Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047224.01.T01 | XP_039683052.1 | 96.386 | 83 | 3 | 0 | 1 | 83 | 1 | 83 | 8.95E-49 | 174 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047224.01.T01 | Q9M8J3 | 46.753 | 77 | 27 | 2 | 8 | 83 | 27 | 90 | 1.15E-12 | 64.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047224.01.T01 | A0A2K3PRH2 | 72.289 | 83 | 23 | 0 | 1 | 83 | 34 | 116 | 8.40e-35 | 135 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0880047224.01.T01 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047224.01.T01 | MTR_4g102720 | 53.165 | 79 | 37 | 0 | 4 | 82 | 2 | 80 | 5.13e-24 | 90.5 |
| MsG0880047224.01.T01 | MTR_1g057920 | 50.667 | 75 | 36 | 1 | 9 | 82 | 34 | 108 | 1.26e-17 | 71.6 |
| MsG0880047224.01.T01 | MTR_5g015480 | 50.667 | 75 | 36 | 1 | 9 | 82 | 56 | 130 | 5.95e-17 | 72.8 |
| MsG0880047224.01.T01 | MTR_7g061980 | 39.024 | 82 | 49 | 1 | 3 | 83 | 4 | 85 | 1.51e-16 | 68.9 |
| MsG0880047224.01.T01 | MTR_7g074790 | 56.897 | 58 | 25 | 0 | 6 | 63 | 7 | 64 | 2.58e-16 | 70.5 |
| MsG0880047224.01.T01 | MTR_6g017200 | 49.333 | 75 | 37 | 1 | 9 | 82 | 58 | 132 | 2.89e-16 | 68.9 |
| MsG0880047224.01.T01 | MTR_2g098920 | 42.105 | 76 | 42 | 2 | 8 | 82 | 79 | 153 | 4.11e-15 | 67.8 |
| MsG0880047224.01.T01 | MTR_4g067290 | 39.474 | 76 | 44 | 2 | 8 | 82 | 55 | 129 | 1.01e-14 | 66.6 |
| MsG0880047224.01.T01 | MTR_4g067290 | 39.474 | 76 | 44 | 2 | 8 | 82 | 55 | 129 | 1.15e-14 | 66.2 |
| MsG0880047224.01.T01 | MTR_2g070590 | 45.333 | 75 | 40 | 1 | 9 | 82 | 17 | 91 | 7.76e-14 | 62.0 |
| MsG0880047224.01.T01 | MTR_4g067290 | 39.474 | 76 | 44 | 2 | 8 | 82 | 73 | 147 | 1.07e-13 | 63.9 |
| MsG0880047224.01.T01 | MTR_4g067290 | 39.474 | 76 | 44 | 2 | 8 | 82 | 75 | 149 | 1.09e-13 | 63.9 |
| MsG0880047224.01.T01 | MTR_4g067290 | 39.474 | 76 | 44 | 2 | 8 | 82 | 75 | 149 | 1.09e-13 | 63.9 |
| MsG0880047224.01.T01 | MTR_4g067290 | 39.474 | 76 | 44 | 2 | 8 | 82 | 115 | 189 | 1.10e-13 | 64.3 |
| MsG0880047224.01.T01 | MTR_0402s0040 | 48.000 | 75 | 38 | 1 | 9 | 82 | 26 | 100 | 1.68e-13 | 64.3 |
| MsG0880047224.01.T01 | MTR_5g039760 | 38.158 | 76 | 46 | 1 | 6 | 80 | 7 | 82 | 4.80e-12 | 58.5 |
| MsG0880047224.01.T01 | MTR_2g098880 | 50.877 | 57 | 27 | 1 | 7 | 62 | 59 | 115 | 2.32e-11 | 57.8 |
| MsG0880047224.01.T01 | MTR_2g098880 | 51.786 | 56 | 26 | 1 | 8 | 62 | 72 | 127 | 3.63e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047224.01.T01 | AT3G06250 | 46.753 | 77 | 27 | 2 | 8 | 83 | 27 | 90 | 1.17e-13 | 64.7 |
| MsG0880047224.01.T01 | AT3G06250 | 46.032 | 63 | 33 | 1 | 8 | 69 | 189 | 251 | 4.97e-11 | 57.4 |
| MsG0880047224.01.T01 | AT3G06250 | 46.753 | 77 | 27 | 2 | 8 | 83 | 27 | 90 | 1.17e-13 | 64.7 |
| MsG0880047224.01.T01 | AT3G06250 | 46.032 | 63 | 33 | 1 | 8 | 69 | 189 | 251 | 4.97e-11 | 57.4 |
| MsG0880047224.01.T01 | AT3G06250 | 46.753 | 77 | 27 | 2 | 8 | 83 | 27 | 90 | 1.17e-13 | 64.7 |
| MsG0880047224.01.T01 | AT3G06250 | 46.032 | 63 | 33 | 1 | 8 | 69 | 189 | 251 | 4.97e-11 | 57.4 |
| MsG0880047224.01.T01 | AT3G59470 | 39.474 | 76 | 44 | 2 | 8 | 82 | 69 | 143 | 1.91e-13 | 63.2 |
| MsG0880047224.01.T01 | AT3G59470 | 39.474 | 76 | 44 | 2 | 8 | 82 | 69 | 143 | 1.91e-13 | 63.2 |
| MsG0880047224.01.T01 | AT5G18960 | 50.794 | 63 | 30 | 1 | 8 | 69 | 42 | 104 | 3.57e-13 | 63.2 |
| MsG0880047224.01.T01 | AT5G18960 | 50.794 | 63 | 30 | 1 | 8 | 69 | 42 | 104 | 3.57e-13 | 63.2 |
| MsG0880047224.01.T01 | AT3G59470 | 39.474 | 76 | 44 | 2 | 8 | 82 | 81 | 155 | 9.49e-13 | 61.6 |
Find 12 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGGAAAACAATTTAATACT+TGG | 0.412515 | 8:+83556106 | MsG0880047224.01.T01:CDS |
| CATCGGCTTGAAATAAGAAC+TGG | 0.429549 | 8:+83556294 | MsG0880047224.01.T01:CDS |
| AGATTTGTGTGTAGTAAAGA+AGG | 0.465396 | 8:+83556231 | MsG0880047224.01.T01:CDS |
| CCACTCAAATTCGTTATCCA+TGG | 0.470045 | 8:-83556073 | None:intergenic |
| ACAATTTAATACTTGGGAAG+AGG | 0.492665 | 8:+83556113 | MsG0880047224.01.T01:CDS |
| TACTTGAACAAAAGCAAAGA+TGG | 0.497887 | 8:+83556192 | MsG0880047224.01.T01:CDS |
| GAACAAAAGCAAAGATGGAA+TGG | 0.523067 | 8:+83556197 | MsG0880047224.01.T01:CDS |
| AGGAAAACAATTTAATACTT+GGG | 0.524399 | 8:+83556107 | MsG0880047224.01.T01:CDS |
| TTAATACTTGGGAAGAGGCA+AGG | 0.554115 | 8:+83556118 | MsG0880047224.01.T01:CDS |
| ATTGCTTAACAACTAATCAT+CGG | 0.556534 | 8:+83556277 | MsG0880047224.01.T01:CDS |
| CCATGGATAACGAATTTGAG+TGG | 0.608327 | 8:+83556073 | None:intergenic |
| TTTGAGTGGAAACCTCGACT+AGG | 0.658485 | 8:+83556087 | MsG0880047224.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGGAAAACAATTTAATACTT+GGG | + | Chr8:83556107-83556126 | MsG0880047224.01.T01:CDS | 20.0% |
| !! | TAGGAAAACAATTTAATACT+TGG | + | Chr8:83556106-83556125 | MsG0880047224.01.T01:CDS | 20.0% |
| ! | ATTGCTTAACAACTAATCAT+CGG | + | Chr8:83556277-83556296 | MsG0880047224.01.T01:CDS | 25.0% |
| !! | AATGATTATGGTGGAAAAAT+TGG | + | Chr8:83556150-83556169 | MsG0880047224.01.T01:CDS | 25.0% |
| !!! | AATTTTTGGAATGATTATGG+TGG | + | Chr8:83556141-83556160 | MsG0880047224.01.T01:CDS | 25.0% |
| !!! | AGGAATTTTTGGAATGATTA+TGG | + | Chr8:83556138-83556157 | MsG0880047224.01.T01:CDS | 25.0% |
| ACAATTTAATACTTGGGAAG+AGG | + | Chr8:83556113-83556132 | MsG0880047224.01.T01:CDS | 30.0% | |
| AGATTTGTGTGTAGTAAAGA+AGG | + | Chr8:83556231-83556250 | MsG0880047224.01.T01:CDS | 30.0% | |
| TACTTGAACAAAAGCAAAGA+TGG | + | Chr8:83556192-83556211 | MsG0880047224.01.T01:CDS | 30.0% | |
| ! | GTTAAGCAATCTCTTTTATC+TGG | - | Chr8:83556267-83556286 | None:intergenic | 30.0% |
| ! | TTAAATTGTTTTCCTAGTCG+AGG | - | Chr8:83556102-83556121 | None:intergenic | 30.0% |
| GAACAAAAGCAAAGATGGAA+TGG | + | Chr8:83556197-83556216 | MsG0880047224.01.T01:CDS | 35.0% | |
| !!! | AAAGATGGAATGGTTTTGTC+TGG | + | Chr8:83556207-83556226 | MsG0880047224.01.T01:CDS | 35.0% |
| TTAATACTTGGGAAGAGGCA+AGG | + | Chr8:83556118-83556137 | MsG0880047224.01.T01:CDS | 40.0% | |
| !! | CATCGGCTTGAAATAAGAAC+TGG | + | Chr8:83556294-83556313 | MsG0880047224.01.T01:CDS | 40.0% |
| TTTGAGTGGAAACCTCGACT+AGG | + | Chr8:83556087-83556106 | MsG0880047224.01.T01:CDS | 45.0% | |
| ! | GGGAAGAGGCAAGGAATTTT+TGG | + | Chr8:83556127-83556146 | MsG0880047224.01.T01:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 83556075 | 83556326 | 83556075 | ID=MsG0880047224.01;Name=MsG0880047224.01 |
| Chr8 | mRNA | 83556075 | 83556326 | 83556075 | ID=MsG0880047224.01.T01;Parent=MsG0880047224.01;Name=MsG0880047224.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|83 |
| Chr8 | exon | 83556075 | 83556326 | 83556075 | ID=MsG0880047224.01.T01:exon:20688;Parent=MsG0880047224.01.T01 |
| Chr8 | CDS | 83556075 | 83556326 | 83556075 | ID=MsG0880047224.01.T01:cds;Parent=MsG0880047224.01.T01 |
| Gene Sequence |
| Protein sequence |
