AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG000244

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000244 MtrunA17_Chr1g0154101 94.550 844 39 3 1 842 1 839 0.0 1629
MsaG000244 MtrunA17_Chr1g0154131 59.934 604 108 8 1 602 1 472 0.0 688
MsaG000244 MtrunA17_Chr1g0154131 31.611 329 188 8 504 832 181 472 1.79e-34 138
MsaG000244 MtrunA17_Chr1g0154071 58.541 603 126 8 6 604 9 491 0.0 639
MsaG000244 MtrunA17_Chr1g0154071 62.275 167 58 2 2 168 134 295 1.11e-58 208
MsaG000244 MtrunA17_Chr1g0154071 67.857 140 44 1 159 297 3 142 3.34e-55 198
MsaG000244 MtrunA17_Chr1g0154071 30.347 346 221 9 501 834 154 491 6.47e-33 134
MsaG000244 MtrunA17_Chr1g0154041 78.701 385 81 1 458 842 209 592 0.0 616
MsaG000244 MtrunA17_Chr1g0154041 40.196 102 59 2 183 283 15 115 3.35e-16 82.8
MsaG000244 MtrunA17_Chr1g0154041 34.375 128 72 4 24 151 15 130 3.43e-14 76.6
MsaG000244 MtrunA17_Chr1g0154211 59.193 446 114 6 168 610 7 387 1.60e-174 509
MsaG000244 MtrunA17_Chr1g0154211 78.750 160 33 1 8 167 6 164 6.15e-83 271
MsaG000244 MtrunA17_Chr1g0154211 30.155 388 212 10 501 840 20 396 4.16e-41 155
MsaG000244 MtrunA17_Chr1g0154191 48.052 462 132 7 168 616 6 372 6.50e-133 401
MsaG000244 MtrunA17_Chr1g0154191 76.829 164 38 0 5 168 2 165 1.55e-82 269
MsaG000244 MtrunA17_Chr1g0154191 39.875 321 165 6 542 842 56 368 3.21e-59 206
MsaG000244 MtrunA17_Chr1g0154171 50.000 446 125 5 168 611 7 356 9.73e-129 390
MsaG000244 MtrunA17_Chr1g0154171 74.534 161 34 2 8 168 6 159 1.23e-74 248
MsaG000244 MtrunA17_Chr1g0154171 39.377 353 188 10 501 842 20 357 4.62e-56 197
MsaG000244 MtrunA17_Chr1g0154111 49.779 452 121 7 168 607 10 367 1.35e-128 398
MsaG000244 MtrunA17_Chr1g0154111 79.290 169 33 2 1 168 1 168 4.98e-85 283
MsaG000244 MtrunA17_Chr1g0154111 48.689 267 71 6 197 460 368 571 5.54e-60 214
MsaG000244 MtrunA17_Chr1g0154111 60.606 132 27 3 40 168 368 477 1.47e-38 152
MsaG000244 MtrunA17_Chr1g0154111 31.412 347 191 9 519 837 40 367 1.15e-34 140
MsaG000244 MtrunA17_Chr1g0154151 46.714 426 110 10 197 616 19 333 2.75e-104 325
MsaG000244 MtrunA17_Chr1g0154151 38.024 334 161 11 521 838 20 323 1.40e-51 184
MsaG000244 MtrunA17_Chr1g0154151 68.217 129 30 1 40 168 19 136 1.78e-50 180
MsaG000244 MtrunA17_Chr1g0154241 62.548 259 76 5 165 407 3 256 1.41e-99 310
MsaG000244 MtrunA17_Chr1g0154241 77.640 161 35 1 5 165 2 161 2.53e-84 270
MsaG000244 MtrunA17_Chr1g0154251 44.570 442 184 14 158 588 5 396 2.25e-96 310
MsaG000244 MtrunA17_Chr1g0154251 69.886 176 45 3 1 169 3 177 2.00e-74 252
MsaG000244 MtrunA17_Chr1g0154161 48.299 294 122 5 8 271 13 306 6.05e-76 250
MsaG000244 MtrunA17_Chr1g0154161 81.343 134 23 1 168 299 14 147 3.64e-69 231
MsaG000244 MtrunA17_Chr1g0154161 38.484 343 162 11 501 842 27 321 1.30e-53 189
MsaG000244 MtrunA17_Chr1g0154161 48.241 199 85 2 414 611 139 320 8.56e-49 175
MsaG000244 MtrunA17_Chr1g0154121 70.238 84 23 1 529 610 3 86 2.78e-32 120
MsaG000244 MtrunA17_Chr1g0154121 47.619 84 43 1 757 839 2 85 3.35e-19 83.6
MsaG000244 MtrunA17_Chr6g0464121 60.714 84 31 2 523 604 113 196 1.31e-24 105
MsaG000244 MtrunA17_Chr4g0033961 24.891 458 295 14 187 639 10 423 5.28e-18 87.4
MsaG000244 MtrunA17_Chr7g0233561 25.352 284 161 9 23 258 16 296 2.41e-16 81.3
MsaG000244 MtrunA17_Chr7g0233561 33.663 101 65 2 184 283 19 118 1.48e-12 69.7
MsaG000244 MtrunA17_Chr7g0233561 42.574 101 53 2 492 587 199 299 1.08e-11 67.0
MsaG000244 MtrunA17_Chr7g0233571 32.468 154 87 3 195 332 23 175 1.64e-14 73.6
MsaG000244 MtrunA17_Chr7g0233571 35.789 95 61 0 20 114 5 99 5.04e-13 69.3
MsaG000244 MtrunA17_Chr3g0131891 35.870 92 59 0 23 114 17 108 2.25e-13 72.4
MsaG000244 MtrunA17_Chr3g0131891 30.061 163 90 4 177 327 17 167 1.05e-11 67.4
MsaG000244 MtrunA17_Chr5g0419201 32.456 114 74 1 193 306 27 137 4.48e-12 65.9
MsaG000244 MtrunA17_Chr5g0419201 34.444 90 59 0 25 114 16 105 5.63e-11 62.8
MsaG000244 MtrunA17_Chr1g0162441 39.080 87 52 1 187 272 11 97 4.79e-12 68.9
MsaG000244 MtrunA17_Chr7g0233531 37.333 75 47 0 198 272 2 76 9.60e-12 66.6
MsaG000244 MtrunA17_Chr7g0233531 39.189 74 45 0 41 114 2 75 8.98e-11 63.5
MsaG000244 MtrunA17_Chr7g0233541 35.443 79 51 0 198 276 2 80 2.59e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000244 AT4G33280.1 35.144 313 152 7 11 274 11 321 1.00e-42 159
MsaG000244 AT4G33280.1 49.664 149 69 3 170 314 11 157 3.17e-40 152
MsaG000244 AT4G33280.2 55.385 130 55 2 11 139 11 138 9.89e-41 152
MsaG000244 AT4G33280.2 52.344 128 60 1 170 296 11 138 3.34e-40 150
MsaG000244 AT4G33280.3 55.385 130 55 2 11 139 11 138 9.30e-40 152
MsaG000244 AT4G33280.3 52.344 128 60 1 170 296 11 138 2.70e-39 150
MsaG000244 AT4G34400.2 41.667 84 49 0 196 279 29 112 3.53e-17 85.1
MsaG000244 AT4G34400.2 38.372 86 53 0 39 124 29 114 5.11e-17 84.7
MsaG000244 AT4G34400.1 41.667 84 49 0 196 279 29 112 3.53e-17 85.1
MsaG000244 AT4G34400.1 38.372 86 53 0 39 124 29 114 5.11e-17 84.7
MsaG000244 AT3G06220.1 34.524 84 54 1 196 279 23 105 2.06e-14 72.4
MsaG000244 AT3G06220.1 29.464 112 71 2 39 150 23 126 3.45e-14 72.0
MsaG000244 AT3G18990.1 38.298 94 56 1 22 115 5 96 1.08e-12 70.9
MsaG000244 AT3G18990.1 36.250 80 51 0 193 272 17 96 3.13e-11 66.2
MsaG000244 AT3G18990.2 38.298 94 56 1 22 115 5 96 1.14e-12 70.9
MsaG000244 AT3G18990.2 36.250 80 51 0 193 272 17 96 3.90e-11 66.2
MsaG000244 AT5G66980.1 27.106 273 135 11 37 250 21 288 1.48e-12 70.1
MsaG000244 AT5G66980.1 39.216 102 57 2 184 281 11 111 1.98e-12 69.7
MsaG000244 AT3G06160.1 32.955 88 59 0 184 271 26 113 2.96e-12 69.3
MsaG000244 AT3G06160.1 34.615 78 51 0 37 114 36 113 4.41e-12 68.9
MsaG000244 AT5G60130.5 38.614 101 57 2 184 279 15 115 3.61e-12 67.8
MsaG000244 AT5G60130.5 40.449 89 48 2 39 122 27 115 4.92e-12 67.4
MsaG000244 AT3G06160.5 32.955 88 59 0 184 271 11 98 4.02e-12 68.6
MsaG000244 AT3G06160.5 28.689 122 87 0 37 158 21 142 4.49e-12 68.6
MsaG000244 AT3G06160.4 32.955 88 59 0 184 271 11 98 4.02e-12 68.6
MsaG000244 AT3G06160.4 28.689 122 87 0 37 158 21 142 4.49e-12 68.6
MsaG000244 AT3G06160.3 32.955 88 59 0 184 271 11 98 4.02e-12 68.6
MsaG000244 AT3G06160.3 28.689 122 87 0 37 158 21 142 4.49e-12 68.6
MsaG000244 AT3G06160.2 32.955 88 59 0 184 271 70 157 4.52e-12 69.3
MsaG000244 AT3G06160.2 28.689 122 87 0 37 158 80 201 6.63e-12 68.6
MsaG000244 AT5G60130.2 38.614 101 57 2 184 279 15 115 6.61e-12 67.8
MsaG000244 AT5G60130.2 40.449 89 48 2 39 122 27 115 9.98e-12 67.4
MsaG000244 AT5G60130.3 40.449 89 48 2 39 122 1 89 8.70e-12 67.4
MsaG000244 AT5G60130.4 38.614 101 57 2 184 279 12 112 1.06e-11 67.4
MsaG000244 AT5G60130.4 40.449 89 48 2 39 122 24 112 1.37e-11 67.0
MsaG000244 AT5G60130.1 38.614 101 57 2 184 279 15 115 1.11e-11 67.4
MsaG000244 AT5G60130.1 40.449 89 48 2 39 122 27 115 1.40e-11 67.0
MsaG000244 AT1G49475.1 39.535 86 51 1 187 272 39 123 6.01e-11 62.8
MsaG000244 AT1G49475.1 34.444 90 58 1 25 114 34 122 6.74e-11 62.8
MsaG000244 AT3G53310.1 33.333 96 56 1 185 272 5 100 8.58e-11 64.3

Find 171 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACACCACCGGGACCTTTA+AGG 0.159160 1:+3258451 None:intergenic
GACTTCTTCAACAGAATTAT+TGG 0.172022 1:+3259550 None:intergenic
CAACTCCACTAGGACCTTTA+AGG 0.178570 1:+3259813 None:intergenic
GTGATGCACGTTCGTATATC+TGG 0.186351 1:-3253908 MsaT000244.1:CDS
CGTGATAATGACATCAATTT+TGG 0.202243 1:-3253965 MsaT000244.1:CDS
TGAAATGAATGAACTGAAAA+TGG 0.212602 1:+3261275 None:intergenic
TAAATTGTCTGAAAATGTTT+TGG 0.223184 1:+3259862 None:intergenic
CCATCCATTTGTGAAGTAAA+TGG 0.223284 1:+3259757 None:intergenic
CGGTGGTGGTTTGAAGAAAT+TGG 0.224658 1:+3261251 None:intergenic
TAACAGACTTCTTGGTCTTT+TGG 0.234122 1:+3257761 None:intergenic
CCAGAAAATGTGACCCTTAA+AGG 0.236805 1:-3258465 MsaT000244.1:CDS
GGTGGTGTTGCATGGAAAAT+AGG 0.237795 1:-3258438 MsaT000244.1:CDS
CCAGACAATGTGACCCTTAA+AGG 0.245088 1:-3259827 MsaT000244.1:CDS
AAGTTGTTGCTTAAAATCTT+TGG 0.248017 1:+3259421 None:intergenic
AACTTCATTAACAGACTTCT+TGG 0.252529 1:+3257753 None:intergenic
CTGCCTTCCCACTTCTATTC+AGG 0.256160 1:+3256042 None:intergenic
GAAGGTCTTGATCATCAAAA+TGG 0.258424 1:-3253875 MsaT000244.1:CDS
TGAAATGGATGAACTGAAAA+TGG 0.274129 1:+3259456 None:intergenic
TGTTGAACGTCCACGTATTC+TGG 0.284407 1:-3255898 MsaT000244.1:intron
CTTGGGAGAAAGAGATTTAC+TGG 0.286889 1:-3261300 MsaT000244.1:CDS
TGGTTTATATGAGAAATTTC+AGG 0.307206 1:-3258136 MsaT000244.1:CDS
CCTACCAACCAACCGGAAAA+AGG 0.307983 1:-3257975 MsaT000244.1:CDS
CTTGCATGCAATCTAGTTCT+AGG 0.336549 1:+3255088 None:intergenic
TTTCAGGATCTTAACAATAT+AGG 0.337128 1:-3258120 MsaT000244.1:CDS
CATGGGAGGAAGACATTTAC+TGG 0.353390 1:-3259481 MsaT000244.1:CDS
ATGTTTCTTCGGCATAAATC+TGG 0.355077 1:+3254862 None:intergenic
GCATAAATCTGGAAGTCTTA+TGG 0.355261 1:+3254873 None:intergenic
CCCCTAGTTCAGTTTGAGTA+TGG 0.362232 1:+3258229 None:intergenic
CCTTTAAGGGTCACATTGTC+TGG 0.362859 1:+3259827 None:intergenic
AGTTGTTGCTTAAAATCTTT+GGG 0.367729 1:+3259422 None:intergenic
ATAATCTTTATCTTGCAGTT+TGG 0.369783 1:+3255154 None:intergenic
GTTGTTTCCCCTTGAACATA+TGG 0.370462 1:+3254025 None:intergenic
ATCAATGAGGTTGGAAAAGA+TGG 0.373789 1:-3254959 MsaT000244.1:CDS
CACAAGTTGTTGTTGAAAAT+CGG 0.381409 1:+3261231 None:intergenic
CTGGCGAGAGAGCAATTGAT+TGG 0.381570 1:-3257681 MsaT000244.1:CDS
CGACATCAGCTAATTGTTGT+TGG 0.381642 1:-3257936 MsaT000244.1:CDS
CATTCAACACCGCCATTAGA+AGG 0.385293 1:+3259521 None:intergenic
TCCTTGTCTTTAAGTACAAT+GGG 0.391820 1:-3259694 MsaT000244.1:CDS
TGGGAAGAAGTTGGGTGAAA+TGG 0.393018 1:+3259441 None:intergenic
TAAAATCTTTGGGAAGAAGT+TGG 0.393785 1:+3259432 None:intergenic
AAAATCTTTGGGAAGAAGTT+GGG 0.404051 1:+3259433 None:intergenic
TACAGACATCTCCAATCTTT+AGG 0.410316 1:+3255020 None:intergenic
GTGGTGTTGCATGGAAAATA+GGG 0.415820 1:-3258437 MsaT000244.1:CDS
AACACCACCGGGACCTTTAA+GGG 0.419570 1:+3258452 None:intergenic
CAGGTGGAAGCAGCAAATCT+TGG 0.421511 1:-3261318 MsaT000244.1:CDS
TCCTCTGAGTTAAAGAAATC+CGG 0.424652 1:-3254761 MsaT000244.1:CDS
GATACCATTTACTTCACAAA+TGG 0.426840 1:-3259761 MsaT000244.1:CDS
CGAAGTATTTGAAGCTCTAC+CGG 0.428217 1:+3254742 None:intergenic
GTATCAGCTATGAACTGCTC+AGG 0.438283 1:+3258027 None:intergenic
TTTAACAAAATATGGAAAAG+AGG 0.438354 1:+3255938 None:intergenic
GTTTCAACAGGTACAGATAA+TGG 0.438533 1:+3258093 None:intergenic
TTCCTTGTCTTTAAGTACAA+TGG 0.443680 1:-3259695 MsaT000244.1:CDS
TGATCCTTCGAAAATTCATA+TGG 0.444278 1:+3255181 None:intergenic
GTTGTTGGAGAGCACTTGAC+TGG 0.446323 1:-3257921 MsaT000244.1:CDS
CCCCGCTTCTTACTCCGGCC+AGG 0.453081 1:+3257727 None:intergenic
TGATGCACGTTCGTATATCT+GGG 0.458399 1:-3253907 MsaT000244.1:CDS
GAAAAGAGGAGGTAAATGAT+TGG 0.467251 1:+3255952 None:intergenic
GAAGTCAATACTGCTTCTAA+TGG 0.478633 1:-3258171 MsaT000244.1:CDS
AACTCCACTAGGACCTTTAA+GGG 0.479312 1:+3259814 None:intergenic
GTCTTCCCCGCTTCTTACTC+CGG 0.480070 1:+3257722 None:intergenic
TTGTTTCCCCTTGAACATAT+GGG 0.482916 1:+3254026 None:intergenic
GCCTGGCCGGAGTAAGAAGC+GGG 0.483099 1:-3257728 MsaT000244.1:CDS
CGTTCGAAGATGGGAGGAGC+AGG 0.483535 1:-3261337 None:intergenic
CGTGTTCGCATCAATGAGGT+TGG 0.490884 1:-3254968 MsaT000244.1:CDS
AAGGTCTTGATCATCAAAAT+GGG 0.492524 1:-3253874 MsaT000244.1:CDS
AGGTGGAAGCAGCAAATCTT+GGG 0.493263 1:-3261317 MsaT000244.1:CDS
TTCCTTGTCTTTAATTACAA+TGG 0.493431 1:-3258333 MsaT000244.1:CDS
GGAGATGTGCAGTAGAGAAC+TGG 0.495082 1:+3254113 None:intergenic
TAGTATGCTTAGTGCAAGAC+AGG 0.498724 1:-3254906 MsaT000244.1:CDS
AAAAGATGGGTTAGACTCCC+AGG 0.502465 1:-3254945 MsaT000244.1:CDS
TCAAATACTTCGAAGAAGGC+AGG 0.504134 1:-3254731 MsaT000244.1:CDS
GATTATTCTTCATAATTTGA+AGG 0.504582 1:-3255137 MsaT000244.1:CDS
CGTGGCAATAGCCTAAAGAT+TGG 0.505082 1:-3255031 MsaT000244.1:CDS
AACTCCGTCCCATATGTTCA+AGG 0.509291 1:-3254034 MsaT000244.1:CDS
GAAGTCAGTACTCCTTCTAA+TGG 0.509411 1:-3259533 MsaT000244.1:CDS
AAAAGAGGAGGTAAATGATT+GGG 0.509472 1:+3255953 None:intergenic
GTGGAGTTGTGTGGAATGTC+GGG 0.511439 1:-3259799 MsaT000244.1:CDS
ATTATTCTTCATAATTTGAA+GGG 0.512712 1:-3255136 MsaT000244.1:CDS
GCAATTCAAGAGGCAAATGT+TGG 0.513221 1:-3254063 MsaT000244.1:CDS
GCCATACTCAAACTGAACTA+GGG 0.513331 1:-3258230 MsaT000244.1:CDS
GGCCATACTCAAACTGAACT+AGG 0.514000 1:-3258231 MsaT000244.1:CDS
CACGTTCGTATATCTGGGGT+TGG 0.517076 1:-3253902 MsaT000244.1:CDS
CAGTTTCCCTACCAACCAAC+CGG 0.519266 1:-3257982 MsaT000244.1:CDS
AGGAAACACCATGTTGATGA+TGG 0.520128 1:-3254394 MsaT000244.1:CDS
CCCCATTGTACTTAAAGACA+AGG 0.522025 1:+3259693 None:intergenic
AGTGGAGTTGTGTGGAATGT+CGG 0.523559 1:-3259800 MsaT000244.1:CDS
TCTGTTAATGAAGTTACGCC+TGG 0.524578 1:-3257745 MsaT000244.1:CDS
CTTCCCACTTCTATTCAGGA+TGG 0.529191 1:+3256046 None:intergenic
TGGCGAGAGAGCAATTGATT+GGG 0.530749 1:-3257680 MsaT000244.1:CDS
TGGAGATAGTTTCTGTGAGA+AGG 0.531697 1:-3259642 MsaT000244.1:CDS
GTAACTTCTGTATTGCAAGC+AGG 0.534058 1:+3254091 None:intergenic
GAAATGAATGAACTGAAAAT+GGG 0.535326 1:+3261276 None:intergenic
CTGTTTACTTCGTGGACGGT+TGG 0.535957 1:-3258395 MsaT000244.1:CDS
CACCATTGTAATTAAAGACA+AGG 0.542064 1:+3258331 None:intergenic
AGACGATACTGTTTACTTCG+TGG 0.544423 1:-3258403 MsaT000244.1:CDS
TATCAGCTATGAACTGCTCA+GGG 0.548226 1:+3258028 None:intergenic
TTAATGAAGTTACGCCTGGC+CGG 0.549314 1:-3257741 MsaT000244.1:CDS
AAGGTCCCGGTGGTGTTGCA+TGG 0.549921 1:-3258446 MsaT000244.1:CDS
AGTTCTAGGAACAGAATTGA+CGG 0.553971 1:+3255102 None:intergenic
TCGTATATCTGGGGTTGGAA+AGG 0.555220 1:-3253897 MsaT000244.1:CDS
TCTAATGGTGGTGTTGAATG+TGG 0.555737 1:-3258156 MsaT000244.1:CDS
CCATTTACTTCACAAATGGA+TGG 0.556295 1:-3259757 MsaT000244.1:CDS
AGCTTCAAATACTTCGAAGA+AGG 0.556522 1:-3254735 MsaT000244.1:CDS
AATAGAAGTGGGAAGGCAGA+TGG 0.559991 1:-3256038 MsaT000244.1:CDS
TTTCAAACTATCATTAGCCT+GGG 0.560665 1:+3254363 None:intergenic
ATATCTGGGGTTGGAAAGGA+AGG 0.562175 1:-3253893 MsaT000244.1:CDS
TGATTGGGTGGCTTGTAACA+AGG 0.569869 1:-3257665 MsaT000244.1:CDS
GAAATGGATGAACTGAAAAT+GGG 0.570637 1:+3259457 None:intergenic
GCTATCTCTTTCTCATCATG+TGG 0.571214 1:+3255975 None:intergenic
GTTACGCTTCGCAATTCAAG+AGG 0.575759 1:-3254073 MsaT000244.1:CDS
AATGTGACCCTTAAAGGTCC+CGG 0.577654 1:-3258459 MsaT000244.1:CDS
ATGAAGAAGTTCAATGTCTG+TGG 0.577831 1:-3254268 MsaT000244.1:CDS
AAGCTCCACTGAGAATATCA+TGG 0.579465 1:+3256015 None:intergenic
TACTGAACAAGGGTGTAACA+AGG 0.579623 1:-3259582 MsaT000244.1:CDS
GGACAAGCTCATACTGAACA+AGG 0.580719 1:-3259593 MsaT000244.1:CDS
TTGGAGAGCACTTGACTGGG+TGG 0.581287 1:-3257917 MsaT000244.1:CDS
CGAGAGAGCAATTGATTGGG+TGG 0.581381 1:-3257677 MsaT000244.1:CDS
ACATTCCACACAACTCCACT+AGG 0.582408 1:+3259803 None:intergenic
CAAATACTTCGAAGAAGGCA+GGG 0.582811 1:-3254730 MsaT000244.1:CDS
TCATCAACATGGTGTTTCCT+CGG 0.584118 1:+3254397 None:intergenic
AACAAAATATGGAAAAGAGG+AGG 0.585641 1:+3255941 None:intergenic
CGCCTGGCCGGAGTAAGAAG+CGG 0.586085 1:-3257729 MsaT000244.1:CDS
TTGTTGGAGAGCACTTGACT+GGG 0.586591 1:-3257920 MsaT000244.1:CDS
TTTCACAAAATATGGAAATG+GGG 0.587903 1:+3254294 None:intergenic
GTCACATTCTATGCGGAGGA+TGG 0.588116 1:-3255066 MsaT000244.1:CDS
CTCCGTCCCATATGTTCAAG+GGG 0.588313 1:-3254032 MsaT000244.1:CDS
TAACTTCTGTATTGCAAGCA+GGG 0.589388 1:+3254092 None:intergenic
TGTGCAGTAGAGAACTGGCA+TGG 0.592258 1:+3254118 None:intergenic
ACCGGATTTCTTTAACTCAG+AGG 0.593709 1:+3254760 None:intergenic
GTCAATACTGCTTCTAATGG+TGG 0.593959 1:-3258168 MsaT000244.1:CDS
GATGTCATTATCACGAACAA+AGG 0.594099 1:+3253973 None:intergenic
GCAGCCATCCTGAATAGAAG+TGG 0.595897 1:-3256050 MsaT000244.1:CDS
GATGGGTTAGACTCCCAGGT+TGG 0.599922 1:-3254941 MsaT000244.1:CDS
TTCCCCTTGAACATATGGGA+CGG 0.601024 1:+3254030 None:intergenic
TCGAAGATGGGAGGAGCAGG+TGG 0.601163 1:-3261334 None:intergenic
TTGTTGTTGAAAATCGGTGG+TGG 0.608194 1:+3261237 None:intergenic
GCTTATAACAAGCAACACTC+AGG 0.608918 1:-3257895 MsaT000244.1:intron
ACTCCGTCCCATATGTTCAA+GGG 0.617467 1:-3254033 MsaT000244.1:CDS
TTCTGATGTAGTCACTATAG+CGG 0.623932 1:-3257803 MsaT000244.1:intron
AAGGTCCTAGTGGAGTTGTG+TGG 0.624686 1:-3259808 MsaT000244.1:CDS
TGATGATGGACTAAGCTCCC+AGG 0.628271 1:-3254380 MsaT000244.1:CDS
CATCCTGAATAGAAGTGGGA+AGG 0.628564 1:-3256045 MsaT000244.1:CDS
GTGACCCTTAAAGGTCCTAG+TGG 0.630968 1:-3259818 MsaT000244.1:CDS
GAACAACTCACCAGAATACG+TGG 0.631909 1:+3255888 None:intergenic
GTCTTGCGTTCGAAGATGGG+AGG 0.632969 1:-3261343 None:intergenic
CCATACTCAAACTGAACTAG+GGG 0.634393 1:-3258229 MsaT000244.1:CDS
ATTACGTGTTCGCATCAATG+AGG 0.634490 1:-3254972 MsaT000244.1:CDS
GTCAGTACTCCTTCTAATGG+CGG 0.637844 1:-3259530 MsaT000244.1:CDS
GCTTGTAACAAGGAAAACTC+AGG 0.638956 1:-3257655 MsaT000244.1:intron
GATACTGTTTACTTCGTGGA+CGG 0.642050 1:-3258399 MsaT000244.1:CDS
CAGCCATCCTGAATAGAAGT+GGG 0.642084 1:-3256049 MsaT000244.1:CDS
TCAATGAGGTTGGAAAAGAT+GGG 0.644296 1:-3254958 MsaT000244.1:CDS
AGCTTAGTCCATCATCAACA+TGG 0.648752 1:+3254386 None:intergenic
GCAAGTCACATTCTATGCGG+AGG 0.649025 1:-3255070 MsaT000244.1:CDS
GTAGGGAAACTGTATAGCTG+TGG 0.649466 1:+3257993 None:intergenic
ACTGAACAAGGGTGTAACAA+GGG 0.652043 1:-3259581 MsaT000244.1:CDS
ATAATTTGAAGGGAGAACAG+TGG 0.652096 1:-3255126 MsaT000244.1:CDS
GTGCAGTAGAGAACTGGCAT+GGG 0.660376 1:+3254119 None:intergenic
CCTTGTCTTTAAGTACAATG+GGG 0.669263 1:-3259693 MsaT000244.1:CDS
AAGTTGTTGTTGAAAATCGG+TGG 0.686571 1:+3261234 None:intergenic
GATGGCCATGATATTCTCAG+TGG 0.691057 1:-3256020 MsaT000244.1:CDS
GACAAGCTCATACTGAACAA+GGG 0.699080 1:-3259592 MsaT000244.1:CDS
TGTCTGTGGATCATACACTT+TGG 0.709889 1:-3254254 MsaT000244.1:intron
GTGACCCTTAAAGGTCCCGG+TGG 0.715661 1:-3258456 MsaT000244.1:CDS
CCTGGCCGGAGTAAGAAGCG+GGG 0.719494 1:-3257727 MsaT000244.1:CDS
AAACAAAGACAGCTGTACCG+AGG 0.720792 1:-3254414 MsaT000244.1:intron
GATGCACGTTCGTATATCTG+GGG 0.734162 1:-3253906 MsaT000244.1:CDS
CATGCAAGTCACATTCTATG+CGG 0.736069 1:-3255073 MsaT000244.1:CDS
ATCAGCTATGAACTGCTCAG+GGG 0.739451 1:+3258029 None:intergenic
AATCAGCTCAAAGTGCAGTG+CGG 0.745608 1:-3254837 MsaT000244.1:CDS
CATACTCAAACTGAACTAGG+GGG 0.777970 1:-3258228 MsaT000244.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 3253859 3261350 3253859 ID=MsaG000244
Chr1 mRNA 3253859 3261350 3253859 ID=MsaT000244.1;Parent=MsaG000244
Chr1 exon 3253859 3254146 3253859 ID=MsaT000244.1.exon8;Parent=MsaT000244.1
Chr1 CDS 3253859 3254146 3253859 ID=cds.MsaT000244.1;Parent=MsaT000244.1
Chr1 exon 3254255 3254424 3254255 ID=MsaT000244.1.exon7;Parent=MsaT000244.1
Chr1 CDS 3254255 3254424 3254255 ID=cds.MsaT000244.1;Parent=MsaT000244.1
Chr1 exon 3254693 3255209 3254693 ID=MsaT000244.1.exon6;Parent=MsaT000244.1
Chr1 CDS 3254693 3255209 3254693 ID=cds.MsaT000244.1;Parent=MsaT000244.1
Chr1 exon 3255899 3256074 3255899 ID=MsaT000244.1.exon5;Parent=MsaT000244.1
Chr1 CDS 3255899 3256074 3255899 ID=cds.MsaT000244.1;Parent=MsaT000244.1
Chr1 exon 3257656 3257814 3257656 ID=MsaT000244.1.exon4;Parent=MsaT000244.1
Chr1 CDS 3257656 3257814 3257656 ID=cds.MsaT000244.1;Parent=MsaT000244.1
Chr1 exon 3257896 3258529 3257896 ID=MsaT000244.1.exon3;Parent=MsaT000244.1
Chr1 CDS 3257896 3258529 3257896 ID=cds.MsaT000244.1;Parent=MsaT000244.1
Chr1 exon 3259421 3259891 3259421 ID=MsaT000244.1.exon2;Parent=MsaT000244.1
Chr1 CDS 3259421 3259891 3259421 ID=cds.MsaT000244.1;Parent=MsaT000244.1
Chr1 exon 3261234 3261350 3261234 ID=MsaT000244.1.exon1;Parent=MsaT000244.1
Chr1 CDS 3261234 3261350 3261234 ID=cds.MsaT000244.1;Parent=MsaT000244.1
Gene Sequence

>MsaT000244.1

ATGGGAGGAGCAGGTGGAAGCAGCAAATCTTGGGAGAAAGAGATTTACTGGACCCATTTTCAGTTCATTCATTTCATCCAATTTCTTCAAACCACCACCGATTTTCAACAACAACTTGCCCTTCCCAAAACATTTTCAGACAATTTAAAGAAGAAGTTGCCAGACAATGTGACCCTTAAAGGTCCTAGTGGAGTTGTGTGGAATGTCGGGCTGACAGCTAGAGATGATACCATTTACTTCACAAATGGATGGCAAAGATTCGTGAAAGATCACTCTTTGAAAGAGAATGATTTCCTTGTCTTTAAGTACAATGGGGAATCACTCTTTGAAGTTTTAATCTTTCATGGAGATAGTTTCTGTGAGAAGGCAGCTTCTTATTTTGTTGGAAAATGTGGACAAGCTCATACTGAACAAGGGTGTAACAAGGGAAAAAGCTCCAATAATTCTGTTGAAGAAGTCAGTACTCCTTCTAATGGCGGTGTTGAATGCAGTTCGCCTGAAAAATCATGGGAGGAAGACATTTACTGGACCCATTTTCAGTTCATCCATTTCACCCAACTTCTTCCCAAAGATTTTAAGCAACAACTTGCACTTCCTAAAACATTTTCAGACAATTTAAATAAGAAGTTGCCAGAAAATGTGACCCTTAAAGGTCCCGGTGGTGTTGCATGGAAAATAGGGCTCATAACTAGAGACGATACTGTTTACTTCGTGGACGGTTGGCAACGATTCGTGAACGATCACTCTTTGAAAGATAATGATTTCCTTGTCTTTAATTACAATGGTGAATCACACTTTGATGTTTTGATCTTTGATGGAGAAAGCTTCTGTGAGAAAGAAGCTTCTTATTTTGTCGGAAAATGCGGCCATACTCAAACTGAACTAGGGGGTAGCAAAGCAAAGGAAACAAACAATTCTATTGAAGAAGTCAATACTGCTTCTAATGGTGGTGTTGAATGTGGTTTATATGAGAAATTTCAGGATCTTAACAATATAGGAACACCATTATCTGTACCTGTTGAAACTACCAATGAAAAGACTTTTAATGCTGGTGTCAAATCTGATTCCCCTGAGCAGTTCATAGCTGATACAGTCACTAAGACCACAGCTATACAGTTTCCCTACCAACCAACCGGAAAAAGGAGCAAGAGAAGATCATCGACATCAGCTAATTGTTGTTGGAGAGCACTTGACTGGGTGGCTTATAACAAGCAACACTCAGTCACTATAGCGGTTCCCTCCCATACAACCAACCAAAAGACCAAGAAGTCTGTTAATGAAGTTACGCCTGGCCGGAGTAAGAAGCGGGGAAGACAAGCAAAAACAGCTACTTCTGGCGAGAGAGCAATTGATTGGGTGGCTTGTAACAAGGAAAACTCAGAGGCAGCCATCCTGAATAGAAGTGGGAAGGCAGATGGCCATGATATTCTCAGTGGAGCTTCACTGTCTAAGTTATCACCACATGATGAGAAAGAGATAGCCCAATCATTTACCTCCTCTTTTCCATATTTTGTTAAAATAATGAAAACGTTCAATGTTGAACGTCCACGTATTCTGAATATTCCATATGAATTTTCGAAGGATCATCTTCCAAACTGCAAGATAAAGATTATTCTTCATAATTTGAAGGGAGAACAGTGGACCGTCAATTCTGTTCCTAGAACTAGATTGCATGCAAGTCACATTCTATGCGGAGGATGGTTGAATTTTGTTCGTGGCAATAGCCTAAAGATTGGAGATGTCTGTATTTTCGAACTTATAGGTGAGTGTGAATTACGTGTTCGCATCAATGAGGTTGGAAAAGATGGGTTAGACTCCCAGGTTGGAAAACTAGCTTGTAGTATGCTTAGTGCAAGACAGGATGTCGCTAGCCATAAGACTTCCAGATTTATGCCGAAGAAACATAAAATCAGCTCAAAGTGCAGTGCGGAAGTTGATCTATTAGATAAAAAGTTGTCAGAGATATGTCAAGAAGCTGCTTTATCCTCTGAGTTAAAGAAATCCGGTAGAGCTTCAAATACTTCGAAGAAGGCAGGGCTTTCCCCCAAGTCAAAAGCTGCCCATAAAAAGTCGGCTGTACCGAGGAAACACCATGTTGATGATGGACTAAGCTCCCAGGCTAATGATAGTTTGAAAATGTTGTTTGCACTCGACGAACAAAGAGTAGCTGAAGCTTTCACTTCCCCATTTCCATATTTTGTGAAAATCATGAAGAAGTTCAATGTCTGTGGATCATACACTTTGAAAATCCCATGCCAGTTCTCTACTGCACATCTCCCTGCTTGCAATACAGAAGTTACGCTTCGCAATTCAAGAGGCAAATGTTGGACTGTGAACTCCGTCCCATATGTTCAAGGGGAAACAACTATTCACACTTTTTGTGGCGGATGGAATGCCTTTGTTCGTGATAATGACATCAATTTTGGTGACACTTGTATTTTTGAGCTCGTTTCTGATTATGTGATGCACGTTCGTATATCTGGGGTTGGAAAGGAAGGTCTTGATCATCAAAATGGGAATGTGAAACAATAG
Protein sequence

>MsaT000244.1

MGGAGGSSKSWEKEIYWTHFQFIHFIQFLQTTTDFQQQLALPKTFSDNLKKKLPDNVTLKGPSGVVWNVGLTARDDTIYFTNGWQRFVKDHSLKENDFLVFKYNGESLFEVLIFHGDSFCEKAASYFVGKCGQAHTEQGCNKGKSSNNSVEEVSTPSNGGVECSSPEKSWEEDIYWTHFQFIHFTQLLPKDFKQQLALPKTFSDNLNKKLPENVTLKGPGGVAWKIGLITRDDTVYFVDGWQRFVNDHSLKDNDFLVFNYNGESHFDVLIFDGESFCEKEASYFVGKCGHTQTELGGSKAKETNNSIEEVNTASNGGVECGLYEKFQDLNNIGTPLSVPVETTNEKTFNAGVKSDSPEQFIADTVTKTTAIQFPYQPTGKRSKRRSSTSANCCWRALDWVAYNKQHSVTIAVPSHTTNQKTKKSVNEVTPGRSKKRGRQAKTATSGERAIDWVACNKENSEAAILNRSGKADGHDILSGASLSKLSPHDEKEIAQSFTSSFPYFVKIMKTFNVERPRILNIPYEFSKDHLPNCKIKIILHNLKGEQWTVNSVPRTRLHASHILCGGWLNFVRGNSLKIGDVCIFELIGECELRVRINEVGKDGLDSQVGKLACSMLSARQDVASHKTSRFMPKKHKISSKCSAEVDLLDKKLSEICQEAALSSELKKSGRASNTSKKAGLSPKSKAAHKKSAVPRKHHVDDGLSSQANDSLKMLFALDEQRVAEAFTSPFPYFVKIMKKFNVCGSYTLKIPCQFSTAHLPACNTEVTLRNSRGKCWTVNSVPYVQGETTIHTFCGGWNAFVRDNDINFGDTCIFELVSDYVMHVRISGVGKEGLDHQNGNVKQ*