AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG000100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000100 MtrunA17_Chr1g0156321 94.786 863 39 3 1 860 1 860 0.0 1682
MsaG000100 MtrunA17_Chr1g0156301 87.633 283 19 1 578 860 1 267 9.28e-175 505
MsaG000100 MtrunA17_Chr2g0285461 65.940 367 118 4 35 395 15 380 4.52e-160 490
MsaG000100 MtrunA17_Chr5g0433301 66.213 367 117 4 35 395 16 381 6.89e-160 488
MsaG000100 MtrunA17_Chr5g0433301 41.060 151 71 3 727 859 670 820 7.52e-26 114
MsaG000100 MtrunA17_Chr4g0070181 70.845 343 94 4 35 373 15 355 2.25e-159 494
MsaG000100 MtrunA17_Chr4g0070181 39.631 217 120 4 631 846 880 1086 5.82e-34 140
MsaG000100 MtrunA17_Chr3g0109391 66.301 365 116 4 37 395 20 383 5.54e-159 486
MsaG000100 MtrunA17_Chr3g0109391 42.763 152 84 2 709 859 679 828 2.51e-27 119
MsaG000100 MtrunA17_Chr2g0301251 69.096 343 100 4 35 373 15 355 1.66e-155 484
MsaG000100 MtrunA17_Chr2g0301251 60.952 105 41 0 742 846 974 1078 8.00e-33 137
MsaG000100 MtrunA17_Chr8g0376771 64.850 367 121 5 35 395 15 379 1.24e-152 471
MsaG000100 MtrunA17_Chr8g0376771 50.806 124 61 0 736 859 763 886 6.15e-31 130
MsaG000100 MtrunA17_Chr8g0389081 65.976 338 111 2 40 373 29 366 1.65e-141 447
MsaG000100 MtrunA17_Chr8g0389081 39.806 206 100 6 646 846 891 1077 4.02e-34 141
MsaG000100 MtrunA17_Chr8g0385791 51.917 339 157 4 39 373 55 391 3.45e-111 360
MsaG000100 MtrunA17_Chr8g0385791 42.857 91 50 2 754 843 710 799 4.02e-15 80.1
MsaG000100 MtrunA17_Chr2g0276781 52.071 338 157 3 39 373 19 354 6.44e-111 354
MsaG000100 MtrunA17_Chr2g0276781 48.387 93 44 3 753 843 546 636 7.96e-18 88.6
MsaG000100 MtrunA17_Chr8g0346711 49.280 347 168 5 31 373 16 358 6.67e-104 336
MsaG000100 MtrunA17_Chr8g0346711 41.593 113 57 4 744 852 545 652 9.86e-16 81.6
MsaG000100 MtrunA17_Chr2g0326281 48.485 363 158 7 43 378 56 416 2.43e-101 333
MsaG000100 MtrunA17_Chr2g0326281 42.308 104 58 2 754 856 695 797 4.17e-18 89.7
MsaG000100 MtrunA17_Chr2g0282961 48.991 347 149 6 43 373 53 387 9.45e-101 326
MsaG000100 MtrunA17_Chr4g0046371 46.757 370 162 8 43 390 38 394 1.12e-99 325
MsaG000100 MtrunA17_Chr4g0029671 48.276 348 158 6 43 373 54 396 1.20e-99 328
MsaG000100 MtrunA17_Chr4g0029671 45.055 91 48 2 754 843 663 752 2.08e-18 90.5
MsaG000100 MtrunA17_Chr4g0009751 49.123 342 156 8 41 373 17 349 4.30e-96 313
MsaG000100 MtrunA17_Chr7g0239061 45.640 344 177 6 35 373 6 344 1.02e-92 306
MsaG000100 MtrunA17_Chr7g0239061 42.857 91 49 2 754 843 570 658 1.11e-14 78.2
MsaG000100 MtrunA17_Chr1g0181211 36.979 384 192 6 36 373 20 399 9.47e-74 255
MsaG000100 MtrunA17_Chr2g0326761 39.467 375 183 8 38 373 6 375 1.00e-73 255
MsaG000100 MtrunA17_Chr4g0028971 39.370 381 177 8 40 373 9 382 1.29e-71 249
MsaG000100 MtrunA17_Chr1g0199681 36.962 395 202 8 38 391 89 477 1.76e-71 249
MsaG000100 MtrunA17_Chr7g0264171 34.719 409 195 10 36 382 24 422 1.96e-66 233
MsaG000100 MtrunA17_Chr3g0115271 36.198 384 204 7 39 384 15 395 1.31e-61 219
MsaG000100 MtrunA17_Chr7g0264161 37.097 310 178 6 52 353 1 301 7.96e-61 208
MsaG000100 MtrunA17_Chr8g0356211 42.667 225 121 4 151 373 2 220 3.11e-54 190
MsaG000100 MtrunA17_Chr5g0417281 32.184 435 245 16 37 454 5 406 1.88e-52 191
MsaG000100 MtrunA17_Chr5g0424931 33.333 369 198 10 38 373 14 367 6.26e-51 187
MsaG000100 MtrunA17_Chr5g0436761 33.509 379 211 15 38 391 7 369 9.11e-48 176
MsaG000100 MtrunA17_Chr1g0178011 31.608 367 220 11 38 391 7 355 1.45e-44 167
MsaG000100 MtrunA17_Chr2g0289221 34.451 328 172 14 43 353 20 321 5.18e-40 151
MsaG000100 MtrunA17_Chr2g0277461 31.953 338 209 10 43 373 13 336 2.02e-38 149
MsaG000100 MtrunA17_Chr5g0417211 31.611 329 185 12 40 352 9 313 4.19e-36 141
MsaG000100 MtrunA17_Chr5g0424741 30.982 397 213 16 38 393 7 383 1.49e-35 142
MsaG000100 MtrunA17_Chr1g0156311 61.475 122 14 3 261 382 1 89 8.18e-33 122
MsaG000100 MtrunA17_Chr2g0277531 28.923 325 197 12 40 353 17 318 1.59e-31 125
MsaG000100 MtrunA17_Chr5g0424801 28.902 346 215 11 40 373 9 335 6.41e-29 119
MsaG000100 MtrunA17_Chr5g0425661 31.863 204 133 3 39 239 33 233 3.64e-28 114
MsaG000100 MtrunA17_Chr5g0425261 28.902 346 215 11 40 373 9 335 5.25e-28 117
MsaG000100 MtrunA17_Chr5g0424791 28.082 146 97 2 94 239 14 151 1.39e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000100 AT1G19850.1 59.055 889 291 22 19 859 32 895 0.0 954
MsaG000100 AT1G19850.2 59.055 889 291 22 19 859 21 884 0.0 953
MsaG000100 AT1G30330.1 66.038 371 119 4 31 395 11 380 1.36e-159 490
MsaG000100 AT1G30330.1 51.667 120 58 0 734 853 774 893 4.48e-31 131
MsaG000100 AT1G30330.3 65.768 371 120 4 31 395 13 382 1.72e-158 487
MsaG000100 AT1G30330.3 51.667 120 58 0 734 853 776 895 4.65e-31 131
MsaG000100 AT1G30330.2 65.768 371 120 4 31 395 13 382 1.72e-158 487
MsaG000100 AT1G30330.2 51.667 120 58 0 734 853 776 895 4.65e-31 131
MsaG000100 AT1G19220.1 66.488 373 114 6 35 400 15 383 9.52e-158 490
MsaG000100 AT1G19220.1 44.578 166 84 4 684 846 890 1050 4.08e-32 135
MsaG000100 AT5G37020.2 64.960 371 122 5 31 395 11 379 5.26e-157 478
MsaG000100 AT5G37020.2 53.623 69 32 0 754 822 705 773 8.15e-18 89.0
MsaG000100 AT5G37020.1 64.960 371 122 5 31 395 11 379 2.93e-156 478
MsaG000100 AT5G37020.1 51.613 93 45 0 754 846 705 797 2.49e-24 110
MsaG000100 AT5G20730.2 68.222 343 103 4 35 373 16 356 2.76e-150 472
MsaG000100 AT5G20730.2 59.406 101 41 0 746 846 1029 1129 1.72e-31 133
MsaG000100 AT5G20730.3 68.023 344 103 5 35 373 16 357 1.78e-148 467
MsaG000100 AT5G20730.3 59.406 101 41 0 746 846 1030 1130 1.80e-31 133
MsaG000100 AT5G20730.1 68.023 344 103 5 35 373 16 357 2.16e-148 467
MsaG000100 AT5G20730.1 59.406 101 41 0 746 846 1030 1130 1.78e-31 133
MsaG000100 AT5G37020.3 65.614 285 94 3 114 395 1 284 7.86e-118 374
MsaG000100 AT5G37020.3 51.613 93 45 0 754 846 610 702 1.51e-24 110
MsaG000100 AT5G62000.2 54.386 342 144 5 39 373 57 393 6.63e-116 373
MsaG000100 AT5G62000.2 37.931 145 79 4 703 842 683 821 2.38e-18 90.9
MsaG000100 AT5G62000.5 54.386 342 144 5 39 373 57 393 6.63e-116 373
MsaG000100 AT5G62000.5 37.931 145 79 4 703 842 683 821 2.38e-18 90.9
MsaG000100 AT5G62000.3 54.386 342 144 5 39 373 57 393 6.63e-116 373
MsaG000100 AT5G62000.3 37.931 145 79 4 703 842 683 821 2.38e-18 90.9
MsaG000100 AT5G62000.1 54.386 342 144 5 39 373 57 393 6.63e-116 373
MsaG000100 AT5G62000.1 37.931 145 79 4 703 842 683 821 2.38e-18 90.9
MsaG000100 AT5G62000.4 54.386 342 144 5 39 373 57 393 1.25e-115 372
MsaG000100 AT5G62000.4 35.838 173 88 6 703 858 683 849 1.11e-18 92.0
MsaG000100 AT1G59750.4 51.754 342 152 5 39 373 18 353 1.87e-108 348
MsaG000100 AT1G59750.4 48.039 102 49 3 753 852 536 635 6.50e-19 92.4
MsaG000100 AT1G59750.2 51.754 342 152 5 39 373 18 353 2.41e-108 348
MsaG000100 AT1G59750.2 48.039 102 49 3 753 852 538 637 6.76e-19 92.4
MsaG000100 AT1G59750.1 51.754 342 152 5 39 373 18 353 2.69e-108 348
MsaG000100 AT1G59750.1 48.039 102 49 3 753 852 541 640 6.46e-19 92.4
MsaG000100 AT1G59750.3 51.754 342 152 5 39 373 18 353 2.69e-108 348
MsaG000100 AT1G59750.3 48.039 102 49 3 753 852 541 640 6.46e-19 92.4
MsaG000100 AT2G46530.1 49.429 350 166 6 40 383 18 362 6.60e-106 339
MsaG000100 AT2G46530.1 40.000 95 54 2 754 847 490 582 1.84e-14 77.8
MsaG000100 AT2G46530.3 49.429 350 166 6 40 383 39 383 1.40e-105 339
MsaG000100 AT2G46530.3 40.000 95 54 2 754 847 511 603 2.41e-14 77.4
MsaG000100 AT3G61830.1 50.147 339 158 6 40 373 22 354 2.31e-104 335
MsaG000100 AT3G61830.1 46.739 92 44 3 754 843 489 577 8.02e-15 79.0
MsaG000100 AT5G60450.2 49.300 357 165 7 35 378 33 386 1.83e-102 335
MsaG000100 AT5G60450.2 38.835 103 61 2 754 855 641 742 1.80e-16 84.7
MsaG000100 AT5G60450.1 49.716 352 161 7 40 378 62 410 8.43e-102 334
MsaG000100 AT5G60450.1 38.835 103 61 2 754 855 665 766 1.91e-16 84.7
MsaG000100 AT4G23980.2 50.581 344 150 7 40 373 9 342 7.87e-100 324
MsaG000100 AT4G23980.2 46.154 91 46 2 754 843 521 609 2.25e-15 80.9
MsaG000100 AT4G23980.1 50.581 344 150 7 40 373 9 342 8.04e-100 324
MsaG000100 AT4G23980.1 46.154 91 46 2 754 843 523 611 2.32e-15 80.9
MsaG000100 AT2G33860.1 47.714 350 159 5 43 378 54 393 3.09e-98 319
MsaG000100 AT3G61830.2 50.485 309 146 5 66 373 1 303 1.36e-94 308
MsaG000100 AT3G61830.2 46.739 92 44 3 754 843 438 526 6.61e-15 79.3
MsaG000100 AT1G34410.1 43.567 342 182 6 35 373 19 352 2.95e-88 293
MsaG000100 AT1G34410.1 40.909 88 49 2 754 840 511 596 4.35e-12 70.1
MsaG000100 AT1G35540.1 42.398 342 186 7 35 373 19 352 3.64e-85 284
MsaG000100 AT1G35540.1 40.000 90 51 2 754 842 510 597 1.97e-12 71.2
MsaG000100 AT1G35240.1 42.398 342 186 6 35 373 19 352 4.03e-85 284
MsaG000100 AT1G35240.1 42.045 88 48 2 754 840 497 582 2.60e-12 70.9
MsaG000100 AT1G35520.1 43.413 334 178 7 43 373 27 352 2.05e-83 279
MsaG000100 AT1G35520.1 40.741 81 45 2 754 833 511 589 2.40e-11 67.8
MsaG000100 AT1G34310.1 42.105 342 187 7 35 373 19 352 1.98e-81 274
MsaG000100 AT1G34310.1 39.506 81 46 2 754 833 511 589 6.18e-11 66.6
MsaG000100 AT1G34390.1 43.567 342 180 8 35 373 19 350 1.09e-80 272
MsaG000100 AT2G46530.2 50.545 275 128 6 113 383 5 275 1.63e-78 264
MsaG000100 AT2G46530.2 40.000 95 54 2 754 847 403 495 1.14e-14 78.2
MsaG000100 AT2G33860.2 54.661 236 106 1 143 378 2 236 6.00e-78 260
MsaG000100 AT1G34170.1 39.583 336 190 7 43 373 25 352 5.59e-75 254
MsaG000100 AT2G28350.2 37.958 382 186 7 38 373 5 381 2.52e-73 254
MsaG000100 AT2G28350.1 37.958 382 186 7 38 373 5 381 4.77e-73 254
MsaG000100 AT4G30080.1 37.817 394 194 10 35 386 11 395 1.05e-72 252
MsaG000100 AT1G34170.2 37.500 336 171 6 43 373 25 326 2.46e-68 235
MsaG000100 AT1G34170.3 37.500 336 171 6 43 373 25 326 1.37e-67 235
MsaG000100 AT1G77850.1 35.215 372 199 8 37 373 13 377 1.11e-56 205
MsaG000100 AT1G77850.2 35.310 371 198 8 38 373 14 377 1.26e-56 206
MsaG000100 AT1G43950.1 41.706 211 113 6 35 242 19 222 3.98e-42 154

Find 171 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACAATGAATGCATGTTAA+AGG 0.173174 1:-1387446 None:intergenic
CTTCTTATTCTGCAGATATA+TGG 0.173372 1:+1386708 MsaT000100.1:intron
GCCGTTGCAATCCGGATATT+TGG 0.177793 1:+1387064 MsaT000100.1:CDS
AAATACAGAAAATCTGTTTA+TGG 0.194457 1:+1386539 MsaT000100.1:CDS
GGAAAGATGAAAAGGCTTTC+TGG 0.205659 1:-1387017 None:intergenic
CATAATTATCCCACACTTCT+TGG 0.212825 1:-1387852 None:intergenic
AGACATTAAATTCAGAATTA+TGG 0.240617 1:+1384540 MsaT000100.1:CDS
TTAGAACCAGGCCACCTTAA+AGG 0.260988 1:-1386760 None:intergenic
AGCAAAATCGGGTTAGTGTT+TGG 0.286566 1:+1386982 MsaT000100.1:CDS
CTTTCAGCTGGCATGAGATT+TGG 0.299456 1:+1386569 MsaT000100.1:CDS
TTGTTGCATGATAGGTAAAA+AGG 0.323540 1:-1387320 None:intergenic
CGCGCAATTGAATGCATGTT+TGG 0.329869 1:+1388428 MsaT000100.1:CDS
TTCTAAGTGGCGAAATATTC+AGG 0.333372 1:+1386777 MsaT000100.1:CDS
TTCTTATTCTGCAGATATAT+GGG 0.354261 1:+1386709 MsaT000100.1:intron
TGAGCAAAAGTCGTCGAAAA+TGG 0.355292 1:-1387995 None:intergenic
CCGTTGCAATCCGGATATTT+GGG 0.360826 1:+1387065 MsaT000100.1:CDS
TGCATGTTAAAGGTGTTATT+AGG 0.363217 1:-1387436 None:intergenic
GCAAAATCGGGTTAGTGTTT+GGG 0.363557 1:+1386983 MsaT000100.1:CDS
TGCTAGTTGGAGATGATCCT+TGG 0.365242 1:+1388525 MsaT000100.1:CDS
GATGGAAGACTAGGGTAGTT+TGG 0.373410 1:-1384861 None:intergenic
GCATAATCTTGTTGCATGAT+AGG 0.375254 1:-1387328 None:intergenic
AATACAGAAAATCTGTTTAT+GGG 0.375476 1:+1386540 MsaT000100.1:CDS
GTTTGTTTGTCGGCTCAAAA+AGG 0.375508 1:+1385919 MsaT000100.1:CDS
GCTGTTTACAATAGAAGAAT+CGG 0.384358 1:-1387743 None:intergenic
TTTGATTATAATATGTTAGT+TGG 0.389191 1:-1387609 None:intergenic
GTGTTTCCGGTATCTGAGTT+TGG 0.395238 1:+1385188 MsaT000100.1:CDS
TTGTAAATAAACAGGCTGAA+TGG 0.397672 1:+1386937 MsaT000100.1:intron
CACACTTCTTGGTTCATTGA+AGG 0.399760 1:-1387841 None:intergenic
GTGCTTGAAAGGTGAACTTT+CGG 0.411398 1:-1387403 None:intergenic
GTTAAAGAGATGAGTGAAGA+AGG 0.411917 1:+1388743 MsaT000100.1:CDS
CATGGCTTCCTTAGAAGAAA+AGG 0.416918 1:+1384299 None:intergenic
TGTAAATAAACAGGCTGAAT+GGG 0.419597 1:+1386938 MsaT000100.1:intron
TTCTGCAGAATAATAACTCT+TGG 0.422237 1:+1388193 MsaT000100.1:CDS
TCAGTGATGTCGATCCTTTA+AGG 0.425306 1:+1386746 MsaT000100.1:CDS
CTGAATGACACAAAAGGTTC+CGG 0.425971 1:+1388464 MsaT000100.1:CDS
ATGTTACCTTTGTGTAGGTT+CGG 0.426676 1:-1388236 None:intergenic
CACCCTCAAGATTGCATGGT+TGG 0.430745 1:+1388039 MsaT000100.1:CDS
GATGTTTGAGACCGAGGAAT+CGG 0.432905 1:+1386595 MsaT000100.1:CDS
GAACAACTAGTTCTTGAGTT+GGG 0.432917 1:-1385434 None:intergenic
GGTGTGGTGATAAGCAAAAT+CGG 0.435666 1:+1386970 MsaT000100.1:CDS
GTCGATCCTTTAAGGTGGCC+TGG 0.437670 1:+1386754 MsaT000100.1:CDS
CTTGTGTCATTGCCTCAAGT+GGG 0.444777 1:+1384581 MsaT000100.1:CDS
TGGCAACTTAAGTTCTAGTC+AGG 0.445947 1:+1388059 MsaT000100.1:CDS
TATTTGCAGGGCATGTCCTT+CGG 0.447302 1:+1386496 MsaT000100.1:intron
CTAAAACGGCCGTTGCAATC+CGG 0.447348 1:+1387056 MsaT000100.1:CDS
CTTTGACTTCAAGCCTAAAA+CGG 0.447527 1:+1387042 MsaT000100.1:CDS
CTCATCTCTTTAACTTCTGA+AGG 0.449266 1:-1388734 None:intergenic
GATCTTGTGTAGGGAATTTG+TGG 0.451328 1:+1388682 MsaT000100.1:intron
ATTGTTGAAAAGTGAGCATC+CGG 0.452172 1:+1387462 MsaT000100.1:CDS
CGCAATTGCGAGTTGGTGTA+AGG 0.454613 1:+1386260 MsaT000100.1:CDS
GGAAAAGCTGTTTCCTCCAT+TGG 0.458270 1:+1385310 MsaT000100.1:CDS
AGATATATGGGTACAATTGT+AGG 0.463723 1:+1386721 MsaT000100.1:intron
TATGGGACTCACCTTTCAGC+TGG 0.465604 1:+1386557 MsaT000100.1:CDS
AAAAGTGTTGGAGGAGTTGT+TGG 0.469435 1:+1384324 MsaT000100.1:CDS
GAATTATGGCATGCTTGTGC+TGG 0.470225 1:+1384554 MsaT000100.1:CDS
GCTAGTTGGAGATGATCCTT+GGG 0.470617 1:+1388526 MsaT000100.1:CDS
TCAAAGAAGGAAAGATGAAA+AGG 0.471141 1:-1387025 None:intergenic
TTTAGTCCAAACTCAGATAC+CGG 0.471165 1:-1385194 None:intergenic
TTTAGGCTTGAAGTCAAAGA+AGG 0.471500 1:-1387038 None:intergenic
ATTAAAGTACCCGAAAATGA+AGG 0.474465 1:+1387205 MsaT000100.1:CDS
TTGTTGCTGCTTTCAAAAGA+TGG 0.474837 1:-1387562 None:intergenic
CATTGACACAAATTTCACTT+TGG 0.474844 1:-1387918 None:intergenic
AAGAAACGTACCCAAATATC+CGG 0.476983 1:-1387075 None:intergenic
GAGAGTGATGTTCTGCTAGT+TGG 0.482377 1:+1388512 MsaT000100.1:CDS
TAATAGTGTCAGTACCACTA+TGG 0.485813 1:+1387951 MsaT000100.1:CDS
TCTTGTGTCATTGCCTCAAG+TGG 0.485817 1:+1384580 MsaT000100.1:CDS
AAGAGACACCTTCTCACAAC+GGG 0.488034 1:+1385891 MsaT000100.1:CDS
ACGGGTTGGAGTTTGTTTGT+CGG 0.492443 1:+1385909 MsaT000100.1:CDS
TCTAACTGAGGCTGCTGCAT+TGG 0.492863 1:-1387679 None:intergenic
AGCTTACAGAATTCAGTGGT+TGG 0.493036 1:-1385056 None:intergenic
GAAGAAAAGGCTAAAAGTGT+TGG 0.493255 1:+1384312 MsaT000100.1:CDS
ACCTTTGTGTAGGTTCGGAT+TGG 0.493613 1:-1388231 None:intergenic
CCATTGTTGTTGATCACTTG+TGG 0.494094 1:-1387295 None:intergenic
AAAATGGTGTTTGAAGTAGA+AGG 0.495433 1:-1387979 None:intergenic
GGGAAGTCAAACATATGATC+AGG 0.498477 1:-1387541 None:intergenic
GAAAAGGCTAAAAGTGTTGG+AGG 0.498865 1:+1384315 MsaT000100.1:CDS
ATGTTTGAGACCGAGGAATC+GGG 0.501076 1:+1386596 MsaT000100.1:CDS
AGAGTTGTCTCGCAGAGAAA+AGG 0.502279 1:-1388127 None:intergenic
CAGGCTGAATGGGATGAGCC+AGG 0.504340 1:+1386948 MsaT000100.1:intron
ATCTTGTGTAGGGAATTTGT+GGG 0.505590 1:+1388683 MsaT000100.1:intron
AACAACTAGTTCTTGAGTTG+GGG 0.512403 1:-1385433 None:intergenic
CTGTTTACAATAGAAGAATC+GGG 0.516285 1:-1387742 None:intergenic
CTCAGATAGACGTGGACGAA+TGG 0.517978 1:+1387773 MsaT000100.1:CDS
TACGTATACTAGTTTCCATC+CGG 0.523910 1:-1388483 None:intergenic
TGTGTCATTGCCTCAAGTGG+GGG 0.524294 1:+1384583 MsaT000100.1:CDS
TTAAAGAGATGAGTGAAGAA+GGG 0.524881 1:+1388744 MsaT000100.1:CDS
TGCCAACCATGCAATCTTGA+GGG 0.526265 1:-1388041 None:intergenic
CCGAGGAATCGGGTAAGCGC+AGG 0.529760 1:+1386606 MsaT000100.1:CDS
GAGATTTGCATGATAATACA+TGG 0.533645 1:+1385457 MsaT000100.1:CDS
GAATGGGATGAGCCAGGGTG+TGG 0.543031 1:+1386954 MsaT000100.1:CDS
TTCGGTTCCACGCAGAGCTG+CGG 0.543545 1:+1385289 MsaT000100.1:CDS
CCTGCGCTTACCCGATTCCT+CGG 0.545092 1:-1386606 None:intergenic
TTGTGTCATTGCCTCAAGTG+GGG 0.545165 1:+1384582 MsaT000100.1:CDS
GTGTGGTGATAAGCAAAATC+GGG 0.545815 1:+1386971 MsaT000100.1:CDS
CGAACAACTAGTTCTTGAGT+TGG 0.551901 1:-1385435 None:intergenic
TCAGCACCCTCAAGATTGCA+TGG 0.555417 1:+1388035 MsaT000100.1:CDS
GTATGCTCTCTGTGCTTGAA+AGG 0.557270 1:-1387414 None:intergenic
TGGTATGATGTTTGAGACCG+AGG 0.557923 1:+1386589 MsaT000100.1:CDS
TGGAGCATGTCAATCATTTG+AGG 0.562075 1:-1387718 None:intergenic
TTCAATGAACCAAGAAGTGT+GGG 0.563061 1:+1387843 MsaT000100.1:CDS
TAGCAGAAGTAATCTGAGAC+TGG 0.563668 1:-1388086 None:intergenic
TCAGCAGAAAGTACTGATGA+TGG 0.564399 1:-1386310 None:intergenic
AACAACTGAGATGGAAGACT+AGG 0.565825 1:-1384870 None:intergenic
CAAATCTCATGCCAGCTGAA+AGG 0.565921 1:-1386568 None:intergenic
TGTGGACTTCATATCTTGCA+AGG 0.568033 1:-1387365 None:intergenic
CTGAACAGCGGAGCATTGCA+AGG 0.572206 1:+1388776 MsaT000100.1:CDS
TGGTTCATTGAAGGTATCGA+GGG 0.572733 1:-1387832 None:intergenic
AACTCCAACCCGTTGTGAGA+AGG 0.573060 1:-1385899 None:intergenic
AAATAATGTTACCTTTGTGT+AGG 0.573136 1:-1388241 None:intergenic
ACCTGTTCACTATGTCCTTG+AGG 0.574142 1:-1384622 None:intergenic
GTTGTGAGAAGGTGTCTCTT+CGG 0.575157 1:-1385888 None:intergenic
AATTGAATGCATGTTTGGAC+TGG 0.576611 1:+1388433 MsaT000100.1:CDS
GGATTGTAGAAAATCGTGAA+TGG 0.579311 1:-1386388 None:intergenic
CTATGATGCAGAAAATGAGT+GGG 0.579800 1:+1387159 MsaT000100.1:intron
TTGCCAACCATGCAATCTTG+AGG 0.582580 1:-1388042 None:intergenic
CTTCAATGAACCAAGAAGTG+TGG 0.584734 1:+1387842 MsaT000100.1:CDS
TAAGGTGGCCTGGTTCTAAG+TGG 0.586460 1:+1386764 MsaT000100.1:CDS
ATATTTCGCCACTTAGAACC+AGG 0.586883 1:-1386772 None:intergenic
GAACTAGTATGAGCGAGTAA+TGG 0.586998 1:-1387652 None:intergenic
GACACCTTCTCACAACGGGT+TGG 0.588416 1:+1385895 MsaT000100.1:CDS
CTATATAGCCTTACCAATGG+AGG 0.589175 1:-1385323 None:intergenic
GGGTAAGAAACTTACTCCCA+AGG 0.591071 1:-1388542 None:intergenic
ACTTGACACAACAACTGAGA+TGG 0.591159 1:-1384879 None:intergenic
GTGTCATTGCCTCAAGTGGG+GGG 0.591608 1:+1384584 MsaT000100.1:CDS
ATCGAGGGATTCACTACTTG+AGG 0.592078 1:-1387817 None:intergenic
TCGATTAGCAGCTGCATGAG+CGG 0.593060 1:-1386362 None:intergenic
CCCAAATATCCGGATTGCAA+CGG 0.593442 1:-1387065 None:intergenic
TCTTGTCTATTACTTCCTCA+AGG 0.594134 1:+1384607 MsaT000100.1:CDS
AACTTAAGTTCTAGTCAGGA+TGG 0.595500 1:+1388063 MsaT000100.1:CDS
TTACAATAGAAGAATCGGGT+TGG 0.596283 1:-1387738 None:intergenic
TTGGTTCATTGAAGGTATCG+AGG 0.596449 1:-1387833 None:intergenic
AGTGTTGGAGGAGTTGTTGG+TGG 0.597797 1:+1384327 MsaT000100.1:CDS
ATATAGCTTACAGAATTCAG+TGG 0.600450 1:-1385060 None:intergenic
GAATGCATGTTTGGACTGGA+TGG 0.602337 1:+1388437 MsaT000100.1:CDS
TAATTCAAAATTAGAATCTG+AGG 0.603151 1:+1387513 MsaT000100.1:CDS
AGGCTGAATGGGATGAGCCA+GGG 0.604797 1:+1386949 MsaT000100.1:intron
TGTTGCTGCTTTCAAAAGAT+GGG 0.606362 1:-1387561 None:intergenic
GAGAAGTCGCAATTGCGAGT+TGG 0.609632 1:+1386253 MsaT000100.1:CDS
CATTCGTCCACGTCTATCTG+AGG 0.610226 1:-1387772 None:intergenic
ACAACTAGTTCTTGAGTTGG+GGG 0.617960 1:-1385432 None:intergenic
GGTCTGCTGAATGACACAAA+AGG 0.627098 1:+1388458 MsaT000100.1:CDS
ATGACACAAAAGGTTCCGGA+TGG 0.629064 1:+1388468 MsaT000100.1:CDS
TACTTTCATCAAAATCAACA+TGG 0.633033 1:-1388167 None:intergenic
AAAGAAATGCAAGAACATTG+TGG 0.633626 1:+1384381 MsaT000100.1:CDS
CGGATTCTCTATAACCTGTG+TGG 0.635978 1:-1387383 None:intergenic
ACAACTGAGATGGAAGACTA+GGG 0.639765 1:-1384869 None:intergenic
CCACAAGTGATCAACAACAA+TGG 0.640294 1:+1387295 MsaT000100.1:CDS
ACTTGAGGCAATGACACAAG+AGG 0.641095 1:-1384578 None:intergenic
GTGGACTTCATATCTTGCAA+GGG 0.645449 1:-1387364 None:intergenic
GCGAGTGATACTAGCACACA+TGG 0.654643 1:+1385260 MsaT000100.1:CDS
GACATTGATGATTCTAACTG+AGG 0.657820 1:-1387691 None:intergenic
TCTCTGCGAGACAACTCTGG+TGG 0.660933 1:+1388132 MsaT000100.1:CDS
AGTAGAAGGATCAACCATAG+TGG 0.662515 1:-1387965 None:intergenic
ACCCGAAAATGAAGGAACAG+TGG 0.665241 1:+1387213 MsaT000100.1:CDS
CTTGAGGCAATGACACAAGA+GGG 0.667127 1:-1384577 None:intergenic
CAAACATGCATTCAATTGCG+CGG 0.668814 1:-1388427 None:intergenic
GGGATGAAGCTTCTGAACAG+CGG 0.672458 1:+1388764 MsaT000100.1:CDS
CATGATTCCTCAGATAGACG+TGG 0.673580 1:+1387765 MsaT000100.1:CDS
TCTTGTGTAGGGAATTTGTG+GGG 0.680812 1:+1388684 MsaT000100.1:intron
TCTATGATGCAGAAAATGAG+TGG 0.683454 1:+1387158 MsaT000100.1:intron
GCAAGATATGAAGTCCACAC+AGG 0.685567 1:+1387369 MsaT000100.1:CDS
AGTGGAATAACAAAATCCGA+AGG 0.692028 1:-1386512 None:intergenic
GTGATGTCGATCCTTTAAGG+TGG 0.692501 1:+1386749 MsaT000100.1:CDS
AGGGTAGTTTGGAATCTGTG+AGG 0.694049 1:-1384850 None:intergenic
TCTGCTGATAGCATGCACAT+TGG 0.695779 1:+1386325 MsaT000100.1:CDS
TATGATGCAGAAAATGAGTG+GGG 0.696824 1:+1387160 MsaT000100.1:intron
GTGATAATATTCGTATACAG+CGG 0.703562 1:-1388712 None:intergenic
GAAGAGACACCTTCTCACAA+CGG 0.710470 1:+1385890 MsaT000100.1:CDS
ACCAATCCGAACCTACACAA+AGG 0.719172 1:+1388230 MsaT000100.1:CDS
AGTGATACTAGCACACATGG+TGG 0.741127 1:+1385263 MsaT000100.1:CDS
ACATTTCGACATATCTACCG+AGG 0.754798 1:+1385480 MsaT000100.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 1384300 1388811 1384300 ID=MsaG000100
Chr1 mRNA 1384300 1388811 1384300 ID=MsaT000100.1;Parent=MsaG000100
Chr1 exon 1384300 1384402 1384300 ID=MsaT000100.1.exon1;Parent=MsaT000100.1
Chr1 CDS 1384300 1384402 1384300 ID=cds.MsaT000100.1;Parent=MsaT000100.1
Chr1 exon 1384531 1384634 1384531 ID=MsaT000100.1.exon2;Parent=MsaT000100.1
Chr1 CDS 1384531 1384634 1384531 ID=cds.MsaT000100.1;Parent=MsaT000100.1
Chr1 exon 1384822 1384920 1384822 ID=MsaT000100.1.exon3;Parent=MsaT000100.1
Chr1 CDS 1384822 1384920 1384822 ID=cds.MsaT000100.1;Parent=MsaT000100.1
Chr1 exon 1385015 1385071 1385015 ID=MsaT000100.1.exon4;Parent=MsaT000100.1
Chr1 CDS 1385015 1385071 1385015 ID=cds.MsaT000100.1;Parent=MsaT000100.1
Chr1 exon 1385179 1385331 1385179 ID=MsaT000100.1.exon5;Parent=MsaT000100.1
Chr1 CDS 1385179 1385331 1385179 ID=cds.MsaT000100.1;Parent=MsaT000100.1
Chr1 exon 1385417 1385501 1385417 ID=MsaT000100.1.exon6;Parent=MsaT000100.1
Chr1 CDS 1385417 1385501 1385417 ID=cds.MsaT000100.1;Parent=MsaT000100.1
Chr1 exon 1385883 1385973 1385883 ID=MsaT000100.1.exon7;Parent=MsaT000100.1
Chr1 CDS 1385883 1385973 1385883 ID=cds.MsaT000100.1;Parent=MsaT000100.1
Chr1 exon 1386249 1386413 1386249 ID=MsaT000100.1.exon8;Parent=MsaT000100.1
Chr1 CDS 1386249 1386413 1386249 ID=cds.MsaT000100.1;Parent=MsaT000100.1
Chr1 exon 1386505 1386627 1386505 ID=MsaT000100.1.exon9;Parent=MsaT000100.1
Chr1 CDS 1386505 1386627 1386505 ID=cds.MsaT000100.1;Parent=MsaT000100.1
Chr1 exon 1386723 1386798 1386723 ID=MsaT000100.1.exon10;Parent=MsaT000100.1
Chr1 CDS 1386723 1386798 1386723 ID=cds.MsaT000100.1;Parent=MsaT000100.1
Chr1 exon 1386951 1387086 1386951 ID=MsaT000100.1.exon11;Parent=MsaT000100.1
Chr1 CDS 1386951 1387086 1386951 ID=cds.MsaT000100.1;Parent=MsaT000100.1
Chr1 exon 1387170 1388251 1387170 ID=MsaT000100.1.exon12;Parent=MsaT000100.1
Chr1 CDS 1387170 1388251 1387170 ID=cds.MsaT000100.1;Parent=MsaT000100.1
Chr1 exon 1388359 1388549 1388359 ID=MsaT000100.1.exon13;Parent=MsaT000100.1
Chr1 CDS 1388359 1388549 1388359 ID=cds.MsaT000100.1;Parent=MsaT000100.1
Chr1 exon 1388694 1388811 1388694 ID=MsaT000100.1.exon14;Parent=MsaT000100.1
Chr1 CDS 1388694 1388811 1388694 ID=cds.MsaT000100.1;Parent=MsaT000100.1
Gene Sequence

>MsaT000100.1

ATGGCTTCCTTAGAAGAAAAGGCTAAAAGTGTTGGAGGAGTTGTTGGTGGTCAAACTCTTGTTGCTGAAATGAAGTTGCTCAAAGAAATGCAAGAACATTGTGGGGTTAGAAAGACATTAAATTCAGAATTATGGCATGCTTGTGCTGGCCCTCTTGTGTCATTGCCTCAAGTGGGGGGTCTTGTCTATTACTTCCTCAAGGACATAGTTGCAGCTTCTACTAGAAGAATAGCTACCTCACAGATTCCAAACTACCCTAGTCTTCCATCTCAGTTGTTGTGTCAAGTACAAAATGTCACACTACATGCTGACAAGGAGACAGATGAAATCTATGCTCAAATGACTCTCCAACCACTGAATTCTGAAAAAGAAGTGTTTCCGGTATCTGAGTTTGGACTAAACAAAAGTAAACATCCAACTGAGTTTTTCTGCAAAACTTTGACTGCGAGTGATACTAGCACACATGGTGGCTTTTCGGTTCCACGCAGAGCTGCGGAAAAGCTGTTTCCTCCATTGGATTACACAATTCAACCCCCAACTCAAGAACTAGTTGTTCGAGATTTGCATGATAATACATGGACATTTCGACATATCTACCGAGGTCAGCCGAAGAGACACCTTCTCACAACGGGTTGGAGTTTGTTTGTCGGCTCAAAAAGGCTTAAAGCAGGCGATTCTGTTCTTTTTATCAGGGATGAGAAGTCGCAATTGCGAGTTGGTGTAAGGCGTGCTAATCGTCAACAGACAACACTGCCATCATCAGTACTTTCTGCTGATAGCATGCACATTGGTGTGCTTGCTGCTGCCGCTCATGCAGCTGCTAATCGAAGTCCATTCACGATTTTCTACAATCCAAGGGCATGTCCTTCGGATTTTGTTATTCCACTGGCCAAATACAGAAAATCTGTTTATGGGACTCACCTTTCAGCTGGCATGAGATTTGGTATGATGTTTGAGACCGAGGAATCGGGTAAGCGCAGATATATGGGTACAATTGTAGGTATCAGTGATGTCGATCCTTTAAGGTGGCCTGGTTCTAAGTGGCGAAATATTCAGGCTGAATGGGATGAGCCAGGGTGTGGTGATAAGCAAAATCGGGTTAGTGTTTGGGAGATTGAGACTCCAGAAAGCCTTTTCATCTTTCCTTCTTTGACTTCAAGCCTAAAACGGCCGTTGCAATCCGGATATTTGGAAAATGAGTGGGGAACTTTGATACGAAGACCCTTTATTAAAGTACCCGAAAATGAAGGAACAGTGGAGCTCTCAAACTCAATGCCAAATCTCTACCAAGAAAACATGATGAAGATGTTTTATAAACCACAAGTGATCAACAACAATGGAGCCTTTTTACCTATCATGCAACAAGATTATGCTCCTAAAAGAGTTCCCTTGCAAGATATGAAGTCCACACAGGTTATAGAGAATCCGAAAGTTCACCTTTCAAGCACAGAGAGCATACCTAATAACACCTTTAACATGCATTCATTGTTGAAAAGTGAGCATCCGGAAAAGTTGCTGCCTATCGCCAAAACCAATAATTCAAAATTAGAATCTGAGGTGTTACCTGATCATATGTTTGACTTCCCATCTTTTGAAAGCAGCAACAACATTGAAAAAGTGAATACGGCAAACCAACTAACATATTATAATCAAAACCAAAACCAAACTCAAAATCCATTACTCGCTCATACTAGTTCATCGCCAATGCAGCAGCCTCAGTTAGAATCATCAATGTCACATCCTCAAATGATTGACATGCTCCAACCCGATTCTTCTATTGTAAACAGCATGATTCCTCAGATAGACGTGGACGAATGGATGATGTATTCTTCTTGTCAGCCTCAAGTAGTGAATCCCTCGATACCTTCAATGAACCAAGAAGTGTGGGATAATTATGTGAAGAATTTTAACTTGAGGGATCTTTCAGCTGAAAGCAATAACCAAAGTGAAATTTGTGTCAATGTTGATGCTATTAATAGTGTCAGTACCACTATGGTTGATCCTTCTACTTCAAACACCATTTTCGACGACTTTTGCTCAATGAAAGATAAAGATTTTCAGCACCCTCAAGATTGCATGGTTGGCAACTTAAGTTCTAGTCAGGATGGCCAGTCTCAGATTACTTCTGCTAGTCTAGCTGAGTCGCACGCCTTTTCTCTGCGAGACAACTCTGGTGGTACTTCTTCAAGCCATGTTGATTTTGATGAAAGTAGTTTTCTGCAGAATAATAACTCTTGGAAGCAAGTAGCTGCACCAATCCGAACCTACACAAAGGTGCAAAAAGCAGGTTCTGTAGGAAGATCAATTGATGTGACTACTTTCAAGAACTATGAAGAGTTGATCCGCGCAATTGAATGCATGTTTGGACTGGATGGTCTGCTGAATGACACAAAAGGTTCCGGATGGAAACTAGTATACGTAGATTATGAGAGTGATGTTCTGCTAGTTGGAGATGATCCTTGGGAGGAATTTGTGGGGTGTGTCCGCTGTATACGAATATTATCACCTTCAGAAGTTAAAGAGATGAGTGAAGAAGGGATGAAGCTTCTGAACAGCGGAGCATTGCAAGGAATCAATGTGTGA
Protein sequence

>MsaT000100.1

MASLEEKAKSVGGVVGGQTLVAEMKLLKEMQEHCGVRKTLNSELWHACAGPLVSLPQVGGLVYYFLKDIVAASTRRIATSQIPNYPSLPSQLLCQVQNVTLHADKETDEIYAQMTLQPLNSEKEVFPVSEFGLNKSKHPTEFFCKTLTASDTSTHGGFSVPRRAAEKLFPPLDYTIQPPTQELVVRDLHDNTWTFRHIYRGQPKRHLLTTGWSLFVGSKRLKAGDSVLFIRDEKSQLRVGVRRANRQQTTLPSSVLSADSMHIGVLAAAAHAAANRSPFTIFYNPRACPSDFVIPLAKYRKSVYGTHLSAGMRFGMMFETEESGKRRYMGTIVGISDVDPLRWPGSKWRNIQAEWDEPGCGDKQNRVSVWEIETPESLFIFPSLTSSLKRPLQSGYLENEWGTLIRRPFIKVPENEGTVELSNSMPNLYQENMMKMFYKPQVINNNGAFLPIMQQDYAPKRVPLQDMKSTQVIENPKVHLSSTESIPNNTFNMHSLLKSEHPEKLLPIAKTNNSKLESEVLPDHMFDFPSFESSNNIEKVNTANQLTYYNQNQNQTQNPLLAHTSSSPMQQPQLESSMSHPQMIDMLQPDSSIVNSMIPQIDVDEWMMYSSCQPQVVNPSIPSMNQEVWDNYVKNFNLRDLSAESNNQSEICVNVDAINSVSTTMVDPSTSNTIFDDFCSMKDKDFQHPQDCMVGNLSSSQDGQSQITSASLAESHAFSLRDNSGGTSSSHVDFDESSFLQNNNSWKQVAAPIRTYTKVQKAGSVGRSIDVTTFKNYEELIRAIECMFGLDGLLNDTKGSGWKLVYVDYESDVLLVGDDPWEEFVGCVRCIRILSPSEVKEMSEEGMKLLNSGALQGINV*