Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043000.01.T01 | XP_003603053.1 | 93.407 | 273 | 15 | 3 | 289 | 560 | 1 | 271 | 2.15E-165 | 481 |
| MsG0880043000.01.T01 | XP_003603053.1 | 92.996 | 257 | 16 | 2 | 1 | 257 | 1 | 255 | 8.03E-155 | 454 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043000.01.T01 | MTR_3g101810 | 93.407 | 273 | 15 | 3 | 289 | 560 | 1 | 271 | 2.60e-169 | 481 |
| MsG0880043000.01.T01 | MTR_3g101810 | 92.996 | 257 | 16 | 2 | 1 | 257 | 1 | 255 | 9.73e-159 | 454 |
| MsG0880043000.01.T01 | MTR_5g017210 | 63.354 | 161 | 54 | 3 | 379 | 538 | 56 | 212 | 3.37e-46 | 162 |
| MsG0880043000.01.T01 | MTR_5g017210 | 61.491 | 161 | 57 | 3 | 91 | 250 | 56 | 212 | 1.38e-44 | 158 |
| MsG0880043000.01.T01 | MTR_8g093500 | 78.022 | 91 | 20 | 0 | 434 | 524 | 87 | 177 | 3.92e-44 | 158 |
| MsG0880043000.01.T01 | MTR_8g093500 | 77.778 | 90 | 20 | 0 | 146 | 235 | 87 | 176 | 1.31e-43 | 157 |
| MsG0880043000.01.T01 | MTR_2g100490 | 64.516 | 93 | 32 | 1 | 433 | 524 | 261 | 353 | 5.09e-26 | 110 |
| MsG0880043000.01.T01 | MTR_2g100490 | 62.366 | 93 | 34 | 1 | 145 | 236 | 261 | 353 | 3.93e-25 | 107 |
| MsG0880043000.01.T01 | MTR_8g015450 | 56.731 | 104 | 41 | 1 | 433 | 532 | 296 | 399 | 1.01e-25 | 110 |
| MsG0880043000.01.T01 | MTR_8g015450 | 55.340 | 103 | 42 | 1 | 145 | 243 | 296 | 398 | 1.17e-24 | 107 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043000.01.T01 | AT3G50330 | 80.000 | 110 | 21 | 1 | 434 | 542 | 92 | 201 | 5.95e-44 | 156 |
| MsG0880043000.01.T01 | AT3G50330 | 72.222 | 126 | 27 | 3 | 136 | 254 | 77 | 201 | 4.55e-43 | 154 |
| MsG0880043000.01.T01 | AT5G67060 | 88.298 | 94 | 11 | 0 | 434 | 527 | 83 | 176 | 1.35e-43 | 155 |
| MsG0880043000.01.T01 | AT5G67060 | 86.170 | 94 | 13 | 0 | 146 | 239 | 83 | 176 | 1.55e-42 | 152 |
| MsG0880043000.01.T01 | AT5G67060 | 88.298 | 94 | 11 | 0 | 434 | 527 | 95 | 188 | 1.76e-43 | 155 |
| MsG0880043000.01.T01 | AT5G67060 | 72.951 | 122 | 22 | 2 | 127 | 239 | 69 | 188 | 2.02e-42 | 152 |
| MsG0880043000.01.T01 | AT4G00120 | 70.787 | 89 | 26 | 0 | 436 | 524 | 87 | 175 | 1.91e-35 | 132 |
| MsG0880043000.01.T01 | AT4G00120 | 69.318 | 88 | 27 | 0 | 148 | 235 | 87 | 174 | 1.40e-34 | 129 |
| MsG0880043000.01.T01 | AT3G21330 | 66.304 | 92 | 31 | 0 | 433 | 524 | 241 | 332 | 9.77e-35 | 135 |
| MsG0880043000.01.T01 | AT3G21330 | 64.835 | 91 | 32 | 0 | 145 | 235 | 241 | 331 | 8.60e-34 | 132 |
Find 109 sgRNAs with CRISPR-Local
Find 232 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGGACAGTTGCTGGTTC+AGG | 0.344600 | 8:-17415826 | MsG0880043000.01.T01:CDS |
| AAACAAAAGATAAAGATGGA+GGG | 0.610409 | 8:+17416625 | None:intergenic |
| AAACTCGATGGCGGCGATGA+GGG | 0.479160 | 8:-17416130 | MsG0880043000.01.T01:CDS |
| AAACTCGATGGCGGCGATGA+GGG | 0.510188 | 8:-17419082 | MsG0880043000.01.T01:CDS |
| AAAGATAAAGATGGAGGGAA+AGG | 0.492583 | 8:+17416630 | None:intergenic |
| AAAGATGGACAAGTATTTAA+AGG | 0.256182 | 8:+17416657 | None:intergenic |
| AACAATAACAGAACAGTTGC+TGG | 0.438471 | 8:-17418784 | MsG0880043000.01.T01:CDS |
| AACAGAACAGTTGCTGGTTC+AGG | 0.322536 | 8:-17418778 | MsG0880043000.01.T01:CDS |
| AACAGTAAAAGGACAGTTGC+TGG | 0.446300 | 8:-17415832 | MsG0880043000.01.T01:CDS |
| AACCATGGTATTATTGGATT+AGG | 0.366504 | 8:+17416348 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACCATGGTATTATTGGATT+AGG | + | Chr8:17418927-17418946 | None:intergenic | 30.0% | |
| AATGATGAAGATGAAAACCA+TGG | + | Chr8:17418942-17418961 | None:intergenic | 30.0% | |
| ACAGGAATCGGTAAATAAAA+AGG | + | Chr8:17417967-17417986 | None:intergenic | 30.0% | |
| AGTAAGAAGAATGAATTCAC+AGG | + | Chr8:17417354-17417373 | None:intergenic | 30.0% | |
| ATCGTAATGTTGATCGTTAT+GGG | - | Chr8:17419507-17419526 | MsG0880043000.01.T01:CDS | 30.0% | |
| ATGATGAAGATGACTTTAAC+TGG | + | Chr8:17418728-17418747 | None:intergenic | 30.0% | |
| ATGCAAATGATTTGAATACG+TGG | + | Chr8:17418409-17418428 | None:intergenic | 30.0% | |
| CAGTTAGATGAAGAAAATTC+AGG | + | Chr8:17415846-17415865 | None:intergenic | 30.0% | |
| CGTTAGATGAAGAAAATTCA+GGG | + | Chr8:17418797-17418816 | None:intergenic | 30.0% | |
| CTCGAATTCGAAAACATTAA+CGG | + | Chr8:17419065-17419084 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 17415762 | 17419532 | 17415762 | ID=MsG0880043000.01;Name=MsG0880043000.01 |
| Chr8 | mRNA | 17415762 | 17419532 | 17415762 | ID=MsG0880043000.01.T01;Parent=MsG0880043000.01;Name=MsG0880043000.01.T01;_AED=0.29;_eAED=0.29;_QI=0|0|0|1|1|1|2|0|560 |
| Chr8 | exon | 17418772 | 17419532 | 17418772 | ID=MsG0880043000.01.T01:exon:3333;Parent=MsG0880043000.01.T01 |
| Chr8 | exon | 17415762 | 17416683 | 17415762 | ID=MsG0880043000.01.T01:exon:3332;Parent=MsG0880043000.01.T01 |
| Chr8 | CDS | 17418772 | 17419532 | 17418772 | ID=MsG0880043000.01.T01:cds;Parent=MsG0880043000.01.T01 |
| Chr8 | CDS | 17415762 | 17416683 | 17415762 | ID=MsG0880043000.01.T01:cds;Parent=MsG0880043000.01.T01 |
| Gene Sequence |
>MsG0880043000.01.T01 ATGGATTGTAACCAGTTAAAGTCATCTTCATTATCATCAGAGGATCAAATTGACATGATGTTAATGATGATGATGCAACTCCCTGAATTTTCTTCATCTAACTGTAATAATACCAACACCATCAATCAATTTCCACCGTCAAATCAACAATTCTATGCCACTTCAAATGTTTCTAACAATACATGTCCTCTTGCAGATTTAATAGATAACCCTCCTAATCCAATACCATGGTCTTCATCTTCATCATTCACTCATTTACCTTCACAATCCACCACAAACACAAACACAATCTCTTTAACCAATAACAACTCTACTACACCATTAATGTTCCAACAACAACAAACAACACCGTTAATGTTTTCTAATTCGAGCAACGAGATTGCTCCGAATACAAACCATTACGGTACTGCGTTGCCATCTGAGAAGAGAAACTCGATGGCGGCGATGAGGGAGATGATATTCAAAATAGCAGCTATGCAACCAATTTACATAGACCCAGAATCAGTAAAACCACCAAAGAGAAGAAACGTCAAAATTTCAAAAGATCCTCAGAGTATAGCAGCTAGACACAGAAGAGAAAGAATAAGCGAGAAAATAAGAATACTGCAAAGAATGGTACCAGGTGGAACTAAGATGGATACTGCATCAATGTTGGATGAAGCTATTCATTATGTTAAGTTTCTCAAGACACAGCTTAAGTCACTGCATGAACGTGCTTCAGGAGCCAACAATAACAGAACAGTTGCTGGTTCAGGATTAGGTTACCCTTTAAATACTTGTCCATCTTTTTCTCCTTTCCCTCCATCTTTATCTTTTGTTTCTAGTTTCACTTCTCTTCCTCTTAACCCTTCTTTGTCCCTCAAAATGGACTGTAACCAGTTAAAGTCATCTTCATCATCATCAGAGGATCAAATTGACATGATGTTAATGATGATGATGCAACTCCCTGAATTTTCTTCATCTAACGGTAATAACAACACCATCAATCAATTTCAACCGTCAGATCAACAATTCTATGGCACTTCAAATGCTTCTAACAATACACATCCTCTTGCAGATTTAATAGATAACCCTCCTAATCCAATAATACCATGGTTTTCATCTTCATCATTCATTCACTTACCCTCACAATCCATAACAAACACAAACACAATCTCTTTCACCAATAACAACTCTACTGCACCATTAAGGTTCCAACAACAACAAACAACACCGTTAATGTTTTCGAATTCGAGCAATGAGATTCCTCCGAATGCGAACCATTACGGTACTTCATCTCCATCAGAGAAGATAAACTCGATGGCGGCGATGAGGGAGATGATATTCAGAATAGCAGCGATGCAACCAATTTACATAGACCCAGAATCAGTAAAACCACCAAAGAGAAGAAACGTGAAGATTTCAAAAGATCCTCAGAGTGTTGCAGCTAGACACAGAAGAGAAAGAATAAGCGAGAAAATAAGAATACTGCAAAGAATGGTACCAGGTGGAACTAAGATGGATACTGCATCAATGTTGGATGAAGCTATTCATTATGTTAAGTTTCTCAAGACACAGCTTAAGTCACTGCAGGAACGTGCTTCAGGAGCCAACAGTAAAAGGACAGTTGCTGGTTCAGGATTAGGGTTCCCTGTTTCATTGCCTAAGCCTTACCAAGATCGTAATGTTGATCGTTATGGGTGA |
| Protein sequence |
>MsG0880043000.01.T01 MDCNQLKSSSLSSEDQIDMMLMMMMQLPEFSSSNCNNTNTINQFPPSNQQFYATSNVSNNTCPLADLIDNPPNPIPWSSSSSFTHLPSQSTTNTNTISLTNNNSTTPLMFQQQQTTPLMFSNSSNEIAPNTNHYGTALPSEKRNSMAAMREMIFKIAAMQPIYIDPESVKPPKRRNVKISKDPQSIAARHRRERISEKIRILQRMVPGGTKMDTASMLDEAIHYVKFLKTQLKSLHERASGANNNRTVAGSGLGYPLNTCPSFSPFPPSLSFVSSFTSLPLNPSLSLKMDCNQLKSSSSSSEDQIDMMLMMMMQLPEFSSSNGNNNTINQFQPSDQQFYGTSNASNNTHPLADLIDNPPNPIIPWFSSSSFIHLPSQSITNTNTISFTNNNSTAPLRFQQQQTTPLMFSNSSNEIPPNANHYGTSSPSEKINSMAAMREMIFRIAAMQPIYIDPESVKPPKRRNVKISKDPQSVAARHRRERISEKIRILQRMVPGGTKMDTASMLDEAIHYVKFLKTQLKSLQERASGANSKRTVAGSGLGFPVSLPKPYQDRNVDRYG* |
