Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045009.01.T01 | XP_003628696.1 | 93.056 | 144 | 10 | 0 | 1 | 144 | 1 | 144 | 1.37E-92 | 274 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045009.01.T01 | F4JZT3 | 55.932 | 118 | 50 | 1 | 10 | 127 | 16 | 131 | 1.53E-35 | 122 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045009.01.T01 | G7LG58 | 93.056 | 144 | 10 | 0 | 1 | 144 | 1 | 144 | 6.54e-93 | 274 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0880045009.01.T01 | TR | Others |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045009.01.T01 | MTR_8g063580 | 93.056 | 144 | 10 | 0 | 1 | 144 | 1 | 144 | 1.66e-96 | 274 |
| MsG0880045009.01.T01 | MTR_7g099500 | 67.424 | 132 | 37 | 3 | 1 | 132 | 1 | 126 | 4.74e-53 | 164 |
| MsG0880045009.01.T01 | MTR_7g098910 | 57.600 | 125 | 50 | 1 | 5 | 129 | 15 | 136 | 1.66e-41 | 135 |
| MsG0880045009.01.T01 | MTR_0022s0450 | 54.098 | 61 | 28 | 0 | 18 | 78 | 40 | 100 | 2.01e-14 | 65.1 |
| MsG0880045009.01.T01 | MTR_8g075340 | 31.206 | 141 | 74 | 4 | 12 | 131 | 1029 | 1167 | 2.74e-13 | 66.2 |
| MsG0880045009.01.T01 | MTR_8g106150 | 31.159 | 138 | 67 | 4 | 15 | 132 | 864 | 993 | 3.90e-13 | 65.9 |
| MsG0880045009.01.T01 | MTR_8g106150 | 31.250 | 128 | 63 | 3 | 15 | 122 | 621 | 743 | 4.34e-13 | 65.5 |
| MsG0880045009.01.T01 | MTR_6g008860 | 33.333 | 108 | 70 | 1 | 12 | 119 | 33 | 138 | 8.32e-13 | 62.0 |
| MsG0880045009.01.T01 | MTR_4g071650 | 33.333 | 108 | 70 | 1 | 12 | 119 | 33 | 138 | 8.32e-13 | 62.0 |
| MsG0880045009.01.T01 | MTR_7g098860 | 45.122 | 82 | 42 | 2 | 31 | 111 | 8 | 87 | 1.86e-12 | 60.1 |
| MsG0880045009.01.T01 | MTR_5g097410 | 41.791 | 67 | 39 | 0 | 15 | 81 | 1077 | 1143 | 3.17e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045009.01.T01 | AT5G26594 | 55.932 | 118 | 50 | 1 | 10 | 127 | 16 | 131 | 1.55e-36 | 122 |
| MsG0880045009.01.T01 | AT3G04280 | 51.786 | 112 | 52 | 2 | 12 | 122 | 22 | 132 | 6.27e-32 | 111 |
| MsG0880045009.01.T01 | AT3G04280 | 51.786 | 112 | 52 | 2 | 12 | 122 | 22 | 132 | 6.27e-32 | 111 |
| MsG0880045009.01.T01 | AT3G04280 | 51.786 | 112 | 52 | 2 | 12 | 122 | 22 | 132 | 6.27e-32 | 111 |
| MsG0880045009.01.T01 | AT2G17820 | 27.950 | 161 | 78 | 4 | 15 | 139 | 1047 | 1205 | 2.93e-13 | 66.2 |
| MsG0880045009.01.T01 | AT2G01830 | 30.579 | 121 | 66 | 3 | 15 | 122 | 937 | 1052 | 4.86e-12 | 62.8 |
| MsG0880045009.01.T01 | AT2G01830 | 30.579 | 121 | 66 | 3 | 15 | 122 | 948 | 1063 | 5.21e-12 | 62.8 |
| MsG0880045009.01.T01 | AT2G01830 | 30.579 | 121 | 66 | 3 | 15 | 122 | 925 | 1040 | 5.25e-12 | 62.8 |
| MsG0880045009.01.T01 | AT2G01830 | 30.579 | 121 | 66 | 3 | 15 | 122 | 925 | 1040 | 5.25e-12 | 62.8 |
| MsG0880045009.01.T01 | AT2G01830 | 30.579 | 121 | 66 | 3 | 15 | 122 | 925 | 1040 | 5.25e-12 | 62.8 |
| MsG0880045009.01.T01 | AT2G01830 | 30.579 | 121 | 66 | 3 | 15 | 122 | 925 | 1040 | 5.25e-12 | 62.8 |
| MsG0880045009.01.T01 | AT2G47430 | 33.333 | 123 | 62 | 5 | 15 | 119 | 989 | 1109 | 1.38e-11 | 61.6 |
Find 25 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAAATAGTGATAAAATTTA+TGG | 0.245050 | 8:-52712416 | MsG0880045009.01.T01:CDS |
| TGGTGTATCCTCACACTCTA+TGG | 0.342570 | 8:-52712446 | MsG0880045009.01.T01:CDS |
| TGACCTAATCTTGATGGATA+AGG | 0.347722 | 8:-52712723 | MsG0880045009.01.T01:CDS |
| ACGTCATTTGATGAATTCTT+CGG | 0.351042 | 8:+52712825 | None:intergenic |
| CACAACAAGTGCTGTTAACT+TGG | 0.355344 | 8:+52712865 | None:intergenic |
| CGTCATTTGATGAATTCTTC+GGG | 0.366651 | 8:+52712826 | None:intergenic |
| GTGATAAAATTTATGGAAGC+AGG | 0.456780 | 8:-52712409 | MsG0880045009.01.T01:CDS |
| AATTCCATTCTTCATGCCGT+TGG | 0.460947 | 8:-52712343 | MsG0880045009.01.T01:CDS |
| TATCCTTATCCATCAAGATT+AGG | 0.462938 | 8:+52712720 | None:intergenic |
| TCTTGAGAAACCATTGGATA+AGG | 0.467024 | 8:-52712374 | MsG0880045009.01.T01:CDS |
| AGGATATCTAGCACTATTGT+TGG | 0.469888 | 8:-52712466 | MsG0880045009.01.T01:CDS |
| TCATTTCATCTTGTGAGGAA+TGG | 0.470437 | 8:+52712297 | None:intergenic |
| TATAGGCAACAAAGCAACTA+AGG | 0.474253 | 8:-52712495 | MsG0880045009.01.T01:intron |
| CAAATGACGTTGAGTAACTT+AGG | 0.479404 | 8:-52712812 | MsG0880045009.01.T01:CDS |
| ACCTCAGCTCCATTCATCTC+AGG | 0.491878 | 8:+52712695 | None:intergenic |
| AAGGATATGCCTGAGATGAA+TGG | 0.499108 | 8:-52712704 | MsG0880045009.01.T01:CDS |
| CGAATATCTTGAGAAACCAT+TGG | 0.533361 | 8:-52712380 | MsG0880045009.01.T01:CDS |
| TTTGTTCATTTCATCTTGTG+AGG | 0.533712 | 8:+52712292 | None:intergenic |
| CTCCTCTTCCATAGAGTGTG+AGG | 0.555361 | 8:+52712438 | None:intergenic |
| ATCCTCACACTCTATGGAAG+AGG | 0.570487 | 8:-52712440 | MsG0880045009.01.T01:CDS |
| CAAAGCAACTAAGGTTGATG+AGG | 0.578109 | 8:-52712486 | MsG0880045009.01.T01:CDS |
| CAAGTTAACAGCACTTGTTG+TGG | 0.584291 | 8:-52712864 | MsG0880045009.01.T01:CDS |
| CTCACACTCTATGGAAGAGG+AGG | 0.604784 | 8:-52712437 | MsG0880045009.01.T01:CDS |
| GCAGTGGAAATTCATCAGCA+TGG | 0.649906 | 8:-52712755 | MsG0880045009.01.T01:CDS |
| GCCTGAGATGAATGGAGCTG+AGG | 0.680215 | 8:-52712696 | MsG0880045009.01.T01:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTATGTGTTTCTTATAT+AGG | - | Chr8:52712678-52712697 | MsG0880045009.01.T01:intron | 15.0% |
| !!! | TTTTTCTTTTATTTTTCCAA+CGG | + | Chr8:52712866-52712885 | None:intergenic | 15.0% |
| !! | GGAAATAGTGATAAAATTTA+TGG | - | Chr8:52712774-52712793 | MsG0880045009.01.T01:CDS | 20.0% |
| !!! | ATTTTTTGACCTAATCTTGA+TGG | - | Chr8:52712461-52712480 | MsG0880045009.01.T01:CDS | 25.0% |
| AACAACACTTGTGTGAAAAA+CGG | - | Chr8:52712405-52712424 | MsG0880045009.01.T01:CDS | 30.0% | |
| ACGTCATTTGATGAATTCTT+CGG | + | Chr8:52712368-52712387 | None:intergenic | 30.0% | |
| GAGTTCATTCTTGCTTATTT+AGG | - | Chr8:52712576-52712595 | MsG0880045009.01.T01:intron | 30.0% | |
| GTGATAAAATTTATGGAAGC+AGG | - | Chr8:52712781-52712800 | MsG0880045009.01.T01:CDS | 30.0% | |
| TATCCTTATCCATCAAGATT+AGG | + | Chr8:52712473-52712492 | None:intergenic | 30.0% | |
| !!! | TTGGATGTAAAGTTTTGAGA+TGG | + | Chr8:52712309-52712328 | None:intergenic | 30.0% |
| AGGATATCTAGCACTATTGT+TGG | - | Chr8:52712724-52712743 | MsG0880045009.01.T01:CDS | 35.0% | |
| CAAATGACGTTGAGTAACTT+AGG | - | Chr8:52712378-52712397 | MsG0880045009.01.T01:CDS | 35.0% | |
| CGAATATCTTGAGAAACCAT+TGG | - | Chr8:52712810-52712829 | MsG0880045009.01.T01:CDS | 35.0% | |
| CGTCATTTGATGAATTCTTC+GGG | + | Chr8:52712367-52712386 | None:intergenic | 35.0% | |
| TATAGGCAACAAAGCAACTA+AGG | - | Chr8:52712695-52712714 | MsG0880045009.01.T01:intron | 35.0% | |
| TCATTTCATCTTGTGAGGAA+TGG | + | Chr8:52712896-52712915 | None:intergenic | 35.0% | |
| TCTTGAGAAACCATTGGATA+AGG | - | Chr8:52712816-52712835 | MsG0880045009.01.T01:CDS | 35.0% | |
| ! | TGACCTAATCTTGATGGATA+AGG | - | Chr8:52712467-52712486 | MsG0880045009.01.T01:CDS | 35.0% |
| ! | TTGAGTTTTGCCTTATCCAA+TGG | + | Chr8:52712829-52712848 | None:intergenic | 35.0% |
| AAGGATATGCCTGAGATGAA+TGG | - | Chr8:52712486-52712505 | MsG0880045009.01.T01:CDS | 40.0% | |
| AATTCCATTCTTCATGCCGT+TGG | - | Chr8:52712847-52712866 | MsG0880045009.01.T01:CDS | 40.0% | |
| CATTCTTGCTTATTTAGGCG+TGG | - | Chr8:52712581-52712600 | MsG0880045009.01.T01:intron | 40.0% | |
| GAAAAACGGAAAAGAAGCAG+TGG | - | Chr8:52712419-52712438 | MsG0880045009.01.T01:CDS | 40.0% | |
| ! | CAAGTTAACAGCACTTGTTG+TGG | - | Chr8:52712326-52712345 | MsG0880045009.01.T01:CDS | 40.0% |
| ! | CACAACAAGTGCTGTTAACT+TGG | + | Chr8:52712328-52712347 | None:intergenic | 40.0% |
| ! | TTTTCCAACGGCATGAAGAA+TGG | + | Chr8:52712854-52712873 | None:intergenic | 40.0% |
| !! | CAAAGCAACTAAGGTTGATG+AGG | - | Chr8:52712704-52712723 | MsG0880045009.01.T01:CDS | 40.0% |
| ATCCTCACACTCTATGGAAG+AGG | - | Chr8:52712750-52712769 | MsG0880045009.01.T01:CDS | 45.0% | |
| GCAGTGGAAATTCATCAGCA+TGG | - | Chr8:52712435-52712454 | MsG0880045009.01.T01:CDS | 45.0% | |
| TGGTGTATCCTCACACTCTA+TGG | - | Chr8:52712744-52712763 | MsG0880045009.01.T01:CDS | 45.0% | |
| !! | ATATATAAATGTAAAATTTA+GGG | + | Chr8:52712540-52712559 | None:intergenic | 5.0% |
| !! | TATATATAAATGTAAAATTT+AGG | + | Chr8:52712541-52712560 | None:intergenic | 5.0% |
| ACCTCAGCTCCATTCATCTC+AGG | + | Chr8:52712498-52712517 | None:intergenic | 50.0% | |
| CTCACACTCTATGGAAGAGG+AGG | - | Chr8:52712753-52712772 | MsG0880045009.01.T01:CDS | 50.0% | |
| CTCCTCTTCCATAGAGTGTG+AGG | + | Chr8:52712755-52712774 | None:intergenic | 50.0% | |
| GCCTGAGATGAATGGAGCTG+AGG | - | Chr8:52712494-52712513 | MsG0880045009.01.T01:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 52712297 | 52712915 | 52712297 | ID=MsG0880045009.01;Name=MsG0880045009.01 |
| Chr8 | mRNA | 52712297 | 52712915 | 52712297 | ID=MsG0880045009.01.T01;Parent=MsG0880045009.01;Name=MsG0880045009.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0|0|1|1|1|2|0|144 |
| Chr8 | exon | 52712697 | 52712915 | 52712697 | ID=MsG0880045009.01.T01:exon:22626;Parent=MsG0880045009.01.T01 |
| Chr8 | exon | 52712297 | 52712512 | 52712297 | ID=MsG0880045009.01.T01:exon:22625;Parent=MsG0880045009.01.T01 |
| Chr8 | CDS | 52712697 | 52712915 | 52712697 | ID=MsG0880045009.01.T01:cds;Parent=MsG0880045009.01.T01 |
| Chr8 | CDS | 52712297 | 52712512 | 52712297 | ID=MsG0880045009.01.T01:cds;Parent=MsG0880045009.01.T01 |
| Gene Sequence |
| Protein sequence |
