AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG000237


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000237 MtrunA17_Chr1g0154261 96.981 265 2 1 1 265 1 259 0.0 523
MsaG000237 MtrunA17_Chr3g0109981 58.268 127 49 1 126 248 268 394 3.96e-40 145
MsaG000237 MtrunA17_Chr5g0410741 86.301 73 10 0 126 198 213 285 2.80e-37 137
MsaG000237 MtrunA17_Chr8g0366281 84.722 72 11 0 127 198 168 239 1.10e-35 132
MsaG000237 MtrunA17_Chr6g0479431 84.058 69 11 0 127 195 197 265 4.61e-34 127
MsaG000237 MtrunA17_Chr8g0379121 61.538 78 27 2 119 194 96 172 1.01e-22 97.4
MsaG000237 MtrunA17_Chr7g0243081 57.692 78 31 1 116 191 53 130 4.38e-22 94.7
MsaG000237 MtrunA17_Chr2g0320231 64.789 71 22 2 126 194 89 158 6.47e-21 92.0
MsaG000237 MtrunA17_Chr1g0210911 59.091 66 25 1 125 188 13 78 1.84e-20 90.1
MsaG000237 MtrunA17_Chr1g0162931 60.000 65 24 1 126 188 10 74 3.65e-20 88.6
MsaG000237 MtrunA17_Chr3g0095611 33.514 185 100 6 116 285 1 177 3.76e-19 84.0
MsaG000237 MtrunA17_Chr7g0261911 62.121 66 25 0 133 198 263 328 3.91e-19 87.4
MsaG000237 MtrunA17_Chr2g0288601 66.129 62 20 1 122 183 62 122 1.32e-18 80.5
MsaG000237 MtrunA17_Chr1g0190301 58.824 68 28 0 129 196 172 239 3.70e-18 84.0
MsaG000237 MtrunA17_Chr7g0274471 66.071 56 19 0 133 188 219 274 1.77e-17 82.4
MsaG000237 MtrunA17_Chr5g0409731 67.925 53 17 0 133 185 26 78 8.65e-17 79.3
MsaG000237 MtrunA17_Chr1g0197171 42.982 114 53 2 79 192 3 104 1.37e-16 78.6
MsaG000237 MtrunA17_Chr2g0283951 52.381 84 31 3 126 203 81 161 2.35e-16 79.0
MsaG000237 MtrunA17_Chr7g0258551 53.968 63 29 0 134 196 214 276 6.68e-16 77.4
MsaG000237 MtrunA17_Chr2g0290991 58.333 60 25 0 133 192 40 99 1.18e-15 76.3
MsaG000237 MtrunA17_Chr7g0254981 60.000 55 22 0 134 188 52 106 2.61e-15 74.7
MsaG000237 MtrunA17_Chr2g0291021 61.818 55 21 0 134 188 51 105 6.96e-15 73.9
MsaG000237 MtrunA17_Chr8g0356501 57.143 56 24 0 133 188 38 93 1.05e-14 73.6
MsaG000237 MtrunA17_Chr1g0175091 44.186 86 47 1 122 207 249 333 1.20e-14 73.9
MsaG000237 MtrunA17_Chr2g0310011 55.000 60 27 0 133 192 36 95 1.43e-14 73.2
MsaG000237 MtrunA17_Chr1g0175071 36.641 131 71 4 82 206 129 253 1.89e-14 72.8
MsaG000237 MtrunA17_Chr6g0461641 46.753 77 35 1 133 209 43 113 2.24e-14 73.2
MsaG000237 MtrunA17_Chr2g0321461 55.357 56 25 0 133 188 46 101 2.85e-14 72.8
MsaG000237 MtrunA17_Chr5g0417521 50.877 57 28 0 133 189 16 72 8.75e-14 71.2
MsaG000237 MtrunA17_Chr4g0042451 52.632 57 27 0 133 189 18 74 1.02e-13 70.9
MsaG000237 MtrunA17_Chr1g0175081 45.455 66 36 0 134 199 24 89 5.57e-13 64.3
MsaG000237 MtrunA17_Chr7g0242521 55.172 58 23 1 134 188 15 72 2.08e-12 65.9
MsaG000237 MtrunA17_Chr4g0062921 40.816 98 53 1 131 223 234 331 7.90e-12 65.5
MsaG000237 MtrunA17_Chr2g0304841 52.632 57 26 1 129 185 210 265 2.39e-11 64.3
MsaG000237 MtrunA17_Chr4g0045361 34.586 133 79 3 127 251 269 401 2.95e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000237 AT5G42630.1 48.148 270 99 9 11 272 17 253 7.75e-58 187
MsaG000237 AT5G42630.2 52.679 224 73 6 11 234 17 207 1.37e-54 177
MsaG000237 AT1G32240.2 61.290 124 27 3 75 198 177 279 4.13e-40 144
MsaG000237 AT1G32240.1 61.789 123 26 3 76 198 178 279 9.13e-40 144
MsaG000237 AT4G17695.1 82.192 73 13 0 126 198 158 230 4.12e-36 132
MsaG000237 AT5G16560.1 70.408 98 24 3 126 220 213 308 1.21e-34 130
MsaG000237 AT1G14600.1 57.500 80 32 1 113 190 2 81 1.04e-21 92.4
MsaG000237 AT2G42660.1 53.571 84 37 1 121 202 37 120 2.69e-21 91.3
MsaG000237 AT2G02060.2 60.870 69 25 1 119 185 14 82 9.98e-20 87.0
MsaG000237 AT2G02060.1 60.870 69 25 1 119 185 14 82 9.98e-20 87.0
MsaG000237 AT2G38300.1 58.824 68 28 0 131 198 53 120 1.13e-19 88.2
MsaG000237 AT4G04605.1 58.209 67 27 1 122 188 5 70 2.99e-19 85.1
MsaG000237 AT2G40260.1 54.217 83 37 1 117 199 68 149 4.45e-19 87.4
MsaG000237 AT4G04580.1 55.738 61 27 0 133 193 15 75 1.98e-17 78.6
MsaG000237 AT5G45580.1 66.038 53 18 0 133 185 8 60 6.30e-17 79.3
MsaG000237 AT4G04555.1 50.704 71 34 1 121 191 4 73 6.58e-17 75.5
MsaG000237 AT5G45580.2 66.038 53 18 0 133 185 24 76 7.77e-17 79.3
MsaG000237 AT5G29000.4 52.564 78 36 1 134 211 233 309 8.60e-17 79.7
MsaG000237 AT4G28610.1 65.455 55 19 0 134 188 227 281 2.22e-16 79.3
MsaG000237 AT3G04450.3 63.636 55 20 0 134 188 240 294 3.80e-16 77.8
MsaG000237 AT3G04450.4 63.636 55 20 0 134 188 200 254 5.68e-16 78.2
MsaG000237 AT3G04450.2 63.636 55 20 0 134 188 200 254 5.68e-16 78.2
MsaG000237 AT5G29000.1 65.455 55 19 0 134 188 190 244 5.69e-16 78.2
MsaG000237 AT5G29000.3 65.455 55 19 0 134 188 190 244 5.69e-16 78.2
MsaG000237 AT3G04450.1 63.636 55 20 0 134 188 240 294 6.40e-16 78.2
MsaG000237 AT5G29000.2 65.455 55 19 0 134 188 233 287 8.19e-16 77.8
MsaG000237 AT3G12730.1 55.385 65 29 0 133 197 24 88 9.62e-16 75.5
MsaG000237 AT5G06800.3 31.977 172 101 3 28 199 104 259 2.07e-15 75.5
MsaG000237 AT2G01060.1 46.591 88 34 2 134 208 17 104 2.43e-15 75.5
MsaG000237 AT3G24120.2 52.308 65 31 0 133 197 42 106 3.42e-15 75.1
MsaG000237 AT3G24120.1 52.308 65 31 0 133 197 42 106 3.58e-15 75.1
MsaG000237 AT5G06800.1 33.529 170 97 4 28 197 104 257 1.07e-14 74.3
MsaG000237 AT4G13640.1 58.491 53 22 0 133 185 38 90 1.28e-14 73.2
MsaG000237 AT4G13640.2 58.491 53 22 0 133 185 38 90 1.35e-14 73.2
MsaG000237 AT3G13040.3 63.636 55 20 0 134 188 243 297 1.49e-14 74.3
MsaG000237 AT3G13040.1 63.636 55 20 0 134 188 243 297 1.49e-14 74.3
MsaG000237 AT3G13040.2 63.636 55 20 0 134 188 243 297 1.49e-14 74.3
MsaG000237 AT1G79430.2 55.000 60 27 0 133 192 35 94 1.72e-14 73.6
MsaG000237 AT3G04030.1 41.748 103 52 4 133 230 46 145 2.22e-14 73.6
MsaG000237 AT3G04030.3 41.748 103 52 4 133 230 46 145 2.64e-14 73.2
MsaG000237 AT2G20400.1 63.636 55 20 0 134 188 233 287 3.19e-14 73.2
MsaG000237 AT2G20400.2 63.636 55 20 0 134 188 233 287 3.29e-14 73.2
MsaG000237 AT2G06020.1 45.455 77 42 0 129 205 83 159 3.34e-14 72.4
MsaG000237 AT5G06800.2 34.161 161 90 4 28 188 104 248 4.52e-14 72.4
MsaG000237 AT5G18240.2 53.571 56 26 0 133 188 46 101 1.18e-13 71.2
MsaG000237 AT5G18240.3 53.571 56 26 0 133 188 46 101 1.18e-13 71.2
MsaG000237 AT5G18240.5 53.571 56 26 0 133 188 46 101 1.29e-13 71.2
MsaG000237 AT5G18240.1 53.571 56 26 0 133 188 46 101 1.36e-13 71.2
MsaG000237 AT5G18240.4 53.571 56 26 0 133 188 46 101 1.36e-13 71.2
MsaG000237 AT1G69580.1 50.877 57 28 0 133 189 35 91 1.69e-12 67.4
MsaG000237 AT1G69580.2 50.877 57 28 0 133 189 35 91 1.72e-12 67.4
MsaG000237 AT3G04030.2 40.777 103 52 5 133 230 46 144 7.99e-12 65.9
MsaG000237 AT2G03500.1 50.000 64 32 0 122 185 223 286 1.92e-11 64.7
MsaG000237 AT1G32240.3 84.375 32 5 0 167 198 1 32 7.25e-11 59.7

Find 62 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTAACTCTAACACAGATTT+TGG 0.155756 1:+3130839 None:intergenic
TTTCCAGTTGAATTTGAATT+TGG 0.190843 1:-3125658 MsaT000237.1:CDS
GTTCACGCTGTTCAACTTCT+TGG 0.243560 1:-3131143 MsaT000237.1:CDS
TTTGTATTTGTCTCTATTGA+TGG 0.275353 1:+3128949 None:intergenic
AGAATACGTCAAACCAATTT+CGG 0.281284 1:+3128909 None:intergenic
AATTGCAAATGTTTATTTGC+AGG 0.301324 1:-3125626 MsaT000237.1:intron
TGAAGTGGTTGAGGTAAATT+TGG 0.306777 1:+3130210 None:intergenic
ACAACAATCTAAGGAATTAT+TGG 0.308927 1:+3117336 None:intergenic
GTTGAACAGCGTGAACAAAA+TGG 0.323557 1:+3131150 None:intergenic
CGAAATACAAGAGTCGTTAA+TGG 0.332750 1:-3131227 MsaT000237.1:CDS
GTTGTAGTCCATCTCATTCT+TGG 0.337367 1:+3131182 None:intergenic
CACTTTCAACCTCACATCTA+TGG 0.348750 1:-3131263 MsaT000237.1:CDS
TCATCCGCAACAACAATCTA+AGG 0.379645 1:+3117327 None:intergenic
TCTCCAAATTCAAATTCAAC+TGG 0.381097 1:+3125655 None:intergenic
TAAATTAATTCAGGACATCT+TGG 0.385795 1:-3128975 MsaT000237.1:intron
ACACGTCAAGAGTCACTTAC+AGG 0.413830 1:-3130792 MsaT000237.1:intron
GAAGACTTATGTTTAAAGAT+AGG 0.416511 1:+3131543 None:intergenic
ATTAATATTGTTCCTTTGCA+TGG 0.427276 1:-3130258 MsaT000237.1:CDS
CCAAAGCCTAATCTCAATGT+TGG 0.434197 1:+3131371 None:intergenic
TCAGAATCAGAAATATTAGA+AGG 0.435907 1:+3131521 None:intergenic
GTGATCATGGCAACGAAGTC+GGG 0.441836 1:+3117370 None:intergenic
GTGAAGACCACTGACAAATC+AGG 0.457613 1:-3130508 MsaT000237.1:CDS
GGCGGAGACGATTGTGATCA+TGG 0.460161 1:+3117357 None:intergenic
CACGCTGTTCAACTTCTTGG+TGG 0.460230 1:-3131140 MsaT000237.1:CDS
CCAACATTGAGATTAGGCTT+TGG 0.462514 1:-3131371 MsaT000237.1:CDS
TGAATTTGAATTTGGAGAGA+TGG 0.466148 1:-3125650 MsaT000237.1:CDS
AGTGACTCTTGACGTGTGCT+AGG 0.470529 1:+3130798 None:intergenic
TTGAACAGCGTGAACAAAAT+GGG 0.471767 1:+3131151 None:intergenic
TTTGACGTATTCTAATTTGA+AGG 0.475463 1:-3128899 MsaT000237.1:CDS
CAAAGCCTAATCTCAATGTT+GGG 0.475579 1:+3131372 None:intergenic
TGTGATCATGGCAACGAAGT+CGG 0.476549 1:+3117369 None:intergenic
CGTGAACAAAATGGGAATGA+AGG 0.478451 1:+3131159 None:intergenic
TTGTATTTCGCTTGAAATCG+CGG 0.491941 1:+3131240 None:intergenic
CATATATTATCATTATAAAG+TGG 0.498559 1:+3131479 None:intergenic
CTTATGTTTAAAGATAGGTC+TGG 0.507017 1:+3131548 None:intergenic
CGTGACCCAACATTGAGATT+AGG 0.510703 1:-3131377 MsaT000237.1:CDS
AATTCCTTAGATTGTTGTTG+CGG 0.525353 1:-3117331 MsaT000237.1:CDS
ACGTTGATGAGGATAGATGA+AGG 0.553867 1:+3131397 None:intergenic
ACCTGCTCCTGATTTGTCAG+TGG 0.561571 1:+3130501 None:intergenic
ACCACTGACAAATCAGGAGC+AGG 0.567981 1:-3130502 MsaT000237.1:intron
ACAATCTAAGGAATTATTGG+CGG 0.586448 1:+3117339 None:intergenic
GATAGGTCTGGTTCTAACAA+AGG 0.593492 1:+3131560 None:intergenic
ACTTCTGATTCAGCTAGTAG+TGG 0.595408 1:-3131443 MsaT000237.1:CDS
AACCACTTCAAAATTCACTT+CGG 0.596072 1:-3130197 MsaT000237.1:intron
AGATGTGAGGTTGAAAGTGT+TGG 0.598997 1:+3131267 None:intergenic
TTAGAGCTCCAAGAATGAGA+TGG 0.601023 1:-3131190 MsaT000237.1:CDS
GTGACTCTTGACGTGTGCTA+GGG 0.602003 1:+3130799 None:intergenic
ATTTCTGATTCTGAACCCAA+AGG 0.603069 1:-3131512 MsaT000237.1:CDS
TTTGAAGGAAAATGATACCA+TGG 0.610022 1:-3128884 MsaT000237.1:intron
CGTTGATGAGGATAGATGAA+GGG 0.610120 1:+3131398 None:intergenic
GTGAACAAAATGGGAATGAA+GGG 0.613934 1:+3131160 None:intergenic
CTTGCACTTGATGACGATGA+AGG 0.615738 1:+3131309 None:intergenic
CTTCATCGTCATCAAGTGCA+AGG 0.616119 1:-3131308 MsaT000237.1:CDS
AATGTTGGGTCACGTTGATG+AGG 0.616823 1:+3131386 None:intergenic
GAGGAATTAGCTCCATGCAA+AGG 0.631962 1:+3130246 None:intergenic
AATGGGATACTGCAGACACA+AGG 0.640188 1:-3130282 MsaT000237.1:CDS
AGGTGATGATTATGATGATG+TGG 0.640253 1:+3131329 None:intergenic
ATTTGGTCTCTCATCAGCTG+AGG 0.653148 1:+3130227 None:intergenic
GTTAGAGTTAATGAATGTGA+AGG 0.671020 1:-3130828 MsaT000237.1:CDS
ATGGGATACTGCAGACACAA+GGG 0.690336 1:-3130281 MsaT000237.1:CDS
AAATCGCGGCCATAGATGTG+AGG 0.691470 1:+3131254 None:intergenic
ATTCATTGTCGTAGCAAGGG+CGG 0.703314 1:-3117399 MsaT000237.1:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 3117327 3131600 3117327 ID=MsaG000237
Chr1 mRNA 3117327 3131600 3117327 ID=MsaT000237.1;Parent=MsaG000237
Chr1 exon 3117327 3117407 3117327 ID=MsaT000237.1.exon7;Parent=MsaT000237.1
Chr1 CDS 3117327 3117407 3117327 ID=cds.MsaT000237.1;Parent=MsaT000237.1
Chr1 exon 3125627 3125680 3125627 ID=MsaT000237.1.exon6;Parent=MsaT000237.1
Chr1 CDS 3125627 3125680 3125627 ID=cds.MsaT000237.1;Parent=MsaT000237.1
Chr1 exon 3128885 3128984 3128885 ID=MsaT000237.1.exon5;Parent=MsaT000237.1
Chr1 CDS 3128885 3128984 3128885 ID=cds.MsaT000237.1;Parent=MsaT000237.1
Chr1 exon 3130198 3130306 3130198 ID=MsaT000237.1.exon4;Parent=MsaT000237.1
Chr1 CDS 3130198 3130306 3130198 ID=cds.MsaT000237.1;Parent=MsaT000237.1
Chr1 exon 3130503 3130542 3130503 ID=MsaT000237.1.exon3;Parent=MsaT000237.1
Chr1 CDS 3130503 3130542 3130503 ID=cds.MsaT000237.1;Parent=MsaT000237.1
Chr1 exon 3130793 3130869 3130793 ID=MsaT000237.1.exon2;Parent=MsaT000237.1
Chr1 CDS 3130793 3130869 3130793 ID=cds.MsaT000237.1;Parent=MsaT000237.1
Chr1 exon 3131132 3131600 3131132 ID=MsaT000237.1.exon1;Parent=MsaT000237.1
Chr1 CDS 3131132 3131600 3131132 ID=cds.MsaT000237.1;Parent=MsaT000237.1
Gene Sequence

>MsaT000237.1

ATGCATACTTTATTTTCACCTTTGTTAGAACCAGACCTATCTTTAAACATAAGTCTTCCTTCTAATATTTCTGATTCTGAACCCAAAGGAATAACAAAACCACTTTATAATGATAATATATGTTCAATATCAACTACTTCTGATTCAGCTAGTAGTGGAAGTGAGTTAAGCCATGAAAATCCCTTCATCTATCCTCATCAACGTGACCCAACATTGAGATTAGGCTTTGGAAATTCAGATTTGATGAATCCACATCATCATAATCATCACCTTCATCGTCATCAAGTGCAAGGTGTATCAAGAAACTTTAACCAACACTTTCAACCTCACATCTATGGCCGCGATTTCAAGCGAAATACAAGAGTCGTTAATGGTGTTAAAAGAAGTGTTAGAGCTCCAAGAATGAGATGGACTACAACCCTTCATTCCCATTTTGTTCACGCTGTTCAACTTCTTGGTGGTCATGAAAGAGCAACACCAAAATCTGTGTTAGAGTTAATGAATGTGAAGGATCTAACCCTAGCACACGTCAAGAGTCACTTACAGATGTATAGAACAGTGAAGACCACTGACAAATCAGGAGCAGGTAATGGGATACTGCAGACACAAGGGATTAATATTGTTCCTTTGCATGGAGCTAATTCCTCAGCTGATGAGAGACCAAATTTACCTCAACCACTTCAAAATTCACTTCGGACATCTTGGCAACCATCAATAGAGACAAATACAAACAATACTGAAGAAAAATCCGAAATTGGTTTGACGTATTCTAATTTGAAGGAAAATGATACCATGTTTCCAGTTGAATTTGAATTTGGAGAGATGGTAATTGCAAATGTTTATTTGCAGCAAGGGCGGTACAATCCCGACTTCGTTGCCATGATCACAATCGTCTCCGCCAATAATTCCTTAGATTGTTGTTGCGGATGA

Protein sequence

>MsaT000237.1

MHTLFSPLLEPDLSLNISLPSNISDSEPKGITKPLYNDNICSISTTSDSASSGSELSHENPFIYPHQRDPTLRLGFGNSDLMNPHHHNHHLHRHQVQGVSRNFNQHFQPHIYGRDFKRNTRVVNGVKRSVRAPRMRWTTTLHSHFVHAVQLLGGHERATPKSVLELMNVKDLTLAHVKSHLQMYRTVKTTDKSGAGNGILQTQGINIVPLHGANSSADERPNLPQPLQNSLRTSWQPSIETNTNNTEEKSEIGLTYSNLKENDTMFPVEFEFGEMVIANVYLQQGRYNPDFVAMITIVSANNSLDCCCG*