AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880044452.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880044452.01.T01 MTR_4g051638 78.395 162 29 1 24 185 80 235 1.57e-83 247
MsG0880044452.01.T01 MTR_7g050385 81.208 149 28 0 37 185 78 226 1.96e-82 244
MsG0880044452.01.T01 MTR_3g072200 77.301 163 25 2 23 185 7 157 9.28e-78 242
MsG0880044452.01.T01 MTR_3g437000 89.516 124 13 0 39 162 7 130 1.02e-76 226
MsG0880044452.01.T01 MTR_3g015530 69.182 159 47 1 29 185 3 161 2.33e-69 211
MsG0880044452.01.T01 MTR_1g081740 68.452 168 17 2 24 185 7 144 1.23e-68 207
MsG0880044452.01.T01 MTR_1g059880 59.067 193 43 3 3 187 29 193 9.61e-67 203
MsG0880044452.01.T01 MTR_2g026585 66.082 171 22 2 22 186 6 146 2.93e-66 207
MsG0880044452.01.T01 MTR_4g049670 66.258 163 24 2 24 186 2 133 8.33e-65 196
MsG0880044452.01.T01 MTR_2g451070 77.391 115 26 0 24 138 8 122 1.36e-58 179
MsG0880044452.01.T01 MTR_5g071980 88.889 99 11 0 24 122 8 106 3.60e-57 181
MsG0880044452.01.T01 MTR_6g084740 87.912 91 11 0 24 114 8 98 2.72e-51 162
MsG0880044452.01.T01 MTR_6g026910 58.571 140 13 1 24 163 8 102 5.78e-45 149
MsG0880044452.01.T01 MTR_5g060540 56.463 147 39 2 39 185 8 129 9.37e-45 152
MsG0880044452.01.T01 MTR_6g075990 50.292 171 75 3 24 185 20 189 2.81e-42 142
MsG0880044452.01.T01 MTR_3g462670 50.610 164 45 3 24 187 2 129 4.68e-42 141
MsG0880044452.01.T01 MTR_4g119600 48.171 164 75 2 24 178 2 164 5.38e-41 141
MsG0880044452.01.T01 MTR_2g067930 54.286 175 33 2 24 186 2 141 8.71e-41 137
MsG0880044452.01.T01 MTR_5g060510 61.151 139 30 1 42 180 42 156 1.66e-40 135
MsG0880044452.01.T01 MTR_5g031650 49.091 165 71 4 24 185 39 193 2.38e-40 137
MsG0880044452.01.T01 MTR_8g009330 47.059 170 80 5 24 185 25 192 1.66e-39 134
MsG0880044452.01.T01 MTR_4g057975 50.318 157 72 5 24 176 2 156 7.67e-39 132
MsG0880044452.01.T01 MTR_3g030230 81.429 70 13 0 116 185 69 138 5.68e-34 119
MsG0880044452.01.T01 MTR_4g057300 73.529 68 18 0 118 185 1 68 1.87e-32 119
MsG0880044452.01.T01 MTR_2g073060 57.143 98 41 1 42 139 70 166 2.26e-30 109
MsG0880044452.01.T01 MTR_3g077875 44.828 116 61 2 72 185 77 191 1.20e-28 108
MsG0880044452.01.T01 MTR_8g107490 47.934 121 59 2 58 178 27 143 2.49e-28 107
MsG0880044452.01.T01 MTR_6g089230 77.778 63 14 0 123 185 28 90 7.51e-28 104
MsG0880044452.01.T01 MTR_3g067290 44.048 168 73 4 20 185 25 173 5.85e-27 102
MsG0880044452.01.T01 MTR_6g090600 55.556 90 39 1 97 186 7 95 1.14e-26 98.2
MsG0880044452.01.T01 MTR_7g106560 45.625 160 45 4 22 181 6 123 2.36e-26 100
MsG0880044452.01.T01 MTR_8g463940 50.000 138 59 5 24 152 32 168 2.48e-26 99.4
MsG0880044452.01.T01 MTR_8g038805 65.079 63 22 0 116 178 50 112 5.63e-26 97.1
MsG0880044452.01.T01 MTR_3g087440 41.104 163 83 4 35 185 30 191 6.74e-26 99.8
MsG0880044452.01.T01 MTR_3g034840 40.000 155 86 4 25 176 22 172 1.87e-25 98.2
MsG0880044452.01.T01 MTR_0599s0020 40.000 155 86 4 25 176 22 172 1.87e-25 98.2
MsG0880044452.01.T01 MTR_7g057800 55.172 87 38 1 45 131 40 125 1.97e-25 99.0
MsG0880044452.01.T01 MTR_7g057800 47.321 112 53 2 34 140 145 255 2.19e-25 99.0
MsG0880044452.01.T01 MTR_6g037570 46.795 156 69 3 24 166 17 171 2.23e-25 99.0
MsG0880044452.01.T01 MTR_0219s0080 50.427 117 50 3 24 133 2 117 1.15e-24 94.0
MsG0880044452.01.T01 MTR_6g464570 43.220 118 60 4 73 185 14 129 1.31e-23 91.7
MsG0880044452.01.T01 MTR_0340s0010 45.185 135 64 3 24 149 44 177 1.39e-22 89.7
MsG0880044452.01.T01 MTR_0341s0040 43.382 136 67 2 24 150 2 136 1.42e-22 88.6
MsG0880044452.01.T01 MTR_8g103800 37.419 155 71 2 24 176 2 132 1.58e-22 92.0
MsG0880044452.01.T01 MTR_1g058140 62.500 64 24 0 122 185 78 141 1.66e-22 90.9
MsG0880044452.01.T01 MTR_7g064870 36.538 156 76 4 32 185 21 155 4.93e-22 88.2
MsG0880044452.01.T01 MTR_5g079560 38.710 155 82 4 35 177 26 179 6.62e-22 89.4
MsG0880044452.01.T01 MTR_2g096070 47.573 103 48 3 78 178 83 181 5.41e-21 90.1
MsG0880044452.01.T01 MTR_5g046160 40.777 103 59 2 74 176 42 142 6.15e-21 87.4
MsG0880044452.01.T01 MTR_6g019000 44.444 117 55 2 24 131 2 117 6.24e-21 84.0
MsG0880044452.01.T01 MTR_2g030885 45.833 120 56 3 24 135 2 120 5.15e-20 87.0
MsG0880044452.01.T01 MTR_5g006100 37.500 160 77 5 28 185 29 167 5.32e-20 87.0
MsG0880044452.01.T01 MTR_3g110070 47.059 119 53 3 24 133 2 119 7.55e-20 81.6
MsG0880044452.01.T01 MTR_2g067720 50.000 100 46 3 38 135 19 116 2.15e-19 80.1
MsG0880044452.01.T01 MTR_2g026605 42.857 119 58 2 24 133 2 119 5.18e-19 79.3
MsG0880044452.01.T01 MTR_8g467980 54.286 70 31 1 79 148 76 144 6.87e-19 79.3
MsG0880044452.01.T01 MTR_1g098990 33.333 171 73 2 24 185 2 140 1.98e-18 80.9
MsG0880044452.01.T01 MTR_7g060350 40.123 162 65 7 24 176 2 140 3.01e-18 78.6
MsG0880044452.01.T01 MTR_4g051612 45.299 117 60 4 38 151 37 152 1.37e-17 77.4
MsG0880044452.01.T01 MTR_6g029530 36.607 112 63 2 73 177 498 608 2.34e-17 79.7
MsG0880044452.01.T01 MTR_3g045850 38.760 129 67 4 32 150 21 147 6.53e-17 74.3
MsG0880044452.01.T01 MTR_8g445570 68.182 44 14 0 143 186 8 51 6.70e-17 73.2
MsG0880044452.01.T01 MTR_6g018990 34.568 162 65 4 24 176 2 131 2.07e-16 72.4
MsG0880044452.01.T01 MTR_0116s0050 35.338 133 51 2 47 176 9 109 4.45e-16 72.4
MsG0880044452.01.T01 MTR_2g034775 81.818 44 8 0 22 65 35 78 6.89e-16 73.2
MsG0880044452.01.T01 MTR_4g009220 52.564 78 31 1 24 95 25 102 7.35e-16 70.1
MsG0880044452.01.T01 MTR_1g075420 48.684 76 34 2 72 143 38 112 1.73e-15 70.1
MsG0880044452.01.T01 MTR_4g113170 58.333 84 23 2 23 100 7 84 2.17e-15 73.9
MsG0880044452.01.T01 MTR_4g113170 78.378 37 8 0 149 185 72 108 2.69e-12 64.7
MsG0880044452.01.T01 MTR_3g116710 36.111 108 65 2 73 177 78 184 2.44e-15 72.0
MsG0880044452.01.T01 MTR_5g070810 43.373 83 44 2 98 178 14 95 2.73e-15 68.9
MsG0880044452.01.T01 MTR_1g012760 30.882 136 84 3 50 182 23 151 4.63e-15 71.6
MsG0880044452.01.T01 MTR_1g012760 32.031 128 77 3 58 182 20 140 5.62e-15 71.6
MsG0880044452.01.T01 MTR_8g059020 41.071 112 56 2 24 126 93 203 2.21e-14 69.3
MsG0880044452.01.T01 MTR_8g095070 42.623 122 61 3 24 137 2 122 2.27e-14 69.3
MsG0880044452.01.T01 MTR_5g015710 51.220 82 37 2 72 151 75 155 2.34e-14 68.6
MsG0880044452.01.T01 MTR_5g098640 42.982 114 56 4 24 129 2 114 2.52e-14 68.2
MsG0880044452.01.T01 MTR_1g075860 53.247 77 33 2 72 146 48 123 5.55e-14 65.9
MsG0880044452.01.T01 MTR_3g434990 36.765 136 75 3 24 149 16 150 1.42e-13 65.5
MsG0880044452.01.T01 MTR_2g105460 44.944 89 47 1 25 111 40 128 2.55e-13 67.8
MsG0880044452.01.T01 MTR_7g065910 50.000 60 28 1 119 176 34 93 3.60e-13 67.0
MsG0880044452.01.T01 MTR_2g090505 33.333 108 68 2 73 177 83 189 4.60e-13 66.6
MsG0880044452.01.T01 MTR_1g099140 34.314 102 63 2 79 177 89 189 7.80e-13 66.2
MsG0880044452.01.T01 MTR_0156s0040 52.381 63 28 1 118 178 1 63 1.19e-12 63.5
MsG0880044452.01.T01 MTR_6g007833 43.878 98 47 2 24 113 2 99 2.05e-12 63.2
MsG0880044452.01.T01 MTR_8g020433 51.667 60 25 1 133 188 70 129 3.34e-12 64.3
MsG0880044452.01.T01 MTR_2g059900 61.224 49 19 0 45 93 40 88 2.01e-11 58.2
MsG0880044452.01.T01 MTR_7g010970 50.000 56 26 1 123 176 6 61 4.76e-11 60.1
MsG0880044452.01.T01 MTR_5g046145 34.343 99 58 2 79 177 125 216 7.68e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 55 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGAAATTGGCTATTCTTAA+TGG 0.192657 8:-42340799 MsG0880044452.01.T01:CDS
AATGGGAGCTTCAACCTTAA+TGG 0.210277 8:+42341164 None:intergenic
AACGGAAGCTTCGACCTTAA+TGG 0.263775 8:+42341146 None:intergenic
AGCAAATAGGGCTGGATTTA+CGG 0.276740 8:-42340992 MsG0880044452.01.T01:CDS
GGAAGACAATGCTTGGAAAT+TGG 0.295925 8:-42340812 MsG0880044452.01.T01:CDS
GTGTCCGTCGACAAGCAAAT+AGG 0.311465 8:-42341005 MsG0880044452.01.T01:CDS
TAGAGTTGTCGATTCCATTA+AGG 0.313410 8:-42341178 MsG0880044452.01.T01:CDS
ACGGAAGCTTCGACCTTAAT+GGG 0.333221 8:+42341147 None:intergenic
GTCGTCCTCCGTTAATCTTC+CGG 0.345153 8:+42340730 None:intergenic
TAAGGTTGAAGCTCCCATTA+AGG 0.367321 8:-42341160 MsG0880044452.01.T01:CDS
CTTTATCTTTCCATGTGTCT+CGG 0.383207 8:+42341043 None:intergenic
TGATAATGCGGGTGATGATC+TGG 0.387992 8:-42341214 MsG0880044452.01.T01:CDS
CGAAGCTTCCGTTAAATTTG+AGG 0.421823 8:-42341136 MsG0880044452.01.T01:CDS
CCTTCTTGCCGGAAGATTAA+CGG 0.424102 8:-42340738 None:intergenic
TGTAGCAGACACCTTCTTGC+CGG 0.425484 8:-42340749 MsG0880044452.01.T01:CDS
GCTTGAAGCCTCAAATTTAA+CGG 0.428130 8:+42341128 None:intergenic
AGGTGCGTGGATATGTCGTT+AGG 0.439715 8:-42340834 MsG0880044452.01.T01:CDS
TTTATCTTTCCATGTGTCTC+GGG 0.441646 8:+42341044 None:intergenic
ATAGATAATGTTGATAATGC+GGG 0.465987 8:-42341225 MsG0880044452.01.T01:intron
AAGAAATGCTATGTTGGAGT+TGG 0.470876 8:-42340944 MsG0880044452.01.T01:CDS
TGTCCGTCGACAAGCAAATA+GGG 0.477393 8:-42341004 MsG0880044452.01.T01:CDS
ATGGAGACTAAAACCTGTGC+GGG 0.481775 8:-42342537 MsG0880044452.01.T01:CDS
GGAAAAGGATCAAGAAAATG+TGG 0.490323 8:-42340868 MsG0880044452.01.T01:CDS
TTAATGGAATCGACAACTCT+AGG 0.502719 8:+42341180 None:intergenic
CGTCGACAAGCAAATAGGGC+TGG 0.520665 8:-42341000 MsG0880044452.01.T01:CDS
AAAATGTGGGTGTTTGTTCA+AGG 0.526388 8:-42340854 MsG0880044452.01.T01:CDS
CCGTTAATCTTCCGGCAAGA+AGG 0.533693 8:+42340738 None:intergenic
TATAGATAATGTTGATAATG+CGG 0.535260 8:-42341226 MsG0880044452.01.T01:intron
CTACATACGGCACCATCTCA+TGG 0.537001 8:+42340767 None:intergenic
AGCCAAGTGGATCAAGGCAG+TGG 0.549372 8:-42342499 MsG0880044452.01.T01:CDS
AAAGTGAAGCATGAAGGAAA+AGG 0.550727 8:-42340883 MsG0880044452.01.T01:CDS
TCGTTAGGGAAGACAATGCT+TGG 0.556018 8:-42340819 MsG0880044452.01.T01:CDS
GGTGCGTGGATATGTCGTTA+GGG 0.556776 8:-42340833 MsG0880044452.01.T01:CDS
GGATGTAGCCAAGTGGATCA+AGG 0.562133 8:-42342505 MsG0880044452.01.T01:CDS
TGAAGGAAGAAATGCTATGT+TGG 0.568759 8:-42340950 MsG0880044452.01.T01:CDS
CAGCCCTATTTGCTTGTCGA+CGG 0.578070 8:+42341001 None:intergenic
GAAAAGGATCAAGAAAATGT+GGG 0.584787 8:-42340867 MsG0880044452.01.T01:CDS
ATCCACTGCCTTGATCCACT+TGG 0.587950 8:+42342497 None:intergenic
GATAATGCGGGTGATGATCT+GGG 0.590704 8:-42341213 MsG0880044452.01.T01:CDS
TGTTGGAGTTGGTTTGTGAA+CGG 0.593854 8:-42340933 MsG0880044452.01.T01:CDS
ATCATTAGAAGATCTTGTGA+AGG 0.593899 8:-42340967 MsG0880044452.01.T01:CDS
AAGAAGGTGTCTGCTACATA+CGG 0.596498 8:+42340754 None:intergenic
AAAGATAAAGATGAATTGCT+CGG 0.597766 8:-42341030 MsG0880044452.01.T01:CDS
AAGACAAAAGTGAAGCATGA+AGG 0.604812 8:-42340889 MsG0880044452.01.T01:CDS
TCGTGACAACGAAGTGGACA+CGG 0.611013 8:-42341082 MsG0880044452.01.T01:CDS
GTCACGAAGTTTCCACACAT+CGG 0.611386 8:+42341098 None:intergenic
GGGTGTTTGTTCAAGGTGCG+TGG 0.617723 8:-42340847 MsG0880044452.01.T01:CDS
CAAGCGTCAATTCCGATGTG+TGG 0.630528 8:-42341110 MsG0880044452.01.T01:CDS
TGGCGTTCACAACCATGAGA+TGG 0.631982 8:-42340779 MsG0880044452.01.T01:CDS
GAAACTTCGTGACAACGAAG+TGG 0.642243 8:-42341088 MsG0880044452.01.T01:CDS
TGGAGACTAAAACCTGTGCG+GGG 0.661058 8:-42342536 MsG0880044452.01.T01:CDS
TAGGGGCGGATGTAGCCAAG+TGG 0.661830 8:-42342512 MsG0880044452.01.T01:CDS
GGAGACTAAAACCTGTGCGG+GGG 0.683594 8:-42342535 MsG0880044452.01.T01:CDS
GATGGAGACTAAAACCTGTG+CGG 0.717713 8:-42342538 MsG0880044452.01.T01:CDS
GAGTTGGTTTGTGAACGGAG+CGG 0.720933 8:-42340928 MsG0880044452.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATTTTCAAAATTTTCTA+AGG - Chr8:42341902-42341921 MsG0880044452.01.T01:intron 10.0%
!!! AAATTTTCAAAATTTTCTAA+GGG - Chr8:42341903-42341922 MsG0880044452.01.T01:intron 10.0%
!!! TTTTTCTTTTGTTTTTTTTT+TGG - Chr8:42341959-42341978 MsG0880044452.01.T01:intron 10.0%
!! AAAAAATTGTGGAAAAAAAA+TGG + Chr8:42341942-42341961 None:intergenic 15.0%
!! AAACAAAAGAAAAAAAATTG+TGG + Chr8:42341953-42341972 None:intergenic 15.0%
!!! AATTTTCAAAATTTTCTAAG+GGG - Chr8:42341904-42341923 MsG0880044452.01.T01:intron 15.0%
!! AAAAAGAAAAAAAGGATTCT+AGG + Chr8:42340889-42340908 None:intergenic 20.0%
!! GAAAAATCAATCAAATGTAA+TGG + Chr8:42341798-42341817 None:intergenic 20.0%
!!! TATAGATAATGTTGATAATG+CGG - Chr8:42342052-42342071 MsG0880044452.01.T01:intron 20.0%
!!! TTACATTTGATTGATTTTTC+TGG - Chr8:42341798-42341817 MsG0880044452.01.T01:intron 20.0%
! AAAGATAAAGATGAATTGCT+CGG - Chr8:42342248-42342267 MsG0880044452.01.T01:intron 25.0%
! CTTTAAATAAATTGCTTGAG+TGG - Chr8:42341675-42341694 MsG0880044452.01.T01:intron 25.0%
! GAGATGACAAAAAGAAAAAA+AGG + Chr8:42340897-42340916 None:intergenic 25.0%
! TATGGTTTCTATGTTTCAAT+CGG + Chr8:42340952-42340971 None:intergenic 25.0%
!! AATAAATGGACCATTTGTTA+TGG - Chr8:42340849-42340868 MsG0880044452.01.T01:CDS 25.0%
!! TTGAAAATTTTCTAAGTCTC+CGG + Chr8:42341893-42341912 None:intergenic 25.0%
!!! ATAGATAATGTTGATAATGC+GGG - Chr8:42342053-42342072 MsG0880044452.01.T01:intron 25.0%
AAAAGAAATACGATTTGCCA+AGG - Chr8:42341446-42341465 MsG0880044452.01.T01:intron 30.0%
ACAAATGGTCCATTTATTGA+TGG + Chr8:42340847-42340866 None:intergenic 30.0%
ATCATTAGAAGATCTTGTGA+AGG - Chr8:42342311-42342330 MsG0880044452.01.T01:intron 30.0%
ATGGTTTCTATGTTTCAATC+GGG + Chr8:42340951-42340970 None:intergenic 30.0%
CAAATGGTCCATTTATTGAT+GGG + Chr8:42340846-42340865 None:intergenic 30.0%
GAAAAGGATCAAGAAAATGT+GGG - Chr8:42342411-42342430 MsG0880044452.01.T01:intron 30.0%
GCAATCGATTAACAATGATT+TGG + Chr8:42341720-42341739 None:intergenic 30.0%
TGGAAATTGGCTATTCTTAA+TGG - Chr8:42342479-42342498 MsG0880044452.01.T01:intron 30.0%
! ATCGATTAACAATGATTTGG+AGG + Chr8:42341717-42341736 None:intergenic 30.0%
! TTTTTCTTTTTGTCATCTCG+AGG - Chr8:42340898-42340917 MsG0880044452.01.T01:CDS 30.0%
AAAATGTGGGTGTTTGTTCA+AGG - Chr8:42342424-42342443 MsG0880044452.01.T01:intron 35.0%
AAAGTGAAGCATGAAGGAAA+AGG - Chr8:42342395-42342414 MsG0880044452.01.T01:intron 35.0%
AAGAAATGCTATGTTGGAGT+TGG - Chr8:42342334-42342353 MsG0880044452.01.T01:intron 35.0%
AAGAAGAGCAAGACAAGATA+AGG + Chr8:42341658-42341677 None:intergenic 35.0%
AAGACAAAAGTGAAGCATGA+AGG - Chr8:42342389-42342408 MsG0880044452.01.T01:intron 35.0%
CTTTATCTTTCCATGTGTCT+CGG + Chr8:42342238-42342257 None:intergenic 35.0%
GCTTGAAGCCTCAAATTTAA+CGG + Chr8:42342153-42342172 None:intergenic 35.0%
GGAAAAGGATCAAGAAAATG+TGG - Chr8:42342410-42342429 MsG0880044452.01.T01:intron 35.0%
TCTAACCAAGCAGTAAAAAG+AGG - Chr8:42341073-42341092 MsG0880044452.01.T01:CDS 35.0%
TCTTGTGTCTCCATAACAAA+TGG + Chr8:42340862-42340881 None:intergenic 35.0%
TGAAGGAAGAAATGCTATGT+TGG - Chr8:42342328-42342347 MsG0880044452.01.T01:intron 35.0%
TTAATGGAATCGACAACTCT+AGG + Chr8:42342101-42342120 None:intergenic 35.0%
TTTATCTTTCCATGTGTCTC+GGG + Chr8:42342237-42342256 None:intergenic 35.0%
! AAAATTTTCTAAGTCTCCGG+CGG + Chr8:42341890-42341909 None:intergenic 35.0%
! CTTAACCTCTTTTTACTGCT+TGG + Chr8:42341081-42341100 None:intergenic 35.0%
! GCATTGGCATTCTATTAAAG+CGG + Chr8:42341216-42341235 None:intergenic 35.0%
! TGCTTGGTTAGAGTACTTAT+CGG + Chr8:42341065-42341084 None:intergenic 35.0%
! TTAGAGTACTTATCGGTAGT+AGG + Chr8:42341058-42341077 None:intergenic 35.0%
!! TAGAGTTGTCGATTCCATTA+AGG - Chr8:42342100-42342119 MsG0880044452.01.T01:intron 35.0%
!! TCATGCTTCACTTTTGTCTT+CGG + Chr8:42342389-42342408 None:intergenic 35.0%
AATGGGAGCTTCAACCTTAA+TGG + Chr8:42342117-42342136 None:intergenic 40.0%
AGCAAATAGGGCTGGATTTA+CGG - Chr8:42342286-42342305 MsG0880044452.01.T01:intron 40.0%
CAGAAGGATACATCCAAAGA+GGG - Chr8:42341396-42341415 MsG0880044452.01.T01:intron 40.0%
CATTTGTGCTTTCAGCAAGT+AGG + Chr8:42340986-42341005 None:intergenic 40.0%
CGAAGCTTCCGTTAAATTTG+AGG - Chr8:42342142-42342161 MsG0880044452.01.T01:intron 40.0%
CTCTAATCGAGCTGTGAAAT+CGG + Chr8:42341607-42341626 None:intergenic 40.0%
CTCTTTGGATGTATCCTTCT+GGG + Chr8:42341397-42341416 None:intergenic 40.0%
GCATAGTCAGAAAGTAAAGC+AGG + Chr8:42341194-42341213 None:intergenic 40.0%
GGAAGACAATGCTTGGAAAT+TGG - Chr8:42342466-42342485 MsG0880044452.01.T01:intron 40.0%
TAAGGTTGAAGCTCCCATTA+AGG - Chr8:42342118-42342137 MsG0880044452.01.T01:intron 40.0%
TAGCTTTCGCTAATGAGAGA+TGG + Chr8:42341488-42341507 None:intergenic 40.0%
TAGTCAGAAAGTAAAGCAGG+AGG + Chr8:42341191-42341210 None:intergenic 40.0%
TCATCTCCGAAACAAACGAA+AGG - Chr8:42341131-42341150 MsG0880044452.01.T01:CDS 40.0%
TCTTTGGATGTATCCTTCTG+GGG + Chr8:42341396-42341415 None:intergenic 40.0%
TTCAAACGTATCCACGCAAT+TGG - Chr8:42340923-42340942 MsG0880044452.01.T01:CDS 40.0%
TTCGCTAATGAGAGATGGAT+TGG + Chr8:42341483-42341502 None:intergenic 40.0%
TTTCGGAGATGAGATTGCAT+TGG + Chr8:42341123-42341142 None:intergenic 40.0%
! ATTTATTGATGGGCGAACGA+CGG + Chr8:42340836-42340855 None:intergenic 40.0%
! CTACTTGCTGAAAGCACAAA+TGG - Chr8:42340984-42341003 MsG0880044452.01.T01:CDS 40.0%
! CTCTAATAGGGGAAAAGCAT+TGG + Chr8:42341232-42341251 None:intergenic 40.0%
! TATTTTCAACCCGAGACACA+TGG - Chr8:42342225-42342244 MsG0880044452.01.T01:intron 40.0%
! TCGCTAATGAGAGATGGATT+GGG + Chr8:42341482-42341501 None:intergenic 40.0%
! TGCTGAAAGCACAAATGGAA+TGG - Chr8:42340989-42341008 MsG0880044452.01.T01:CDS 40.0%
! TGCTTTTCCCCTATTAGAGA+AGG - Chr8:42341233-42341252 MsG0880044452.01.T01:intron 40.0%
!! AAGAAGGTGTCTGCTACATA+CGG + Chr8:42342527-42342546 None:intergenic 40.0%
!! TGTTGGAGTTGGTTTGTGAA+CGG - Chr8:42342345-42342364 MsG0880044452.01.T01:intron 40.0%
AAAGTAAAGCAGGAGGCAGA+AGG + Chr8:42341184-42341203 None:intergenic 45.0%
AAATCGGTGTGGTGAGGTAT+TGG + Chr8:42341591-42341610 None:intergenic 45.0%
AACGGAAGCTTCGACCTTAA+TGG + Chr8:42342135-42342154 None:intergenic 45.0%
AATCCGGCCTTCTCTAATAG+GGG + Chr8:42341243-42341262 None:intergenic 45.0%
ACGGAAGCTTCGACCTTAAT+GGG + Chr8:42342134-42342153 None:intergenic 45.0%
ATGGAGACTAAAACCTGTGC+GGG - Chr8:42340741-42340760 MsG0880044452.01.T01:CDS 45.0%
CAATCCGGCCTTCTCTAATA+GGG + Chr8:42341244-42341263 None:intergenic 45.0%
CATCCAAAGAGGGATTTCCT+CGG - Chr8:42341406-42341425 MsG0880044452.01.T01:intron 45.0%
CCAGAAGGATACATCCAAAG+AGG - Chr8:42341395-42341414 MsG0880044452.01.T01:intron 45.0%
CCTCTTTGGATGTATCCTTC+TGG + Chr8:42341398-42341417 None:intergenic 45.0%
GAAACTTCGTGACAACGAAG+TGG - Chr8:42342190-42342209 MsG0880044452.01.T01:intron 45.0%
GAGCTTCAGCAAAACTAGGT+AGG + Chr8:42341511-42341530 None:intergenic 45.0%
GATAATGCGGGTGATGATCT+GGG - Chr8:42342065-42342084 MsG0880044452.01.T01:intron 45.0%
GTCACGAAGTTTCCACACAT+CGG + Chr8:42342183-42342202 None:intergenic 45.0%
GTCGTTCGCCCATCAATAAA+TGG - Chr8:42340835-42340854 MsG0880044452.01.T01:CDS 45.0%
TCGTTAGGGAAGACAATGCT+TGG - Chr8:42342459-42342478 MsG0880044452.01.T01:intron 45.0%
TGTACTGTCACTCGTTGTAG+CGG - Chr8:42341827-42341846 MsG0880044452.01.T01:intron 45.0%
TGTCCGTCGACAAGCAAATA+GGG - Chr8:42342274-42342293 MsG0880044452.01.T01:intron 45.0%
TTTCCCCTATTAGAGAAGGC+CGG - Chr8:42341237-42341256 MsG0880044452.01.T01:intron 45.0%
! TGATAATGCGGGTGATGATC+TGG - Chr8:42342064-42342083 MsG0880044452.01.T01:intron 45.0%
! TTTATTGATGGGCGAACGAC+GGG + Chr8:42340835-42340854 None:intergenic 45.0%
!! AAAAGAGCACCGTGCTATAC+TGG + Chr8:42341536-42341555 None:intergenic 45.0%
!! CGCTAATGAGAGATGGATTG+GGG + Chr8:42341481-42341500 None:intergenic 45.0%
!! GCTAATGAGAGATGGATTGG+GGG + Chr8:42341480-42341499 None:intergenic 45.0%
AAGTAGGCGACGCGAATCTA+TGG + Chr8:42340970-42340989 None:intergenic 50.0%
AGAAGGTGGGAAAGGAGAGA+GGG + Chr8:42341167-42341186 None:intergenic 50.0%
AGGTGCGTGGATATGTCGTT+AGG - Chr8:42342444-42342463 MsG0880044452.01.T01:intron 50.0%
ATACGATTTGCCAAGGCGAC+CGG - Chr8:42341453-42341472 MsG0880044452.01.T01:intron 50.0%
ATCCACTGCCTTGATCCACT+TGG + Chr8:42340784-42340803 None:intergenic 50.0%
ATCGAGCTGTGAAATCGGTG+TGG + Chr8:42341602-42341621 None:intergenic 50.0%
ATTAGAGAAGGCCGGATTGC+CGG - Chr8:42341245-42341264 MsG0880044452.01.T01:intron 50.0%
CAAGCGTCAATTCCGATGTG+TGG - Chr8:42342168-42342187 MsG0880044452.01.T01:intron 50.0%
CAGCCCTATTTGCTTGTCGA+CGG + Chr8:42342280-42342299 None:intergenic 50.0%
CGCTCCTATCCAAAATGGGT+CGG + Chr8:42341306-42341325 None:intergenic 50.0%
CGGGCTCCTTTCGTTTGTTT+CGG + Chr8:42341140-42341159 None:intergenic 50.0%
CGGTAGTAGGCTGATTAGTG+AGG + Chr8:42341045-42341064 None:intergenic 50.0%
CTACATACGGCACCATCTCA+TGG + Chr8:42342514-42342533 None:intergenic 50.0%
GAAGCTCTGCCAGTATAGCA+CGG - Chr8:42341524-42341543 MsG0880044452.01.T01:intron 50.0%
GCAATCCGGCCTTCTCTAAT+AGG + Chr8:42341245-42341264 None:intergenic 50.0%
GGATGTAGCCAAGTGGATCA+AGG - Chr8:42340773-42340792 MsG0880044452.01.T01:CDS 50.0%
GGCAGAGCTTCAGCAAAACT+AGG + Chr8:42341515-42341534 None:intergenic 50.0%
GGTGCGTGGATATGTCGTTA+GGG - Chr8:42342445-42342464 MsG0880044452.01.T01:intron 50.0%
GTGTCCGTCGACAAGCAAAT+AGG - Chr8:42342273-42342292 MsG0880044452.01.T01:intron 50.0%
TAAAGCAGGAGGCAGAAGGT+GGG + Chr8:42341180-42341199 None:intergenic 50.0%
TATCCAAAATGGGTCGGCGT+CGG + Chr8:42341300-42341319 None:intergenic 50.0%
TCAATCGGGTACCAATTGCG+TGG + Chr8:42340937-42340956 None:intergenic 50.0%
TCGTGACAACGAAGTGGACA+CGG - Chr8:42342196-42342215 MsG0880044452.01.T01:intron 50.0%
TGGAGACTAAAACCTGTGCG+GGG - Chr8:42340742-42340761 MsG0880044452.01.T01:CDS 50.0%
TGGCGTTCACAACCATGAGA+TGG - Chr8:42342499-42342518 MsG0880044452.01.T01:CDS 50.0%
TGTAGCAGACACCTTCTTGC+CGG - Chr8:42342529-42342548 MsG0880044452.01.T01:CDS 50.0%
! CGACCCATTTTGGATAGGAG+CGG - Chr8:42341304-42341323 MsG0880044452.01.T01:intron 50.0%
! TTATTGATGGGCGAACGACG+GGG + Chr8:42340834-42340853 None:intergenic 50.0%
!! GAGTTGGTTTGTGAACGGAG+CGG - Chr8:42342350-42342369 MsG0880044452.01.T01:intron 50.0%
ACTAAAACCTGTGCGGGGGT+AGG - Chr8:42340747-42340766 MsG0880044452.01.T01:CDS 55.0%
AGCCAAGTGGATCAAGGCAG+TGG - Chr8:42340779-42340798 MsG0880044452.01.T01:CDS 55.0%
AGCGGCAGTTAACTACCGCT+GGG - Chr8:42341845-42341864 MsG0880044452.01.T01:intron 55.0%
ATTGTGAATCCACCACGCGC+GGG - Chr8:42340802-42340821 MsG0880044452.01.T01:CDS 55.0%
CAGAAGGTGGGAAAGGAGAG+AGG + Chr8:42341168-42341187 None:intergenic 55.0%
CCGCCGCTCCTATCCAAAAT+GGG + Chr8:42341310-42341329 None:intergenic 55.0%
CGTCGACAAGCAAATAGGGC+TGG - Chr8:42342278-42342297 MsG0880044452.01.T01:intron 55.0%
CTAAAACCTGTGCGGGGGTA+GGG - Chr8:42340748-42340767 MsG0880044452.01.T01:CDS 55.0%
GATTGTGAATCCACCACGCG+CGG - Chr8:42340801-42340820 MsG0880044452.01.T01:CDS 55.0%
GCGAGCACTATTTAGTGCCG+AGG + Chr8:42341426-42341445 None:intergenic 55.0%
GCTGTGAAATCGGTGTGGTG+AGG + Chr8:42341597-42341616 None:intergenic 55.0%
GGAAAGGAGAGAGGGAAAGC+GGG + Chr8:42341159-42341178 None:intergenic 55.0%
GGAGACTAAAACCTGTGCGG+GGG - Chr8:42340743-42340762 MsG0880044452.01.T01:CDS 55.0%
GGGAAAGGAGAGAGGGAAAG+CGG + Chr8:42341160-42341179 None:intergenic 55.0%
GGGAAGGCTAATGCTGCTCT+TGG - Chr8:42341342-42341361 MsG0880044452.01.T01:intron 55.0%
GGGTGTTTGTTCAAGGTGCG+TGG - Chr8:42342431-42342450 MsG0880044452.01.T01:intron 55.0%
GTAAAGCAGGAGGCAGAAGG+TGG + Chr8:42341181-42341200 None:intergenic 55.0%
GTGCCGAGGAAATCCCTCTT+TGG + Chr8:42341412-42341431 None:intergenic 55.0%
TAAAACCTGTGCGGGGGTAG+GGG - Chr8:42340749-42340768 MsG0880044452.01.T01:CDS 55.0%
TAACAACCGCTCACTCCCAG+CGG + Chr8:42341863-42341882 None:intergenic 55.0%
TAACTACCGCTGGGAGTGAG+CGG - Chr8:42341854-42341873 MsG0880044452.01.T01:intron 55.0%
TAGCGGCAGTTAACTACCGC+TGG - Chr8:42341844-42341863 MsG0880044452.01.T01:intron 55.0%
! CCATTTTGGATAGGAGCGGC+GGG - Chr8:42341308-42341327 MsG0880044452.01.T01:intron 55.0%
! CCCATTTTGGATAGGAGCGG+CGG - Chr8:42341307-42341326 MsG0880044452.01.T01:intron 55.0%
! GACGCCGACCCATTTTGGAT+AGG - Chr8:42341299-42341318 MsG0880044452.01.T01:intron 55.0%
!! TATCCTTCTGGGGCTGATGC+TGG + Chr8:42341386-42341405 None:intergenic 55.0%
!! TGAGAGATGGATTGGGGGTC+CGG + Chr8:42341475-42341494 None:intergenic 55.0%
CAGGAGGCAGAAGGTGGGAA+AGG + Chr8:42341175-42341194 None:intergenic 60.0%
CCCGCCGCTCCTATCCAAAA+TGG + Chr8:42341311-42341330 None:intergenic 60.0%
CTGGCTGCTCCACGGAACTA+GGG - Chr8:42341270-42341289 MsG0880044452.01.T01:intron 60.0%
GAGCGGTTGTTAACTGCCGC+CGG - Chr8:42341871-42341890 MsG0880044452.01.T01:intron 60.0%
TAGGGGCGGATGTAGCCAAG+TGG - Chr8:42340766-42340785 MsG0880044452.01.T01:CDS 60.0%
TGGCTGCTCCACGGAACTAG+GGG - Chr8:42341271-42341290 MsG0880044452.01.T01:intron 60.0%
! AAGCCGACGCCGACCCATTT+TGG - Chr8:42341294-42341313 MsG0880044452.01.T01:intron 60.0%
AGCAGCCAGCTCCGGCAATC+CGG + Chr8:42341259-42341278 None:intergenic 65.0%
GAGCCAGCATCAGCCCCAGA+AGG - Chr8:42341380-42341399 MsG0880044452.01.T01:intron 65.0%
GCTGGCTGCTCCACGGAACT+AGG - Chr8:42341269-42341288 MsG0880044452.01.T01:intron 65.0%
TTCCGTGGAGCAGCCAGCTC+CGG + Chr8:42341267-42341286 None:intergenic 65.0%
! GTCGGCTTCCCCTAGTTCCG+TGG + Chr8:42341282-42341301 None:intergenic 65.0%
!! CAGCATTAGCCTTCCCGCCC+CGG + Chr8:42341338-42341357 None:intergenic 65.0%
AACCTGTGCGGGGGTAGGGG+CGG - Chr8:42340752-42340771 MsG0880044452.01.T01:CDS 70.0%
ACGGGGATTGAACCCGCGCG+TGG + Chr8:42340817-42340836 None:intergenic 70.0%
GAAGGCCGGATTGCCGGAGC+TGG - Chr8:42341251-42341270 MsG0880044452.01.T01:intron 70.0%
GATTGGGGGTCCGGTCGCCT+TGG + Chr8:42341466-42341485 None:intergenic 70.0%
GGGATTGAACCCGCGCGTGG+TGG + Chr8:42340814-42340833 None:intergenic 70.0%
TGCCGGAGCTGGCTGCTCCA+CGG - Chr8:42341262-42341281 MsG0880044452.01.T01:intron 70.0%
!! CATTAGCCTTCCCGCCCCGG+CGG + Chr8:42341335-42341354 None:intergenic 70.0%
ATAGGAGCGGCGGGTCCGCC+GGG - Chr8:42341317-42341336 MsG0880044452.01.T01:intron 75.0%
ATCCGCCCCTACCCCCGCAC+AGG + Chr8:42340757-42340776 None:intergenic 75.0%
GATAGGAGCGGCGGGTCCGC+CGG - Chr8:42341316-42341335 MsG0880044452.01.T01:intron 75.0%
! TAGGAGCGGCGGGTCCGCCG+GGG - Chr8:42341318-42341337 MsG0880044452.01.T01:intron 80.0%
! AGCGGCGGGTCCGCCGGGGC+GGG - Chr8:42341322-42341341 MsG0880044452.01.T01:intron 90.0%
! GAGCGGCGGGTCCGCCGGGG+CGG - Chr8:42341321-42341340 MsG0880044452.01.T01:intron 90.0%
! GCGGGTCCGCCGGGGCGGGA+AGG - Chr8:42341326-42341345 MsG0880044452.01.T01:intron 90.0%
Chromosome Type Strat End Strand Name
Chr8 gene 42340741 42342559 42340741 ID=MsG0880044452.01;Name=MsG0880044452.01
Chr8 mRNA 42340741 42342559 42340741 ID=MsG0880044452.01.T01;Parent=MsG0880044452.01;Name=MsG0880044452.01.T01;_AED=0.67;_eAED=0.68;_QI=0|0|0|0.5|0|0.5|2|0|188
Chr8 exon 42342496 42342559 42342496 ID=MsG0880044452.01.T01:exon:17206;Parent=MsG0880044452.01.T01
Chr8 exon 42340741 42341243 42340741 ID=MsG0880044452.01.T01:exon:17205;Parent=MsG0880044452.01.T01
Chr8 CDS 42342496 42342559 42342496 ID=MsG0880044452.01.T01:cds;Parent=MsG0880044452.01.T01
Chr8 CDS 42340741 42341243 42340741 ID=MsG0880044452.01.T01:cds;Parent=MsG0880044452.01.T01
Gene Sequence

>MsG0880044452.01.T01

ATGGAGACTAAAACCTGTGCGGGGGTAGGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTATAATGTTGATAATGCGGGTGATGATCTGGGTGATCGAAAACCTAGAGTTGTCGATTCCATTAAGGTTGAAGCTCCCATTAAGGTCGAAGCTTCCGTTAAATTTGAGGCTTCAAGCGTCAATTCCGATGTGTGGAAACTTCGTGACAACGAAGTGGACACGGCTCATTTATTTTCAACCCGAGACACATGGAAAGATAAAGATGAATTGCTCGGTTGTGTCCGTCGACAAGCAAATAGGGCTGGATTTACGGTTATCATTAGAAGATCTTGTGAAGGAAGAAATGCTATGTTGGAGTTGGTTTGTGAACGGAGCGGCGAGCACAAAGTACCGAAGACAAAAGTGAAGCATGAAGGAAAAGGATCAAGAAAATGTGGGTGTTTGTTCAAGGTGCGTGGATATGTCGTTAGGGAAGACAATGCTTGGAAATTGGCTATTCTTAATGGCGTTCACAACCATGAGATGGTGCCGTATGTAGCAGACACCTTCTTGCCGGAAGATTAA
Protein sequence

>MsG0880044452.01.T01

METKTCAGVGADVAKWIKAVDYNVDNAGDDLGDRKPRVVDSIKVEAPIKVEASVKFEASSVNSDVWKLRDNEVDTAHLFSTRDTWKDKDELLGCVRRQANRAGFTVIIRRSCEGRNAMLELVCERSGEHKVPKTKVKHEGKGSRKCGCLFKVRGYVVREDNAWKLAILNGVHNHEMVPYVADTFLPED*