AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043777.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043777.01.T01 MTR_8g040900 93.913 230 2 2 1 220 1 228 4.48e-159 439
MsG0880043777.01.T01 MTR_3g077240 75.221 113 25 1 1 110 1 113 4.47e-58 182
MsG0880043777.01.T01 MTR_4g105170 66.929 127 32 1 5 121 9 135 1.42e-57 181
MsG0880043777.01.T01 MTR_7g033800 69.912 113 34 0 2 114 6 118 3.95e-57 179
MsG0880043777.01.T01 MTR_5g017950 73.394 109 29 0 2 110 3 111 9.25e-54 175
MsG0880043777.01.T01 MTR_6g005070 69.643 112 32 1 1 110 1 112 1.57e-53 169
MsG0880043777.01.T01 MTR_6g005080 68.750 112 33 1 1 110 2 113 8.81e-53 167
MsG0880043777.01.T01 MTR_3g452660 69.091 110 34 0 1 110 1 110 1.01e-50 166
MsG0880043777.01.T01 MTR_3g073690 62.903 124 43 2 1 121 2 125 4.80e-49 159
MsG0880043777.01.T01 MTR_5g083230 63.551 107 39 0 5 111 4 110 1.11e-46 152
MsG0880043777.01.T01 MTR_3g031660 58.333 120 50 0 1 120 1 120 2.54e-46 151
MsG0880043777.01.T01 MTR_4g083680 65.347 101 35 0 6 106 14 114 4.58e-45 148
MsG0880043777.01.T01 MTR_5g083010 61.321 106 41 0 4 109 8 113 3.43e-43 144
MsG0880043777.01.T01 MTR_2g068760 60.784 102 40 0 6 107 12 113 1.79e-42 141
MsG0880043777.01.T01 MTR_5g083960 51.200 125 61 0 4 128 36 160 5.27e-42 141
MsG0880043777.01.T01 MTR_3g071590 59.615 104 42 0 4 107 5 108 5.72e-42 140
MsG0880043777.01.T01 MTR_3g071420 61.386 101 39 0 4 104 11 111 4.92e-41 138
MsG0880043777.01.T01 MTR_3g094690 56.604 106 46 0 6 111 46 151 8.95e-41 139
MsG0880043777.01.T01 MTR_2g093310 54.717 106 48 0 6 111 32 137 9.82e-39 133
MsG0880043777.01.T01 MTR_3g094690 56.604 106 45 1 6 111 46 150 1.08e-38 134
MsG0880043777.01.T01 MTR_5g075020 51.200 125 61 0 6 130 42 166 1.21e-37 131
MsG0880043777.01.T01 MTR_5g075020 51.200 125 61 0 6 130 46 170 2.57e-37 130
MsG0880043777.01.T01 MTR_7g096530 56.897 116 42 2 1 108 1 116 3.77e-37 129
MsG0880043777.01.T01 MTR_4g060950 55.340 103 45 1 6 107 23 125 2.23e-36 126
MsG0880043777.01.T01 MTR_1g095850 57.000 100 43 0 8 107 73 172 2.04e-35 125
MsG0880043777.01.T01 MTR_8g079660 47.826 115 60 0 7 121 8 122 6.44e-35 125
MsG0880043777.01.T01 MTR_7g028905 50.485 103 51 0 6 108 4 106 6.13e-34 118
MsG0880043777.01.T01 MTR_1g070220 51.724 116 55 1 3 117 11 126 1.78e-33 119
MsG0880043777.01.T01 MTR_8g036085 52.830 106 49 1 7 111 23 128 9.19e-33 118
MsG0880043777.01.T01 MTR_8g036105 54.054 111 50 1 7 116 18 128 1.85e-32 117
MsG0880043777.01.T01 MTR_8g079620 43.262 141 69 2 7 141 8 143 1.85e-30 112
MsG0880043777.01.T01 MTR_7g074990 57.895 95 39 1 7 100 20 114 4.31e-30 112
MsG0880043777.01.T01 MTR_4g107450 48.000 100 52 0 8 107 11 110 6.66e-30 112
MsG0880043777.01.T01 MTR_6g027710 49.515 103 51 1 6 107 39 141 9.26e-30 110
MsG0880043777.01.T01 MTR_5g093010 44.248 113 61 1 8 118 6 118 2.23e-29 107
MsG0880043777.01.T01 MTR_5g080470 49.505 101 50 1 1 100 1 101 2.81e-29 108
MsG0880043777.01.T01 MTR_7g096610 53.465 101 46 1 1 100 2 102 3.34e-28 106
MsG0880043777.01.T01 MTR_6g011250 41.935 124 64 2 1 123 3 119 5.12e-28 105
MsG0880043777.01.T01 MTR_6g011200 38.621 145 77 4 1 133 3 147 2.17e-27 105
MsG0880043777.01.T01 MTR_6g011230 42.574 101 58 0 4 104 8 108 2.51e-27 103
MsG0880043777.01.T01 MTR_1g070205 52.632 95 44 1 7 100 12 106 2.66e-27 104
MsG0880043777.01.T01 MTR_4g099230 37.762 143 78 4 8 148 3 136 1.43e-26 100
MsG0880043777.01.T01 MTR_7g075230 49.550 111 53 2 7 114 12 122 2.33e-26 100
MsG0880043777.01.T01 MTR_8g079580 45.455 110 59 1 7 115 8 117 9.72e-26 100
MsG0880043777.01.T01 MTR_8g017090 40.385 104 62 0 8 111 13 116 2.73e-25 100
MsG0880043777.01.T01 MTR_6g027700 42.056 107 61 1 1 106 1 107 1.11e-24 97.1
MsG0880043777.01.T01 MTR_2g100020 36.508 126 76 1 8 133 20 141 4.40e-24 97.1
MsG0880043777.01.T01 MTR_5g085390 41.228 114 66 1 1 113 3 116 1.59e-22 92.0
MsG0880043777.01.T01 MTR_4g088035 33.721 86 57 0 8 93 7 92 1.73e-15 73.6
MsG0880043777.01.T02 MTR_8g040900 98.261 230 2 1 1 230 1 228 1.22e-169 466
MsG0880043777.01.T02 MTR_4g105170 66.929 127 42 0 5 131 9 135 4.18e-59 185
MsG0880043777.01.T02 MTR_3g077240 75.221 113 25 1 1 110 1 113 4.47e-58 182
MsG0880043777.01.T02 MTR_7g033800 69.912 113 34 0 2 114 6 118 3.35e-57 179
MsG0880043777.01.T02 MTR_5g017950 73.394 109 29 0 2 110 3 111 9.76e-54 175
MsG0880043777.01.T02 MTR_6g005070 69.643 112 32 1 1 110 1 112 1.46e-53 169
MsG0880043777.01.T02 MTR_6g005080 68.750 112 33 1 1 110 2 113 9.62e-53 167
MsG0880043777.01.T02 MTR_3g452660 69.091 110 34 0 1 110 1 110 9.65e-51 167
MsG0880043777.01.T02 MTR_3g073690 67.568 111 35 1 1 110 2 112 1.74e-48 158
MsG0880043777.01.T02 MTR_5g083230 63.551 107 39 0 5 111 4 110 1.56e-46 152
MsG0880043777.01.T02 MTR_3g031660 62.617 107 40 0 1 107 1 107 6.89e-46 150
MsG0880043777.01.T02 MTR_4g083680 65.347 101 35 0 6 106 14 114 5.81e-45 148
MsG0880043777.01.T02 MTR_5g083010 61.321 106 41 0 4 109 8 113 4.29e-43 144
MsG0880043777.01.T02 MTR_2g068760 60.784 102 40 0 6 107 12 113 2.93e-42 141
MsG0880043777.01.T02 MTR_5g083960 42.949 156 89 0 4 159 36 191 7.09e-42 141
MsG0880043777.01.T02 MTR_3g071590 59.615 104 42 0 4 107 5 108 8.11e-42 140
MsG0880043777.01.T02 MTR_3g071420 61.386 101 39 0 4 104 11 111 6.47e-41 138
MsG0880043777.01.T02 MTR_3g094690 56.604 106 46 0 6 111 46 151 1.15e-40 139
MsG0880043777.01.T02 MTR_3g094690 56.604 106 45 1 6 111 46 150 1.44e-38 134
MsG0880043777.01.T02 MTR_2g093310 54.717 106 48 0 6 111 32 137 1.72e-38 132
MsG0880043777.01.T02 MTR_5g075020 58.491 106 44 0 6 111 42 147 2.43e-37 130
MsG0880043777.01.T02 MTR_5g075020 58.491 106 44 0 6 111 46 151 4.97e-37 130
MsG0880043777.01.T02 MTR_7g096530 56.897 116 42 2 1 108 1 116 5.58e-37 129
MsG0880043777.01.T02 MTR_4g060950 55.340 103 45 1 6 107 23 125 3.19e-36 126
MsG0880043777.01.T02 MTR_1g095850 57.000 100 43 0 8 107 73 172 2.64e-35 125
MsG0880043777.01.T02 MTR_8g079660 49.091 110 56 0 7 116 8 117 1.58e-34 124
MsG0880043777.01.T02 MTR_7g028905 50.485 103 51 0 6 108 4 106 8.39e-34 118
MsG0880043777.01.T02 MTR_1g070220 52.679 112 52 1 3 113 11 122 4.17e-33 119
MsG0880043777.01.T02 MTR_8g036105 53.043 115 53 1 7 120 18 132 1.09e-32 118
MsG0880043777.01.T02 MTR_8g036085 52.830 106 49 1 7 111 23 128 1.33e-32 118
MsG0880043777.01.T02 MTR_4g107450 39.744 156 79 3 8 154 11 160 2.15e-30 114
MsG0880043777.01.T02 MTR_8g079620 46.087 115 62 0 7 121 8 122 3.60e-30 112
MsG0880043777.01.T02 MTR_7g074990 57.895 95 39 1 7 100 20 114 6.65e-30 111
MsG0880043777.01.T02 MTR_6g027710 49.515 103 51 1 6 107 39 141 1.84e-29 110
MsG0880043777.01.T02 MTR_5g093010 50.538 93 46 0 8 100 6 98 4.18e-29 107
MsG0880043777.01.T02 MTR_5g080470 49.505 101 50 1 1 100 1 101 4.54e-29 108
MsG0880043777.01.T02 MTR_7g096610 53.465 101 46 1 1 100 2 102 7.57e-28 105
MsG0880043777.01.T02 MTR_6g011250 43.860 114 56 2 1 113 3 109 1.05e-27 104
MsG0880043777.01.T02 MTR_6g011200 40.000 130 69 3 1 121 3 132 1.70e-27 105
MsG0880043777.01.T02 MTR_6g011230 42.574 101 58 0 4 104 8 108 3.63e-27 103
MsG0880043777.01.T02 MTR_1g070205 48.246 114 56 2 7 119 12 123 4.36e-27 104
MsG0880043777.01.T02 MTR_4g099230 40.476 126 59 3 8 117 3 128 4.82e-26 99.4
MsG0880043777.01.T02 MTR_7g075230 49.550 111 53 2 7 114 12 122 5.18e-26 100
MsG0880043777.01.T02 MTR_8g079580 45.455 110 59 1 7 115 8 117 1.05e-25 100
MsG0880043777.01.T02 MTR_8g017090 40.385 104 62 0 8 111 13 116 2.73e-25 100
MsG0880043777.01.T02 MTR_6g027700 42.056 107 61 1 1 106 1 107 2.09e-24 96.7
MsG0880043777.01.T02 MTR_2g100020 39.604 101 61 0 8 108 20 120 9.68e-24 96.3
MsG0880043777.01.T02 MTR_5g085390 41.228 114 66 1 1 113 3 116 2.65e-22 91.7
MsG0880043777.01.T02 MTR_4g088035 33.721 86 57 0 8 93 7 92 1.72e-15 73.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043777.01.T01 AT1G65620 62.557 219 59 5 2 220 4 199 8.27e-77 230
MsG0880043777.01.T01 AT1G65620 62.557 219 59 5 2 220 4 199 8.27e-77 230
MsG0880043777.01.T01 AT1G65620 62.557 219 59 5 2 220 4 199 8.27e-77 230
MsG0880043777.01.T01 AT1G65620 62.557 219 59 5 2 220 4 199 8.27e-77 230
MsG0880043777.01.T01 AT1G65620 62.557 219 59 5 2 220 4 199 8.27e-77 230
MsG0880043777.01.T01 AT5G63090 67.969 128 37 1 1 124 1 128 1.41e-56 178
MsG0880043777.01.T01 AT5G63090 67.969 128 37 1 1 124 1 128 1.41e-56 178
MsG0880043777.01.T01 AT5G63090 67.969 128 37 1 1 124 1 128 1.41e-56 178
MsG0880043777.01.T01 AT5G63090 67.969 128 37 1 1 124 1 128 1.41e-56 178
MsG0880043777.01.T01 AT5G66870 75.455 110 27 0 1 110 1 110 1.21e-55 180
MsG0880043777.01.T01 AT3G27650 63.492 126 41 1 4 129 36 156 1.59e-54 172
MsG0880043777.01.T01 AT2G23660 70.192 104 31 0 4 107 2 105 1.62e-50 166
MsG0880043777.01.T01 AT2G23660 70.192 104 31 0 4 107 2 105 1.62e-50 166
MsG0880043777.01.T01 AT2G23660 70.192 104 31 0 4 107 2 105 1.62e-50 166
MsG0880043777.01.T01 AT2G30130 63.551 107 39 0 5 111 6 112 4.62e-46 151
MsG0880043777.01.T01 AT1G31320 69.318 88 27 0 6 93 12 99 7.66e-42 140
MsG0880043777.01.T01 AT2G30340 53.448 116 52 1 6 119 51 166 3.35e-41 141
MsG0880043777.01.T01 AT2G30340 53.448 116 52 1 6 119 52 167 3.51e-41 141
MsG0880043777.01.T01 AT1G07900 57.547 106 45 0 6 111 32 137 1.03e-39 135
MsG0880043777.01.T01 AT3G11090 56.731 104 45 0 4 107 8 111 9.69e-39 132
MsG0880043777.01.T01 AT1G16530 57.798 109 45 1 6 113 13 121 1.58e-38 131
MsG0880043777.01.T01 AT2G28500 56.604 106 46 0 6 111 54 159 5.26e-38 132
MsG0880043777.01.T01 AT2G40470 51.613 124 60 0 6 129 19 142 1.35e-37 130
MsG0880043777.01.T01 AT2G40470 51.613 124 60 0 6 129 44 167 1.53e-37 130
MsG0880043777.01.T01 AT3G26660 52.679 112 46 1 8 112 6 117 3.91e-37 126
MsG0880043777.01.T01 AT3G26620 53.333 105 49 0 8 112 6 110 5.01e-37 126
MsG0880043777.01.T01 AT3G03760 55.340 103 45 1 6 107 50 152 2.61e-33 121
MsG0880043777.01.T01 AT2G42430 45.255 137 61 2 2 137 10 133 6.12e-33 119
MsG0880043777.01.T01 AT4G00210 54.630 108 48 1 7 113 11 118 6.91e-33 118
MsG0880043777.01.T01 AT3G50510 48.182 110 57 0 1 110 6 115 8.46e-33 117
MsG0880043777.01.T01 AT3G50510 48.182 110 57 0 1 110 6 115 8.46e-33 117
MsG0880043777.01.T01 AT5G06080 50.450 111 54 1 1 110 1 111 3.74e-30 110
MsG0880043777.01.T01 AT2G31310 49.091 110 55 1 1 109 1 110 1.19e-29 109
MsG0880043777.01.T01 AT2G45420 51.456 103 49 1 10 111 40 142 1.33e-29 111
MsG0880043777.01.T01 AT3G58190 41.667 156 67 5 7 154 11 150 2.34e-29 109
MsG0880043777.01.T01 AT4G00220 52.427 103 48 1 10 111 20 122 2.35e-29 109
MsG0880043777.01.T01 AT2G45410 41.060 151 71 2 10 142 19 169 2.52e-29 108
MsG0880043777.01.T01 AT5G35900 48.113 106 55 0 8 113 6 111 2.97e-29 108
MsG0880043777.01.T01 AT2G42440 47.273 110 57 1 1 109 1 110 5.45e-28 106
MsG0880043777.01.T01 AT3G13850 40.952 105 62 0 8 112 37 141 5.08e-27 104
MsG0880043777.01.T01 AT3G47870 40.000 105 63 0 8 112 37 141 1.16e-25 102
MsG0880043777.01.T01 AT1G72980 37.838 111 69 0 4 114 10 120 8.67e-21 87.0
MsG0880043777.01.T01 AT4G22700 40.385 104 60 2 8 110 6 108 1.68e-19 83.2
MsG0880043777.01.T01 AT1G06280 39.080 87 53 0 8 94 25 111 1.86e-18 80.5
MsG0880043777.01.T01 AT2G19820 33.708 89 58 1 6 93 11 99 2.87e-14 67.4
MsG0880043777.01.T01 AT5G15060 39.726 73 42 1 8 78 18 90 1.41e-13 65.9
MsG0880043777.01.T01 AT3G27940 42.424 66 38 0 7 72 5 70 4.80e-11 59.3
MsG0880043777.01.T01 AT3G27940 42.424 66 38 0 7 72 5 70 4.80e-11 59.3
MsG0880043777.01.T01 AT3G27940 42.424 66 38 0 7 72 5 70 4.80e-11 59.3
MsG0880043777.01.T02 AT1G65620 60.262 229 58 5 2 230 4 199 3.84e-77 231
MsG0880043777.01.T02 AT1G65620 60.262 229 58 5 2 230 4 199 3.84e-77 231
MsG0880043777.01.T02 AT1G65620 60.262 229 58 5 2 230 4 199 3.84e-77 231
MsG0880043777.01.T02 AT1G65620 60.262 229 58 5 2 230 4 199 3.84e-77 231
MsG0880043777.01.T02 AT1G65620 60.262 229 58 5 2 230 4 199 3.84e-77 231
MsG0880043777.01.T02 AT5G63090 69.291 127 34 2 1 123 1 126 1.26e-56 178
MsG0880043777.01.T02 AT5G63090 69.291 127 34 2 1 123 1 126 1.26e-56 178
MsG0880043777.01.T02 AT5G63090 69.291 127 34 2 1 123 1 126 1.26e-56 178
MsG0880043777.01.T02 AT5G63090 69.291 127 34 2 1 123 1 126 1.26e-56 178
MsG0880043777.01.T02 AT5G66870 75.455 110 27 0 1 110 1 110 1.29e-55 180
MsG0880043777.01.T02 AT3G27650 70.093 107 32 0 4 110 36 142 1.05e-53 170
MsG0880043777.01.T02 AT2G23660 70.192 104 31 0 4 107 2 105 1.44e-50 167
MsG0880043777.01.T02 AT2G23660 70.192 104 31 0 4 107 2 105 1.44e-50 167
MsG0880043777.01.T02 AT2G23660 70.192 104 31 0 4 107 2 105 1.44e-50 167
MsG0880043777.01.T02 AT2G30130 63.551 107 39 0 5 111 6 112 6.63e-46 151
MsG0880043777.01.T02 AT1G31320 69.318 88 27 0 6 93 12 99 9.04e-42 140
MsG0880043777.01.T02 AT2G30340 57.692 104 44 0 6 109 52 155 8.61e-41 140
MsG0880043777.01.T02 AT2G30340 57.692 104 44 0 6 109 51 154 9.45e-41 140
MsG0880043777.01.T02 AT1G07900 57.547 106 45 0 6 111 32 137 1.66e-39 135
MsG0880043777.01.T02 AT3G11090 56.731 104 45 0 4 107 8 111 1.57e-38 132
MsG0880043777.01.T02 AT1G16530 57.798 109 45 1 6 113 13 121 2.19e-38 131
MsG0880043777.01.T02 AT2G28500 56.604 106 46 0 6 111 54 159 7.10e-38 132
MsG0880043777.01.T02 AT2G40470 57.547 106 45 0 6 111 19 124 2.76e-37 129
MsG0880043777.01.T02 AT2G40470 51.200 125 60 1 6 129 44 168 4.01e-37 130
MsG0880043777.01.T02 AT3G26660 52.679 112 46 1 8 112 6 117 5.01e-37 126
MsG0880043777.01.T02 AT3G26620 53.333 105 49 0 8 112 6 110 5.96e-37 126
MsG0880043777.01.T02 AT3G03760 55.340 103 45 1 6 107 50 152 3.95e-33 121
MsG0880043777.01.T02 AT4G00210 54.630 108 48 1 7 113 11 118 1.16e-32 118
MsG0880043777.01.T02 AT3G50510 48.182 110 57 0 1 110 6 115 1.39e-32 117
MsG0880043777.01.T02 AT3G50510 48.182 110 57 0 1 110 6 115 1.39e-32 117
MsG0880043777.01.T02 AT2G42430 50.893 112 54 1 2 112 10 121 1.88e-32 118
MsG0880043777.01.T02 AT5G06080 50.450 111 54 1 1 110 1 111 5.23e-30 110
MsG0880043777.01.T02 AT2G45410 41.722 151 80 2 10 152 19 169 1.23e-29 109
MsG0880043777.01.T02 AT2G45420 51.456 103 49 1 10 111 40 142 1.94e-29 111
MsG0880043777.01.T02 AT2G31310 49.091 110 55 1 1 109 1 110 2.15e-29 108
MsG0880043777.01.T02 AT4G00220 52.427 103 48 1 10 111 20 122 2.95e-29 109
MsG0880043777.01.T02 AT5G35900 48.113 106 55 0 8 113 6 111 4.66e-29 108
MsG0880043777.01.T02 AT3G58190 49.038 104 52 1 7 109 11 114 4.14e-28 106
MsG0880043777.01.T02 AT2G42440 47.273 110 57 1 1 109 1 110 8.24e-28 106
MsG0880043777.01.T02 AT3G13850 40.952 105 62 0 8 112 37 141 7.33e-27 104
MsG0880043777.01.T02 AT3G47870 40.000 105 63 0 8 112 37 141 1.35e-25 102
MsG0880043777.01.T02 AT1G72980 37.838 111 69 0 4 114 10 120 1.76e-20 86.3
MsG0880043777.01.T02 AT4G22700 40.385 104 60 2 8 110 6 108 2.15e-19 82.8
MsG0880043777.01.T02 AT1G06280 39.080 87 53 0 8 94 25 111 1.77e-18 80.9
MsG0880043777.01.T02 AT2G19820 32.584 89 59 1 6 93 11 99 3.88e-14 67.4
MsG0880043777.01.T02 AT5G15060 39.726 73 42 1 8 78 18 90 1.20e-13 66.2
MsG0880043777.01.T02 AT3G27940 42.424 66 38 0 7 72 5 70 4.88e-11 59.7
MsG0880043777.01.T02 AT3G27940 42.424 66 38 0 7 72 5 70 4.88e-11 59.7
MsG0880043777.01.T02 AT3G27940 42.424 66 38 0 7 72 5 70 4.88e-11 59.7

Find 45 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGACTTCGTGATCCTGTTTA+TGG 0.153983 8:+30446286 MsG0880043777.01.T02:CDS
TCAACATCAACAACATAATT+TGG 0.253618 8:+30446585 MsG0880043777.01.T02:CDS
CACATTCTGGTTGGCATTTG+CGG 0.315598 8:-30446111 None:intergenic
GTGCTAAATCCGAACTTTCT+CGG 0.374243 8:+30446371 MsG0880043777.01.T02:CDS
TAAGGTGCAAATGCACATTC+TGG 0.376608 8:-30446124 None:intergenic
GGCTGATCAGGTGGAAAATA+AGG 0.377629 8:-30446142 None:intergenic
ATGAATATTTCTGCTAGCTT+AGG 0.398380 8:+30446661 MsG0880043777.01.T02:CDS
ATCGCAGCAGCAGCTACTGC+CGG 0.415834 8:+30446409 MsG0880043777.01.T02:CDS
GCCGGTCATGGACTCATAAC+CGG 0.444703 8:+30446427 MsG0880043777.01.T02:CDS
CATAGTGCTGCTGGTGGTGG+TGG 0.453028 8:+30446706 MsG0880043777.01.T02:CDS
TGTTGTTGATGTTGATCCCT+TGG 0.455156 8:-30446577 None:intergenic
ACACCAACACAACCATAAAC+AGG 0.455574 8:-30446298 None:intergenic
CCAACTTCGTCAGCTTCAAA+TGG 0.466283 8:+30446339 MsG0880043777.01.T02:CDS
CTTCAACATCATAGTGCTGC+TGG 0.473475 8:+30446697 MsG0880043777.01.T02:CDS
GAATCTGTCACGGCCGCTGC+AGG 0.483621 8:+30446460 MsG0880043777.01.T02:CDS
CAACTATGATGCTAGTCTCT+TGG 0.489577 8:+30446636 MsG0880043777.01.T02:CDS
GGACTCATAACCGGCATGAC+AGG 0.499561 8:+30446436 MsG0880043777.01.T02:CDS
GCAGCAGCTACTGCCGGTCA+TGG 0.500625 8:+30446415 MsG0880043777.01.T02:CDS
CATCATAGTGCTGCTGGTGG+TGG 0.501541 8:+30446703 MsG0880043777.01.T02:CDS
GCTGACGAAGTTGGTGTTGA+AGG 0.501764 8:-30446330 None:intergenic
GCCGGTTATGAGTCCATGAC+CGG 0.516685 8:-30446428 None:intergenic
CCATTTGAAGCTGACGAAGT+TGG 0.517758 8:-30446339 None:intergenic
CACCACACGCAGAACACTGC+TGG 0.529976 8:+30446490 MsG0880043777.01.T02:CDS
TGCTGCACATGGTGAATTTG+AGG 0.533620 8:-30446074 None:intergenic
GACAGATTCACCTGTCATGC+CGG 0.536335 8:-30446446 None:intergenic
GATCCTGTTTATGGTTGTGT+TGG 0.542374 8:+30446295 MsG0880043777.01.T02:CDS
CAGCATCTTCACGTTGGTGA+GGG 0.561735 8:-30446234 None:intergenic
GCTAGGAGCGGTTAACAGTA+CGG 0.578504 8:-30446738 None:intergenic
ACAGCATCTTCACGTTGGTG+AGG 0.580209 8:-30446235 None:intergenic
CACCAGCAGTGTTCTGCGTG+TGG 0.585016 8:-30446492 None:intergenic
GTGCAAATGCACATTCTGGT+TGG 0.592305 8:-30446120 None:intergenic
ACGCAGAACACTGCTGGTGG+TGG 0.597835 8:+30446496 MsG0880043777.01.T02:CDS
GGTGGTGGTAATAACACTAA+CGG 0.619323 8:+30446511 MsG0880043777.01.T02:CDS
CACACGCAGAACACTGCTGG+TGG 0.628083 8:+30446493 MsG0880043777.01.T02:CDS
TGGTGAATTTGAGGATGCCA+TGG 0.633685 8:-30446065 None:intergenic
AGAAACTTGCATGCTGCACA+TGG 0.636607 8:-30446085 None:intergenic
CATGACAGGTGAATCTGTCA+CGG 0.646689 8:+30446450 MsG0880043777.01.T02:CDS
CAGCAGTGTTCTGCGTGTGG+TGG 0.653991 8:-30446489 None:intergenic
AATTAACAGCATCTTCACGT+TGG 0.680722 8:-30446240 None:intergenic
GATGATGATAACGATGGTCA+CGG 0.691534 8:-30446534 None:intergenic
GTGGTGGTAATTACCTGCAG+CGG 0.695355 8:-30446473 None:intergenic
TTAGAAACCCTAGCTAGGAG+CGG 0.696119 8:-30446750 None:intergenic
CAACATCATAGTGCTGCTGG+TGG 0.709794 8:+30446700 MsG0880043777.01.T02:CDS
GTTGATGATGATGATAACGA+TGG 0.728022 8:-30446540 None:intergenic
AGGAGCGGTTAACAGTACGG+CGG 0.830864 8:-30446735 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTGGTAAGAAATTTTGA+TGG + Chr8:30446601-30446620 MsG0880043777.01.T02:CDS 20.0%
! TCAACATCAACAACATAATT+TGG + Chr8:30446585-30446604 MsG0880043777.01.T02:CDS 25.0%
!! GCAAATGTTCATAGAATTTT+TGG + Chr8:30446175-30446194 MsG0880043777.01.T02:CDS 25.0%
!! TGAACATTTGCAAATTTTTG+TGG - Chr8:30446166-30446185 None:intergenic 25.0%
! ATGAATATTTCTGCTAGCTT+AGG + Chr8:30446661-30446680 MsG0880043777.01.T02:CDS 30.0%
!! TTGGTAAGAAATTTTGATGG+TGG + Chr8:30446604-30446623 MsG0880043777.01.T02:CDS 30.0%
!!! AACATCATCAGTTTTTTCCA+AGG + Chr8:30446560-30446579 MsG0880043777.01.T02:CDS 30.0%
!!! ACATCATCAGTTTTTTCCAA+GGG + Chr8:30446561-30446580 MsG0880043777.01.T02:CDS 30.0%
!!! AGAAATTTTGATGGTGGAAT+TGG + Chr8:30446610-30446629 MsG0880043777.01.T02:CDS 30.0%
!!! GAAATTTTGATGGTGGAATT+GGG + Chr8:30446611-30446630 MsG0880043777.01.T02:CDS 30.0%
AATTAACAGCATCTTCACGT+TGG - Chr8:30446243-30446262 None:intergenic 35.0%
!! GTTGATGATGATGATAACGA+TGG - Chr8:30446543-30446562 None:intergenic 35.0%
!!! GTTTTGAAACCGAGAAAGTT+CGG - Chr8:30446383-30446402 None:intergenic 35.0%
ACACCAACACAACCATAAAC+AGG - Chr8:30446301-30446320 None:intergenic 40.0%
GATCCTGTTTATGGTTGTGT+TGG + Chr8:30446295-30446314 MsG0880043777.01.T02:CDS 40.0%
GTGCTAAATCCGAACTTTCT+CGG + Chr8:30446371-30446390 MsG0880043777.01.T02:CDS 40.0%
TAAGGTGCAAATGCACATTC+TGG - Chr8:30446127-30446146 None:intergenic 40.0%
TGTTGTTGATGTTGATCCCT+TGG - Chr8:30446580-30446599 None:intergenic 40.0%
! AATTTTTGTGGCTGATCAGG+TGG - Chr8:30446154-30446173 None:intergenic 40.0%
! CAACTATGATGCTAGTCTCT+TGG + Chr8:30446636-30446655 MsG0880043777.01.T02:CDS 40.0%
! GATGATGATAACGATGGTCA+CGG - Chr8:30446537-30446556 None:intergenic 40.0%
! GCAAATTTTTGTGGCTGATC+AGG - Chr8:30446157-30446176 None:intergenic 40.0%
! GGTGGTGGTAATAACACTAA+CGG + Chr8:30446511-30446530 MsG0880043777.01.T02:CDS 40.0%
AGAAACTTGCATGCTGCACA+TGG - Chr8:30446088-30446107 None:intergenic 45.0%
CATGACAGGTGAATCTGTCA+CGG + Chr8:30446450-30446469 MsG0880043777.01.T02:CDS 45.0%
CCAACTTCGTCAGCTTCAAA+TGG + Chr8:30446339-30446358 MsG0880043777.01.T02:CDS 45.0%
CGACTTCGTGATCCTGTTTA+TGG + Chr8:30446286-30446305 MsG0880043777.01.T02:CDS 45.0%
CTTCAACATCATAGTGCTGC+TGG + Chr8:30446697-30446716 MsG0880043777.01.T02:CDS 45.0%
GGCTGATCAGGTGGAAAATA+AGG - Chr8:30446145-30446164 None:intergenic 45.0%
GTGCAAATGCACATTCTGGT+TGG - Chr8:30446123-30446142 None:intergenic 45.0%
TGCTGCACATGGTGAATTTG+AGG - Chr8:30446077-30446096 None:intergenic 45.0%
! CACATTCTGGTTGGCATTTG+CGG - Chr8:30446114-30446133 None:intergenic 45.0%
! CCATTTGAAGCTGACGAAGT+TGG - Chr8:30446342-30446361 None:intergenic 45.0%
ACAGCATCTTCACGTTGGTG+AGG - Chr8:30446238-30446257 None:intergenic 50.0%
GACAGATTCACCTGTCATGC+CGG - Chr8:30446449-30446468 None:intergenic 50.0%
GTGGTGGTAATTACCTGCAG+CGG - Chr8:30446476-30446495 None:intergenic 50.0%
! CAACATCATAGTGCTGCTGG+TGG + Chr8:30446700-30446719 MsG0880043777.01.T02:CDS 50.0%
! CAGCATCTTCACGTTGGTGA+GGG - Chr8:30446237-30446256 None:intergenic 50.0%
!! GCTGACGAAGTTGGTGTTGA+AGG - Chr8:30446333-30446352 None:intergenic 50.0%
AGGAGCGGTTAACAGTACGG+CGG - Chr8:30446738-30446757 None:intergenic 55.0%
GCCGGTCATGGACTCATAAC+CGG + Chr8:30446427-30446446 MsG0880043777.01.T02:CDS 55.0%
GCCGGTTATGAGTCCATGAC+CGG - Chr8:30446431-30446450 None:intergenic 55.0%
GGACTCATAACCGGCATGAC+AGG + Chr8:30446436-30446455 MsG0880043777.01.T02:CDS 55.0%
! CATCATAGTGCTGCTGGTGG+TGG + Chr8:30446703-30446722 MsG0880043777.01.T02:CDS 55.0%
ACGCAGAACACTGCTGGTGG+TGG + Chr8:30446496-30446515 MsG0880043777.01.T02:CDS 60.0%
ATCGCAGCAGCAGCTACTGC+CGG + Chr8:30446409-30446428 MsG0880043777.01.T02:CDS 60.0%
CACACGCAGAACACTGCTGG+TGG + Chr8:30446493-30446512 MsG0880043777.01.T02:CDS 60.0%
CACCACACGCAGAACACTGC+TGG + Chr8:30446490-30446509 MsG0880043777.01.T02:CDS 60.0%
CACCAGCAGTGTTCTGCGTG+TGG - Chr8:30446495-30446514 None:intergenic 60.0%
CAGCAGTGTTCTGCGTGTGG+TGG - Chr8:30446492-30446511 None:intergenic 60.0%
!! CATAGTGCTGCTGGTGGTGG+TGG + Chr8:30446706-30446725 MsG0880043777.01.T02:CDS 60.0%
GAATCTGTCACGGCCGCTGC+AGG + Chr8:30446460-30446479 MsG0880043777.01.T02:CDS 65.0%
GCAGCAGCTACTGCCGGTCA+TGG + Chr8:30446415-30446434 MsG0880043777.01.T02:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 30446067 30446759 30446067 ID=MsG0880043777.01;Name=MsG0880043777.01
Chr8 mRNA 30446067 30446759 30446067 ID=MsG0880043777.01.T01;Parent=MsG0880043777.01;Name=MsG0880043777.01.T01;_AED=0.19;_eAED=0.20;_QI=0|0|0|1|0|0|2|0|220
Chr8 exon 30446067 30446413 30446067 ID=MsG0880043777.01.T01:exon:7266;Parent=MsG0880043777.01.T01
Chr8 exon 30446444 30446759 30446444 ID=MsG0880043777.01.T01:exon:7267;Parent=MsG0880043777.01.T01
Chr8 CDS 30446067 30446413 30446067 ID=MsG0880043777.01.T01:cds;Parent=MsG0880043777.01.T01
Chr8 CDS 30446444 30446759 30446444 ID=MsG0880043777.01.T01:cds;Parent=MsG0880043777.01.T01
Chr8 mRNA 30446067 30446759 30446067 ID=MsG0880043777.01.T02;Parent=MsG0880043777.01;Name=MsG0880043777.01.T02;_AED=0.18;_eAED=0.18;_QI=0|-1|0|1|-1|1|1|0|230
Chr8 exon 30446067 30446759 30446067 ID=MsG0880043777.01.T02:exon:7268;Parent=MsG0880043777.01.T02
Chr8 CDS 30446067 30446759 30446067 ID=MsG0880043777.01.T02:cds;Parent=MsG0880043777.01.T02
Gene Sequence

>MsG0880043777.01.T01

ATGGCATCCTCAAATTCACCATGTGCAGCATGCAAGTTTCTACGCCGCAAATGCCAACCAGAATGTGCATTTGCACCTTATTTTCCACCTGATCAGCCACAAAAATTTGCAAATGTTCATAGAATTTTTGGAGCAAGCAATGTTACAAAGCTTCTCAATGATTTACACCCTCACCAACGTGAAGATGCTGTTAATTCACTTGCTTATGAAGCTGAGATGCGACTTCGTGATCCTGTTTATGGTTGTGTTGGTGTAATTTCTCTCCTTCAACACCAACTTCGTCAGCTTCAAATGGATCTTTATTGTGCTAAATCCGAACTTTCTCGGTTTCAAAACTTGAGCATCGCAACCGGCATGACAGGTGAATCTGTCACGGCCGCTGCAGGTAATTACCACCACACGCAGAACACTGCTGGTGGTGGTAATAACACTAACGGCCGTGACCATCGTTATCATCATCATCAACATCATCAGTTTTTTCCAAGGGATCAACATCAACAACATAATTTGGTAAGAAATTTTGATGGTGGAATTGGGAACAACTATGATGCTAGTCTCTTGGCTATGAATATTTCTGCTAGCTTAGGACAGATCAATCAACTTCAACATCATAGTGCTGCTGGTGGTGGTGGTGACGACCGCCGTACTGTTAACCGCTCCTAG

>MsG0880043777.01.T02

ATGGCATCCTCAAATTCACCATGTGCAGCATGCAAGTTTCTACGCCGCAAATGCCAACCAGAATGTGCATTTGCACCTTATTTTCCACCTGATCAGCCACAAAAATTTGCAAATGTTCATAGAATTTTTGGAGCAAGCAATGTTACAAAGCTTCTCAATGATTTACACCCTCACCAACGTGAAGATGCTGTTAATTCACTTGCTTATGAAGCTGAGATGCGACTTCGTGATCCTGTTTATGGTTGTGTTGGTGTAATTTCTCTCCTTCAACACCAACTTCGTCAGCTTCAAATGGATCTTTATTGTGCTAAATCCGAACTTTCTCGGTTTCAAAACTTGAGCATCGCAGCAGCAGCTACTGCCGGTCATGGACTCATAACCGGCATGACAGGTGAATCTGTCACGGCCGCTGCAGGTAATTACCACCACACGCAGAACACTGCTGGTGGTGGTAATAACACTAACGGCCGTGACCATCGTTATCATCATCATCAACATCATCAGTTTTTTCCAAGGGATCAACATCAACAACATAATTTGGTAAGAAATTTTGATGGTGGAATTGGGAACAACTATGATGCTAGTCTCTTGGCTATGAATATTTCTGCTAGCTTAGGACAGATCAATCAACTTCAACATCATAGTGCTGCTGGTGGTGGTGGTGACGACCGCCGTACTGTTAACCGCTCCTAG
Protein sequence

>MsG0880043777.01.T01

MASSNSPCAACKFLRRKCQPECAFAPYFPPDQPQKFANVHRIFGASNVTKLLNDLHPHQREDAVNSLAYEAEMRLRDPVYGCVGVISLLQHQLRQLQMDLYCAKSELSRFQNLSIATGMTGESVTAAAGNYHHTQNTAGGGNNTNGRDHRYHHHQHHQFFPRDQHQQHNLVRNFDGGIGNNYDASLLAMNISASLGQINQLQHHSAAGGGGDDRRTVNRS*

>MsG0880043777.01.T02

MASSNSPCAACKFLRRKCQPECAFAPYFPPDQPQKFANVHRIFGASNVTKLLNDLHPHQREDAVNSLAYEAEMRLRDPVYGCVGVISLLQHQLRQLQMDLYCAKSELSRFQNLSIAAAATAGHGLITGMTGESVTAAAGNYHHTQNTAGGGNNTNGRDHRYHHHQHHQFFPRDQHQQHNLVRNFDGGIGNNYDASLLAMNISASLGQINQLQHHSAAGGGGDDRRTVNRS*