AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG000181


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000181 MtrunA17_Chr1g0155181 100.000 86 0 0 1 86 1 86 1.59e-61 181
MsaG000181 MtrunA17_Chr3g0141601 73.404 94 21 2 1 93 1 91 8.20e-45 139
MsaG000181 MtrunA17_Chr3g0144631 73.134 67 18 0 5 71 14 80 2.21e-34 112
MsaG000181 MtrunA17_Chr3g0081631 57.447 94 33 3 1 93 4 91 2.51e-32 108
MsaG000181 MtrunA17_Chr7g0255841 57.647 85 30 1 9 93 14 92 1.43e-30 103
MsaG000181 MtrunA17_Chr3g0141641 47.253 91 45 1 6 93 2 92 9.66e-29 99.0
MsaG000181 MtrunA17_Chr5g0404101 65.217 69 24 0 2 70 14 82 1.82e-28 98.6
MsaG000181 MtrunA17_Chr6g0449111 58.571 70 29 0 2 71 5 74 3.15e-28 97.4
MsaG000181 MtrunA17_Chr8g0373111 55.224 67 29 1 4 70 2 67 6.22e-26 91.3
MsaG000181 MtrunA17_Chr8g0373121 55.224 67 29 1 4 70 2 67 6.22e-26 91.3
MsaG000181 MtrunA17_Chr8g0373131 55.224 67 29 1 4 70 2 67 6.25e-26 91.3
MsaG000181 MtrunA17_Chr8g0373101 56.338 71 30 1 4 74 2 71 4.04e-24 86.7
MsaG000181 MtrunA17_Chr5g0404301 61.905 63 23 1 5 67 2 63 5.85e-24 86.3
MsaG000181 MtrunA17_Chr1g0162081 50.602 83 41 0 1 83 6 88 2.61e-23 90.5
MsaG000181 MtrunA17_Chr8g0364741 53.623 69 32 0 1 69 4 72 5.25e-22 83.2
MsaG000181 MtrunA17_Chr8g0365011 57.576 66 28 0 4 69 5 70 1.73e-21 85.1
MsaG000181 MtrunA17_Chr5g0436501 55.882 68 30 0 2 69 22 89 1.75e-21 85.1
MsaG000181 MtrunA17_Chr3g0104071 52.113 71 34 0 4 74 40 110 2.85e-20 82.4
MsaG000181 MtrunA17_Chr5g0429521 51.389 72 34 1 4 75 26 96 3.16e-20 82.4
MsaG000181 MtrunA17_Chr7g0241761 50.000 72 36 0 2 73 30 101 1.35e-19 80.5
MsaG000181 MtrunA17_Chr4g0062741 48.000 75 35 2 1 71 1 75 1.12e-17 75.5
MsaG000181 MtrunA17_Chr1g0210471 42.254 71 41 0 7 77 24 94 1.84e-16 71.2
MsaG000181 MtrunA17_Chr1g0194071 49.153 59 29 1 5 62 9 67 2.13e-14 62.8
MsaG000181 MtrunA17_Chr7g0270711 47.619 63 32 1 3 64 7 69 1.80e-13 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000181 AT4G39250.1 58.621 87 36 0 7 93 14 100 6.09e-36 117
MsaG000181 AT1G75250.1 72.464 69 19 0 2 70 7 75 2.12e-33 112
MsaG000181 AT1G75250.2 72.464 69 19 0 2 70 7 75 2.52e-33 111
MsaG000181 AT2G21650.1 55.682 88 38 1 7 93 14 101 1.28e-31 107
MsaG000181 AT1G19510.1 69.841 63 19 0 10 72 15 77 1.60e-28 99.0
MsaG000181 AT2G18328.1 65.079 63 22 0 10 72 14 76 5.40e-27 94.7
MsaG000181 AT5G05790.2 52.632 76 35 1 4 79 28 102 2.50e-21 85.1
MsaG000181 AT5G05790.1 52.632 76 35 1 4 79 28 102 2.50e-21 85.1
MsaG000181 AT5G58900.1 53.030 66 31 0 4 69 31 96 2.66e-20 82.4
MsaG000181 AT2G38090.1 55.556 63 28 0 7 69 29 91 3.30e-20 82.4
MsaG000181 AT3G11280.2 49.315 73 37 0 7 79 33 105 3.41e-19 79.3
MsaG000181 AT3G11280.1 49.315 73 37 0 7 79 33 105 3.41e-19 79.3
MsaG000181 AT4G36570.1 72.727 44 12 0 7 50 12 55 5.09e-19 73.9
MsaG000181 AT5G08520.1 43.590 78 44 0 1 78 6 83 1.16e-16 72.8
MsaG000181 AT1G49010.1 43.836 73 38 1 2 71 3 75 8.26e-16 70.9
MsaG000181 AT5G23650.1 46.377 69 37 0 1 69 7 75 5.00e-14 65.9
MsaG000181 AT5G01200.1 43.662 71 39 1 5 74 28 98 1.60e-13 63.9
MsaG000181 AT5G04760.1 40.299 67 39 1 3 69 2 67 6.58e-13 62.0

Find 25 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTGCAGCATTTCTGTATT+TGG 0.079428 1:+2390448 None:intergenic
GCTAGCTTCTGCCACCTATC+TGG 0.154946 1:+2390569 None:intergenic
AGAAATGCTGCAACAATTAA+AGG 0.224298 1:-2390440 MsaT000181.1:CDS
CTAGCTTCTGCCACCTATCT+GGG 0.322954 1:+2390570 None:intergenic
GTTGCAGCATTTCTGTATTT+GGG 0.335288 1:+2390449 None:intergenic
CAAAAGATTTGAGAATGCTT+TGG 0.369296 1:-2390612 MsaT000181.1:CDS
TGGGGTGTCCTTATCATAGA+TGG 0.425086 1:+2390589 None:intergenic
TGAAGATTTGAAGCAAATTG+AGG 0.447717 1:-2390489 MsaT000181.1:CDS
TGCTTTGGCCATCTATGATA+AGG 0.453055 1:-2390597 MsaT000181.1:CDS
ATAGGTGGCAGAAGCTAGCA+AGG 0.504876 1:-2390565 MsaT000181.1:CDS
CAGAAGCTAGCAAGGGCTGT+TGG 0.521879 1:-2390557 MsaT000181.1:CDS
GCATTTCTGTATTTGGGCAA+AGG 0.525051 1:+2390455 None:intergenic
ATGATAAGGACACCCCAGAT+AGG 0.538561 1:-2390583 MsaT000181.1:CDS
TTCTGTCAATACTTCACTGA+AGG 0.539972 1:+2389798 None:intergenic
AAGCTAGCAAGGGCTGTTGG+AGG 0.552500 1:-2390554 MsaT000181.1:CDS
GAATGTCCTCAAGTTCAGCT+TGG 0.559569 1:-2390649 MsaT000181.1:CDS
AGCTAGCAAGGGCTGTTGGA+GGG 0.566835 1:-2390553 MsaT000181.1:CDS
AGACAGTTGAGGAAGTGAAA+AGG 0.586038 1:-2390529 MsaT000181.1:CDS
TGTTGTCCAAGCTGAACTTG+AGG 0.595382 1:+2390643 None:intergenic
GATTTGAAGCAAATTGAGGA+AGG 0.610917 1:-2390485 MsaT000181.1:CDS
TAGCTTCTGCCACCTATCTG+GGG 0.629096 1:+2390571 None:intergenic
TAGGTGGCAGAAGCTAGCAA+GGG 0.655725 1:-2390564 MsaT000181.1:CDS
ATAAGGACACCCCAGATAGG+TGG 0.660087 1:-2390580 MsaT000181.1:CDS
ATTACAGTGACGAAGAACAA+AGG 0.662329 1:-2390412 MsaT000181.1:intron
TGTTGGAGGGAAGACAGTTG+AGG 0.663833 1:-2390540 MsaT000181.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 2389811 2390669 2389811 ID=MsaG000181
Chr1 mRNA 2389811 2390669 2389811 ID=MsaT000181.1;Parent=MsaG000181
Chr1 exon 2389811 2389835 2389811 ID=MsaT000181.1.exon2;Parent=MsaT000181.1
Chr1 CDS 2389811 2389835 2389811 ID=cds.MsaT000181.1;Parent=MsaT000181.1
Chr1 exon 2390413 2390669 2390413 ID=MsaT000181.1.exon1;Parent=MsaT000181.1
Chr1 CDS 2390413 2390669 2390413 ID=cds.MsaT000181.1;Parent=MsaT000181.1
Gene Sequence

>MsaT000181.1

ATGTCCTCAAGTTCAGCTTGGACAACAAAGCAAAACAAAAGATTTGAGAATGCTTTGGCCATCTATGATAAGGACACCCCAGATAGGTGGCAGAAGCTAGCAAGGGCTGTTGGAGGGAAGACAGTTGAGGAAGTGAAAAGGCATTATGAGATGTTAGTTGAAGATTTGAAGCAAATTGAGGAAGGTCATGTTCCTTTGCCCAAATACAGAAATGCTGCAACAATTAAAGGTTACAATTACAGTGACGAAGAACAAAGGTTGAAGTATCTAAGCCTTCAGTGA

Protein sequence

>MsaT000181.1

MSSSSAWTTKQNKRFENALAIYDKDTPDRWQKLARAVGGKTVEEVKRHYEMLVEDLKQIEEGHVPLPKYRNAATIKGYNYSDEEQRLKYLSLQ*