AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG000223


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000223 MtrunA17_Chr1g0154501 99.360 469 3 0 1 469 1 469 0.0 981
MsaG000223 MtrunA17_Chr8g0373781 58.192 531 159 9 1 468 1 531 0.0 576
MsaG000223 MtrunA17_Chr5g0436051 52.510 478 211 7 1 468 144 615 1.07e-171 496
MsaG000223 MtrunA17_Chr3g0112601 51.313 495 201 12 1 468 31 512 2.39e-161 466
MsaG000223 MtrunA17_Chr5g0403261 50.820 366 102 4 1 288 1 366 1.18e-123 365
MsaG000223 MtrunA17_Chr5g0403301 62.542 299 85 3 178 468 27 306 4.07e-122 358
MsaG000223 MtrunA17_Chr5g0403271 68.874 151 42 1 323 468 3 153 2.38e-62 199
MsaG000223 MtrunA17_Chr5g0403291 56.818 88 38 0 106 193 379 466 1.02e-31 127
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000223 AT1G19270.1 60.600 533 143 9 1 468 1 531 0.0 615
MsaG000223 AT1G19270.2 60.600 533 143 9 1 468 1 531 0.0 615
MsaG000223 AT4G36860.3 63.908 435 142 6 43 468 149 577 0.0 560
MsaG000223 AT4G36860.1 63.908 435 142 6 43 468 125 553 0.0 559
MsaG000223 AT2G39830.1 52.965 489 199 9 1 468 49 527 2.32e-171 492
MsaG000223 AT2G39830.3 53.125 480 203 7 1 468 49 518 6.92e-171 491
MsaG000223 AT2G39830.2 52.642 492 199 10 1 468 49 530 2.96e-169 487
MsaG000223 AT4G36860.2 64.329 328 108 4 43 365 125 448 9.77e-155 447
MsaG000223 AT2G39830.4 51.444 381 159 8 1 365 49 419 4.35e-130 384
MsaG000223 AT5G66610.3 46.556 421 202 8 54 462 15 424 2.42e-115 346
MsaG000223 AT5G66620.1 45.755 424 200 9 54 461 231 640 5.26e-115 352
MsaG000223 AT5G66610.1 45.824 443 218 9 35 462 116 551 6.92e-115 349
MsaG000223 AT5G66620.3 45.755 424 200 9 54 461 222 631 7.27e-115 352
MsaG000223 AT5G66620.4 45.755 424 200 9 54 461 222 631 7.27e-115 352
MsaG000223 AT5G66610.2 43.404 470 217 10 35 462 116 578 1.14e-109 337
MsaG000223 AT5G66630.1 46.995 366 167 10 110 461 346 698 2.43e-98 310
MsaG000223 AT5G17890.1 47.170 371 181 8 111 468 1240 1608 1.59e-94 313
MsaG000223 AT5G66640.1 39.062 448 230 12 39 461 9 438 1.29e-93 291
MsaG000223 AT5G66640.7 39.062 448 230 12 39 461 9 438 1.29e-93 291
MsaG000223 AT5G66640.5 39.062 448 231 12 39 461 40 470 3.61e-93 291
MsaG000223 AT5G66640.4 39.062 448 230 12 39 461 40 469 4.59e-93 290
MsaG000223 AT5G66640.3 45.511 323 156 6 158 461 10 331 1.77e-89 276
MsaG000223 AT5G66640.6 35.491 448 243 13 39 461 40 466 6.59e-77 248
MsaG000223 AT5G66640.2 35.491 448 242 13 39 461 40 465 8.20e-77 248
MsaG000223 AT5G66620.2 42.491 273 136 7 54 319 222 480 1.87e-66 222

Find 123 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCCTTATAGCATGCTTTA+TGG 0.147926 1:+2935076 None:intergenic
GGAGATATCTATACTTAATA+TGG 0.171031 1:+2932185 None:intergenic
TCAGACATTTCCCCTGTTTA+TGG 0.175438 1:-2932293 MsaT000223.1:CDS
TATTACTTGCCGTCAACTTC+AGG 0.242107 1:-2938121 MsaT000223.1:CDS
TATGGTGAAGGGTCCTTTCT+AGG 0.258048 1:+2932228 None:intergenic
AGAGCTTCCTTCCATCCTTA+TGG 0.265230 1:+2934248 None:intergenic
CATGTTATCATTTCCATGTT+TGG 0.271952 1:+2937177 None:intergenic
AGAAGGTATTTGTCAGGTTT+TGG 0.289094 1:-2932465 MsaT000223.1:CDS
AATATAGGGCACACCCATTC+TGG 0.293148 1:-2934479 MsaT000223.1:CDS
ATGTTATCATTTCCATGTTT+GGG 0.306036 1:+2937178 None:intergenic
AATAGAAGAAAGTCTAAATT+TGG 0.307251 1:-2937209 MsaT000223.1:CDS
TAAATTGCTTGGGTGCATTC+TGG 0.307599 1:-2935295 MsaT000223.1:CDS
TCAGCAAGTTCCTCTACTTT+TGG 0.312173 1:-2934125 MsaT000223.1:CDS
CTGCTGGTCTTATTGAATAT+AGG 0.329484 1:-2934494 MsaT000223.1:CDS
CATGCATGGCTGCGGCTAAC+AGG 0.330963 1:-2932709 MsaT000223.1:intron
TGCTGGTCTTATTGAATATA+GGG 0.342259 1:-2934493 MsaT000223.1:CDS
CTAAACCCATCTCCATAAAC+AGG 0.350411 1:+2932281 None:intergenic
GCACGGCAACGGAAGCATTC+AGG 0.350540 1:+2935269 None:intergenic
CTTCTTCTAATTGTGATTTG+TGG 0.366104 1:+2937251 None:intergenic
TTTCCATAAAGCATGCTATA+AGG 0.370626 1:-2935079 MsaT000223.1:CDS
TTCTTTCACAGATTACTTAC+CGG 0.370788 1:-2932757 MsaT000223.1:intron
GAGCTTCCTTCCATCCTTAT+GGG 0.381406 1:+2934249 None:intergenic
AACTACCCGTCACCTTGATA+TGG 0.383599 1:+2932210 None:intergenic
CTCTGAGAGGCAGAGTCCTC+TGG 0.389800 1:+2933667 None:intergenic
ACTGCAATTCTCATTCTATA+CGG 0.407169 1:-2933312 MsaT000223.1:CDS
CTATTTCCTGTGCCGAGTCT+AGG 0.412169 1:+2933381 None:intergenic
TGGCCGCTATCTAAATTGCT+TGG 0.419143 1:-2935306 MsaT000223.1:CDS
TAGACTTTCTTCTATTGCTC+TGG 0.423924 1:+2937216 None:intergenic
ATTTCCCCTGTTTATGGAGA+TGG 0.425346 1:-2932287 MsaT000223.1:CDS
TCGTGCTATTGCGTTGTCTT+TGG 0.426970 1:-2937945 MsaT000223.1:CDS
TGCACCTCATAATCAGAGAT+TGG 0.431937 1:+2935236 None:intergenic
ATAAGACCAGCAGGGTTTGT+TGG 0.433014 1:+2934504 None:intergenic
GGCCGCTATCTAAATTGCTT+GGG 0.433359 1:-2935305 MsaT000223.1:CDS
TGCTTGCAGACATCACATTT+CGG 0.436101 1:+2935045 None:intergenic
CAGCGCGCAGTCAGTTTGTA+TGG 0.443392 1:+2933342 None:intergenic
TTTCCCCTGTTTATGGAGAT+GGG 0.444481 1:-2932286 MsaT000223.1:CDS
TCCCCAAGCTTCTTCTCAAA+AGG 0.447372 1:+2932338 None:intergenic
CCTACCAATCTCTGATTATG+AGG 0.447976 1:-2935240 MsaT000223.1:intron
AGGCAGAGTCCTCTGGTCTC+AGG 0.449022 1:+2933674 None:intergenic
TGTTGAAGAAGGTATTTGTC+AGG 0.458972 1:-2932471 MsaT000223.1:CDS
CCCATCGGGAAATACTGAAT+TGG 0.458973 1:+2937148 None:intergenic
TGCAATACTGAGATTGGTTA+TGG 0.459883 1:-2935326 MsaT000223.1:CDS
CACTGAATGAAGCGAGGGAG+GGG 0.460388 1:-2934090 MsaT000223.1:CDS
GTGTCTCGATTCTGCTATCA+TGG 0.464850 1:-2934218 MsaT000223.1:CDS
TATAGCATGCTTTATGGAAA+GGG 0.466425 1:+2935082 None:intergenic
ATTACTTGCCGTCAACTTCA+GGG 0.481116 1:-2938120 MsaT000223.1:CDS
AGTATTTCCCGATGGGGTAC+AGG 0.482157 1:-2937141 MsaT000223.1:intron
AATAATGCTGGTTCCAGAAT+GGG 0.483891 1:+2934466 None:intergenic
CAATAATGCTGGTTCCAGAA+TGG 0.490304 1:+2934465 None:intergenic
TTATAGCATGCTTTATGGAA+AGG 0.491264 1:+2935081 None:intergenic
GACGGGATATATTGCCCATA+AGG 0.491327 1:-2934263 MsaT000223.1:CDS
CTTCAGTATATCAGCATGAA+GGG 0.492198 1:+2934177 None:intergenic
ACTCATACCTGTACCCCATC+GGG 0.492801 1:+2937134 None:intergenic
GGGAAATGTCTGATTCAATC+TGG 0.498038 1:+2932303 None:intergenic
ACTTCAGTATATCAGCATGA+AGG 0.502726 1:+2934176 None:intergenic
GAGATATCTATACTTAATAT+GGG 0.506138 1:+2932186 None:intergenic
CTATATTCAATAAGACCAGC+AGG 0.514177 1:+2934495 None:intergenic
TCTGAGCTTTCTTCTGCACC+AGG 0.519475 1:-2932425 MsaT000223.1:CDS
TTACATAATAGGATATGTGC+TGG 0.519801 1:-2935350 MsaT000223.1:intron
AACTCATACCTGTACCCCAT+CGG 0.520543 1:+2937133 None:intergenic
CCAATTCAGTATTTCCCGAT+GGG 0.521103 1:-2937148 MsaT000223.1:CDS
GGATATATTGCCCATAAGGA+TGG 0.523228 1:-2934259 MsaT000223.1:CDS
TGGTCTCAGGCATATGATAG+TGG 0.523864 1:+2933687 None:intergenic
ACAAGTACCCCTGAAGTTGA+CGG 0.527470 1:+2938112 None:intergenic
CTCATGAGCTAGAATCGATC+CGG 0.532124 1:+2932738 None:intergenic
GCACTGAATGAAGCGAGGGA+GGG 0.533144 1:-2934091 MsaT000223.1:CDS
CTAAGTCAAGATGTTGAAGA+AGG 0.540521 1:-2932482 MsaT000223.1:CDS
CCTCATAATCAGAGATTGGT+AGG 0.540834 1:+2935240 None:intergenic
TTCGCAAGTATGGCCTAGAA+AGG 0.547798 1:-2932241 MsaT000223.1:CDS
GCTGGCTGCAATACTGAGAT+TGG 0.548263 1:-2935332 MsaT000223.1:CDS
TACATGCAGCCACAAGGGAC+GGG 0.551584 1:-2934280 MsaT000223.1:intron
TATGGAGATGGGTTTAGAGC+CGG 0.552548 1:-2932275 MsaT000223.1:CDS
ACCAATTCAGTATTTCCCGA+TGG 0.560239 1:-2937149 MsaT000223.1:CDS
CTCATGAGATGATGCATGCA+TGG 0.562277 1:-2932723 MsaT000223.1:CDS
GCTGATGAAAAGTTGCTGCC+TGG 0.562692 1:+2932407 None:intergenic
CAGATTCCAACAAACCCTGC+TGG 0.563194 1:-2934510 MsaT000223.1:CDS
GCAATATATCCCGTCCCTTG+TGG 0.568174 1:+2934271 None:intergenic
CATGTGAAGGGCAATAATGC+TGG 0.569427 1:+2934454 None:intergenic
GTAGGTTGCAAGCACGGCAA+CGG 0.571499 1:+2935258 None:intergenic
GTGTTATCATGTTCATGTGA+AGG 0.573567 1:+2934441 None:intergenic
TCATTCTATACGGTCTTCCA+AGG 0.573571 1:-2933302 MsaT000223.1:intron
TTACATGCAGCCACAAGGGA+CGG 0.574092 1:-2934281 MsaT000223.1:intron
GCATCATATACATCTAAGAC+AGG 0.574513 1:-2932371 MsaT000223.1:CDS
TCGCTCACAGCTACAACAGC+GGG 0.574792 1:+2934416 None:intergenic
TCTGATCATAAGGTTTCTGA+AGG 0.579206 1:-2938169 MsaT000223.1:CDS
AAGGACCCTTCACCATATCA+AGG 0.581020 1:-2932222 MsaT000223.1:CDS
CCGTCACCTTGATATGGTGA+AGG 0.582188 1:+2932216 None:intergenic
TTGGAGTCACCTCCCAAACA+TGG 0.583689 1:-2937190 MsaT000223.1:CDS
CTGTTGTAGCTGTGAGCGAA+TGG 0.584516 1:-2934412 MsaT000223.1:CDS
TGCAGACATCACATTTCGGA+TGG 0.584761 1:+2935049 None:intergenic
TTCGCTCACAGCTACAACAG+CGG 0.587961 1:+2934415 None:intergenic
TGTCTTTCAACCAAAAGTAG+AGG 0.588772 1:+2934115 None:intergenic
ACTGAATGAAGCGAGGGAGG+GGG 0.590431 1:-2934089 MsaT000223.1:CDS
TCTCTTTACATGCAGCCACA+AGG 0.590442 1:-2934286 MsaT000223.1:intron
AGCACTGAATGAAGCGAGGG+AGG 0.591672 1:-2934092 MsaT000223.1:CDS
TGTTATCATGTTCATGTGAA+GGG 0.592374 1:+2934442 None:intergenic
TTATCATTTCCATGTTTGGG+AGG 0.593608 1:+2937181 None:intergenic
CTTCACCATATCAAGGTGAC+GGG 0.594001 1:-2932215 MsaT000223.1:CDS
ATATTGCCCATAAGGATGGA+AGG 0.594506 1:-2934255 MsaT000223.1:CDS
TTACTTGCCGTCAACTTCAG+GGG 0.595492 1:-2938119 MsaT000223.1:intron
CGTCACCTTGATATGGTGAA+GGG 0.595573 1:+2932217 None:intergenic
CCAAGCTTCTTCTCAAAAGG+AGG 0.596754 1:+2932341 None:intergenic
AGATGATGCATGCATGGCTG+CGG 0.598462 1:-2932717 MsaT000223.1:CDS
CCTTCACCATATCAAGGTGA+CGG 0.608624 1:-2932216 MsaT000223.1:CDS
AGATTGGTAGGTTGCAAGCA+CGG 0.611730 1:+2935252 None:intergenic
CTCTTTACATGCAGCCACAA+GGG 0.615445 1:-2934285 MsaT000223.1:intron
CACCCAAGCAATTTAGATAG+CGG 0.630015 1:+2935303 None:intergenic
AGAAGACCTAGACTCGGCAC+AGG 0.630642 1:-2933387 MsaT000223.1:CDS
ATATGATGCAGATGAGGATG+AGG 0.632065 1:+2932385 None:intergenic
GACAAGCACTGAATGAAGCG+AGG 0.639156 1:-2934096 MsaT000223.1:CDS
ACAAGCACTGAATGAAGCGA+GGG 0.649587 1:-2934095 MsaT000223.1:CDS
TAAACCCATCTCCATAAACA+GGG 0.651389 1:+2932282 None:intergenic
TTCAGTATATCAGCATGAAG+GGG 0.658616 1:+2934178 None:intergenic
AGATGTATATGATGCAGATG+AGG 0.664075 1:+2932379 None:intergenic
CAATTCAGTATTTCCCGATG+GGG 0.664407 1:-2937147 MsaT000223.1:CDS
TTGTAGCTGTGAGCGAATGG+AGG 0.666039 1:-2934409 MsaT000223.1:intron
CAGAGGACTCTGCCTCTCAG+AGG 0.667518 1:-2933666 MsaT000223.1:CDS
GAAAGGGTAATTTCCAGACG+TGG 0.673323 1:+2935098 None:intergenic
CAGTGCTGAGTTCCTCTGAG+AGG 0.678909 1:+2933654 None:intergenic
TATCATATGCCTGAGACCAG+AGG 0.682286 1:-2933683 MsaT000223.1:CDS
TATATTCAATAAGACCAGCA+GGG 0.688376 1:+2934496 None:intergenic
CTGATCATAAGGTTTCTGAA+GGG 0.689401 1:-2938168 MsaT000223.1:CDS
AAACCCATCTCCATAAACAG+GGG 0.693477 1:+2932283 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 2932199 2938221 2932199 ID=MsaG000223
Chr1 mRNA 2932199 2938221 2932199 ID=MsaT000223.1;Parent=MsaG000223
Chr1 exon 2932199 2932515 2932199 ID=MsaT000223.1.exon11;Parent=MsaT000223.1
Chr1 CDS 2932199 2932515 2932199 ID=cds.MsaT000223.1;Parent=MsaT000223.1
Chr1 exon 2932710 2932768 2932710 ID=MsaT000223.1.exon10;Parent=MsaT000223.1
Chr1 CDS 2932710 2932768 2932710 ID=cds.MsaT000223.1;Parent=MsaT000223.1
Chr1 exon 2933303 2933415 2933303 ID=MsaT000223.1.exon9;Parent=MsaT000223.1
Chr1 CDS 2933303 2933415 2933303 ID=cds.MsaT000223.1;Parent=MsaT000223.1
Chr1 exon 2933655 2933711 2933655 ID=MsaT000223.1.exon8;Parent=MsaT000223.1
Chr1 CDS 2933655 2933711 2933655 ID=cds.MsaT000223.1;Parent=MsaT000223.1
Chr1 exon 2934081 2934293 2934081 ID=MsaT000223.1.exon7;Parent=MsaT000223.1
Chr1 CDS 2934081 2934293 2934081 ID=cds.MsaT000223.1;Parent=MsaT000223.1
Chr1 exon 2934410 2934529 2934410 ID=MsaT000223.1.exon6;Parent=MsaT000223.1
Chr1 CDS 2934410 2934529 2934410 ID=cds.MsaT000223.1;Parent=MsaT000223.1
Chr1 exon 2935041 2935124 2935041 ID=MsaT000223.1.exon5;Parent=MsaT000223.1
Chr1 CDS 2935041 2935124 2935041 ID=cds.MsaT000223.1;Parent=MsaT000223.1
Chr1 exon 2935241 2935361 2935241 ID=MsaT000223.1.exon4;Parent=MsaT000223.1
Chr1 CDS 2935241 2935361 2935241 ID=cds.MsaT000223.1;Parent=MsaT000223.1
Chr1 exon 2937142 2937274 2937142 ID=MsaT000223.1.exon3;Parent=MsaT000223.1
Chr1 CDS 2937142 2937274 2937142 ID=cds.MsaT000223.1;Parent=MsaT000223.1
Chr1 exon 2937912 2938002 2937912 ID=MsaT000223.1.exon2;Parent=MsaT000223.1
Chr1 CDS 2937912 2938002 2937912 ID=cds.MsaT000223.1;Parent=MsaT000223.1
Chr1 exon 2938120 2938221 2938120 ID=MsaT000223.1.exon1;Parent=MsaT000223.1
Chr1 CDS 2938120 2938221 2938120 ID=cds.MsaT000223.1;Parent=MsaT000223.1
Gene Sequence

>MsaT000223.1

ATGGGTTGGCTTAGTAGAATTTTTAAAGGGTCTGATCATAAGGTTTCTGAAGGGCATTATTATAAAGATGATGCGAGTTATTACTTGCCGTCAACTTCAGGGGATGATTGGACTGAGAACGAGAATGAAGACATTGATCGTGCTATTGCGTTGTCTTTGGTAGAAGAAAATCAGAAAGTGAAAAATGTAAAAGACCACAAATCACAATTAGAAGAAGATGAACAACTTGCCAGAGCAATAGAAGAAAGTCTAAATTTGGAGTCACCTCCCAAACATGGAAATGATAACATGTATCAACCAATTCAGTATTTCCCGATGGGGTACAGGATATGTGCTGGCTGCAATACTGAGATTGGTTATGGCCGCTATCTAAATTGCTTGGGTGCATTCTGGCATCCTGAATGCTTCCGTTGCCGTGCTTGCAACCTACCAATCTCTGATTATGAGTTTTCCACGTCTGGAAATTACCCTTTCCATAAAGCATGCTATAAGGAAAGCTACCATCCGAAATGTGATGTCTGCAAGCACTTTATTCCAACAAACCCTGCTGGTCTTATTGAATATAGGGCACACCCATTCTGGAACCAGCATTATTGCCCTTCACATGAACATGATAACACTGCCCGCTGTTGTAGCTGTGAGCGAATGGAGCCACAAGGGACGGGATATATTGCCCATAAGGATGGAAGGAAGCTCTGCTTAGAGTGTCTCGATTCTGCTATCATGGATACTAATGAATGTCAGCCCCTTCATGCTGATATACTGAAGTTTTATGAAAGCATAAATATGAAATTGAATCAGCAAGTTCCTCTACTTTTGGTTGAAAGACAAGCACTGAATGAAGCGAGGGAGGGGGAAAAGAATGGCCACTATCATATGCCTGAGACCAGAGGACTCTGCCTCTCAGAGGAACTCAGCACTTTTTCAAGAAGACCTAGACTCGGCACAGGAAATAGAGACACGAGAGCACAGCCATACAAACTGACTGCGCGCTGTGATGTGACTGCAATTCTCATTCTATACGGTCTTCCAAGATTACTTACCGGATCGATTCTAGCTCATGAGATGATGCATGCATGGCTGCGGCTAACAGGTTTTCGGACTCTAAGTCAAGATGTTGAAGAAGGTATTTGTCAGGTTTTGGCTCACATGTGGTTGGAGTCTGAGCTTTCTTCTGCACCAGGCAGCAACTTTTCATCAGCCTCATCCTCATCTGCATCATATACATCTAAGACAGGTAAAAGACCTCCTTTTGAGAAGAAGCTTGGGGAGTTCTTCAAGCACCAGATTGAATCAGACATTTCCCCTGTTTATGGAGATGGGTTTAGAGCCGGTCAAAAAGCAGTTCGCAAGTATGGCCTAGAAAGGACCCTTCACCATATCAAGGTGACGGGTAGTTTCCCATATTAA

Protein sequence

>MsaT000223.1

MGWLSRIFKGSDHKVSEGHYYKDDASYYLPSTSGDDWTENENEDIDRAIALSLVEENQKVKNVKDHKSQLEEDEQLARAIEESLNLESPPKHGNDNMYQPIQYFPMGYRICAGCNTEIGYGRYLNCLGAFWHPECFRCRACNLPISDYEFSTSGNYPFHKACYKESYHPKCDVCKHFIPTNPAGLIEYRAHPFWNQHYCPSHEHDNTARCCSCERMEPQGTGYIAHKDGRKLCLECLDSAIMDTNECQPLHADILKFYESINMKLNQQVPLLLVERQALNEAREGEKNGHYHMPETRGLCLSEELSTFSRRPRLGTGNRDTRAQPYKLTARCDVTAILILYGLPRLLTGSILAHEMMHAWLRLTGFRTLSQDVEEGICQVLAHMWLESELSSAPGSNFSSASSSSASYTSKTGKRPPFEKKLGEFFKHQIESDISPVYGDGFRAGQKAVRKYGLERTLHHIKVTGSFPY*