AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG000133

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000133 MtrunA17_Chr1g0155791 97.835 231 3 1 1 229 108 338 2.29e-168 467
MsaG000133 MtrunA17_Chr3g0142171 61.538 221 68 9 11 226 82 290 1.74e-72 222
MsaG000133 MtrunA17_Chr8g0372461 54.132 242 78 7 1 229 110 331 3.19e-68 213
MsaG000133 MtrunA17_Chr5g0404781 54.661 236 90 6 7 229 92 323 5.58e-68 212
MsaG000133 MtrunA17_Chr5g0441921 45.062 162 82 3 15 169 124 285 1.90e-38 136
MsaG000133 MtrunA17_Chr3g0082511 40.331 181 90 5 1 168 119 294 8.34e-36 129
MsaG000133 MtrunA17_Chr4g0035591 41.611 149 62 2 12 160 17 140 2.40e-35 124
MsaG000133 MtrunA17_Chr8g0353111 39.779 181 96 3 15 182 124 304 1.19e-32 120
MsaG000133 MtrunA17_Chr2g0281741 63.158 38 14 0 12 49 147 184 3.43e-13 65.9
MsaG000133 MtrunA17_Chr0c01g0489181 50.000 52 22 1 2 49 50 101 3.47e-12 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000133 AT1G75520.1 52.340 235 87 7 11 229 121 346 1.48e-71 222
MsaG000133 AT3G51060.1 50.211 237 90 5 13 229 142 370 1.49e-68 215
MsaG000133 AT1G19790.2 50.000 244 105 7 1 228 102 344 1.07e-67 212
MsaG000133 AT1G19790.1 50.000 244 105 7 1 228 102 344 1.07e-67 212
MsaG000133 AT5G66350.1 49.333 225 91 6 15 229 120 331 7.01e-64 202
MsaG000133 AT5G66350.3 48.182 220 91 6 15 224 120 326 4.51e-61 194
MsaG000133 AT5G66350.2 49.065 214 86 6 15 218 120 320 6.61e-60 191
MsaG000133 AT2G21400.2 42.791 215 83 6 12 223 7 184 2.87e-48 157
MsaG000133 AT2G21400.4 42.791 215 83 6 12 223 6 183 2.91e-48 157
MsaG000133 AT4G36260.1 52.500 160 61 5 13 162 92 246 3.07e-46 156
MsaG000133 AT2G21400.1 41.981 212 74 6 12 223 6 168 3.64e-46 151
MsaG000133 AT2G21400.3 41.981 212 74 6 12 223 7 169 3.83e-46 151
MsaG000133 AT2G18120.1 47.134 157 56 3 13 162 70 206 1.98e-41 140
MsaG000133 AT2G18120.2 48.026 152 60 2 13 162 70 204 6.93e-39 134
MsaG000133 AT5G12330.1 45.625 160 75 3 15 162 112 271 1.62e-37 133
MsaG000133 AT5G12330.2 45.625 160 75 3 15 162 112 271 1.62e-37 133
MsaG000133 AT5G12330.4 45.625 160 75 3 15 162 199 358 1.81e-37 135
MsaG000133 AT5G33210.2 60.870 92 32 2 13 104 15 102 2.32e-31 111
MsaG000133 AT5G33210.1 48.819 127 54 4 13 139 50 165 3.27e-31 113
MsaG000133 AT3G54430.1 35.185 162 81 4 11 168 37 178 1.12e-23 94.0
MsaG000133 AT5G12330.3 82.609 46 8 0 15 60 112 157 1.01e-21 90.1

Find 60 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGATCCCTCTGGAGCTTC+TGG 0.278538 1:+1796693 MsaT000133.1:CDS
AGCTCCTCTTAATGCTTTCA+TGG 0.295162 1:+1797555 MsaT000133.1:CDS
CACTCATGTCTTTAGGATCT+TGG 0.300540 1:-1797601 None:intergenic
TGAATTCACCGGCGGTGTTC+AGG 0.312818 1:+1797275 MsaT000133.1:CDS
TATATACCTGTGGTAGTAAT+AGG 0.332609 1:-1796738 None:intergenic
AGGTCTTCAAGTGGTGGTTT+AGG 0.350386 1:+1796435 MsaT000133.1:CDS
GGTGGTGGCGAGGGTTCTTC+CGG 0.367254 1:+1797439 MsaT000133.1:CDS
CCACCACCAGCAGCAGCATT+TGG 0.392576 1:-1797424 None:intergenic
CGTTCGCGGCGTTTAGCAGC+TGG 0.397352 1:-1796585 None:intergenic
CCTCTTAATGCTTTCATGGC+TGG 0.408461 1:+1797559 MsaT000133.1:CDS
GTGGACAATCTTTCTTAGCT+TGG 0.415642 1:-1796491 None:intergenic
TGAGTTGTTGCTGCCGTTCG+CGG 0.429437 1:-1796599 None:intergenic
GGCGAGGGTTCTTCCGGTGG+TGG 0.430350 1:+1797445 MsaT000133.1:CDS
TTGAAATTAGGGTTGGAGTT+GGG 0.437119 1:+1797235 MsaT000133.1:intron
AGTGGTGGTTTAGGTGGTGG+TGG 0.442865 1:+1796444 MsaT000133.1:CDS
GGTTCTTCCGGTGGTGGCAG+CGG 0.446891 1:+1797451 MsaT000133.1:CDS
TTCGCCACCGCTGCCACCAC+CGG 0.449406 1:-1797458 None:intergenic
CCAAATGCTGCTGCTGGTGG+TGG 0.457278 1:+1797424 MsaT000133.1:CDS
AAACTCATGTGAAAAGTACT+TGG 0.464467 1:+1796559 MsaT000133.1:CDS
TGCTGCTGCTGGTGGTGGCG+AGG 0.465038 1:+1797429 MsaT000133.1:CDS
TTGTATGATCAAGGACTTGA+AGG 0.474373 1:+1797388 MsaT000133.1:CDS
GAAAATCAAAATGATCCCTC+TGG 0.474468 1:+1796684 MsaT000133.1:CDS
TGTCTTTAGGATCTTGGAGG+TGG 0.488834 1:-1797595 None:intergenic
GCTGCTGCTGGTGGTGGCGA+GGG 0.489260 1:+1797430 MsaT000133.1:CDS
TCATGTCTTTAGGATCTTGG+AGG 0.489282 1:-1797598 None:intergenic
TATAGTGAAGAATACTCAAA+TGG 0.492700 1:-1797529 None:intergenic
TCAAGTGGTGGTTTAGGTGG+TGG 0.495997 1:+1796441 MsaT000133.1:CDS
ATGAAAGCATTAAGAGGAGC+TGG 0.499046 1:-1797553 None:intergenic
CCTGTGGTAGTAATAGGTAC+TGG 0.504192 1:-1796732 None:intergenic
ATTGTTAACATTTGTTGTGG+TGG 0.506186 1:-1797506 None:intergenic
TCTTCCGGTGGTGGCAGCGG+TGG 0.515802 1:+1797454 MsaT000133.1:CDS
TCTTCAAGTGGTGGTTTAGG+TGG 0.516785 1:+1796438 MsaT000133.1:CDS
AGTAATAGGTACTGGAGTAC+AGG 0.518362 1:-1796724 None:intergenic
CCAGCCATGAAAGCATTAAG+AGG 0.523927 1:-1797559 None:intergenic
TTTGAAATTAGGGTTGGAGT+TGG 0.534737 1:+1797234 MsaT000133.1:intron
GGTGGTGGCAGCGGTGGCGA+AGG 0.539736 1:+1797460 MsaT000133.1:CDS
TTTACACACCTGAACACCGC+CGG 0.552531 1:-1797283 None:intergenic
TCCACCGGAGCTGAATTCAC+CGG 0.555263 1:+1797264 MsaT000133.1:CDS
GAACTTGTTGCAAGAGTCGA+GGG 0.557726 1:+1796526 MsaT000133.1:CDS
CAGCTGCTAAACGCCGCGAA+CGG 0.557914 1:+1796586 MsaT000133.1:CDS
GTCTTTAGGATCTTGGAGGT+GGG 0.561171 1:-1797594 None:intergenic
CCAGTACCTATTACTACCAC+AGG 0.564967 1:+1796732 MsaT000133.1:CDS
CAAACTGCTGTGAACATAGG+AGG 0.565014 1:+1797346 MsaT000133.1:CDS
ACTAGTCCAAATGCTGCTGC+TGG 0.565581 1:+1797418 MsaT000133.1:CDS
GCCGGTGAATTCAGCTCCGG+TGG 0.569608 1:-1797265 None:intergenic
TATCAAACTGCTGTGAACAT+AGG 0.572121 1:+1797343 MsaT000133.1:CDS
GGTGGCGAGGGTTCTTCCGG+TGG 0.572206 1:+1797442 MsaT000133.1:CDS
ACCGCCGGTGAATTCAGCTC+CGG 0.574562 1:-1797268 None:intergenic
TAGAGAACCAGAAGCTCCAG+AGG 0.578477 1:-1796700 None:intergenic
AGTCCAAATGCTGCTGCTGG+TGG 0.584415 1:+1797421 MsaT000133.1:CDS
AACTCATGTGAAAAGTACTT+GGG 0.591492 1:+1796560 MsaT000133.1:CDS
TGGATTGTTAACATTTGTTG+TGG 0.600855 1:-1797509 None:intergenic
AGAACTTGTTGCAAGAGTCG+AGG 0.603647 1:+1796525 MsaT000133.1:CDS
GTGATGAGGTCTTCAAGTGG+TGG 0.621009 1:+1796429 None:intergenic
CAAGTTCTACATCTAAGATG+TGG 0.632750 1:-1796510 None:intergenic
GGAATGAACTGTCAAGATTG+TGG 0.658447 1:+1796465 MsaT000133.1:CDS
ACCGGAGCTGAATTCACCGG+CGG 0.668533 1:+1797267 MsaT000133.1:CDS
AGAGAACCAGAAGCTCCAGA+GGG 0.671479 1:-1796699 None:intergenic
GTGTGTAAAAGTGAGTGCAA+TGG 0.678715 1:+1797297 MsaT000133.1:CDS
ACAGTGATGAGGTCTTCAAG+TGG 0.705760 1:+1796426 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 1796432 1797612 1796432 ID=MsaG000133
Chr1 mRNA 1796432 1797612 1796432 ID=MsaT000133.1;Parent=MsaG000133
Chr1 exon 1796432 1796753 1796432 ID=MsaT000133.1.exon1;Parent=MsaT000133.1
Chr1 CDS 1796432 1796753 1796432 ID=cds.MsaT000133.1;Parent=MsaT000133.1
Chr1 exon 1797245 1797612 1797245 ID=MsaT000133.1.exon2;Parent=MsaT000133.1
Chr1 CDS 1797245 1797612 1797245 ID=cds.MsaT000133.1;Parent=MsaT000133.1
Gene Sequence

>MsaT000133.1

ATGAGGTCTTCAAGTGGTGGTTTAGGTGGTGGTGGAATGAACTGTCAAGATTGTGGTAACCAAGCTAAGAAAGATTGTCCACATCTTAGATGTAGAACTTGTTGCAAGAGTCGAGGGTTTCAGTGTCAAACTCATGTGAAAAGTACTTGGGTTCCAGCTGCTAAACGCCGCGAACGGCAGCAACAACTCACGTCGTTGCAGCAACAAAATCAGCAGCAGCAACAACAGTTTCACTCATCAAGAAGACACAGAGAAAATCAAAATGATCCCTCTGGAGCTTCTGGTTCTCTAACCTGTACTCCAGTACCTATTACTACCACAGGGTTGGAGTTGGGAAATTTTCCACCGGAGCTGAATTCACCGGCGGTGTTCAGGTGTGTAAAAGTGAGTGCAATGGATGCACAAGATGAACGTTATGCTTATCAAACTGCTGTGAACATAGGAGGAAGAGTTTTCAAGGGAATTTTGTATGATCAAGGACTTGAAGGTAGCTACACTAGTCCAAATGCTGCTGCTGGTGGTGGCGAGGGTTCTTCCGGTGGTGGCAGCGGTGGCGAAGGAAATCAACAAAATCTCATAACAACCACCACAACAAATGTTAACAATCCATTTGAGTATTCTTCACTATATCCAGCTCCTCTTAATGCTTTCATGGCTGGTACGCAATTCTTCCCACCTCCAAGATCCTAA
Protein sequence

>MsaT000133.1

MRSSSGGLGGGGMNCQDCGNQAKKDCPHLRCRTCCKSRGFQCQTHVKSTWVPAAKRRERQQQLTSLQQQNQQQQQQFHSSRRHRENQNDPSGASGSLTCTPVPITTTGLELGNFPPELNSPAVFRCVKVSAMDAQDERYAYQTAVNIGGRVFKGILYDQGLEGSYTSPNAAAGGGEGSSGGGSGGEGNQQNLITTTTTNVNNPFEYSSLYPAPLNAFMAGTQFFPPPRS*