AlfalfaGEDB | MsaG000101

M. sativa ssp. caerulea / MsaG000101

KEGG: K14486
GO: GO:0000003 GO:0000578 GO:0001067 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0007389 GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009880 GO:0009888 GO:0009889 GO:0009908 GO:0009942 GO:0010033 GO:0010051 GO:0010154 GO:0010305 GO:0010468 GO:0010556 GO:0016020 GO:0019219 GO:0019222 GO:0022414 GO:0022622 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0042221 GO:0042802 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044212 GO:0044424 GO:0044464 GO:0048316 GO:0048364 GO:0048367 GO:0048507 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0090567 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02362.24

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000101	KEH40213.1	58.519	270	64	7	35	263	846	1108	1.87e-78	270
MsaG000101	KEH40213.1	63.359	131	39	4	83	206	330	458	2.42e-40	160
MsaG000101	KEH40213.1	91.379	116	10	0	148	263	569	684	3.16e-60	218
MsaG000101	KEH40213.1	92.857	56	4	0	19	74	204	259	2.82e-22	108

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000101	sp\|P93024\|ARFE_ARATH	46.743	261	65	8	19	207	216	474	1.44e-53	190

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000101	A0A072VFI0	58.519	270	64	7	35	263	846	1108	8.94e-79	270
MsaG000101	A0A072VFI0	63.359	131	39	4	83	206	330	458	1.15e-40	160
MsaG000101	A0A072VFI0	91.379	116	10	0	148	263	569	684	1.51e-60	218
MsaG000101	A0A072VFI0	92.857	56	4	0	19	74	204	259	1.35e-22	108

TFs/TRs:

Gene ID	Type	Classification
MsaG000101	TF	B3-ARF

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000101	MtrunA17_Chr1g0156301	90.654	107	10	0	157	263	1	107	1.55e-65	206
MsaG000101	MtrunA17_Chr1g0156321	91.379	116	10	0	148	263	569	684	4.10e-65	218
MsaG000101	MtrunA17_Chr1g0156321	63.359	131	39	4	83	206	330	458	1.94e-44	160
MsaG000101	MtrunA17_Chr1g0156321	92.857	56	4	0	19	74	204	259	2.78e-26	108
MsaG000101	MtrunA17_Chr3g0109391	71.429	56	16	0	19	74	188	243	2.68e-18	85.1
MsaG000101	MtrunA17_Chr3g0109391	51.429	70	33	1	83	151	314	383	2.53e-15	76.3
MsaG000101	MtrunA17_Chr2g0285461	59.740	77	25	2	2	74	166	240	8.31e-18	83.6
MsaG000101	MtrunA17_Chr2g0285461	50.000	70	34	1	83	151	311	380	5.89e-15	75.1
MsaG000101	MtrunA17_Chr4g0070181	74.510	51	13	0	19	69	184	234	1.23e-17	83.2
MsaG000101	MtrunA17_Chr4g0070181	59.574	47	19	0	83	129	309	355	3.87e-14	72.8
MsaG000101	MtrunA17_Chr5g0433301	69.643	56	17	0	19	74	186	241	1.70e-17	82.4
MsaG000101	MtrunA17_Chr5g0433301	52.857	70	32	1	83	151	312	381	1.06e-16	80.1
MsaG000101	MtrunA17_Chr8g0389081	77.551	49	11	0	19	67	194	242	1.79e-17	82.4
MsaG000101	MtrunA17_Chr8g0389081	57.447	47	20	0	83	129	320	366	5.23e-14	72.4
MsaG000101	MtrunA17_Chr2g0276781	51.190	84	35	2	2	81	164	245	3.12e-17	81.6
MsaG000101	MtrunA17_Chr8g0385791	51.190	84	35	2	2	81	201	282	4.15e-17	81.3
MsaG000101	MtrunA17_Chr8g0346711	32.941	170	55	5	19	129	189	358	8.43e-17	80.5
MsaG000101	MtrunA17_Chr8g0376771	67.857	56	18	0	19	74	184	239	9.51e-17	80.5
MsaG000101	MtrunA17_Chr8g0376771	51.429	70	33	1	83	151	310	379	1.25e-15	77.0
MsaG000101	MtrunA17_Chr2g0301251	70.588	51	15	0	19	69	184	234	1.56e-16	79.7
MsaG000101	MtrunA17_Chr2g0301251	61.702	47	18	0	83	129	309	355	3.07e-14	72.8
MsaG000101	MtrunA17_Chr2g0326281	55.357	56	25	0	19	74	239	294	3.68e-14	72.8
MsaG000101	MtrunA17_Chr4g0029671	55.357	56	25	0	19	74	224	279	8.76e-14	71.6
MsaG000101	MtrunA17_Chr7g0239061	57.143	56	24	0	19	74	174	229	2.29e-13	70.1
MsaG000101	MtrunA17_Chr4g0009751	60.714	56	20	1	10	65	172	225	7.68e-13	68.6
MsaG000101	MtrunA17_Chr1g0156311	80.645	31	6	0	108	138	59	89	7.16e-12	60.5
MsaG000101	MtrunA17_Chr7g0264171	59.574	47	19	0	19	65	189	235	2.07e-11	64.3
MsaG000101	MtrunA17_Chr1g0199681	51.667	60	27	1	5	64	239	296	2.97e-11	63.9
MsaG000101	MtrunA17_Chr4g0046371	61.364	44	17	0	19	62	206	249	4.10e-11	63.5
MsaG000101	MtrunA17_Chr2g0282961	61.364	44	17	0	19	62	216	259	4.12e-11	63.2
MsaG000101	MtrunA17_Chr2g0282961	38.462	78	48	0	83	160	341	418	6.22e-11	62.8
MsaG000101	MtrunA17_Chr2g0326761	51.667	60	27	1	5	64	154	211	6.72e-11	62.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000101	AT1G19850.1	46.743	261	65	8	19	207	216	474	1.47e-54	190
MsaG000101	AT1G19850.2	46.743	261	65	8	19	207	205	463	1.91e-54	189
MsaG000101	AT2G46530.1	35.602	191	59	6	10	139	175	362	1.26e-24	103
MsaG000101	AT2G46530.2	35.602	191	59	6	10	139	88	275	1.35e-24	103
MsaG000101	AT2G46530.3	35.602	191	59	6	10	139	196	383	1.47e-24	103
MsaG000101	AT3G61830.2	36.313	179	52	5	10	129	128	303	5.43e-24	101
MsaG000101	AT5G62000.2	34.197	193	60	4	2	129	203	393	5.85e-24	102
MsaG000101	AT5G62000.5	34.197	193	60	4	2	129	203	393	5.85e-24	102
MsaG000101	AT5G62000.3	34.197	193	60	4	2	129	203	393	5.85e-24	102
MsaG000101	AT5G62000.1	34.197	193	60	4	2	129	203	393	5.85e-24	102
MsaG000101	AT3G61830.1	36.313	179	52	5	10	129	179	354	6.93e-24	101
MsaG000101	AT5G62000.4	34.197	193	60	4	2	129	203	393	7.76e-24	101
MsaG000101	AT5G60450.2	34.091	176	56	2	19	134	211	386	6.86e-22	95.9
MsaG000101	AT2G33860.2	33.898	177	56	3	19	134	60	236	2.59e-21	93.6
MsaG000101	AT2G33860.1	33.898	177	56	3	19	134	217	393	3.05e-21	94.0
MsaG000101	AT4G23980.2	35.882	170	49	3	19	129	174	342	4.55e-20	90.5
MsaG000101	AT4G23980.1	35.882	170	49	3	19	129	174	342	4.68e-20	90.5
MsaG000101	AT1G35540.1	32.065	184	63	5	5	129	172	352	4.63e-19	87.4
MsaG000101	AT1G34410.1	32.941	170	54	4	19	129	184	352	4.95e-19	87.4
MsaG000101	AT1G35240.1	24.742	388	164	12	19	286	184	563	1.92e-18	85.5
MsaG000101	AT1G35520.1	32.941	170	54	4	19	129	184	352	2.46e-18	85.1
MsaG000101	AT1G19220.1	76.471	51	12	0	19	69	184	234	3.18e-18	85.1
MsaG000101	AT1G19220.1	54.167	72	28	2	83	151	309	378	1.67e-16	80.1
MsaG000101	AT1G30330.3	59.740	77	25	2	2	74	168	242	8.18e-18	84.0
MsaG000101	AT1G30330.3	50.000	70	34	1	83	151	313	382	1.30e-14	74.3
MsaG000101	AT1G30330.2	59.740	77	25	2	2	74	168	242	8.18e-18	84.0
MsaG000101	AT1G30330.2	50.000	70	34	1	83	151	313	382	1.30e-14	74.3
MsaG000101	AT1G30330.1	59.740	77	25	2	2	74	166	240	8.33e-18	84.0
MsaG000101	AT1G30330.1	50.000	70	34	1	83	151	311	380	1.34e-14	74.3
MsaG000101	AT1G59750.2	60.656	61	24	0	19	79	182	242	1.44e-17	83.2
MsaG000101	AT1G59750.1	60.656	61	24	0	19	79	182	242	1.44e-17	83.2
MsaG000101	AT1G59750.3	60.656	61	24	0	19	79	182	242	1.44e-17	83.2
MsaG000101	AT1G59750.4	60.656	61	24	0	19	79	182	242	1.49e-17	83.2
MsaG000101	AT5G37020.2	67.857	56	18	0	19	74	184	239	2.99e-17	82.4
MsaG000101	AT5G37020.2	52.857	70	32	1	83	151	310	379	1.16e-16	80.5
MsaG000101	AT5G37020.1	67.857	56	18	0	19	74	184	239	3.15e-17	82.0
MsaG000101	AT5G37020.1	52.857	70	32	1	83	151	310	379	1.28e-16	80.5
MsaG000101	AT5G37020.3	67.857	56	18	0	19	74	89	144	3.19e-17	82.0
MsaG000101	AT5G37020.3	52.857	70	32	1	83	151	215	284	1.17e-16	80.5
MsaG000101	AT5G20730.2	74.510	51	13	0	19	69	185	235	4.25e-17	82.0
MsaG000101	AT5G20730.2	37.681	138	65	6	83	203	310	443	1.31e-15	77.4
MsaG000101	AT5G20730.1	74.510	51	13	0	19	69	185	235	4.50e-17	82.0
MsaG000101	AT5G20730.1	37.681	138	65	6	83	203	311	444	1.30e-15	77.4
MsaG000101	AT5G20730.3	74.510	51	13	0	19	69	185	235	4.70e-17	81.6
MsaG000101	AT5G20730.3	37.681	138	65	6	83	203	311	444	1.57e-15	77.4
MsaG000101	AT1G34310.1	56.897	58	25	0	19	76	184	241	6.95e-15	75.1
MsaG000101	AT5G60450.1	56.140	57	25	0	19	75	235	291	6.97e-15	75.1
MsaG000101	AT1G34390.1	58.333	48	20	0	19	66	182	229	1.23e-11	65.5

Find 69 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCCGTTACAATCCGGATATT+TGG	0.204347	1:+1390904	MsaT000101.1:CDS
CATATTGATCCCACACTTCT+TGG	0.244067	1:-1391923	None:intergenic
GGAAAGATGAAAAGGCTTTC+CGG	0.284572	1:-1390857	None:intergenic
TGCTTGTTGGAGATGATCCC+TGG	0.287087	1:+1392147	MsaT000101.1:CDS
AGCAAAATAGGGTTAGTGTT+TGG	0.311735	1:+1390822	MsaT000101.1:CDS
GCAAAATAGGGTTAGTGTTT+GGG	0.321539	1:+1390823	MsaT000101.1:CDS
TTCTAAGTGGCGAAATATTC+AGG	0.333372	1:+1390639	MsaT000101.1:CDS
CCGTTACAATCCGGATATTT+GGG	0.343089	1:+1390905	MsaT000101.1:CDS
TGAGCAAAAGTCGTCGAAAA+TGG	0.355292	1:-1392066	None:intergenic
GGTGTGGTGATAAGCAAAAT+AGG	0.358353	1:+1390810	MsaT000101.1:CDS
GTTTGTTTGTCGGATCAAAA+AGG	0.368222	1:+1390150	MsaT000101.1:CDS
TGGTTCATTGAGGGTATAAA+GGG	0.388280	1:-1391903	None:intergenic
TTGGTTCATTGAGGGTATAA+AGG	0.399826	1:-1391904	None:intergenic
CCCGATTCTTCTATTGTAAA+CGG	0.400606	1:+1391813	MsaT000101.1:CDS
GCCGTTTACAATAGAAGAAT+CGG	0.404471	1:-1391814	None:intergenic
CTTAGAACCAGGCCACCTTA+AGG	0.409021	1:-1390623	None:intergenic
TTAGAACCAGGCCACCTTAA+GGG	0.411042	1:-1390622	None:intergenic
GAGAGTGATGTTCTGCTTGT+TGG	0.411875	1:+1392134	MsaT000101.1:CDS
TTGTAAATAAACAGGCTGAA+TGG	0.413200	1:+1390777	MsaT000101.1:intron
TGTAAATAAACAGGCTGAAT+GGG	0.419597	1:+1390778	MsaT000101.1:intron
AACTCTGGAATGGTGATTGC+CGG	0.434268	1:+1389615	MsaT000101.1:CDS
AAAATGGTGTTTGAAGTAGA+AGG	0.438666	1:-1392050	None:intergenic
CACACTTCTTGGTTCATTGA+GGG	0.439145	1:-1391912	None:intergenic
TTTAGACTTGAAGTCAAAGA+AGG	0.444901	1:-1390878	None:intergenic
AAGAAACTTACCCAAATATC+CGG	0.446011	1:-1390915	None:intergenic
CTTTGACTTCAAGTCTAAAA+CGG	0.448239	1:+1390882	MsaT000101.1:CDS
TACATATACTAATTTCCATC+TGG	0.450352	1:-1392105	None:intergenic
CGCAATTGCGAGTTGGTGTA+AGG	0.454613	1:+1390494	MsaT000101.1:CDS
GTCGATCCCTTAAGGTGGCC+TGG	0.457511	1:+1390616	MsaT000101.1:CDS
TCAAAGAAGGAAAGATGAAA+AGG	0.471141	1:-1390865	None:intergenic
AAGAGACACCTTCTCACAAC+GGG	0.474222	1:+1390122	MsaT000101.1:CDS
CATTGACACAAATTTCACTT+TGG	0.474844	1:-1391989	None:intergenic
AGATATATGGGTACAATTGT+TGG	0.476234	1:+1390583	MsaT000101.1:CDS
CCACACTTCTTGGTTCATTG+AGG	0.479579	1:-1391913	None:intergenic
TGATAGTGTGAGTACTACTA+TGG	0.489008	1:+1392022	MsaT000101.1:CDS
CTAAAACGGCCGTTACAATC+CGG	0.494047	1:+1390896	MsaT000101.1:CDS
TGTATATCAACTACTGATGA+TGG	0.494800	1:-1390544	None:intergenic
CAGGCTGAATGGGATGAGCC+AGG	0.504340	1:+1390788	MsaT000101.1:intron
ACGGGTTGGAGTTTGTTTGT+CGG	0.505288	1:+1390140	MsaT000101.1:CDS
GTGTGGTGATAAGCAAAATA+GGG	0.509449	1:+1390811	MsaT000101.1:CDS
CCGTTTACAATAGAAGAATC+GGG	0.516701	1:-1391813	None:intergenic
GCTTGTTGGAGATGATCCCT+GGG	0.527109	1:+1392148	MsaT000101.1:CDS
AGGGTAAGAAACTTACTCCC+AGG	0.529099	1:-1392165	None:intergenic
CATTCGTCAACGTCTAGCTC+AGG	0.532532	1:-1391843	None:intergenic
TTAGTGATGTCGATCCCTTA+AGG	0.537210	1:+1390608	MsaT000101.1:CDS
GAATGGGATGAGCCAGGGTG+TGG	0.543031	1:+1390794	MsaT000101.1:CDS
GAAAAGATATTACGAGGAGA+TGG	0.543102	1:+1389644	MsaT000101.1:CDS
CCCAAATATCCGGATTGTAA+CGG	0.559486	1:-1390905	None:intergenic
CTGAGCTAGACGTTGACGAA+TGG	0.571956	1:+1391844	MsaT000101.1:CDS
AACTCCAACCCGTTGTGAGA+AGG	0.573060	1:-1390130	None:intergenic
CTCAATGAACCAAGAAGTGT+GGG	0.573553	1:+1391914	MsaT000101.1:CDS
GAATTAATGGAACTCTGGAA+TGG	0.574791	1:+1389605	None:intergenic
ATAAAGGGATTCACTACTTG+AGG	0.575174	1:-1391888	None:intergenic
ATATTTCGCCACTTAGAACC+AGG	0.586883	1:-1390634	None:intergenic
GACACCTTCTCACAACGGGT+TGG	0.588416	1:+1390126	MsaT000101.1:CDS
AAATGGTGTTTGAAGTAGAA+GGG	0.593790	1:-1392049	None:intergenic
TGAAGGATAAAGGTTCCAGA+TGG	0.600907	1:+1392090	MsaT000101.1:CDS
TTACAATAGAAGAATCGGGT+TGG	0.604117	1:-1391809	None:intergenic
AGGCTGAATGGGATGAGCCA+GGG	0.604797	1:+1390789	MsaT000101.1:intron
TGTTTGGGAGATCGAGACTC+CGG	0.608961	1:+1390838	MsaT000101.1:CDS
CCTCAATGAACCAAGAAGTG+TGG	0.613123	1:+1391913	MsaT000101.1:CDS
GAGAAGTCGCAATTGCGAGT+TGG	0.615554	1:+1390487	MsaT000101.1:CDS
GGGTAAGAAACTTACTCCCA+GGG	0.623236	1:-1392164	None:intergenic
TGGACCATGTCAATCAGTTG+AGG	0.629987	1:-1391789	None:intergenic
TAAGGTGGCCTGGTTCTAAG+TGG	0.634814	1:+1390626	MsaT000101.1:CDS
ACATCCTCAACTGATTGACA+TGG	0.679013	1:+1391785	MsaT000101.1:CDS
GAAGAGACACCTTCTCACAA+CGG	0.684005	1:+1390121	MsaT000101.1:CDS
GTGATGTCGATCCCTTAAGG+TGG	0.694797	1:+1390611	MsaT000101.1:CDS
AGAAATGAAAAGATATTACG+AGG	0.708127	1:+1389638	MsaT000101.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	1389611	1392175	1389611	ID=MsaG000101
Chr1	mRNA	1389611	1392175	1389611	ID=MsaT000101.1;Parent=MsaG000101
Chr1	exon	1389611	1389665	1389611	ID=MsaT000101.1.exon1;Parent=MsaT000101.1
Chr1	CDS	1389611	1389665	1389611	ID=cds.MsaT000101.1;Parent=MsaT000101.1
Chr1	exon	1390114	1390204	1390114	ID=MsaT000101.1.exon2;Parent=MsaT000101.1
Chr1	CDS	1390114	1390204	1390114	ID=cds.MsaT000101.1;Parent=MsaT000101.1
Chr1	exon	1390483	1390660	1390483	ID=MsaT000101.1.exon3;Parent=MsaT000101.1
Chr1	CDS	1390483	1390660	1390483	ID=cds.MsaT000101.1;Parent=MsaT000101.1
Chr1	exon	1390791	1390926	1390791	ID=MsaT000101.1.exon4;Parent=MsaT000101.1
Chr1	CDS	1390791	1390926	1390791	ID=cds.MsaT000101.1;Parent=MsaT000101.1
Chr1	exon	1391772	1392175	1391772	ID=MsaT000101.1.exon5;Parent=MsaT000101.1
Chr1	CDS	1391772	1392175	1391772	ID=cds.MsaT000101.1;Parent=MsaT000101.1

Gene Sequence

>MsaT000101.1

ATGGAACTCTGGAATGGTGATTGCCGGAGAAATGAAAAGATATTACGAGGAGATGGTCATCTGAAGAGACACCTTCTCACAACGGGTTGGAGTTTGTTTGTCGGATCAAAAAGGCTTAAAGCAGGCGATTCTGTTCTTTTTATCAGGGACGAGAAGTCGCAATTGCGAGTTGGTGTAAGGCGTGCTAATCGTCAACAGACAACACTGCCATCATCAGTAGTTGATATACATTTCCTCTTTTTCTGCAGATATATGGGTACAATTGTTGGTATTAGTGATGTCGATCCCTTAAGGTGGCCTGGTTCTAAGTGGCGAAATATTCAGGCTGAATGGGATGAGCCAGGGTGTGGTGATAAGCAAAATAGGGTTAGTGTTTGGGAGATCGAGACTCCGGAAAGCCTTTTCATCTTTCCTTCTTTGACTTCAAGTCTAAAACGGCCGTTACAATCCGGATATTTGGAATCATCAATGTCACATCCTCAACTGATTGACATGGTCCAACCCGATTCTTCTATTGTAAACGGCACGCTTCCTGAGCTAGACGTTGACGAATGGATGATGTATTCTTCTTGTCAGCCTCAAGTAGTGAATCCCTTTATACCCTCAATGAACCAAGAAGTGTGGGATCAATATGTCAAGAATTTTAACTTGAGAGATCTTTCAGCTGAAAGCAATAACCAAAGTGAAATTTGTGTCAATGTTGATGCTAGTGATAGTGTGAGTACTACTATGGTTGACCCTTCTACTTCAAACACCATTTTCGACGACTTTTGCTCAATGAAGGATAAAGGTTCCAGATGGAAATTAGTATATGTAGATTACGAGAGTGATGTTCTGCTTGTTGGAGATGATCCCTGGGAGTAA

Protein sequence

>MsaT000101.1

MELWNGDCRRNEKILRGDGHLKRHLLTTGWSLFVGSKRLKAGDSVLFIRDEKSQLRVGVRRANRQQTTLPSSVVDIHFLFFCRYMGTIVGISDVDPLRWPGSKWRNIQAEWDEPGCGDKQNRVSVWEIETPESLFIFPSLTSSLKRPLQSGYLESSMSHPQLIDMVQPDSSIVNGTLPELDVDEWMMYSSCQPQVVNPFIPSMNQEVWDQYVKNFNLRDLSAESNNQSEICVNVDASDSVSTTMVDPSTSNTIFDDFCSMKDKGSRWKLVYVDYESDVLLVGDDPWE*