AlfalfaGEDB | MsaG000238

M. sativa ssp. caerulea / MsaG000238

KEGG: -
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
PFAM: PF02362.24

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000238	XP_039688837.1	97.839	509	10	1	1	508	1	509	0.0	1036

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000238	sp\|Q8RYD1\|REM16_ARATH	52.817	142	64	3	4	144	3	142	4.26e-44	161
MsaG000238	sp\|Q8RYD1\|REM16_ARATH	27.727	220	130	5	216	410	104	319	5.30e-18	88.6

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000238	B7FNF2	97.642	509	11	1	1	508	1	509	0.0	1033

TFs/TRs:

Gene ID	Type	Classification
MsaG000238	TF	B3

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000238	MsaG003967	0.819187	2.316085e-52	4.986036e-50
MsaG000238	MsaG005341	0.838303	5.728040e-57	2.120577e-54
MsaG000238	MsaG005342	0.878624	5.070977e-69	8.588593e-66
MsaG000238	MsaG005646	0.826542	4.562999e-54	1.197933e-51
MsaG000238	MsaG005947	0.831708	2.585160e-55	7.860127e-53
MsaG000238	MsaG006590	0.802197	1.055687e-48	1.500152e-46
MsaG000238	MsaG007071	0.801581	1.411041e-48	1.977170e-46
MsaG000238	MsaG007277	0.800962	1.886253e-48	2.606283e-46
MsaG000238	MsaG008248	0.872271	7.411153e-67	9.461212e-64
MsaG000238	MsaG008264	0.830081	6.454038e-55	1.872205e-52
MsaG000238	MsaG012889	0.849248	6.934859e-60	3.663517e-57
MsaG000238	MsaG013630	0.845027	9.835661e-59	4.511537e-56
MsaG000238	MsaG014943	0.801482	1.478015e-48	2.066392e-46
MsaG000238	MsaG017395	0.825060	1.021632e-53	2.574201e-51
MsaG000238	MsaG017552	0.820628	1.088401e-52	2.433796e-50
MsaG000238	MsaG019110	0.810988	1.501715e-50	2.627049e-48
MsaG000238	MsaG019335	0.813153	5.091913e-51	9.396898e-49
MsaG000238	MsaG021053	0.815728	1.380771e-51	2.718804e-49
MsaG000238	MsaG021312	0.810635	1.788843e-50	3.102531e-48
MsaG000238	MsaG023223	0.831069	3.706922e-55	1.106161e-52
MsaG000238	MsaG023226	0.860503	3.883405e-63	3.078253e-60
MsaG000238	MsaG023229	0.853014	6.071590e-61	3.655058e-58
MsaG000238	MsaG023798	0.800675	2.157936e-48	2.962544e-46
MsaG000238	MsaG026283	0.808734	4.563589e-50	7.559224e-48
MsaG000238	MsaG027299	0.821510	6.830050e-53	1.563423e-50
MsaG000238	MsaG027304	0.871188	1.687468e-66	2.056380e-63
MsaG000238	MsaG028306	0.802152	1.078469e-48	1.530926e-46
MsaG000238	MsaG028493	0.803846	4.833694e-49	7.134593e-47
MsaG000238	MsaG029046	0.820044	1.479160e-52	3.257093e-50
MsaG000238	MsaG029478	0.874307	1.545456e-67	2.156086e-64
MsaG000238	MsaG029613	0.836483	1.666734e-56	5.837201e-54
MsaG000238	MsaG030517	0.843483	2.543353e-58	1.109188e-55
MsaG000238	MsaG032200	0.896127	1.122627e-75	4.601939e-72
MsaG000238	MsaG032674	0.826224	5.429327e-54	1.412782e-51
MsaG000238	MsaG033734	0.804220	4.044795e-49	6.022393e-47
MsaG000238	MsaG035155	0.820763	1.013619e-52	2.274682e-50
MsaG000238	MsaG036376	0.824981	1.066005e-53	2.680206e-51
MsaG000238	MsaG036394	0.833597	8.834021e-56	2.838114e-53
MsaG000238	MsaG037118	0.819602	1.863889e-52	4.056247e-50
MsaG000238	MsaG037122	0.828295	1.741178e-54	4.800989e-52
MsaG000238	MsaG037124	0.826542	4.562224e-54	1.197741e-51
MsaG000238	MsaG037236	0.800482	2.361228e-48	3.227452e-46
MsaG000238	MsaG039395	0.844746	1.169929e-58	5.316413e-56
MsaG000238	MsaG039908	0.844363	1.481309e-58	6.646795e-56
MsaG000238	MsaG039911	0.835660	2.690557e-56	9.192267e-54
MsaG000238	MsaG039804	0.804413	3.689220e-49	5.517453e-47
MsaG000238	MsaG039828	0.804788	3.083988e-49	4.652496e-47
MsaG000238	MsaG039847	0.809618	2.955945e-50	5.001665e-48
MsaG000238	MsaG039859	0.818386	3.512643e-52	7.405157e-50
MsaG000238	MsaG039867	0.825766	6.966525e-54	1.789880e-51
MsaG000238	MsaG039877	0.804355	3.792783e-49	5.664828e-47
MsaG000238	MsaG040017	0.824476	1.400583e-53	3.473091e-51
MsaG000238	MsaG040020	0.824216	1.611125e-53	3.966906e-51
MsaG000238	MsaG040310	0.811662	1.074082e-50	1.910249e-48
MsaG000238	MsaG040539	0.829867	7.271542e-55	2.096505e-52
MsaG000238	MsaG041592	0.868057	1.748493e-65	1.869603e-62
MsaG000238	MsaG041873	0.800385	2.470218e-48	3.369098e-46
MsaG000238	MsaG044665	0.815632	1.449691e-51	2.847672e-49
MsaG000238	MsaG045323	0.832068	2.109473e-55	6.481073e-53
MsaG000238	MsaG045894	0.876462	2.852597e-68	4.378437e-65
MsaG000238	MsaG045922	0.804146	4.190979e-49	6.229225e-47
MsaG000238	MsaG046004	0.828320	1.717872e-54	4.740057e-52
MsaG000238	MsaG046149	0.830275	5.787697e-55	1.688264e-52
MsaG000238	MsaG046214	0.806167	1.589151e-49	2.475953e-47
MsaG000238	MsaG001990	0.801329	1.588066e-48	2.212613e-46
MsaG000238	MsaG001943	0.831476	2.947551e-55	8.900258e-53
MsaG000238	MsaG002769	0.828979	1.192379e-54	3.351450e-52
MsaG000238	MsaG000819	0.870428	2.993661e-66	3.531855e-63
MsaG000238	MsaG009147	0.805431	2.264792e-49	3.468078e-47
MsaG000238	MsaG013354	0.815196	1.810773e-51	3.517830e-49
MsaG000238	MsaG014587	0.811621	1.096276e-50	1.947763e-48
MsaG000238	MsaG012349	0.806002	1.720606e-49	2.670201e-47
MsaG000238	MsaG012532	0.849205	7.129425e-60	3.760860e-57
MsaG000238	MsaG019886	0.838533	4.999131e-57	1.864126e-54
MsaG000238	MsaG020018	0.838897	4.029700e-57	1.519805e-54
MsaG000238	MsaG019073	0.819368	2.106374e-52	4.556276e-50
MsaG000238	MsaG026575	0.803282	6.320080e-49	9.207736e-47
MsaG000238	MsaG032920	0.853049	5.935269e-61	3.577210e-58
MsaG000238	MsaG033837	0.809530	3.087494e-50	5.213168e-48
MsaG000238	MsaG032451	0.810520	1.893417e-50	3.274740e-48
MsaG000238	MsaG031429	0.807088	1.018055e-49	1.620994e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000238	MtrunA17_Chr1g0154251	97.642	509	10	2	1	508	1	508	0.0	1030
MsaG000238	MtrunA17_Chr1g0154211	49.357	389	160	10	10	393	6	362	1.01e-110	334
MsaG000238	MtrunA17_Chr1g0154241	63.218	261	82	5	7	266	2	249	2.17e-108	323
MsaG000238	MtrunA17_Chr1g0154101	43.779	434	185	12	11	396	168	590	5.14e-96	309
MsaG000238	MtrunA17_Chr1g0154101	69.942	173	44	3	6	177	4	169	6.07e-73	247
MsaG000238	MtrunA17_Chr1g0154111	44.961	387	160	9	10	393	9	345	3.48e-94	298
MsaG000238	MtrunA17_Chr1g0154111	49.561	228	78	6	41	264	368	562	1.12e-54	193
MsaG000238	MtrunA17_Chr1g0154191	44.417	403	145	14	7	393	2	341	2.13e-88	276
MsaG000238	MtrunA17_Chr1g0154171	40.931	408	172	11	10	414	6	347	2.22e-81	257
MsaG000238	MtrunA17_Chr1g0154131	42.228	386	122	12	11	393	168	455	8.20e-78	251
MsaG000238	MtrunA17_Chr1g0154131	67.045	176	49	2	2	177	3	169	2.20e-70	232
MsaG000238	MtrunA17_Chr1g0154161	78.873	142	24	3	4	144	8	144	6.48e-72	231
MsaG000238	MtrunA17_Chr1g0154071	39.323	384	171	10	15	393	145	471	5.68e-69	229
MsaG000238	MtrunA17_Chr1g0154071	72.059	136	38	0	2	137	3	138	1.40e-62	212
MsaG000238	MtrunA17_Chr1g0154151	36.986	365	135	9	41	393	19	300	7.12e-60	200
MsaG000238	MtrunA17_Chr1g0154041	37.255	102	63	1	26	127	15	115	4.81e-15	77.8
MsaG000238	MtrunA17_Chr3g0131891	35.484	93	59	1	25	116	17	109	1.45e-14	75.1
MsaG000238	MtrunA17_Chr7g0233571	36.957	92	57	1	26	116	9	100	1.19e-13	70.5
MsaG000238	MtrunA17_Chr7g0233561	35.870	92	58	1	25	115	16	107	1.58e-13	71.6
MsaG000238	MtrunA17_Chr7g0233531	40.000	75	45	0	42	116	2	76	2.51e-12	67.4
MsaG000238	MtrunA17_Chr7g0233541	36.709	79	50	0	42	120	2	80	2.00e-11	65.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000238	AT4G33280.3	34.878	410	232	10	4	410	3	380	2.04e-68	225
MsaG000238	AT4G33280.2	48.780	164	81	3	4	166	3	164	5.72e-47	165
MsaG000238	AT4G33280.1	52.817	142	64	3	4	144	3	142	4.33e-45	161
MsaG000238	AT4G33280.1	27.727	220	130	5	216	410	104	319	5.39e-19	88.6
MsaG000238	AT3G18990.1	24.543	383	225	13	38	415	18	341	4.40e-23	100
MsaG000238	AT3G18990.2	24.538	379	222	13	38	411	18	337	1.43e-22	99.8
MsaG000238	AT4G34400.2	31.293	147	94	2	27	173	17	156	1.90e-18	87.8
MsaG000238	AT4G34400.1	31.293	147	94	2	27	173	17	156	1.90e-18	87.8
MsaG000238	AT3G06160.5	28.017	232	124	8	27	255	11	202	3.27e-16	80.1
MsaG000238	AT3G06160.4	28.017	232	124	8	27	255	11	202	3.27e-16	80.1
MsaG000238	AT3G06160.3	28.017	232	124	8	27	255	11	202	3.27e-16	80.1
MsaG000238	AT3G06160.1	27.155	232	126	7	27	255	26	217	3.62e-16	80.1
MsaG000238	AT3G06160.2	27.155	232	126	8	27	255	70	261	3.77e-16	80.5
MsaG000238	AT3G06220.1	33.000	100	65	2	27	126	11	108	3.18e-14	71.2
MsaG000238	AT1G49480.3	28.824	170	111	5	247	415	66	226	1.12e-12	68.2
MsaG000238	AT1G49480.1	28.824	170	111	5	247	415	66	226	1.12e-12	68.2
MsaG000238	AT1G49480.2	28.824	170	111	5	247	415	66	226	1.12e-12	68.2
MsaG000238	AT3G53310.1	37.500	80	50	0	37	116	21	100	3.05e-12	67.8
MsaG000238	AT5G57720.1	35.955	89	56	1	27	114	13	101	4.65e-11	64.3

Find 132 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGGTTTAGTATCCACTTATT+AGG	0.155011	1:+3147762	None:intergenic
CACATAATCCTTGAGGTATT+TGG	0.165984	1:+3146326	None:intergenic
CTCAGGAGAAAGCTCTTAAT+TGG	0.185662	1:-3149043	MsaT000238.1:CDS
CAATGGCCTCCAGTTCTGTT+TGG	0.239462	1:+3147415	None:intergenic
CTTGGGAAGAAGACATTTAT+TGG	0.243378	1:-3150543	MsaT000238.1:CDS
TGAAATGAATGAATTGAAAA+TGG	0.249343	1:+3150518	None:intergenic
GACATTGTTTGAAAATGTTT+TGG	0.262691	1:+3149688	None:intergenic
GTATATAGTTCCTGCGTCTT+TGG	0.290182	1:+3148086	None:intergenic
CTCAAGCCCCTCTTCTTTCC+TGG	0.311310	1:+3147486	None:intergenic
ATCCACCACCTTGTTCAGTT+TGG	0.318706	1:+3149399	None:intergenic
TGTGACAGGCCTTCTATTTG+AGG	0.325138	1:+3148064	None:intergenic
CGTAACAATGGAGGACTTAC+TGG	0.329858	1:-3147660	MsaT000238.1:CDS
AATGGCCTCCAGTTCTGTTT+GGG	0.333107	1:+3147416	None:intergenic
GGTTTAGTATCCACTTATTA+GGG	0.333478	1:+3147763	None:intergenic
TCCACCACCTTGTTCAGTTT+GGG	0.342784	1:+3149400	None:intergenic
TTGTCCGTTTACCAGTTGTT+TGG	0.357600	1:+3149135	None:intergenic
CCTGAAAATGTGGTCCTAAA+AGG	0.366161	1:-3149653	MsaT000238.1:CDS
TTCAGTTTGGGCGGCAGCCT+CGG	0.376086	1:+3149412	None:intergenic
TGTCCGTTTACCAGTTGTTT+GGG	0.377248	1:+3149136	None:intergenic
GGTGGAGTGACATGGAATAT+AGG	0.380908	1:-3149626	MsaT000238.1:CDS
TGCGTCTTTGGAGAGCCCTT+TGG	0.384532	1:+3148098	None:intergenic
GTACCTTCTGAAACTACTAA+TGG	0.385556	1:-3149251	MsaT000238.1:CDS
CACCGTTGTACTTAAAGAAC+AGG	0.391008	1:+3149519	None:intergenic
GGAGAGCCCTTTGGAGACGC+TGG	0.392912	1:+3148107	None:intergenic
ATGAACTCTTATCTCAAATA+CGG	0.420688	1:-3146374	MsaT000238.1:CDS
GGTCTTCCTCTCTTCTTACT+CGG	0.421285	1:+3149083	None:intergenic
CGGTGCTGCCCAAACAGAAC+TGG	0.423283	1:-3147424	MsaT000238.1:CDS
GTGGTGGGTGTAGCAGATCT+TGG	0.425709	1:-3150561	MsaT000238.1:CDS
CCCTTTGGAGACGCTGGGGC+AGG	0.428621	1:+3148113	None:intergenic
GAAGTCAATACTCCTTCTAA+TGG	0.429462	1:-3149338	MsaT000238.1:CDS
AACACACGTCAAACTCTTCA+AGG	0.430454	1:+3147607	None:intergenic
ACACAATTACGCACCTTCAA+TGG	0.435249	1:+3147398	None:intergenic
AACTATATACCTCAAATAGA+AGG	0.436916	1:-3148073	MsaT000238.1:CDS
GCATCAGACATTAATTGCTC+AGG	0.442937	1:+3149194	None:intergenic
GTGGAGTGACATGGAATATA+GGG	0.454700	1:-3149625	MsaT000238.1:CDS
CAATTGTTGAGCAAAATCAT+TGG	0.454715	1:+3150480	None:intergenic
CAGTTCTGTTTGGGCAGCAC+CGG	0.454969	1:+3147425	None:intergenic
AAAACCAGCTTAGATCTAGA+AGG	0.457413	1:-3146291	MsaT000238.1:CDS
GAGAGCCCTTTGGAGACGCT+GGG	0.458201	1:+3148108	None:intergenic
CGTCCCAAACAACTGGTAAA+CGG	0.458610	1:-3149139	MsaT000238.1:CDS
ACTGTTCCGTCCCAAACAAC+TGG	0.462388	1:-3149146	MsaT000238.1:CDS
CCTTCATCAACACCATTAGA+AGG	0.467182	1:+3149326	None:intergenic
AAGAGGGGCTTGAGTTCTAC+AGG	0.469641	1:-3147477	MsaT000238.1:CDS
TCCTGAGAATTAGACGTTCG+TGG	0.469709	1:+3149059	None:intergenic
ACCACGAACGTCTAATTCTC+AGG	0.472756	1:-3149060	MsaT000238.1:CDS
TCAGGAGAAAGCTCTTAATT+GGG	0.480968	1:-3149042	MsaT000238.1:CDS
GGAGAATGCACCGCAGTAAG+AGG	0.480989	1:+3147507	None:intergenic
AGGAGCTGAGACAGACGAGC+AGG	0.482943	1:-3146415	MsaT000238.1:CDS
ACAGACGCAACAGCATAAAC+AGG	0.486042	1:+3149107	None:intergenic
ACAGAACTGGAGGCCATTGA+AGG	0.495326	1:-3147411	MsaT000238.1:intron
TACGCATGGGTAAGAATGAA+TGG	0.495657	1:-3147710	MsaT000238.1:CDS
CTTACTGCGGTGCATTCTCC+AGG	0.496763	1:-3147504	MsaT000238.1:CDS
TGGGTAAGAATGAATGGCTT+GGG	0.497908	1:-3147704	MsaT000238.1:CDS
ATGGGTAAGAATGAATGGCT+TGG	0.510570	1:-3147705	MsaT000238.1:CDS
GTTTGGGACGGAACAGTTGT+TGG	0.513367	1:+3149152	None:intergenic
ACCTGCCCCAGCGTCTCCAA+AGG	0.514129	1:-3148114	MsaT000238.1:CDS
GTCTTCCTCTCTTCTTACTC+GGG	0.516995	1:+3149084	None:intergenic
GAAATGAATGAATTGAAAAT+GGG	0.517659	1:+3150519	None:intergenic
CCTTCTAATGGTGTTGATGA+AGG	0.519085	1:-3149326	MsaT000238.1:CDS
ACTAGTCATTCCCTAATAAG+TGG	0.519210	1:-3147773	MsaT000238.1:intron
GTTTCAGAAGGTACAGCAAA+TGG	0.520424	1:+3149260	None:intergenic
CTTCAAATGGGATTACAAAA+CGG	0.520715	1:-3150583	None:intergenic
GGTGCCTTCTAGATCTAAGC+TGG	0.524684	1:+3146287	None:intergenic
TGGTGGGTGTAGCAGATCTT+GGG	0.526446	1:-3150560	MsaT000238.1:CDS
CAACGGTGAATCGTTGTTTG+AGG	0.529531	1:-3149504	MsaT000238.1:CDS
AAAGCTCTTAATTGGGTGAC+TGG	0.530787	1:-3149035	MsaT000238.1:intron
ATATTCCATGTCACTCCACC+AGG	0.530935	1:+3149629	None:intergenic
ATGGGATTACAAAACGGTGG+TGG	0.534211	1:-3150577	MsaT000238.1:CDS
CCTGCCCCAGCGTCTCCAAA+GGG	0.534279	1:-3148113	MsaT000238.1:CDS
CAAATACCTCAAGGATTATG+TGG	0.537700	1:-3146325	MsaT000238.1:CDS
ATGTCATGCAAATGACCAGC+TGG	0.547589	1:+3147576	None:intergenic
TCAAGATGTGATATTACGCA+TGG	0.548312	1:-3147724	MsaT000238.1:CDS
CATCAGACATTAATTGCTCA+GGG	0.548758	1:+3149195	None:intergenic
CGTTTACCAGTTGTTTGGGA+CGG	0.549463	1:+3149140	None:intergenic
TGGCGAGAGTTTCTGTGAGA+AGG	0.555646	1:-3149468	MsaT000238.1:CDS
AAGGTCCTGGTGGAGTGACA+TGG	0.566287	1:-3149634	MsaT000238.1:CDS
TGTATACAAGAGATTCTTCG+TGG	0.566958	1:-3147958	MsaT000238.1:intron
AGTCGTTCTCTTTCAAAGAG+TGG	0.570224	1:+3149543	None:intergenic
AATCTCTTGTATACATGTGA+AGG	0.571018	1:+3147966	None:intergenic
CTTGAGACGATGGAGCAAGA+TGG	0.572879	1:+3147742	None:intergenic
TTTCCATTAGTAGTTTCAGA+AGG	0.573429	1:+3149248	None:intergenic
GAGTTCTACAGGAAAGAAGA+GGG	0.574907	1:-3147466	MsaT000238.1:CDS
AAAGAAGCTGCCTGAAAATG+TGG	0.583717	1:-3149663	MsaT000238.1:CDS
GTGTGTTTGTTCAAACCAGC+TGG	0.583771	1:-3147591	MsaT000238.1:CDS
GCTGCCGCCCAAACTGAACA+AGG	0.584723	1:-3149407	MsaT000238.1:CDS
GCATTCTCCAGGAAAGAAGA+GGG	0.592388	1:-3147493	MsaT000238.1:CDS
TTCCTGTTCTTTAAGTACAA+CGG	0.596255	1:-3149521	MsaT000238.1:CDS
AAAATCATCCAAATACCTCA+AGG	0.596480	1:-3146334	MsaT000238.1:CDS
TGAACTCTTATCTCAAATAC+GGG	0.597831	1:-3146373	MsaT000238.1:CDS
AAACAACTGGTAAACGGACA+AGG	0.598332	1:-3149133	MsaT000238.1:CDS
TGCATTCTCCAGGAAAGAAG+AGG	0.603183	1:-3147494	MsaT000238.1:CDS
TGCTGCCCAAACAGAACTGG+AGG	0.604957	1:-3147421	MsaT000238.1:CDS
AGTTACAAGCAAGCTCTCCT+CGG	0.605075	1:+3147995	None:intergenic
AAGGTACAGCAAATGGCACT+CGG	0.606956	1:+3149267	None:intergenic
CATAATATTCGTAACAATGG+AGG	0.607755	1:-3147669	MsaT000238.1:CDS
AGGTGGTGGATGTTCTAGCA+AGG	0.607880	1:-3149387	MsaT000238.1:CDS
GCCCAAACTGAACAAGGTGG+TGG	0.609494	1:-3149401	MsaT000238.1:CDS
AATGTGGTCCTAAAAGGTCC+TGG	0.611828	1:-3149647	MsaT000238.1:CDS
GTGGATGTTCTAGCAAGGGA+AGG	0.613993	1:-3149382	MsaT000238.1:CDS
TGAGTTCTACAGGAAAGAAG+AGG	0.614505	1:-3147467	MsaT000238.1:CDS
TGGGATTACAAAACGGTGGT+GGG	0.615464	1:-3150576	MsaT000238.1:CDS
AACAATGGAGGACTTACTGG+TGG	0.616719	1:-3147657	MsaT000238.1:CDS
TATCATAATATTCGTAACAA+TGG	0.617731	1:-3147672	MsaT000238.1:CDS
ACCACCTTGTTCAGTTTGGG+CGG	0.621987	1:+3149403	None:intergenic
GGTGGTGGATGTTCTAGCAA+GGG	0.623883	1:-3149386	MsaT000238.1:CDS
AAAGGGCTCTCCAAAGACGC+AGG	0.627367	1:-3148096	MsaT000238.1:CDS
CAAGATGTGATATTACGCAT+GGG	0.629761	1:-3147723	MsaT000238.1:CDS
AGACATTAATTGCTCAGGGG+TGG	0.633255	1:+3149199	None:intergenic
ATGGAGGACTTACTGGTGGG+TGG	0.638391	1:-3147653	MsaT000238.1:CDS
AATATCACATCTTGAGACGA+TGG	0.639490	1:+3147732	None:intergenic
GGAGATTACTCCTCTTACTG+CGG	0.639926	1:-3147517	MsaT000238.1:CDS
AGAGCCCTTTGGAGACGCTG+GGG	0.640012	1:+3148109	None:intergenic
TCGGAAATGTGGGCACGCCG+AGG	0.642864	1:-3149429	MsaT000238.1:CDS
CGAGAGTTTCTGTGAGAAGG+CGG	0.644557	1:-3149465	MsaT000238.1:CDS
GGAGTTAAGAGAACACTGAC+CGG	0.646401	1:-3147444	MsaT000238.1:CDS
TGGATGTTCTAGCAAGGGAA+GGG	0.650202	1:-3149381	MsaT000238.1:CDS
AAGGGACACAAACAATTCTG+TGG	0.651587	1:-3149363	MsaT000238.1:CDS
GGAGCTGAGACAGACGAGCA+GGG	0.652803	1:-3146414	MsaT000238.1:CDS
TTGGTACCACATAATCCTTG+AGG	0.659780	1:+3146319	None:intergenic
GGCTCAGGCAGCATGTACCG+AGG	0.663486	1:-3148012	MsaT000238.1:CDS
GTGGTCCTAAAAGGTCCTGG+TGG	0.664078	1:-3149644	MsaT000238.1:CDS
ATAGGGCTGACAAGTAGAGA+CGG	0.678435	1:-3149608	MsaT000238.1:CDS
ACAATGGAGGACTTACTGGT+GGG	0.682704	1:-3147656	MsaT000238.1:CDS
TCTGTCCCGAGTAAGAAGAG+AGG	0.684134	1:-3149089	MsaT000238.1:CDS
AGTTCTACAGGAAAGAAGAG+GGG	0.697073	1:-3147465	MsaT000238.1:CDS
CAAATGGGATTACAAAACGG+TGG	0.705155	1:-3150580	MsaT000238.1:CDS
CATTCTCCAGGAAAGAAGAG+GGG	0.708428	1:-3147492	MsaT000238.1:CDS
TGAGAATTAGACGTTCGTGG+TGG	0.713597	1:+3149062	None:intergenic
GGTGACGACTCAGCTAGCAG+AGG	0.724066	1:+3146266	None:intergenic
GCCGCCCAAACTGAACAAGG+TGG	0.731431	1:-3149404	MsaT000238.1:CDS
ATCAGACATTAATTGCTCAG+GGG	0.741591	1:+3149196	None:intergenic
CAACTGGTAAACGGACAAGG+AGG	0.752519	1:-3149130	MsaT000238.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	3146275	3150599	3146275	ID=MsaG000238
Chr1	mRNA	3146275	3150599	3146275	ID=MsaT000238.1;Parent=MsaG000238
Chr1	exon	3146275	3146435	3146275	ID=MsaT000238.1.exon5;Parent=MsaT000238.1
Chr1	CDS	3146275	3146435	3146275	ID=cds.MsaT000238.1;Parent=MsaT000238.1
Chr1	exon	3147412	3147790	3147412	ID=MsaT000238.1.exon4;Parent=MsaT000238.1
Chr1	CDS	3147412	3147790	3147412	ID=cds.MsaT000238.1;Parent=MsaT000238.1
Chr1	exon	3147959	3148143	3147959	ID=MsaT000238.1.exon3;Parent=MsaT000238.1
Chr1	CDS	3147959	3148143	3147959	ID=cds.MsaT000238.1;Parent=MsaT000238.1
Chr1	exon	3149036	3149717	3149036	ID=MsaT000238.1.exon2;Parent=MsaT000238.1
Chr1	CDS	3149036	3149717	3149036	ID=cds.MsaT000238.1;Parent=MsaT000238.1
Chr1	exon	3150480	3150599	3150480	ID=MsaT000238.1.exon1;Parent=MsaT000238.1
Chr1	CDS	3150480	3150599	3150480	ID=cds.MsaT000238.1;Parent=MsaT000238.1

Gene Sequence

>MsaT000238.1

ATGGGATTACAAAACGGTGGTGGGTGTAGCAGATCTTGGGAAGAAGACATTTATTGGACCCATTTTCAATTCATTCATTTCACTCAATTTCTTCGCTCCAATGATTTTGCTCAACAATTGGCACTTCCCAAAACATTTTCAAACAATGTCAAAAAGAAGCTGCCTGAAAATGTGGTCCTAAAAGGTCCTGGTGGAGTGACATGGAATATAGGGCTGACAAGTAGAGACGGCACATTTTACTTCACGCAAGGGTGGGAACAATTTGTTAAAGACCACTCTTTGAAAGAGAACGACTTCCTGTTCTTTAAGTACAACGGTGAATCGTTGTTTGAGGTTTTAATATTTGATGGCGAGAGTTTCTGTGAGAAGGCGGCAGCTTATTTTGTTCGGAAATGTGGGCACGCCGAGGCTGCCGCCCAAACTGAACAAGGTGGTGGATGTTCTAGCAAGGGAAGGGACACAAACAATTCTGTGGATGAAGTCAATACTCCTTCTAATGGTGTTGATGAAGGTGTTTCACATGAAAAATCTTTGCATCTTAACAGTATCCGAGTGCCATTTGCTGTACCTTCTGAAACTACTAATGGAAAGACTTCTAATGCAGCTGTTGAATCTGCCACCCCTGAGCAATTAATGTCTGATGCAGCAGTCACTGAGACTGAGCCAACAACTGTTCCGTCCCAAACAACTGGTAAACGGACAAGGAGGCCTGTTTATGCTGTTGCGTCTGTCCCGAGTAAGAAGAGAGGAAGACCACCACGAACGTCTAATTCTCAGGAGAAAGCTCTTAATTGGGTGACTGATGCTGAACCTGCCCCAGCGTCTCCAAAGGGCTCTCCAAAGACGCAGGAACTATATACCTCAAATAGAAGGCCTGTCACAAAAGATGAGATTGAAAAAACTCTTCAGGTGGCTCAGGCAGCATGTACCGAGGAGAGCTTGCTTGTAACTATGCGACCTTCACATGTATACAAGAGATTCTTCGTGTCATTCCCTAATAAGTGGATACTAAACCATCTTGCTCCATCGTCTCAAGATGTGATATTACGCATGGGTAAGAATGAATGGCTTGGGAAATACTGCTATCATAATATTCGTAACAATGGAGGACTTACTGGTGGGTGGAAGTATTTTGCGCTCGACAACAACCTTGAAGAGTTTGACGTGTGTTTGTTCAAACCAGCTGGTCATTTGCATGACATATTGATCTTAGAAATGAGCATTTTTAGAGTTGTTGAGGAGATTACTCCTCTTACTGCGGTGCATTCTCCAGGAAAGAAGAGGGGCTTGAGTTCTACAGGAAAGAAGAGGGGAGTTAAGAGAACACTGACCGGTGCTGCCCAAACAGAACTGGAGGCCATTGAAGGAGCTGAGACAGACGAGCAGGGACTGCAAGCTGAAGATGATGAACTCTTATCTCAAATACGGGCCAAAAGAAACATAGTAAAATCATCCAAATACCTCAAGGATTATGTGGTACCAAAGAAGAAAACCAGCTTAGATCTAGAAGGCACCTCTGCTAGCTGA

Protein sequence

>MsaT000238.1

MGLQNGGGCSRSWEEDIYWTHFQFIHFTQFLRSNDFAQQLALPKTFSNNVKKKLPENVVLKGPGGVTWNIGLTSRDGTFYFTQGWEQFVKDHSLKENDFLFFKYNGESLFEVLIFDGESFCEKAAAYFVRKCGHAEAAAQTEQGGGCSSKGRDTNNSVDEVNTPSNGVDEGVSHEKSLHLNSIRVPFAVPSETTNGKTSNAAVESATPEQLMSDAAVTETEPTTVPSQTTGKRTRRPVYAVASVPSKKRGRPPRTSNSQEKALNWVTDAEPAPASPKGSPKTQELYTSNRRPVTKDEIEKTLQVAQAACTEESLLVTMRPSHVYKRFFVSFPNKWILNHLAPSSQDVILRMGKNEWLGKYCYHNIRNNGGLTGGWKYFALDNNLEEFDVCLFKPAGHLHDILILEMSIFRVVEEITPLTAVHSPGKKRGLSSTGKKRGVKRTLTGAAQTELEAIEGAETDEQGLQAEDDELLSQIRAKRNIVKSSKYLKDYVVPKKKTSLDLEGTSAS*