | MsG0880042598.01.T01:intron | 35.0% |
| !! | TGATTGGTTTGATTTGTTGC+AGG | + | Chr8:11256525-11256544 | MsG0880042598.01.T01:intron | 35.0% |
| !! | TGGTTTGGTTCTATAGTTTG+AGG | + | Chr8:11256494-11256513 | MsG0880042598.01.T01:intron | 35.0% |
| !!! | GTTTGAGGGTTTTAGTTGAT+TGG | + | Chr8:11256509-11256528 | MsG0880042598.01.T01:intron | 35.0% |
| !!! | TGTGCTGTTTTGTAATGGTT+TGG | + | Chr8:11256479-11256498 | MsG0880042598.01.T01:intron | 35.0% |
| ACAAGACTTGCAAAGAGAGT+GGG | - | Chr8:11256940-11256959 | None:intergenic | 40.0% | |
| CAAAAGAACCAACTGCGAAA+CGG | + | Chr8:11256361-11256380 | MsG0880042598.01.T01:CDS | 40.0% | |
| GAGGGATTTAAGAGGCAATT+TGG | + | Chr8:11256898-11256917 | MsG0880042598.01.T01:CDS | 40.0% | |
| TACAAGACTTGCAAAGAGAG+TGG | - | Chr8:11256941-11256960 | None:intergenic | 40.0% | |
| TGGGAAAGGGAAAAAGGTTA+AGG | + | Chr8:11256392-11256411 | MsG0880042598.01.T01:CDS | 40.0% | |
| ! | CCCTCAAACGATTGCAATTT+TGG | - | Chr8:11256883-11256902 | None:intergenic | 40.0% |
| ! | GCTGGTCTAATGGTATGATA+TGG | + | Chr8:11256817-11256836 | MsG0880042598.01.T01:intron | 40.0% |
| !! | AAACGGACTAAAGTTGGGAA+AGG | + | Chr8:11256378-11256397 | MsG0880042598.01.T01:CDS | 40.0% |
| !! | AACGGACTAAAGTTGGGAAA+GGG | + | Chr8:11256379-11256398 | MsG0880042598.01.T01:CDS | 40.0% |
| ACTTTAGTCCGTTTCGCAGT+TGG | - | Chr8:11256372-11256391 | None:intergenic | 45.0% | |
| GTAAGGAAGATTGCGAGAAG+TGG | + | Chr8:11256708-11256727 | MsG0880042598.01.T01:CDS | 45.0% | |
| !! | ACTGCGAAACGGACTAAAGT+TGG | + | Chr8:11256372-11256391 | MsG0880042598.01.T01:CDS | 45.0% |
| !! | CTGCGAAACGGACTAAAGTT+GGG | + | Chr8:11256373-11256392 | MsG0880042598.01.T01:CDS | 45.0% |
| AGGAAGATTGCGAGAAGTGG+AGG | + | Chr8:11256711-11256730 | MsG0880042598.01.T01:CDS | 50.0% | |
| GAAGTGGAGGTCTTTGACTG+AGG | + | Chr8:11256724-11256743 | MsG0880042598.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 11256336 | 11256961 | 11256336 | ID=MsG0880042598.01;Name=MsG0880042598.01 |
| Chr8 | mRNA | 11256336 | 11256961 | 11256336 | ID=MsG0880042598.01.T01;Parent=MsG0880042598.01;Name=MsG0880042598.01.T01;_AED=0.66;_eAED=0.66;_QI=0|0|0|0.75|1|1|4|0|111 |
| Chr8 | exon | 11256336 | 11256460 | 11256336 | ID=MsG0880042598.01.T01:exon:36482;Parent=MsG0880042598.01.T01 |
| Chr8 | exon | 11256547 | 11256604 | 11256547 | ID=MsG0880042598.01.T01:exon:36483;Parent=MsG0880042598.01.T01 |
| Chr8 | exon | 11256704 | 11256748 | 11256704 | ID=MsG0880042598.01.T01:exon:36484;Parent=MsG0880042598.01.T01 |
| Chr8 | exon | 11256854 | 11256961 | 11256854 | ID=MsG0880042598.01.T01:exon:36485;Parent=MsG0880042598.01.T01 |
| Chr8 | CDS | 11256336 | 11256460 | 11256336 | ID=MsG0880042598.01.T01:cds;Parent=MsG0880042598.01.T01 |
| Chr8 | CDS | 11256547 | 11256604 | 11256547 | ID=MsG0880042598.01.T01:cds;Parent=MsG0880042598.01.T01 |
| Chr8 | CDS | 11256704 | 11256748 | 11256704 | ID=MsG0880042598.01.T01:cds;Parent=MsG0880042598.01.T01 |
| Chr8 | CDS | 11256854 | 11256961 | 11256854 | ID=MsG0880042598.01.T01:cds;Parent=MsG0880042598.01.T01 |
| Gene Sequence |
| Protein sequence |
