AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045870.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045870.01.T01 MTR_5g017210 70.000 170 36 5 74 242 92 247 1.54e-62 196
MsG0880045870.01.T01 MTR_3g101810 53.670 218 74 6 10 205 40 252 1.66e-62 197
MsG0880045870.01.T01 MTR_8g093500 75.824 91 22 0 97 187 87 177 2.69e-46 155
MsG0880045870.01.T01 MTR_2g100490 52.027 148 62 4 80 218 242 389 1.25e-29 114
MsG0880045870.01.T01 MTR_8g015450 59.596 99 36 1 96 190 296 394 2.04e-27 108
MsG0880045870.01.T01 MTR_7g069590 66.667 60 20 0 130 189 32 91 5.60e-23 91.7
MsG0880045870.01.T01 MTR_5g079500 54.430 79 34 1 110 186 202 280 9.00e-20 86.7
MsG0880045870.01.T01 MTR_1g106460 52.239 67 32 0 120 186 234 300 3.78e-17 79.7
MsG0880045870.01.T01 MTR_1g018090 60.714 56 22 0 131 186 281 336 4.37e-17 79.7
MsG0880045870.01.T01 MTR_5g005110 62.963 54 20 0 133 186 233 286 6.44e-17 79.0
MsG0880045870.01.T01 MTR_1g106470 60.714 56 22 0 131 186 203 258 1.44e-16 77.4
MsG0880045870.01.T01 MTR_3g103030 55.556 63 28 0 124 186 229 291 4.12e-16 76.6
MsG0880045870.01.T01 MTR_1g080890 47.059 85 37 1 117 201 186 262 3.03e-15 74.3
MsG0880045870.01.T01 MTR_4g131160 52.778 72 26 1 131 202 206 269 7.95e-15 73.6
MsG0880045870.01.T01 MTR_4g131160 52.778 72 26 1 131 202 203 266 8.03e-15 73.6
MsG0880045870.01.T01 MTR_1g106480 64.444 45 16 0 142 186 87 131 1.60e-13 66.6
MsG0880045870.01.T01 MTR_2g067000 48.000 75 32 1 120 187 146 220 5.14e-13 67.4
MsG0880045870.01.T01 MTR_1g060700 57.407 54 23 0 134 187 357 410 3.10e-12 65.9
MsG0880045870.01.T01 MTR_4g033295 57.143 56 23 1 134 188 128 183 6.73e-12 62.8
MsG0880045870.01.T01 MTR_4g033295 57.407 54 22 1 134 186 128 181 1.81e-11 61.6
MsG0880045870.01.T01 MTR_2g089070 48.148 54 28 0 134 187 146 199 7.08e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045870.01.T01 AT5G67060 58.376 197 58 7 2 188 4 186 1.35e-50 165
MsG0880045870.01.T01 AT3G50330 55.769 208 67 7 6 205 11 201 1.40e-50 165
MsG0880045870.01.T01 AT5G67060 58.511 188 55 6 10 188 1 174 1.29e-48 160
MsG0880045870.01.T01 AT4G00120 68.750 96 27 1 99 191 87 182 1.95e-39 135
MsG0880045870.01.T01 AT3G21330 64.894 94 33 0 96 189 241 334 2.25e-36 132
MsG0880045870.01.T01 AT5G09750 76.667 90 21 0 97 186 92 181 9.98e-35 124
MsG0880045870.01.T01 AT5G01305 69.492 59 18 0 125 183 38 96 6.89e-22 89.4
MsG0880045870.01.T01 AT5G37800 35.542 166 78 5 24 186 124 263 1.77e-18 83.2
MsG0880045870.01.T01 AT5G43175 50.725 69 34 0 118 186 126 194 2.36e-18 81.3
MsG0880045870.01.T01 AT4G33880 59.677 62 25 0 125 186 267 328 2.89e-18 82.8
MsG0880045870.01.T01 AT1G66470 67.273 55 18 0 132 186 203 257 6.60e-18 81.3
MsG0880045870.01.T01 AT1G27740 52.941 68 32 0 119 186 162 229 6.95e-18 80.5
MsG0880045870.01.T01 AT2G14760 58.065 62 26 0 125 186 238 299 2.42e-17 80.1
MsG0880045870.01.T01 AT2G24260 41.071 112 47 3 122 215 28 138 1.30e-16 77.0
MsG0880045870.01.T01 AT2G24260 41.071 112 47 3 122 215 131 241 4.77e-16 76.6
MsG0880045870.01.T01 AT4G30980 41.584 101 51 1 120 220 126 218 1.03e-15 75.5
MsG0880045870.01.T01 AT4G30980 49.398 83 34 1 120 202 174 248 2.30e-15 74.7
MsG0880045870.01.T01 AT5G58010 47.674 86 36 2 121 205 96 173 1.27e-14 72.0
MsG0880045870.01.T01 AT2G14760 48.000 75 26 1 125 186 238 312 5.38e-14 70.9

Find 54 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTGAAGCAGTGTCCATTT+TGG 0.242900 8:-65725823 None:intergenic
AATAATGTGAATTATAATTC+TGG 0.269339 8:+65725981 MsG0880045870.01.T01:CDS
CTTCGGCGGCCTAACGGATT+CGG 0.285239 8:-65725691 None:intergenic
CACATTGGTGGTGGATTCAT+TGG 0.306846 8:+65725933 MsG0880045870.01.T01:CDS
AAAGGTTGATCAGAGAATTC+AGG 0.327420 8:-65725381 None:intergenic
CAGTGTTGTTGGTGATTTCT+TGG 0.347528 8:-65725425 None:intergenic
GTGGAGATGATAGTGCATTT+TGG 0.349089 8:-65725467 None:intergenic
GTGTAGCTGCTAGGCATAGA+AGG 0.352095 8:+65725748 MsG0880045870.01.T01:CDS
AAAGGATAAGTGAGAGGATA+AGG 0.357712 8:+65725772 MsG0880045870.01.T01:CDS
TTCGGCGGCCTAACGGATTC+GGG 0.378261 8:-65725690 None:intergenic
AGTACCTGGTGGAACCAAAA+TGG 0.383832 8:+65725809 MsG0880045870.01.T01:CDS
TAACTAGCTAAGCAATTGTT+TGG 0.384797 8:-65726051 None:intergenic
CCTTCTCTTCGGCGGCCTAA+CGG 0.391457 8:-65725697 None:intergenic
ATCTTCACATTCCTTCTCTT+CGG 0.392038 8:-65725708 None:intergenic
TGCCTAGCAGCTACACTTTG+TGG 0.400476 8:-65725741 None:intergenic
TCAATATTATTCAAACTTTG+TGG 0.420960 8:-65725486 None:intergenic
TAATTCTGGTTTGATGATGA+AGG 0.423318 8:+65725995 MsG0880045870.01.T01:CDS
AAAGAATTCGACTGCGAGTA+AGG 0.432337 8:-65725591 None:intergenic
GATTCGGGATCGATATGTAT+AGG 0.452777 8:-65725675 None:intergenic
AAGGTTGATCAGAGAATTCA+GGG 0.455273 8:-65725380 None:intergenic
GCTAAGCAATTGTTTGGAAG+TGG 0.457304 8:-65726045 None:intergenic
GTTGGTGATTTCTTGGTAAG+TGG 0.468287 8:-65725418 None:intergenic
CCGTTAGGCCGCCGAAGAGA+AGG 0.485491 8:+65725697 MsG0880045870.01.T01:CDS
AAATAATGGAACAGTGTTGT+TGG 0.485998 8:-65725436 None:intergenic
GATAGCAATGATGATGCAAA+TGG 0.487640 8:+65725350 MsG0880045870.01.T01:CDS
GTGGAACCCACCATTTGAAA+AGG 0.490966 8:-65726026 None:intergenic
GTGGTGGGAGTGTTATAAAA+AGG 0.493586 8:-65725399 None:intergenic
ATATCGATCCCGAATCCGTT+AGG 0.506193 8:+65725682 MsG0880045870.01.T01:CDS
GTGATTTCTTGGTAAGTGGT+GGG 0.510263 8:-65725414 None:intergenic
GTAAGGAAATGATGAAGAGT+TGG 0.515713 8:-65725574 None:intergenic
TTCACACGTTGGAACAAGTA+GGG 0.518979 8:+65725889 MsG0880045870.01.T01:CDS
GAATTCGATGGCAGCGATGA+GGG 0.520911 8:+65725623 MsG0880045870.01.T01:CDS
GGTGATTTCTTGGTAAGTGG+TGG 0.542766 8:-65725415 None:intergenic
GAAGAGTTGGTTTGAAGAGA+TGG 0.565464 8:-65725561 None:intergenic
TTCACATTCCTTCTCTTCGG+CGG 0.566092 8:-65725705 None:intergenic
ATTGGTGGTGGATTCATTGG+AGG 0.568963 8:+65725936 MsG0880045870.01.T01:CDS
GAAGGGAAAGGATAAGTGAG+AGG 0.571416 8:+65725766 MsG0880045870.01.T01:CDS
TTGGTGGTGGATTCATTGGA+GGG 0.580871 8:+65725937 MsG0880045870.01.T01:CDS
TTGACATTGGAGTGTAGAAG+TGG 0.581516 8:-65725510 None:intergenic
AATTCTGGTTTGATGATGAA+GGG 0.584821 8:+65725996 MsG0880045870.01.T01:CDS
GTTCACACGTTGGAACAAGT+AGG 0.594798 8:+65725888 MsG0880045870.01.T01:CDS
TCACAATCACACATTGGTGG+TGG 0.595725 8:+65725924 MsG0880045870.01.T01:CDS
GAAGAAACAAGTTCACACGT+TGG 0.614768 8:+65725878 MsG0880045870.01.T01:CDS
AGAATTCGATGGCAGCGATG+AGG 0.615506 8:+65725622 MsG0880045870.01.T01:CDS
TGTAGCTGCTAGGCATAGAA+GGG 0.616015 8:+65725749 MsG0880045870.01.T01:CDS
CTGCTAGGCATAGAAGGGAA+AGG 0.619057 8:+65725754 MsG0880045870.01.T01:CDS
ATCCACAAAGTGTAGCTGCT+AGG 0.619623 8:+65725739 MsG0880045870.01.T01:CDS
AAGGAATGTGAAGATATCAA+AGG 0.620019 8:+65725716 MsG0880045870.01.T01:CDS
GAGTTGGTTTGAAGAGATGG+TGG 0.649591 8:-65725558 None:intergenic
AATAGATCACAATCACACAT+TGG 0.653131 8:+65725918 MsG0880045870.01.T01:CDS
AGATCACAATCACACATTGG+TGG 0.659957 8:+65725921 MsG0880045870.01.T01:CDS
TCACACGTTGGAACAAGTAG+GGG 0.679972 8:+65725890 MsG0880045870.01.T01:CDS
ATATTACAAAGATTAGTACC+TGG 0.683168 8:+65725795 MsG0880045870.01.T01:CDS
TTACAAAGATTAGTACCTGG+TGG 0.689802 8:+65725798 MsG0880045870.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATGTGAATTATAATTC+TGG + Chr8:65725981-65726000 MsG0880045870.01.T01:CDS 15.0%
!! TCAATATTATTCAAACTTTG+TGG - Chr8:65725489-65725508 None:intergenic 20.0%
!!! TTTTGGTTAAGGTTAAATAA+TGG - Chr8:65725453-65725472 None:intergenic 20.0%
! ATATTACAAAGATTAGTACC+TGG + Chr8:65725795-65725814 MsG0880045870.01.T01:CDS 25.0%
!!! TTTTGAAAAGAAGAATTCGA+TGG + Chr8:65725611-65725630 MsG0880045870.01.T01:CDS 25.0%
AAGGAATGTGAAGATATCAA+AGG + Chr8:65725716-65725735 MsG0880045870.01.T01:CDS 30.0%
AATAGATCACAATCACACAT+TGG + Chr8:65725918-65725937 MsG0880045870.01.T01:CDS 30.0%
! AAATAATGGAACAGTGTTGT+TGG - Chr8:65725439-65725458 None:intergenic 30.0%
!! AATTCTGGTTTGATGATGAA+GGG + Chr8:65725996-65726015 MsG0880045870.01.T01:CDS 30.0%
!! GTGTGATATTCAAGTTTTGT+TGG - Chr8:65725539-65725558 None:intergenic 30.0%
!! GTTTTGTTGGAAATTGACAT+TGG - Chr8:65725526-65725545 None:intergenic 30.0%
!! TAATTCTGGTTTGATGATGA+AGG + Chr8:65725995-65726014 MsG0880045870.01.T01:CDS 30.0%
!!! ATGATAGTGCATTTTGGTTA+AGG - Chr8:65725464-65725483 None:intergenic 30.0%
AAAGGATAAGTGAGAGGATA+AGG + Chr8:65725772-65725791 MsG0880045870.01.T01:CDS 35.0%
ATCTTCACATTCCTTCTCTT+CGG - Chr8:65725711-65725730 None:intergenic 35.0%
GTAAGGAAATGATGAAGAGT+TGG - Chr8:65725577-65725596 None:intergenic 35.0%
TTACAAAGATTAGTACCTGG+TGG + Chr8:65725798-65725817 MsG0880045870.01.T01:CDS 35.0%
! AAAGGTTGATCAGAGAATTC+AGG - Chr8:65725384-65725403 None:intergenic 35.0%
! AAGGTTGATCAGAGAATTCA+GGG - Chr8:65725383-65725402 None:intergenic 35.0%
! GATAGCAATGATGATGCAAA+TGG + Chr8:65725350-65725369 MsG0880045870.01.T01:CDS 35.0%
! TGTCAACCTTTTCAAATGGT+GGG + Chr8:65726020-65726039 MsG0880045870.01.T01:CDS 35.0%
! TTGTCAACCTTTTCAAATGG+TGG + Chr8:65726019-65726038 MsG0880045870.01.T01:CDS 35.0%
AAAGAATTCGACTGCGAGTA+AGG - Chr8:65725594-65725613 None:intergenic 40.0%
AGATCACAATCACACATTGG+TGG + Chr8:65725921-65725940 MsG0880045870.01.T01:CDS 40.0%
GAAGAAACAAGTTCACACGT+TGG + Chr8:65725878-65725897 MsG0880045870.01.T01:CDS 40.0%
GATTCGGGATCGATATGTAT+AGG - Chr8:65725678-65725697 None:intergenic 40.0%
GCTAAGCAATTGTTTGGAAG+TGG - Chr8:65726048-65726067 None:intergenic 40.0%
GTGATTTCTTGGTAAGTGGT+GGG - Chr8:65725417-65725436 None:intergenic 40.0%
TTCACACGTTGGAACAAGTA+GGG + Chr8:65725889-65725908 MsG0880045870.01.T01:CDS 40.0%
TTGACATTGGAGTGTAGAAG+TGG - Chr8:65725513-65725532 None:intergenic 40.0%
! CATTGAAGCAGTGTCCATTT+TGG - Chr8:65725826-65725845 None:intergenic 40.0%
! GGGTTGTCAACCTTTTCAAA+TGG + Chr8:65726016-65726035 MsG0880045870.01.T01:CDS 40.0%
!! CAGTGTTGTTGGTGATTTCT+TGG - Chr8:65725428-65725447 None:intergenic 40.0%
!! GAAGAGTTGGTTTGAAGAGA+TGG - Chr8:65725564-65725583 None:intergenic 40.0%
!! GTGGAGATGATAGTGCATTT+TGG - Chr8:65725470-65725489 None:intergenic 40.0%
!! GTGGTGGGAGTGTTATAAAA+AGG - Chr8:65725402-65725421 None:intergenic 40.0%
!! GTTGGTGATTTCTTGGTAAG+TGG - Chr8:65725421-65725440 None:intergenic 40.0%
AGTACCTGGTGGAACCAAAA+TGG + Chr8:65725809-65725828 MsG0880045870.01.T01:CDS 45.0%
ATATCGATCCCGAATCCGTT+AGG + Chr8:65725682-65725701 MsG0880045870.01.T01:CDS 45.0%
ATCCACAAAGTGTAGCTGCT+AGG + Chr8:65725739-65725758 MsG0880045870.01.T01:CDS 45.0%
GAAGGGAAAGGATAAGTGAG+AGG + Chr8:65725766-65725785 MsG0880045870.01.T01:CDS 45.0%
GGTGATTTCTTGGTAAGTGG+TGG - Chr8:65725418-65725437 None:intergenic 45.0%
GTGGAACCCACCATTTGAAA+AGG - Chr8:65726029-65726048 None:intergenic 45.0%
GTTCACACGTTGGAACAAGT+AGG + Chr8:65725888-65725907 MsG0880045870.01.T01:CDS 45.0%
TCACAATCACACATTGGTGG+TGG + Chr8:65725924-65725943 MsG0880045870.01.T01:CDS 45.0%
TCACACGTTGGAACAAGTAG+GGG + Chr8:65725890-65725909 MsG0880045870.01.T01:CDS 45.0%
TGTAGCTGCTAGGCATAGAA+GGG + Chr8:65725749-65725768 MsG0880045870.01.T01:CDS 45.0%
TTCACATTCCTTCTCTTCGG+CGG - Chr8:65725708-65725727 None:intergenic 45.0%
!! ATTGGTGGTGGATTCATTGG+AGG + Chr8:65725936-65725955 MsG0880045870.01.T01:CDS 45.0%
!! CACATTGGTGGTGGATTCAT+TGG + Chr8:65725933-65725952 MsG0880045870.01.T01:CDS 45.0%
!! GAGTTGGTTTGAAGAGATGG+TGG - Chr8:65725561-65725580 None:intergenic 45.0%
!! TTGGTGGTGGATTCATTGGA+GGG + Chr8:65725937-65725956 MsG0880045870.01.T01:CDS 45.0%
AGAATTCGATGGCAGCGATG+AGG + Chr8:65725622-65725641 MsG0880045870.01.T01:CDS 50.0%
CTGCTAGGCATAGAAGGGAA+AGG + Chr8:65725754-65725773 MsG0880045870.01.T01:CDS 50.0%
GAATTCGATGGCAGCGATGA+GGG + Chr8:65725623-65725642 MsG0880045870.01.T01:CDS 50.0%
GTGTAGCTGCTAGGCATAGA+AGG + Chr8:65725748-65725767 MsG0880045870.01.T01:CDS 50.0%
TGCCTAGCAGCTACACTTTG+TGG - Chr8:65725744-65725763 None:intergenic 50.0%
!!! GTGTCCATTTTGGTTCCACC+AGG - Chr8:65725816-65725835 None:intergenic 50.0%
CCTTCTCTTCGGCGGCCTAA+CGG - Chr8:65725700-65725719 None:intergenic 60.0%
!! CTTCGGCGGCCTAACGGATT+CGG - Chr8:65725694-65725713 None:intergenic 60.0%
!! TTCGGCGGCCTAACGGATTC+GGG - Chr8:65725693-65725712 None:intergenic 60.0%
CCGTTAGGCCGCCGAAGAGA+AGG + Chr8:65725697-65725716 MsG0880045870.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 65725342 65726070 65725342 ID=MsG0880045870.01;Name=MsG0880045870.01
Chr8 mRNA 65725342 65726070 65725342 ID=MsG0880045870.01.T01;Parent=MsG0880045870.01;Name=MsG0880045870.01.T01;_AED=0.21;_eAED=0.21;_QI=0|-1|0|1|-1|1|1|0|242
Chr8 exon 65725342 65726070 65725342 ID=MsG0880045870.01.T01:exon:13640;Parent=MsG0880045870.01.T01
Chr8 CDS 65725342 65726070 65725342 ID=MsG0880045870.01.T01:cds;Parent=MsG0880045870.01.T01
Gene Sequence

>MsG0880045870.01.T01

ATGGACATGATAGCAATGATGATGCAAATGGAAAAGTTCCCTGAATTCTCTGATCAACCTTTTTATAACACTCCCACCACTTACCAAGAAATCACCAACAACACTGTTCCATTATTTAACCTTAACCAAAATGCACTATCATCTCCACAAAGTTTGAATAATATTGATCCACTTCTACACTCCAATGTCAATTTCCAACAAAACTTGAATATCACACCACCATCTCTTCAAACCAACTCTTCATCATTTCCTTACTCGCAGTCGAATTCTTTTGAAAAGAAGAATTCGATGGCAGCGATGAGGGAAATGATATTTAGAATAGCAGTGATGCAACCTATACATATCGATCCCGAATCCGTTAGGCCGCCGAAGAGAAGGAATGTGAAGATATCAAAGGATCCACAAAGTGTAGCTGCTAGGCATAGAAGGGAAAGGATAAGTGAGAGGATAAGGATATTACAAAGATTAGTACCTGGTGGAACCAAAATGGACACTGCTTCAATGTTAGATGAAGCTATACATTATGTGAAGTTTTTGAAGAAACAAGTTCACACGTTGGAACAAGTAGGGGCTAATAATAGATCACAATCACACATTGGTGGTGGATTCATTGGAGGGAATAATATGATGAACAATTTTAATAATGTGAATTATAATTCTGGTTTGATGATGAAGGGTTGTCAACCTTTTCAAATGGTGGGTTCCACTTCCAAACAATTGCTTAGCTAG

Protein sequence

>MsG0880045870.01.T01

MDMIAMMMQMEKFPEFSDQPFYNTPTTYQEITNNTVPLFNLNQNALSSPQSLNNIDPLLHSNVNFQQNLNITPPSLQTNSSSFPYSQSNSFEKKNSMAAMREMIFRIAVMQPIHIDPESVRPPKRRNVKISKDPQSVAARHRRERISERIRILQRLVPGGTKMDTASMLDEAIHYVKFLKKQVHTLEQVGANNRSQSHIGGGFIGGNNMMNNFNNVNYNSGLMMKGCQPFQMVGSTSKQLLS*