Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG000181 | XP_013466107.1 | 100.000 | 93 | 0 | 0 | 1 | 93 | 1 | 93 | 1.48e-62 | 194 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG000181 | sp|F4JVB8|RADL1_ARATH | 58.621 | 87 | 36 | 0 | 7 | 93 | 14 | 100 | 5.99e-35 | 117 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG000181 | A0A072VEW4 | 100.000 | 93 | 0 | 0 | 1 | 93 | 1 | 93 | 7.08e-63 | 194 |
Gene ID | Type | Classification |
---|---|---|
MsaG000181 | TF | MYB-related |
Gene ID | Type | Classification |
---|
Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MsaG000181 | MsaG000706 | 0.825206 | 9.440976e-54 | 2.388292e-51 |
MsaG000181 | MsaG000968 | 0.837782 | 7.786405e-57 | 2.836684e-54 |
MsaG000181 | MsaG002496 | 0.825011 | 1.048762e-53 | 2.638997e-51 |
MsaG000181 | MsaG002558 | 0.808749 | 4.528933e-50 | 7.504697e-48 |
MsaG000181 | MsaG003688 | 0.805153 | 2.588252e-49 | 3.937962e-47 |
MsaG000181 | MsaG004003 | 0.819950 | 1.553519e-52 | 3.412266e-50 |
MsaG000181 | MsaG004084 | 0.821719 | 6.113863e-53 | 1.407312e-50 |
MsaG000181 | MsaG004654 | 0.807337 | 9.020928e-50 | 1.444866e-47 |
MsaG000181 | MsaG004943 | 0.800896 | 1.946031e-48 | 2.684811e-46 |
MsaG000181 | MsaG005078 | 0.801022 | 1.834113e-48 | 2.537683e-46 |
MsaG000181 | MsaG005420 | 0.807807 | 7.177998e-50 | 1.162706e-47 |
MsaG000181 | MsaG006085 | 0.829640 | 8.255007e-55 | 2.364611e-52 |
MsaG000181 | MsaG006940 | 0.802964 | 7.349245e-49 | 1.062856e-46 |
MsaG000181 | MsaG007030 | 0.810149 | 2.275510e-50 | 3.899973e-48 |
MsaG000181 | MsaG007279 | 0.801511 | 1.458031e-48 | 2.039801e-46 |
MsaG000181 | MsaG007300 | 0.800060 | 2.874576e-48 | 3.891891e-46 |
MsaG000181 | MsaG007403 | 0.814044 | 3.248031e-51 | 6.129450e-49 |
MsaG000181 | MsaG008003 | 0.826726 | 4.126518e-54 | 1.088922e-51 |
MsaG000181 | MsaG008239 | 0.829714 | 7.920898e-55 | 2.273642e-52 |
MsaG000181 | MsaG009075 | 0.819136 | 2.377374e-52 | 5.111152e-50 |
MsaG000181 | MsaG010637 | 0.837259 | 1.059170e-56 | 3.797132e-54 |
MsaG000181 | MsaG010854 | 0.812142 | 8.450511e-51 | 1.520732e-48 |
MsaG000181 | MsaG012389 | 0.807290 | 9.226230e-50 | 1.476104e-47 |
MsaG000181 | MsaG012814 | 0.801817 | 1.263083e-48 | 1.779412e-46 |
MsaG000181 | MsaG013974 | 0.819836 | 1.648883e-52 | 3.610618e-50 |
MsaG000181 | MsaG014465 | 0.836969 | 1.255298e-56 | 4.460825e-54 |
MsaG000181 | MsaG016329 | 0.807475 | 8.434527e-50 | 1.355412e-47 |
MsaG000181 | MsaG016664 | 0.802342 | 9.862375e-49 | 1.406158e-46 |
MsaG000181 | MsaG016816 | 0.831338 | 3.186609e-55 | 9.583175e-53 |
MsaG000181 | MsaG017119 | 0.805322 | 2.387028e-49 | 3.646063e-47 |
MsaG000181 | MsaG017187 | 0.863470 | 4.834155e-64 | 4.298052e-61 |
MsaG000181 | MsaG017221 | 0.831060 | 3.726143e-55 | 1.111612e-52 |
MsaG000181 | MsaG017290 | 0.832573 | 1.583918e-55 | 4.937847e-53 |
MsaG000181 | MsaG018897 | 0.823236 | 2.726638e-53 | 6.537492e-51 |
MsaG000181 | MsaG019118 | 0.847344 | 2.316247e-59 | 1.147388e-56 |
MsaG000181 | MsaG019122 | 0.830212 | 5.996499e-55 | 1.745970e-52 |
MsaG000181 | MsaG019187 | 0.805155 | 2.586462e-49 | 3.935438e-47 |
MsaG000181 | MsaG020476 | 0.821874 | 5.632743e-53 | 1.301877e-50 |
MsaG000181 | MsaG023641 | 0.829709 | 7.940750e-55 | 2.279066e-52 |
MsaG000181 | MsaG023950 | 0.834608 | 4.943750e-56 | 1.636676e-53 |
MsaG000181 | MsaG024615 | 0.822865 | 3.323642e-53 | 7.889660e-51 |
MsaG000181 | MsaG026754 | 0.850041 | 4.175764e-60 | 2.266213e-57 |
MsaG000181 | MsaG027725 | 0.811564 | 1.127929e-50 | 2.001209e-48 |
MsaG000181 | MsaG028192 | 0.849677 | 5.272768e-60 | 2.826607e-57 |
MsaG000181 | MsaG028620 | 0.887856 | 2.152612e-72 | 5.683590e-69 |
MsaG000181 | MsaG028754 | 0.807330 | 9.050457e-50 | 1.449353e-47 |
MsaG000181 | MsaG028802 | 0.804485 | 3.563273e-49 | 5.338002e-47 |
MsaG000181 | MsaG029147 | 0.864375 | 2.536007e-64 | 2.336862e-61 |
MsaG000181 | MsaG029580 | 0.803645 | 5.320025e-49 | 7.815824e-47 |
MsaG000181 | MsaG030196 | 0.814197 | 3.006265e-51 | 5.695253e-49 |
MsaG000181 | MsaG030231 | 0.843081 | 3.251355e-58 | 1.399772e-55 |
MsaG000181 | MsaG030681 | 0.804739 | 3.155897e-49 | 4.755604e-47 |
MsaG000181 | MsaG041072 | 0.827812 | 2.273486e-54 | 6.184491e-52 |
MsaG000181 | MsaG041299 | 0.814190 | 3.017384e-51 | 5.715202e-49 |
MsaG000181 | MsaG043695 | 0.848430 | 1.166462e-59 | 5.992510e-57 |
MsaG000181 | MsaG044611 | 0.807908 | 6.833548e-50 | 1.109637e-47 |
MsaG000181 | MsaG045630 | 0.816306 | 1.027174e-51 | 2.052535e-49 |
MsaG000181 | MsaG045811 | 0.811405 | 1.220483e-50 | 2.157032e-48 |
MsaG000181 | MsaG045822 | 0.809709 | 2.826968e-50 | 4.793805e-48 |
MsaG000181 | MsaG046643 | 0.802531 | 9.020067e-49 | 1.291626e-46 |
MsaG000181 | MsaG046771 | 0.826248 | 5.357044e-54 | 1.394929e-51 |
MsaG000181 | MsaG046865 | 0.820281 | 1.305927e-52 | 2.893749e-50 |
MsaG000181 | MsaG002348 | 0.800709 | 2.123450e-48 | 2.917448e-46 |
MsaG000181 | MsaG001756 | 0.811212 | 1.343335e-50 | 2.362974e-48 |
MsaG000181 | MsaG000001 | 0.809005 | 3.995782e-50 | 6.661898e-48 |
MsaG000181 | MsaG002578 | 0.818075 | 4.128204e-52 | 8.633149e-50 |
MsaG000181 | MsaG008811 | 0.858780 | 1.274115e-62 | 9.458804e-60 |
MsaG000181 | MsaG009124 | 0.802887 | 7.620882e-49 | 1.100201e-46 |
MsaG000181 | MsaG007870 | 0.810304 | 2.107284e-50 | 3.625440e-48 |
MsaG000181 | MsaG011899 | 0.821380 | 7.318912e-53 | 1.669533e-50 |
MsaG000181 | MsaG011821 | 0.820391 | 1.232676e-52 | 2.739328e-50 |
MsaG000181 | MsaG027840 | 0.853352 | 4.862877e-61 | 2.962253e-58 |
MsaG000181 | MsaG030561 | 0.804752 | 3.136343e-49 | 4.727679e-47 |
MsaG000181 | MsaG032991 | 0.840319 | 1.726830e-57 | 6.809117e-55 |
MsaG000181 | MsaG032445 | 0.824322 | 1.522073e-53 | 3.758686e-51 |
MsaG000181 | MsaG037527 | 0.807618 | 7.870095e-50 | 1.269015e-47 |
MsaG000181 | MsaG036408 | 0.842703 | 4.093747e-58 | 1.741264e-55 |
MsaG000181 | MsaG043306 | 0.837289 | 1.040296e-56 | 3.733010e-54 |
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG000181 | MtrunA17_Chr1g0155181 | 100.000 | 86 | 0 | 0 | 1 | 86 | 1 | 86 | 1.59e-61 | 181 |
MsaG000181 | MtrunA17_Chr3g0141601 | 73.404 | 94 | 21 | 2 | 1 | 93 | 1 | 91 | 8.20e-45 | 139 |
MsaG000181 | MtrunA17_Chr3g0144631 | 73.134 | 67 | 18 | 0 | 5 | 71 | 14 | 80 | 2.21e-34 | 112 |
MsaG000181 | MtrunA17_Chr3g0081631 | 57.447 | 94 | 33 | 3 | 1 | 93 | 4 | 91 | 2.51e-32 | 108 |
MsaG000181 | MtrunA17_Chr7g0255841 | 57.647 | 85 | 30 | 1 | 9 | 93 | 14 | 92 | 1.43e-30 | 103 |
MsaG000181 | MtrunA17_Chr3g0141641 | 47.253 | 91 | 45 | 1 | 6 | 93 | 2 | 92 | 9.66e-29 | 99.0 |
MsaG000181 | MtrunA17_Chr5g0404101 | 65.217 | 69 | 24 | 0 | 2 | 70 | 14 | 82 | 1.82e-28 | 98.6 |
MsaG000181 | MtrunA17_Chr6g0449111 | 58.571 | 70 | 29 | 0 | 2 | 71 | 5 | 74 | 3.15e-28 | 97.4 |
MsaG000181 | MtrunA17_Chr8g0373111 | 55.224 | 67 | 29 | 1 | 4 | 70 | 2 | 67 | 6.22e-26 | 91.3 |
MsaG000181 | MtrunA17_Chr8g0373121 | 55.224 | 67 | 29 | 1 | 4 | 70 | 2 | 67 | 6.22e-26 | 91.3 |
MsaG000181 | MtrunA17_Chr8g0373131 | 55.224 | 67 | 29 | 1 | 4 | 70 | 2 | 67 | 6.25e-26 | 91.3 |
MsaG000181 | MtrunA17_Chr8g0373101 | 56.338 | 71 | 30 | 1 | 4 | 74 | 2 | 71 | 4.04e-24 | 86.7 |
MsaG000181 | MtrunA17_Chr5g0404301 | 61.905 | 63 | 23 | 1 | 5 | 67 | 2 | 63 | 5.85e-24 | 86.3 |
MsaG000181 | MtrunA17_Chr1g0162081 | 50.602 | 83 | 41 | 0 | 1 | 83 | 6 | 88 | 2.61e-23 | 90.5 |
MsaG000181 | MtrunA17_Chr8g0364741 | 53.623 | 69 | 32 | 0 | 1 | 69 | 4 | 72 | 5.25e-22 | 83.2 |
MsaG000181 | MtrunA17_Chr8g0365011 | 57.576 | 66 | 28 | 0 | 4 | 69 | 5 | 70 | 1.73e-21 | 85.1 |
MsaG000181 | MtrunA17_Chr5g0436501 | 55.882 | 68 | 30 | 0 | 2 | 69 | 22 | 89 | 1.75e-21 | 85.1 |
MsaG000181 | MtrunA17_Chr3g0104071 | 52.113 | 71 | 34 | 0 | 4 | 74 | 40 | 110 | 2.85e-20 | 82.4 |
MsaG000181 | MtrunA17_Chr5g0429521 | 51.389 | 72 | 34 | 1 | 4 | 75 | 26 | 96 | 3.16e-20 | 82.4 |
MsaG000181 | MtrunA17_Chr7g0241761 | 50.000 | 72 | 36 | 0 | 2 | 73 | 30 | 101 | 1.35e-19 | 80.5 |
MsaG000181 | MtrunA17_Chr4g0062741 | 48.000 | 75 | 35 | 2 | 1 | 71 | 1 | 75 | 1.12e-17 | 75.5 |
MsaG000181 | MtrunA17_Chr1g0210471 | 42.254 | 71 | 41 | 0 | 7 | 77 | 24 | 94 | 1.84e-16 | 71.2 |
MsaG000181 | MtrunA17_Chr1g0194071 | 49.153 | 59 | 29 | 1 | 5 | 62 | 9 | 67 | 2.13e-14 | 62.8 |
MsaG000181 | MtrunA17_Chr7g0270711 | 47.619 | 63 | 32 | 1 | 3 | 64 | 7 | 69 | 1.80e-13 | 60.5 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG000181 | AT4G39250.1 | 58.621 | 87 | 36 | 0 | 7 | 93 | 14 | 100 | 6.09e-36 | 117 |
MsaG000181 | AT1G75250.1 | 72.464 | 69 | 19 | 0 | 2 | 70 | 7 | 75 | 2.12e-33 | 112 |
MsaG000181 | AT1G75250.2 | 72.464 | 69 | 19 | 0 | 2 | 70 | 7 | 75 | 2.52e-33 | 111 |
MsaG000181 | AT2G21650.1 | 55.682 | 88 | 38 | 1 | 7 | 93 | 14 | 101 | 1.28e-31 | 107 |
MsaG000181 | AT1G19510.1 | 69.841 | 63 | 19 | 0 | 10 | 72 | 15 | 77 | 1.60e-28 | 99.0 |
MsaG000181 | AT2G18328.1 | 65.079 | 63 | 22 | 0 | 10 | 72 | 14 | 76 | 5.40e-27 | 94.7 |
MsaG000181 | AT5G05790.2 | 52.632 | 76 | 35 | 1 | 4 | 79 | 28 | 102 | 2.50e-21 | 85.1 |
MsaG000181 | AT5G05790.1 | 52.632 | 76 | 35 | 1 | 4 | 79 | 28 | 102 | 2.50e-21 | 85.1 |
MsaG000181 | AT5G58900.1 | 53.030 | 66 | 31 | 0 | 4 | 69 | 31 | 96 | 2.66e-20 | 82.4 |
MsaG000181 | AT2G38090.1 | 55.556 | 63 | 28 | 0 | 7 | 69 | 29 | 91 | 3.30e-20 | 82.4 |
MsaG000181 | AT3G11280.2 | 49.315 | 73 | 37 | 0 | 7 | 79 | 33 | 105 | 3.41e-19 | 79.3 |
MsaG000181 | AT3G11280.1 | 49.315 | 73 | 37 | 0 | 7 | 79 | 33 | 105 | 3.41e-19 | 79.3 |
MsaG000181 | AT4G36570.1 | 72.727 | 44 | 12 | 0 | 7 | 50 | 12 | 55 | 5.09e-19 | 73.9 |
MsaG000181 | AT5G08520.1 | 43.590 | 78 | 44 | 0 | 1 | 78 | 6 | 83 | 1.16e-16 | 72.8 |
MsaG000181 | AT1G49010.1 | 43.836 | 73 | 38 | 1 | 2 | 71 | 3 | 75 | 8.26e-16 | 70.9 |
MsaG000181 | AT5G23650.1 | 46.377 | 69 | 37 | 0 | 1 | 69 | 7 | 75 | 5.00e-14 | 65.9 |
MsaG000181 | AT5G01200.1 | 43.662 | 71 | 39 | 1 | 5 | 74 | 28 | 98 | 1.60e-13 | 63.9 |
MsaG000181 | AT5G04760.1 | 40.299 | 67 | 39 | 1 | 3 | 69 | 2 | 67 | 6.58e-13 | 62.0 |
Find 25 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
TGTTGCAGCATTTCTGTATT+TGG | 0.079428 | 1:+2390448 | None:intergenic |
GCTAGCTTCTGCCACCTATC+TGG | 0.154946 | 1:+2390569 | None:intergenic |
AGAAATGCTGCAACAATTAA+AGG | 0.224298 | 1:-2390440 | MsaT000181.1:CDS |
CTAGCTTCTGCCACCTATCT+GGG | 0.322954 | 1:+2390570 | None:intergenic |
GTTGCAGCATTTCTGTATTT+GGG | 0.335288 | 1:+2390449 | None:intergenic |
CAAAAGATTTGAGAATGCTT+TGG | 0.369296 | 1:-2390612 | MsaT000181.1:CDS |
TGGGGTGTCCTTATCATAGA+TGG | 0.425086 | 1:+2390589 | None:intergenic |
TGAAGATTTGAAGCAAATTG+AGG | 0.447717 | 1:-2390489 | MsaT000181.1:CDS |
TGCTTTGGCCATCTATGATA+AGG | 0.453055 | 1:-2390597 | MsaT000181.1:CDS |
ATAGGTGGCAGAAGCTAGCA+AGG | 0.504876 | 1:-2390565 | MsaT000181.1:CDS |
CAGAAGCTAGCAAGGGCTGT+TGG | 0.521879 | 1:-2390557 | MsaT000181.1:CDS |
GCATTTCTGTATTTGGGCAA+AGG | 0.525051 | 1:+2390455 | None:intergenic |
ATGATAAGGACACCCCAGAT+AGG | 0.538561 | 1:-2390583 | MsaT000181.1:CDS |
TTCTGTCAATACTTCACTGA+AGG | 0.539972 | 1:+2389798 | None:intergenic |
AAGCTAGCAAGGGCTGTTGG+AGG | 0.552500 | 1:-2390554 | MsaT000181.1:CDS |
GAATGTCCTCAAGTTCAGCT+TGG | 0.559569 | 1:-2390649 | MsaT000181.1:CDS |
AGCTAGCAAGGGCTGTTGGA+GGG | 0.566835 | 1:-2390553 | MsaT000181.1:CDS |
AGACAGTTGAGGAAGTGAAA+AGG | 0.586038 | 1:-2390529 | MsaT000181.1:CDS |
TGTTGTCCAAGCTGAACTTG+AGG | 0.595382 | 1:+2390643 | None:intergenic |
GATTTGAAGCAAATTGAGGA+AGG | 0.610917 | 1:-2390485 | MsaT000181.1:CDS |
TAGCTTCTGCCACCTATCTG+GGG | 0.629096 | 1:+2390571 | None:intergenic |
TAGGTGGCAGAAGCTAGCAA+GGG | 0.655725 | 1:-2390564 | MsaT000181.1:CDS |
ATAAGGACACCCCAGATAGG+TGG | 0.660087 | 1:-2390580 | MsaT000181.1:CDS |
ATTACAGTGACGAAGAACAA+AGG | 0.662329 | 1:-2390412 | MsaT000181.1:intron |
TGTTGGAGGGAAGACAGTTG+AGG | 0.663833 | 1:-2390540 | MsaT000181.1:CDS |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
Chr1 | gene | 2389811 | 2390669 | 2389811 | ID=MsaG000181 |
Chr1 | mRNA | 2389811 | 2390669 | 2389811 | ID=MsaT000181.1;Parent=MsaG000181 |
Chr1 | exon | 2389811 | 2389835 | 2389811 | ID=MsaT000181.1.exon2;Parent=MsaT000181.1 |
Chr1 | CDS | 2389811 | 2389835 | 2389811 | ID=cds.MsaT000181.1;Parent=MsaT000181.1 |
Chr1 | exon | 2390413 | 2390669 | 2390413 | ID=MsaT000181.1.exon1;Parent=MsaT000181.1 |
Chr1 | CDS | 2390413 | 2390669 | 2390413 | ID=cds.MsaT000181.1;Parent=MsaT000181.1 |
Gene Sequence |
Protein sequence |