Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046776.01.T01 | KEH15779.1 | 86.441 | 295 | 39 | 1 | 1 | 294 | 1 | 295 | 0 | 539 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046776.01.T01 | Q9SZL8 | 28.788 | 264 | 171 | 4 | 32 | 295 | 70 | 316 | 8.72E-26 | 111 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046776.01.T01 | A0A072TE13 | 86.441 | 295 | 39 | 1 | 1 | 294 | 1 | 295 | 0.0 | 539 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0880046776.01.T01 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046776.01.T01 | MTR_0558s0020 | 86.441 | 295 | 39 | 1 | 1 | 294 | 1 | 295 | 0.0 | 539 |
| MsG0880046776.01.T01 | MTR_1g083640 | 82.042 | 284 | 35 | 1 | 1 | 268 | 1 | 284 | 3.24e-176 | 489 |
| MsG0880046776.01.T01 | MTR_8g446020 | 72.789 | 294 | 41 | 1 | 1 | 294 | 1 | 255 | 1.30e-155 | 436 |
| MsG0880046776.01.T01 | MTR_2g014100 | 81.633 | 245 | 36 | 1 | 1 | 236 | 1 | 245 | 9.28e-148 | 416 |
| MsG0880046776.01.T01 | MTR_6g090505 | 84.348 | 230 | 36 | 0 | 65 | 294 | 1 | 230 | 5.13e-146 | 416 |
| MsG0880046776.01.T01 | MTR_0337s0040 | 71.815 | 259 | 33 | 2 | 1 | 258 | 1 | 220 | 3.82e-128 | 365 |
| MsG0880046776.01.T01 | MTR_3g008240 | 84.236 | 203 | 31 | 1 | 1 | 202 | 1 | 203 | 4.08e-123 | 352 |
| MsG0880046776.01.T01 | MTR_4g040430 | 83.168 | 202 | 30 | 1 | 1 | 202 | 1 | 198 | 9.29e-122 | 348 |
| MsG0880046776.01.T01 | MTR_5g071590 | 73.333 | 180 | 35 | 2 | 1 | 167 | 1 | 180 | 7.23e-94 | 277 |
| MsG0880046776.01.T01 | MTR_1g055240 | 86.620 | 142 | 19 | 0 | 91 | 232 | 3 | 144 | 1.41e-89 | 265 |
| MsG0880046776.01.T01 | MTR_0019s0290 | 80.921 | 152 | 29 | 0 | 82 | 233 | 9 | 160 | 1.48e-88 | 263 |
| MsG0880046776.01.T01 | MTR_1281s0010 | 79.817 | 109 | 22 | 0 | 19 | 127 | 1 | 109 | 2.10e-59 | 186 |
| MsG0880046776.01.T01 | MTR_4g008820 | 83.750 | 80 | 12 | 1 | 1 | 79 | 125 | 204 | 3.88e-34 | 132 |
| MsG0880046776.01.T01 | MTR_8g046060 | 77.273 | 88 | 11 | 2 | 1 | 79 | 91 | 178 | 2.58e-33 | 122 |
| MsG0880046776.01.T01 | MTR_6g051425 | 31.325 | 249 | 156 | 4 | 40 | 288 | 22 | 255 | 3.03e-31 | 120 |
| MsG0880046776.01.T01 | MTR_1g070355 | 28.287 | 251 | 161 | 4 | 41 | 291 | 80 | 311 | 3.53e-20 | 91.7 |
| MsG0880046776.01.T01 | MTR_4g035820 | 22.654 | 309 | 205 | 8 | 17 | 317 | 61 | 343 | 2.32e-15 | 77.4 |
| MsG0880046776.01.T01 | MTR_0402s0040 | 25.405 | 185 | 119 | 4 | 49 | 233 | 40 | 205 | 4.43e-15 | 76.3 |
| MsG0880046776.01.T01 | MTR_0337s0050 | 67.500 | 40 | 13 | 0 | 259 | 298 | 14 | 53 | 1.52e-14 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046776.01.T01 | AT4G38180 | 28.788 | 264 | 171 | 4 | 32 | 295 | 70 | 316 | 8.89e-27 | 111 |
| MsG0880046776.01.T01 | AT5G28530 | 23.372 | 261 | 168 | 7 | 40 | 287 | 60 | 301 | 1.03e-14 | 75.5 |
| MsG0880046776.01.T01 | AT5G28530 | 23.372 | 261 | 168 | 7 | 40 | 287 | 60 | 301 | 1.03e-14 | 75.5 |
| MsG0880046776.01.T01 | AT5G28530 | 23.372 | 261 | 168 | 7 | 40 | 287 | 60 | 301 | 1.05e-14 | 75.5 |
| MsG0880046776.01.T01 | AT1G10240 | 24.609 | 256 | 163 | 9 | 49 | 296 | 62 | 295 | 4.05e-14 | 73.6 |
| MsG0880046776.01.T01 | AT1G10240 | 24.609 | 256 | 163 | 9 | 49 | 296 | 62 | 295 | 4.05e-14 | 73.6 |
| MsG0880046776.01.T01 | AT2G27110 | 26.901 | 171 | 108 | 4 | 127 | 295 | 108 | 263 | 4.19e-12 | 67.8 |
| MsG0880046776.01.T01 | AT2G27110 | 26.901 | 171 | 108 | 4 | 127 | 295 | 108 | 263 | 4.19e-12 | 67.8 |
| MsG0880046776.01.T01 | AT1G76320 | 21.908 | 283 | 189 | 6 | 41 | 321 | 3 | 255 | 7.26e-12 | 66.6 |
| MsG0880046776.01.T01 | AT1G76320 | 21.908 | 283 | 189 | 6 | 41 | 321 | 3 | 255 | 7.95e-12 | 66.6 |
| MsG0880046776.01.T01 | AT1G76320 | 21.908 | 283 | 189 | 6 | 41 | 321 | 3 | 255 | 8.33e-12 | 66.6 |
| MsG0880046776.01.T01 | AT1G76320 | 21.908 | 283 | 189 | 6 | 41 | 321 | 3 | 255 | 8.33e-12 | 66.6 |
| MsG0880046776.01.T01 | AT4G15090 | 25.403 | 248 | 164 | 6 | 50 | 296 | 66 | 293 | 8.63e-12 | 66.6 |
| MsG0880046776.01.T01 | AT4G15090 | 25.403 | 248 | 164 | 6 | 50 | 296 | 66 | 293 | 8.63e-12 | 66.6 |
| MsG0880046776.01.T01 | AT4G15090 | 25.403 | 248 | 164 | 6 | 50 | 296 | 66 | 293 | 8.63e-12 | 66.6 |
| MsG0880046776.01.T01 | AT4G15090 | 25.403 | 248 | 164 | 6 | 50 | 296 | 66 | 293 | 8.63e-12 | 66.6 |
| MsG0880046776.01.T01 | AT4G15090 | 25.403 | 248 | 164 | 6 | 50 | 296 | 66 | 293 | 8.63e-12 | 66.6 |
| MsG0880046776.01.T01 | AT2G27110 | 35.366 | 82 | 53 | 0 | 213 | 294 | 36 | 117 | 2.85e-11 | 65.1 |
Find 45 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACAAGATTATGCTCTATT+TGG | 0.198611 | 8:-77955440 | MsG0880046776.01.T01:CDS |
| TATCGGCCGTTTACAGTAAC+TGG | 0.299041 | 8:-77955374 | MsG0880046776.01.T01:CDS |
| CCTTATTCTGAAAATTTGTA+TGG | 0.314405 | 8:-77955290 | MsG0880046776.01.T01:CDS |
| GCCGATATTTATTGTTTGTT+CGG | 0.361178 | 8:+77955390 | None:intergenic |
| TTCTCATACTCAACAAGAAC+TGG | 0.395633 | 8:+77955920 | None:intergenic |
| CCATGATTCTTTATCACTAA+AGG | 0.406174 | 8:+77956159 | None:intergenic |
| ACCGAACAAACAATAAATAT+CGG | 0.406375 | 8:-77955391 | MsG0880046776.01.T01:CDS |
| TAAAGAATCATGGAAGTCTT+TGG | 0.408500 | 8:-77956149 | MsG0880046776.01.T01:CDS |
| TCGTTTCCAGTTACTGTAAA+CGG | 0.409191 | 8:+77955368 | None:intergenic |
| ATAACACTGGATTCTCTGTT+AGG | 0.417808 | 8:-77956078 | MsG0880046776.01.T01:CDS |
| CGAGAAGTAAATGAAGTTCA+AGG | 0.430014 | 8:-77955743 | MsG0880046776.01.T01:CDS |
| AGCTGGAATATATTTGAAGT+TGG | 0.433136 | 8:-77955861 | MsG0880046776.01.T01:CDS |
| TGAATGTCTATAAGATATAT+AGG | 0.445219 | 8:-77955829 | MsG0880046776.01.T01:CDS |
| GAAAGGGTTGGTTGTAAAGC+TGG | 0.447509 | 8:-77955878 | MsG0880046776.01.T01:CDS |
| CGTTATGCTTGTAACAAAGA+AGG | 0.449439 | 8:-77955995 | MsG0880046776.01.T01:CDS |
| GCTTTAATAAATTCTAAAGC+TGG | 0.450264 | 8:-77955713 | MsG0880046776.01.T01:CDS |
| CTCAAGTAGCGCAAATGCTC+TGG | 0.460440 | 8:+77955773 | None:intergenic |
| ACAAAATAAGAAATCGGATA+AGG | 0.469178 | 8:-77956029 | MsG0880046776.01.T01:CDS |
| ATCCGAGAGTGGACAATTGA+CGG | 0.474201 | 8:-77956224 | MsG0880046776.01.T01:CDS |
| GAAGTGATGGGTCAAGGAGT+TGG | 0.484292 | 8:-77955668 | MsG0880046776.01.T01:CDS |
| TTACAACCAACCCTTTCCTC+TGG | 0.485330 | 8:+77955884 | None:intergenic |
| TTTAATTATGCATATAACAC+TGG | 0.485542 | 8:-77956091 | MsG0880046776.01.T01:CDS |
| TGTCTTCTGCAGCATCAACT+TGG | 0.487243 | 8:+77955495 | None:intergenic |
| CAATTCACAAAATAAGAAAT+CGG | 0.490263 | 8:-77956035 | MsG0880046776.01.T01:CDS |
| GAGAACATCTTATGAAGTGA+TGG | 0.499875 | 8:-77955681 | MsG0880046776.01.T01:CDS |
| AACCGTCAATTGTCCACTCT+CGG | 0.506734 | 8:+77956222 | None:intergenic |
| TTTAAGTCCGAAAATCGAAA+AGG | 0.508080 | 8:+77955629 | None:intergenic |
| TGTCTATAAGATATATAGGT+GGG | 0.523740 | 8:-77955825 | MsG0880046776.01.T01:CDS |
| TAACAAAGAAGGTTTCAAGA+AGG | 0.538422 | 8:-77955984 | MsG0880046776.01.T01:CDS |
| CGTCAAAAGAAGATGGTGGC+TGG | 0.540133 | 8:-77955587 | MsG0880046776.01.T01:CDS |
| CCTTTAGTGATAAAGAATCA+TGG | 0.543522 | 8:-77956159 | MsG0880046776.01.T01:CDS |
| AACAAAGAAGGTTTCAAGAA+GGG | 0.545537 | 8:-77955983 | MsG0880046776.01.T01:CDS |
| CTTATTCTGAAAATTTGTAT+GGG | 0.551924 | 8:-77955289 | MsG0880046776.01.T01:CDS |
| GACAACTCGTCAAAAGAAGA+TGG | 0.552238 | 8:-77955594 | MsG0880046776.01.T01:CDS |
| AAATGCTCTGGTTTATGTAA+TGG | 0.552634 | 8:+77955785 | None:intergenic |
| AATGAAGCTCTCTCGAAACC+AGG | 0.565261 | 8:-77955533 | MsG0880046776.01.T01:CDS |
| TGAAAATTCAGTTATTGATG+CGG | 0.565546 | 8:-77956200 | MsG0880046776.01.T01:CDS |
| GTGATGGGTCAAGGAGTTGG+AGG | 0.568802 | 8:-77955665 | MsG0880046776.01.T01:CDS |
| TCTTATGAAGTGATGGGTCA+AGG | 0.589029 | 8:-77955674 | MsG0880046776.01.T01:CDS |
| AGAACATCTTATGAAGTGAT+GGG | 0.601988 | 8:-77955680 | MsG0880046776.01.T01:CDS |
| ATATCAACTTCATCCGAGAG+TGG | 0.612216 | 8:-77956235 | MsG0880046776.01.T01:CDS |
| AGAACTGGACTGTCACATGT+TGG | 0.620791 | 8:+77955935 | None:intergenic |
| GGGTCAAGGAGTTGGAGGCA+CGG | 0.621882 | 8:-77955660 | MsG0880046776.01.T01:CDS |
| ATGTCTATAAGATATATAGG+TGG | 0.643489 | 8:-77955826 | MsG0880046776.01.T01:CDS |
| AACTCGTCAAAAGAAGATGG+TGG | 0.679247 | 8:-77955591 | MsG0880046776.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAATTCACAAAATAAGAAAT+CGG | - | Chr8:77955477-77955496 | MsG0880046776.01.T01:CDS | 20.0% |
| !! | TGAATGTCTATAAGATATAT+AGG | - | Chr8:77955683-77955702 | MsG0880046776.01.T01:CDS | 20.0% |
| !! | TTTAATTATGCATATAACAC+TGG | - | Chr8:77955421-77955440 | MsG0880046776.01.T01:CDS | 20.0% |
| !!! | AAGACATAACTAGTATTTTT+TGG | - | Chr8:77956034-77956053 | MsG0880046776.01.T01:CDS | 20.0% |
| !!! | AGACATAACTAGTATTTTTT+GGG | - | Chr8:77956035-77956054 | MsG0880046776.01.T01:CDS | 20.0% |
| !!! | CTTATTCTGAAAATTTGTAT+GGG | - | Chr8:77956223-77956242 | MsG0880046776.01.T01:CDS | 20.0% |
| ! | ACAAAATAAGAAATCGGATA+AGG | - | Chr8:77955483-77955502 | MsG0880046776.01.T01:CDS | 25.0% |
| ! | ACCGAACAAACAATAAATAT+CGG | - | Chr8:77956121-77956140 | MsG0880046776.01.T01:CDS | 25.0% |
| ! | ATGTCTATAAGATATATAGG+TGG | - | Chr8:77955686-77955705 | MsG0880046776.01.T01:CDS | 25.0% |
| ! | TGAAAATTCAGTTATTGATG+CGG | - | Chr8:77955312-77955331 | MsG0880046776.01.T01:CDS | 25.0% |
| ! | TGTCTATAAGATATATAGGT+GGG | - | Chr8:77955687-77955706 | MsG0880046776.01.T01:CDS | 25.0% |
| !! | CCATACAAATTTTCAGAATA+AGG | + | Chr8:77956225-77956244 | None:intergenic | 25.0% |
| !! | CCTTATTCTGAAAATTTGTA+TGG | - | Chr8:77956222-77956241 | MsG0880046776.01.T01:CDS | 25.0% |
| !! | GAATTTTCCTTTTCGATTTT+CGG | - | Chr8:77955876-77955895 | MsG0880046776.01.T01:CDS | 25.0% |
| !!! | CTTATTTTGTGAATTGTTCT+TGG | + | Chr8:77955473-77955492 | None:intergenic | 25.0% |
| !!! | GCTTTAATAAATTCTAAAGC+TGG | - | Chr8:77955799-77955818 | MsG0880046776.01.T01:CDS | 25.0% |
| AACAAAGAAGGTTTCAAGAA+GGG | - | Chr8:77955529-77955548 | MsG0880046776.01.T01:CDS | 30.0% | |
| AGAACATCTTATGAAGTGAT+GGG | - | Chr8:77955832-77955851 | MsG0880046776.01.T01:CDS | 30.0% | |
| CAACAAGATTATGCTCTATT+TGG | - | Chr8:77956072-77956091 | MsG0880046776.01.T01:CDS | 30.0% | |
| CCATGATTCTTTATCACTAA+AGG | + | Chr8:77955356-77955375 | None:intergenic | 30.0% | |
| CCTTTAGTGATAAAGAATCA+TGG | - | Chr8:77955353-77955372 | MsG0880046776.01.T01:CDS | 30.0% | |
| GCCGATATTTATTGTTTGTT+CGG | + | Chr8:77956125-77956144 | None:intergenic | 30.0% | |
| TAAAGAAAAACCAGAGGAAA+GGG | - | Chr8:77955618-77955637 | MsG0880046776.01.T01:CDS | 30.0% | |
| TAAAGAATCATGGAAGTCTT+TGG | - | Chr8:77955363-77955382 | MsG0880046776.01.T01:CDS | 30.0% | |
| TAACAAAGAAGGTTTCAAGA+AGG | - | Chr8:77955528-77955547 | MsG0880046776.01.T01:CDS | 30.0% | |
| TGGATATCATAGTAAAAACC+TGG | + | Chr8:77956000-77956019 | None:intergenic | 30.0% | |
| TTTAAGTCCGAAAATCGAAA+AGG | + | Chr8:77955886-77955905 | None:intergenic | 30.0% | |
| ! | AAATGCTCTGGTTTATGTAA+TGG | + | Chr8:77955730-77955749 | None:intergenic | 30.0% |
| ! | AGCTGGAATATATTTGAAGT+TGG | - | Chr8:77955651-77955670 | MsG0880046776.01.T01:CDS | 30.0% |
| !!! | ACGTCTGACATTTTTTGTTT+TGG | - | Chr8:77956180-77956199 | MsG0880046776.01.T01:CDS | 30.0% |
| ATAACACTGGATTCTCTGTT+AGG | - | Chr8:77955434-77955453 | MsG0880046776.01.T01:CDS | 35.0% | |
| CGAGAAGTAAATGAAGTTCA+AGG | - | Chr8:77955769-77955788 | MsG0880046776.01.T01:CDS | 35.0% | |
| CGTTATGCTTGTAACAAAGA+AGG | - | Chr8:77955517-77955536 | MsG0880046776.01.T01:CDS | 35.0% | |
| CTAAAGAAAAACCAGAGGAA+AGG | - | Chr8:77955617-77955636 | MsG0880046776.01.T01:CDS | 35.0% | |
| GAAAGCTAAAGAAAAACCAG+AGG | - | Chr8:77955612-77955631 | MsG0880046776.01.T01:CDS | 35.0% | |
| GAGAACATCTTATGAAGTGA+TGG | - | Chr8:77955831-77955850 | MsG0880046776.01.T01:CDS | 35.0% | |
| GTCTGCTTAATCCTAAAAAC+AGG | - | Chr8:77955554-77955573 | MsG0880046776.01.T01:CDS | 35.0% | |
| TCGTTTCCAGTTACTGTAAA+CGG | + | Chr8:77956147-77956166 | None:intergenic | 35.0% | |
| TTCTCATACTCAACAAGAAC+TGG | + | Chr8:77955595-77955614 | None:intergenic | 35.0% | |
| !!! | ACTAGTATTTTTTGGGCAGA+TGG | - | Chr8:77956042-77956061 | MsG0880046776.01.T01:CDS | 35.0% |
| AACTCGTCAAAAGAAGATGG+TGG | - | Chr8:77955921-77955940 | MsG0880046776.01.T01:CDS | 40.0% | |
| ATATCAACTTCATCCGAGAG+TGG | - | Chr8:77955277-77955296 | MsG0880046776.01.T01:CDS | 40.0% | |
| GACAACTCGTCAAAAGAAGA+TGG | - | Chr8:77955918-77955937 | MsG0880046776.01.T01:CDS | 40.0% | |
| !! | TCACATGTTGGCCTGTTTTT+AGG | + | Chr8:77955568-77955587 | None:intergenic | 40.0% |
| !! | TCTTATGAAGTGATGGGTCA+AGG | - | Chr8:77955838-77955857 | MsG0880046776.01.T01:CDS | 40.0% |
| AACCGTCAATTGTCCACTCT+CGG | + | Chr8:77955293-77955312 | None:intergenic | 45.0% | |
| AATGAAGCTCTCTCGAAACC+AGG | - | Chr8:77955979-77955998 | MsG0880046776.01.T01:CDS | 45.0% | |
| AGAACTGGACTGTCACATGT+TGG | + | Chr8:77955580-77955599 | None:intergenic | 45.0% | |
| ATCCGAGAGTGGACAATTGA+CGG | - | Chr8:77955288-77955307 | MsG0880046776.01.T01:CDS | 45.0% | |
| GAAAAACCAGAGGAAAGGGT+TGG | - | Chr8:77955622-77955641 | MsG0880046776.01.T01:CDS | 45.0% | |
| TATCGGCCGTTTACAGTAAC+TGG | - | Chr8:77956138-77956157 | MsG0880046776.01.T01:CDS | 45.0% | |
| TGTCTTCTGCAGCATCAACT+TGG | + | Chr8:77956020-77956039 | None:intergenic | 45.0% | |
| TTACAACCAACCCTTTCCTC+TGG | + | Chr8:77955631-77955650 | None:intergenic | 45.0% | |
| !! | GAAAGGGTTGGTTGTAAAGC+TGG | - | Chr8:77955634-77955653 | MsG0880046776.01.T01:CDS | 45.0% |
| CTCAAGTAGCGCAAATGCTC+TGG | + | Chr8:77955742-77955761 | None:intergenic | 50.0% | |
| ! | CGTCAAAAGAAGATGGTGGC+TGG | - | Chr8:77955925-77955944 | MsG0880046776.01.T01:CDS | 50.0% |
| !! | GAAGTGATGGGTCAAGGAGT+TGG | - | Chr8:77955844-77955863 | MsG0880046776.01.T01:CDS | 50.0% |
| !! | GTGATGGGTCAAGGAGTTGG+AGG | - | Chr8:77955847-77955866 | MsG0880046776.01.T01:CDS | 55.0% |
| GGGTCAAGGAGTTGGAGGCA+CGG | - | Chr8:77955852-77955871 | MsG0880046776.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 77955268 | 77956266 | 77955268 | ID=MsG0880046776.01;Name=MsG0880046776.01 |
| Chr8 | mRNA | 77955268 | 77956266 | 77955268 | ID=MsG0880046776.01.T01;Parent=MsG0880046776.01;Name=MsG0880046776.01.T01;_AED=0.06;_eAED=0.06;_QI=0|-1|0|1|-1|1|1|0|332 |
| Chr8 | exon | 77955268 | 77956266 | 77955268 | ID=MsG0880046776.01.T01:exon:18576;Parent=MsG0880046776.01.T01 |
| Chr8 | CDS | 77955268 | 77956266 | 77955268 | ID=MsG0880046776.01.T01:cds;Parent=MsG0880046776.01.T01 |
| Gene Sequence |
| Protein sequence |
