AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045862.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045862.01.T01 MTR_3g101520 48.454 97 50 0 110 206 98 194 4.08e-25 99.4
MsG0880045862.01.T01 MTR_3g101520 48.454 97 50 0 110 206 106 202 7.17e-25 99.0
MsG0880045862.01.T01 MTR_2g102450 59.211 76 31 0 124 199 74 149 2.48e-24 99.8
MsG0880045862.01.T01 MTR_2g102450 59.211 76 31 0 124 199 128 203 3.24e-24 100
MsG0880045862.01.T01 MTR_4g008310 46.250 80 43 0 120 199 199 278 8.16e-17 78.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045862.01.T01 AT3G19184 45.361 97 53 0 110 206 102 198 1.36e-23 96.7
MsG0880045862.01.T01 AT3G19184 45.361 97 53 0 110 206 102 198 1.37e-23 96.7
MsG0880045862.01.T01 AT5G42700 54.023 87 36 1 113 199 64 146 5.81e-23 92.8
MsG0880045862.01.T01 AT5G42700 54.023 87 36 1 113 199 109 191 1.19e-22 93.2
MsG0880045862.01.T01 AT5G58280 48.684 76 39 0 124 199 113 188 6.31e-14 70.1
MsG0880045862.01.T01 AT5G58280 48.684 76 39 0 124 199 146 221 8.66e-14 70.1
MsG0880045862.01.T01 AT5G58280 48.684 76 39 0 124 199 145 220 8.85e-14 70.1

Find 38 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGAGAAAACAACATTTAA+AGG 0.226352 8:-65595584 None:intergenic
TTCACAAAACTAGGGAATTC+TGG 0.261063 8:-65596627 None:intergenic
TAAAAGACATTTACCTGATA+AGG 0.319627 8:+65596876 MsG0880045862.01.T01:CDS
GTGATAGTTGTATCCTTATC+AGG 0.331030 8:-65596889 None:intergenic
AATACAACAAATTAAAAGTA+TGG 0.346760 8:-65597226 None:intergenic
ACAACATTTAAAGGAGACTT+TGG 0.371950 8:-65595575 None:intergenic
TTGATTGCAGTTCAACTAAA+GGG 0.392484 8:+65596523 MsG0880045862.01.T01:intron
TTATGGGAGAGAGGTGTATG+AGG 0.397309 8:+65595529 MsG0880045862.01.T01:CDS
CGAAGGACTTCGATCACATT+TGG 0.418810 8:+65596602 MsG0880045862.01.T01:CDS
ATCACATTAGAAGATGAATA+TGG 0.425783 8:+65596907 MsG0880045862.01.T01:CDS
TTTGATTGCAGTTCAACTAA+AGG 0.434875 8:+65596522 MsG0880045862.01.T01:intron
TTTGTGATTGTGTCTCATCT+AGG 0.440432 8:-65596402 None:intergenic
TTTGGAGAGAAATGTGTCAT+AGG 0.464884 8:-65595557 None:intergenic
TTGTGATTGTGTCTCATCTA+GGG 0.479490 8:-65596401 None:intergenic
CACAATCACAAAAGTTTGCA+AGG 0.496554 8:+65596413 MsG0880045862.01.T01:CDS
TTTCCACGAAATGATGATCA+TGG 0.525235 8:+65595491 MsG0880045862.01.T01:CDS
AGCAAGGACTTCACAAAACT+AGG 0.525979 8:-65596636 None:intergenic
AAATACATTGCATGTAAGAC+AGG 0.526557 8:+65596946 MsG0880045862.01.T01:CDS
CAAATGTGATCGAAGTCCTT+CGG 0.541447 8:-65596601 None:intergenic
TATGGGAGAGAGGTGTATGA+GGG 0.546719 8:+65595530 MsG0880045862.01.T01:CDS
ATTAATGTCGAAAATGGTGG+CGG 0.572487 8:+65595421 None:intergenic
GCAAGGACTTCACAAAACTA+GGG 0.574234 8:-65596635 None:intergenic
TCAACTAAAGGGGTAACAGA+TGG 0.578200 8:+65596534 MsG0880045862.01.T01:CDS
GTATAAACAGAACAAGGCGG+TGG 0.586932 8:+65595815 MsG0880045862.01.T01:intron
TCTGCTGTGATTCACGCCGA+AGG 0.597081 8:+65596585 MsG0880045862.01.T01:CDS
TGGCGGTTGATGAAAAGAAG+AGG 0.598421 8:+65595438 MsG0880045862.01.T01:CDS
ACTCCATGATCATCATTTCG+TGG 0.602909 8:-65595494 None:intergenic
TGATTGCAGTTCAACTAAAG+GGG 0.603233 8:+65596524 MsG0880045862.01.T01:intron
TGAAAAGAAGAGGGATCGAA+TGG 0.606536 8:+65595448 MsG0880045862.01.T01:CDS
AGCTACATGTGATCTGAGCA+AGG 0.622819 8:-65596652 None:intergenic
GGCGGTTGATGAAAAGAAGA+GGG 0.624988 8:+65595439 MsG0880045862.01.T01:CDS
TTAGGTTTATATAATAAGAG+CGG 0.625618 8:+65597146 MsG0880045862.01.T01:CDS
AGACAGGATTGAGTGCTGGT+TGG 0.629436 8:+65596962 MsG0880045862.01.T01:CDS
TGTAAGACAGGATTGAGTGC+TGG 0.630499 8:+65596958 MsG0880045862.01.T01:CDS
TTTGCAGTATAAACAGAACA+AGG 0.647255 8:+65595809 MsG0880045862.01.T01:intron
TGGAACATATTCTCGTGGCG+AGG 0.659835 8:+65596554 MsG0880045862.01.T01:CDS
ACAGATGGAACATATTCTCG+TGG 0.680750 8:+65596549 MsG0880045862.01.T01:CDS
GCAGTATAAACAGAACAAGG+CGG 0.787127 8:+65595812 MsG0880045862.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTAATTGTCATTTTATT+AGG - Chr8:65596768-65596787 None:intergenic 10.0%
!!! TCTTTTATTAGTTTTAATTT+TGG + Chr8:65597101-65597120 MsG0880045862.01.T01:intron 10.0%
!! AAAAACTATTACCTTAAATT+TGG - Chr8:65597054-65597073 None:intergenic 15.0%
!! AAATTAAATCAAAGTCAAAA+TGG + Chr8:65596343-65596362 MsG0880045862.01.T01:intron 15.0%
!! AATACAACAAATTAAAAGTA+TGG - Chr8:65597229-65597248 None:intergenic 15.0%
!! ACATGTAAAAAAATGTAAAA+TGG - Chr8:65596150-65596169 None:intergenic 15.0%
!! AGAATTTAACATTCAATTAA+TGG - Chr8:65596319-65596338 None:intergenic 15.0%
!! GAATTTAACATTCAATTAAT+GGG - Chr8:65596318-65596337 None:intergenic 15.0%
!! TAAAAAAATGTAAAATGGAT+GGG - Chr8:65596145-65596164 None:intergenic 15.0%
!! TTAGTTATATTCAAATTTAG+AGG - Chr8:65596726-65596745 None:intergenic 15.0%
!!! AATTTTATTGATAATGTTTC+TGG + Chr8:65595712-65595731 MsG0880045862.01.T01:intron 15.0%
!!! TACTTACTTTAAAAATAAGT+TGG - Chr8:65596473-65596492 None:intergenic 15.0%
!!! TTCTAATATTGAAAACATTT+TGG + Chr8:65596184-65596203 MsG0880045862.01.T01:intron 15.0%
!! AAAAAAATGTAAAATGGATG+GGG - Chr8:65596144-65596163 None:intergenic 20.0%
!! GTAAAAAAATGTAAAATGGA+TGG - Chr8:65596146-65596165 None:intergenic 20.0%
!! TTAGGTTTATATAATAAGAG+CGG + Chr8:65597146-65597165 MsG0880045862.01.T01:CDS 20.0%
!! TTTACATGTATTGAAAACAT+TGG + Chr8:65596161-65596180 MsG0880045862.01.T01:intron 20.0%
!!! AAAACAAGTAAGATTTTGAA+GGG + Chr8:65595869-65595888 MsG0880045862.01.T01:intron 20.0%
!!! TTACTCATATCTTTTTTCTT+AGG + Chr8:65597128-65597147 MsG0880045862.01.T01:CDS 20.0%
!!! TTGTGATTTCTTTTTGTATA+GGG + Chr8:65596835-65596854 MsG0880045862.01.T01:intron 20.0%
! AAAAAATGTAAAATGGATGG+GGG - Chr8:65596143-65596162 None:intergenic 25.0%
! AACCAAACAATTAGATGAAA+GGG - Chr8:65596238-65596257 None:intergenic 25.0%
! AACTATTACCTTAAATTTGG+TGG - Chr8:65597051-65597070 None:intergenic 25.0%
! ACATAATAATGTGTGTTTGA+TGG + Chr8:65595677-65595696 MsG0880045862.01.T01:intron 25.0%
! ACTATTACCTTAAATTTGGT+GGG - Chr8:65597050-65597069 None:intergenic 25.0%
! ATCACATTAGAAGATGAATA+TGG + Chr8:65596907-65596926 MsG0880045862.01.T01:CDS 25.0%
! CATAATAATGTGTGTTTGAT+GGG + Chr8:65595678-65595697 MsG0880045862.01.T01:intron 25.0%
! GAAGAGAAAACAACATTTAA+AGG - Chr8:65595587-65595606 None:intergenic 25.0%
! TAAAAGACATTTACCTGATA+AGG + Chr8:65596876-65596895 MsG0880045862.01.T01:CDS 25.0%
! TAAAATGAAGCAACTGTATT+AGG + Chr8:65596782-65596801 MsG0880045862.01.T01:intron 25.0%
! TCATAGAAGGTAAAATTATG+TGG - Chr8:65596211-65596230 None:intergenic 25.0%
! TTAAATCTCAATAGGTTACT+CGG + Chr8:65595966-65595985 MsG0880045862.01.T01:intron 25.0%
! TTTACACTTTGTGAAAAATG+AGG - Chr8:65595623-65595642 None:intergenic 25.0%
!! CAAAACAAGTAAGATTTTGA+AGG + Chr8:65595868-65595887 MsG0880045862.01.T01:intron 25.0%
!! CTTGTGATTTCTTTTTGTAT+AGG + Chr8:65596834-65596853 MsG0880045862.01.T01:intron 25.0%
!! TGTGATTTCTTTTTGTATAG+GGG + Chr8:65596836-65596855 MsG0880045862.01.T01:intron 25.0%
!!! CAAAATCTTACTTGTTTTGT+TGG - Chr8:65595867-65595886 None:intergenic 25.0%
AAATACATTGCATGTAAGAC+AGG + Chr8:65596946-65596965 MsG0880045862.01.T01:CDS 30.0%
AATGGTGAAAAAAGATGTGA+TGG + Chr8:65595466-65595485 MsG0880045862.01.T01:CDS 30.0%
AATTGAACCCACCAAATTTA+AGG + Chr8:65597040-65597059 MsG0880045862.01.T01:intron 30.0%
ACAACATTTAAAGGAGACTT+TGG - Chr8:65595578-65595597 None:intergenic 30.0%
CAAAAAGAAATCACAAGACA+TGG - Chr8:65596831-65596850 None:intergenic 30.0%
CAACCAAACAATTAGATGAA+AGG - Chr8:65596239-65596258 None:intergenic 30.0%
TACTAAACAATGTATCACAG+AGG + Chr8:65596027-65596046 MsG0880045862.01.T01:intron 30.0%
TCAATCCAGATTGTGAATAA+CGG + Chr8:65595935-65595954 MsG0880045862.01.T01:intron 30.0%
TTGATTGCAGTTCAACTAAA+GGG + Chr8:65596523-65596542 MsG0880045862.01.T01:intron 30.0%
TTTGATTGCAGTTCAACTAA+AGG + Chr8:65596522-65596541 MsG0880045862.01.T01:intron 30.0%
TTTGCAGTATAAACAGAACA+AGG + Chr8:65595809-65595828 MsG0880045862.01.T01:intron 30.0%
! AGGCTTGTTTAAATCTCAAT+AGG + Chr8:65595958-65595977 MsG0880045862.01.T01:intron 30.0%
AGTTGCACCAAACAAAAAGT+GGG + Chr8:65597186-65597205 MsG0880045862.01.T01:CDS 35.0%
ATAATTGCTAGAAGGTGATG+TGG + Chr8:65597004-65597023 MsG0880045862.01.T01:intron 35.0%
ATCACAAAAGTTTGCAAGGT+TGG + Chr8:65596417-65596436 MsG0880045862.01.T01:intron 35.0%
CAAAAAGAAGCCAAACCTAT+AGG - Chr8:65595747-65595766 None:intergenic 35.0%
CACAATCACAAAAGTTTGCA+AGG + Chr8:65596413-65596432 MsG0880045862.01.T01:CDS 35.0%
CACCCTTTCATCTAATTGTT+TGG + Chr8:65596233-65596252 MsG0880045862.01.T01:intron 35.0%
CTAACATATGCATTTCCTAC+AGG + Chr8:65596375-65596394 MsG0880045862.01.T01:intron 35.0%
GTGATAGTTGTATCCTTATC+AGG - Chr8:65596892-65596911 None:intergenic 35.0%
TGATTGCAGTTCAACTAAAG+GGG + Chr8:65596524-65596543 MsG0880045862.01.T01:intron 35.0%
TTCACAAAACTAGGGAATTC+TGG - Chr8:65596630-65596649 None:intergenic 35.0%
TTGTGATTGTGTCTCATCTA+GGG - Chr8:65596404-65596423 None:intergenic 35.0%
TTTCCACGAAATGATGATCA+TGG + Chr8:65595491-65595510 MsG0880045862.01.T01:CDS 35.0%
TTTGGAGAGAAATGTGTCAT+AGG - Chr8:65595560-65595579 None:intergenic 35.0%
TTTGTGATTGTGTCTCATCT+AGG - Chr8:65596405-65596424 None:intergenic 35.0%
! GAGTGACTGTTGCATTTTAT+GGG + Chr8:65595513-65595532 MsG0880045862.01.T01:CDS 35.0%
! GCTTTAGATTCCTATAGGTT+TGG + Chr8:65595734-65595753 MsG0880045862.01.T01:intron 35.0%
! TAAGCTTGTAAAGCACTACA+AGG - Chr8:65596293-65596312 None:intergenic 35.0%
! TGTCTTTTACAAAACGACAC+AGG - Chr8:65596865-65596884 None:intergenic 35.0%
!! ATGAAAGGGTGTTTCATAGA+AGG - Chr8:65596224-65596243 None:intergenic 35.0%
!! ATTTGGTGGGTTCAATTAGT+TGG - Chr8:65597037-65597056 None:intergenic 35.0%
!! CACATGTAGCTAGTTGTTTT+TGG + Chr8:65596664-65596683 MsG0880045862.01.T01:CDS 35.0%
!! CTATAGGTTTGGCTTCTTTT+TGG + Chr8:65595745-65595764 MsG0880045862.01.T01:intron 35.0%
!! TTCTGGCTTTAGATTCCTAT+AGG + Chr8:65595729-65595748 MsG0880045862.01.T01:intron 35.0%
!!! TGTAGCTAGTTGTTTTTGGA+TGG + Chr8:65596668-65596687 MsG0880045862.01.T01:CDS 35.0%
AAATGTAAAATGGATGGGGG+TGG - Chr8:65596140-65596159 None:intergenic 40.0%
AATGTAAAATGGATGGGGGT+GGG - Chr8:65596139-65596158 None:intergenic 40.0%
ACAGATGGAACATATTCTCG+TGG + Chr8:65596549-65596568 MsG0880045862.01.T01:CDS 40.0%
ACTCCATGATCATCATTTCG+TGG - Chr8:65595497-65595516 None:intergenic 40.0%
AGCAAGGACTTCACAAAACT+AGG - Chr8:65596639-65596658 None:intergenic 40.0%
ATCCAGATTGTGAATAACGG+AGG + Chr8:65595938-65595957 MsG0880045862.01.T01:intron 40.0%
CAAATGTGATCGAAGTCCTT+CGG - Chr8:65596604-65596623 None:intergenic 40.0%
CAACCAGTGTTGTCAACTAT+CGG - Chr8:65596104-65596123 None:intergenic 40.0%
CAAGACATGGCGATGTTATT+TGG - Chr8:65596818-65596837 None:intergenic 40.0%
GAGTTGCACCAAACAAAAAG+TGG + Chr8:65597185-65597204 MsG0880045862.01.T01:CDS 40.0%
GCAAGGACTTCACAAAACTA+GGG - Chr8:65596638-65596657 None:intergenic 40.0%
GCAGTATAAACAGAACAAGG+CGG + Chr8:65595812-65595831 MsG0880045862.01.T01:intron 40.0%
TCAACTAAAGGGGTAACAGA+TGG + Chr8:65596534-65596553 MsG0880045862.01.T01:CDS 40.0%
TGAAAAGAAGAGGGATCGAA+TGG + Chr8:65595448-65595467 MsG0880045862.01.T01:CDS 40.0%
TGCTGTGCATAATTGCTAGA+AGG + Chr8:65596996-65597015 MsG0880045862.01.T01:intron 40.0%
! GGAGTGACTGTTGCATTTTA+TGG + Chr8:65595512-65595531 MsG0880045862.01.T01:CDS 40.0%
! TGTTGCATTTTATGGGAGAG+AGG + Chr8:65595520-65595539 MsG0880045862.01.T01:CDS 40.0%
AGCCTCCGTTATTCACAATC+TGG - Chr8:65595943-65595962 None:intergenic 45.0%
AGCTACATGTGATCTGAGCA+AGG - Chr8:65596655-65596674 None:intergenic 45.0%
ATTGCTAGAAGGTGATGTGG+TGG + Chr8:65597007-65597026 MsG0880045862.01.T01:intron 45.0%
CGAAGGACTTCGATCACATT+TGG + Chr8:65596602-65596621 MsG0880045862.01.T01:CDS 45.0%
GTATAAACAGAACAAGGCGG+TGG + Chr8:65595815-65595834 MsG0880045862.01.T01:intron 45.0%
TAGCCGATAGTTGACAACAC+TGG + Chr8:65596098-65596117 MsG0880045862.01.T01:intron 45.0%
TGCACCAAACAAAAAGTGGG+AGG + Chr8:65597189-65597208 MsG0880045862.01.T01:CDS 45.0%
TGTAAGACAGGATTGAGTGC+TGG + Chr8:65596958-65596977 MsG0880045862.01.T01:CDS 45.0%
! GCTTGTAAAGCACTACAAGG+TGG - Chr8:65596290-65596309 None:intergenic 45.0%
! TATGGGAGAGAGGTGTATGA+GGG + Chr8:65595530-65595549 MsG0880045862.01.T01:CDS 45.0%
! TTATGGGAGAGAGGTGTATG+AGG + Chr8:65595529-65595548 MsG0880045862.01.T01:CDS 45.0%
!! GGCGGTTGATGAAAAGAAGA+GGG + Chr8:65595439-65595458 MsG0880045862.01.T01:CDS 45.0%
!! TGGCGGTTGATGAAAAGAAG+AGG + Chr8:65595438-65595457 MsG0880045862.01.T01:CDS 45.0%
!!! CTTGCCTCCCACTTTTTGTT+TGG - Chr8:65597196-65597215 None:intergenic 45.0%
TGGAACATATTCTCGTGGCG+AGG + Chr8:65596554-65596573 MsG0880045862.01.T01:CDS 50.0%
! TCTCATCTAGGGTTGCCTGT+AGG - Chr8:65596393-65596412 None:intergenic 50.0%
!! AGACAGGATTGAGTGCTGGT+TGG + Chr8:65596962-65596981 MsG0880045862.01.T01:CDS 50.0%
TCTGCTGTGATTCACGCCGA+AGG + Chr8:65596585-65596604 MsG0880045862.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 65595434 65597262 65595434 ID=MsG0880045862.01;Name=MsG0880045862.01
Chr8 mRNA 65595434 65597262 65595434 ID=MsG0880045862.01.T01;Parent=MsG0880045862.01;Name=MsG0880045862.01.T01;_AED=0.51;_eAED=0.61;_QI=0|0.6|0.5|1|0.4|0.5|6|0|247
Chr8 exon 65595434 65595637 65595434 ID=MsG0880045862.01.T01:exon:24975;Parent=MsG0880045862.01.T01
Chr8 exon 65595816 65595875 65595816 ID=MsG0880045862.01.T01:exon:24976;Parent=MsG0880045862.01.T01
Chr8 exon 65596397 65596434 65596397 ID=MsG0880045862.01.T01:exon:24977;Parent=MsG0880045862.01.T01
Chr8 exon 65596533 65596689 65596533 ID=MsG0880045862.01.T01:exon:24978;Parent=MsG0880045862.01.T01
Chr8 exon 65596856 65596989 65596856 ID=MsG0880045862.01.T01:exon:24979;Parent=MsG0880045862.01.T01
Chr8 exon 65597112 65597262 65597112 ID=MsG0880045862.01.T01:exon:24980;Parent=MsG0880045862.01.T01
Chr8 CDS 65595434 65595637 65595434 ID=MsG0880045862.01.T01:cds;Parent=MsG0880045862.01.T01
Chr8 CDS 65595816 65595875 65595816 ID=MsG0880045862.01.T01:cds;Parent=MsG0880045862.01.T01
Chr8 CDS 65596397 65596434 65596397 ID=MsG0880045862.01.T01:cds;Parent=MsG0880045862.01.T01
Chr8 CDS 65596533 65596689 65596533 ID=MsG0880045862.01.T01:cds;Parent=MsG0880045862.01.T01
Chr8 CDS 65596856 65596989 65596856 ID=MsG0880045862.01.T01:cds;Parent=MsG0880045862.01.T01
Chr8 CDS 65597112 65597262 65597112 ID=MsG0880045862.01.T01:cds;Parent=MsG0880045862.01.T01
Gene Sequence

>MsG0880045862.01.T01

ATGGTGGCGGTTGATGAAAAGAAGAGGGATCGAATGGTGAAAAAAGATGTGATGGAATTTCCACGAAATGATGATCATGGAGTGACTGTTGCATTTTATGGGAGAGAGGTGTATGAGGGTGTTCCTATGACACATTTCTCTCCAAAGTCTCCTTTAAATGTTGTTTTCTCTTCTCTATTTACATATCCTCATTTTTCACAAAGTTATAAACAGAACAAGGCGGTGGAAAATAAATACAAGCTTTTGAATAGTCCAACAAAACAAGCAACCCTAGATGAGACACAATCACAAAAGTTTGCAAGTTCAACTAAAGGGGTAACAGATGGAACATATTCTCGTGGCGAGGCGATATCATCTGCTGTGATTCACGCCGAAGGACTTCGATCACATTTGGAACCAGAATTCCCTAGTTTTGTGAAGTCCTTGCTCAGATCACATGTAGCTAGTTGTTTTTGGATGGGGTTGCCTGTGTCGTTTTGTAAAAGACATTTACCTGATAAGGATACAACTATCACATTAGAAGATGAATATGGTAAAGAATACAAAACAAAATACATTGCATGTAAGACAGGATTGAGTGCTGGTTGGAGACATTTTAATTTTGGTTCTTTACTCATATCTTTTTTCTTAGGTTTATATAATAAGAGCGGAACTAGAGAATCAAGAGAGTTGCACCAAACAAAAAGTGGGAGGCAAGACTTGCTTCTCCATACTTTTAATTTGTTGTATTATTGTCTATCATGA
Protein sequence

>MsG0880045862.01.T01

MVAVDEKKRDRMVKKDVMEFPRNDDHGVTVAFYGREVYEGVPMTHFSPKSPLNVVFSSLFTYPHFSQSYKQNKAVENKYKLLNSPTKQATLDETQSQKFASSTKGVTDGTYSRGEAISSAVIHAEGLRSHLEPEFPSFVKSLLRSHVASCFWMGLPVSFCKRHLPDKDTTITLEDEYGKEYKTKYIACKTGLSAGWRHFNFGSLLISFFLGLYNKSGTRESRELHQTKSGRQDLLLHTFNLLYYCLS*