Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044188.01.T01 | XP_013465623.1 | 61.538 | 91 | 34 | 1 | 51 | 140 | 4 | 94 | 2.82E-33 | 125 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044188.01.T01 | Q9LMM8 | 53.061 | 49 | 23 | 0 | 75 | 123 | 24 | 72 | 4.99E-12 | 64.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044188.01.T01 | A0A072VC91 | 61.538 | 91 | 34 | 1 | 51 | 140 | 4 | 94 | 1.35e-33 | 125 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0880044188.01.T01 | TF | MADS-M-type |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044188.01.T01 | MTR_2g105290 | 61.538 | 91 | 34 | 1 | 51 | 140 | 4 | 94 | 3.42e-37 | 125 |
| MsG0880044188.01.T01 | MTR_4g127140 | 72.857 | 70 | 19 | 0 | 72 | 141 | 26 | 95 | 7.24e-34 | 117 |
| MsG0880044188.01.T01 | MTR_8g086290 | 50.000 | 88 | 41 | 2 | 55 | 140 | 8 | 94 | 5.71e-26 | 96.7 |
| MsG0880044188.01.T01 | MTR_7g028448 | 60.938 | 64 | 25 | 0 | 75 | 138 | 29 | 92 | 8.19e-26 | 96.3 |
| MsG0880044188.01.T01 | MTR_4g036915 | 60.938 | 64 | 25 | 0 | 75 | 138 | 29 | 92 | 8.19e-26 | 96.3 |
| MsG0880044188.01.T01 | MTR_8g043650 | 34.343 | 99 | 55 | 3 | 51 | 140 | 1 | 98 | 2.80e-16 | 72.8 |
| MsG0880044188.01.T01 | MTR_1g012570 | 34.343 | 99 | 55 | 3 | 51 | 140 | 1 | 98 | 5.98e-16 | 72.0 |
| MsG0880044188.01.T01 | MTR_1g105910 | 33.333 | 99 | 56 | 3 | 51 | 140 | 1 | 98 | 1.30e-15 | 71.2 |
| MsG0880044188.01.T01 | MTR_3g052870 | 32.323 | 99 | 57 | 3 | 51 | 140 | 1 | 98 | 1.70e-15 | 70.5 |
| MsG0880044188.01.T01 | MTR_0121s0080 | 35.366 | 82 | 52 | 1 | 55 | 135 | 5 | 86 | 2.71e-15 | 70.1 |
| MsG0880044188.01.T01 | MTR_1g105920 | 32.323 | 99 | 57 | 3 | 51 | 140 | 1 | 98 | 5.44e-15 | 69.3 |
| MsG0880044188.01.T01 | MTR_3g052920 | 33.333 | 99 | 56 | 3 | 51 | 140 | 1 | 98 | 6.84e-15 | 69.3 |
| MsG0880044188.01.T01 | MTR_1g106070 | 29.592 | 98 | 61 | 2 | 51 | 140 | 1 | 98 | 9.03e-15 | 67.8 |
| MsG0880044188.01.T01 | MTR_5g045560 | 37.179 | 78 | 46 | 2 | 51 | 126 | 1 | 77 | 3.78e-14 | 67.0 |
| MsG0880044188.01.T01 | MTR_4g131030 | 42.623 | 61 | 35 | 0 | 75 | 135 | 19 | 79 | 6.14e-14 | 66.2 |
| MsG0880044188.01.T01 | MTR_1g077360 | 35.897 | 78 | 47 | 2 | 51 | 126 | 1 | 77 | 1.55e-13 | 65.5 |
| MsG0880044188.01.T01 | MTR_1g047550 | 39.062 | 64 | 39 | 0 | 75 | 138 | 19 | 82 | 1.67e-13 | 64.3 |
| MsG0880044188.01.T01 | MTR_1g105905 | 40.000 | 65 | 32 | 1 | 75 | 132 | 9 | 73 | 2.03e-13 | 65.1 |
| MsG0880044188.01.T01 | MTR_8g079502 | 41.935 | 62 | 36 | 0 | 75 | 136 | 19 | 80 | 3.01e-13 | 63.9 |
| MsG0880044188.01.T01 | MTR_3g080940 | 32.468 | 77 | 51 | 1 | 51 | 126 | 1 | 77 | 3.07e-13 | 64.3 |
| MsG0880044188.01.T01 | MTR_1g075600 | 37.179 | 78 | 46 | 2 | 51 | 126 | 1 | 77 | 3.41e-13 | 64.7 |
| MsG0880044188.01.T01 | MTR_2g030740 | 32.967 | 91 | 51 | 3 | 51 | 132 | 1 | 90 | 3.48e-13 | 64.7 |
| MsG0880044188.01.T01 | MTR_3g030770 | 46.154 | 52 | 28 | 0 | 75 | 126 | 26 | 77 | 5.03e-13 | 63.9 |
| MsG0880044188.01.T01 | MTR_1g108510 | 46.154 | 52 | 28 | 0 | 75 | 126 | 26 | 77 | 6.50e-13 | 63.9 |
| MsG0880044188.01.T01 | MTR_3g030780 | 31.646 | 79 | 49 | 2 | 51 | 126 | 1 | 77 | 1.29e-12 | 61.2 |
| MsG0880044188.01.T01 | MTR_5g053390 | 33.766 | 77 | 50 | 1 | 51 | 126 | 1 | 77 | 1.46e-12 | 62.8 |
| MsG0880044188.01.T01 | MTR_7g062350 | 31.818 | 88 | 45 | 1 | 42 | 129 | 20 | 92 | 1.62e-12 | 62.8 |
| MsG0880044188.01.T01 | MTR_2g085280 | 41.333 | 75 | 37 | 3 | 59 | 126 | 3 | 77 | 2.89e-12 | 62.0 |
| MsG0880044188.01.T01 | MTR_2g085250 | 41.333 | 75 | 37 | 3 | 59 | 126 | 3 | 77 | 3.24e-12 | 62.0 |
| MsG0880044188.01.T01 | MTR_8g051580 | 33.333 | 81 | 50 | 1 | 55 | 135 | 3 | 79 | 3.27e-12 | 61.2 |
| MsG0880044188.01.T01 | MTR_1g063160 | 35.714 | 84 | 51 | 2 | 58 | 140 | 1 | 82 | 5.01e-12 | 60.5 |
| MsG0880044188.01.T01 | MTR_1g054265 | 35.616 | 73 | 43 | 1 | 55 | 127 | 3 | 71 | 5.69e-12 | 60.1 |
| MsG0880044188.01.T01 | MTR_5g055100 | 34.615 | 78 | 48 | 2 | 51 | 126 | 1 | 77 | 2.45e-11 | 59.7 |
| MsG0880044188.01.T01 | MTR_2g093190 | 31.169 | 77 | 52 | 1 | 51 | 126 | 1 | 77 | 2.45e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044188.01.T01 | AT1G01530 | 53.061 | 49 | 23 | 0 | 75 | 123 | 24 | 72 | 5.09e-13 | 64.3 |
| MsG0880044188.01.T01 | AT1G65360 | 38.356 | 73 | 42 | 2 | 55 | 125 | 3 | 74 | 6.19e-13 | 63.9 |
| MsG0880044188.01.T01 | AT5G60440 | 45.763 | 59 | 30 | 1 | 70 | 126 | 17 | 75 | 1.17e-12 | 63.9 |
| MsG0880044188.01.T01 | AT2G24840 | 46.154 | 52 | 28 | 0 | 75 | 126 | 80 | 131 | 1.42e-11 | 60.5 |
| MsG0880044188.01.T01 | AT3G66656 | 39.394 | 66 | 36 | 2 | 64 | 125 | 4 | 69 | 2.62e-11 | 58.5 |
Find 29 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCCCATGTTCTTCTTTCTT+TGG | 0.236667 | 8:-37281540 | None:intergenic |
| GGTGACAAACTCTATTGTTT+TGG | 0.315998 | 8:+37281704 | MsG0880044188.01.T01:CDS |
| CCTCTTAAGACTTGTCTTGC+TGG | 0.324065 | 8:-37280836 | None:intergenic |
| CCACAGAGTAATGGTATTAG+AGG | 0.428190 | 8:-37280860 | None:intergenic |
| CAAAGGAACCACTGAGTTTG+AGG | 0.435112 | 8:+37281763 | MsG0880044188.01.T01:CDS |
| TTCCTTTGATGTAGCTATCA+AGG | 0.460132 | 8:-37281748 | None:intergenic |
| TGAACATGGTGAAGCTGGTC+AGG | 0.478697 | 8:+37280887 | MsG0880044188.01.T01:CDS |
| TTAAGACTTGTCTTGCTGGT+TGG | 0.487029 | 8:-37280832 | None:intergenic |
| GCAAATGTCTTCAGATGTAC+AGG | 0.508853 | 8:+37280768 | None:intergenic |
| GCTGTTGAACATGGTGAAGC+TGG | 0.517398 | 8:+37280882 | MsG0880044188.01.T01:CDS |
| CCAGCAAGACAAGTCTTAAG+AGG | 0.529738 | 8:+37280836 | MsG0880044188.01.T01:CDS |
| CCTTCTTGATCTCAATCTTG+TGG | 0.542793 | 8:-37281564 | None:intergenic |
| TCAAGGACAACGTTAGCGTC+GGG | 0.552541 | 8:-37281731 | None:intergenic |
| ATCAAGGACAACGTTAGCGT+CGG | 0.568754 | 8:-37281732 | None:intergenic |
| TGGTGAAGCTGGTCAGGCAT+CGG | 0.570111 | 8:+37280893 | MsG0880044188.01.T01:CDS |
| TCACTTTCTCAAAGAAGACA+AGG | 0.572697 | 8:+37281611 | MsG0880044188.01.T01:CDS |
| TACTCTGTGGCTGTTGAACA+TGG | 0.576799 | 8:+37280873 | MsG0880044188.01.T01:CDS |
| GTTCAACAGCCACAGAGTAA+TGG | 0.588765 | 8:-37280869 | None:intergenic |
| CTTTGAATCCTCAAACTCAG+TGG | 0.591334 | 8:-37281771 | None:intergenic |
| GTCCTTGATAGCTACATCAA+AGG | 0.593323 | 8:+37281746 | MsG0880044188.01.T01:CDS |
| AACCAAAGAAAGAAGAACAT+GGG | 0.601697 | 8:+37281538 | MsG0880044188.01.T01:CDS |
| AACAATAGAGTTTGTCACCA+GGG | 0.604022 | 8:-37281700 | None:intergenic |
| CCACAAGATTGAGATCAAGA+AGG | 0.650458 | 8:+37281564 | MsG0880044188.01.T01:CDS |
| GGTTGGTGTGTCAATCATAG+AGG | 0.650861 | 8:-37280815 | None:intergenic |
| TAACCAAAGAAAGAAGAACA+TGG | 0.653064 | 8:+37281537 | MsG0880044188.01.T01:CDS |
| AAACAATAGAGTTTGTCACC+AGG | 0.667283 | 8:-37281701 | None:intergenic |
| ACCAAAGAAAGAAGAACATG+GGG | 0.674096 | 8:+37281539 | MsG0880044188.01.T01:CDS |
| AATGTCTTCAGATGTACAGG+AGG | 0.694457 | 8:+37280771 | None:intergenic |
| CCTCTAATACCATTACTCTG+TGG | 0.719923 | 8:+37280860 | MsG0880044188.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATCTAAATATTTTTTTTGAA+GGG | + | Chr8:37281263-37281282 | MsG0880044188.01.T01:intron | 10.0% |
| !!! | TAAATAAAAGAATTTTAAGA+AGG | - | Chr8:37281332-37281351 | None:intergenic | 10.0% |
| !! | AAAACAGTTTGATAAAAAAA+TGG | + | Chr8:37281360-37281379 | MsG0880044188.01.T01:intron | 15.0% |
| !! | TCAAAAAAAATATTTAGATG+AGG | - | Chr8:37281262-37281281 | None:intergenic | 15.0% |
| !!! | ATGTAAGAGTTAATAATTTT+TGG | + | Chr8:37281403-37281422 | MsG0880044188.01.T01:intron | 15.0% |
| !!! | CATCTAAATATTTTTTTTGA+AGG | + | Chr8:37281262-37281281 | MsG0880044188.01.T01:intron | 15.0% |
| !!! | TATACTATTTTTTTGATTCA+AGG | + | Chr8:37281145-37281164 | MsG0880044188.01.T01:intron | 15.0% |
| !!! | TTATCAAACTGTTTTAAAGA+CGG | - | Chr8:37281355-37281374 | None:intergenic | 20.0% |
| ! | GTCATGGAAATTGTTTAATA+TGG | + | Chr8:37281081-37281100 | MsG0880044188.01.T01:intron | 25.0% |
| !! | ATTGATCTAGAATCTTAGAT+AGG | + | Chr8:37281452-37281471 | MsG0880044188.01.T01:intron | 25.0% |
| !! | TGTTTTTCCTTTATGTAACA+GGG | + | Chr8:37281053-37281072 | MsG0880044188.01.T01:intron | 25.0% |
| !! | TTGATCTAGAATCTTAGATA+GGG | + | Chr8:37281453-37281472 | MsG0880044188.01.T01:intron | 25.0% |
| !!! | TATTTAGATGAGGTTTTGTT+TGG | - | Chr8:37281252-37281271 | None:intergenic | 25.0% |
| AACCAAAGAAAGAAGAACAT+GGG | + | Chr8:37281538-37281557 | MsG0880044188.01.T01:CDS | 30.0% | |
| ATGGTACATTAAAACGTTTG+TGG | + | Chr8:37281100-37281119 | MsG0880044188.01.T01:intron | 30.0% | |
| TAACCAAAGAAAGAAGAACA+TGG | + | Chr8:37281537-37281556 | MsG0880044188.01.T01:CDS | 30.0% | |
| ! | GTGTTTTTCCTTTATGTAAC+AGG | + | Chr8:37281052-37281071 | MsG0880044188.01.T01:intron | 30.0% |
| AAACAATAGAGTTTGTCACC+AGG | - | Chr8:37281704-37281723 | None:intergenic | 35.0% | |
| AACAATAGAGTTTGTCACCA+GGG | - | Chr8:37281703-37281722 | None:intergenic | 35.0% | |
| AATCCTAAACGTCTGATTCA+TGG | - | Chr8:37281030-37281049 | None:intergenic | 35.0% | |
| ACCAAAGAAAGAAGAACATG+GGG | + | Chr8:37281539-37281558 | MsG0880044188.01.T01:CDS | 35.0% | |
| ATCGGTTACAAAACCTAACA+GGG | + | Chr8:37280911-37280930 | MsG0880044188.01.T01:intron | 35.0% | |
| CTTAGATAGGGAGAAATTGT+AGG | + | Chr8:37281465-37281484 | MsG0880044188.01.T01:intron | 35.0% | |
| TCACTTTCTCAAAGAAGACA+AGG | + | Chr8:37281611-37281630 | MsG0880044188.01.T01:CDS | 35.0% | |
| TTCCTTTGATGTAGCTATCA+AGG | - | Chr8:37281751-37281770 | None:intergenic | 35.0% | |
| TTTGGTTAACCATATCCTGA+TGG | - | Chr8:37281525-37281544 | None:intergenic | 35.0% | |
| ! | CAAGTAAACAGCTTTAGTGT+CGG | - | Chr8:37280961-37280980 | None:intergenic | 35.0% |
| ! | GGTGACAAACTCTATTGTTT+TGG | + | Chr8:37281704-37281723 | MsG0880044188.01.T01:CDS | 35.0% |
| ACATCGACCCTGTTACATAA+AGG | - | Chr8:37281063-37281082 | None:intergenic | 40.0% | |
| CCACAAGATTGAGATCAAGA+AGG | + | Chr8:37281564-37281583 | MsG0880044188.01.T01:CDS | 40.0% | |
| CCACAGAGTAATGGTATTAG+AGG | - | Chr8:37280863-37280882 | None:intergenic | 40.0% | |
| CCTCTAATACCATTACTCTG+TGG | + | Chr8:37280860-37280879 | MsG0880044188.01.T01:CDS | 40.0% | |
| CCTTCTTGATCTCAATCTTG+TGG | - | Chr8:37281567-37281586 | None:intergenic | 40.0% | |
| CTTTGAATCCTCAAACTCAG+TGG | - | Chr8:37281774-37281793 | None:intergenic | 40.0% | |
| GAGAATGCAATTTCACCATC+AGG | + | Chr8:37281507-37281526 | MsG0880044188.01.T01:intron | 40.0% | |
| GCAATTTCACCATCAGGATA+TGG | + | Chr8:37281513-37281532 | MsG0880044188.01.T01:intron | 40.0% | |
| TCTCCATGAATCAGACGTTT+AGG | + | Chr8:37281024-37281043 | MsG0880044188.01.T01:intron | 40.0% | |
| TTAAGACTTGTCTTGCTGGT+TGG | - | Chr8:37280835-37280854 | None:intergenic | 40.0% | |
| TTGACACAAACAACCCTGTT+AGG | - | Chr8:37280927-37280946 | None:intergenic | 40.0% | |
| ! | CATCGGTTACAAAACCTAAC+AGG | + | Chr8:37280910-37280929 | MsG0880044188.01.T01:intron | 40.0% |
| ! | GTCCTTGATAGCTACATCAA+AGG | + | Chr8:37281746-37281765 | MsG0880044188.01.T01:CDS | 40.0% |
| !! | TAAACAGCTTTAGTGTCGGT+AGG | - | Chr8:37280957-37280976 | None:intergenic | 40.0% |
| ACCAGGGAAAAACGCAATGA+CGG | - | Chr8:37281687-37281706 | None:intergenic | 45.0% | |
| ATCAAGGACAACGTTAGCGT+CGG | - | Chr8:37281735-37281754 | None:intergenic | 45.0% | |
| ATGTAACAGGGTCGATGTCA+TGG | + | Chr8:37281065-37281084 | MsG0880044188.01.T01:intron | 45.0% | |
| CAAAGGAACCACTGAGTTTG+AGG | + | Chr8:37281763-37281782 | MsG0880044188.01.T01:CDS | 45.0% | |
| CCAGCAAGACAAGTCTTAAG+AGG | + | Chr8:37280836-37280855 | MsG0880044188.01.T01:CDS | 45.0% | |
| CCTCTTAAGACTTGTCTTGC+TGG | - | Chr8:37280839-37280858 | None:intergenic | 45.0% | |
| CTGTTTACTTGCTCGAATGC+TGG | + | Chr8:37280970-37280989 | MsG0880044188.01.T01:intron | 45.0% | |
| GCCCCATGTTCTTCTTTCTT+TGG | - | Chr8:37281543-37281562 | None:intergenic | 45.0% | |
| GTTCAACAGCCACAGAGTAA+TGG | - | Chr8:37280872-37280891 | None:intergenic | 45.0% | |
| TACTCTGTGGCTGTTGAACA+TGG | + | Chr8:37280873-37280892 | MsG0880044188.01.T01:CDS | 45.0% | |
| ! | GGTTGGTGTGTCAATCATAG+AGG | - | Chr8:37280818-37280837 | None:intergenic | 45.0% |
| GCTGTTGAACATGGTGAAGC+TGG | + | Chr8:37280882-37280901 | MsG0880044188.01.T01:CDS | 50.0% | |
| TCAAGGACAACGTTAGCGTC+GGG | - | Chr8:37281734-37281753 | None:intergenic | 50.0% | |
| TGAACATGGTGAAGCTGGTC+AGG | + | Chr8:37280887-37280906 | MsG0880044188.01.T01:CDS | 50.0% | |
| GTATGACCCGTGCGTTAGCA+CGG | + | Chr8:37281206-37281225 | MsG0880044188.01.T01:intron | 55.0% | |
| TATGACCCGTGCGTTAGCAC+GGG | + | Chr8:37281207-37281226 | MsG0880044188.01.T01:intron | 55.0% | |
| TGGTGAAGCTGGTCAGGCAT+CGG | + | Chr8:37280893-37280912 | MsG0880044188.01.T01:CDS | 55.0% | |
| ! | GCCGTCATTGCGTTTTTCCC+TGG | + | Chr8:37281683-37281702 | MsG0880044188.01.T01:CDS | 55.0% |
| ATCGACCCGTGCTAACGCAC+GGG | - | Chr8:37281215-37281234 | None:intergenic | 60.0% | |
| CATCGACCCGTGCTAACGCA+CGG | - | Chr8:37281216-37281235 | None:intergenic | 60.0% | |
| CGTGCGTTAGCACGGGTCGA+TGG | + | Chr8:37281214-37281233 | MsG0880044188.01.T01:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 37280772 | 37281811 | 37280772 | ID=MsG0880044188.01;Name=MsG0880044188.01 |
| Chr8 | mRNA | 37280772 | 37281811 | 37280772 | ID=MsG0880044188.01.T01;Parent=MsG0880044188.01;Name=MsG0880044188.01.T01;_AED=0.21;_eAED=0.29;_QI=0|0|0|1|1|1|2|0|141 |
| Chr8 | exon | 37280772 | 37280914 | 37280772 | ID=MsG0880044188.01.T01:exon:3012;Parent=MsG0880044188.01.T01 |
| Chr8 | exon | 37281529 | 37281811 | 37281529 | ID=MsG0880044188.01.T01:exon:3011;Parent=MsG0880044188.01.T01 |
| Chr8 | CDS | 37280772 | 37280914 | 37280772 | ID=MsG0880044188.01.T01:cds;Parent=MsG0880044188.01.T01 |
| Chr8 | CDS | 37281529 | 37281811 | 37281529 | ID=MsG0880044188.01.T01:cds;Parent=MsG0880044188.01.T01 |
| Gene Sequence |
| Protein sequence |
