Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047736.01.T01 | AES92794.2 | 88.563 | 341 | 18 | 4 | 1 | 334 | 1 | 327 | 0 | 599 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047736.01.T01 | MTR_4g133920 | 88.563 | 341 | 18 | 4 | 1 | 334 | 1 | 327 | 0.0 | 599 |
| MsG0880047736.01.T01 | MTR_3g115500 | 68.634 | 322 | 93 | 2 | 21 | 334 | 22 | 343 | 5.35e-157 | 444 |
| MsG0880047736.01.T01 | MTR_3g115500 | 68.634 | 322 | 93 | 2 | 21 | 334 | 40 | 361 | 1.31e-156 | 443 |
| MsG0880047736.01.T01 | MTR_1g020060 | 66.559 | 311 | 103 | 1 | 19 | 328 | 21 | 331 | 4.81e-150 | 425 |
| MsG0880047736.01.T01 | MTR_5g092120 | 56.875 | 320 | 131 | 3 | 8 | 323 | 32 | 348 | 7.76e-127 | 368 |
| MsG0880047736.01.T01 | MTR_1g117060 | 60.201 | 299 | 116 | 2 | 33 | 328 | 81 | 379 | 3.64e-126 | 370 |
| MsG0880047736.01.T01 | MTR_2g019990 | 61.204 | 299 | 113 | 2 | 33 | 328 | 73 | 371 | 3.20e-125 | 369 |
| MsG0880047736.01.T01 | MTR_3g115500 | 70.850 | 247 | 66 | 1 | 94 | 334 | 1 | 247 | 3.43e-125 | 359 |
| MsG0880047736.01.T01 | MTR_7g100500 | 55.901 | 322 | 139 | 2 | 5 | 323 | 29 | 350 | 4.91e-125 | 363 |
| MsG0880047736.01.T01 | MTR_1g067140 | 57.705 | 305 | 126 | 2 | 33 | 334 | 58 | 362 | 3.99e-124 | 361 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047736.01.T01 | AT1G20650 | 62.305 | 321 | 116 | 2 | 16 | 334 | 48 | 365 | 4.95e-140 | 402 |
| MsG0880047736.01.T01 | AT1G76370 | 60.681 | 323 | 120 | 3 | 13 | 331 | 42 | 361 | 4.04e-138 | 397 |
| MsG0880047736.01.T01 | AT5G18610 | 62.207 | 299 | 110 | 2 | 33 | 328 | 67 | 365 | 2.93e-128 | 377 |
| MsG0880047736.01.T01 | AT5G18610 | 62.207 | 299 | 110 | 2 | 33 | 328 | 67 | 365 | 2.93e-128 | 377 |
| MsG0880047736.01.T01 | AT5G18610 | 62.207 | 299 | 110 | 2 | 33 | 328 | 67 | 365 | 2.93e-128 | 377 |
| MsG0880047736.01.T01 | AT5G02800 | 60.473 | 296 | 114 | 2 | 36 | 328 | 60 | 355 | 5.89e-128 | 371 |
| MsG0880047736.01.T01 | AT3G24790 | 60.690 | 290 | 111 | 2 | 37 | 323 | 35 | 324 | 7.96e-125 | 362 |
| MsG0880047736.01.T01 | AT3G24790 | 60.690 | 290 | 111 | 2 | 37 | 323 | 53 | 342 | 1.71e-124 | 362 |
| MsG0880047736.01.T01 | AT3G07070 | 58.923 | 297 | 119 | 2 | 30 | 323 | 60 | 356 | 6.69e-123 | 359 |
| MsG0880047736.01.T01 | AT5G13160 | 57.895 | 304 | 125 | 2 | 28 | 328 | 65 | 368 | 1.09e-121 | 358 |
Find 82 sgRNAs with CRISPR-Local
Find 108 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATTTCAAGGAATTGCTA+GGG | 0.495721 | 8:-90227868 | MsG0880047736.01.T01:CDS |
| AAAGCAGACGACGTAGGCCT+CGG | 0.486267 | 8:+90226596 | None:intergenic |
| AAATTTCAAGGAATTGCTAG+GGG | 0.668281 | 8:-90227867 | MsG0880047736.01.T01:CDS |
| AACTTAATGGAGCTTTGTCT+TGG | 0.383635 | 8:+90227328 | None:intergenic |
| AAGAAATTAATATGTTTAGA+CGG | 0.552556 | 8:-90227971 | MsG0880047736.01.T01:CDS |
| AAGACAAAGCTCCATTAAGT+TGG | 0.511077 | 8:-90227326 | MsG0880047736.01.T01:CDS |
| AAGGAATTGCTAGGGGTAGG+TGG | 0.557168 | 8:-90227860 | MsG0880047736.01.T01:CDS |
| AAGTCTCGATGGATAACCGG+TGG | 0.642562 | 8:+90227237 | None:intergenic |
| AAGTTTGAGAGTGTGAGTAG+TGG | 0.655844 | 8:-90227947 | MsG0880047736.01.T01:CDS |
| AATAATTGATATGTGTCTCC+GGG | 0.527685 | 8:-90226572 | MsG0880047736.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAATTTCAAGGAATTGCTAG+GGG | - | Chr8:90226620-90226639 | MsG0880047736.01.T01:CDS | 30.0% | |
| AATAATTGATATGTGTCTCC+GGG | - | Chr8:90227915-90227934 | MsG0880047736.01.T01:CDS | 30.0% | |
| AGAGCAATACTTAGTTTCAT+GGG | - | Chr8:90227522-90227541 | MsG0880047736.01.T01:CDS | 30.0% | |
| GGATTTGGAAATGTTTACAA+GGG | - | Chr8:90226648-90226667 | MsG0880047736.01.T01:CDS | 30.0% | |
| GTTTATGAATACATGCCTAA+GGG | - | Chr8:90226977-90226996 | MsG0880047736.01.T01:CDS | 30.0% | |
| TGGATTTGGAAATGTTTACA+AGG | - | Chr8:90226647-90226666 | MsG0880047736.01.T01:CDS | 30.0% | |
| TTAATTGGTTATTGCACCAA+TGG | - | Chr8:90226938-90226957 | MsG0880047736.01.T01:intron | 30.0% | |
| AACTGCGACCAAAAATTTCA+AGG | - | Chr8:90226608-90226627 | MsG0880047736.01.T01:CDS | 35.0% | |
| AAGACAAAGCTCCATTAAGT+TGG | - | Chr8:90227161-90227180 | MsG0880047736.01.T01:CDS | 35.0% | |
| ACCTTATGCATAAACTCTCT+CGG | + | Chr8:90227841-90227860 | None:intergenic | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 90226480 | 90228029 | 90226480 | ID=MsG0880047736.01;Name=MsG0880047736.01 |
| Chr8 | mRNA | 90226480 | 90228029 | 90226480 | ID=MsG0880047736.01.T01;Parent=MsG0880047736.01;Name=MsG0880047736.01.T01;_AED=0.12;_eAED=0.12;_QI=0|0|0|1|1|1|4|0|334 |
| Chr8 | exon | 90227820 | 90228029 | 90227820 | ID=MsG0880047736.01.T01:exon:16132;Parent=MsG0880047736.01.T01 |
| Chr8 | exon | 90227487 | 90227676 | 90227487 | ID=MsG0880047736.01.T01:exon:16131;Parent=MsG0880047736.01.T01 |
| Chr8 | exon | 90226966 | 90227357 | 90226966 | ID=MsG0880047736.01.T01:exon:16130;Parent=MsG0880047736.01.T01 |
| Chr8 | exon | 90226480 | 90226692 | 90226480 | ID=MsG0880047736.01.T01:exon:16129;Parent=MsG0880047736.01.T01 |
| Chr8 | CDS | 90227820 | 90228029 | 90227820 | ID=MsG0880047736.01.T01:cds;Parent=MsG0880047736.01.T01 |
| Chr8 | CDS | 90227487 | 90227676 | 90227487 | ID=MsG0880047736.01.T01:cds;Parent=MsG0880047736.01.T01 |
| Chr8 | CDS | 90226966 | 90227357 | 90226966 | ID=MsG0880047736.01.T01:cds;Parent=MsG0880047736.01.T01 |
| Chr8 | CDS | 90226480 | 90226692 | 90226480 | ID=MsG0880047736.01.T01:cds;Parent=MsG0880047736.01.T01 |
| Gene Sequence |
>MsG0880047736.01.T01 ATGATCTTAGCTATTGAATGTGCAGAGGGTGAAAGGAAGAAATTAATATGTTTAGACGGAAAGTTTGAGAGTGTGAGTAGTGGTCACAGCAGCACATCAACAACTAGTTTTAGTTTTGATGAGCTTGTAACTGCGACCAAAAATTTCAAGGAATTGCTAGGGGTAGGTGGATTTGGAAATGTTTACAAGGGTCGTCTCCCAAACGGCGAGCTTGTTGCGGTTAAACAATTAAATCCTGATGGTTGTCAAGGCTGTCACGAATTTATGACAGAGCTTGATATTCTAAGTGTGCTGCGTCATGCTAATCTTGTAAAGTTAATTGGTTATTGCACCAATGGAGATCAAATGCTTTTGGTTTATGAATACATGCCTAAGGGATCTCTTGAAGCTCATCTTTTTGATGTTACCCAAGACAAAGCTCCATTAAGTTGGAGCACTCGGATAAAGATTTCTCTTGGAGCAGCTCAAGGTCTTGAGTATCTCCACTGTCAAGTGGATCCACCGGTTATCCATCGAGACTTGAAATCTTCAAACATTTTACTTGAAAATGACTTCAGTGCAAAACTGTCAGATTTCGGCCTTGCTAAACTTGGACCTGTTGGAGACGACACACATGTTTCAACCCGAGTGATGGGTACCGAAGGCTATTGCGCACTAGAATATGCTATGACTGGCAAATTAACTAAACAATCCGATATCTACAGTTTTGGTGTGGTTTTGCTGGAACTGATCACCGGACGTAGAGCCCTGGATACTAGTAGGGCAGTAGGAGAGCAATACTTAGTTTCATGGTGTCTCCCATATTTGAATGATCCGAGAGAGTTTATGCATAAGGTTGACCCTCTTTTGCAAGGACACTTCCCCAACCGAGGCCTACGTCGTCTGCTTTTAATAATTGATATGTGTCTCCGGGAGAATCCAACAGAACGTCCAGCCATTGGTGAAATTGTTGATGCACTAAAATACTTATATTCTAAAGTCTACAAACATCGTGTGCGCAGCTAA |
| Protein sequence |
>MsG0880047736.01.T01 MILAIECAEGERKKLICLDGKFESVSSGHSSTSTTSFSFDELVTATKNFKELLGVGGFGNVYKGRLPNGELVAVKQLNPDGCQGCHEFMTELDILSVLRHANLVKLIGYCTNGDQMLLVYEYMPKGSLEAHLFDVTQDKAPLSWSTRIKISLGAAQGLEYLHCQVDPPVIHRDLKSSNILLENDFSAKLSDFGLAKLGPVGDDTHVSTRVMGTEGYCALEYAMTGKLTKQSDIYSFGVVLLELITGRRALDTSRAVGEQYLVSWCLPYLNDPREFMHKVDPLLQGHFPNRGLRRLLLIIDMCLRENPTERPAIGEIVDALKYLYSKVYKHRVRS* |
