Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047029.01.T01 | XP_024637179.1 | 88.55 | 393 | 41 | 2 | 1 | 390 | 1 | 392 | 0 | 672 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047029.01.T01 | G7JKC7 | 88.550 | 393 | 41 | 2 | 1 | 390 | 1 | 392 | 0.0 | 672 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0880047029.01.T01 | TR | mTERF |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047029.01.T01 | MTR_4g119550 | 88.550 | 393 | 41 | 2 | 1 | 390 | 1 | 392 | 0.0 | 672 |
| MsG0880047029.01.T01 | MTR_2g437020 | 52.809 | 356 | 162 | 4 | 37 | 388 | 27 | 380 | 1.45e-125 | 367 |
| MsG0880047029.01.T01 | MTR_2g436400 | 50.811 | 370 | 175 | 4 | 15 | 382 | 17 | 381 | 1.41e-124 | 365 |
| MsG0880047029.01.T01 | MTR_2g437060 | 50.562 | 356 | 173 | 2 | 35 | 388 | 41 | 395 | 1.75e-124 | 365 |
| MsG0880047029.01.T01 | MTR_2g437080 | 51.977 | 354 | 167 | 2 | 37 | 388 | 28 | 380 | 9.91e-124 | 362 |
| MsG0880047029.01.T01 | MTR_2g437040 | 49.153 | 354 | 177 | 2 | 37 | 388 | 28 | 380 | 1.36e-121 | 357 |
| MsG0880047029.01.T01 | MTR_2g437030 | 51.130 | 354 | 170 | 2 | 37 | 388 | 114 | 466 | 5.75e-121 | 358 |
| MsG0880047029.01.T01 | MTR_2g436440 | 46.825 | 378 | 194 | 3 | 16 | 387 | 9 | 385 | 1.13e-118 | 350 |
| MsG0880047029.01.T01 | MTR_2g437240 | 48.205 | 390 | 162 | 4 | 35 | 386 | 19 | 406 | 4.43e-118 | 349 |
| MsG0880047029.01.T01 | MTR_2g437200 | 47.520 | 383 | 160 | 4 | 36 | 378 | 17 | 398 | 2.59e-117 | 347 |
| MsG0880047029.01.T01 | MTR_2g437130 | 51.705 | 352 | 169 | 1 | 35 | 386 | 29 | 379 | 3.03e-115 | 341 |
| MsG0880047029.01.T01 | MTR_2g437260 | 45.682 | 359 | 190 | 3 | 29 | 383 | 14 | 371 | 8.26e-112 | 332 |
| MsG0880047029.01.T01 | MTR_2g436460 | 48.529 | 340 | 173 | 2 | 43 | 381 | 41 | 379 | 1.63e-110 | 329 |
| MsG0880047029.01.T01 | MTR_2g437990 | 48.267 | 375 | 180 | 6 | 25 | 386 | 17 | 390 | 8.69e-110 | 328 |
| MsG0880047029.01.T01 | MTR_2g437180 | 48.703 | 347 | 170 | 4 | 45 | 386 | 45 | 388 | 9.48e-110 | 327 |
| MsG0880047029.01.T01 | MTR_2g437960 | 48.116 | 345 | 173 | 4 | 49 | 388 | 53 | 396 | 1.00e-108 | 325 |
| MsG0880047029.01.T01 | MTR_2g076320 | 47.872 | 376 | 181 | 6 | 25 | 386 | 17 | 391 | 1.05e-108 | 325 |
| MsG0880047029.01.T01 | MTR_2g437960 | 48.116 | 345 | 173 | 4 | 49 | 388 | 53 | 396 | 2.62e-108 | 325 |
| MsG0880047029.01.T01 | MTR_2g438020 | 46.400 | 375 | 162 | 3 | 48 | 384 | 70 | 443 | 9.08e-108 | 326 |
| MsG0880047029.01.T01 | MTR_2g437120 | 48.148 | 351 | 175 | 5 | 41 | 386 | 43 | 391 | 1.38e-107 | 323 |
| MsG0880047029.01.T01 | MTR_2g438010 | 46.011 | 376 | 188 | 5 | 25 | 386 | 37 | 411 | 2.34e-107 | 322 |
| MsG0880047029.01.T01 | MTR_2g437150 | 46.234 | 385 | 191 | 8 | 17 | 386 | 15 | 398 | 2.19e-105 | 317 |
| MsG0880047029.01.T01 | MTR_2g437160 | 47.324 | 355 | 178 | 5 | 40 | 386 | 50 | 403 | 2.51e-105 | 317 |
| MsG0880047029.01.T01 | MTR_2g437170 | 46.335 | 382 | 189 | 8 | 20 | 386 | 18 | 398 | 1.10e-104 | 315 |
| MsG0880047029.01.T01 | MTR_2g019840 | 46.612 | 369 | 185 | 6 | 22 | 387 | 21 | 380 | 1.53e-104 | 314 |
| MsG0880047029.01.T01 | MTR_2g437100 | 46.175 | 366 | 178 | 6 | 31 | 378 | 31 | 395 | 2.41e-101 | 306 |
| MsG0880047029.01.T01 | MTR_4g119580 | 47.493 | 339 | 174 | 2 | 41 | 376 | 33 | 370 | 5.28e-100 | 302 |
| MsG0880047029.01.T01 | MTR_2g019810 | 47.590 | 332 | 169 | 4 | 49 | 378 | 31 | 359 | 5.43e-99 | 299 |
| MsG0880047029.01.T01 | MTR_4g120380 | 44.012 | 334 | 186 | 1 | 43 | 376 | 38 | 370 | 2.27e-95 | 290 |
| MsG0880047029.01.T01 | MTR_2g437940 | 42.965 | 398 | 179 | 9 | 1 | 386 | 1 | 362 | 6.56e-94 | 286 |
| MsG0880047029.01.T01 | MTR_4g120380 | 44.904 | 314 | 172 | 1 | 43 | 356 | 38 | 350 | 8.86e-91 | 280 |
| MsG0880047029.01.T01 | MTR_3g450500 | 34.731 | 334 | 209 | 4 | 50 | 376 | 39 | 370 | 1.54e-65 | 213 |
| MsG0880047029.01.T01 | MTR_2g437090 | 52.381 | 168 | 80 | 0 | 221 | 388 | 1 | 168 | 3.32e-61 | 195 |
| MsG0880047029.01.T01 | MTR_2g437160 | 44.248 | 226 | 110 | 1 | 177 | 386 | 87 | 312 | 2.36e-60 | 198 |
| MsG0880047029.01.T01 | MTR_4g007490 | 33.146 | 356 | 227 | 6 | 30 | 376 | 15 | 368 | 5.35e-57 | 191 |
| MsG0880047029.01.T01 | MTR_4g119570 | 39.759 | 249 | 117 | 1 | 128 | 376 | 14 | 229 | 8.87e-56 | 183 |
| MsG0880047029.01.T01 | MTR_2g436370 | 42.781 | 187 | 96 | 4 | 13 | 197 | 18 | 195 | 9.75e-38 | 135 |
| MsG0880047029.01.T01 | MTR_8g080990 | 25.526 | 333 | 243 | 4 | 44 | 372 | 37 | 368 | 2.93e-29 | 117 |
| MsG0880047029.01.T01 | MTR_8g081000 | 24.926 | 337 | 248 | 4 | 44 | 376 | 50 | 385 | 8.65e-28 | 113 |
| MsG0880047029.01.T01 | MTR_8g081000 | 24.926 | 337 | 248 | 4 | 44 | 376 | 50 | 385 | 1.20e-27 | 113 |
| MsG0880047029.01.T01 | MTR_8g081000 | 24.926 | 337 | 248 | 4 | 44 | 376 | 50 | 385 | 1.24e-27 | 113 |
| MsG0880047029.01.T01 | MTR_2g436380 | 37.931 | 116 | 63 | 4 | 221 | 335 | 1 | 108 | 7.38e-18 | 83.6 |
| MsG0880047029.01.T01 | MTR_4g049400 | 41.228 | 114 | 60 | 3 | 40 | 147 | 37 | 149 | 3.35e-17 | 80.5 |
| MsG0880047029.01.T01 | MTR_2g019830 | 51.852 | 81 | 38 | 1 | 303 | 383 | 125 | 204 | 2.64e-16 | 77.4 |
| MsG0880047029.01.T01 | MTR_3g085240 | 44.944 | 89 | 32 | 1 | 280 | 368 | 176 | 247 | 2.33e-14 | 72.8 |
| MsG0880047029.01.T01 | MTR_3g085240 | 37.903 | 124 | 47 | 2 | 33 | 156 | 42 | 135 | 2.98e-14 | 72.4 |
| MsG0880047029.01.T01 | MTR_4g119615 | 53.061 | 49 | 23 | 0 | 320 | 368 | 26 | 74 | 1.59e-11 | 60.8 |
| MsG0880047029.01.T01 | MTR_8g012210 | 22.414 | 290 | 215 | 7 | 93 | 376 | 63 | 348 | 7.25e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047029.01.T01 | AT5G07900 | 34.337 | 332 | 211 | 5 | 50 | 375 | 69 | 399 | 8.40e-67 | 217 |
| MsG0880047029.01.T01 | AT1G21150 | 33.535 | 331 | 213 | 4 | 49 | 373 | 55 | 384 | 2.30e-53 | 182 |
| MsG0880047029.01.T01 | AT1G21150 | 33.535 | 331 | 213 | 4 | 49 | 373 | 119 | 448 | 8.76e-53 | 182 |
| MsG0880047029.01.T01 | AT1G21150 | 32.831 | 332 | 216 | 4 | 48 | 373 | 18 | 348 | 5.05e-52 | 177 |
| MsG0880047029.01.T01 | AT1G61970 | 30.056 | 356 | 201 | 8 | 48 | 361 | 50 | 399 | 1.44e-39 | 146 |
| MsG0880047029.01.T01 | AT1G61970 | 30.056 | 356 | 201 | 8 | 48 | 361 | 50 | 399 | 1.44e-39 | 146 |
| MsG0880047029.01.T01 | AT1G61970 | 30.056 | 356 | 201 | 8 | 48 | 361 | 50 | 399 | 1.44e-39 | 146 |
| MsG0880047029.01.T01 | AT1G61970 | 30.056 | 356 | 201 | 8 | 48 | 361 | 50 | 399 | 1.44e-39 | 146 |
| MsG0880047029.01.T01 | AT1G61970 | 30.056 | 356 | 201 | 8 | 48 | 361 | 50 | 399 | 1.44e-39 | 146 |
| MsG0880047029.01.T01 | AT1G61970 | 30.056 | 356 | 201 | 8 | 48 | 361 | 50 | 399 | 1.44e-39 | 146 |
| MsG0880047029.01.T01 | AT1G61970 | 30.056 | 356 | 201 | 8 | 48 | 361 | 50 | 399 | 1.44e-39 | 146 |
| MsG0880047029.01.T01 | AT1G61980 | 29.545 | 352 | 206 | 8 | 50 | 362 | 52 | 400 | 4.84e-38 | 142 |
| MsG0880047029.01.T01 | AT1G61980 | 29.545 | 352 | 206 | 8 | 50 | 362 | 52 | 400 | 4.84e-38 | 142 |
| MsG0880047029.01.T01 | AT1G61980 | 29.545 | 352 | 206 | 8 | 50 | 362 | 52 | 400 | 4.84e-38 | 142 |
| MsG0880047029.01.T01 | AT1G61990 | 28.767 | 365 | 211 | 7 | 51 | 375 | 53 | 408 | 4.98e-36 | 136 |
| MsG0880047029.01.T01 | AT1G61990 | 28.767 | 365 | 211 | 7 | 51 | 375 | 53 | 408 | 4.98e-36 | 136 |
| MsG0880047029.01.T01 | AT1G62120 | 27.913 | 369 | 214 | 10 | 48 | 372 | 54 | 414 | 2.68e-34 | 132 |
| MsG0880047029.01.T01 | AT1G62110 | 29.452 | 292 | 173 | 5 | 51 | 316 | 53 | 337 | 4.07e-31 | 124 |
| MsG0880047029.01.T01 | AT3G46950 | 31.048 | 248 | 165 | 5 | 44 | 290 | 46 | 288 | 4.63e-30 | 120 |
| MsG0880047029.01.T01 | AT1G62085 | 27.577 | 388 | 204 | 10 | 50 | 362 | 55 | 440 | 9.37e-30 | 120 |
| MsG0880047029.01.T01 | AT1G62085 | 27.577 | 388 | 204 | 10 | 50 | 362 | 55 | 440 | 9.37e-30 | 120 |
| MsG0880047029.01.T01 | AT5G64950 | 26.879 | 346 | 240 | 5 | 43 | 376 | 32 | 376 | 1.46e-28 | 115 |
| MsG0880047029.01.T01 | AT1G61960 | 27.881 | 269 | 186 | 5 | 28 | 290 | 27 | 293 | 2.62e-27 | 113 |
| MsG0880047029.01.T01 | AT1G62150 | 24.819 | 415 | 227 | 12 | 45 | 383 | 53 | 458 | 3.33e-24 | 104 |
| MsG0880047029.01.T01 | AT5G23930 | 22.931 | 423 | 240 | 8 | 19 | 371 | 35 | 441 | 1.35e-22 | 99.4 |
| MsG0880047029.01.T01 | AT1G62010 | 25.444 | 338 | 202 | 10 | 76 | 372 | 64 | 392 | 1.55e-22 | 99.0 |
| MsG0880047029.01.T01 | AT1G56380 | 23.181 | 371 | 226 | 11 | 51 | 372 | 17 | 377 | 9.85e-18 | 84.7 |
| MsG0880047029.01.T01 | AT1G56380 | 23.181 | 371 | 226 | 11 | 51 | 372 | 28 | 388 | 1.22e-17 | 84.3 |
| MsG0880047029.01.T01 | AT1G79220 | 23.737 | 396 | 265 | 14 | 6 | 388 | 18 | 389 | 1.57e-12 | 68.9 |
Find 78 sgRNAs with CRISPR-Local
Find 96 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATAACGATTAATACATTTAA+TGG | 0.168353 | 8:-81184320 | None:intergenic |
| GCGGAAATTGAAATTGAAAT+TGG | 0.211958 | 8:-81183903 | None:intergenic |
| TCAGTTAGGTTTGAATTCTT+TGG | 0.234752 | 8:+81184702 | MsG0880047029.01.T01:CDS |
| TATGACAGAGAAGTTGTTTC+TGG | 0.244337 | 8:+81184855 | MsG0880047029.01.T01:CDS |
| GTCAAATTATTGCTTGATAA+TGG | 0.274159 | 8:+81184379 | MsG0880047029.01.T01:CDS |
| TCATTTGTTGAAGCTGTATC+AGG | 0.289926 | 8:+81184912 | MsG0880047029.01.T01:CDS |
| AACACATTCTTCAAGCATTT+AGG | 0.305458 | 8:+81184614 | MsG0880047029.01.T01:CDS |
| AAACTTCTTAAGAGGAATCT+AGG | 0.329404 | 8:-81184235 | None:intergenic |
| TTTGGTAAAAGTACCTTGTT+GGG | 0.357862 | 8:-81184148 | None:intergenic |
| GATTGGGCAAACTTCTTAAG+AGG | 0.387256 | 8:-81184243 | None:intergenic |
| CAACCTGTTGGAGACTGTTC+AGG | 0.388932 | 8:+81184462 | MsG0880047029.01.T01:CDS |
| ATCAGAAGTAGAAGCACCTT+TGG | 0.391353 | 8:-81184188 | None:intergenic |
| CTTTGGTAAAAGTACCTTGT+TGG | 0.403823 | 8:-81184149 | None:intergenic |
| ACCTTACTGTATGTTGTCTT+CGG | 0.415933 | 8:+81184642 | MsG0880047029.01.T01:CDS |
| CCACAGTTCCCTGGTTCGTA+AGG | 0.423694 | 8:+81183823 | MsG0880047029.01.T01:CDS |
| TTGCAGTTCCTTGTATCTAA+AGG | 0.428240 | 8:+81184793 | MsG0880047029.01.T01:CDS |
| ATGTTGCTGTTGTGGTGAAT+AGG | 0.436111 | 8:-81183856 | None:intergenic |
| AAATTCGTAATATCATCAAA+AGG | 0.437298 | 8:+81184098 | MsG0880047029.01.T01:CDS |
| AGCGCTTGGGTTGATCAGTT+AGG | 0.440546 | 8:+81184688 | MsG0880047029.01.T01:CDS |
| ATAAGTAGGGATGATATGAT+TGG | 0.445105 | 8:-81184260 | None:intergenic |
| AACTGCAAAACTGCAGCCCT+TGG | 0.446416 | 8:-81184778 | None:intergenic |
| TTTGGATCTTGTTAATGCTC+CGG | 0.453825 | 8:+81184720 | MsG0880047029.01.T01:CDS |
| TTTATTGAGACTCGAGAAAC+GGG | 0.454508 | 8:+81184750 | MsG0880047029.01.T01:CDS |
| GAAGAGGGCGATAACTGAAT+CGG | 0.468774 | 8:-81184050 | None:intergenic |
| AAACATTGAGTGCGGTTTGA+GGG | 0.473082 | 8:-81183994 | None:intergenic |
| ATTCCTGAACAGTCTCCAAC+AGG | 0.475079 | 8:-81184465 | None:intergenic |
| TTGGATCTTGTTAATGCTCC+GGG | 0.475407 | 8:+81184721 | MsG0880047029.01.T01:CDS |
| TTCTCAAACCTTTAGATACA+AGG | 0.476085 | 8:-81184801 | None:intergenic |
| CCTCTCATAAAATGATAAGT+AGG | 0.495753 | 8:-81184274 | None:intergenic |
| AACTTCTCTGTCATAGCAAA+CGG | 0.496374 | 8:-81184847 | None:intergenic |
| AAAGTACCTTGTTGGGGTCG+AGG | 0.506736 | 8:-81184141 | None:intergenic |
| AAGAGGGCGATAACTGAATC+GGG | 0.512699 | 8:-81184049 | None:intergenic |
| AATTCGTAATATCATCAAAA+GGG | 0.515448 | 8:+81184099 | MsG0880047029.01.T01:CDS |
| CACAGTTCCCTGGTTCGTAA+GGG | 0.515923 | 8:+81183824 | MsG0880047029.01.T01:CDS |
| GAAACATTGAGTGCGGTTTG+AGG | 0.516511 | 8:-81183995 | None:intergenic |
| TTGGATGAACAATATATCCT+AGG | 0.518394 | 8:-81184442 | None:intergenic |
| ATTCGTCTCCCACAGTTCCC+TGG | 0.521388 | 8:+81183814 | MsG0880047029.01.T01:CDS |
| AACTTCTTAAGAGGAATCTA+GGG | 0.525099 | 8:-81184234 | None:intergenic |
| TAAGTAGGGATGATATGATT+GGG | 0.531463 | 8:-81184259 | None:intergenic |
| ATATTGTTCATCCAACCTGT+TGG | 0.532063 | 8:+81184450 | MsG0880047029.01.T01:CDS |
| GATATTACGAATTTGAGTGT+TGG | 0.541490 | 8:-81184089 | None:intergenic |
| ACATTCTTCAAGCATTTAGG+AGG | 0.543592 | 8:+81184617 | MsG0880047029.01.T01:CDS |
| GAAGAAAACCCTTACGAACC+AGG | 0.548906 | 8:-81183832 | None:intergenic |
| TCGAGAAACGGGTTATTCCA+AGG | 0.551680 | 8:+81184761 | MsG0880047029.01.T01:CDS |
| AATGAATCTTGCAAATAGCA+TGG | 0.553137 | 8:+81184939 | MsG0880047029.01.T01:CDS |
| CTCTCATAAAATGATAAGTA+GGG | 0.556083 | 8:-81184273 | None:intergenic |
| AGAAGTCGTAATTGTTGATG+AGG | 0.557806 | 8:-81183970 | None:intergenic |
| GTTCAGGAATTGAAGCAACT+AGG | 0.561440 | 8:+81184478 | MsG0880047029.01.T01:CDS |
| AGAAGTTACACACTTCATGA+AGG | 0.561506 | 8:-81184970 | None:intergenic |
| AACATTGAGTGCGGTTTGAG+GGG | 0.563771 | 8:-81183993 | None:intergenic |
| TGATACAGCTTCAACAAATG+AGG | 0.568471 | 8:-81184910 | None:intergenic |
| GACGGTGAAAGAATGAGAGG+TGG | 0.570009 | 8:-81183942 | None:intergenic |
| ATCGCCCTCTTCAACACCCA+TGG | 0.572102 | 8:+81184061 | MsG0880047029.01.T01:CDS |
| AGAAGCCATGGGTGTTGAAG+AGG | 0.578650 | 8:-81184066 | None:intergenic |
| CATTGTTGCGTAATTCGCCT+AGG | 0.579851 | 8:+81184425 | MsG0880047029.01.T01:CDS |
| AGAAAACTGTGAATGAAACT+AGG | 0.580330 | 8:+81184545 | MsG0880047029.01.T01:CDS |
| CTGAACAGTCTCCAACAGGT+TGG | 0.584404 | 8:-81184461 | None:intergenic |
| AGAGACGGTGAAAGAATGAG+AGG | 0.585401 | 8:-81183945 | None:intergenic |
| GCCGAAGACAACATACAGTA+AGG | 0.592002 | 8:-81184643 | None:intergenic |
| TTTGAGTGTTGGAGAAGCCA+TGG | 0.596210 | 8:-81184078 | None:intergenic |
| CCTTACGAACCAGGGAACTG+TGG | 0.599382 | 8:-81183823 | None:intergenic |
| GAAGCCATGGGTGTTGAAGA+GGG | 0.600289 | 8:-81184065 | None:intergenic |
| AGCAAACGGTGCACTTAGTG+AGG | 0.600976 | 8:-81184833 | None:intergenic |
| ACGTGTAGATGTTGCTGTTG+TGG | 0.602104 | 8:-81183864 | None:intergenic |
| CTTGCGAGAAACATTGAGTG+CGG | 0.609249 | 8:-81184002 | None:intergenic |
| AACTGTGAATGAAACTAGGT+GGG | 0.613433 | 8:+81184549 | MsG0880047029.01.T01:CDS |
| CGAGAAACGGGTTATTCCAA+GGG | 0.615093 | 8:+81184762 | MsG0880047029.01.T01:CDS |
| TGGTTGAAGTTGAAATGAAG+CGG | 0.625865 | 8:-81183922 | None:intergenic |
| AAGAAAACCCTTACGAACCA+GGG | 0.629010 | 8:-81183831 | None:intergenic |
| TTGAGTGTTGGAGAAGCCAT+GGG | 0.633074 | 8:-81184077 | None:intergenic |
| CTTATGCCTCGACCCCAACA+AGG | 0.635453 | 8:+81184135 | MsG0880047029.01.T01:CDS |
| TGTAGCAATATTTGAGTGTG+TGG | 0.635496 | 8:-81184404 | None:intergenic |
| AAACTGTGAATGAAACTAGG+TGG | 0.639268 | 8:+81184548 | MsG0880047029.01.T01:CDS |
| ATATCATCAAAAGGGAACCT+CGG | 0.640196 | 8:+81184107 | MsG0880047029.01.T01:CDS |
| CTTACGAACCAGGGAACTGT+GGG | 0.643291 | 8:-81183822 | None:intergenic |
| TTGGTAAAAGTACCTTGTTG+GGG | 0.646951 | 8:-81184147 | None:intergenic |
| ATTGTTGATGAGGTAAGAGA+CGG | 0.666398 | 8:-81183960 | None:intergenic |
| TCGAGGCATAAGAGTAACCG+AGG | 0.694428 | 8:-81184124 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAACGATTAATACATTTAA+TGG | - | Chr8:81184323-81184342 | None:intergenic | 15.0% |
| !!! | AAGTTGAAATTTTTAAGAAA+TGG | + | Chr8:81184578-81184597 | MsG0880047029.01.T01:CDS | 15.0% |
| !!! | AGTTGAAATTTTTAAGAAAT+GGG | + | Chr8:81184579-81184598 | MsG0880047029.01.T01:CDS | 15.0% |
| !! | AAATTCGTAATATCATCAAA+AGG | + | Chr8:81184098-81184117 | MsG0880047029.01.T01:CDS | 20.0% |
| !! | AATTCGTAATATCATCAAAA+GGG | + | Chr8:81184099-81184118 | MsG0880047029.01.T01:CDS | 20.0% |
| !! | GAAAGTAAAAATTGAAACTT+TGG | - | Chr8:81184169-81184188 | None:intergenic | 20.0% |
| !!! | CAAATTTGTGAAATGTTTTA+AGG | + | Chr8:81184879-81184898 | MsG0880047029.01.T01:CDS | 20.0% |
| !!! | GTTGAAATTTTTAAGAAATG+GGG | + | Chr8:81184580-81184599 | MsG0880047029.01.T01:CDS | 20.0% |
| !!! | TTTCAATTTTTACTTTCCAA+AGG | + | Chr8:81184172-81184191 | MsG0880047029.01.T01:CDS | 20.0% |
| ! | CTCTCATAAAATGATAAGTA+GGG | - | Chr8:81184276-81184295 | None:intergenic | 25.0% |
| ! | GTCAAATTATTGCTTGATAA+TGG | + | Chr8:81184379-81184398 | MsG0880047029.01.T01:CDS | 25.0% |
| !! | CTACTTATCATTTTATGAGA+GGG | + | Chr8:81184275-81184294 | MsG0880047029.01.T01:CDS | 25.0% |
| !!! | AAATTTTTAAGAAATGGGGT+TGG | + | Chr8:81184584-81184603 | MsG0880047029.01.T01:CDS | 25.0% |
| !!! | ATTTGTGAAATGTTTTAAGG+AGG | + | Chr8:81184882-81184901 | MsG0880047029.01.T01:CDS | 25.0% |
| AAACTTCTTAAGAGGAATCT+AGG | - | Chr8:81184238-81184257 | None:intergenic | 30.0% | |
| AACACATTCTTCAAGCATTT+AGG | + | Chr8:81184614-81184633 | MsG0880047029.01.T01:CDS | 30.0% | |
| AACTTCTTAAGAGGAATCTA+GGG | - | Chr8:81184237-81184256 | None:intergenic | 30.0% | |
| AATGAATCTTGCAAATAGCA+TGG | + | Chr8:81184939-81184958 | MsG0880047029.01.T01:CDS | 30.0% | |
| AGAAAACTGTGAATGAAACT+AGG | + | Chr8:81184545-81184564 | MsG0880047029.01.T01:CDS | 30.0% | |
| AGTCTCAATAAAAAGATTCC+CGG | - | Chr8:81184742-81184761 | None:intergenic | 30.0% | |
| ATAAGTAGGGATGATATGAT+TGG | - | Chr8:81184263-81184282 | None:intergenic | 30.0% | |
| CCTCTCATAAAATGATAAGT+AGG | - | Chr8:81184277-81184296 | None:intergenic | 30.0% | |
| GATATTACGAATTTGAGTGT+TGG | - | Chr8:81184092-81184111 | None:intergenic | 30.0% | |
| GCGGAAATTGAAATTGAAAT+TGG | - | Chr8:81183906-81183925 | None:intergenic | 30.0% | |
| TAAGTAGGGATGATATGATT+GGG | - | Chr8:81184262-81184281 | None:intergenic | 30.0% | |
| TCAGTTAGGTTTGAATTCTT+TGG | + | Chr8:81184702-81184721 | MsG0880047029.01.T01:CDS | 30.0% | |
| TTCTCAAACCTTTAGATACA+AGG | - | Chr8:81184804-81184823 | None:intergenic | 30.0% | |
| TTGGATGAACAATATATCCT+AGG | - | Chr8:81184445-81184464 | None:intergenic | 30.0% | |
| ! | CCTACTTATCATTTTATGAG+AGG | + | Chr8:81184274-81184293 | MsG0880047029.01.T01:CDS | 30.0% |
| ! | TTTTATTGAGACTCGAGAAA+CGG | + | Chr8:81184749-81184768 | MsG0880047029.01.T01:CDS | 30.0% |
| !! | TTTGGTAAAAGTACCTTGTT+GGG | - | Chr8:81184151-81184170 | None:intergenic | 30.0% |
| AAACTGTGAATGAAACTAGG+TGG | + | Chr8:81184548-81184567 | MsG0880047029.01.T01:CDS | 35.0% | |
| AACTGTGAATGAAACTAGGT+GGG | + | Chr8:81184549-81184568 | MsG0880047029.01.T01:CDS | 35.0% | |
| AACTTCTCTGTCATAGCAAA+CGG | - | Chr8:81184850-81184869 | None:intergenic | 35.0% | |
| ACATTCTTCAAGCATTTAGG+AGG | + | Chr8:81184617-81184636 | MsG0880047029.01.T01:CDS | 35.0% | |
| ACCTTACTGTATGTTGTCTT+CGG | + | Chr8:81184642-81184661 | MsG0880047029.01.T01:CDS | 35.0% | |
| AGAAGTCGTAATTGTTGATG+AGG | - | Chr8:81183973-81183992 | None:intergenic | 35.0% | |
| ATATCATCAAAAGGGAACCT+CGG | + | Chr8:81184107-81184126 | MsG0880047029.01.T01:CDS | 35.0% | |
| ATATTGTTCATCCAACCTGT+TGG | + | Chr8:81184450-81184469 | MsG0880047029.01.T01:CDS | 35.0% | |
| TGATACAGCTTCAACAAATG+AGG | - | Chr8:81184913-81184932 | None:intergenic | 35.0% | |
| TGGTTGAAGTTGAAATGAAG+CGG | - | Chr8:81183925-81183944 | None:intergenic | 35.0% | |
| TGTAGCAATATTTGAGTGTG+TGG | - | Chr8:81184407-81184426 | None:intergenic | 35.0% | |
| TTGCAGTTCCTTGTATCTAA+AGG | + | Chr8:81184793-81184812 | MsG0880047029.01.T01:CDS | 35.0% | |
| TTTATTGAGACTCGAGAAAC+GGG | + | Chr8:81184750-81184769 | MsG0880047029.01.T01:CDS | 35.0% | |
| TTTGGATCTTGTTAATGCTC+CGG | + | Chr8:81184720-81184739 | MsG0880047029.01.T01:CDS | 35.0% | |
| ! | ATTGTTGATGAGGTAAGAGA+CGG | - | Chr8:81183963-81183982 | None:intergenic | 35.0% |
| ! | GATAACTGAATCGGGTTTTT+GGG | - | Chr8:81184044-81184063 | None:intergenic | 35.0% |
| ! | TATGACAGAGAAGTTGTTTC+TGG | + | Chr8:81184855-81184874 | MsG0880047029.01.T01:CDS | 35.0% |
| ! | TCATTTGTTGAAGCTGTATC+AGG | + | Chr8:81184912-81184931 | MsG0880047029.01.T01:CDS | 35.0% |
| !! | CTTTGGTAAAAGTACCTTGT+TGG | - | Chr8:81184152-81184171 | None:intergenic | 35.0% |
| !! | TTGGTAAAAGTACCTTGTTG+GGG | - | Chr8:81184150-81184169 | None:intergenic | 35.0% |
| !! | TTTTAAGAAATGGGGTTGGT+CGG | + | Chr8:81184588-81184607 | MsG0880047029.01.T01:CDS | 35.0% |
| !!! | TTCAACTTTTGCTGTTGCAT+TGG | + | Chr8:81184516-81184535 | MsG0880047029.01.T01:CDS | 35.0% |
| AAACATTGAGTGCGGTTTGA+GGG | - | Chr8:81183997-81184016 | None:intergenic | 40.0% | |
| AAGAAAACCCTTACGAACCA+GGG | - | Chr8:81183834-81183853 | None:intergenic | 40.0% | |
| GATTGGGCAAACTTCTTAAG+AGG | - | Chr8:81184246-81184265 | None:intergenic | 40.0% | |
| GTTCAGGAATTGAAGCAACT+AGG | + | Chr8:81184478-81184497 | MsG0880047029.01.T01:CDS | 40.0% | |
| TTGGATCTTGTTAATGCTCC+GGG | + | Chr8:81184721-81184740 | MsG0880047029.01.T01:CDS | 40.0% | |
| ! | ATGTTGCTGTTGTGGTGAAT+AGG | - | Chr8:81183859-81183878 | None:intergenic | 40.0% |
| ! | CGATAACTGAATCGGGTTTT+TGG | - | Chr8:81184045-81184064 | None:intergenic | 40.0% |
| !! | ATCAGAAGTAGAAGCACCTT+TGG | - | Chr8:81184191-81184210 | None:intergenic | 40.0% |
| !! | TGAATCGGGTTTTTGGGAAT+CGG | - | Chr8:81184038-81184057 | None:intergenic | 40.0% |
| AACATTGAGTGCGGTTTGAG+GGG | - | Chr8:81183996-81184015 | None:intergenic | 45.0% | |
| AAGAGGGCGATAACTGAATC+GGG | - | Chr8:81184052-81184071 | None:intergenic | 45.0% | |
| AGAGACGGTGAAAGAATGAG+AGG | - | Chr8:81183948-81183967 | None:intergenic | 45.0% | |
| ATTCCTGAACAGTCTCCAAC+AGG | - | Chr8:81184468-81184487 | None:intergenic | 45.0% | |
| CATTGTTGCGTAATTCGCCT+AGG | + | Chr8:81184425-81184444 | MsG0880047029.01.T01:CDS | 45.0% | |
| CTTGCGAGAAACATTGAGTG+CGG | - | Chr8:81184005-81184024 | None:intergenic | 45.0% | |
| GAAACATTGAGTGCGGTTTG+AGG | - | Chr8:81183998-81184017 | None:intergenic | 45.0% | |
| GAAGAAAACCCTTACGAACC+AGG | - | Chr8:81183835-81183854 | None:intergenic | 45.0% | |
| GAAGAGGGCGATAACTGAAT+CGG | - | Chr8:81184053-81184072 | None:intergenic | 45.0% | |
| GCCGAAGACAACATACAGTA+AGG | - | Chr8:81184646-81184665 | None:intergenic | 45.0% | |
| ! | ACGTGTAGATGTTGCTGTTG+TGG | - | Chr8:81183867-81183886 | None:intergenic | 45.0% |
| !! | CGAGAAACGGGTTATTCCAA+GGG | + | Chr8:81184762-81184781 | MsG0880047029.01.T01:CDS | 45.0% |
| !! | TCGAGAAACGGGTTATTCCA+AGG | + | Chr8:81184761-81184780 | MsG0880047029.01.T01:CDS | 45.0% |
| !! | TTGAGTGTTGGAGAAGCCAT+GGG | - | Chr8:81184080-81184099 | None:intergenic | 45.0% |
| !! | TTTGAGTGTTGGAGAAGCCA+TGG | - | Chr8:81184081-81184100 | None:intergenic | 45.0% |
| AACTGCAAAACTGCAGCCCT+TGG | - | Chr8:81184781-81184800 | None:intergenic | 50.0% | |
| AGCAAACGGTGCACTTAGTG+AGG | - | Chr8:81184836-81184855 | None:intergenic | 50.0% | |
| CAACCTGTTGGAGACTGTTC+AGG | + | Chr8:81184462-81184481 | MsG0880047029.01.T01:CDS | 50.0% | |
| CTGAACAGTCTCCAACAGGT+TGG | - | Chr8:81184464-81184483 | None:intergenic | 50.0% | |
| CTTACGAACCAGGGAACTGT+GGG | - | Chr8:81183825-81183844 | None:intergenic | 50.0% | |
| GACGGTGAAAGAATGAGAGG+TGG | - | Chr8:81183945-81183964 | None:intergenic | 50.0% | |
| TCGAGGCATAAGAGTAACCG+AGG | - | Chr8:81184127-81184146 | None:intergenic | 50.0% | |
| ! | AAAGTACCTTGTTGGGGTCG+AGG | - | Chr8:81184144-81184163 | None:intergenic | 50.0% |
| ! | CACAGTTCCCTGGTTCGTAA+GGG | + | Chr8:81183824-81183843 | MsG0880047029.01.T01:CDS | 50.0% |
| !! | AGAAGCCATGGGTGTTGAAG+AGG | - | Chr8:81184069-81184088 | None:intergenic | 50.0% |
| !! | AGCGCTTGGGTTGATCAGTT+AGG | + | Chr8:81184688-81184707 | MsG0880047029.01.T01:CDS | 50.0% |
| !! | GAAGCCATGGGTGTTGAAGA+GGG | - | Chr8:81184068-81184087 | None:intergenic | 50.0% |
| !!! | CGATGCAGTTTTGAGCGCTT+GGG | + | Chr8:81184675-81184694 | MsG0880047029.01.T01:CDS | 50.0% |
| !!! | TCGATGCAGTTTTGAGCGCT+TGG | + | Chr8:81184674-81184693 | MsG0880047029.01.T01:CDS | 50.0% |
| ATCGCCCTCTTCAACACCCA+TGG | + | Chr8:81184061-81184080 | MsG0880047029.01.T01:CDS | 55.0% | |
| ATTCGTCTCCCACAGTTCCC+TGG | + | Chr8:81183814-81183833 | MsG0880047029.01.T01:CDS | 55.0% | |
| CCTTACGAACCAGGGAACTG+TGG | - | Chr8:81183826-81183845 | None:intergenic | 55.0% | |
| CTTATGCCTCGACCCCAACA+AGG | + | Chr8:81184135-81184154 | MsG0880047029.01.T01:CDS | 55.0% | |
| ! | CCACAGTTCCCTGGTTCGTA+AGG | + | Chr8:81183823-81183842 | MsG0880047029.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 81183806 | 81184978 | 81183806 | ID=MsG0880047029.01;Name=MsG0880047029.01 |
| Chr8 | mRNA | 81183806 | 81184978 | 81183806 | ID=MsG0880047029.01.T01;Parent=MsG0880047029.01;Name=MsG0880047029.01.T01;_AED=0.41;_eAED=0.41;_QI=0|-1|0|1|-1|1|1|0|390 |
| Chr8 | exon | 81183806 | 81184978 | 81183806 | ID=MsG0880047029.01.T01:exon:10560;Parent=MsG0880047029.01.T01 |
| Chr8 | CDS | 81183806 | 81184978 | 81183806 | ID=MsG0880047029.01.T01:cds;Parent=MsG0880047029.01.T01 |
| Gene Sequence |
| Protein sequence |
