Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046459.01.T01 | RHN63666.1 | 83.854 | 192 | 23 | 1 | 17 | 200 | 82 | 273 | 5.76E-107 | 318 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046459.01.T01 | Q9M9U9 | 65.217 | 115 | 39 | 1 | 79 | 192 | 125 | 239 | 1.96E-47 | 159 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046459.01.T01 | A0A396IDK0 | 83.854 | 192 | 23 | 1 | 17 | 200 | 82 | 273 | 2.75e-107 | 318 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0880046459.01.T01 | TF | RWP-RK |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046459.01.T01 | MTR_0140s0010 | 70.690 | 174 | 29 | 2 | 17 | 182 | 61 | 220 | 4.52e-76 | 228 |
| MsG0880046459.01.T01 | MTR_3g435550 | 54.762 | 168 | 63 | 4 | 36 | 194 | 5 | 168 | 9.60e-54 | 170 |
| MsG0880046459.01.T01 | MTR_3g091700 | 50.862 | 116 | 48 | 2 | 79 | 194 | 151 | 257 | 1.01e-30 | 113 |
| MsG0880046459.01.T01 | MTR_5g024070 | 35.185 | 162 | 90 | 3 | 39 | 196 | 169 | 319 | 1.61e-23 | 95.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046459.01.T01 | AT1G18790 | 65.217 | 115 | 39 | 1 | 79 | 192 | 125 | 239 | 1.99e-48 | 159 |
| MsG0880046459.01.T01 | AT1G74480 | 65.138 | 109 | 37 | 1 | 81 | 188 | 134 | 242 | 3.54e-45 | 152 |
| MsG0880046459.01.T01 | AT5G66990 | 58.678 | 121 | 48 | 2 | 76 | 194 | 153 | 273 | 1.35e-42 | 144 |
| MsG0880046459.01.T01 | AT5G53040 | 57.547 | 106 | 36 | 1 | 87 | 192 | 153 | 249 | 1.22e-34 | 123 |
| MsG0880046459.01.T01 | AT4G35590 | 34.667 | 150 | 89 | 3 | 46 | 189 | 207 | 353 | 2.38e-19 | 85.1 |
| MsG0880046459.01.T01 | AT4G35590 | 34.667 | 150 | 89 | 3 | 46 | 189 | 157 | 303 | 2.52e-19 | 84.3 |
| MsG0880046459.01.T01 | AT5G16100 | 41.176 | 85 | 48 | 1 | 79 | 161 | 351 | 435 | 1.20e-13 | 68.9 |
Find 36 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTCTTGCAGCTTGTGTTAT+TGG | 0.135392 | 8:-73628669 | None:intergenic |
| GTTGATGGATATCGAGAAAT+TGG | 0.245834 | 8:+73628962 | MsG0880046459.01.T01:CDS |
| TGATCTTGCAATTGTATATC+TGG | 0.302877 | 8:-73629014 | None:intergenic |
| AACCTTGATCAATAATATTC+AGG | 0.325902 | 8:+73628779 | MsG0880046459.01.T01:CDS |
| ACAATGTTGTCTAAGCCTTT+TGG | 0.326924 | 8:-73629039 | None:intergenic |
| TTACAAGAAAAGAAAGCTTA+TGG | 0.338403 | 8:+73629073 | MsG0880046459.01.T01:CDS |
| CTGCAGACGACTATGAAATT+TGG | 0.367816 | 8:+73628457 | MsG0880046459.01.T01:CDS |
| ACTTAGTGTTGAAAAGTTGA+TGG | 0.379342 | 8:+73628947 | MsG0880046459.01.T01:CDS |
| TATTATTGATCAAGGTTTGC+AGG | 0.383508 | 8:-73628773 | None:intergenic |
| TACCTGAATATTATTGATCA+AGG | 0.420609 | 8:-73628781 | None:intergenic |
| TATGGTATGGAGAAAGGATT+TGG | 0.421835 | 8:+73628480 | MsG0880046459.01.T01:CDS |
| TGAAATTAACAAAGAGGTTG+TGG | 0.431311 | 8:+73628560 | MsG0880046459.01.T01:CDS |
| ATTTGGTATGGTATGGAGAA+AGG | 0.442668 | 8:+73628474 | MsG0880046459.01.T01:CDS |
| TGTTCTTCTGATTAATATTG+TGG | 0.475779 | 8:+73628434 | MsG0880046459.01.T01:CDS |
| CTATGAAATTTGGTATGGTA+TGG | 0.481213 | 8:+73628467 | MsG0880046459.01.T01:CDS |
| AGAGGAAATGAAGCTGGTGG+AGG | 0.485504 | 8:+73628986 | MsG0880046459.01.T01:CDS |
| GTTGCAGGAGCTAGGGAAAG+AGG | 0.496134 | 8:+73628917 | MsG0880046459.01.T01:intron |
| TGGATTTGGTTGCAGGAGCT+AGG | 0.503102 | 8:+73628909 | MsG0880046459.01.T01:intron |
| GGAGTGGAGAGGATTAGTGA+TGG | 0.512454 | 8:+73628597 | MsG0880046459.01.T01:CDS |
| GGATTTGGTTGCAGGAGCTA+GGG | 0.512821 | 8:+73628910 | MsG0880046459.01.T01:intron |
| GGAGGTAAAGCTAGAAGGAG+TGG | 0.514082 | 8:+73628581 | MsG0880046459.01.T01:CDS |
| GTTGTGGAGGTAAAGCTAGA+AGG | 0.522008 | 8:+73628576 | MsG0880046459.01.T01:CDS |
| GGATATCGAGAAATTGGAAG+AGG | 0.537710 | 8:+73628968 | MsG0880046459.01.T01:CDS |
| GACGACTATGAAATTTGGTA+TGG | 0.546215 | 8:+73628462 | MsG0880046459.01.T01:CDS |
| ATTGGAAGAGGAAATGAAGC+TGG | 0.556172 | 8:+73628980 | MsG0880046459.01.T01:CDS |
| AATTGCAAGATCATACCAAA+AGG | 0.561321 | 8:+73629024 | MsG0880046459.01.T01:CDS |
| TAAAGCTAGAAGGAGTGGAG+AGG | 0.578999 | 8:+73628586 | MsG0880046459.01.T01:CDS |
| TGGAAATGAAATTAACAAAG+AGG | 0.597270 | 8:+73628554 | MsG0880046459.01.T01:CDS |
| AGCTGCAAGAGAACTGAATG+TGG | 0.601755 | 8:+73628680 | MsG0880046459.01.T01:CDS |
| GTAGGGAGTTGGGTATTCGT+AGG | 0.615855 | 8:+73628727 | MsG0880046459.01.T01:CDS |
| AATTAACAAAGAGGTTGTGG+AGG | 0.641037 | 8:+73628563 | MsG0880046459.01.T01:CDS |
| GCTGCAAGAGAACTGAATGT+GGG | 0.643443 | 8:+73628681 | MsG0880046459.01.T01:CDS |
| GGGAGTTGGGTATTCGTAGG+TGG | 0.654663 | 8:+73628730 | MsG0880046459.01.T01:CDS |
| GTAACACAAAGAATTTGTCA+AGG | 0.655690 | 8:+73628619 | MsG0880046459.01.T01:CDS |
| GGAAGAGGAAATGAAGCTGG+TGG | 0.660598 | 8:+73628983 | MsG0880046459.01.T01:CDS |
| CAGGAGCTAGGGAAAGAGGA+TGG | 0.660990 | 8:+73628921 | MsG0880046459.01.T01:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CATATAAAAATACTGAGATA+TGG | - | Chr8:73628649-73628668 | None:intergenic | 20.0% |
| !!! | AACATTATGTTGATTGTTTA+TGG | + | Chr8:73628872-73628891 | MsG0880046459.01.T01:intron | 20.0% |
| ! | AACCTTGATCAATAATATTC+AGG | + | Chr8:73628779-73628798 | MsG0880046459.01.T01:CDS | 25.0% |
| ! | TACCTGAATATTATTGATCA+AGG | - | Chr8:73628784-73628803 | None:intergenic | 25.0% |
| ! | TGAGTATATTATGTTGGATT+TGG | + | Chr8:73628895-73628914 | MsG0880046459.01.T01:intron | 25.0% |
| ! | TGGAAATGAAATTAACAAAG+AGG | + | Chr8:73628554-73628573 | MsG0880046459.01.T01:CDS | 25.0% |
| ! | TGTTCTTCTGATTAATATTG+TGG | + | Chr8:73628434-73628453 | MsG0880046459.01.T01:CDS | 25.0% |
| ! | TTACAAGAAAAGAAAGCTTA+TGG | + | Chr8:73629073-73629092 | MsG0880046459.01.T01:CDS | 25.0% |
| ! | TTATGGTGAGTATATTATGT+TGG | + | Chr8:73628889-73628908 | MsG0880046459.01.T01:intron | 25.0% |
| !! | AAGCTTTCTTTTCTTGTAAT+TGG | - | Chr8:73629072-73629091 | None:intergenic | 25.0% |
| AATTGCAAGATCATACCAAA+AGG | + | Chr8:73629024-73629043 | MsG0880046459.01.T01:CDS | 30.0% | |
| ACATTTGAAAAAACGGTGTA+GGG | + | Chr8:73628710-73628729 | MsG0880046459.01.T01:CDS | 30.0% | |
| CTATGAAATTTGGTATGGTA+TGG | + | Chr8:73628467-73628486 | MsG0880046459.01.T01:CDS | 30.0% | |
| GTAACACAAAGAATTTGTCA+AGG | + | Chr8:73628619-73628638 | MsG0880046459.01.T01:CDS | 30.0% | |
| TATTATTGATCAAGGTTTGC+AGG | - | Chr8:73628776-73628795 | None:intergenic | 30.0% | |
| TGAAATTAACAAAGAGGTTG+TGG | + | Chr8:73628560-73628579 | MsG0880046459.01.T01:CDS | 30.0% | |
| TGATCTTGCAATTGTATATC+TGG | - | Chr8:73629017-73629036 | None:intergenic | 30.0% | |
| ! | ACTTAGTGTTGAAAAGTTGA+TGG | + | Chr8:73628947-73628966 | MsG0880046459.01.T01:CDS | 30.0% |
| !! | GCTTAGACAACATTGTTTTA+AGG | + | Chr8:73629046-73629065 | MsG0880046459.01.T01:CDS | 30.0% |
| AATTAACAAAGAGGTTGTGG+AGG | + | Chr8:73628563-73628582 | MsG0880046459.01.T01:CDS | 35.0% | |
| ATTTGGTATGGTATGGAGAA+AGG | + | Chr8:73628474-73628493 | MsG0880046459.01.T01:CDS | 35.0% | |
| CACATTTGAAAAAACGGTGT+AGG | + | Chr8:73628709-73628728 | MsG0880046459.01.T01:CDS | 35.0% | |
| GACGACTATGAAATTTGGTA+TGG | + | Chr8:73628462-73628481 | MsG0880046459.01.T01:CDS | 35.0% | |
| GCCTCACACATTTGAAAAAA+CGG | + | Chr8:73628703-73628722 | MsG0880046459.01.T01:CDS | 35.0% | |
| TATGGTATGGAGAAAGGATT+TGG | + | Chr8:73628480-73628499 | MsG0880046459.01.T01:CDS | 35.0% | |
| ! | ACAATGTTGTCTAAGCCTTT+TGG | - | Chr8:73629042-73629061 | None:intergenic | 35.0% |
| ! | ATTATGTTGGATTTGGTTGC+AGG | + | Chr8:73628902-73628921 | MsG0880046459.01.T01:intron | 35.0% |
| ! | GCGTTTGATTCTGAAAAAAG+TGG | + | Chr8:73628534-73628553 | MsG0880046459.01.T01:CDS | 35.0% |
| ! | GTTGATGGATATCGAGAAAT+TGG | + | Chr8:73628962-73628981 | MsG0880046459.01.T01:CDS | 35.0% |
| !!! | ACCGTTTTTTCAAATGTGTG+AGG | - | Chr8:73628707-73628726 | None:intergenic | 35.0% |
| AAAAAACGGTGTAGGGAGTT+GGG | + | Chr8:73628717-73628736 | MsG0880046459.01.T01:CDS | 40.0% | |
| ATTGGAAGAGGAAATGAAGC+TGG | + | Chr8:73628980-73628999 | MsG0880046459.01.T01:CDS | 40.0% | |
| CTGCAGACGACTATGAAATT+TGG | + | Chr8:73628457-73628476 | MsG0880046459.01.T01:CDS | 40.0% | |
| GGATATCGAGAAATTGGAAG+AGG | + | Chr8:73628968-73628987 | MsG0880046459.01.T01:CDS | 40.0% | |
| TCTCTTGCAGCTTGTGTTAT+TGG | - | Chr8:73628672-73628691 | None:intergenic | 40.0% | |
| ! | AGGCTCATGAGTTTTCTATG+GGG | - | Chr8:73628756-73628775 | None:intergenic | 40.0% |
| ! | CAGGCTCATGAGTTTTCTAT+GGG | - | Chr8:73628757-73628776 | None:intergenic | 40.0% |
| AGCTGCAAGAGAACTGAATG+TGG | + | Chr8:73628680-73628699 | MsG0880046459.01.T01:CDS | 45.0% | |
| GAAAAAACGGTGTAGGGAGT+TGG | + | Chr8:73628716-73628735 | MsG0880046459.01.T01:CDS | 45.0% | |
| GCTGCAAGAGAACTGAATGT+GGG | + | Chr8:73628681-73628700 | MsG0880046459.01.T01:CDS | 45.0% | |
| GTTGTGGAGGTAAAGCTAGA+AGG | + | Chr8:73628576-73628595 | MsG0880046459.01.T01:CDS | 45.0% | |
| TAAAGCTAGAAGGAGTGGAG+AGG | + | Chr8:73628586-73628605 | MsG0880046459.01.T01:CDS | 45.0% | |
| ! | GCAGGCTCATGAGTTTTCTA+TGG | - | Chr8:73628758-73628777 | None:intergenic | 45.0% |
| !!! | GTTATTATTATTATTATTTT+TGG | - | Chr8:73628855-73628874 | None:intergenic | 5.0% |
| AGAGGAAATGAAGCTGGTGG+AGG | + | Chr8:73628986-73629005 | MsG0880046459.01.T01:CDS | 50.0% | |
| GGAAGAGGAAATGAAGCTGG+TGG | + | Chr8:73628983-73629002 | MsG0880046459.01.T01:CDS | 50.0% | |
| GGAGGTAAAGCTAGAAGGAG+TGG | + | Chr8:73628581-73628600 | MsG0880046459.01.T01:CDS | 50.0% | |
| GTAGGGAGTTGGGTATTCGT+AGG | + | Chr8:73628727-73628746 | MsG0880046459.01.T01:CDS | 50.0% | |
| ! | GGAGTGGAGAGGATTAGTGA+TGG | + | Chr8:73628597-73628616 | MsG0880046459.01.T01:CDS | 50.0% |
| ! | GGATTTGGTTGCAGGAGCTA+GGG | + | Chr8:73628910-73628929 | MsG0880046459.01.T01:intron | 50.0% |
| ! | TGGATTTGGTTGCAGGAGCT+AGG | + | Chr8:73628909-73628928 | MsG0880046459.01.T01:intron | 50.0% |
| CAGGAGCTAGGGAAAGAGGA+TGG | + | Chr8:73628921-73628940 | MsG0880046459.01.T01:intron | 55.0% | |
| GGGAGTTGGGTATTCGTAGG+TGG | + | Chr8:73628730-73628749 | MsG0880046459.01.T01:CDS | 55.0% | |
| GTTGCAGGAGCTAGGGAAAG+AGG | + | Chr8:73628917-73628936 | MsG0880046459.01.T01:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 73628390 | 73629115 | 73628390 | ID=MsG0880046459.01;Name=MsG0880046459.01 |
| Chr8 | mRNA | 73628390 | 73629115 | 73628390 | ID=MsG0880046459.01.T01;Parent=MsG0880046459.01;Name=MsG0880046459.01.T01;_AED=0.48;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|200 |
| Chr8 | exon | 73628390 | 73628800 | 73628390 | ID=MsG0880046459.01.T01:exon:7947;Parent=MsG0880046459.01.T01 |
| Chr8 | exon | 73628924 | 73629115 | 73628924 | ID=MsG0880046459.01.T01:exon:7948;Parent=MsG0880046459.01.T01 |
| Chr8 | CDS | 73628390 | 73628800 | 73628390 | ID=MsG0880046459.01.T01:cds;Parent=MsG0880046459.01.T01 |
| Chr8 | CDS | 73628924 | 73629115 | 73628924 | ID=MsG0880046459.01.T01:cds;Parent=MsG0880046459.01.T01 |
| Gene Sequence |
| Protein sequence |
