AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046546.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046546.01.T01 MTR_4g109770 96.383 470 17 0 1 470 1 470 0.0 938
MsG0880046546.01.T01 MTR_2g461920 61.866 493 149 11 12 470 52 539 1.86e-171 493
MsG0880046546.01.T01 MTR_5g046670 49.315 219 88 8 56 264 174 379 1.18e-50 179
MsG0880046546.01.T01 MTR_3g099080 54.348 138 42 4 75 198 43 173 3.89e-38 143
MsG0880046546.01.T01 MTR_7g028740 35.331 317 179 8 75 382 26 325 8.79e-38 142
MsG0880046546.01.T01 MTR_8g096780 38.194 288 132 11 75 339 45 309 1.12e-36 139
MsG0880046546.01.T01 MTR_7g444860 70.000 80 24 0 117 196 143 222 6.38e-34 130
MsG0880046546.01.T01 MTR_7g092930 69.136 81 25 0 117 197 62 142 7.03e-34 130
MsG0880046546.01.T01 MTR_3g085180 50.769 130 57 2 72 198 115 240 8.36e-34 132
MsG0880046546.01.T01 MTR_1g053715 38.197 233 106 6 123 351 74 272 1.71e-33 129
MsG0880046546.01.T01 MTR_8g005960 73.684 76 20 0 123 198 187 262 4.90e-33 128
MsG0880046546.01.T01 MTR_4g088555 72.973 74 20 0 123 196 63 136 7.57e-33 122
MsG0880046546.01.T01 MTR_2g014200 70.886 79 23 0 118 196 59 137 3.22e-32 120
MsG0880046546.01.T01 MTR_8g463140 70.270 74 22 0 123 196 65 138 2.84e-31 119
MsG0880046546.01.T01 MTR_2g078770 70.270 74 22 0 123 196 58 131 1.19e-30 117
MsG0880046546.01.T01 MTR_7g110320 69.737 76 23 0 121 196 143 218 1.56e-30 125
MsG0880046546.01.T01 MTR_2g046550 45.946 148 69 3 58 196 67 212 2.36e-30 125
MsG0880046546.01.T01 MTR_7g110320 69.737 76 23 0 121 196 143 218 2.53e-30 125
MsG0880046546.01.T01 MTR_1g035010 62.366 93 31 1 110 198 87 179 1.56e-29 123
MsG0880046546.01.T01 MTR_1g086250 69.737 76 23 0 121 196 145 220 1.94e-29 122
MsG0880046546.01.T01 MTR_8g080680 36.607 224 103 8 122 339 131 321 2.59e-28 116
MsG0880046546.01.T01 MTR_8g080690 63.158 76 28 0 123 198 151 226 5.34e-28 115
MsG0880046546.01.T01 MTR_8g080670 61.644 73 28 0 123 195 162 234 4.13e-25 107
MsG0880046546.01.T01 MTR_2g020620 51.562 64 31 0 133 196 100 163 2.30e-16 82.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046546.01.T01 AT1G69170 64.286 126 32 3 75 197 83 198 7.80e-45 162
MsG0880046546.01.T01 AT5G50670 46.067 178 84 4 123 288 101 278 2.57e-37 140
MsG0880046546.01.T01 AT5G50670 46.067 178 84 4 123 288 101 278 2.57e-37 140
MsG0880046546.01.T01 AT5G50570 46.067 178 84 4 123 288 101 278 2.57e-37 140
MsG0880046546.01.T01 AT5G50570 46.067 178 84 4 123 288 101 278 2.57e-37 140
MsG0880046546.01.T01 AT1G27370 68.966 87 27 0 123 209 176 262 2.36e-36 137
MsG0880046546.01.T01 AT1G27370 68.966 87 27 0 123 209 176 262 5.57e-36 137
MsG0880046546.01.T01 AT1G27370 68.966 87 27 0 123 209 176 262 5.57e-36 137
MsG0880046546.01.T01 AT1G27370 68.966 87 27 0 123 209 176 262 5.57e-36 137
MsG0880046546.01.T01 AT1G27370 68.966 87 27 0 123 209 176 262 5.57e-36 137
MsG0880046546.01.T01 AT1G27370 68.966 87 27 0 123 209 176 262 5.57e-36 137
MsG0880046546.01.T01 AT1G27370 68.966 87 27 0 123 209 176 262 5.57e-36 137
MsG0880046546.01.T01 AT1G27360 46.429 168 65 4 53 209 108 261 4.57e-35 135
MsG0880046546.01.T01 AT1G27360 46.429 168 65 4 53 209 108 261 4.57e-35 135
MsG0880046546.01.T01 AT1G27360 46.429 168 65 4 53 209 108 261 4.57e-35 135
MsG0880046546.01.T01 AT1G27360 46.429 168 65 4 53 209 108 261 4.57e-35 135
MsG0880046546.01.T01 AT5G43270 46.199 171 68 6 48 209 94 249 6.89e-34 132
MsG0880046546.01.T01 AT5G43270 46.199 171 68 6 48 209 94 249 6.89e-34 132
MsG0880046546.01.T01 AT5G43270 46.199 171 68 6 48 209 94 249 6.89e-34 132
MsG0880046546.01.T01 AT2G33810 67.089 79 26 0 118 196 49 127 6.71e-33 121
MsG0880046546.01.T01 AT3G57920 70.588 85 25 0 114 198 50 134 1.35e-32 127
MsG0880046546.01.T01 AT2G42200 75.000 76 19 0 123 198 74 149 1.77e-32 127
MsG0880046546.01.T01 AT1G02065 72.727 77 21 0 120 196 185 261 2.14e-32 126
MsG0880046546.01.T01 AT2G47070 73.684 76 20 0 121 196 104 179 2.61e-31 128
MsG0880046546.01.T01 AT3G60030 72.973 74 20 0 123 196 127 200 4.32e-31 127
MsG0880046546.01.T01 AT1G53160 51.667 120 48 3 123 232 54 173 4.83e-31 118
MsG0880046546.01.T01 AT1G53160 51.667 120 48 3 123 232 54 173 4.83e-31 118
MsG0880046546.01.T01 AT1G20980 62.000 100 35 1 110 206 104 203 8.55e-31 127
MsG0880046546.01.T01 AT1G76580 72.368 76 21 0 123 198 114 189 3.32e-30 124
MsG0880046546.01.T01 AT1G76580 72.368 76 21 0 123 198 114 189 3.36e-30 124
MsG0880046546.01.T01 AT1G53160 62.069 87 31 1 123 207 54 140 4.33e-30 115
MsG0880046546.01.T01 AT1G76580 72.368 76 21 0 123 198 114 189 6.46e-30 124
MsG0880046546.01.T01 AT3G15270 63.636 77 28 0 123 199 63 139 1.04e-28 112
MsG0880046546.01.T01 AT5G18830 54.023 87 39 1 112 197 127 213 2.19e-22 101
MsG0880046546.01.T01 AT5G18830 54.023 87 39 1 112 197 127 213 2.27e-22 101
MsG0880046546.01.T01 AT5G18830 54.023 87 39 1 112 197 127 213 2.41e-22 100
MsG0880046546.01.T01 AT2G47070 76.000 50 12 0 147 196 1 50 5.19e-18 87.4
MsG0880046546.01.T01 AT1G02065 64.706 51 18 0 120 170 185 235 2.51e-16 79.0

Find 96 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTGCATATTGTCAAGTTTA+TGG 0.148069 8:-74654945 MsG0880046546.01.T01:CDS
AGGTCTTCCAAAGTGGTTTC+TGG 0.202370 8:-74653432 MsG0880046546.01.T01:CDS
GGCGTTCACTCTACAAATTC+TGG 0.217076 8:-74654010 MsG0880046546.01.T01:CDS
CTGCTGGTGGCTTGGTTCCC+TGG 0.251209 8:+74653473 None:intergenic
GGACTGGCTTGAAGTGTTAT+TGG 0.259748 8:+74653102 None:intergenic
CGAAATCACTTTAAGTAATT+CGG 0.269479 8:+74652723 None:intergenic
AGTTATATTTCTGAAGAAAA+AGG 0.280021 8:-74655290 MsG0880046546.01.T01:CDS
CACAAACTTTATGCCTCTTA+TGG 0.303462 8:+74654889 None:intergenic
CAAAATAGGTGCTGCTCCTT+TGG 0.306496 8:+74655019 None:intergenic
GGGAGACTGTTTCAGCCATT+TGG 0.327396 8:-74653989 MsG0880046546.01.T01:CDS
TATGGCCTGCAAGTCGTTTA+CGG 0.342741 8:+74654056 None:intergenic
TCAGATGATTTCAATTTCAT+TGG 0.348900 8:-74655224 MsG0880046546.01.T01:CDS
CCAAGCATTCAAGGATTATC+AGG 0.351371 8:-74653166 MsG0880046546.01.T01:CDS
GCTTCATGTTCTGCTTTCAT+AGG 0.354620 8:+74653406 None:intergenic
TCCATCTGTAGGATTTAATT+CGG 0.357577 8:+74652970 None:intergenic
TTATTGTCTGGTACCAATAT+AGG 0.364521 8:+74652935 None:intergenic
CGACAGTTGTAGTAAATTGA+TGG 0.372715 8:+74652820 None:intergenic
GTTCCAAGCAAGAAACTTGA+TGG 0.403736 8:-74655197 MsG0880046546.01.T01:CDS
TGAGTGCACTCTCACTCTCT+TGG 0.404810 8:+74654971 None:intergenic
CATACCGCACCAAGCATTCA+AGG 0.405157 8:-74653175 MsG0880046546.01.T01:CDS
CAAGGATCTTAGTTCTTGTA+AGG 0.406648 8:-74654916 MsG0880046546.01.T01:CDS
TGATATCGTGAACTTTGAAA+TGG 0.410198 8:-74652912 MsG0880046546.01.T01:CDS
GACTCACAAGAAGACAAAAT+AGG 0.410388 8:+74655005 None:intergenic
TCTTGCAGATATCAGATTCC+AGG 0.416127 8:-74653491 MsG0880046546.01.T01:intron
AATTCCCTTTGATAACCCTT+TGG 0.423487 8:-74653074 MsG0880046546.01.T01:CDS
CAGAACATGAAGCTGGCTTT+AGG 0.424799 8:-74653396 MsG0880046546.01.T01:CDS
TGTTGCATGCAATGGAATCT+TGG 0.426426 8:-74655313 None:intergenic
GAGTGCACTCTCACTCTCTT+GGG 0.430597 8:+74654972 None:intergenic
TTACCATCAAGTTTCTTGCT+TGG 0.431934 8:+74655194 None:intergenic
AGTTGCCGTAAACGACTTGC+AGG 0.435658 8:-74654061 MsG0880046546.01.T01:CDS
CCTGATAATCCTTGAATGCT+TGG 0.438418 8:+74653166 None:intergenic
AAGTCATCTCAGTCCTATAT+TGG 0.441537 8:-74652948 MsG0880046546.01.T01:CDS
ATTCCATTCACAATGACTAT+AGG 0.443748 8:+74654849 None:intergenic
CAAAGGGAATTTCCAAGGAC+TGG 0.449402 8:+74653086 None:intergenic
TCTCTCATTGATTTGAAGCT+AGG 0.463051 8:-74655086 MsG0880046546.01.T01:CDS
CACAATGACTATAGGAGTCT+TGG 0.465991 8:+74654857 None:intergenic
TAACACTTCAAGCCAGTCCT+TGG 0.468157 8:-74653098 MsG0880046546.01.T01:CDS
CATTCACAAAATCTGAATCA+TGG 0.469185 8:+74652876 None:intergenic
TCTTCTGTGCCTGTCACCAA+TGG 0.476821 8:-74653367 MsG0880046546.01.T01:CDS
AGCCAATGGCATGCGCATAA+TGG 0.479009 8:+74653233 None:intergenic
GCATCATAGACAATCATTGC+AGG 0.480777 8:-74652777 MsG0880046546.01.T01:CDS
GCGTTCACTCTACAAATTCT+GGG 0.481125 8:-74654009 MsG0880046546.01.T01:CDS
TTCACGATATCATTATTGTC+TGG 0.484373 8:+74652923 None:intergenic
AGCCATTATGCGCATGCCAT+TGG 0.493081 8:-74653235 MsG0880046546.01.T01:CDS
CTAGGAAGATTTGATGATCA+TGG 0.493709 8:-74655068 MsG0880046546.01.T01:CDS
GCCATTGGCTACACCTCCTT+AGG 0.499701 8:-74653220 MsG0880046546.01.T01:CDS
ATTGAAGTCTTCGCTTCCTA+AGG 0.501723 8:+74653204 None:intergenic
TATATATTATTACCACCAAA+TGG 0.502996 8:+74653974 None:intergenic
AAAAGATGCTGCTGGTGGCT+TGG 0.511710 8:+74653465 None:intergenic
TAGACATGGCTATGGCTGCT+TGG 0.512721 8:+74653046 None:intergenic
TGTGGTGGAATCCAATGGTT+GGG 0.513935 8:-74655111 MsG0880046546.01.T01:CDS
ACTCCTATAGTCATTGTGAA+TGG 0.516933 8:-74654852 MsG0880046546.01.T01:CDS
TTGCTGTCTGAGTTTGATGA+TGG 0.518570 8:-74654091 MsG0880046546.01.T01:CDS
TCCGAATTAAATCCTACAGA+TGG 0.523648 8:-74652971 MsG0880046546.01.T01:CDS
AATGAGAGAATCCCAACCAT+TGG 0.523865 8:+74655100 None:intergenic
AAACCACTTTGGAAGACCTC+TGG 0.529651 8:+74653436 None:intergenic
TAGGTCTCCAGAAACCACTT+TGG 0.532795 8:+74653425 None:intergenic
CTTGTAAGGACTACCATAAG+AGG 0.534199 8:-74654902 MsG0880046546.01.T01:CDS
TTTCAAGCTTTCAAATTCTG+TGG 0.537070 8:-74655129 MsG0880046546.01.T01:CDS
TTGCATCATCTTCACAAGAA+AGG 0.541164 8:+74652843 None:intergenic
TTATGCTGTAGACATGGCTA+TGG 0.541912 8:+74653038 None:intergenic
AATGTTGCCACATCCACTAC+AGG 0.543311 8:-74653280 MsG0880046546.01.T01:CDS
ATAACCAAAGGGTTATCAAA+GGG 0.544827 8:+74653070 None:intergenic
CAAGCTTTCAAATTCTGTGG+TGG 0.545205 8:-74655126 MsG0880046546.01.T01:CDS
GTTATCAAAGGGAATTTCCA+AGG 0.547080 8:+74653081 None:intergenic
AGACTGTTTCAGCCATTTGG+TGG 0.551343 8:-74653986 MsG0880046546.01.T01:intron
GGATAGAGAAAAGATGCTGC+TGG 0.557359 8:+74653457 None:intergenic
AATAACCAAAGGGTTATCAA+AGG 0.561243 8:+74653069 None:intergenic
TGGCTGCTTGGAATAACCAA+AGG 0.562021 8:+74653058 None:intergenic
CTGTGGTGGAATCCAATGGT+TGG 0.564108 8:-74655112 MsG0880046546.01.T01:CDS
ATGAAAGCAGAACATGAAGC+TGG 0.566946 8:-74653403 MsG0880046546.01.T01:CDS
GGCTGCTTGGAATAACCAAA+GGG 0.567623 8:+74653059 None:intergenic
GAAGTCTTCGCTTCCTAAGG+AGG 0.569996 8:+74653207 None:intergenic
GTGAACTTTGAAATGGAAGA+CGG 0.572384 8:-74652905 MsG0880046546.01.T01:CDS
CTTACTGCCTGTAGTGGATG+TGG 0.574967 8:+74653273 None:intergenic
TGAACTTTGAAATGGAAGAC+GGG 0.575100 8:-74652904 MsG0880046546.01.T01:CDS
ACTGAACTTACTGCCTGTAG+TGG 0.586429 8:+74653267 None:intergenic
CTGAGATGACTTCCATCTGT+AGG 0.587562 8:+74652959 None:intergenic
TCTTGATTGAAGATGTCCAT+TGG 0.588804 8:+74653351 None:intergenic
TCTATCAGACACTCGGCTTG+AGG 0.591247 8:+74653000 None:intergenic
TGAAGATGTCCATTGGTGAC+AGG 0.591626 8:+74653358 None:intergenic
CTTGCAGATATCAGATTCCA+GGG 0.591867 8:-74653490 MsG0880046546.01.T01:intron
TGCACTCTCACTCTCTTGGG+AGG 0.598164 8:+74654975 None:intergenic
TCCTAAGGAGGTGTAGCCAA+TGG 0.598734 8:+74653219 None:intergenic
TATCCAGAGGTCTTCCAAAG+TGG 0.601895 8:-74653439 MsG0880046546.01.T01:CDS
TTGTGAATGGAATTGAGCAG+AGG 0.621516 8:-74654839 MsG0880046546.01.T01:CDS
AATTCTGTGGTGGAATCCAA+TGG 0.627306 8:-74655116 MsG0880046546.01.T01:CDS
TGGCAATGCTTCAATTGATG+CGG 0.627501 8:-74655048 MsG0880046546.01.T01:CDS
TAAGAGGCATAAAGTTTGTG+AGG 0.628037 8:-74654886 MsG0880046546.01.T01:CDS
GTGATCTTATGCTGTAGACA+TGG 0.639068 8:+74653032 None:intergenic
ATAAAAGTCTATCAGACACT+CGG 0.644463 8:+74652993 None:intergenic
TCAAGTTTATGGATGTAACA+AGG 0.656151 8:-74654934 MsG0880046546.01.T01:CDS
TTTGTAGAGTGAACGCCTTG+TGG 0.665425 8:+74654016 None:intergenic
TAGAGAAAAGATGCTGCTGG+TGG 0.692709 8:+74653460 None:intergenic
TAATCCTTGAATGCTTGGTG+CGG 0.726498 8:+74653171 None:intergenic
GAGCGTCGAAGAAAACCACA+AGG 0.736819 8:-74654031 MsG0880046546.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGAATTAATTTAGTAAAA+TGG + Chr8:74653443-74653462 None:intergenic 10.0%
!! AAGAAATCAATGTAATTTAA+TGG + Chr8:74654163-74654182 None:intergenic 15.0%
!! AGAAATCAATGTAATTTAAT+GGG + Chr8:74654162-74654181 None:intergenic 15.0%
!! GGATACAATTATATAAAAAA+GGG + Chr8:74653395-74653414 None:intergenic 15.0%
!! TGGATACAATTATATAAAAA+AGG + Chr8:74653396-74653415 None:intergenic 15.0%
!!! TGTAAAATTGATTCTATATT+TGG - Chr8:74653284-74653303 MsG0880046546.01.T01:CDS 15.0%
!! ACTTATTCAACAATAGTTAA+TGG - Chr8:74654211-74654230 MsG0880046546.01.T01:intron 20.0%
!! ATTGTTGAATAAGTAACTTA+TGG + Chr8:74654205-74654224 None:intergenic 20.0%
!! GAAGTAATAAACTTGAAAAT+TGG + Chr8:74653248-74653267 None:intergenic 20.0%
!! TAGTAACTTAGTTATATGAA+TGG + Chr8:74654346-74654365 None:intergenic 20.0%
!! TATATATTATTACCACCAAA+TGG + Chr8:74654064-74654083 None:intergenic 20.0%
!!! AAAATTGATTCTATATTTGG+TGG - Chr8:74653287-74653306 MsG0880046546.01.T01:CDS 20.0%
!!! AAACTAATTTTGTTTAAGTC+TGG + Chr8:74653498-74653517 None:intergenic 20.0%
!!! AGTTATATTTCTGAAGAAAA+AGG - Chr8:74652745-74652764 MsG0880046546.01.T01:CDS 20.0%
! AAAACATGTGATCACAATTT+AGG + Chr8:74653716-74653735 None:intergenic 25.0%
! AGAAATTAAGTATCTCCTTA+TGG - Chr8:74654241-74654260 MsG0880046546.01.T01:intron 25.0%
! ATAGTTATGATATATAGGTC+TGG - Chr8:74653890-74653909 MsG0880046546.01.T01:intron 25.0%
! ATTGTAAATAATCACACATG+TGG - Chr8:74653640-74653659 MsG0880046546.01.T01:intron 25.0%
! CAAATGAATGTGAAAGAAAA+AGG + Chr8:74654127-74654146 None:intergenic 25.0%
! GCTGTATAGTTATGATATAT+AGG - Chr8:74653885-74653904 MsG0880046546.01.T01:intron 25.0%
! TCAGATGATTTCAATTTCAT+TGG - Chr8:74652811-74652830 MsG0880046546.01.T01:CDS 25.0%
!! TAAGAAGCATAGTAATCAAT+AGG + Chr8:74653808-74653827 None:intergenic 25.0%
!! TTTAGCATCACAATAGTTTT+TGG - Chr8:74653515-74653534 MsG0880046546.01.T01:intron 25.0%
!!! AAAATGGATTATGACTAAAG+AGG + Chr8:74653427-74653446 None:intergenic 25.0%
!!! ATTTTGTTTAAGTCTGGTTT+TGG + Chr8:74653492-74653511 None:intergenic 25.0%
!!! CATGTTTTTGTTTTAAACCT+TGG - Chr8:74653728-74653747 MsG0880046546.01.T01:intron 25.0%
!!! TAATGTTTAGCTTGTTATAC+AGG - Chr8:74653915-74653934 MsG0880046546.01.T01:intron 25.0%
!!! TACATTGATTTCTTTTGTTG+TGG - Chr8:74654169-74654188 MsG0880046546.01.T01:intron 25.0%
AATAACCAAAGGGTTATCAA+AGG + Chr8:74654969-74654988 None:intergenic 30.0%
ACTGCATATTGTCAAGTTTA+TGG - Chr8:74653090-74653109 MsG0880046546.01.T01:CDS 30.0%
ATAAAAGTCTATCAGACACT+CGG + Chr8:74655045-74655064 None:intergenic 30.0%
ATAACCAAAGGGTTATCAAA+GGG + Chr8:74654968-74654987 None:intergenic 30.0%
ATAGTAATCAATAGGTGCAA+AGG + Chr8:74653800-74653819 None:intergenic 30.0%
ATTCCATTCACAATGACTAT+AGG + Chr8:74653189-74653208 None:intergenic 30.0%
ATTTCTAACTAGCTGGAATT+TGG - Chr8:74653322-74653341 MsG0880046546.01.T01:CDS 30.0%
CATTCACAAAATCTGAATCA+TGG + Chr8:74655162-74655181 None:intergenic 30.0%
CGAATGTTGAAAACAAAACA+AGG + Chr8:74653769-74653788 None:intergenic 30.0%
GAAGTTTATGATTGACCATA+AGG + Chr8:74654259-74654278 None:intergenic 30.0%
TAGTAATCAATAGGTGCAAA+GGG + Chr8:74653799-74653818 None:intergenic 30.0%
TATGATTTGATACGATCCTA+TGG - Chr8:74654520-74654539 MsG0880046546.01.T01:intron 30.0%
TCAAGTTTATGGATGTAACA+AGG - Chr8:74653101-74653120 MsG0880046546.01.T01:CDS 30.0%
TCATGTTCTAGTATTCCAAT+AGG + Chr8:74653549-74653568 None:intergenic 30.0%
TCCATCTGTAGGATTTAATT+CGG + Chr8:74655068-74655087 None:intergenic 30.0%
TGAATGGAAACTCAGAATTT+AGG + Chr8:74654330-74654349 None:intergenic 30.0%
TGACTAAAGAGGTTAATGTT+TGG + Chr8:74653416-74653435 None:intergenic 30.0%
TTATTGTCTGGTACCAATAT+AGG + Chr8:74655103-74655122 None:intergenic 30.0%
TTCACGATATCATTATTGTC+TGG + Chr8:74655115-74655134 None:intergenic 30.0%
TTTCAAGCTTTCAAATTCTG+TGG - Chr8:74652906-74652925 MsG0880046546.01.T01:CDS 30.0%
! AAGTGAGTTTTCTGTAAAAG+GGG - Chr8:74652876-74652895 MsG0880046546.01.T01:CDS 30.0%
! ACTAAAAAAGCATAGGAAAC+TGG + Chr8:74654479-74654498 None:intergenic 30.0%
! ACTATACAGCTTCTTATTTG+AGG + Chr8:74653875-74653894 None:intergenic 30.0%
! ATTTTGCATTTCTAACTAGC+TGG - Chr8:74653315-74653334 MsG0880046546.01.T01:CDS 30.0%
! CAAGTGAGTTTTCTGTAAAA+GGG - Chr8:74652875-74652894 MsG0880046546.01.T01:CDS 30.0%
! CTTTTACAGAAAACTCACTT+GGG + Chr8:74652876-74652895 None:intergenic 30.0%
! GCAACATTTTCATGAAGAAA+GGG + Chr8:74654743-74654762 None:intergenic 30.0%
! TAGGCTTTTGTGAAATGTTT+GGG - Chr8:74652779-74652798 MsG0880046546.01.T01:CDS 30.0%
! TCAGATGACTAAAAAAGCAT+AGG + Chr8:74654486-74654505 None:intergenic 30.0%
! TGATATCGTGAACTTTGAAA+TGG - Chr8:74655123-74655142 MsG0880046546.01.T01:CDS 30.0%
! TTAGGCTTTTGTGAAATGTT+TGG - Chr8:74652778-74652797 MsG0880046546.01.T01:CDS 30.0%
! TTTTACATGGTACAACACTA+AGG + Chr8:74654370-74654389 None:intergenic 30.0%
!! AATTGTTTTTCGTTGTACGA+AGG + Chr8:74654719-74654738 None:intergenic 30.0%
!! GAAAAAGGTTCTGAAGAATT+AGG - Chr8:74652760-74652779 MsG0880046546.01.T01:CDS 30.0%
!!! ATGAGCAGGGTTTTAAATTA+TGG - Chr8:74653592-74653611 MsG0880046546.01.T01:intron 30.0%
!!! TTGTTTTAAACCTTGGTCTT+GGG - Chr8:74653735-74653754 MsG0880046546.01.T01:intron 30.0%
!!! TTTGTTTTAAACCTTGGTCT+TGG - Chr8:74653734-74653753 MsG0880046546.01.T01:intron 30.0%
AAGTCATCTCAGTCCTATAT+TGG - Chr8:74655087-74655106 MsG0880046546.01.T01:CDS 35.0%
ACTCCTATAGTCATTGTGAA+TGG - Chr8:74653183-74653202 MsG0880046546.01.T01:CDS 35.0%
AGGGTTGAGCAATAAATTAG+AGG + Chr8:74653376-74653395 None:intergenic 35.0%
CACAAACTTTATGCCTCTTA+TGG + Chr8:74653149-74653168 None:intergenic 35.0%
CGACAGTTGTAGTAAATTGA+TGG + Chr8:74655218-74655237 None:intergenic 35.0%
CTAAGTAGAAACATGACTGA+AGG + Chr8:74653843-74653862 None:intergenic 35.0%
GACTCACAAGAAGACAAAAT+AGG + Chr8:74653033-74653052 None:intergenic 35.0%
GTTATCAAAGGGAATTTCCA+AGG + Chr8:74654957-74654976 None:intergenic 35.0%
GTTGAATTATGTCATGAGCA+GGG - Chr8:74653579-74653598 MsG0880046546.01.T01:intron 35.0%
TCATTCTGTAAGAAAGCAGA+TGG + Chr8:74654281-74654300 None:intergenic 35.0%
TCCGAATTAAATCCTACAGA+TGG - Chr8:74655064-74655083 MsG0880046546.01.T01:CDS 35.0%
TCTTGATTGAAGATGTCCAT+TGG + Chr8:74654687-74654706 None:intergenic 35.0%
TGTTGAATTATGTCATGAGC+AGG - Chr8:74653578-74653597 MsG0880046546.01.T01:intron 35.0%
TTACCATCAAGTTTCTTGCT+TGG + Chr8:74652844-74652863 None:intergenic 35.0%
TTGCATCATCTTCACAAGAA+AGG + Chr8:74655195-74655214 None:intergenic 35.0%
! AATTCCCTTTGATAACCCTT+TGG - Chr8:74654961-74654980 MsG0880046546.01.T01:CDS 35.0%
! ATGTAATTTAATGGGTGCAG+AGG + Chr8:74654154-74654173 None:intergenic 35.0%
! CAAGGATCTTAGTTCTTGTA+AGG - Chr8:74653119-74653138 MsG0880046546.01.T01:CDS 35.0%
! CCAAGTGAGTTTTCTGTAAA+AGG - Chr8:74652874-74652893 MsG0880046546.01.T01:CDS 35.0%
! CCTTTTACAGAAAACTCACT+TGG + Chr8:74652877-74652896 None:intergenic 35.0%
! CTAGGAAGATTTGATGATCA+TGG - Chr8:74652967-74652986 MsG0880046546.01.T01:CDS 35.0%
! CTTCAGTCATGTTTCTACTT+AGG - Chr8:74653841-74653860 MsG0880046546.01.T01:intron 35.0%
! CTTTTTCCCATTTCTAACGA+GGG + Chr8:74654416-74654435 None:intergenic 35.0%
! GGCAACATTTTCATGAAGAA+AGG + Chr8:74654744-74654763 None:intergenic 35.0%
! TAAGAGGCATAAAGTTTGTG+AGG - Chr8:74653149-74653168 MsG0880046546.01.T01:CDS 35.0%
! TCTTTTTCCCATTTCTAACG+AGG + Chr8:74654417-74654436 None:intergenic 35.0%
! TGTAATTTAATGGGTGCAGA+GGG + Chr8:74654153-74654172 None:intergenic 35.0%
! TTTTCGTTGTACGAAGGAAA+CGG + Chr8:74654713-74654732 None:intergenic 35.0%
!! GTGAACTTTGAAATGGAAGA+CGG - Chr8:74655130-74655149 MsG0880046546.01.T01:CDS 35.0%
!! TCTCTCATTGATTTGAAGCT+AGG - Chr8:74652949-74652968 MsG0880046546.01.T01:CDS 35.0%
!! TGAACTTTGAAATGGAAGAC+GGG - Chr8:74655131-74655150 MsG0880046546.01.T01:CDS 35.0%
AATGAGAGAATCCCAACCAT+TGG + Chr8:74652938-74652957 None:intergenic 40.0%
ATGAAAGCAGAACATGAAGC+TGG - Chr8:74654632-74654651 MsG0880046546.01.T01:intron 40.0%
ATTGAAGTCTTCGCTTCCTA+AGG + Chr8:74654834-74654853 None:intergenic 40.0%
CAAGCTTTCAAATTCTGTGG+TGG - Chr8:74652909-74652928 MsG0880046546.01.T01:CDS 40.0%
CACAATGACTATAGGAGTCT+TGG + Chr8:74653181-74653200 None:intergenic 40.0%
CCAAGCATTCAAGGATTATC+AGG - Chr8:74654869-74654888 MsG0880046546.01.T01:CDS 40.0%
CCTGATAATCCTTGAATGCT+TGG + Chr8:74654872-74654891 None:intergenic 40.0%
CTGATATCTGCAAGAACCAT+AGG + Chr8:74654539-74654558 None:intergenic 40.0%
CTTGCAGATATCAGATTCCA+GGG - Chr8:74654545-74654564 MsG0880046546.01.T01:intron 40.0%
CTTGTAAGGACTACCATAAG+AGG - Chr8:74653133-74653152 MsG0880046546.01.T01:CDS 40.0%
GCATCATAGACAATCATTGC+AGG - Chr8:74655258-74655277 MsG0880046546.01.T01:CDS 40.0%
GCGTTCACTCTACAAATTCT+GGG - Chr8:74654026-74654045 MsG0880046546.01.T01:CDS 40.0%
GCTTCATGTTCTGCTTTCAT+AGG + Chr8:74654632-74654651 None:intergenic 40.0%
GTGATCTTATGCTGTAGACA+TGG + Chr8:74655006-74655025 None:intergenic 40.0%
GTTCCAAGCAAGAAACTTGA+TGG - Chr8:74652838-74652857 MsG0880046546.01.T01:CDS 40.0%
TCTTGCAGATATCAGATTCC+AGG - Chr8:74654544-74654563 MsG0880046546.01.T01:intron 40.0%
TGGCAATGCTTCAATTGATG+CGG - Chr8:74652987-74653006 MsG0880046546.01.T01:CDS 40.0%
TTATGCTGTAGACATGGCTA+TGG + Chr8:74655000-74655019 None:intergenic 40.0%
TTCTGTCCCTCGTTAGAAAT+GGG - Chr8:74654407-74654426 MsG0880046546.01.T01:intron 40.0%
TTGCTGTCTGAGTTTGATGA+TGG - Chr8:74653944-74653963 MsG0880046546.01.T01:intron 40.0%
TTGTCAACAATGTAGCAGGT+TGG - Chr8:74653221-74653240 MsG0880046546.01.T01:CDS 40.0%
TTGTGAATGGAATTGAGCAG+AGG - Chr8:74653196-74653215 MsG0880046546.01.T01:CDS 40.0%
TTTCTGTCCCTCGTTAGAAA+TGG - Chr8:74654406-74654425 MsG0880046546.01.T01:intron 40.0%
! AATTCTGTGGTGGAATCCAA+TGG - Chr8:74652919-74652938 MsG0880046546.01.T01:CDS 40.0%
! AGCATCTTTTCTCTATCCAG+AGG - Chr8:74654583-74654602 MsG0880046546.01.T01:intron 40.0%
! ATTGATGCGGCATTTTCCAA+AGG - Chr8:74653000-74653019 MsG0880046546.01.T01:CDS 40.0%
! GTTTTCTTCGACGCTCATTA+TGG + Chr8:74654000-74654019 None:intergenic 40.0%
! TAATCCTTGAATGCTTGGTG+CGG + Chr8:74654867-74654886 None:intergenic 40.0%
!! GGTTTTGTCAACAATGTAGC+AGG - Chr8:74653217-74653236 MsG0880046546.01.T01:CDS 40.0%
!! TTTTTGGTGTGTGTGCCTAT+TGG - Chr8:74653531-74653550 MsG0880046546.01.T01:intron 40.0%
AATGTTGCCACATCCACTAC+AGG - Chr8:74654755-74654774 MsG0880046546.01.T01:intron 45.0%
ACTGAACTTACTGCCTGTAG+TGG + Chr8:74654771-74654790 None:intergenic 45.0%
AGACTGTTTCAGCCATTTGG+TGG - Chr8:74654049-74654068 MsG0880046546.01.T01:CDS 45.0%
AGGTCTTCCAAAGTGGTTTC+TGG - Chr8:74654603-74654622 MsG0880046546.01.T01:intron 45.0%
CAAAGGGAATTTCCAAGGAC+TGG + Chr8:74654952-74654971 None:intergenic 45.0%
CATGTAAAATGCCGCTGACA+CGG - Chr8:74654381-74654400 MsG0880046546.01.T01:intron 45.0%
CTGAGATGACTTCCATCTGT+AGG + Chr8:74655079-74655098 None:intergenic 45.0%
GGATAGAGAAAAGATGCTGC+TGG + Chr8:74654581-74654600 None:intergenic 45.0%
GGCGTTCACTCTACAAATTC+TGG - Chr8:74654025-74654044 MsG0880046546.01.T01:CDS 45.0%
GGCTGCTTGGAATAACCAAA+GGG + Chr8:74654979-74654998 None:intergenic 45.0%
TAACACTTCAAGCCAGTCCT+TGG - Chr8:74654937-74654956 MsG0880046546.01.T01:CDS 45.0%
TAGAGAAAAGATGCTGCTGG+TGG + Chr8:74654578-74654597 None:intergenic 45.0%
TAGGTCTCCAGAAACCACTT+TGG + Chr8:74654613-74654632 None:intergenic 45.0%
TATCCAGAGGTCTTCCAAAG+TGG - Chr8:74654596-74654615 MsG0880046546.01.T01:intron 45.0%
TATGGCCTGCAAGTCGTTTA+CGG + Chr8:74653982-74654001 None:intergenic 45.0%
TGGCTGCTTGGAATAACCAA+AGG + Chr8:74654980-74654999 None:intergenic 45.0%
TTTGTAGAGTGAACGCCTTG+TGG + Chr8:74654022-74654041 None:intergenic 45.0%
! AAACCACTTTGGAAGACCTC+TGG + Chr8:74654602-74654621 None:intergenic 45.0%
! TGAAGATGTCCATTGGTGAC+AGG + Chr8:74654680-74654699 None:intergenic 45.0%
! TGTGGTGGAATCCAATGGTT+GGG - Chr8:74652924-74652943 MsG0880046546.01.T01:CDS 45.0%
!! CAAAATAGGTGCTGCTCCTT+TGG + Chr8:74653019-74653038 None:intergenic 45.0%
!! CAGAACATGAAGCTGGCTTT+AGG - Chr8:74654639-74654658 MsG0880046546.01.T01:intron 45.0%
!! GGACTGGCTTGAAGTGTTAT+TGG + Chr8:74654936-74654955 None:intergenic 45.0%
AAAAGATGCTGCTGGTGGCT+TGG + Chr8:74654573-74654592 None:intergenic 50.0%
AGCCAATGGCATGCGCATAA+TGG + Chr8:74654805-74654824 None:intergenic 50.0%
AGCCATTATGCGCATGCCAT+TGG - Chr8:74654800-74654819 MsG0880046546.01.T01:intron 50.0%
AGTTGCCGTAAACGACTTGC+AGG - Chr8:74653974-74653993 MsG0880046546.01.T01:intron 50.0%
CATACCGCACCAAGCATTCA+AGG - Chr8:74654860-74654879 MsG0880046546.01.T01:CDS 50.0%
GAAGTCTTCGCTTCCTAAGG+AGG + Chr8:74654831-74654850 None:intergenic 50.0%
GAGTGCACTCTCACTCTCTT+GGG + Chr8:74653066-74653085 None:intergenic 50.0%
GGACAGAAAATCCGTGTCAG+CGG + Chr8:74654395-74654414 None:intergenic 50.0%
GGCTTCAAAACCCAAGACCA+AGG + Chr8:74653748-74653767 None:intergenic 50.0%
GGGAGACTGTTTCAGCCATT+TGG - Chr8:74654046-74654065 MsG0880046546.01.T01:CDS 50.0%
TAGACATGGCTATGGCTGCT+TGG + Chr8:74654992-74655011 None:intergenic 50.0%
TCTTCTGTGCCTGTCACCAA+TGG - Chr8:74654668-74654687 MsG0880046546.01.T01:intron 50.0%
TGAGTGCACTCTCACTCTCT+TGG + Chr8:74653067-74653086 None:intergenic 50.0%
! CGTGTCAGCGGCATTTTACA+TGG + Chr8:74654383-74654402 None:intergenic 50.0%
! CTGTGGTGGAATCCAATGGT+TGG - Chr8:74652923-74652942 MsG0880046546.01.T01:CDS 50.0%
! TCCTAAGGAGGTGTAGCCAA+TGG + Chr8:74654819-74654838 None:intergenic 50.0%
! TCTATCAGACACTCGGCTTG+AGG + Chr8:74655038-74655057 None:intergenic 50.0%
!! CTTACTGCCTGTAGTGGATG+TGG + Chr8:74654765-74654784 None:intergenic 50.0%
!! GAGCGTCGAAGAAAACCACA+AGG - Chr8:74654004-74654023 MsG0880046546.01.T01:CDS 50.0%
TGCACTCTCACTCTCTTGGG+AGG + Chr8:74653063-74653082 None:intergenic 55.0%
! GCCATTGGCTACACCTCCTT+AGG - Chr8:74654815-74654834 MsG0880046546.01.T01:intron 55.0%
!! CTGCTGGTGGCTTGGTTCCC+TGG + Chr8:74654565-74654584 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 74652733 74655324 74652733 ID=MsG0880046546.01;Name=MsG0880046546.01
Chr8 mRNA 74652733 74655324 74652733 ID=MsG0880046546.01.T01;Parent=MsG0880046546.01;Name=MsG0880046546.01.T01;_AED=0.39;_eAED=0.39;_QI=0|0|0|1|1|1|3|0|470
Chr8 exon 74654819 74655324 74654819 ID=MsG0880046546.01.T01:exon:31174;Parent=MsG0880046546.01.T01
Chr8 exon 74653987 74654120 74653987 ID=MsG0880046546.01.T01:exon:31173;Parent=MsG0880046546.01.T01
Chr8 exon 74652733 74653505 74652733 ID=MsG0880046546.01.T01:exon:31172;Parent=MsG0880046546.01.T01
Chr8 CDS 74654819 74655324 74654819 ID=MsG0880046546.01.T01:cds;Parent=MsG0880046546.01.T01
Chr8 CDS 74653987 74654120 74653987 ID=MsG0880046546.01.T01:cds;Parent=MsG0880046546.01.T01
Chr8 CDS 74652733 74653505 74652733 ID=MsG0880046546.01.T01:cds;Parent=MsG0880046546.01.T01
Gene Sequence

>MsG0880046546.01.T01

ATGGAATCTTGGAGTTATATTTCTGAAGAAAAAGGTTCTGAAGAATTAGGCTTTTGTGAAATGTTTGGGAAACAATATTCAGATGATTTCAATTTCATTGGAAGTGTTCCAAGCAAGAAACTTGATGGTAAATTTGATGACCCAAGTGAGTTTTCTGTAAAAGGGGACACAAATTTCAAGCTTTCAAATTCTGTGGTGGAATCCAATGGTTGGGATTCTCTCATTGATTTGAAGCTAGGAAGATTTGATGATCATGGCAATGCTTCAATTGATGCGGCATTTTCCAAAGGAGCAGCACCTATTTTGTCTTCTTGTGAGTCTTCAACACCTCCCAAGAGAGTGAGAGTGCACTCAATGACTGCATATTGTCAAGTTTATGGATGTAACAAGGATCTTAGTTCTTGTAAGGACTACCATAAGAGGCATAAAGTTTGTGAGGTTCACTCCAAGACTCCTATAGTCATTGTGAATGGAATTGAGCAGAGGTTTTGTCAACAATGTAGCAGGTTTCATTTGCTGTCTGAGTTTGATGATGGTAAGCGCAGTTGCCGTAAACGACTTGCAGGCCATAATGAGCGTCGAAGAAAACCACAAGGCGTTCACTCTACAAATTCTGGGAGACTGTTTCAGCCATTTGGTGATATCAGATTCCAGGGAACCAAGCCACCAGCAGCATCTTTTCTCTATCCAGAGGTCTTCCAAAGTGGTTTCTGGAGACCTATGAAAGCAGAACATGAAGCTGGCTTTAGGCATTTTTCTTCTGTGCCTGTCACCAATGGACATCTTCAATCAAGATCTCCGTTTCCTTCGTACAACGAAAAACAATTTCCCTTTCTTCATGAAAATGTTGCCACATCCACTACAGGCAGTAAGTTCAGTGAAAACAATAGCCATTATGCGCATGCCATTGGCTACACCTCCTTAGGAAGCGAAGACTTCAATGTTTTTCATACCGCACCAAGCATTCAAGGATTATCAGGAATTTCAGACTCTTGTGCTCTCTCTCTTCTGTCATCTCAGTCCAATAACACTTCAAGCCAGTCCTTGGAAATTCCCTTTGATAACCCTTTGGTTATTCCAAGCAGCCATAGCCATGTCTACAGCATAAGATCACAAGCGACTTCCTCAAGCCGAGTGTCTGATAGACTTTTATCCGAATTAAATCCTACAGATGGAAGTCATCTCAGTCCTATATTGGTACCAGACAATAATGATATCGTGAACTTTGAAATGGAAGACGGGATCTTCCATGATTCAGATTTTGTGAATGCGAAAGACTGCCTTTCTTGTGAAGATGATGCAACCATCAATTTACTACAACTGTCGTCGCAGCTTCAACGAGTTGAGCATCATAGACAATCATTGCAGGTGAAGAAAGAGTTTGATTCTTCTTGCACTCTCCGAATTACTTAA
Protein sequence

>MsG0880046546.01.T01

MESWSYISEEKGSEELGFCEMFGKQYSDDFNFIGSVPSKKLDGKFDDPSEFSVKGDTNFKLSNSVVESNGWDSLIDLKLGRFDDHGNASIDAAFSKGAAPILSSCESSTPPKRVRVHSMTAYCQVYGCNKDLSSCKDYHKRHKVCEVHSKTPIVIVNGIEQRFCQQCSRFHLLSEFDDGKRSCRKRLAGHNERRRKPQGVHSTNSGRLFQPFGDIRFQGTKPPAASFLYPEVFQSGFWRPMKAEHEAGFRHFSSVPVTNGHLQSRSPFPSYNEKQFPFLHENVATSTTGSKFSENNSHYAHAIGYTSLGSEDFNVFHTAPSIQGLSGISDSCALSLLSSQSNNTSSQSLEIPFDNPLVIPSSHSHVYSIRSQATSSSRVSDRLLSELNPTDGSHLSPILVPDNNDIVNFEMEDGIFHDSDFVNAKDCLSCEDDATINLLQLSSQLQRVEHHRQSLQVKKEFDSSCTLRIT*