Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044190.01.T01 | XP_003628271.1 | 94.475 | 181 | 10 | 0 | 1 | 181 | 1 | 181 | 2.65E-120 | 347 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044190.01.T01 | MTR_8g051580 | 94.475 | 181 | 10 | 0 | 1 | 181 | 1 | 181 | 3.21e-124 | 347 |
| MsG0880044190.01.T01 | MTR_1g047550 | 59.429 | 175 | 71 | 0 | 1 | 175 | 1 | 175 | 1.18e-72 | 217 |
| MsG0880044190.01.T01 | MTR_8g046350 | 63.314 | 169 | 56 | 2 | 1 | 169 | 1 | 163 | 3.12e-69 | 207 |
| MsG0880044190.01.T01 | MTR_1g063160 | 56.000 | 175 | 77 | 0 | 1 | 175 | 1 | 175 | 1.91e-68 | 206 |
| MsG0880044190.01.T01 | MTR_1g054265 | 58.333 | 168 | 70 | 0 | 1 | 168 | 1 | 168 | 9.29e-67 | 201 |
| MsG0880044190.01.T01 | MTR_8g079502 | 57.831 | 166 | 70 | 0 | 1 | 166 | 1 | 166 | 8.32e-66 | 199 |
| MsG0880044190.01.T01 | MTR_4g131030 | 52.941 | 187 | 71 | 1 | 1 | 170 | 1 | 187 | 9.23e-65 | 198 |
| MsG0880044190.01.T01 | MTR_4g094632 | 39.881 | 168 | 81 | 5 | 1 | 164 | 1 | 152 | 6.24e-29 | 105 |
| MsG0880044190.01.T01 | MTR_3g080940 | 43.902 | 123 | 59 | 2 | 1 | 117 | 8 | 126 | 4.58e-25 | 96.7 |
| MsG0880044190.01.T01 | MTR_1g106070 | 46.535 | 101 | 52 | 1 | 2 | 100 | 9 | 109 | 1.21e-24 | 95.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044190.01.T01 | AT2G34440 | 42.197 | 173 | 88 | 4 | 1 | 162 | 1 | 172 | 5.06e-33 | 116 |
| MsG0880044190.01.T01 | AT3G66656 | 40.341 | 176 | 88 | 4 | 1 | 160 | 1 | 175 | 1.32e-30 | 110 |
| MsG0880044190.01.T01 | AT2G24840 | 37.805 | 164 | 87 | 4 | 1 | 150 | 62 | 224 | 3.02e-30 | 111 |
| MsG0880044190.01.T01 | AT5G60440 | 43.558 | 163 | 65 | 6 | 2 | 143 | 7 | 163 | 2.10e-27 | 105 |
| MsG0880044190.01.T01 | AT1G01530 | 46.018 | 113 | 58 | 3 | 1 | 112 | 6 | 116 | 8.92e-23 | 91.7 |
| MsG0880044190.01.T01 | AT1G47760 | 55.882 | 68 | 30 | 0 | 1 | 68 | 1 | 68 | 3.68e-22 | 88.6 |
| MsG0880044190.01.T01 | AT4G36590 | 57.353 | 68 | 29 | 0 | 2 | 69 | 7 | 74 | 1.20e-20 | 86.3 |
| MsG0880044190.01.T01 | AT3G57230 | 47.674 | 86 | 38 | 1 | 1 | 86 | 1 | 79 | 1.37e-20 | 83.6 |
| MsG0880044190.01.T01 | AT3G57230 | 47.674 | 86 | 38 | 1 | 1 | 86 | 1 | 79 | 3.70e-20 | 84.7 |
| MsG0880044190.01.T01 | AT3G57230 | 47.674 | 86 | 38 | 1 | 1 | 86 | 1 | 79 | 3.70e-20 | 84.7 |
Find 43 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAGTAAGGCTTATTCCC+AGG | 0.528202 | 8:+37324079 | None:intergenic |
| AACAAAGTCGCAAGAAAGAA+AGG | 0.460568 | 8:-37323722 | MsG0880044190.01.T01:CDS |
| AACTGAACTCACTCAACTTG+AGG | 0.538837 | 8:-37323858 | MsG0880044190.01.T01:CDS |
| AAGAGTAAGGCTTATTCCCA+GGG | 0.598555 | 8:+37324080 | None:intergenic |
| AATGAACTTGCCATCTTGTG+CGG | 0.538350 | 8:-37324130 | MsG0880044190.01.T01:CDS |
| AATGTAACCTGCCTTATGAT+AGG | 0.459002 | 8:+37324187 | None:intergenic |
| AATTTCTGCCCCGCACAAGA+TGG | 0.555860 | 8:+37324120 | None:intergenic |
| ACATTCTCTAAGCGTCGAAC+AGG | 0.429261 | 8:-37324169 | MsG0880044190.01.T01:CDS |
| ACTTCTTGCAGAGAAACCGG+TGG | 0.709944 | 8:-37323756 | MsG0880044190.01.T01:CDS |
| AGAGTAAGGCTTATTCCCAG+GGG | 0.760036 | 8:+37324081 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGATTCATATCGGAAATTGA+AGG | - | Chr8:37324101-37324120 | MsG0880044190.01.T01:CDS | 30.0% | |
| ATATCGGAAATTGAAGGAAA+TGG | - | Chr8:37324107-37324126 | MsG0880044190.01.T01:CDS | 30.0% | |
| AACAAAGTCGCAAGAAAGAA+AGG | - | Chr8:37324207-37324226 | MsG0880044190.01.T01:CDS | 35.0% | |
| AATGTAACCTGCCTTATGAT+AGG | + | Chr8:37323745-37323764 | None:intergenic | 35.0% | |
| ATGCAACATCATCAACACTT+GGG | + | Chr8:37323882-37323901 | None:intergenic | 35.0% | |
| ATGTAACCTGCCTTATGATA+GGG | + | Chr8:37323744-37323763 | None:intergenic | 35.0% | |
| GTTGCATCCAAATTTCTTGA+AGG | - | Chr8:37323895-37323914 | MsG0880044190.01.T01:CDS | 35.0% | |
| TCGAACAGGATTATTCAAGA+AGG | - | Chr8:37323774-37323793 | MsG0880044190.01.T01:CDS | 35.0% | |
| AAAGAGTAAGGCTTATTCCC+AGG | + | Chr8:37323853-37323872 | None:intergenic | 40.0% | |
| AACTGAACTCACTCAACTTG+AGG | - | Chr8:37324071-37324090 | MsG0880044190.01.T01:CDS | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 37323703 | 37324248 | 37323703 | ID=MsG0880044190.01;Name=MsG0880044190.01 |
| Chr8 | mRNA | 37323703 | 37324248 | 37323703 | ID=MsG0880044190.01.T01;Parent=MsG0880044190.01;Name=MsG0880044190.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|181 |
| Chr8 | exon | 37323703 | 37324248 | 37323703 | ID=MsG0880044190.01.T01:exon:2976;Parent=MsG0880044190.01.T01 |
| Chr8 | CDS | 37323703 | 37324248 | 37323703 | ID=MsG0880044190.01.T01:cds;Parent=MsG0880044190.01.T01 |
| Gene Sequence |
>MsG0880044190.01.T01 ATGGGTCGTCGCAAAATTGCGATTGCGAGGGTGAGAGACCCTATCATAAGGCAGGTTACATTCTCTAAGCGTCGAACAGGATTATTCAAGAAGGCAAATGAACTTGCCATCTTGTGCGGGGCAGAAATTGCTATTGTTGTGTTCTCCCCTGGGAATAAGCCTTACTCTTTCGGGCACCCAAGTGTTGATGATGTTGCATCCAAATTTCTTGAAGGAGAGCTCAACCTAGATGATGGCATAGGAAGCTCATCTTCTGAAGTTCCAAACAGAGAGGATCTGAATCAACAACTTGAAGATGTGCTAGCTCAACTAAAGGAGGCTGAAAATGAAGCTAGGGCACATGATGAGATCCTAGAAGAATACAAACCAACTGAACTCACTCAACTTGAGGATTTGAAAGATTCATATCGGAAATTGAAGGAAATGGTGAGATCACGACTTAGTGATTTAGAAACAACTGAATCTATGTTACTTCTTGCAGAGAAACCGGTGGTTGGAACAAAAAACAAAGTCGCAAGAAAGAAAGGAAAACAACCAATGCTTTAG |
| Protein sequence |
>MsG0880044190.01.T01 MGRRKIAIARVRDPIIRQVTFSKRRTGLFKKANELAILCGAEIAIVVFSPGNKPYSFGHPSVDDVASKFLEGELNLDDGIGSSSSEVPNREDLNQQLEDVLAQLKEAENEARAHDEILEEYKPTELTQLEDLKDSYRKLKEMVRSRLSDLETTESMLLLAEKPVVGTKNKVARKKGKQPML* |
