Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042874.01.T01 | PNX59517.1 | 76.596 | 47 | 11 | 0 | 1 | 47 | 1 | 47 | 2.64E-17 | 78.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042874.01.T01 | Q9SCK6 | 51.064 | 47 | 23 | 0 | 1 | 47 | 13 | 59 | 1.66E-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042874.01.T01 | A0A2K3JZP1 | 76.596 | 47 | 11 | 0 | 1 | 47 | 1 | 47 | 1.26e-17 | 78.6 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0880042874.01.T01 | TF | NAC |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042874.01.T01 | MTR_8g023880 | 68.000 | 50 | 15 | 1 | 1 | 49 | 7 | 56 | 6.78e-17 | 71.6 |
| MsG0880042874.01.T01 | MTR_8g023840 | 64.151 | 53 | 17 | 2 | 1 | 51 | 11 | 63 | 6.28e-16 | 69.7 |
| MsG0880042874.01.T01 | MTR_8g023860 | 60.377 | 53 | 20 | 1 | 1 | 52 | 12 | 64 | 3.88e-15 | 67.8 |
| MsG0880042874.01.T01 | MTR_8g023930 | 54.717 | 53 | 23 | 1 | 1 | 52 | 24 | 76 | 8.50e-13 | 60.8 |
| MsG0880042874.01.T01 | MTR_8g023900 | 50.943 | 53 | 25 | 1 | 1 | 52 | 12 | 64 | 9.19e-13 | 60.8 |
| MsG0880042874.01.T01 | MTR_8g023900 | 54.902 | 51 | 22 | 1 | 3 | 52 | 195 | 245 | 4.35e-12 | 58.9 |
| MsG0880042874.01.T01 | MTR_5g069030 | 53.846 | 52 | 24 | 0 | 1 | 52 | 25 | 76 | 1.85e-12 | 60.1 |
| MsG0880042874.01.T01 | MTR_3g093040 | 48.936 | 47 | 24 | 0 | 1 | 47 | 108 | 154 | 2.86e-11 | 56.6 |
| MsG0880042874.01.T01 | MTR_3g093050 | 48.936 | 47 | 24 | 0 | 1 | 47 | 22 | 68 | 8.23e-11 | 55.5 |
| MsG0880042874.01.T01 | MTR_3g093050 | 48.936 | 47 | 24 | 0 | 1 | 47 | 22 | 68 | 8.24e-11 | 55.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042874.01.T01 | AT3G49530 | 51.064 | 47 | 23 | 0 | 1 | 47 | 13 | 59 | 1.70e-12 | 60.1 |
| MsG0880042874.01.T01 | AT3G49530 | 51.064 | 47 | 23 | 0 | 1 | 47 | 13 | 59 | 3.33e-12 | 59.3 |
| MsG0880042874.01.T01 | AT5G24590 | 51.064 | 47 | 23 | 0 | 1 | 47 | 13 | 59 | 9.56e-12 | 58.2 |
| MsG0880042874.01.T01 | AT1G33060 | 46.154 | 52 | 28 | 0 | 1 | 52 | 24 | 75 | 6.90e-11 | 55.8 |
| MsG0880042874.01.T01 | AT1G33060 | 46.154 | 52 | 28 | 0 | 1 | 52 | 24 | 75 | 6.90e-11 | 55.8 |
| MsG0880042874.01.T01 | AT4G35580 | 46.154 | 52 | 28 | 0 | 1 | 52 | 9 | 60 | 8.26e-11 | 55.5 |
| MsG0880042874.01.T01 | AT4G35580 | 46.154 | 52 | 28 | 0 | 1 | 52 | 9 | 60 | 8.27e-11 | 55.5 |
| MsG0880042874.01.T01 | AT4G35580 | 46.154 | 52 | 28 | 0 | 1 | 52 | 9 | 60 | 8.27e-11 | 55.5 |
Find 13 sgRNAs with CRISPR-Local
Find 11 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCAAGAAACAATGCCTATA+GGG | 0.341997 | 8:+15179270 | None:intergenic |
| ATCCAAGAAACAATGCCTAT+AGG | 0.418860 | 8:+15179269 | None:intergenic |
| TTCACAAGCTCTTCATCTGT+TGG | 0.419604 | 8:-15179305 | None:intergenic |
| TCACAAGCTCTTCATCTGTT+GGG | 0.425279 | 8:-15179304 | None:intergenic |
| GGGCGAAATCTGTACCCTAT+AGG | 0.459914 | 8:-15179284 | None:intergenic |
| AGAGCTTGTGAAATTCTACT+TGG | 0.462581 | 8:+15179316 | MsG0880042874.01.T01:CDS |
| ATTCTACTTGGAACACAAGT+TGG | 0.488440 | 8:+15179328 | MsG0880042874.01.T01:CDS |
| ATAATACATGGTAATTTCCA+AGG | 0.536072 | 8:-15179407 | None:intergenic |
| AGATATAATATTATAATACA+TGG | 0.551825 | 8:-15179419 | None:intergenic |
| ACCTTGACATCATTCCACCT+TGG | 0.580282 | 8:+15179390 | MsG0880042874.01.T01:CDS |
| TACTTGGAACACAAGTTGGT+CGG | 0.595701 | 8:+15179332 | MsG0880042874.01.T01:CDS |
| TCCAAGGTGGAATGATGTCA+AGG | 0.605627 | 8:-15179391 | None:intergenic |
| ATACATGGTAATTTCCAAGG+TGG | 0.691316 | 8:-15179404 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGATATAATATTATAATACA+TGG | - | Chr8:15179422-15179441 | None:intergenic | 10.0% |
| ! | ATAATACATGGTAATTTCCA+AGG | - | Chr8:15179410-15179429 | None:intergenic | 25.0% |
| AGAGCTTGTGAAATTCTACT+TGG | + | Chr8:15179316-15179335 | MsG0880042874.01.T01:CDS | 35.0% | |
| ATACATGGTAATTTCCAAGG+TGG | - | Chr8:15179407-15179426 | None:intergenic | 35.0% | |
| ATTCTACTTGGAACACAAGT+TGG | + | Chr8:15179328-15179347 | MsG0880042874.01.T01:CDS | 35.0% | |
| TCACAAGCTCTTCATCTGTT+GGG | - | Chr8:15179307-15179326 | None:intergenic | 40.0% | |
| TTCACAAGCTCTTCATCTGT+TGG | - | Chr8:15179308-15179327 | None:intergenic | 40.0% | |
| !! | TACTTGGAACACAAGTTGGT+CGG | + | Chr8:15179332-15179351 | MsG0880042874.01.T01:CDS | 40.0% |
| ACCTTGACATCATTCCACCT+TGG | + | Chr8:15179390-15179409 | MsG0880042874.01.T01:CDS | 45.0% | |
| TCCAAGGTGGAATGATGTCA+AGG | - | Chr8:15179394-15179413 | None:intergenic | 45.0% | |
| GGGCGAAATCTGTACCCTAT+AGG | - | Chr8:15179287-15179306 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 15179281 | 15179463 | 15179281 | ID=MsG0880042874.01;Name=MsG0880042874.01 |
| Chr8 | mRNA | 15179281 | 15179463 | 15179281 | ID=MsG0880042874.01.T01;Parent=MsG0880042874.01;Name=MsG0880042874.01.T01;_AED=0.10;_eAED=0.10;_QI=0|-1|0|1|-1|0|1|0|60 |
| Chr8 | exon | 15179281 | 15179463 | 15179281 | ID=MsG0880042874.01.T01:exon:1002;Parent=MsG0880042874.01.T01 |
| Chr8 | CDS | 15179281 | 15179463 | 15179281 | ID=MsG0880042874.01.T01:cds;Parent=MsG0880042874.01.T01 |
| Gene Sequence |
| Protein sequence |
