AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045134.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045134.01.T01 MTR_8g095330 64.583 192 54 4 2 193 24 201 6.18e-75 229
MsG0880045134.01.T01 MTR_3g097320 64.062 192 55 3 2 193 23 200 1.24e-74 229
MsG0880045134.01.T01 MTR_2g006330 63.830 188 54 4 6 193 27 200 6.19e-74 227
MsG0880045134.01.T01 MTR_4g099240 63.068 176 55 3 18 193 36 201 1.49e-70 218
MsG0880045134.01.T01 MTR_8g089975 63.068 176 55 3 18 193 35 200 3.20e-70 217
MsG0880045134.01.T01 MTR_1g034030 62.500 176 56 3 18 193 55 220 1.52e-69 216
MsG0880045134.01.T01 MTR_1g034030 62.500 176 56 3 18 193 55 220 2.65e-69 214
MsG0880045134.01.T01 MTR_4g086855 61.932 176 57 3 18 193 54 219 4.72e-69 215
MsG0880045134.01.T01 MTR_5g064540 60.227 176 60 3 18 193 35 200 4.88e-68 209
MsG0880045134.01.T01 MTR_5g013550 60.795 176 59 3 18 193 37 202 1.06e-67 211
MsG0880045134.01.T01 MTR_8g079560 60.556 180 61 3 18 197 54 223 2.67e-67 210
MsG0880045134.01.T01 MTR_5g064540 60.227 176 60 3 18 193 35 200 9.02e-67 208
MsG0880045134.01.T01 MTR_3g086940 60.795 176 59 3 18 193 36 201 1.60e-65 205
MsG0880045134.01.T01 MTR_5g018050 59.091 176 62 3 18 193 49 214 1.69e-65 202
MsG0880045134.01.T01 MTR_5g018050 59.091 176 62 3 18 193 49 214 2.04e-64 202
MsG0880045134.01.T01 MTR_5g018050 60.234 171 58 3 23 193 21 181 3.26e-64 201
MsG0880045134.01.T01 MTR_8g088740 53.125 160 62 3 22 181 39 185 3.01e-54 171
MsG0880045134.01.T01 MTR_4g099220 52.841 176 73 3 18 193 42 207 3.80e-54 176
MsG0880045134.01.T01 MTR_2g006330 62.406 133 40 3 61 193 2 124 1.07e-47 157
MsG0880045134.01.T01 MTR_8g089975 60.902 133 42 3 61 193 2 124 1.97e-46 154
MsG0880045134.01.T01 MTR_4g086855 57.895 133 46 3 61 193 2 124 1.87e-44 149
MsG0880045134.01.T01 MTR_5g075100 38.150 173 83 6 27 192 67 222 2.17e-24 99.8
MsG0880045134.01.T01 MTR_2g049790 33.684 190 101 7 21 202 61 233 7.95e-24 98.2
MsG0880045134.01.T01 MTR_5g088350 32.057 209 117 7 2 202 32 223 2.30e-21 90.1
MsG0880045134.01.T01 MTR_3g466400 35.602 191 85 9 26 202 57 223 2.96e-21 90.9
MsG0880045134.01.T01 MTR_5g088350 32.057 209 117 7 2 202 32 223 4.54e-21 90.5
MsG0880045134.01.T01 MTR_5g088350 32.828 198 109 6 2 192 32 212 6.44e-21 89.7
MsG0880045134.01.T01 MTR_1g013700 34.595 185 95 8 26 202 56 222 2.05e-20 88.2
MsG0880045134.01.T01 MTR_3g466480 36.702 188 93 8 23 202 61 230 2.46e-20 88.2
MsG0880045134.01.T01 MTR_4g029020 33.333 210 108 11 1 202 36 221 4.05e-20 87.8
MsG0880045134.01.T01 MTR_5g075060 34.973 183 93 8 28 202 57 221 6.21e-19 84.3
MsG0880045134.01.T01 MTR_2g016340 33.673 196 103 8 2 192 37 210 7.39e-19 84.0
MsG0880045134.01.T01 MTR_4g006970 35.119 168 88 7 26 187 63 215 7.51e-19 84.0
MsG0880045134.01.T01 MTR_7g072575 32.461 191 103 8 20 202 49 221 2.19e-18 82.8
MsG0880045134.01.T01 MTR_8g086380 35.556 180 77 9 27 192 70 224 5.77e-18 81.6
MsG0880045134.01.T01 MTR_8g024600 32.609 184 109 6 21 202 55 225 7.92e-18 81.3
MsG0880045134.01.T01 MTR_4g114670 32.370 173 95 6 21 187 49 205 8.35e-18 80.9
MsG0880045134.01.T01 MTR_4g114670 32.370 173 95 6 21 187 49 205 9.33e-18 80.9
MsG0880045134.01.T01 MTR_2g045470 31.609 174 95 7 26 192 63 219 1.06e-17 80.9
MsG0880045134.01.T01 MTR_8g024590 32.065 184 110 6 21 202 55 225 1.59e-17 80.1
MsG0880045134.01.T01 MTR_1g076370 34.831 178 91 8 23 192 61 221 1.72e-17 80.1
MsG0880045134.01.T01 MTR_5g067150 30.412 194 117 6 2 192 28 206 5.18e-17 78.6
MsG0880045134.01.T01 MTR_5g067150 30.412 194 117 6 2 192 28 206 6.04e-17 78.6
MsG0880045134.01.T01 MTR_5g067150 30.412 194 117 6 2 192 28 206 7.03e-17 78.6
MsG0880045134.01.T01 MTR_6g012990 34.337 166 96 4 23 187 59 212 8.31e-17 77.8
MsG0880045134.01.T01 MTR_4g123940 31.638 177 97 7 23 192 66 225 1.27e-16 77.0
MsG0880045134.01.T01 MTR_6g012990 34.337 166 96 4 23 187 59 212 1.32e-16 77.8
MsG0880045134.01.T01 MTR_4g123940 31.638 177 97 7 23 192 66 225 1.42e-16 77.4
MsG0880045134.01.T01 MTR_6g012980 32.530 166 99 4 23 187 113 266 1.92e-16 77.0
MsG0880045134.01.T01 MTR_4g131060 31.351 185 101 8 26 202 61 227 2.22e-16 77.0
MsG0880045134.01.T01 MTR_4g128820 32.086 187 105 8 21 202 48 217 2.66e-16 76.6
MsG0880045134.01.T01 MTR_6g048250 33.735 166 97 4 23 187 59 212 2.95e-16 76.3
MsG0880045134.01.T01 MTR_6g048250 33.735 166 97 4 23 187 59 212 4.60e-16 75.9
MsG0880045134.01.T01 MTR_6g048250 33.735 166 97 4 23 187 59 212 5.67e-16 75.9
MsG0880045134.01.T01 MTR_4g066660 35.294 170 96 7 21 187 109 267 8.28e-16 75.5
MsG0880045134.01.T01 MTR_1g040875 33.333 195 89 10 23 201 64 233 1.03e-15 75.1
MsG0880045134.01.T01 MTR_8g024100 30.822 146 93 2 24 168 55 193 2.84e-15 73.9
MsG0880045134.01.T01 MTR_4g019410 28.571 154 102 2 16 168 51 197 2.87e-15 73.9
MsG0880045134.01.T01 MTR_3g099920 32.530 166 99 5 23 187 59 212 3.52e-15 73.6
MsG0880045134.01.T01 MTR_2g105010 33.333 183 100 8 26 202 57 223 3.82e-15 73.6
MsG0880045134.01.T01 MTR_4g019410 28.571 154 102 2 16 168 51 197 8.36e-15 72.8
MsG0880045134.01.T01 MTR_1g103760 29.016 193 118 5 2 191 37 213 1.67e-14 71.6
MsG0880045134.01.T01 MTR_3g072320 29.670 182 96 6 31 202 60 219 4.20e-14 70.5
MsG0880045134.01.T01 MTR_1g050690 31.953 169 101 6 26 190 457 615 6.53e-14 70.1
MsG0880045134.01.T01 MTR_8g035890 31.847 157 92 6 21 177 54 195 3.16e-13 67.0
MsG0880045134.01.T01 MTR_4g107490 30.851 188 113 8 21 203 110 285 9.80e-13 66.6
MsG0880045134.01.T01 MTR_4g114670 28.324 173 89 6 21 187 49 192 1.29e-12 66.2
MsG0880045134.01.T01 MTR_8g107000 35.526 152 86 6 26 174 769 911 1.47e-12 66.2
MsG0880045134.01.T01 MTR_7g091890 34.247 146 86 4 21 163 95 233 2.03e-12 65.5
MsG0880045134.01.T01 MTR_8g040300 28.662 157 97 5 21 177 48 189 3.75e-12 63.9
MsG0880045134.01.T01 MTR_7g091890 34.247 146 86 4 21 163 95 233 6.82e-12 63.9
MsG0880045134.01.T01 MTR_7g072605 32.370 173 93 6 27 192 61 216 9.07e-12 63.5
MsG0880045134.01.T01 MTR_1g048630 33.793 145 85 6 27 170 50 184 9.11e-12 63.9
MsG0880045134.01.T01 MTR_5g092810 32.911 158 93 6 31 187 142 287 1.00e-11 63.5
MsG0880045134.01.T01 MTR_4g107280 35.246 122 70 2 40 161 153 265 1.90e-11 62.8
MsG0880045134.01.T01 MTR_3g098090 33.333 138 77 5 31 164 82 208 2.76e-11 62.4
MsG0880045134.01.T01 MTR_4g082325 32.609 138 83 2 38 170 196 328 3.10e-11 62.0
MsG0880045134.01.T01 MTR_6g079540 33.929 168 97 8 26 190 452 608 3.66e-11 62.0
MsG0880045134.01.T01 MTR_1g041150 33.333 141 85 3 26 164 161 294 3.77e-11 62.0
MsG0880045134.01.T01 MTR_6g079540 33.929 168 97 8 26 190 700 856 4.11e-11 62.0
MsG0880045134.01.T01 MTR_3g051770 35.556 135 78 3 31 164 134 260 4.13e-11 61.6
MsG0880045134.01.T01 MTR_3g095620 31.818 132 82 2 37 167 78 202 7.82e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045134.01.T01 AT1G10940 64.362 188 53 4 6 193 27 200 5.68e-74 228
MsG0880045134.01.T01 AT1G10940 62.245 196 52 4 6 193 27 208 1.21e-73 227
MsG0880045134.01.T01 AT5G63650 63.830 188 54 4 6 193 27 200 1.90e-71 221
MsG0880045134.01.T01 AT1G60940 62.766 188 56 4 6 193 27 200 5.06e-71 220
MsG0880045134.01.T01 AT1G60940 62.766 188 56 4 6 193 27 200 5.06e-71 220
MsG0880045134.01.T01 AT5G08590 60.938 192 61 4 6 197 27 204 8.39e-70 216
MsG0880045134.01.T01 AT4G33950 63.068 176 55 3 18 193 52 217 9.77e-70 216
MsG0880045134.01.T01 AT4G33950 63.068 176 55 3 18 193 52 217 1.06e-69 216
MsG0880045134.01.T01 AT3G50500 63.068 176 55 3 18 193 54 219 1.28e-69 216
MsG0880045134.01.T01 AT5G66880 62.500 176 56 3 18 193 53 218 2.47e-69 216
MsG0880045134.01.T01 AT5G66880 62.500 176 56 3 18 193 53 218 2.47e-69 216
MsG0880045134.01.T01 AT3G50500 60.656 183 55 4 18 193 54 226 6.44e-67 209
MsG0880045134.01.T01 AT4G40010 61.364 176 58 3 18 193 35 200 1.37e-65 206
MsG0880045134.01.T01 AT2G23030 57.812 192 67 4 2 193 23 200 3.68e-64 202
MsG0880045134.01.T01 AT1G78290 59.659 176 61 3 18 193 35 200 8.68e-63 198
MsG0880045134.01.T01 AT1G78290 59.659 176 61 3 18 193 35 200 8.68e-63 198
MsG0880045134.01.T01 AT5G08590 59.124 137 46 3 61 197 2 128 2.58e-44 149
MsG0880045134.01.T01 AT4G11373 74.242 66 17 0 18 83 4 69 1.02e-26 99.0
MsG0880045134.01.T01 AT2G26980 33.684 190 101 7 21 202 52 224 2.19e-23 94.4
MsG0880045134.01.T01 AT2G26980 33.014 209 115 8 2 202 33 224 1.41e-22 94.0
MsG0880045134.01.T01 AT2G26980 33.014 209 115 8 2 202 33 224 1.91e-22 94.4
MsG0880045134.01.T01 AT5G01810 36.842 190 94 9 21 202 50 221 2.11e-22 93.6
MsG0880045134.01.T01 AT2G26980 33.014 209 115 8 2 202 43 234 2.38e-22 94.0
MsG0880045134.01.T01 AT2G26980 34.211 190 100 8 21 202 52 224 2.48e-22 94.0
MsG0880045134.01.T01 AT5G01810 37.766 188 95 9 21 202 50 221 2.56e-22 93.6
MsG0880045134.01.T01 AT5G01810 37.766 188 95 9 21 202 50 221 2.56e-22 93.6
MsG0880045134.01.T01 AT1G30270 33.880 183 96 7 28 202 76 241 1.84e-21 91.7
MsG0880045134.01.T01 AT1G30270 33.880 183 96 7 28 202 76 241 1.85e-21 91.7
MsG0880045134.01.T01 AT3G23000 33.168 202 112 7 8 202 50 235 5.00e-21 90.1
MsG0880045134.01.T01 AT5G21326 31.905 210 118 8 1 202 31 223 7.71e-21 89.7
MsG0880045134.01.T01 AT1G01140 32.461 191 104 8 20 202 56 229 1.24e-20 89.4
MsG0880045134.01.T01 AT1G01140 32.461 191 104 8 20 202 56 229 1.32e-20 89.0
MsG0880045134.01.T01 AT1G01140 32.461 191 104 8 20 202 56 229 1.39e-20 89.0
MsG0880045134.01.T01 AT2G25090 32.487 197 101 7 21 202 54 233 2.01e-20 88.6
MsG0880045134.01.T01 AT4G14580 32.828 198 103 8 18 202 52 232 2.29e-20 88.2
MsG0880045134.01.T01 AT5G35410 36.471 170 90 6 26 192 54 208 2.51e-20 87.8
MsG0880045134.01.T01 AT5G35410 36.471 170 90 6 26 192 54 208 3.42e-20 87.8
MsG0880045134.01.T01 AT1G48260 32.961 179 94 8 21 189 49 211 1.03e-19 86.7
MsG0880045134.01.T01 AT5G10930 35.754 179 88 8 21 189 51 212 1.30e-19 86.3
MsG0880045134.01.T01 AT5G25110 34.286 175 90 7 21 187 81 238 1.73e-19 85.9
MsG0880045134.01.T01 AT3G17510 32.948 173 90 8 27 189 64 220 2.09e-19 85.5
MsG0880045134.01.T01 AT5G58380 32.973 185 98 9 26 202 55 221 3.25e-19 85.1
MsG0880045134.01.T01 AT2G30360 34.682 173 89 7 27 192 66 221 3.60e-19 85.1
MsG0880045134.01.T01 AT5G45810 34.239 184 95 8 27 202 72 237 2.09e-18 82.8
MsG0880045134.01.T01 AT5G39440 36.527 167 90 5 23 187 59 211 5.37e-18 81.6
MsG0880045134.01.T01 AT5G21222 32.000 175 103 5 21 192 51 212 6.36e-18 81.6
MsG0880045134.01.T01 AT5G21222 32.000 175 103 5 21 192 51 212 6.36e-18 81.6
MsG0880045134.01.T01 AT5G21222 32.000 175 103 5 21 192 51 212 6.36e-18 81.6
MsG0880045134.01.T01 AT5G21222 32.000 175 103 5 21 192 51 212 6.36e-18 81.6
MsG0880045134.01.T01 AT4G24400 34.706 170 93 6 26 192 52 206 6.63e-18 81.3
MsG0880045134.01.T01 AT3G01090 35.329 167 93 5 23 187 82 235 7.16e-18 81.6
MsG0880045134.01.T01 AT3G01090 35.329 167 93 5 23 187 59 212 7.19e-18 81.6
MsG0880045134.01.T01 AT3G01090 35.329 167 93 5 23 187 59 212 7.19e-18 81.6
MsG0880045134.01.T01 AT4G24400 34.706 170 93 6 26 192 52 206 9.02e-18 80.9
MsG0880045134.01.T01 AT4G18700 34.783 184 94 9 27 202 70 235 9.58e-18 80.9
MsG0880045134.01.T01 AT3G53930 33.333 147 88 3 24 168 60 198 4.20e-17 79.3
MsG0880045134.01.T01 AT3G53930 33.333 147 88 3 24 168 60 198 4.20e-17 79.3
MsG0880045134.01.T01 AT3G29160 34.731 167 94 5 23 187 60 213 5.41e-17 78.2
MsG0880045134.01.T01 AT2G38490 34.054 185 94 8 27 202 72 237 7.82e-17 78.2
MsG0880045134.01.T01 AT3G29160 34.731 167 94 5 23 187 60 213 1.23e-16 77.8
MsG0880045134.01.T01 AT3G29160 34.731 167 94 5 23 187 60 213 1.23e-16 77.8
MsG0880045134.01.T01 AT4G30960 32.973 185 98 8 26 202 67 233 1.67e-16 77.4
MsG0880045134.01.T01 AT2G34180 34.444 180 100 8 27 202 101 266 2.35e-16 77.0
MsG0880045134.01.T01 AT3G61960 31.507 146 92 2 24 168 50 188 7.91e-16 75.5
MsG0880045134.01.T01 AT3G61960 31.507 146 92 2 24 168 50 188 1.03e-15 75.5
MsG0880045134.01.T01 AT5G01820 35.754 179 90 7 21 192 60 220 1.17e-15 75.1
MsG0880045134.01.T01 AT1G29230 33.152 184 97 8 27 202 118 283 1.28e-15 75.1
MsG0880045134.01.T01 AT2G37840 30.822 146 93 2 24 168 52 190 7.95e-15 72.8
MsG0880045134.01.T01 AT2G31500 34.503 171 96 7 21 187 103 261 1.40e-14 72.0
MsG0880045134.01.T01 AT5G07070 31.579 190 104 8 21 202 50 221 1.86e-14 71.6
MsG0880045134.01.T01 AT5G03730 39.259 135 71 6 31 161 596 723 2.01e-13 68.6
MsG0880045134.01.T01 AT5G03730 39.259 135 71 6 31 161 596 723 2.01e-13 68.6
MsG0880045134.01.T01 AT5G57630 28.415 183 117 5 9 191 38 206 2.86e-13 68.2
MsG0880045134.01.T01 AT5G57630 28.415 183 117 5 9 191 38 206 2.91e-13 67.8
MsG0880045134.01.T01 AT2G45490 30.573 157 94 4 21 177 60 201 5.33e-13 66.2
MsG0880045134.01.T01 AT2G45490 30.573 157 94 4 21 177 60 201 9.37e-13 65.9
MsG0880045134.01.T01 AT2G41860 32.941 170 100 7 21 187 91 249 1.32e-12 66.2
MsG0880045134.01.T01 AT2G41860 32.941 170 100 7 21 187 91 249 1.37e-12 66.2
MsG0880045134.01.T01 AT1G35670 35.417 144 76 5 26 163 69 201 2.01e-12 65.5
MsG0880045134.01.T01 AT1G74740 30.319 188 114 8 21 203 96 271 2.35e-12 65.5
MsG0880045134.01.T01 AT3G57530 34.694 147 84 5 21 163 100 238 2.53e-12 65.1
MsG0880045134.01.T01 AT4G09570 36.111 144 75 5 26 163 68 200 2.90e-12 65.1
MsG0880045134.01.T01 AT1G18160 36.552 145 81 6 26 167 755 891 6.43e-12 64.3
MsG0880045134.01.T01 AT1G18160 36.552 145 81 6 26 167 755 891 6.47e-12 64.3
MsG0880045134.01.T01 AT3G57530 34.694 147 84 5 21 163 100 238 6.64e-12 64.3
MsG0880045134.01.T01 AT3G06620 30.519 154 92 5 19 167 527 670 6.75e-12 64.3
MsG0880045134.01.T01 AT3G06630 30.519 154 92 5 19 167 467 610 1.06e-11 63.5
MsG0880045134.01.T01 AT3G06630 30.519 154 92 5 19 167 467 610 1.06e-11 63.5
MsG0880045134.01.T01 AT3G06630 30.519 154 92 5 19 167 467 610 1.06e-11 63.5
MsG0880045134.01.T01 AT3G06630 30.519 154 92 5 19 167 467 610 1.07e-11 63.5
MsG0880045134.01.T01 AT3G06630 30.519 154 92 5 19 167 467 610 1.08e-11 63.5
MsG0880045134.01.T01 AT1G73660 35.862 145 82 6 26 167 788 924 1.40e-11 63.5
MsG0880045134.01.T01 AT5G49470 31.169 154 91 5 19 167 397 540 1.80e-11 62.8
MsG0880045134.01.T01 AT5G49470 31.169 154 91 5 19 167 524 667 1.84e-11 62.8
MsG0880045134.01.T01 AT5G49470 31.169 154 91 5 19 167 524 667 1.84e-11 62.8
MsG0880045134.01.T01 AT5G49470 31.169 154 91 5 19 167 498 641 1.86e-11 62.8
MsG0880045134.01.T01 AT5G49470 31.169 154 91 5 19 167 524 667 1.87e-11 62.8
MsG0880045134.01.T01 AT1G76040 34.375 128 70 3 40 163 170 287 2.43e-11 62.4
MsG0880045134.01.T01 AT2G17290 36.232 138 79 3 26 161 128 258 2.84e-11 62.4
MsG0880045134.01.T01 AT2G17290 36.232 138 79 3 26 161 128 258 2.84e-11 62.4
MsG0880045134.01.T01 AT3G06630 29.670 182 100 8 19 193 467 627 3.37e-11 62.0
MsG0880045134.01.T01 AT1G76040 34.375 128 70 3 40 163 170 287 3.43e-11 62.0
MsG0880045134.01.T01 AT1G14000 31.250 160 92 4 16 170 201 347 7.49e-11 60.8

Find 46 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTGGAACTCCAGCTTATA+TGG 0.248985 8:-54662894 MsG0880045134.01.T01:CDS
GTTACTATTGCTAAATGATT+AGG 0.250308 8:+54663434 None:intergenic
TTACTATTGCTAAATGATTA+GGG 0.261994 8:+54663435 None:intergenic
TCCTTGAAGCGAATTATATT+CGG 0.307244 8:+54663573 None:intergenic
GGAGTTCCAACAATTGATTT+AGG 0.314283 8:+54662905 None:intergenic
GCTGAACCGACCAGCATTAC+AGG 0.337585 8:+54663376 None:intergenic
TGGCACTAGAGGTTCTTTCT+AGG 0.359225 8:-54662874 MsG0880045134.01.T01:CDS
AGGCTTGATGGTGCAATTCA+TGG 0.410361 8:+54664041 None:intergenic
AAGCTTCACCAGAGGCTTGA+TGG 0.417893 8:+54664029 None:intergenic
TCTCGACCTAAATCAATTGT+TGG 0.422033 8:-54662911 MsG0880045134.01.T01:CDS
CCCGAATATAATTCGCTTCA+AGG 0.424576 8:-54663574 MsG0880045134.01.T01:CDS
CAATTGATTTAGGTCGAGAA+TGG 0.463444 8:+54662915 None:intergenic
TGCTTGAAAATCTGTGACTT+CGG 0.478091 8:-54663050 MsG0880045134.01.T01:CDS
CCTTGAAGCGAATTATATTC+GGG 0.486169 8:+54663574 None:intergenic
ATCAAGCCTCTGGTGAAGCT+TGG 0.488088 8:-54664027 MsG0880045134.01.T01:CDS
ACCTCTAGTGCCATATAAGC+TGG 0.492728 8:+54662884 None:intergenic
ATACCGAGTATCGGTATTCA+AGG 0.515193 8:+54663989 None:intergenic
GGCAAACACACAAAGATGTA+AGG 0.518221 8:+54662797 None:intergenic
GAATTGCACCATCAAGCCTC+TGG 0.521090 8:-54664037 MsG0880045134.01.T01:CDS
CACTAGAGGTTCTTTCTAGG+AGG 0.543649 8:-54662871 MsG0880045134.01.T01:CDS
CTTTCTAGGAGGGAGTATGA+TGG 0.545765 8:-54662860 MsG0880045134.01.T01:CDS
GAGATAACAAACATGTATTG+AGG 0.555599 8:+54662776 None:intergenic
TTATCAACCACAGATCTCTT+CGG 0.555612 8:-54663599 MsG0880045134.01.T01:CDS
CTGTGACTTCGGTTATTCCA+AGG 0.566084 8:-54663039 MsG0880045134.01.T01:intron
ATAGTAACACAATATGCAGC+TGG 0.566363 8:-54663419 MsG0880045134.01.T01:CDS
TTTCCAGATTGATGAGAATG+TGG 0.567042 8:-54663631 MsG0880045134.01.T01:intron
TTTGAGAGGACCTGTAATGC+TGG 0.569556 8:-54663386 MsG0880045134.01.T01:CDS
ACTAGAGGTTCTTTCTAGGA+GGG 0.582204 8:-54662870 MsG0880045134.01.T01:CDS
GGTGAAGCTTGGCTTTCTCG+AGG 0.584023 8:-54664016 MsG0880045134.01.T01:CDS
ATGCCACAGAGATTTGAAGC+TGG 0.586548 8:-54663105 MsG0880045134.01.T01:CDS
AGAGGACCTGTAATGCTGGT+CGG 0.592162 8:-54663382 MsG0880045134.01.T01:CDS
TAGGAGGGAGTATGATGGCA+TGG 0.597405 8:-54662855 MsG0880045134.01.T01:CDS
TCCAGCTTATATGGCACTAG+AGG 0.598379 8:-54662885 MsG0880045134.01.T01:CDS
AACACAATATGCAGCTGGAG+GGG 0.600335 8:-54663414 MsG0880045134.01.T01:CDS
TAACACAATATGCAGCTGGA+GGG 0.615840 8:-54663415 MsG0880045134.01.T01:CDS
TACTATTGCTAAATGATTAG+GGG 0.616535 8:+54663436 None:intergenic
GGAGCATGAATACCGAGTAT+CGG 0.619028 8:+54663980 None:intergenic
ATGAGGTTAATAACTTGACA+TGG 0.624734 8:-54663349 MsG0880045134.01.T01:intron
TTGCCTTGAATACCGATACT+CGG 0.626208 8:-54663992 MsG0880045134.01.T01:intron
TGGTCGGTTCAGCGAAGATG+AGG 0.629226 8:-54663366 MsG0880045134.01.T01:CDS
CTGGAGGGGAACTCTTTGAG+AGG 0.634190 8:-54663400 MsG0880045134.01.T01:CDS
TTTCCAGCTTCAAATCTCTG+TGG 0.646446 8:+54663102 None:intergenic
GTAACACAATATGCAGCTGG+AGG 0.654060 8:-54663416 MsG0880045134.01.T01:CDS
TCGGGTGCCGAAGAGATCTG+TGG 0.687028 8:+54663592 None:intergenic
AGAAAGCCAAGCTTCACCAG+AGG 0.702351 8:+54664021 None:intergenic
GAATATAATTCGCTTCAAGG+AGG 0.726990 8:-54663571 MsG0880045134.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAATAACTTTTCAAAATC+AGG + Chr8:54663061-54663080 None:intergenic 15.0%
!!! ATAATAACTTTTCAAAATCA+GGG + Chr8:54663060-54663079 None:intergenic 15.0%
!!! GTTATTATTTGTTGAAAAAA+AGG - Chr8:54663072-54663091 MsG0880045134.01.T01:CDS 15.0%
!! AAATTAAAGAAATTGAACAG+GGG - Chr8:54662988-54663007 MsG0880045134.01.T01:intron 20.0%
!! ACCAATGTTTATTAAAATAG+GGG + Chr8:54662906-54662925 None:intergenic 20.0%
!! CAAATTAAAGAAATTGAACA+GGG - Chr8:54662987-54663006 MsG0880045134.01.T01:intron 20.0%
!! CACCAATGTTTATTAAAATA+GGG + Chr8:54662907-54662926 None:intergenic 20.0%
!! TTACTATTGCTAAATGATTA+GGG + Chr8:54663358-54663377 None:intergenic 20.0%
!!! CATTTTTTGGAAAATTATAC+AGG - Chr8:54663600-54663619 MsG0880045134.01.T01:CDS 20.0%
!!! TAATAACTTTTCAAAATCAG+GGG + Chr8:54663059-54663078 None:intergenic 20.0%
!!! TTGTATTGAGTTTTAAAGTT+TGG - Chr8:54663786-54663805 MsG0880045134.01.T01:intron 20.0%
! CCACCAATGTTTATTAAAAT+AGG + Chr8:54662908-54662927 None:intergenic 25.0%
! GCAAATTAAAGAAATTGAAC+AGG - Chr8:54662986-54663005 MsG0880045134.01.T01:intron 25.0%
! GTTACTATTGCTAAATGATT+AGG + Chr8:54663359-54663378 None:intergenic 25.0%
! TACTATTGCTAAATGATTAG+GGG + Chr8:54663357-54663376 None:intergenic 25.0%
!! TTGTTAACTGTTACTTCAAT+GGG - Chr8:54663306-54663325 MsG0880045134.01.T01:intron 25.0%
!!! AAAGCTATTATTTTGACATC+TGG - Chr8:54663099-54663118 MsG0880045134.01.T01:CDS 25.0%
!!! AACCCTTTTAAGAGAAAAAA+CGG + Chr8:54662871-54662890 None:intergenic 25.0%
!!! AAGCTATTATTTTGACATCT+GGG - Chr8:54663100-54663119 MsG0880045134.01.T01:CDS 25.0%
!!! CCTATTTTAATAAACATTGG+TGG - Chr8:54662905-54662924 MsG0880045134.01.T01:CDS 25.0%
!!! GTATAATCATGCACATTTTT+TGG - Chr8:54663587-54663606 MsG0880045134.01.T01:CDS 25.0%
!!! TATGTACTCTTTTTCAGAAT+TGG - Chr8:54663958-54663977 MsG0880045134.01.T01:intron 25.0%
!!! TCCCCTATTTTAATAAACAT+TGG - Chr8:54662902-54662921 MsG0880045134.01.T01:CDS 25.0%
AATGTGCATGATTATACCAT+AGG + Chr8:54663584-54663603 None:intergenic 30.0%
ATGAGGTTAATAACTTGACA+TGG - Chr8:54663441-54663460 MsG0880045134.01.T01:CDS 30.0%
GAGATAACAAACATGTATTG+AGG + Chr8:54664017-54664036 None:intergenic 30.0%
TCCTTGAAGCGAATTATATT+CGG + Chr8:54663220-54663239 None:intergenic 30.0%
! CTGGAAAATACACTTTTAGA+TGG - Chr8:54663704-54663723 MsG0880045134.01.T01:intron 30.0%
! GACTTTATTTGTTTACCTGT+TGG - Chr8:54663246-54663265 MsG0880045134.01.T01:intron 30.0%
! GTTGTTAACTGTTACTTCAA+TGG - Chr8:54663305-54663324 MsG0880045134.01.T01:intron 30.0%
! TCTTGAGTGTTTCTTATTTG+TGG - Chr8:54663836-54663855 MsG0880045134.01.T01:intron 30.0%
! TGTTAACTGTTACTTCAATG+GGG - Chr8:54663307-54663326 MsG0880045134.01.T01:intron 30.0%
!!! AGACCGTTTTTTCTCTTAAA+AGG - Chr8:54662865-54662884 MsG0880045134.01.T01:CDS 30.0%
!!! GACCGTTTTTTCTCTTAAAA+GGG - Chr8:54662866-54662885 MsG0880045134.01.T01:CDS 30.0%
ATAGTAACACAATATGCAGC+TGG - Chr8:54663371-54663390 MsG0880045134.01.T01:CDS 35.0%
CCTTGAAGCGAATTATATTC+GGG + Chr8:54663219-54663238 None:intergenic 35.0%
GAATATAATTCGCTTCAAGG+AGG - Chr8:54663219-54663238 MsG0880045134.01.T01:intron 35.0%
TACAATTCATTCCAGTACCT+TGG + Chr8:54663771-54663790 None:intergenic 35.0%
TATCTAGCGTGGATAAACAA+AGG + Chr8:54663524-54663543 None:intergenic 35.0%
TCAATACAAACTGATCCAAC+AGG + Chr8:54663264-54663283 None:intergenic 35.0%
TCTCGACCTAAATCAATTGT+TGG - Chr8:54663879-54663898 MsG0880045134.01.T01:intron 35.0%
TGCTTGAAAATCTGTGACTT+CGG - Chr8:54663740-54663759 MsG0880045134.01.T01:intron 35.0%
TTATCAACCACAGATCTCTT+CGG - Chr8:54663191-54663210 MsG0880045134.01.T01:intron 35.0%
TTTCCAGATTGATGAGAATG+TGG - Chr8:54663159-54663178 MsG0880045134.01.T01:CDS 35.0%
TTTGCACAAATGCATTGAAG+CGG + Chr8:54662971-54662990 None:intergenic 35.0%
! CAATTGATTTAGGTCGAGAA+TGG + Chr8:54663878-54663897 None:intergenic 35.0%
! GGAGTTCCAACAATTGATTT+AGG + Chr8:54663888-54663907 None:intergenic 35.0%
!! AAGTCACAGATTTTCAAGCA+AGG + Chr8:54663740-54663759 None:intergenic 35.0%
!! TACTTCAATGGGGTTTTGAT+TGG - Chr8:54663317-54663336 MsG0880045134.01.T01:intron 35.0%
AAGCGGCCTAAAAAATTCGA+GGG + Chr8:54662954-54662973 None:intergenic 40.0%
CCCGAATATAATTCGCTTCA+AGG - Chr8:54663216-54663235 MsG0880045134.01.T01:intron 40.0%
GCTGAAAGAAATATCTAGCG+TGG + Chr8:54663535-54663554 None:intergenic 40.0%
GGCAAACACACAAAGATGTA+AGG + Chr8:54663996-54664015 None:intergenic 40.0%
TAACACAATATGCAGCTGGA+GGG - Chr8:54663375-54663394 MsG0880045134.01.T01:CDS 40.0%
TAATTCGCTTCAAGGAGGTA+AGG - Chr8:54663224-54663243 MsG0880045134.01.T01:intron 40.0%
TAGCCACATTCTCATCAATC+TGG + Chr8:54663165-54663184 None:intergenic 40.0%
TGGTGTCTATTACTGTCCTA+TGG - Chr8:54663565-54663584 MsG0880045134.01.T01:intron 40.0%
TGTTGGAACTCCAGCTTATA+TGG - Chr8:54663896-54663915 MsG0880045134.01.T01:intron 40.0%
TTCTTTCAGCAGCTGATATC+TGG - Chr8:54663545-54663564 MsG0880045134.01.T01:intron 40.0%
TTGCCTTGAATACCGATACT+CGG - Chr8:54662798-54662817 MsG0880045134.01.T01:CDS 40.0%
TTTCCAGCTTCAAATCTCTG+TGG + Chr8:54663691-54663710 None:intergenic 40.0%
! ACTAGAGGTTCTTTCTAGGA+GGG - Chr8:54663920-54663939 MsG0880045134.01.T01:intron 40.0%
!! AGATTTTCAAGCAAGGTGCA+GGG + Chr8:54663733-54663752 None:intergenic 40.0%
!! ATACCGAGTATCGGTATTCA+AGG + Chr8:54662804-54662823 None:intergenic 40.0%
AACACAATATGCAGCTGGAG+GGG - Chr8:54663376-54663395 MsG0880045134.01.T01:CDS 45.0%
ACCTCGAGATTATTCTAGCC+AGG + Chr8:54662837-54662856 None:intergenic 45.0%
ACCTCTAGTGCCATATAAGC+TGG + Chr8:54663909-54663928 None:intergenic 45.0%
CACTAGAGGTTCTTTCTAGG+AGG - Chr8:54663919-54663938 MsG0880045134.01.T01:intron 45.0%
CTGTGACTTCGGTTATTCCA+AGG - Chr8:54663751-54663770 MsG0880045134.01.T01:intron 45.0%
CTTCGGTTATTCCAAGGTAC+TGG - Chr8:54663757-54663776 MsG0880045134.01.T01:intron 45.0%
GAAGCGGCCTAAAAAATTCG+AGG + Chr8:54662955-54662974 None:intergenic 45.0%
GGAGCATGAATACCGAGTAT+CGG + Chr8:54662813-54662832 None:intergenic 45.0%
GTAACACAATATGCAGCTGG+AGG - Chr8:54663374-54663393 MsG0880045134.01.T01:CDS 45.0%
TCCAGCTTATATGGCACTAG+AGG - Chr8:54663905-54663924 MsG0880045134.01.T01:intron 45.0%
TCCTGGCTAGAATAATCTCG+AGG - Chr8:54662833-54662852 MsG0880045134.01.T01:CDS 45.0%
TCGAGATTATTCTAGCCAGG+AGG + Chr8:54662834-54662853 None:intergenic 45.0%
TGGCACTAGAGGTTCTTTCT+AGG - Chr8:54663916-54663935 MsG0880045134.01.T01:intron 45.0%
TTTGAGAGGACCTGTAATGC+TGG - Chr8:54663404-54663423 MsG0880045134.01.T01:CDS 45.0%
! ATGCCACAGAGATTTGAAGC+TGG - Chr8:54663685-54663704 MsG0880045134.01.T01:intron 45.0%
! CTTTCTAGGAGGGAGTATGA+TGG - Chr8:54663930-54663949 MsG0880045134.01.T01:intron 45.0%
!! CAGATTTTCAAGCAAGGTGC+AGG + Chr8:54663734-54663753 None:intergenic 45.0%
!!! CAATGGGGTTTTGATTGGTC+AGG - Chr8:54663322-54663341 MsG0880045134.01.T01:intron 45.0%
!!! GAGCAGCCCTCGAATTTTTT+AGG - Chr8:54662945-54662964 MsG0880045134.01.T01:intron 45.0%
AAGCTTCACCAGAGGCTTGA+TGG + Chr8:54662764-54662783 None:intergenic 50.0%
AGAAAGCCAAGCTTCACCAG+AGG + Chr8:54662772-54662791 None:intergenic 50.0%
AGAGGACCTGTAATGCTGGT+CGG - Chr8:54663408-54663427 MsG0880045134.01.T01:CDS 50.0%
ATCAAGCCTCTGGTGAAGCT+TGG - Chr8:54662763-54662782 MsG0880045134.01.T01:CDS 50.0%
GAATTGCACCATCAAGCCTC+TGG - Chr8:54662753-54662772 MsG0880045134.01.T01:CDS 50.0%
! TAGGAGGGAGTATGATGGCA+TGG - Chr8:54663935-54663954 MsG0880045134.01.T01:intron 50.0%
!!! TGGGGTTTTGATTGGTCAGG+TGG - Chr8:54663325-54663344 MsG0880045134.01.T01:intron 50.0%
CTCGGTATTCATGCTCCTCC+TGG - Chr8:54662816-54662835 MsG0880045134.01.T01:CDS 55.0%
CTGGAGGGGAACTCTTTGAG+AGG - Chr8:54663390-54663409 MsG0880045134.01.T01:CDS 55.0%
GCTGAACCGACCAGCATTAC+AGG + Chr8:54663417-54663436 None:intergenic 55.0%
! GGTGAAGCTTGGCTTTCTCG+AGG - Chr8:54662774-54662793 MsG0880045134.01.T01:CDS 55.0%
! TGGTCGGTTCAGCGAAGATG+AGG - Chr8:54663424-54663443 MsG0880045134.01.T01:CDS 55.0%
!! TCGGGTGCCGAAGAGATCTG+TGG + Chr8:54663201-54663220 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 54662751 54664061 54662751 ID=MsG0880045134.01;Name=MsG0880045134.01
Chr8 mRNA 54662751 54664061 54662751 ID=MsG0880045134.01.T01;Parent=MsG0880045134.01;Name=MsG0880045134.01.T01;_AED=0.48;_eAED=0.59;_QI=0|0|0|1|0|0|5|0|203
Chr8 exon 54663993 54664061 54663993 ID=MsG0880045134.01.T01:exon:23599;Parent=MsG0880045134.01.T01
Chr8 exon 54663572 54663646 54663572 ID=MsG0880045134.01.T01:exon:23598;Parent=MsG0880045134.01.T01
Chr8 exon 54663350 54663468 54663350 ID=MsG0880045134.01.T01:exon:23597;Parent=MsG0880045134.01.T01
Chr8 exon 54663040 54663190 54663040 ID=MsG0880045134.01.T01:exon:23596;Parent=MsG0880045134.01.T01
Chr8 exon 54662751 54662948 54662751 ID=MsG0880045134.01.T01:exon:23595;Parent=MsG0880045134.01.T01
Chr8 CDS 54663993 54664061 54663993 ID=MsG0880045134.01.T01:cds;Parent=MsG0880045134.01.T01
Chr8 CDS 54663572 54663646 54663572 ID=MsG0880045134.01.T01:cds;Parent=MsG0880045134.01.T01
Chr8 CDS 54663350 54663468 54663350 ID=MsG0880045134.01.T01:cds;Parent=MsG0880045134.01.T01
Chr8 CDS 54663040 54663190 54663040 ID=MsG0880045134.01.T01:cds;Parent=MsG0880045134.01.T01
Chr8 CDS 54662751 54662948 54662751 ID=MsG0880045134.01.T01:cds;Parent=MsG0880045134.01.T01
Gene Sequence

>MsG0880045134.01.T01

ATGAATTGCACCATCAAGCCTCTGGTGAAGCTTGGCTTTCTCGAGGTTTGCCTTGAATACCGATACTCGATTGATGAGAATGTGGCTAGCGAGATTATCAACCACAGATCTCTTCGGCACCCGAATATAATTCGCTTCAAGGAGGTGGTTTTGACCCCTAATCATTTAGCAATAGTAACACAATATGCAGCTGGAGGGGAACTCTTTGAGAGGACCTGTAATGCTGGTCGGTTCAGCGAAGATGAGGTTAATAACTTGACATGGACTCTGTCTTCAAAAGTTCGCAAAGTGAGAACTTATGTTTTTACTCTTCTTTTGCAGCAAATATGCCACAGAGATTTGAAGCTGGAAAATACACTTTTAGATGGAAGCCCTGCACCTTGCTTGAAAATCTGTGACTTCGGTTATTCCAAGTCAGCTTTGCTCCATTCTCGACCTAAATCAATTGTTGGAACTCCAGCTTATATGGCACTAGAGGTTCTTTCTAGGAGGGAGTATGATGGCATGGTATGTACTCTTTTTCAGAATTGGTTCTGCATAGTTCCTTACATCTTTGTGTGTTTGCCTCAATACATGTTTGTTATCTCTAATTTTATAGCTATATTCATTTGA
Protein sequence

>MsG0880045134.01.T01

MNCTIKPLVKLGFLEVCLEYRYSIDENVASEIINHRSLRHPNIIRFKEVVLTPNHLAIVTQYAAGGELFERTCNAGRFSEDEVNNLTWTLSSKVRKVRTYVFTLLLQQICHRDLKLENTLLDGSPAPCLKICDFGYSKSALLHSRPKSIVGTPAYMALEVLSRREYDGMVCTLFQNWFCIVPYIFVCLPQYMFVISNFIAIFI*