Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047027.01.T01 | AES91843.2 | 97.674 | 129 | 3 | 0 | 1 | 129 | 1 | 129 | 5.42E-89 | 264 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047027.01.T01 | O82248 | 77.551 | 98 | 22 | 0 | 19 | 116 | 48 | 145 | 1.05E-54 | 171 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047027.01.T01 | G7JKC3 | 97.674 | 129 | 3 | 0 | 1 | 129 | 1 | 129 | 2.59e-89 | 264 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0880047027.01.T01 | TF | NF-YB |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047027.01.T01 | MTR_4g119500 | 97.674 | 129 | 3 | 0 | 1 | 129 | 1 | 129 | 6.56e-93 | 264 |
| MsG0880047027.01.T01 | MTR_0392s0020 | 86.614 | 127 | 12 | 4 | 2 | 128 | 1 | 122 | 1.37e-71 | 211 |
| MsG0880047027.01.T01 | MTR_1g088860 | 74.107 | 112 | 29 | 0 | 5 | 116 | 19 | 130 | 4.58e-61 | 186 |
| MsG0880047027.01.T01 | MTR_4g133938 | 67.273 | 110 | 31 | 1 | 12 | 116 | 4 | 113 | 7.39e-51 | 158 |
| MsG0880047027.01.T01 | MTR_1g072790 | 66.990 | 103 | 34 | 0 | 21 | 123 | 28 | 130 | 6.97e-50 | 157 |
| MsG0880047027.01.T01 | MTR_2g026710 | 65.138 | 109 | 35 | 1 | 12 | 117 | 4 | 112 | 2.89e-49 | 155 |
| MsG0880047027.01.T01 | MTR_7g061270 | 62.162 | 111 | 40 | 1 | 7 | 115 | 9 | 119 | 1.08e-48 | 154 |
| MsG0880047027.01.T01 | MTR_7g100650 | 62.162 | 111 | 40 | 1 | 7 | 115 | 9 | 119 | 1.75e-48 | 153 |
| MsG0880047027.01.T01 | MTR_8g091720 | 62.385 | 109 | 41 | 0 | 21 | 129 | 27 | 135 | 4.20e-48 | 153 |
| MsG0880047027.01.T01 | MTR_3g058980 | 67.010 | 97 | 32 | 0 | 21 | 117 | 26 | 122 | 5.71e-48 | 152 |
| MsG0880047027.01.T01 | MTR_5g095740 | 67.010 | 97 | 32 | 0 | 21 | 117 | 22 | 118 | 1.13e-47 | 151 |
| MsG0880047027.01.T01 | MTR_1g083070 | 72.632 | 95 | 26 | 0 | 22 | 116 | 4 | 98 | 1.60e-47 | 149 |
| MsG0880047027.01.T01 | MTR_8g093920 | 69.892 | 93 | 28 | 0 | 21 | 113 | 39 | 131 | 4.04e-47 | 151 |
| MsG0880047027.01.T01 | MTR_4g133952 | 60.000 | 110 | 44 | 0 | 19 | 128 | 55 | 164 | 5.17e-47 | 152 |
| MsG0880047027.01.T01 | MTR_1g039040 | 63.830 | 94 | 34 | 0 | 20 | 113 | 4 | 97 | 3.55e-42 | 138 |
| MsG0880047027.01.T01 | MTR_1g029070 | 47.872 | 94 | 49 | 0 | 21 | 114 | 51 | 144 | 2.14e-26 | 97.4 |
| MsG0880047027.01.T01 | MTR_5g095900 | 46.237 | 93 | 50 | 0 | 19 | 111 | 13 | 105 | 5.11e-25 | 94.0 |
| MsG0880047027.01.T01 | MTR_1g028480 | 44.304 | 79 | 39 | 1 | 47 | 125 | 14 | 87 | 1.06e-18 | 75.9 |
| MsG0880047027.01.T01 | MTR_1g029100 | 36.250 | 80 | 51 | 0 | 27 | 106 | 7 | 86 | 5.89e-17 | 70.9 |
| MsG0880047027.01.T01 | MTR_2g056000 | 32.955 | 88 | 59 | 0 | 23 | 110 | 42 | 129 | 6.84e-16 | 70.5 |
| MsG0880047027.01.T01 | MTR_4g112380 | 30.682 | 88 | 61 | 0 | 23 | 110 | 12 | 99 | 4.52e-15 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047027.01.T01 | AT2G47810 | 77.551 | 98 | 22 | 0 | 19 | 116 | 48 | 145 | 1.07e-55 | 171 |
| MsG0880047027.01.T01 | AT2G38880 | 69.307 | 101 | 31 | 0 | 15 | 115 | 14 | 114 | 3.58e-51 | 159 |
| MsG0880047027.01.T01 | AT2G38880 | 69.307 | 101 | 31 | 0 | 15 | 115 | 14 | 114 | 4.90e-51 | 159 |
| MsG0880047027.01.T01 | AT2G38880 | 69.307 | 101 | 31 | 0 | 15 | 115 | 14 | 114 | 4.90e-51 | 159 |
| MsG0880047027.01.T01 | AT2G38880 | 69.307 | 101 | 31 | 0 | 15 | 115 | 14 | 114 | 4.90e-51 | 159 |
| MsG0880047027.01.T01 | AT4G14540 | 60.000 | 125 | 46 | 2 | 3 | 127 | 5 | 125 | 1.38e-50 | 158 |
| MsG0880047027.01.T01 | AT1G09030 | 71.429 | 98 | 28 | 0 | 20 | 117 | 1 | 98 | 7.43e-50 | 155 |
| MsG0880047027.01.T01 | AT5G47640 | 66.667 | 108 | 32 | 1 | 21 | 124 | 26 | 133 | 1.33e-49 | 157 |
| MsG0880047027.01.T01 | AT2G38880 | 68.367 | 98 | 31 | 0 | 15 | 112 | 14 | 111 | 1.89e-49 | 154 |
| MsG0880047027.01.T01 | AT2G38880 | 69.072 | 97 | 30 | 0 | 15 | 111 | 14 | 110 | 3.47e-49 | 154 |
| MsG0880047027.01.T01 | AT2G38880 | 68.367 | 98 | 31 | 0 | 15 | 112 | 14 | 111 | 6.81e-49 | 154 |
| MsG0880047027.01.T01 | AT2G38880 | 68.367 | 98 | 31 | 0 | 15 | 112 | 14 | 111 | 6.81e-49 | 154 |
| MsG0880047027.01.T01 | AT3G53340 | 66.337 | 101 | 34 | 0 | 20 | 120 | 27 | 127 | 7.81e-49 | 153 |
| MsG0880047027.01.T01 | AT3G53340 | 66.337 | 101 | 34 | 0 | 20 | 120 | 27 | 127 | 7.81e-49 | 153 |
| MsG0880047027.01.T01 | AT2G38880 | 68.367 | 98 | 31 | 0 | 15 | 112 | 14 | 111 | 8.30e-49 | 153 |
| MsG0880047027.01.T01 | AT2G38880 | 68.367 | 98 | 31 | 0 | 15 | 112 | 14 | 111 | 8.30e-49 | 153 |
| MsG0880047027.01.T01 | AT2G38880 | 68.367 | 98 | 31 | 0 | 15 | 112 | 14 | 111 | 8.30e-49 | 153 |
| MsG0880047027.01.T01 | AT3G53340 | 68.750 | 96 | 30 | 0 | 20 | 115 | 27 | 122 | 2.11e-48 | 153 |
| MsG0880047027.01.T01 | AT3G53340 | 68.750 | 96 | 30 | 0 | 20 | 115 | 27 | 122 | 2.11e-48 | 153 |
| MsG0880047027.01.T01 | AT2G37060 | 61.682 | 107 | 41 | 0 | 20 | 126 | 28 | 134 | 7.97e-48 | 152 |
| MsG0880047027.01.T01 | AT2G37060 | 61.682 | 107 | 41 | 0 | 20 | 126 | 28 | 134 | 7.97e-48 | 152 |
| MsG0880047027.01.T01 | AT2G37060 | 61.682 | 107 | 41 | 0 | 20 | 126 | 28 | 134 | 7.97e-48 | 152 |
| MsG0880047027.01.T01 | AT3G53340 | 68.817 | 93 | 29 | 0 | 20 | 112 | 27 | 119 | 2.91e-47 | 149 |
| MsG0880047027.01.T01 | AT2G13570 | 59.829 | 117 | 47 | 0 | 1 | 117 | 15 | 131 | 4.75e-47 | 151 |
| MsG0880047027.01.T01 | AT5G47670 | 61.905 | 105 | 36 | 1 | 16 | 116 | 19 | 123 | 6.85e-45 | 145 |
| MsG0880047027.01.T01 | AT5G47670 | 61.905 | 105 | 36 | 1 | 16 | 116 | 48 | 152 | 1.39e-44 | 145 |
| MsG0880047027.01.T01 | AT5G47670 | 61.905 | 105 | 36 | 1 | 16 | 116 | 48 | 152 | 1.39e-44 | 145 |
| MsG0880047027.01.T01 | AT1G21970 | 52.632 | 114 | 45 | 1 | 12 | 116 | 40 | 153 | 1.41e-40 | 135 |
| MsG0880047027.01.T01 | AT5G08190 | 30.612 | 98 | 66 | 1 | 22 | 117 | 11 | 108 | 1.24e-15 | 69.3 |
| MsG0880047027.01.T01 | AT5G08190 | 30.851 | 94 | 63 | 1 | 22 | 113 | 11 | 104 | 4.09e-15 | 68.2 |
| MsG0880047027.01.T01 | AT5G23090 | 30.928 | 97 | 65 | 1 | 23 | 117 | 12 | 108 | 4.48e-15 | 67.8 |
| MsG0880047027.01.T01 | AT5G23090 | 31.183 | 93 | 62 | 1 | 23 | 113 | 12 | 104 | 2.88e-14 | 65.9 |
| MsG0880047027.01.T01 | AT5G23090 | 31.183 | 93 | 62 | 1 | 23 | 113 | 12 | 104 | 2.88e-14 | 65.9 |
| MsG0880047027.01.T01 | AT5G23090 | 31.183 | 93 | 62 | 1 | 23 | 113 | 12 | 104 | 2.88e-14 | 65.9 |
Find 27 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGATATCTGTTGGGCTTT+GGG | 0.254012 | 8:-81162922 | MsG0880047027.01.T01:CDS |
| TGATGATATCTGTTGGGCTT+TGG | 0.268785 | 8:-81162923 | MsG0880047027.01.T01:CDS |
| AGAAATTAATCTCCTCTATC+TGG | 0.292960 | 8:+81162799 | None:intergenic |
| GCATAGATATAGAGAATTAG+AGG | 0.332962 | 8:-81162854 | MsG0880047027.01.T01:CDS |
| ATCCTCCCAACATTTGCTAT+TGG | 0.373158 | 8:+81163090 | None:intergenic |
| TGTTGGGCTTTGGGGACACT+TGG | 0.405725 | 8:-81162913 | MsG0880047027.01.T01:CDS |
| GGAATATTATTAGCAGTAGT+TGG | 0.426129 | 8:+81162820 | None:intergenic |
| CTATGCAAATACCTTCTCAT+TGG | 0.433135 | 8:+81162871 | None:intergenic |
| GCTAATAATATTCCAGATAG+AGG | 0.450061 | 8:-81162811 | MsG0880047027.01.T01:CDS |
| TGAAAACGGAGGCATAATAA+AGG | 0.463124 | 8:-81163130 | MsG0880047027.01.T01:CDS |
| GCATAATAAAGGAACAAGAT+AGG | 0.472463 | 8:-81163119 | MsG0880047027.01.T01:CDS |
| TTGCTACCAATAGCAAATGT+TGG | 0.481402 | 8:-81163096 | MsG0880047027.01.T01:CDS |
| TGCTACCAATAGCAAATGTT+GGG | 0.486912 | 8:-81163095 | MsG0880047027.01.T01:CDS |
| GAAAGGAGAAAGACAGTGAA+TGG | 0.488041 | 8:-81162946 | MsG0880047027.01.T01:CDS |
| ATGATATCTGTTGGGCTTTG+GGG | 0.489965 | 8:-81162921 | MsG0880047027.01.T01:CDS |
| GTGAGGCATCAGAGAAATGC+AGG | 0.490175 | 8:-81162972 | MsG0880047027.01.T01:CDS |
| TCCTTTAGTAATGATGAAAA+CGG | 0.512602 | 8:-81163144 | MsG0880047027.01.T01:CDS |
| CAGAGAAATGCAGGAAGGAA+AGG | 0.518431 | 8:-81162963 | MsG0880047027.01.T01:CDS |
| GAATGGTGATGATATCTGTT+GGG | 0.569420 | 8:-81162929 | MsG0880047027.01.T01:CDS |
| TATAAGCTTTGTGACAAGTG+AGG | 0.602260 | 8:-81162989 | MsG0880047027.01.T01:CDS |
| ATGATGGATAACAACGTTGG+AGG | 0.607529 | 8:-81163171 | MsG0880047027.01.T01:CDS |
| TGAATGGTGATGATATCTGT+TGG | 0.622578 | 8:-81162930 | MsG0880047027.01.T01:CDS |
| GAAATGATGGATAACAACGT+TGG | 0.629287 | 8:-81163174 | MsG0880047027.01.T01:CDS |
| TTTAGTAATGATGAAAACGG+AGG | 0.646824 | 8:-81163141 | MsG0880047027.01.T01:CDS |
| ATTACGCAGAACCAATGAGA+AGG | 0.655098 | 8:-81162882 | MsG0880047027.01.T01:CDS |
| TACCAATAGCAAATGTTGGG+AGG | 0.664077 | 8:-81163092 | MsG0880047027.01.T01:CDS |
| GGCATCAGAGAAATGCAGGA+AGG | 0.689891 | 8:-81162968 | MsG0880047027.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAAATCTCAAAGGAATCAA+AGG | - | Chr8:81162935-81162954 | MsG0880047027.01.T01:CDS | 25.0% |
| ! | TCCTTTAGTAATGATGAAAA+CGG | - | Chr8:81162831-81162850 | MsG0880047027.01.T01:CDS | 25.0% |
| ACAGAATGCAAAAATCTCAA+AGG | - | Chr8:81162926-81162945 | MsG0880047027.01.T01:CDS | 30.0% | |
| GCATAATAAAGGAACAAGAT+AGG | - | Chr8:81162856-81162875 | MsG0880047027.01.T01:CDS | 30.0% | |
| GCATAGATATAGAGAATTAG+AGG | - | Chr8:81163121-81163140 | MsG0880047027.01.T01:CDS | 30.0% | |
| GCTAATAATATTCCAGATAG+AGG | - | Chr8:81163164-81163183 | MsG0880047027.01.T01:CDS | 30.0% | |
| GGAATATTATTAGCAGTAGT+TGG | + | Chr8:81163158-81163177 | None:intergenic | 30.0% | |
| TTTAGTAATGATGAAAACGG+AGG | - | Chr8:81162834-81162853 | MsG0880047027.01.T01:CDS | 30.0% | |
| ! | TCCGTTTTCATCATTACTAA+AGG | + | Chr8:81162835-81162854 | None:intergenic | 30.0% |
| !! | TTTGAGATTTTTGCATTCTG+TGG | + | Chr8:81162927-81162946 | None:intergenic | 30.0% |
| CTATGCAAATACCTTCTCAT+TGG | + | Chr8:81163107-81163126 | None:intergenic | 35.0% | |
| TGCTACCAATAGCAAATGTT+GGG | - | Chr8:81162880-81162899 | MsG0880047027.01.T01:CDS | 35.0% | |
| TTGCTACCAATAGCAAATGT+TGG | - | Chr8:81162879-81162898 | MsG0880047027.01.T01:CDS | 35.0% | |
| ! | GAATGGTGATGATATCTGTT+GGG | - | Chr8:81163046-81163065 | MsG0880047027.01.T01:CDS | 35.0% |
| ! | TATAAGCTTTGTGACAAGTG+AGG | - | Chr8:81162986-81163005 | MsG0880047027.01.T01:CDS | 35.0% |
| ! | TGAAAACGGAGGCATAATAA+AGG | - | Chr8:81162845-81162864 | MsG0880047027.01.T01:CDS | 35.0% |
| ! | TGAATGGTGATGATATCTGT+TGG | - | Chr8:81163045-81163064 | MsG0880047027.01.T01:CDS | 35.0% |
| ATGATATCTGTTGGGCTTTG+GGG | - | Chr8:81163054-81163073 | MsG0880047027.01.T01:CDS | 40.0% | |
| ATTACGCAGAACCAATGAGA+AGG | - | Chr8:81163093-81163112 | MsG0880047027.01.T01:CDS | 40.0% | |
| GAAAGGAGAAAGACAGTGAA+TGG | - | Chr8:81163029-81163048 | MsG0880047027.01.T01:CDS | 40.0% | |
| GATGATATCTGTTGGGCTTT+GGG | - | Chr8:81163053-81163072 | MsG0880047027.01.T01:CDS | 40.0% | |
| TACCAATAGCAAATGTTGGG+AGG | - | Chr8:81162883-81162902 | MsG0880047027.01.T01:CDS | 40.0% | |
| TGATGATATCTGTTGGGCTT+TGG | - | Chr8:81163052-81163071 | MsG0880047027.01.T01:CDS | 40.0% | |
| !! | ATCCTCCCAACATTTGCTAT+TGG | + | Chr8:81162888-81162907 | None:intergenic | 40.0% |
| !! | ATGATGGATAACAACGTTGG+AGG | - | Chr8:81162804-81162823 | MsG0880047027.01.T01:CDS | 40.0% |
| CAGAGAAATGCAGGAAGGAA+AGG | - | Chr8:81163012-81163031 | MsG0880047027.01.T01:CDS | 45.0% | |
| GGCATCAGAGAAATGCAGGA+AGG | - | Chr8:81163007-81163026 | MsG0880047027.01.T01:CDS | 50.0% | |
| ! | GTGAGGCATCAGAGAAATGC+AGG | - | Chr8:81163003-81163022 | MsG0880047027.01.T01:CDS | 50.0% |
| !! | TGTTGGGCTTTGGGGACACT+TGG | - | Chr8:81163062-81163081 | MsG0880047027.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 81162804 | 81163193 | 81162804 | ID=MsG0880047027.01;Name=MsG0880047027.01 |
| Chr8 | mRNA | 81162804 | 81163193 | 81162804 | ID=MsG0880047027.01.T01;Parent=MsG0880047027.01;Name=MsG0880047027.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|129 |
| Chr8 | exon | 81162804 | 81163193 | 81162804 | ID=MsG0880047027.01.T01:exon:10590;Parent=MsG0880047027.01.T01 |
| Chr8 | CDS | 81162804 | 81163193 | 81162804 | ID=MsG0880047027.01.T01:cds;Parent=MsG0880047027.01.T01 |
| Gene Sequence |
| Protein sequence |
