Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046919.01.T01 | XP_013458150.1 | 90.536 | 317 | 12 | 3 | 323 | 637 | 401 | 701 | 0 | 589 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046919.01.T01 | A0A072US50 | 90.536 | 317 | 12 | 3 | 323 | 637 | 401 | 701 | 0.0 | 589 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046919.01.T01 | MTR_4g117040 | 90.536 | 317 | 12 | 3 | 323 | 637 | 401 | 701 | 0.0 | 589 |
| MsG0880046919.01.T01 | MTR_4g117020 | 80.186 | 323 | 45 | 4 | 323 | 643 | 402 | 707 | 8.11e-178 | 524 |
| MsG0880046919.01.T01 | MTR_4g117030 | 81.388 | 317 | 40 | 4 | 323 | 637 | 363 | 662 | 5.32e-176 | 518 |
| MsG0880046919.01.T01 | MTR_4g117050 | 74.206 | 252 | 40 | 5 | 1 | 251 | 1 | 228 | 2.84e-118 | 352 |
| MsG0880046919.01.T01 | MTR_8g051640 | 54.369 | 309 | 121 | 4 | 345 | 648 | 420 | 713 | 6.16e-102 | 327 |
| MsG0880046919.01.T01 | MTR_8g051540 | 56.711 | 298 | 108 | 4 | 345 | 637 | 454 | 735 | 8.42e-102 | 328 |
| MsG0880046919.01.T01 | MTR_8g442370 | 56.040 | 298 | 110 | 4 | 345 | 637 | 442 | 723 | 7.23e-101 | 326 |
| MsG0880046919.01.T01 | MTR_8g442270 | 56.747 | 289 | 107 | 3 | 351 | 637 | 461 | 733 | 1.09e-100 | 325 |
| MsG0880046919.01.T01 | MTR_8g442310 | 56.711 | 298 | 108 | 5 | 345 | 637 | 408 | 689 | 2.54e-100 | 323 |
| MsG0880046919.01.T01 | MTR_8g442340 | 55.705 | 298 | 111 | 4 | 345 | 637 | 395 | 676 | 4.92e-100 | 322 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046919.01.T01 | AT3G16030 | 53.767 | 292 | 115 | 4 | 350 | 637 | 385 | 660 | 2.35e-97 | 315 |
| MsG0880046919.01.T01 | AT4G23180 | 54.167 | 288 | 114 | 3 | 352 | 637 | 331 | 602 | 2.71e-97 | 313 |
| MsG0880046919.01.T01 | AT3G16030 | 53.767 | 292 | 115 | 4 | 350 | 637 | 508 | 783 | 1.57e-96 | 315 |
| MsG0880046919.01.T01 | AT3G16030 | 53.767 | 292 | 115 | 4 | 350 | 637 | 529 | 804 | 5.16e-96 | 315 |
| MsG0880046919.01.T01 | AT4G23200 | 53.135 | 303 | 120 | 4 | 341 | 637 | 305 | 591 | 9.01e-95 | 306 |
| MsG0880046919.01.T01 | AT4G11480 | 48.408 | 314 | 141 | 4 | 331 | 641 | 287 | 582 | 2.13e-94 | 305 |
| MsG0880046919.01.T01 | AT1G67520 | 52.397 | 292 | 119 | 4 | 350 | 637 | 240 | 515 | 2.16e-94 | 303 |
| MsG0880046919.01.T01 | AT4G05200 | 51.155 | 303 | 127 | 4 | 340 | 637 | 315 | 601 | 3.60e-94 | 305 |
| MsG0880046919.01.T01 | AT4G05200 | 51.155 | 303 | 127 | 4 | 340 | 637 | 333 | 619 | 5.40e-94 | 305 |
| MsG0880046919.01.T01 | AT4G23240 | 51.163 | 301 | 125 | 5 | 340 | 637 | 2 | 283 | 5.54e-94 | 294 |
Find 125 sgRNAs with CRISPR-Local
Find 583 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACTATAAACGTCATACT+TGG | 0.415820 | 8:+79836840 | None:intergenic |
| AAAAGAAAATAAGCTTGGAG+AGG | 0.615736 | 8:-79838469 | MsG0880046919.01.T01:CDS |
| AAAAGTTATATTAGAAGCTT+TGG | 0.315296 | 8:-79839197 | MsG0880046919.01.T01:CDS |
| AAACAAGGTGAAGGTGGTGT+TGG | 0.521933 | 8:-79839275 | MsG0880046919.01.T01:intron |
| AAACTATAAACGTCATACTT+GGG | 0.577980 | 8:+79836841 | None:intergenic |
| AAAGAAAATAAGCTTGGAGA+GGG | 0.594999 | 8:-79838468 | MsG0880046919.01.T01:CDS |
| AACAAAAGAAAGAAAGATGT+TGG | 0.455643 | 8:-79846715 | MsG0880046919.01.T01:exon |
| AACAACTTCATTAGCTGACT+TGG | 0.469974 | 8:+79845298 | None:intergenic |
| AACTTGAAGCAAACACAAGC+AGG | 0.551523 | 8:-79836996 | MsG0880046919.01.T01:CDS |
| AAGAAATCATTGTACCTTGA+GGG | 0.480509 | 8:+79844685 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAGAAAATAAGCTTGGAG+AGG | - | Chr8:79844741-79844760 | MsG0880046919.01.T01:CDS | 30.0% | |
| AAAGAAAATAAGCTTGGAGA+GGG | - | Chr8:79844742-79844761 | MsG0880046919.01.T01:CDS | 30.0% | |
| AAATCATTGCTTGTTTGGTA+TGG | - | Chr8:79843031-79843050 | MsG0880046919.01.T01:intron | 30.0% | |
| AACACAACTTCTAGATATAC+AGG | + | Chr8:79836831-79836850 | None:intergenic | 30.0% | |
| AAGAAAGGGAAAAAAATGCA+AGG | + | Chr8:79842472-79842491 | None:intergenic | 30.0% | |
| AAGAAATCATTGTACCTTGA+GGG | + | Chr8:79838528-79838547 | None:intergenic | 30.0% | |
| AAGCAAACATATGTGATTGT+TGG | - | Chr8:79840784-79840803 | MsG0880046919.01.T01:intron | 30.0% | |
| AAGTATTACTCCCAAGATAA+TGG | + | Chr8:79844440-79844459 | None:intergenic | 30.0% | |
| AATATTGTTTGTCAGTAGCA+TGG | - | Chr8:79839194-79839213 | MsG0880046919.01.T01:CDS | 30.0% | |
| AATCATTGCTTGTTTGGTAT+GGG | - | Chr8:79843032-79843051 | MsG0880046919.01.T01:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 79836446 | 79846786 | 79836446 | ID=MsG0880046919.01;Name=MsG0880046919.01 |
| Chr8 | mRNA | 79836446 | 79846786 | 79836446 | ID=MsG0880046919.01.T01;Parent=MsG0880046919.01;Name=MsG0880046919.01.T01;_AED=0.37;_eAED=0.42;_QI=65|0|0.16|1|1|1|12|0|648 |
| Chr8 | exon | 79836446 | 79836592 | 79836446 | ID=MsG0880046919.01.T01:exon:10161;Parent=MsG0880046919.01.T01 |
| Chr8 | exon | 79836759 | 79836906 | 79836759 | ID=MsG0880046919.01.T01:exon:10160;Parent=MsG0880046919.01.T01 |
| Chr8 | exon | 79836982 | 79837219 | 79836982 | ID=MsG0880046919.01.T01:exon:10159;Parent=MsG0880046919.01.T01 |
| Chr8 | exon | 79837331 | 79837589 | 79837331 | ID=MsG0880046919.01.T01:exon:10158;Parent=MsG0880046919.01.T01 |
| Chr8 | exon | 79838446 | 79838662 | 79838446 | ID=MsG0880046919.01.T01:exon:10157;Parent=MsG0880046919.01.T01 |
| Chr8 | exon | 79838757 | 79838837 | 79838757 | ID=MsG0880046919.01.T01:exon:10156;Parent=MsG0880046919.01.T01 |
| Chr8 | exon | 79839181 | 79839290 | 79839181 | ID=MsG0880046919.01.T01:exon:10155;Parent=MsG0880046919.01.T01 |
| Chr8 | exon | 79842830 | 79842939 | 79842830 | ID=MsG0880046919.01.T01:exon:10154;Parent=MsG0880046919.01.T01 |
| Chr8 | exon | 79844670 | 79844839 | 79844670 | ID=MsG0880046919.01.T01:exon:10153;Parent=MsG0880046919.01.T01 |
| Chr8 | exon | 79845167 | 79845361 | 79845167 | ID=MsG0880046919.01.T01:exon:10152;Parent=MsG0880046919.01.T01 |
| Chr8 | exon | 79846289 | 79846355 | 79846289 | ID=MsG0880046919.01.T01:exon:10151;Parent=MsG0880046919.01.T01 |
| Chr8 | exon | 79846517 | 79846786 | 79846517 | ID=MsG0880046919.01.T01:exon:10150;Parent=MsG0880046919.01.T01 |
| Chr8 | five_prime_UTR | 79846722 | 79846786 | 79846722 | ID=MsG0880046919.01.T01:five_prime_utr;Parent=MsG0880046919.01.T01 |
| Chr8 | CDS | 79846517 | 79846721 | 79846517 | ID=MsG0880046919.01.T01:cds;Parent=MsG0880046919.01.T01 |
| Chr8 | CDS | 79846289 | 79846355 | 79846289 | ID=MsG0880046919.01.T01:cds;Parent=MsG0880046919.01.T01 |
| Chr8 | CDS | 79845167 | 79845361 | 79845167 | ID=MsG0880046919.01.T01:cds;Parent=MsG0880046919.01.T01 |
| Chr8 | CDS | 79844670 | 79844839 | 79844670 | ID=MsG0880046919.01.T01:cds;Parent=MsG0880046919.01.T01 |
| Chr8 | CDS | 79842830 | 79842939 | 79842830 | ID=MsG0880046919.01.T01:cds;Parent=MsG0880046919.01.T01 |
| Chr8 | CDS | 79839181 | 79839290 | 79839181 | ID=MsG0880046919.01.T01:cds;Parent=MsG0880046919.01.T01 |
| Chr8 | CDS | 79838757 | 79838837 | 79838757 | ID=MsG0880046919.01.T01:cds;Parent=MsG0880046919.01.T01 |
| Chr8 | CDS | 79838446 | 79838662 | 79838446 | ID=MsG0880046919.01.T01:cds;Parent=MsG0880046919.01.T01 |
| Chr8 | CDS | 79837331 | 79837589 | 79837331 | ID=MsG0880046919.01.T01:cds;Parent=MsG0880046919.01.T01 |
| Chr8 | CDS | 79836982 | 79837219 | 79836982 | ID=MsG0880046919.01.T01:cds;Parent=MsG0880046919.01.T01 |
| Chr8 | CDS | 79836759 | 79836906 | 79836759 | ID=MsG0880046919.01.T01:cds;Parent=MsG0880046919.01.T01 |
| Chr8 | CDS | 79836446 | 79836592 | 79836446 | ID=MsG0880046919.01.T01:cds;Parent=MsG0880046919.01.T01 |
| Gene Sequence |
>MsG0880046919.01.T01 ATGTTGGGTGTGTTCAGCAGCTCCGTCGTGTCCCCGCCAGATGAGCTCGTTGCAGCTGGCAGCCGAACGCCGTCACCTAAGACGACTGCGACGGCACTGTTGAAGCGTTTCTCTGAAAGCAACGGTGGTTCAACAGTGTCAGTGGAAGTTGGAGATCAAGTCCGGTTGCCTATACCCATCAGAACGAGTCGTCGTTACAACCCAGATCCTGTTAAGAATCTGGCTTATGTGGCCAATTTTACATTCATCTTCATCTTTTTCAAAGTGAAAAACTCTAGACATTTAGGGAGATTAAGGCAACAATATGGACTAGCCAAGTCAGCTAATGAAGTTGTTTTGGTGATTGAGGCCTACAAAGCTTTACGTGACAGAGCACCTTACCCTCCCAATCACGTTGTTGGTCATCTCAGTGGCACTTTTGCTTTCATACTTTTTGACAAATCACTTCTACCCTTTTTGTGGCCTCTAGGGAGTATTTTTTTGACAGGATCATTTGGTAAGGTACCACTATTTTGGGGAATAACTGCTGATGGATATGTAGCATTTGCTGATGATGCTGAATTGCTTAAAGGTGCTTGTGGCAAGTCACTTGCTTCTTTCCCTCAAGGTACAATGATTTCTTCTATCATGGAAATTAGATGTTTCTATTCAACTGCTGTTGGAGGATTGATGTGCTATGAGAACCCTAAGAATAAAATTACTGCAGTGCCTGCTAATGAAGAGGATTTCTGGGGTGCAACCTTCAAGGTGAAGGTGGTGTTGGAATACCCTTCTGGTCCTGCTATTACAATGGATGTTCAATGGCTTAAAGTTGATAAAAAAGTTATATTAGAAGCTTTGGTGATGAGTAGTATCATTTCAGCTCAAGAATTAGATGCTGCTCACTACTCTGGCAGCTGCCATTATCTTGGGAGTAATACTTTGTTACTCCCACCAAGTTTTTATTTATCTCAAACTCCTTTGTTAGGAACAATTACCACTGCATTTACAAGAATGATATACAAAATGAGGATATATTTATCAGCAGCTGAAGAAGATCTCAATGGAAATGGTTCTGATTTAATAGTATTTAATTATCTCTCAATTAAAGTGGCAACAGATAACTTCTCAAAAGAAAATAAGCTTGGAGAGGGTGGATTTGGTGCAGTGTACAAGGGAAAATTACGGAAGGGACAGGAAATAGCTGTCAAAAGACTATCAGAAACCTCCAATCAAGGACTCGAAGAGTTCAAAAATGAGATCACACTTACAGCAAGACTACAACATGTGAATTTAGTTAGACTTTTGGGTTACTGCACAAAAAGGAATGAGAAGCTTCTGATCTATGAATACCTCCCAAATAAAAGCTTAGATCATTCTCTCTTTGGTCTGTTACTAATATTTTATTTGCTACTAAGCTCAATTAATGTTCAAAATCCAAGGAAATCAATTCTTCTAGATTGGCGTAAGCGTGTGCATATCATTGAAGGAATTACTCAAGGCCTTCTTTATCTCCAAGAGTACTCAAATTTTACTATAATCCACCGAGATCTTAAAGCTAGTAATGTTTTGTTAGACCATGATATGAATCCTAAGATATCAGATTTTGGTATGGCTAGAATTTTCGGGAAGTATGAACTTGAAGCAAACACAAGCAGGATCGTGGGAACATATGGGTATGTACCTCCAGAGTATGTCAGAAAAGGCATATACTCACCCAAGTATGACGTTTATAGTTTTGGAGTTCTTCTCTTGCAAATCATAAGTGGAAAGACTTCGCATTATTACGGCACACATGAAAATATGAACCTTTTGGAATACGCTTATGAGCTCTGGATAGAAGGGAGAGGCATGGAGTTTTTTGACCCTTCATTGGATGATTCGACCTCACATTGTAAAATCATGAGATGCATGCAGATAGCTCTTTGTGTGTTCAAGAGAACTCAGCAGACAGACCTTTCATGTTAG |
| Protein sequence |
>MsG0880046919.01.T01 MLGVFSSSVVSPPDELVAAGSRTPSPKTTATALLKRFSESNGGSTVSVEVGDQVRLPIPIRTSRRYNPDPVKNLAYVANFTFIFIFFKVKNSRHLGRLRQQYGLAKSANEVVLVIEAYKALRDRAPYPPNHVVGHLSGTFAFILFDKSLLPFLWPLGSIFLTGSFGKVPLFWGITADGYVAFADDAELLKGACGKSLASFPQGTMISSIMEIRCFYSTAVGGLMCYENPKNKITAVPANEEDFWGATFKVKVVLEYPSGPAITMDVQWLKVDKKVILEALVMSSIISAQELDAAHYSGSCHYLGSNTLLLPPSFYLSQTPLLGTITTAFTRMIYKMRIYLSAAEEDLNGNGSDLIVFNYLSIKVATDNFSKENKLGEGGFGAVYKGKLRKGQEIAVKRLSETSNQGLEEFKNEITLTARLQHVNLVRLLGYCTKRNEKLLIYEYLPNKSLDHSLFGLLLIFYLLLSSINVQNPRKSILLDWRKRVHIIEGITQGLLYLQEYSNFTIIHRDLKASNVLLDHDMNPKISDFGMARIFGKYELEANTSRIVGTYGYVPPEYVRKGIYSPKYDVYSFGVLLLQIISGKTSHYYGTHENMNLLEYAYELWIEGRGMEFFDPSLDDSTSHCKIMRCMQIALCVFKRTQQTDLSC* |
