Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047000.01.T01 | AES91783.1 | 90.055 | 181 | 18 | 0 | 1 | 181 | 1 | 181 | 8.57E-111 | 323 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047000.01.T01 | G7JJ33 | 90.055 | 181 | 18 | 0 | 1 | 181 | 1 | 181 | 4.09e-111 | 323 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0880047000.01.T01 | TF | bHLH |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047000.01.T01 | MTR_4g118830 | 90.055 | 181 | 18 | 0 | 1 | 181 | 1 | 181 | 1.04e-114 | 323 |
| MsG0880047000.01.T01 | MTR_4g118430 | 45.045 | 111 | 42 | 3 | 77 | 181 | 1 | 98 | 3.78e-20 | 80.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 34 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTTCTCTGTTACGGTTAC+TGG | 0.183266 | 8:+80790029 | MsG0880047000.01.T01:CDS |
| AGTAACCGTAACAGAGAAAT+TGG | 0.195649 | 8:-80790027 | None:intergenic |
| TCAAGCAATGTGGCTTTCTT+TGG | 0.199938 | 8:+80790053 | MsG0880047000.01.T01:CDS |
| GAGAGAGATTATTAGTTTGT+AGG | 0.261530 | 8:+80790238 | MsG0880047000.01.T01:CDS |
| TGTTGGTTCCGTGTCGTAAT+AGG | 0.277898 | 8:+80790000 | MsG0880047000.01.T01:CDS |
| CAAGCAATGTGGCTTTCTTT+GGG | 0.307847 | 8:+80790054 | MsG0880047000.01.T01:CDS |
| TAACAAATCAGTTTCTGAAA+TGG | 0.363545 | 8:+80789591 | MsG0880047000.01.T01:CDS |
| GATCTTGGCGATCACTGCTT+TGG | 0.383378 | 8:+80790184 | MsG0880047000.01.T01:CDS |
| AACAAATCAGTTTCTGAAAT+GGG | 0.410347 | 8:+80789592 | MsG0880047000.01.T01:CDS |
| TTACATTGGGGTAGAGAGTT+GGG | 0.421719 | 8:-80789720 | None:intergenic |
| GAATCCCAATTTCTCTGTTA+CGG | 0.433893 | 8:+80790022 | MsG0880047000.01.T01:CDS |
| TAATGATGGTAATTTGATCT+TGG | 0.443216 | 8:+80790169 | MsG0880047000.01.T01:CDS |
| GAAAATGTTGGAGGAGATGA+AGG | 0.445802 | 8:+80789938 | MsG0880047000.01.T01:CDS |
| GTTACATTGGGGTAGAGAGT+TGG | 0.468914 | 8:-80789721 | None:intergenic |
| ATCAAAGAAACCTGTTGAAG+GGG | 0.470795 | 8:+80789968 | MsG0880047000.01.T01:CDS |
| TGAAGAAAAGAAGAAAATGT+TGG | 0.489430 | 8:+80789926 | MsG0880047000.01.T01:CDS |
| GCTAATGTTTCTGTTAATGA+TGG | 0.496957 | 8:+80790155 | MsG0880047000.01.T01:CDS |
| TTTCTTTGGGATTGAATCTG+TGG | 0.501659 | 8:+80790067 | MsG0880047000.01.T01:CDS |
| GAGTTGGGACAGTGGTAGTG+AGG | 0.504434 | 8:-80789705 | None:intergenic |
| ATTGAATCTGTGGCTAAACC+TGG | 0.515198 | 8:+80790077 | MsG0880047000.01.T01:CDS |
| TTCAACGTTTAGAACCTCTT+CGG | 0.524218 | 8:-80789644 | None:intergenic |
| AAATCAAAGAAACCTGTTGA+AGG | 0.526426 | 8:+80789966 | MsG0880047000.01.T01:CDS |
| AGAAAAGAAGAAAATGTTGG+AGG | 0.526690 | 8:+80789929 | MsG0880047000.01.T01:CDS |
| AATCAAAGAAACCTGTTGAA+GGG | 0.548949 | 8:+80789967 | MsG0880047000.01.T01:CDS |
| GTAACCGTAACAGAGAAATT+GGG | 0.551700 | 8:-80790026 | None:intergenic |
| TAAACGTTGAACGTGAAAGA+AGG | 0.567677 | 8:+80789656 | MsG0880047000.01.T01:CDS |
| TCAAAGAAACCTGTTGAAGG+GGG | 0.622647 | 8:+80789969 | MsG0880047000.01.T01:CDS |
| CACTACTACTAAAACCGAAG+AGG | 0.646167 | 8:+80789630 | MsG0880047000.01.T01:CDS |
| AAAATCACAGTAATCAAACC+AGG | 0.647796 | 8:-80790095 | None:intergenic |
| GGTTACTGGTTCAAGCAATG+TGG | 0.661828 | 8:+80790043 | MsG0880047000.01.T01:CDS |
| GGGGTAGAGAGTTGGGACAG+TGG | 0.661869 | 8:-80789713 | None:intergenic |
| TACACAGGAAGTGATTGTGA+AGG | 0.673700 | 8:+80789878 | MsG0880047000.01.T01:CDS |
| TTGGGATTCCTATTACGACA+CGG | 0.678110 | 8:-80790008 | None:intergenic |
| GAAATGGGTAACAGAAAACG+TGG | 0.703774 | 8:+80789607 | MsG0880047000.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGAAAATAAAAGAGAAAA+TGG | - | Chr8:80789761-80789780 | None:intergenic | 15.0% |
| !! | ATAACAAATCAAACTATAAT+GGG | - | Chr8:80789833-80789852 | None:intergenic | 15.0% |
| !!! | TTTATTTTCATTCTTCATTT+GGG | + | Chr8:80789769-80789788 | MsG0880047000.01.T01:intron | 15.0% |
| !!! | TTTTATTTTCATTCTTCATT+TGG | + | Chr8:80789768-80789787 | MsG0880047000.01.T01:intron | 15.0% |
| !! | AAAATAAAAGAGAAAATGGA+AGG | - | Chr8:80789757-80789776 | None:intergenic | 20.0% |
| !! | GATAACAAATCAAACTATAA+TGG | - | Chr8:80789834-80789853 | None:intergenic | 20.0% |
| !! | TGCATTTAAACATGAAAAAT+TGG | - | Chr8:80789798-80789817 | None:intergenic | 20.0% |
| !!! | TTTGATTACTGTGATTTTTA+AGG | + | Chr8:80790100-80790119 | MsG0880047000.01.T01:CDS | 20.0% |
| !!! | TTTGATTTGTTATCTGAAAT+GGG | + | Chr8:80789840-80789859 | MsG0880047000.01.T01:intron | 20.0% |
| ! | TAATGATGGTAATTTGATCT+TGG | + | Chr8:80790169-80790188 | MsG0880047000.01.T01:CDS | 25.0% |
| ! | TGAAGAAAAGAAGAAAATGT+TGG | + | Chr8:80789926-80789945 | MsG0880047000.01.T01:CDS | 25.0% |
| !! | TAACAAATCAGTTTCTGAAA+TGG | + | Chr8:80789591-80789610 | MsG0880047000.01.T01:CDS | 25.0% |
| !!! | AACAAATCAGTTTCTGAAAT+GGG | + | Chr8:80789592-80789611 | MsG0880047000.01.T01:CDS | 25.0% |
| !!! | GTTTGATTTGTTATCTGAAA+TGG | + | Chr8:80789839-80789858 | MsG0880047000.01.T01:intron | 25.0% |
| !!! | TGAAATGGGTTTTTGTTTTT+AGG | + | Chr8:80789854-80789873 | MsG0880047000.01.T01:intron | 25.0% |
| AAAATCACAGTAATCAAACC+AGG | - | Chr8:80790098-80790117 | None:intergenic | 30.0% | |
| AAATCAAAGAAACCTGTTGA+AGG | + | Chr8:80789966-80789985 | MsG0880047000.01.T01:CDS | 30.0% | |
| AATCAAAGAAACCTGTTGAA+GGG | + | Chr8:80789967-80789986 | MsG0880047000.01.T01:CDS | 30.0% | |
| AGAAAAGAAGAAAATGTTGG+AGG | + | Chr8:80789929-80789948 | MsG0880047000.01.T01:CDS | 30.0% | |
| AGGAATGATGTTAGTTACAT+TGG | - | Chr8:80789737-80789756 | None:intergenic | 30.0% | |
| GAATGATGTTAGTTACATTG+GGG | - | Chr8:80789735-80789754 | None:intergenic | 30.0% | |
| GGAATGATGTTAGTTACATT+GGG | - | Chr8:80789736-80789755 | None:intergenic | 30.0% | |
| ! | GCTAATGTTTCTGTTAATGA+TGG | + | Chr8:80790155-80790174 | MsG0880047000.01.T01:CDS | 30.0% |
| !!! | TTTTTGTTTTTAGGCTACAC+AGG | + | Chr8:80789863-80789882 | MsG0880047000.01.T01:intron | 30.0% |
| AAATTAAAACCCCCTTCAAC+AGG | - | Chr8:80789981-80790000 | None:intergenic | 35.0% | |
| AATTTCTCTGTTACGGTTAC+TGG | + | Chr8:80790029-80790048 | MsG0880047000.01.T01:CDS | 35.0% | |
| AGTAACCGTAACAGAGAAAT+TGG | - | Chr8:80790030-80790049 | None:intergenic | 35.0% | |
| ATCAAAGAAACCTGTTGAAG+GGG | + | Chr8:80789968-80789987 | MsG0880047000.01.T01:CDS | 35.0% | |
| GAATCCCAATTTCTCTGTTA+CGG | + | Chr8:80790022-80790041 | MsG0880047000.01.T01:CDS | 35.0% | |
| GTAACCGTAACAGAGAAATT+GGG | - | Chr8:80790029-80790048 | None:intergenic | 35.0% | |
| TAAACGTTGAACGTGAAAGA+AGG | + | Chr8:80789656-80789675 | MsG0880047000.01.T01:CDS | 35.0% | |
| ! | TTCAACGTTTAGAACCTCTT+CGG | - | Chr8:80789647-80789666 | None:intergenic | 35.0% |
| !! | GTTTTTGAAGAATGAAGCTG+AGG | + | Chr8:80790124-80790143 | MsG0880047000.01.T01:CDS | 35.0% |
| !! | TTTCTTTGGGATTGAATCTG+TGG | + | Chr8:80790067-80790086 | MsG0880047000.01.T01:CDS | 35.0% |
| !!! | TGAAGGGGGTTTTAATTTGT+TGG | + | Chr8:80789983-80790002 | MsG0880047000.01.T01:CDS | 35.0% |
| ATTGAATCTGTGGCTAAACC+TGG | + | Chr8:80790077-80790096 | MsG0880047000.01.T01:CDS | 40.0% | |
| CAAGCAATGTGGCTTTCTTT+GGG | + | Chr8:80790054-80790073 | MsG0880047000.01.T01:CDS | 40.0% | |
| CACTACTACTAAAACCGAAG+AGG | + | Chr8:80789630-80789649 | MsG0880047000.01.T01:CDS | 40.0% | |
| GAAAATGTTGGAGGAGATGA+AGG | + | Chr8:80789938-80789957 | MsG0880047000.01.T01:CDS | 40.0% | |
| GAAATGGGTAACAGAAAACG+TGG | + | Chr8:80789607-80789626 | MsG0880047000.01.T01:CDS | 40.0% | |
| TACACAGGAAGTGATTGTGA+AGG | + | Chr8:80789878-80789897 | MsG0880047000.01.T01:CDS | 40.0% | |
| TCAAAGAAACCTGTTGAAGG+GGG | + | Chr8:80789969-80789988 | MsG0880047000.01.T01:CDS | 40.0% | |
| TCAAGCAATGTGGCTTTCTT+TGG | + | Chr8:80790053-80790072 | MsG0880047000.01.T01:CDS | 40.0% | |
| TTACATTGGGGTAGAGAGTT+GGG | - | Chr8:80789723-80789742 | None:intergenic | 40.0% | |
| TTGGGATTCCTATTACGACA+CGG | - | Chr8:80790011-80790030 | None:intergenic | 40.0% | |
| GTTACATTGGGGTAGAGAGT+TGG | - | Chr8:80789724-80789743 | None:intergenic | 45.0% | |
| !! | GGTTACTGGTTCAAGCAATG+TGG | + | Chr8:80790043-80790062 | MsG0880047000.01.T01:CDS | 45.0% |
| !! | TGTTGGTTCCGTGTCGTAAT+AGG | + | Chr8:80790000-80790019 | MsG0880047000.01.T01:CDS | 45.0% |
| GATCTTGGCGATCACTGCTT+TGG | + | Chr8:80790184-80790203 | MsG0880047000.01.T01:CDS | 50.0% | |
| ! | GAGTTGGGACAGTGGTAGTG+AGG | - | Chr8:80789708-80789727 | None:intergenic | 55.0% |
| GGGGTAGAGAGTTGGGACAG+TGG | - | Chr8:80789716-80789735 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 80789577 | 80790259 | 80789577 | ID=MsG0880047000.01;Name=MsG0880047000.01 |
| Chr8 | mRNA | 80789577 | 80790259 | 80789577 | ID=MsG0880047000.01.T01;Parent=MsG0880047000.01;Name=MsG0880047000.01.T01;_AED=0.42;_eAED=0.43;_QI=0|0|0|1|1|1|2|0|181 |
| Chr8 | exon | 80789577 | 80789738 | 80789577 | ID=MsG0880047000.01.T01:exon:10446;Parent=MsG0880047000.01.T01 |
| Chr8 | exon | 80789876 | 80790259 | 80789876 | ID=MsG0880047000.01.T01:exon:10447;Parent=MsG0880047000.01.T01 |
| Chr8 | CDS | 80789577 | 80789738 | 80789577 | ID=MsG0880047000.01.T01:cds;Parent=MsG0880047000.01.T01 |
| Chr8 | CDS | 80789876 | 80790259 | 80789876 | ID=MsG0880047000.01.T01:cds;Parent=MsG0880047000.01.T01 |
| Gene Sequence |
| Protein sequence |
