AlfalfaGEDB | MsG0880043467.01

M. sativa cultivar ZhongmuNo.1 / MsG0880043467.01

KEGG: -
GO: GO:0000982 GO:0000987 GO:0003677 GO:0045944 GO:0046983
PFAM: PF00319

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880043467.01.T01	XP_013445020.1	90.964	166	13	1	1	164	1	166	3.84E-95	286

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880043467.01.T01	Q9FJK3	58.385	161	66	1	1	161	1	160	1.15E-58	188

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880043467.01.T01	A0A072TZT1	90.964	166	13	1	1	164	1	166	1.83e-95	286

TFs/TRs:

Gene ID	Type	Classification
MsG0880043467.01.T01	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880043467.01.T01	MTR_8g036130	90.964	166	13	1	1	164	1	166	4.65e-99	286
MsG0880043467.01.T01	MTR_4g032620	67.857	168	54	0	1	168	1	168	2.86e-81	240
MsG0880043467.01.T01	MTR_4g031910	70.732	164	48	0	1	164	1	164	1.90e-77	230
MsG0880043467.01.T01	MTR_3g466980	67.742	155	49	1	1	155	1	154	6.52e-76	226
MsG0880043467.01.T01	MTR_3g065100	69.375	160	48	1	1	160	1	159	4.57e-73	219
MsG0880043467.01.T01	MTR_4g032290	70.732	164	48	0	1	164	1	164	8.71e-72	216
MsG0880043467.01.T01	MTR_4g032260	70.513	156	46	0	1	156	1	156	5.39e-69	206
MsG0880043467.01.T01	MTR_2g035610	77.049	122	28	0	1	122	1	122	1.73e-62	189
MsG0880043467.01.T01	MTR_3g466830	50.314	159	78	1	3	161	2	159	1.38e-55	172
MsG0880043467.01.T01	MTR_4g063790	47.403	154	81	0	1	154	1	154	5.51e-50	159
MsG0880043467.01.T01	MTR_3g466890	46.203	158	84	1	3	160	2	158	5.19e-48	153
MsG0880043467.01.T01	MTR_3g467080	43.312	157	89	0	1	157	1	157	7.93e-47	150
MsG0880043467.01.T01	MTR_2g016210	44.970	169	89	1	1	169	1	165	9.28e-47	153
MsG0880043467.01.T01	MTR_5g047580	45.860	157	83	2	1	157	1	155	2.00e-45	146
MsG0880043467.01.T01	MTR_3g031100	42.675	157	90	0	1	157	1	157	1.44e-43	144
MsG0880043467.01.T01	MTR_5g075380	40.909	154	90	1	1	154	1	153	8.79e-43	140
MsG0880043467.01.T01	MTR_3g031240	39.506	162	98	0	1	162	1	162	8.08e-40	133
MsG0880043467.01.T01	MTR_1g090783	40.385	156	91	2	3	158	2	155	4.91e-38	128
MsG0880043467.01.T01	MTR_7g011950	44.586	157	85	2	1	157	1	155	1.04e-37	127
MsG0880043467.01.T01	MTR_1g090697	37.975	158	96	2	1	158	1	156	3.87e-37	125
MsG0880043467.01.T01	MTR_1g084950	43.949	157	86	2	1	157	1	155	4.32e-37	125
MsG0880043467.01.T01	MTR_1g077390	39.490	157	93	2	1	157	1	155	9.01e-37	124
MsG0880043467.01.T01	MTR_1g090710	36.709	158	99	1	1	158	1	157	1.50e-36	124
MsG0880043467.01.T01	MTR_7g055800	56.303	119	24	2	1	119	1	91	2.30e-35	119
MsG0880043467.01.T01	MTR_1g077320	42.675	157	88	2	1	157	1	155	4.81e-35	120
MsG0880043467.01.T01	MTR_3g466900	41.085	129	75	1	32	160	2	129	7.68e-35	119
MsG0880043467.01.T01	MTR_4g028720	42.675	157	88	2	1	157	1	155	8.01e-35	120
MsG0880043467.01.T01	MTR_3g465410	42.857	126	72	0	29	154	1	126	8.24e-34	117
MsG0880043467.01.T01	MTR_3g466930	41.860	129	74	1	32	160	2	129	9.77e-34	116
MsG0880043467.01.T01	MTR_1g077300	43.949	157	84	2	1	157	1	153	6.82e-33	115
MsG0880043467.01.T01	MTR_4g028800	73.611	72	17	1	1	72	1	70	8.34e-33	112
MsG0880043467.01.T01	MTR_2g035580	41.667	156	90	1	1	156	1	155	1.24e-32	114
MsG0880043467.01.T01	MTR_7g055790	55.856	111	31	1	1	111	59	151	3.99e-31	110
MsG0880043467.01.T01	MTR_7g055940	55.660	106	19	2	1	106	1	78	2.33e-29	103
MsG0880043467.01.T01	MTR_2g035590	63.333	90	33	0	74	163	1	90	7.61e-28	100
MsG0880043467.01.T01	MTR_5g047560	36.306	157	82	3	1	157	1	139	9.15e-27	99.0
MsG0880043467.01.T01	MTR_4g019670	41.406	128	72	1	1	125	1	128	2.04e-26	104
MsG0880043467.01.T01	MTR_7g106510	38.298	141	78	2	1	138	1	135	1.20e-25	102
MsG0880043467.01.T01	MTR_3g093900	33.103	145	97	0	1	145	4	148	4.60e-24	98.2
MsG0880043467.01.T01	MTR_4g084780	69.231	52	16	0	74	125	1	52	8.57e-19	78.6
MsG0880043467.01.T01	MTR_6g018920	27.607	163	103	6	1	157	1	154	6.72e-14	68.6
MsG0880043467.01.T01	MTR_1g114730	28.319	113	74	3	1	107	1	112	2.53e-12	63.9
MsG0880043467.01.T01	MTR_6g005450	31.858	113	70	4	1	107	1	112	6.42e-12	62.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880043467.01.T01	AT5G48670	58.385	161	66	1	1	161	1	160	1.17e-59	188
MsG0880043467.01.T01	AT1G65300	44.172	163	90	1	3	165	2	163	1.35e-44	148
MsG0880043467.01.T01	AT3G05860	44.512	164	90	1	1	164	1	163	5.88e-43	142
MsG0880043467.01.T01	AT5G26650	43.396	159	89	1	4	162	2	159	1.48e-42	145
MsG0880043467.01.T01	AT3G05860	44.512	164	90	1	1	164	1	163	3.23e-42	141
MsG0880043467.01.T01	AT5G26630	48.485	165	84	1	1	165	1	164	5.11e-42	140
MsG0880043467.01.T01	AT3G05860	44.512	164	90	1	1	164	1	163	5.33e-42	141
MsG0880043467.01.T01	AT5G27960	42.767	159	90	1	4	162	2	159	8.52e-42	142
MsG0880043467.01.T01	AT1G65330	42.331	163	93	1	3	165	2	163	1.27e-40	138
MsG0880043467.01.T01	AT5G27810	59.140	93	38	0	29	121	1	93	3.68e-38	127
MsG0880043467.01.T01	AT1G31630	36.810	163	101	2	3	165	2	162	9.79e-37	129
MsG0880043467.01.T01	AT2G28700	36.145	166	101	2	1	164	1	163	1.83e-34	123
MsG0880043467.01.T01	AT1G31640	37.423	163	100	2	3	165	2	162	3.45e-34	125
MsG0880043467.01.T01	AT1G22590	33.742	163	104	1	1	163	1	159	3.86e-30	108
MsG0880043467.01.T01	AT5G26580	36.646	161	87	2	1	161	1	146	3.73e-29	110
MsG0880043467.01.T01	AT2G40210	40.152	132	79	0	1	132	1	132	3.44e-22	91.7
MsG0880043467.01.T01	AT5G06500	29.870	154	106	1	1	154	1	152	3.53e-20	84.3
MsG0880043467.01.T01	AT5G58890	41.964	112	56	4	1	105	1	110	7.53e-16	73.6
MsG0880043467.01.T01	AT5G55690	35.484	124	71	3	1	115	1	124	5.91e-15	70.9
MsG0880043467.01.T01	AT5G55690	35.484	124	71	3	1	115	1	124	5.91e-15	70.9
MsG0880043467.01.T01	AT2G15660	26.829	123	88	2	38	160	31	151	5.84e-13	65.1

Find 38 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCTTGCTTTGTTCCATTTC+AGG	0.136228	8:-25259047	None:intergenic
TCCCAATGTCCTTCAAATTT+TGG	0.150964	8:-25259256	None:intergenic
GAAGCTTGTGCTATAATTTA+CGG	0.231910	8:+25258948	MsG0880043467.01.T01:CDS
GGACTAATCATGATTGATTC+TGG	0.252810	8:-25259319	None:intergenic
TCAGACATTGAAACATTAGA+AGG	0.343484	8:-25259166	None:intergenic
ATGAACCACAACCTGAGATT+TGG	0.352882	8:+25258976	MsG0880043467.01.T01:CDS
CTTTAGCCTTCATCACCCTT+TGG	0.360586	8:-25259097	None:intergenic
ACCAAAATTTGAAGGACATT+GGG	0.367874	8:+25259255	MsG0880043467.01.T01:CDS
ATTCAAGACAATGCCTGAAA+TGG	0.391921	8:+25259034	MsG0880043467.01.T01:CDS
TGAGATTTGGCCATCCCCTT+GGG	0.393251	8:+25258989	MsG0880043467.01.T01:CDS
ATCTCAGGTTGTGGTTCATA+AGG	0.415912	8:-25258972	None:intergenic
GACCAAAATTTGAAGGACAT+TGG	0.425243	8:+25259254	MsG0880043467.01.T01:CDS
GGAAAGCAACATACAAGAAA+AGG	0.431751	8:+25258874	MsG0880043467.01.T01:CDS
GTTGATTGACCAAAATTTGA+AGG	0.435105	8:+25259247	MsG0880043467.01.T01:CDS
GACGAACTAAGCACACTTTG+TGG	0.444181	8:+25258921	MsG0880043467.01.T01:CDS
CAAGGGGATGGCCAAATCTC+AGG	0.453389	8:-25258987	None:intergenic
CTGAGATTTGGCCATCCCCT+TGG	0.462588	8:+25258988	MsG0880043467.01.T01:CDS
TTTCACCTTTCTTCTAGTCA+TGG	0.469195	8:-25258823	None:intergenic
GAAGGACATTGGGAGAAGGT+TGG	0.475154	8:+25259265	MsG0880043467.01.T01:CDS
TTGGAAGCAGTGGACAATAA+TGG	0.490759	8:+25259284	MsG0880043467.01.T01:CDS
AACATACAAGAAAAGGAAGA+AGG	0.492306	8:+25258881	MsG0880043467.01.T01:CDS
ATGTTTCAATGTCTGAATGC+AGG	0.499753	8:+25259173	MsG0880043467.01.T01:CDS
TTGATGTGAATCATCTTACT+TGG	0.506323	8:+25259225	MsG0880043467.01.T01:CDS
CATGAACCAAAGGGTGATGA+AGG	0.509359	8:+25259091	MsG0880043467.01.T01:CDS
TGGGAGAAGGTTGGAAGCAG+TGG	0.521549	8:+25259274	MsG0880043467.01.T01:CDS
CAAAACCATGACTAGAAGAA+AGG	0.529842	8:+25258818	None:intergenic
TGTTTCAATGTCTGAATGCA+GGG	0.544428	8:+25259174	MsG0880043467.01.T01:CDS
ATTTGAAGGACATTGGGAGA+AGG	0.551499	8:+25259261	MsG0880043467.01.T01:CDS
TGCAGCGTCATTGACAATGA+AGG	0.552900	8:-25258850	None:intergenic
AAGAGACTTTCATGAACCAA+AGG	0.569196	8:+25259081	MsG0880043467.01.T01:CDS
GATGGCCAAATCTCAGGTTG+TGG	0.572435	8:-25258981	None:intergenic
GGTGATGAAGGCTAAAGAGC+AGG	0.574380	8:+25259103	MsG0880043467.01.T01:CDS
GAGATTTGGCCATCCCCTTG+GGG	0.591825	8:+25258990	MsG0880043467.01.T01:CDS
AGAGACTTTCATGAACCAAA+GGG	0.592104	8:+25259082	MsG0880043467.01.T01:CDS
ACATACAAGAAAAGGAAGAA+GGG	0.593121	8:+25258882	MsG0880043467.01.T01:CDS
TCATTGTCAATGACGCTGCA+AGG	0.601167	8:+25258853	MsG0880043467.01.T01:CDS
CAGACATTGAAACATTAGAA+GGG	0.603894	8:-25259165	None:intergenic
TGTGCAGAACAATATGTCAG+TGG	0.716818	8:+25259202	MsG0880043467.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAAACAACAAAAAGACAACA+AGG	+	Chr8:25259130-25259149	MsG0880043467.01.T01:CDS	25.0%
	AACATACAAGAAAAGGAAGA+AGG	+	Chr8:25258881-25258900	MsG0880043467.01.T01:CDS	30.0%
	ACATACAAGAAAAGGAAGAA+GGG	+	Chr8:25258882-25258901	MsG0880043467.01.T01:CDS	30.0%
	ACCAAAATTTGAAGGACATT+GGG	+	Chr8:25259255-25259274	MsG0880043467.01.T01:CDS	30.0%
	CAGACATTGAAACATTAGAA+GGG	-	Chr8:25259168-25259187	None:intergenic	30.0%
	GAAGCTTGTGCTATAATTTA+CGG	+	Chr8:25258948-25258967	MsG0880043467.01.T01:CDS	30.0%
	GTTGATTGACCAAAATTTGA+AGG	+	Chr8:25259247-25259266	MsG0880043467.01.T01:CDS	30.0%
	TCAGACATTGAAACATTAGA+AGG	-	Chr8:25259169-25259188	None:intergenic	30.0%
	TTGATGTGAATCATCTTACT+TGG	+	Chr8:25259225-25259244	MsG0880043467.01.T01:CDS	30.0%
!!	TGAATTTTGAGAGCACATTT+TGG	-	Chr8:25259019-25259038	None:intergenic	30.0%
	AAGAGACTTTCATGAACCAA+AGG	+	Chr8:25259081-25259100	MsG0880043467.01.T01:CDS	35.0%
	AGAGACTTTCATGAACCAAA+GGG	+	Chr8:25259082-25259101	MsG0880043467.01.T01:CDS	35.0%
	ATGTTTCAATGTCTGAATGC+AGG	+	Chr8:25259173-25259192	MsG0880043467.01.T01:CDS	35.0%
	ATTCAAGACAATGCCTGAAA+TGG	+	Chr8:25259034-25259053	MsG0880043467.01.T01:CDS	35.0%
	GACCAAAATTTGAAGGACAT+TGG	+	Chr8:25259254-25259273	MsG0880043467.01.T01:CDS	35.0%
	GGAAAGCAACATACAAGAAA+AGG	+	Chr8:25258874-25258893	MsG0880043467.01.T01:CDS	35.0%
	TGTTTCAATGTCTGAATGCA+GGG	+	Chr8:25259174-25259193	MsG0880043467.01.T01:CDS	35.0%
!	TCCCAATGTCCTTCAAATTT+TGG	-	Chr8:25259259-25259278	None:intergenic	35.0%
!	TTCTTGCTTTGTTCCATTTC+AGG	-	Chr8:25259050-25259069	None:intergenic	35.0%
	ATGAACCACAACCTGAGATT+TGG	+	Chr8:25258976-25258995	MsG0880043467.01.T01:CDS	40.0%
	ATTTGAAGGACATTGGGAGA+AGG	+	Chr8:25259261-25259280	MsG0880043467.01.T01:CDS	40.0%
	TGTGCAGAACAATATGTCAG+TGG	+	Chr8:25259202-25259221	MsG0880043467.01.T01:CDS	40.0%
	TTGGAAGCAGTGGACAATAA+TGG	+	Chr8:25259284-25259303	MsG0880043467.01.T01:CDS	40.0%
!	ATCTCAGGTTGTGGTTCATA+AGG	-	Chr8:25258975-25258994	None:intergenic	40.0%
	CATGAACCAAAGGGTGATGA+AGG	+	Chr8:25259091-25259110	MsG0880043467.01.T01:CDS	45.0%
	GACGAACTAAGCACACTTTG+TGG	+	Chr8:25258921-25258940	MsG0880043467.01.T01:CDS	45.0%
	TCATTGTCAATGACGCTGCA+AGG	+	Chr8:25258853-25258872	MsG0880043467.01.T01:CDS	45.0%
	TGCAGCGTCATTGACAATGA+AGG	-	Chr8:25258853-25258872	None:intergenic	45.0%
!	CTTTAGCCTTCATCACCCTT+TGG	-	Chr8:25259100-25259119	None:intergenic	45.0%
	GAAGGACATTGGGAGAAGGT+TGG	+	Chr8:25259265-25259284	MsG0880043467.01.T01:CDS	50.0%
	GGTGATGAAGGCTAAAGAGC+AGG	+	Chr8:25259103-25259122	MsG0880043467.01.T01:CDS	50.0%
	TGAGATTTGGCCATCCCCTT+GGG	+	Chr8:25258989-25259008	MsG0880043467.01.T01:CDS	50.0%
!	AGCACATTTTGGACTCCCCA+AGG	-	Chr8:25259008-25259027	None:intergenic	50.0%
!	CACATTTTGGACTCCCCAAG+GGG	-	Chr8:25259006-25259025	None:intergenic	50.0%
!	GATGGCCAAATCTCAGGTTG+TGG	-	Chr8:25258984-25259003	None:intergenic	50.0%
!	GCACATTTTGGACTCCCCAA+GGG	-	Chr8:25259007-25259026	None:intergenic	50.0%
	CAAGGGGATGGCCAAATCTC+AGG	-	Chr8:25258990-25259009	None:intergenic	55.0%
	CTGAGATTTGGCCATCCCCT+TGG	+	Chr8:25258988-25259007	MsG0880043467.01.T01:CDS	55.0%
	GAGATTTGGCCATCCCCTTG+GGG	+	Chr8:25258990-25259009	MsG0880043467.01.T01:CDS	55.0%
	TGGGAGAAGGTTGGAAGCAG+TGG	+	Chr8:25259274-25259293	MsG0880043467.01.T01:CDS	55.0%
!	TTTTGGACTCCCCAAGGGGA+TGG	-	Chr8:25259002-25259021	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	25258825	25259334	25258825	ID=MsG0880043467.01;Name=MsG0880043467.01
Chr8	mRNA	25258825	25259334	25258825	ID=MsG0880043467.01.T01;Parent=MsG0880043467.01;Name=MsG0880043467.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|169
Chr8	exon	25258825	25259334	25258825	ID=MsG0880043467.01.T01:exon:14165;Parent=MsG0880043467.01.T01
Chr8	CDS	25258825	25259334	25258825	ID=MsG0880043467.01.T01:cds;Parent=MsG0880043467.01.T01

Gene Sequence

>MsG0880043467.01.T01

ATGACTAGAAGAAAGGTGAAACTTGCCTTCATTGTCAATGACGCTGCAAGGAAAGCAACATACAAGAAAAGGAAGAAGGGTTTATTGAAGAAGATCGACGAACTAAGCACACTTTGTGGAATTGAAGCTTGTGCTATAATTTACGGTCCTTATGAACCACAACCTGAGATTTGGCCATCCCCTTGGGGAGTCCAAAATGTGCTCTCAAAATTCAAGACAATGCCTGAAATGGAACAAAGCAAGAAAATGATGAATCAAGAGACTTTCATGAACCAAAGGGTGATGAAGGCTAAAGAGCAGGTGAAAAAACAACAAAAAGACAACAAGGAGAAAGAGATGACCCTTCTAATGTTTCAATGTCTGAATGCAGGGAAAATTGTGCAGAACAATATGTCAGTGGTTGATGTGAATCATCTTACTTGGTTGATTGACCAAAATTTGAAGGACATTGGGAGAAGGTTGGAAGCAGTGGACAATAATGGTCAAATTCAAAGCCAGAATCAATCATGA

Protein sequence

>MsG0880043467.01.T01

MTRRKVKLAFIVNDAARKATYKKRKKGLLKKIDELSTLCGIEACAIIYGPYEPQPEIWPSPWGVQNVLSKFKTMPEMEQSKKMMNQETFMNQRVMKAKEQVKKQQKDNKEKEMTLLMFQCLNAGKIVQNNMSVVDVNHLTWLIDQNLKDIGRRLEAVDNNGQIQSQNQS*