AlfalfaGEDB | MsaG000081

M. sativa ssp. caerulea / MsaG000081

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000081	AFK49400.1	94.395	339	15	1	8	346	69	403	0.0	650

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000081	I3TA58	94.395	339	15	1	8	346	69	403	0.0	650

TFs/TRs:

Gene ID	Type	Classification
MsaG000081	TF	C2H2

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000081	MsaG000234	0.838453	5.242404e-57	1.949860e-54
MsaG000081	MsaG000364	0.802802	7.932211e-49	1.142905e-46
MsaG000081	MsaG000520	0.836393	1.757037e-56	6.137007e-54
MsaG000081	MsaG001281	0.841768	7.221292e-58	2.980750e-55
MsaG000081	MsaG001283	0.813526	4.220072e-51	7.861256e-49
MsaG000081	MsaG001875	0.830756	4.419905e-55	1.307101e-52
MsaG000081	MsaG002405	0.836193	1.973914e-56	6.852779e-54
MsaG000081	MsaG002484	0.856283	6.934686e-62	4.694173e-59
MsaG000081	MsaG002731	0.845636	6.739408e-59	3.153724e-56
MsaG000081	MsaG003089	0.822755	3.524319e-53	8.341072e-51
MsaG000081	MsaG003252	0.855278	1.358150e-61	8.862899e-59
MsaG000081	MsaG003292	0.803616	5.394103e-49	7.919372e-47
MsaG000081	MsaG003407	0.852895	6.565009e-61	3.935066e-58
MsaG000081	MsaG003743	0.832606	1.554297e-55	4.850260e-53
MsaG000081	MsaG003750	0.870188	3.584659e-66	4.186967e-63
MsaG000081	MsaG003769	0.863981	3.360769e-64	3.048683e-61
MsaG000081	MsaG004004	0.839962	2.138739e-57	8.339126e-55
MsaG000081	MsaG003934	0.820107	1.430637e-52	3.155520e-50
MsaG000081	MsaG003938	0.862970	6.890838e-64	6.007030e-61
MsaG000081	MsaG004227	0.869903	4.437737e-66	5.121872e-63
MsaG000081	MsaG004186	0.803302	6.259485e-49	9.123634e-47
MsaG000081	MsaG004367	0.827130	3.307868e-54	8.827646e-52
MsaG000081	MsaG004418	0.814177	3.037829e-51	5.752003e-49
MsaG000081	MsaG005023	0.811416	1.214058e-50	2.146252e-48
MsaG000081	MsaG005351	0.847372	2.275648e-59	1.128336e-56
MsaG000081	MsaG005598	0.850038	4.182614e-60	2.269711e-57
MsaG000081	MsaG005961	0.819360	2.115565e-52	4.575126e-50
MsaG000081	MsaG006053	0.833712	8.268422e-56	2.665405e-53
MsaG000081	MsaG006357	0.836088	2.098001e-56	7.260529e-54
MsaG000081	MsaG006425	0.852519	8.393553e-61	4.964799e-58
MsaG000081	MsaG006586	0.842334	5.124643e-58	2.153972e-55
MsaG000081	MsaG006743	0.880357	1.240223e-69	2.276052e-66
MsaG000081	MsaG006910	0.851234	1.935769e-60	1.094735e-57
MsaG000081	MsaG006925	0.832454	1.694509e-55	5.264461e-53
MsaG000081	MsaG007663	0.827941	2.117431e-54	5.780774e-52
MsaG000081	MsaG007665	0.877872	9.282910e-69	1.518785e-65
MsaG000081	MsaG007759	0.846815	3.228617e-59	1.571239e-56
MsaG000081	MsaG008168	0.831737	2.543138e-55	7.738742e-53
MsaG000081	MsaG009372	0.823950	1.858729e-53	4.543662e-51
MsaG000081	MsaG009864	0.835492	2.966492e-56	1.008421e-53
MsaG000081	MsaG009880	0.813820	3.637990e-51	6.827281e-49
MsaG000081	MsaG010418	0.856053	8.092347e-62	5.431392e-59
MsaG000081	MsaG010621	0.819968	1.539023e-52	3.382065e-50
MsaG000081	MsaG011133	0.826740	4.094628e-54	1.080928e-51
MsaG000081	MsaG011657	0.803330	6.176597e-49	9.008539e-47
MsaG000081	MsaG011702	0.812766	6.184556e-51	1.130352e-48
MsaG000081	MsaG011749	0.854824	1.837879e-61	1.179837e-58
MsaG000081	MsaG012458	0.833599	8.824423e-56	2.835181e-53
MsaG000081	MsaG012461	0.824281	1.555865e-53	3.837704e-51
MsaG000081	MsaG012521	0.817155	6.639722e-52	1.356060e-49
MsaG000081	MsaG012657	0.805183	2.551138e-49	3.884251e-47
MsaG000081	MsaG012747	0.820092	1.442189e-52	3.179695e-50
MsaG000081	MsaG012982	0.864158	2.961533e-64	2.705638e-61
MsaG000081	MsaG013708	0.842701	4.098365e-58	1.743115e-55
MsaG000081	MsaG013895	0.808936	4.133294e-50	6.879648e-48
MsaG000081	MsaG015329	0.813555	4.158036e-51	7.751362e-49
MsaG000081	MsaG015347	0.813862	3.562113e-51	6.691745e-49
MsaG000081	MsaG015358	0.820416	1.216232e-52	2.704631e-50
MsaG000081	MsaG016688	0.835649	2.708189e-56	9.249268e-54
MsaG000081	MsaG016827	0.823852	1.960094e-53	4.778643e-51
MsaG000081	MsaG016906	0.830866	4.155372e-55	1.232788e-52
MsaG000081	MsaG016972	0.822190	4.763767e-53	1.110348e-50
MsaG000081	MsaG017085	0.829898	7.148070e-55	2.062650e-52
MsaG000081	MsaG017418	0.833725	8.208156e-56	2.646975e-53
MsaG000081	MsaG017658	0.831076	3.693547e-55	1.102370e-52
MsaG000081	MsaG017802	0.810655	1.771042e-50	3.073120e-48
MsaG000081	MsaG018097	0.811782	1.011572e-50	1.804410e-48
MsaG000081	MsaG018226	0.814320	2.824779e-51	5.367859e-49
MsaG000081	MsaG018305	0.844826	1.113291e-58	5.072548e-56
MsaG000081	MsaG018726	0.847351	2.306958e-59	1.143020e-56
MsaG000081	MsaG018806	0.844502	1.359916e-58	6.129829e-56
MsaG000081	MsaG018863	0.836102	2.081217e-56	7.205565e-54
MsaG000081	MsaG018919	0.832794	1.396839e-55	4.382864e-53
MsaG000081	MsaG019031	0.841440	8.802180e-58	3.595788e-55
MsaG000081	MsaG019214	0.868521	1.241468e-65	1.353041e-62
MsaG000081	MsaG019492	0.816021	1.188777e-51	2.358204e-49
MsaG000081	MsaG019621	0.803324	6.194909e-49	9.034075e-47
MsaG000081	MsaG019623	0.803923	4.660443e-49	6.891193e-47
MsaG000081	MsaG019629	0.806506	1.349227e-49	2.119115e-47
MsaG000081	MsaG020016	0.874082	1.840407e-67	2.542253e-64
MsaG000081	MsaG020135	0.883329	1.052040e-70	2.222435e-67
MsaG000081	MsaG020312	0.863680	4.163522e-64	3.732598e-61
MsaG000081	MsaG020365	0.840567	1.488480e-57	5.915050e-55
MsaG000081	MsaG020370	0.809044	3.920257e-50	6.542109e-48
MsaG000081	MsaG020578	0.838813	4.235659e-57	1.593289e-54
MsaG000081	MsaG020670	0.856622	5.519247e-62	3.782387e-59
MsaG000081	MsaG021077	0.853365	4.822210e-61	2.938877e-58
MsaG000081	MsaG021079	0.813229	4.899274e-51	9.058996e-49
MsaG000081	MsaG022009	0.830029	6.641523e-55	1.923770e-52
MsaG000081	MsaG022557	0.836447	1.702135e-56	5.954722e-54
MsaG000081	MsaG023817	0.836295	1.859852e-56	6.476861e-54
MsaG000081	MsaG023907	0.910028	7.156312e-82	6.765202e-78
MsaG000081	MsaG023897	0.805454	2.240525e-49	3.432760e-47
MsaG000081	MsaG023993	0.840443	1.603988e-57	6.349228e-55
MsaG000081	MsaG024136	0.808571	4.941697e-50	8.153262e-48
MsaG000081	MsaG024152	0.812842	5.951200e-51	1.089791e-48
MsaG000081	MsaG024652	0.854000	3.173926e-61	1.978662e-58
MsaG000081	MsaG025081	0.821391	7.274543e-53	1.659913e-50
MsaG000081	MsaG025160	0.830340	5.580761e-55	1.630912e-52
MsaG000081	MsaG026848	0.835065	3.798267e-56	1.274768e-53
MsaG000081	MsaG026926	0.813359	4.589728e-51	8.514025e-49
MsaG000081	MsaG026966	0.858639	1.403418e-62	1.036346e-59
MsaG000081	MsaG027054	0.801333	1.585116e-48	2.208708e-46
MsaG000081	MsaG027424	0.810544	1.871883e-50	3.239328e-48
MsaG000081	MsaG027816	0.802883	7.634721e-49	1.102101e-46
MsaG000081	MsaG027830	0.802881	7.643961e-49	1.103359e-46
MsaG000081	MsaG028607	0.809966	2.490829e-50	4.250010e-48
MsaG000081	MsaG028950	0.847804	1.733487e-59	8.721863e-57
MsaG000081	MsaG028985	0.840157	1.902911e-57	7.465115e-55
MsaG000081	MsaG029236	0.863292	5.485353e-64	4.842631e-61
MsaG000081	MsaG029813	0.812178	8.300758e-51	1.495130e-48
MsaG000081	MsaG030518	0.817935	4.438533e-52	9.248772e-50
MsaG000081	MsaG031491	0.802506	9.124038e-49	1.305790e-46
MsaG000081	MsaG032378	0.802769	8.058031e-49	1.160147e-46
MsaG000081	MsaG033966	0.852296	9.707343e-61	5.698344e-58
MsaG000081	MsaG034790	0.828281	1.755298e-54	4.837954e-52
MsaG000081	MsaG034949	0.825927	6.379654e-54	1.646568e-51
MsaG000081	MsaG034999	0.827992	2.057941e-54	5.626542e-52
MsaG000081	MsaG035269	0.836136	2.041084e-56	7.073827e-54
MsaG000081	MsaG035275	0.814337	2.801068e-51	5.325158e-49
MsaG000081	MsaG035323	0.856138	7.642602e-62	5.145522e-59
MsaG000081	MsaG035558	0.809659	2.897200e-50	4.907025e-48
MsaG000081	MsaG036060	0.826317	5.159194e-54	1.345988e-51
MsaG000081	MsaG036111	0.860202	4.785818e-63	3.750157e-60
MsaG000081	MsaG036130	0.869833	4.678464e-66	5.383250e-63
MsaG000081	MsaG036131	0.872165	8.032767e-67	1.020844e-63
MsaG000081	MsaG036699	0.825135	9.812002e-54	2.477355e-51
MsaG000081	MsaG036701	0.814122	3.122503e-51	5.904281e-49
MsaG000081	MsaG038022	0.800441	2.406990e-48	3.286986e-46
MsaG000081	MsaG039591	0.833995	7.033107e-56	2.285984e-53
MsaG000081	MsaG040755	0.832757	1.426600e-55	4.471461e-53
MsaG000081	MsaG041015	0.846333	4.366850e-59	2.091251e-56
MsaG000081	MsaG041017	0.865351	1.257805e-64	1.205174e-61
MsaG000081	MsaG041018	0.811262	1.310455e-50	2.307987e-48
MsaG000081	MsaG041019	0.868319	1.441029e-65	1.557556e-62
MsaG000081	MsaG041044	0.836172	1.998125e-56	6.932543e-54
MsaG000081	MsaG041045	0.800559	2.277474e-48	3.118443e-46
MsaG000081	MsaG041172	0.855136	1.493565e-61	9.696074e-59
MsaG000081	MsaG041230	0.831186	3.470217e-55	1.039014e-52
MsaG000081	MsaG041392	0.858016	2.147281e-62	1.549317e-59
MsaG000081	MsaG042225	0.839749	2.428711e-57	9.406652e-55
MsaG000081	MsaG042323	0.816108	1.136946e-51	2.260383e-49
MsaG000081	MsaG043344	0.813803	3.668570e-51	6.881809e-49
MsaG000081	MsaG044324	0.828889	1.253440e-54	3.514281e-52
MsaG000081	MsaG045283	0.801511	1.458420e-48	2.040328e-46
MsaG000081	MsaG045550	0.861633	1.766660e-63	1.461637e-60
MsaG000081	MsaG045551	0.860216	4.739471e-63	3.715871e-60
MsaG000081	MsaG045766	0.818245	3.780148e-52	7.939940e-50
MsaG000081	MsaG045806	0.806798	1.171553e-49	1.852712e-47
MsaG000081	MsaG046276	0.804472	3.585614e-49	5.369813e-47
MsaG000081	MsaG046602	0.818007	4.275565e-52	8.926045e-50
MsaG000081	MsaG046609	0.884451	4.074749e-71	9.085861e-68
MsaG000081	MsaG046766	0.804728	3.173306e-49	4.780553e-47
MsaG000081	MsaG046804	0.923646	5.279029e-89	1.304525e-84
MsaG000081	MsaG046955	0.852581	8.059347e-61	4.777876e-58
MsaG000081	MsaG002505	0.836508	1.642904e-56	5.757974e-54
MsaG000081	MsaG003316	0.830648	4.697702e-55	1.384864e-52
MsaG000081	MsaG004869	0.813016	5.453982e-51	1.003098e-48
MsaG000081	MsaG001683	0.816863	7.717008e-52	1.564200e-49
MsaG000081	MsaG002115	0.805715	1.975727e-49	3.045613e-47
MsaG000081	MsaG002551	0.833926	7.316961e-56	2.373427e-53
MsaG000081	MsaG011523	0.806129	1.618531e-49	2.519464e-47
MsaG000081	MsaG014171	0.819320	2.159960e-52	4.666243e-50
MsaG000081	MsaG013507	0.824226	1.602690e-53	3.947171e-51
MsaG000081	MsaG013255	0.803549	5.566469e-49	8.160218e-47
MsaG000081	MsaG019459	0.879127	3.375577e-69	5.852751e-66
MsaG000081	MsaG019026	0.832163	1.998538e-55	6.157104e-53
MsaG000081	MsaG022732	0.803726	5.117569e-49	7.532860e-47
MsaG000081	MsaG025840	0.867560	2.520905e-65	2.641190e-62
MsaG000081	MsaG026810	0.819145	2.366829e-52	5.089722e-50
MsaG000081	MsaG032143	0.841003	1.145750e-57	4.615895e-55
MsaG000081	MsaG030927	0.834160	6.395933e-56	2.089176e-53
MsaG000081	MsaG031458	0.820871	9.572816e-53	2.154463e-50
MsaG000081	MsaG031111	0.831537	2.848178e-55	8.615664e-53
MsaG000081	MsaG031373	0.848932	8.480704e-60	4.431951e-57
MsaG000081	MsaG032719	0.854538	2.223050e-61	1.412680e-58
MsaG000081	MsaG030868	0.807036	1.043768e-49	1.659897e-47
MsaG000081	MsaG037255	0.867319	3.007396e-65	3.119893e-62
MsaG000081	MsaG037000	0.839960	2.140371e-57	8.345106e-55
MsaG000081	MsaG037256	0.838411	5.374684e-57	1.996372e-54
MsaG000081	MsaG037532	0.835774	2.518300e-56	8.633080e-54
MsaG000081	MsaG036656	0.857427	3.202820e-62	2.260810e-59

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000081	MtrunA17_Chr1g0156521	94.395	339	15	1	8	346	69	403	0.0	650
MsaG000081	MtrunA17_Chr3g0114991	58.759	274	90	7	87	343	183	450	6.84e-107	320
MsaG000081	MtrunA17_Chr4g0042091	51.667	240	103	3	114	343	200	436	1.54e-71	229
MsaG000081	MtrunA17_Chr3g0104431	43.396	265	123	10	99	344	133	389	5.07e-59	195
MsaG000081	MtrunA17_Chr2g0280501	44.726	237	114	6	119	343	145	376	2.86e-54	182
MsaG000081	MtrunA17_Chr5g0399041	40.097	207	100	7	141	344	105	290	1.59e-37	136
MsaG000081	MtrunA17_Chr4g0056461	40.000	205	92	6	144	344	98	275	3.87e-37	134
MsaG000081	MtrunA17_Chr8g0376191	39.181	171	89	4	183	344	2	166	4.69e-32	118
MsaG000081	MtrunA17_Chr2g0280521	48.113	106	55	0	148	253	10	115	6.89e-26	100
MsaG000081	MtrunA17_Chr5g0445321	32.124	193	114	7	157	342	1	183	3.62e-25	100

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000081	AT1G75710.1	60.743	377	114	8	1	345	86	460	7.24e-142	410
MsaG000081	AT4G27240.1	41.484	364	161	10	15	343	84	430	6.22e-71	227
MsaG000081	AT5G54630.1	49.793	241	103	4	119	343	233	471	2.69e-69	224
MsaG000081	AT4G27240.2	40.533	338	149	10	15	317	84	404	1.13e-58	196
MsaG000081	AT2G29660.1	42.169	249	118	7	119	344	128	373	3.80e-53	179
MsaG000081	AT1G11490.2	39.095	243	123	7	119	343	129	364	8.08e-42	149
MsaG000081	AT1G11490.1	39.095	243	123	7	119	343	129	364	8.08e-42	149
MsaG000081	AT4G12450.1	35.160	219	100	5	136	344	91	277	5.38e-32	121
MsaG000081	AT4G22560.1	37.981	208	90	6	141	344	92	264	6.86e-30	115
MsaG000081	AT1G62520.1	36.585	205	104	7	142	344	100	280	2.10e-29	114

Find 89 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGTTCCAAGCTTTCAATCTT+AGG	0.165279	1:+1107096	None:intergenic
ACTCGCTCTGCATCATGTTC+TGG	0.262275	1:-1107878	MsaT000081.1:CDS
CAACGACATAATGTGCTTCT+AGG	0.275334	1:+1107141	None:intergenic
CTTCAAGAGGAATGCAGTTT+AGG	0.296779	1:-1107814	MsaT000081.1:CDS
TCTGCCCTTTGCGCTGTGCC+TGG	0.333857	1:-1104674	MsaT000081.1:CDS
CTGGTGTGCCGCGTGATTGC+CGG	0.335086	1:-1104533	MsaT000081.1:CDS
AGCTTCAGAGATGTTGTTCA+TGG	0.341820	1:-1108016	MsaT000081.1:CDS
CATAATGTGCTTCTAGGAAT+TGG	0.382545	1:+1107147	None:intergenic
GATTCTATTGCGGAGAATGC+TGG	0.385728	1:-1104446	MsaT000081.1:CDS
ATCACCAGAGAGTAGTATCT+TGG	0.393830	1:-1107960	MsaT000081.1:CDS
CGCCACGCGCTTCACTTTCC+CGG	0.395766	1:+1104514	None:intergenic
CAGTTGGAGAAGATTCTAGA+AGG	0.406367	1:-1104562	MsaT000081.1:CDS
ATGCAGTTTAGGAAGCTATC+TGG	0.408827	1:-1107803	MsaT000081.1:CDS
CTCGCTCTGCATCATGTTCT+GGG	0.411037	1:-1107877	MsaT000081.1:CDS
GTTCCAAGCTTTCAATCTTA+GGG	0.412870	1:+1107097	None:intergenic
CTTCCCTAAGATTGAAAGCT+TGG	0.415664	1:-1107100	MsaT000081.1:CDS
GAGCTGGGTCCAGAGGATTC+AGG	0.426596	1:-1104947	MsaT000081.1:CDS
TTGTTGAACAGTTTCGGAGC+TGG	0.439362	1:-1104963	MsaT000081.1:intron
ATGTTTCTGCCTGAATCCTC+TGG	0.441608	1:+1104938	None:intergenic
TGTTGAACAGTTTCGGAGCT+GGG	0.442643	1:-1104962	MsaT000081.1:intron
CTCTGCATTCCTCGAACCGT+TGG	0.442678	1:+1104819	None:intergenic
CTTTGCGCTGTGCCTGGATG+CGG	0.457029	1:-1104668	MsaT000081.1:CDS
GCGGAGTTTGCAACATCATC+CGG	0.466040	1:-1104649	MsaT000081.1:CDS
AAACATTATGTTAACCTGCT+AGG	0.471824	1:+1107752	None:intergenic
AACATCTCTGAAGCTACAAA+TGG	0.481428	1:+1108024	None:intergenic
GATGGTGTCAGAGGAGGAAA+TGG	0.486566	1:-1104477	MsaT000081.1:CDS
CGCATCCAGGCACAGCGCAA+AGG	0.489351	1:+1104669	None:intergenic
TCACCAGAGAGTAGTATCTT+GGG	0.490800	1:-1107959	MsaT000081.1:CDS
AGGCACAGCGCAAAGGGCAG+AGG	0.492922	1:+1104676	None:intergenic
ACGAACAATCCAACGGTTCG+AGG	0.494391	1:-1104828	MsaT000081.1:CDS
CTTGGTGGTGTTGGATGCAC+GGG	0.495904	1:+1104784	None:intergenic
AACATTATGTTAACCTGCTA+GGG	0.496126	1:+1107753	None:intergenic
GAAAGTGAAGCGCGTGGCGG+AGG	0.498808	1:-1104510	MsaT000081.1:CDS
TCTTGGTGGTGTTGGATGCA+CGG	0.499082	1:+1104783	None:intergenic
GCATCCAGGCACAGCGCAAA+GGG	0.499268	1:+1104670	None:intergenic
ACATGATCGTTGACCCTAGC+AGG	0.499380	1:-1107766	MsaT000081.1:intron
ATTCTATTGCGGAGAATGCT+GGG	0.506191	1:-1104445	MsaT000081.1:CDS
TGGTGTGCCGCGTGATTGCC+GGG	0.506413	1:-1104532	MsaT000081.1:CDS
CATCAAGCTGTTCGACATGC+AGG	0.509515	1:-1107072	MsaT000081.1:intron
AGATTCTAGAAGGGCAATGC+TGG	0.511299	1:-1104552	MsaT000081.1:CDS
ATAGCGATGTGCTGTGGAAA+CGG	0.513165	1:+1104723	None:intergenic
GAAACACTAGAGTTGTTCAT+AGG	0.519636	1:-1107994	MsaT000081.1:CDS
AAACAAGGAAGAATAGCTCT+TGG	0.523135	1:+1104386	None:intergenic
CTTCTCCAACTGAATTGTCA+TGG	0.528807	1:+1104573	None:intergenic
CTACATCTTGACTAATACGT+GGG	0.532590	1:+1110229	None:intergenic
GGCGGAGGAAGGTGCACCGA+TGG	0.536381	1:-1104495	MsaT000081.1:CDS
GTGAAGCGCGTGGCGGAGGA+AGG	0.538219	1:-1104506	MsaT000081.1:CDS
GGATTGTTCGTTGTGTGTTG+TGG	0.544405	1:+1104840	None:intergenic
TTCTCCAACTGAATTGTCAT+GGG	0.545184	1:+1104574	None:intergenic
ATGTTGCAAACTCCGCATCC+AGG	0.550457	1:+1104656	None:intergenic
GATGATGATGAAGCAGTGAC+AGG	0.553941	1:+1107908	None:intergenic
GGGAAATCATCCAACGGTGG+AGG	0.554162	1:-1107857	MsaT000081.1:CDS
AGATAATCTTCAAATCTAGC+TGG	0.554427	1:-1104910	MsaT000081.1:CDS
GGCCCTACCACTACTAGCAT+TGG	0.555926	1:+1104595	None:intergenic
AGGGCCCATGACAATTCAGT+TGG	0.558945	1:-1104578	MsaT000081.1:CDS
AATCCACGGTGTGCCGCGGA+CGG	0.561977	1:-1104758	MsaT000081.1:CDS
TGTGTAGTTTCCTCCACCGT+TGG	0.563416	1:+1107847	None:intergenic
TGACCCAAGATACTACTCTC+TGG	0.570001	1:+1107956	None:intergenic
GAGGAGTTCGTTGCCGTCCG+CGG	0.571453	1:+1104745	None:intergenic
GACCAATGCTAGTAGTGGTA+GGG	0.575088	1:-1104597	MsaT000081.1:CDS
CTCCAATGTGCAAGATTGAA+CGG	0.576390	1:-1104874	MsaT000081.1:CDS
AGACCAATGCTAGTAGTGGT+AGG	0.580999	1:-1104598	MsaT000081.1:CDS
TGCAAGGTATTCGGTCAACA+TGG	0.581335	1:+1104341	None:intergenic
ATCCGTTCAATCTTGCACAT+TGG	0.586137	1:+1104872	None:intergenic
TTGCCGTCCGCGGCACACCG+TGG	0.592005	1:+1104755	None:intergenic
AGTTTCGGAGCTGGGTCCAG+AGG	0.594982	1:-1104954	MsaT000081.1:CDS
TCGTGTGCACTCGGTGCAAG+TGG	0.597474	1:-1104701	MsaT000081.1:CDS
TCACTTTCCCGGCAATCACG+CGG	0.600425	1:+1104525	None:intergenic
ATTTCCTCCTCTGACACCAT+CGG	0.601854	1:+1104479	None:intergenic
ACATCGCTATCGTGTGCACT+CGG	0.604788	1:-1104710	MsaT000081.1:CDS
AGTTGGAGAAGATTCTAGAA+GGG	0.607565	1:-1104561	MsaT000081.1:CDS
AGGTGCACCGATGGTGTCAG+AGG	0.618774	1:-1104486	MsaT000081.1:CDS
ACATCATCATCATCTTCAAG+AGG	0.620776	1:-1107827	MsaT000081.1:CDS
TGCACCGATGGTGTCAGAGG+AGG	0.621271	1:-1104483	MsaT000081.1:CDS
GTGAAGACCAATGCTAGTAG+TGG	0.632554	1:-1104602	MsaT000081.1:CDS
TTGTAAATAACTACGAAACA+AGG	0.649310	1:+1104371	None:intergenic
CGGGAAAGTGAAGCGCGTGG+CGG	0.664636	1:-1104513	MsaT000081.1:CDS
GTTTGCAACATCATCCGGCA+CGG	0.666115	1:-1104644	MsaT000081.1:CDS
TGTTCTGGGAAATCATCCAA+CGG	0.668023	1:-1107863	MsaT000081.1:CDS
TCTACATCTTGACTAATACG+TGG	0.669784	1:+1110228	None:intergenic
TGCCGGGAAAGTGAAGCGCG+TGG	0.670291	1:-1104516	MsaT000081.1:CDS
CACACGATAGCGATGTGCTG+TGG	0.671968	1:+1104717	None:intergenic
GCGATGTGCTGTGGAAACGG+AGG	0.680201	1:+1104726	None:intergenic
TACATCTTGACTAATACGTG+GGG	0.691051	1:+1110230	None:intergenic
ACACACAACGAACAATCCAA+CGG	0.693116	1:-1104835	MsaT000081.1:CDS
TTATGTCGTTGTGATAAGTG+TGG	0.706591	1:-1107129	MsaT000081.1:CDS
GTTCGAGGAATGCAGAGACG+CGG	0.728390	1:-1104813	MsaT000081.1:CDS
TCGTTGTGATAAGTGTGGAG+AGG	0.731697	1:-1107124	MsaT000081.1:CDS
TCTGGGAAATCATCCAACGG+TGG	0.734107	1:-1107860	MsaT000081.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	1104355	1110268	1104355	ID=MsaG000081
Chr1	mRNA	1104355	1110268	1104355	ID=MsaT000081.1;Parent=MsaG000081
Chr1	exon	1104355	1104974	1104355	ID=MsaT000081.1.exon4;Parent=MsaT000081.1
Chr1	CDS	1104355	1104974	1104355	ID=cds.MsaT000081.1;Parent=MsaT000081.1
Chr1	exon	1107073	1107173	1107073	ID=MsaT000081.1.exon3;Parent=MsaT000081.1
Chr1	CDS	1107073	1107173	1107073	ID=cds.MsaT000081.1;Parent=MsaT000081.1
Chr1	exon	1107767	1108048	1107767	ID=MsaT000081.1.exon2;Parent=MsaT000081.1
Chr1	CDS	1107767	1108048	1107767	ID=cds.MsaT000081.1;Parent=MsaT000081.1
Chr1	exon	1110228	1110268	1110228	ID=MsaT000081.1.exon1;Parent=MsaT000081.1
Chr1	CDS	1110228	1110268	1110228	ID=cds.MsaT000081.1;Parent=MsaT000081.1

Gene Sequence

>MsaT000081.1

ATGACTCATAAAGACTCCCCACGTATTAGTCAAGATGTAGACTCCATTTGTAGCTTCAGAGATGTTGTTCATGGAAACACTAGAGTTGTTCATAGGTCTGATAATTCATCACCAGAGAGTAGTATCTTGGGTCAAGAAACAAGTTTACTCAAAAGAAAACCTGTCACTGCTTCATCATCATCAACATCAACTCGCTCTGCATCATGTTCTGGGAAATCATCCAACGGTGGAGGAAACTACACATCATCATCATCTTCAAGAGGAATGCAGTTTAGGAAGCTATCTGGATGTTATGAATGTCACATGATCGTTGACCCTAGCAGGCTACCAATTCCTAGAAGCACATTATGTCGTTGTGATAAGTGTGGAGAGGTCTTCCCTAAGATTGAAAGCTTGGAACTTCATCAAGCTGTTCGACATGCAGTTTCGGAGCTGGGTCCAGAGGATTCAGGCAGAAACATAGTAGAGATAATCTTCAAATCTAGCTGGTTAAAGAGAGAGACTCCAATGTGCAAGATTGAACGGATACTAAAAGTCCACAACACACAACGAACAATCCAACGGTTCGAGGAATGCAGAGACGCGGTAAAATCCCGTGCATCCAACACCACCAAGAAAAATCCACGGTGTGCCGCGGACGGCAACGAACTCCTCCGTTTCCACAGCACATCGCTATCGTGTGCACTCGGTGCAAGTGGTTCCTCTGCCCTTTGCGCTGTGCCTGGATGCGGAGTTTGCAACATCATCCGGCACGGTTTTCAAAAGAAAGGTGGAGTGAAGACCAATGCTAGTAGTGGTAGGGCCCATGACAATTCAGTTGGAGAAGATTCTAGAAGGGCAATGCTGGTGTGCCGCGTGATTGCCGGGAAAGTGAAGCGCGTGGCGGAGGAAGGTGCACCGATGGTGTCAGAGGAGGAAATGGTGTGTTTTGATTCTATTGCGGAGAATGCTGGGAGTTACTCTAATCTCGAAGATTTAACTGTTTTCAATCCAAGAGCTATTCTTCCTTGTTTCGTAGTTATTTACAAAGTGCTTCCATGTTGA

Protein sequence

>MsaT000081.1

MTHKDSPRISQDVDSICSFRDVVHGNTRVVHRSDNSSPESSILGQETSLLKRKPVTASSSSTSTRSASCSGKSSNGGGNYTSSSSSRGMQFRKLSGCYECHMIVDPSRLPIPRSTLCRCDKCGEVFPKIESLELHQAVRHAVSELGPEDSGRNIVEIIFKSSWLKRETPMCKIERILKVHNTQRTIQRFEECRDAVKSRASNTTKKNPRCAADGNELLRFHSTSLSCALGASGSSALCAVPGCGVCNIIRHGFQKKGGVKTNASSGRAHDNSVGEDSRRAMLVCRVIAGKVKRVAEEGAPMVSEEEMVCFDSIAENAGSYSNLEDLTVFNPRAILPCFVVIYKVLPC*