AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043643.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043643.01.T01 MTR_8g039110 95.614 114 5 0 1 114 164 277 1.98e-66 206
MsG0880043643.01.T01 MTR_3g014660 70.330 91 22 3 24 114 196 281 4.79e-35 125
MsG0880043643.01.T01 MTR_5g089750 64.407 59 20 1 56 114 339 396 1.44e-18 82.0
MsG0880043643.01.T01 MTR_0363s0040 90.566 53 5 0 1 53 164 216 3.07e-16 73.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043643.01.T01 AT5G12330 45.000 100 51 1 24 123 178 273 6.12e-17 76.6
MsG0880043643.01.T01 AT5G12330 45.000 100 51 1 24 123 178 273 6.12e-17 76.6
MsG0880043643.01.T01 AT5G12330 45.000 100 51 1 24 123 265 360 8.21e-17 77.0
MsG0880043643.01.T01 AT3G54430 53.226 62 28 1 54 114 104 165 1.79e-13 65.5

Find 79 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTTGTTGAGATTGCTTTA+AGG 0.163317 8:-28298799 MsG0880043643.01.T01:CDS
TCATTGTAATTGTTATTGTT+AGG 0.303182 8:+28300125 None:intergenic
GAGAATAGAATAGGGAGATT+TGG 0.314018 8:+28300484 None:intergenic
GATGAAATGGTTTGGTTTGT+TGG 0.314201 8:+28300248 None:intergenic
CATTGTAATTGTTATTGTTA+GGG 0.340659 8:+28300126 None:intergenic
CTATGAGAACCAAGTCTCTT+AGG 0.342592 8:+28300296 None:intergenic
TGAAGATGAACTTGCTTACT+TGG 0.368954 8:-28298999 MsG0880043643.01.T01:CDS
AGTGATGGTGGTGCTTCTTC+TGG 0.380721 8:-28299775 MsG0880043643.01.T01:CDS
GTAAAGAGAGGATGAGGTTA+AGG 0.383053 8:+28300385 None:intergenic
GATGAAGGTAAGGGAATGTT+TGG 0.393591 8:+28300215 None:intergenic
AGTAGCGTTGAGAATAGAAT+AGG 0.393972 8:+28300475 None:intergenic
GTGAGGAAAATGGTGAGTTT+AGG 0.401694 8:-28299961 MsG0880043643.01.T01:five_prime_UTR
TGCCAATAGCAGATACTCTA+TGG 0.417109 8:+28299026 None:intergenic
GATGATGGGTGTGAGGAAAA+TGG 0.420089 8:-28299971 MsG0880043643.01.T01:five_prime_UTR
ATGTTCTTCTTCTGCAATTG+AGG 0.433224 8:-28298847 MsG0880043643.01.T01:CDS
GTAGCGTTGAGAATAGAATA+GGG 0.438938 8:+28300476 None:intergenic
TGCCATAGAGTATCTGCTAT+TGG 0.440334 8:-28299028 MsG0880043643.01.T01:CDS
TGGATTATGATCTGATGAAA+TGG 0.452099 8:+28300235 None:intergenic
CAAAGGTTTCTCTATGATCA+AGG 0.455592 8:-28298948 MsG0880043643.01.T01:CDS
TATGATCTGATGAAATGGTT+TGG 0.460879 8:+28300240 None:intergenic
GAAACAAAACTTGATGATGA+TGG 0.462474 8:-28299986 MsG0880043643.01.T01:five_prime_UTR
GATGTAGTGTGAGTGTGATT+TGG 0.468027 8:+28300429 None:intergenic
GAATTGCAACTTGGAAGTAA+TGG 0.469782 8:-28298888 MsG0880043643.01.T01:CDS
CAATCTCAACAAGCAGAGGC+AGG 0.472994 8:+28298807 None:intergenic
TAGAGCTGATGATGAAGGTA+AGG 0.474808 8:+28300205 None:intergenic
CAGAAAGGCACCACTGCTAC+AGG 0.479617 8:+28300509 None:intergenic
TGTTGGTTGTGAAGTGATGA+AGG 0.480742 8:+28300265 None:intergenic
TCATCATTACTTGTTGTGGT+TGG 0.483361 8:+28300060 None:intergenic
TCAGCTCTAAGTGTTGGTCT+TGG 0.484915 8:-28300191 MsG0880043643.01.T01:five_prime_UTR
GTTTAGGTTTGTCAAGATTG+TGG 0.485617 8:-28299945 MsG0880043643.01.T01:five_prime_UTR
AGATGTAGGACTTGCTGTAA+AGG 0.491124 8:-28299892 MsG0880043643.01.T01:five_prime_UTR
CATGTTAGGCCTAAGAGACT+TGG 0.493330 8:-28300305 MsG0880043643.01.T01:five_prime_UTR
TTCATCATCATTACTTGTTG+TGG 0.498315 8:+28300056 None:intergenic
AATCAAAATTCTCTGCCTCT+TGG 0.499143 8:+28299750 None:intergenic
CGTGAAGAGCACGTGGATAC+CGG 0.503179 8:-28299845 MsG0880043643.01.T01:five_prime_UTR
CCAAAGAAAGAATATGTAAG+TGG 0.507300 8:-28300554 None:intergenic
TGTAATGTAAAGAGAGGATG+AGG 0.514738 8:+28300379 None:intergenic
CAACTTGGAAGTAATGGAAG+TGG 0.519956 8:-28298882 MsG0880043643.01.T01:CDS
GGCAAAGTCTGCTTGAAACC+AGG 0.531084 8:+28299082 None:intergenic
GATTGTAGCTATAAAGATGT+AGG 0.536651 8:-28299906 MsG0880043643.01.T01:five_prime_UTR
TAGAGAAACCTTTGAATACA+TGG 0.543808 8:+28298957 None:intergenic
TTGGCCACTGTGAATATAAG+TGG 0.544977 8:-28298980 MsG0880043643.01.T01:CDS
GCAGGCGCGCGAACATAGCG+AGG 0.545668 8:+28299061 None:intergenic
ATAAGTGGCCATGTATTCAA+AGG 0.555059 8:-28298965 MsG0880043643.01.T01:CDS
CTATGGCACTTGAAAACAGC+AGG 0.559201 8:+28299043 None:intergenic
CGGTCCCGTCTCATCGAAGC+CGG 0.561361 8:+28299826 None:intergenic
GGGTGAGTGGTGGTTAGTAG+TGG 0.566513 8:+28300161 None:intergenic
AGAGTATCTGCTATTGGCAA+TGG 0.570693 8:-28299022 MsG0880043643.01.T01:CDS
GCTTAAACTTCTAGTTCTAG+TGG 0.572204 8:+28299672 None:intergenic
GGAGAATGTAATGTAAAGAG+AGG 0.573573 8:+28300373 None:intergenic
AGGACTTGCTGTAAAGGTCG+TGG 0.574448 8:-28299886 MsG0880043643.01.T01:five_prime_UTR
GAGACGGGACCGGAGTATGA+TGG 0.578242 8:-28299815 MsG0880043643.01.T01:CDS
ACACTTAGAGCTGATGATGA+AGG 0.580452 8:+28300200 None:intergenic
TGTGTTTCAGAATTGCAACT+TGG 0.580875 8:-28298897 MsG0880043643.01.T01:CDS
GGATACCGGCTTCGATGAGA+CGG 0.585711 8:-28299831 MsG0880043643.01.T01:exon
CGGAGTATGATGGTGGCTGA+AGG 0.587151 8:-28299805 MsG0880043643.01.T01:CDS
GCTGAAGGTGGTGACAGTGA+TGG 0.591111 8:-28299790 MsG0880043643.01.T01:CDS
AAACAAAACTTGATGATGAT+GGG 0.592077 8:-28299985 MsG0880043643.01.T01:five_prime_UTR
TCATCATCAGCTCTAAGTGT+TGG 0.595371 8:-28300197 MsG0880043643.01.T01:five_prime_UTR
GAAGGTGGTGACAGTGATGG+TGG 0.597594 8:-28299787 MsG0880043643.01.T01:CDS
TTGATGATGAGGGTGAGTGG+TGG 0.600442 8:+28300151 None:intergenic
TAGGGAGATTTGGAACAGAA+AGG 0.606424 8:+28300494 None:intergenic
CGGCTTCGATGAGACGGGAC+CGG 0.606559 8:-28299825 MsG0880043643.01.T01:exon
AGAGCTGATGATGAAGGTAA+GGG 0.617936 8:+28300206 None:intergenic
GATACCGGCTTCGATGAGAC+GGG 0.619399 8:-28299830 MsG0880043643.01.T01:exon
AATCTCAACAAGCAGAGGCA+GGG 0.619976 8:+28298808 None:intergenic
GTTGTGGTTGGCACATCTTG+AGG 0.623886 8:+28300072 None:intergenic
GGTGAGTGGTGGTTAGTAGT+GGG 0.627587 8:+28300162 None:intergenic
AAAGCAATCTCAACAAGCAG+AGG 0.639282 8:+28298803 None:intergenic
AGAATTCAAACCTGTAGCAG+TGG 0.640764 8:-28300519 MsG0880043643.01.T01:five_prime_UTR
GTGCTTCTTCTGGTACCAAG+AGG 0.651535 8:-28299765 MsG0880043643.01.T01:CDS
CTTCAGCCACCATCATACTC+CGG 0.656848 8:+28299806 None:intergenic
AGTATGATGGTGGCTGAAGG+TGG 0.658861 8:-28299802 MsG0880043643.01.T01:CDS
ACTTGATGATGATGGGTGTG+AGG 0.668195 8:-28299978 MsG0880043643.01.T01:five_prime_UTR
ATAACTAGCTCTTGTCACCA+AGG 0.669802 8:-28299643 MsG0880043643.01.T01:intron
GTACTCACGTGAAGAGCACG+TGG 0.684544 8:-28299852 MsG0880043643.01.T01:five_prime_UTR
AGTTGCAATTCTGAAACACA+AGG 0.685469 8:+28298900 None:intergenic
ACGGGACCGGAGTATGATGG+TGG 0.723586 8:-28299812 MsG0880043643.01.T01:CDS
ATGGCCACTTATATTCACAG+TGG 0.791885 8:+28298976 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATGATTTATTTTAATCA+TGG + Chr8:28299737-28299756 None:intergenic 10.0%
!!! TTGTTATTTCATAAATTTAA+GGG - Chr8:28299994-28300013 MsG0880043643.01.T01:five_prime_UTR 10.0%
!!! TTTGTTATTTCATAAATTTA+AGG - Chr8:28299993-28300012 MsG0880043643.01.T01:five_prime_UTR 10.0%
!! ATTAAAAAAAGTGAGTAAAT+AGG + Chr8:28300063-28300082 None:intergenic 15.0%
!!! GTTTACAAAACTTTTTTTTA+TGG - Chr8:28299765-28299784 MsG0880043643.01.T01:CDS 15.0%
!!! TTTTGGTTTTGTTATTTATT+GGG + Chr8:28298988-28299007 None:intergenic 15.0%
!!! CATTGTAATTGTTATTGTTA+GGG + Chr8:28299214-28299233 None:intergenic 20.0%
!!! GTTTTGGTTTTGTTATTTAT+TGG + Chr8:28298989-28299008 None:intergenic 20.0%
!!! TCATTGTAATTGTTATTGTT+AGG + Chr8:28299215-28299234 None:intergenic 20.0%
!!! TCATTGTTACTTTATAAACT+AGG - Chr8:28300086-28300105 MsG0880043643.01.T01:five_prime_UTR 20.0%
!!! TTTTTCTATTAACCAAACTT+AGG + Chr8:28300142-28300161 None:intergenic 20.0%
! AATTGTGTATATCCTAAGTT+TGG - Chr8:28300127-28300146 MsG0880043643.01.T01:five_prime_UTR 25.0%
! CGGATAATGATTTACAAAAA+TGG - Chr8:28299854-28299873 MsG0880043643.01.T01:five_prime_UTR 25.0%
!!! AAACAAAACTTGATGATGAT+GGG - Chr8:28299352-28299371 MsG0880043643.01.T01:intron 25.0%
!!! GTTATGTGTTGTTTTCTTTA+GGG + Chr8:28298889-28298908 None:intergenic 25.0%
!!! TTATGTGTTGTTTTCTTTAG+GGG + Chr8:28298888-28298907 None:intergenic 25.0%
ATCAATCAATTTAGTCTCAG+AGG + Chr8:28299953-28299972 None:intergenic 30.0%
GATTGTAGCTATAAAGATGT+AGG - Chr8:28299431-28299450 MsG0880043643.01.T01:intron 30.0%
GTTGCAAAAATGAACATGTT+AGG - Chr8:28299018-28299037 MsG0880043643.01.T01:CDS 30.0%
TAGAGAAACCTTTGAATACA+TGG + Chr8:28300383-28300402 None:intergenic 30.0%
TATGATCTGATGAAATGGTT+TGG + Chr8:28299100-28299119 None:intergenic 30.0%
TGGATTATGATCTGATGAAA+TGG + Chr8:28299105-28299124 None:intergenic 30.0%
TTGTAAATCATTATCCGTGT+CGG + Chr8:28299851-28299870 None:intergenic 30.0%
! TTCATCAAGACTCAAGTTTT+TGG - Chr8:28299240-28299259 MsG0880043643.01.T01:intron 30.0%
! TTCATCATCATTACTTGTTG+TGG + Chr8:28299284-28299303 None:intergenic 30.0%
!! AACAAAAAGTGAAAGTACCT+TGG + Chr8:28299714-28299733 None:intergenic 30.0%
!! ACATGTTCATTTTTGCAACA+AGG + Chr8:28299017-28299036 None:intergenic 30.0%
!! GAAACAAAACTTGATGATGA+TGG - Chr8:28299351-28299370 MsG0880043643.01.T01:intron 30.0%
!! TCATTTTTGCAACAAGGTTT+TGG + Chr8:28299011-28299030 None:intergenic 30.0%
!!! GGTTATGTGTTGTTTTCTTT+AGG + Chr8:28298890-28298909 None:intergenic 30.0%
AATCAAAATTCTCTGCCTCT+TGG + Chr8:28299590-28299609 None:intergenic 35.0%
ATAAGTGGCCATGTATTCAA+AGG - Chr8:28300372-28300391 MsG0880043643.01.T01:five_prime_UTR 35.0%
ATGTTCTTCTTCTGCAATTG+AGG - Chr8:28300490-28300509 MsG0880043643.01.T01:five_prime_UTR 35.0%
CAAAGGTTTCTCTATGATCA+AGG - Chr8:28300389-28300408 MsG0880043643.01.T01:five_prime_UTR 35.0%
GAATTGCAACTTGGAAGTAA+TGG - Chr8:28300449-28300468 MsG0880043643.01.T01:five_prime_UTR 35.0%
GAGAATAGAATAGGGAGATT+TGG + Chr8:28298856-28298875 None:intergenic 35.0%
GCATAATCCAGTGTAATCAT+AGG - Chr8:28299876-28299895 MsG0880043643.01.T01:five_prime_UTR 35.0%
GGAGAATGTAATGTAAAGAG+AGG + Chr8:28298967-28298986 None:intergenic 35.0%
TGTAATGTAAAGAGAGGATG+AGG + Chr8:28298961-28298980 None:intergenic 35.0%
TGTGTTTCAGAATTGCAACT+TGG - Chr8:28300440-28300459 MsG0880043643.01.T01:five_prime_UTR 35.0%
! AGTAGCGTTGAGAATAGAAT+AGG + Chr8:28298865-28298884 None:intergenic 35.0%
! GTAGCGTTGAGAATAGAATA+GGG + Chr8:28298864-28298883 None:intergenic 35.0%
! GTTTAGGTTTGTCAAGATTG+TGG - Chr8:28299392-28299411 MsG0880043643.01.T01:intron 35.0%
! TGAAGATGAACTTGCTTACT+TGG - Chr8:28300338-28300357 MsG0880043643.01.T01:five_prime_UTR 35.0%
! TGCTTGTTGAGATTGCTTTA+AGG - Chr8:28300538-28300557 MsG0880043643.01.T01:five_prime_UTR 35.0%
!! AGTTGCAATTCTGAAACACA+AGG + Chr8:28300440-28300459 None:intergenic 35.0%
!! GATGAAATGGTTTGGTTTGT+TGG + Chr8:28299092-28299111 None:intergenic 35.0%
!! GCTTAAACTTCTAGTTCTAG+TGG + Chr8:28299668-28299687 None:intergenic 35.0%
!! TCATCATTACTTGTTGTGGT+TGG + Chr8:28299280-28299299 None:intergenic 35.0%
!!! TGTTAGGGTTTTGATGATGA+GGG + Chr8:28299199-28299218 None:intergenic 35.0%
!!! TTGCAACAAGGTTTTGGTTT+TGG + Chr8:28299005-28299024 None:intergenic 35.0%
!!! TTGTTAGGGTTTTGATGATG+AGG + Chr8:28299200-28299219 None:intergenic 35.0%
AAAGCAATCTCAACAAGCAG+AGG + Chr8:28300537-28300556 None:intergenic 40.0%
AGAATTCAAACCTGTAGCAG+TGG - Chr8:28298818-28298837 MsG0880043643.01.T01:CDS 40.0%
ATGGCCACTTATATTCACAG+TGG + Chr8:28300364-28300383 None:intergenic 40.0%
CTATGAGAACCAAGTCTCTT+AGG + Chr8:28299044-28299063 None:intergenic 40.0%
GATGAAGGTAAGGGAATGTT+TGG + Chr8:28299125-28299144 None:intergenic 40.0%
GATGTAGTGTGAGTGTGATT+TGG + Chr8:28298911-28298930 None:intergenic 40.0%
GTAAAGAGAGGATGAGGTTA+AGG + Chr8:28298955-28298974 None:intergenic 40.0%
GTGAGGAAAATGGTGAGTTT+AGG - Chr8:28299376-28299395 MsG0880043643.01.T01:intron 40.0%
GTTAATAGAAAAATGCCCCG+AGG - Chr8:28300149-28300168 MsG0880043643.01.T01:five_prime_UTR 40.0%
TGCCAATAGCAGATACTCTA+TGG + Chr8:28300314-28300333 None:intergenic 40.0%
TGTTTGTGAATTGCAGATCC+TGG - Chr8:28300237-28300256 MsG0880043643.01.T01:five_prime_UTR 40.0%
TTGGCCACTGTGAATATAAG+TGG - Chr8:28300357-28300376 MsG0880043643.01.T01:five_prime_UTR 40.0%
! ACACTTAGAGCTGATGATGA+AGG + Chr8:28299140-28299159 None:intergenic 40.0%
! AGAGCTGATGATGAAGGTAA+GGG + Chr8:28299134-28299153 None:intergenic 40.0%
! AGAGTATCTGCTATTGGCAA+TGG - Chr8:28300315-28300334 MsG0880043643.01.T01:five_prime_UTR 40.0%
! ATAACTAGCTCTTGTCACCA+AGG - Chr8:28299694-28299713 MsG0880043643.01.T01:CDS 40.0%
! CAACTTGGAAGTAATGGAAG+TGG - Chr8:28300455-28300474 MsG0880043643.01.T01:five_prime_UTR 40.0%
! TAGAGCTGATGATGAAGGTA+AGG + Chr8:28299135-28299154 None:intergenic 40.0%
! TAGGGAGATTTGGAACAGAA+AGG + Chr8:28298846-28298865 None:intergenic 40.0%
! TGCCATAGAGTATCTGCTAT+TGG - Chr8:28300309-28300328 MsG0880043643.01.T01:five_prime_UTR 40.0%
! TGTAATCATAGGTGTCGTGT+CGG - Chr8:28299887-28299906 MsG0880043643.01.T01:five_prime_UTR 40.0%
! TGTTGGTTGTGAAGTGATGA+AGG + Chr8:28299075-28299094 None:intergenic 40.0%
!! AGATGTAGGACTTGCTGTAA+AGG - Chr8:28299445-28299464 MsG0880043643.01.T01:intron 40.0%
!! TCATCATCAGCTCTAAGTGT+TGG - Chr8:28299140-28299159 MsG0880043643.01.T01:intron 40.0%
AATCTCAACAAGCAGAGGCA+GGG + Chr8:28300532-28300551 None:intergenic 45.0%
CACGACACCTATGATTACAC+TGG + Chr8:28299886-28299905 None:intergenic 45.0%
CATGTTAGGCCTAAGAGACT+TGG - Chr8:28299032-28299051 MsG0880043643.01.T01:CDS 45.0%
CTATGGCACTTGAAAACAGC+AGG + Chr8:28300297-28300316 None:intergenic 45.0%
GTCAGAGAGGTAGACACAAT+TGG - Chr8:28300179-28300198 MsG0880043643.01.T01:five_prime_UTR 45.0%
TGTCCGTGTTTCATAGGTCT+CGG + Chr8:28299791-28299810 None:intergenic 45.0%
!! ACTTGATGATGATGGGTGTG+AGG - Chr8:28299359-28299378 MsG0880043643.01.T01:intron 45.0%
!! ATTTAGTCTCAGAGGTGTCC+TGG + Chr8:28299945-28299964 None:intergenic 45.0%
!! GATGATGGGTGTGAGGAAAA+TGG - Chr8:28299366-28299385 MsG0880043643.01.T01:intron 45.0%
!! TCAGCTCTAAGTGTTGGTCT+TGG - Chr8:28299146-28299165 MsG0880043643.01.T01:intron 45.0%
!!! GTTTTGATGATGAGGGTGAG+TGG + Chr8:28299192-28299211 None:intergenic 45.0%
CAATCTCAACAAGCAGAGGC+AGG + Chr8:28300533-28300552 None:intergenic 50.0%
CGAACACGAACATACCGACA+CGG - Chr8:28299834-28299853 MsG0880043643.01.T01:exon 50.0%
CTTCAGCCACCATCATACTC+CGG + Chr8:28299534-28299553 None:intergenic 50.0%
GGCAAAGTCTGCTTGAAACC+AGG + Chr8:28300258-28300277 None:intergenic 50.0%
GGTGAGTGGTGGTTAGTAGT+GGG + Chr8:28299178-28299197 None:intergenic 50.0%
GTCTGGTGTCCGTGTTTCAT+AGG + Chr8:28299797-28299816 None:intergenic 50.0%
GTGCTTCTTCTGGTACCAAG+AGG - Chr8:28299572-28299591 MsG0880043643.01.T01:intron 50.0%
GTTGTGGTTGGCACATCTTG+AGG + Chr8:28299268-28299287 None:intergenic 50.0%
TAGAAAAATGCCCCGAGGCA+CGG - Chr8:28300154-28300173 MsG0880043643.01.T01:five_prime_UTR 50.0%
TGGCCGAGACCTATGAAACA+CGG - Chr8:28299785-28299804 MsG0880043643.01.T01:CDS 50.0%
! ATCGACTTATGTCCGACACC+AGG - Chr8:28299924-28299943 MsG0880043643.01.T01:five_prime_UTR 50.0%
! CATAGGTGTCGTGTCGGTAT+CGG - Chr8:28299893-28299912 MsG0880043643.01.T01:five_prime_UTR 50.0%
!! AGGACTTGCTGTAAAGGTCG+TGG - Chr8:28299451-28299470 MsG0880043643.01.T01:intron 50.0%
!! AGTATGATGGTGGCTGAAGG+TGG - Chr8:28299535-28299554 MsG0880043643.01.T01:intron 50.0%
!! AGTGATGGTGGTGCTTCTTC+TGG - Chr8:28299562-28299581 MsG0880043643.01.T01:intron 50.0%
!! TTGATGATGAGGGTGAGTGG+TGG + Chr8:28299189-28299208 None:intergenic 50.0%
GAAGGTGGTGACAGTGATGG+TGG - Chr8:28299550-28299569 MsG0880043643.01.T01:intron 55.0%
GCTGAAGGTGGTGACAGTGA+TGG - Chr8:28299547-28299566 MsG0880043643.01.T01:intron 55.0%
GGGTGAGTGGTGGTTAGTAG+TGG + Chr8:28299179-28299198 None:intergenic 55.0%
TGTCGTGTTCGTGTCGTGTC+TGG + Chr8:28299814-28299833 None:intergenic 55.0%
! CGTGAAGAGCACGTGGATAC+CGG - Chr8:28299492-28299511 MsG0880043643.01.T01:intron 55.0%
! GTACTCACGTGAAGAGCACG+TGG - Chr8:28299485-28299504 MsG0880043643.01.T01:intron 55.0%
!! CAGAAAGGCACCACTGCTAC+AGG + Chr8:28298831-28298850 None:intergenic 55.0%
!! CGGAGTATGATGGTGGCTGA+AGG - Chr8:28299532-28299551 MsG0880043643.01.T01:intron 55.0%
!! GATACCGGCTTCGATGAGAC+GGG - Chr8:28299507-28299526 MsG0880043643.01.T01:intron 55.0%
!! GGATACCGGCTTCGATGAGA+CGG - Chr8:28299506-28299525 MsG0880043643.01.T01:intron 55.0%
!! TCTCAGAGGTGTCCTGGTGT+CGG + Chr8:28299939-28299958 None:intergenic 55.0%
!! TGTCGTGTCGGTATCGGTGT+TGG - Chr8:28299899-28299918 MsG0880043643.01.T01:five_prime_UTR 55.0%
ACGGGACCGGAGTATGATGG+TGG - Chr8:28299525-28299544 MsG0880043643.01.T01:intron 60.0%
GAGACGGGACCGGAGTATGA+TGG - Chr8:28299522-28299541 MsG0880043643.01.T01:intron 60.0%
CCTCTCTGACGACCGTGCCT+CGG + Chr8:28300169-28300188 None:intergenic 65.0%
CGGCTTCGATGAGACGGGAC+CGG - Chr8:28299512-28299531 MsG0880043643.01.T01:intron 65.0%
CGGTCCCGTCTCATCGAAGC+CGG + Chr8:28299514-28299533 None:intergenic 65.0%
CTCTCTGACGACCGTGCCTC+GGG + Chr8:28300168-28300187 None:intergenic 65.0%
TCTCTGACGACCGTGCCTCG+GGG + Chr8:28300167-28300186 None:intergenic 65.0%
CCGAGGCACGGTCGTCAGAG+AGG - Chr8:28300166-28300185 MsG0880043643.01.T01:five_prime_UTR 70.0%
GCAGGCGCGCGAACATAGCG+AGG + Chr8:28300279-28300298 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr8 gene 28298796 28300563 28298796 ID=MsG0880043643.01;Name=MsG0880043643.01
Chr8 mRNA 28298796 28300563 28298796 ID=MsG0880043643.01.T01;Parent=MsG0880043643.01;Name=MsG0880043643.01.T01;_AED=0.49;_eAED=0.50;_QI=724|1|0.5|1|1|1|2|0|168
Chr8 exon 28298796 28299106 28298796 ID=MsG0880043643.01.T01:exon:14882;Parent=MsG0880043643.01.T01
Chr8 exon 28299644 28300563 28299644 ID=MsG0880043643.01.T01:exon:14881;Parent=MsG0880043643.01.T01
Chr8 five_prime_UTR 28299840 28300563 28299840 ID=MsG0880043643.01.T01:five_prime_utr;Parent=MsG0880043643.01.T01
Chr8 CDS 28299644 28299839 28299644 ID=MsG0880043643.01.T01:cds;Parent=MsG0880043643.01.T01
Chr8 CDS 28298796 28299106 28298796 ID=MsG0880043643.01.T01:cds;Parent=MsG0880043643.01.T01
Gene Sequence

>MsG0880043643.01.T01

ATGAGACGGGACCGGAGTATGATGGTGGCTGAAGGTGGTGACAGTGATGGTGGTGCTTCTTCTGGTACCAAGAGGCAGAGAATTTTGATTTCATCATCACAGAATAATGCTGCTGCTAACTCTCATAGTTCTAGTTCTAATGCTGCCACTAGAACTAGAAGTTTAAGCTTAGATATAACTAGCTCTTGTCACCAAGATCCTGGTTTCAAGCAGACTTTGCCTCGCTATGTTCGCGCGCCTGCTGTTTTCAAGTGCCATAGAGTATCTGCTATTGGCAATGGTGAAGATGAACTTGCTTACTTGGCCACTGTGAATATAAGTGGCCATGTATTCAAAGGTTTCTCTATGATCAAGGTGTTGATGACAAAAATGAAACAATGCCTTGTGTTTCAGAATTGCAACTTGGAAGTAATGGAAGTGGAAAGAGTAATAGATGTTCTTCTTCTGCAATTGAGGTTCAAACTAATGCTTACCCTGCCTCTGCTTGTTGAGATTGCTTTAAGGTAA
Protein sequence

>MsG0880043643.01.T01

MRRDRSMMVAEGGDSDGGASSGTKRQRILISSSQNNAAANSHSSSSNAATRTRSLSLDITSSCHQDPGFKQTLPRYVRAPAVFKCHRVSAIGNGEDELAYLATVNISGHVFKGFSMIKVLMTKMKQCLVFQNCNLEVMEVERVIDVLLLQLRFKLMLTLPLLVEIALR*