AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG000319

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000319 MtrunA17_Chr6g0481091 32.203 177 89 8 4 166 6 165 1.92e-17 79.0
MsaG000319 MtrunA17_Chr4g0056541 30.719 153 89 5 5 146 8 154 1.33e-14 72.4
MsaG000319 MtrunA17_Chr3g0130731 31.056 161 83 8 4 147 7 156 1.72e-14 71.6
MsaG000319 MtrunA17_Chr2g0318101 32.530 166 87 7 4 161 15 163 1.88e-14 72.0
MsaG000319 MtrunA17_Chr8g0384721 31.056 161 84 7 4 147 7 157 1.89e-14 71.2
MsaG000319 MtrunA17_Chr2g0326361 32.075 159 84 5 4 145 14 165 2.28e-14 71.2
MsaG000319 MtrunA17_Chr4g0050701 26.178 191 116 7 4 180 11 190 9.44e-14 67.8
MsaG000319 MtrunA17_Chr7g0263931 32.716 162 79 7 4 145 11 162 1.22e-13 69.3
MsaG000319 MtrunA17_Chr3g0130101 28.571 210 125 8 2 195 5 205 1.24e-13 69.7
MsaG000319 MtrunA17_Chr4g0076731 33.553 152 82 7 6 146 56 199 2.07e-13 69.3
MsaG000319 MtrunA17_Chr4g0017921 32.895 152 86 7 5 146 9 154 2.43e-13 68.6
MsaG000319 MtrunA17_Chr5g0428991 31.875 160 81 9 4 146 25 173 4.17e-13 68.6
MsaG000319 MtrunA17_Chr8g0345671 32.237 152 84 6 5 145 12 155 4.33e-13 68.2
MsaG000319 MtrunA17_Chr8g0361731 28.743 167 90 6 2 146 12 171 4.36e-13 68.2
MsaG000319 MtrunA17_Chr8g0384351 30.058 173 98 8 4 173 10 162 6.65e-13 65.5
MsaG000319 MtrunA17_Chr3g0144271 30.405 148 91 5 4 145 23 164 9.52e-13 67.0
MsaG000319 MtrunA17_Chr7g0261411 30.769 156 84 8 4 146 10 154 1.02e-12 66.2
MsaG000319 MtrunA17_Chr4g0054791 31.875 160 84 9 4 146 6 157 1.06e-12 66.6
MsaG000319 MtrunA17_Chr6g0484441 30.769 156 88 7 2 146 3 149 1.17e-12 66.6
MsaG000319 MtrunA17_Chr4g0038751 30.000 150 89 6 6 146 17 159 2.99e-12 65.1
MsaG000319 MtrunA17_Chr1g0201021 33.333 135 73 6 6 132 21 146 7.46e-12 64.7
MsaG000319 MtrunA17_Chr2g0318081 29.936 157 86 7 4 146 16 162 8.91e-12 63.9
MsaG000319 MtrunA17_Chr1g0197351 31.677 161 82 7 6 146 44 196 1.12e-11 63.9
MsaG000319 MtrunA17_Chr2g0309891 28.931 159 88 8 4 146 6 155 1.24e-11 63.5
MsaG000319 MtrunA17_Chr4g0060931 26.733 202 120 8 4 190 17 205 3.26e-11 62.4
MsaG000319 MtrunA17_Chr8g0359921 28.931 159 87 7 4 146 14 162 3.71e-11 62.0
MsaG000319 MtrunA17_Chr8g0385991 31.429 140 75 6 4 134 14 141 3.89e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000319 AT2G24430.2 35.032 157 83 6 2 146 14 163 6.64e-18 81.6
MsaG000319 AT2G24430.1 35.032 157 83 6 2 146 14 163 6.64e-18 81.6
MsaG000319 AT1G71930.2 32.484 157 89 5 4 149 9 159 2.53e-17 80.1
MsaG000319 AT1G71930.1 32.484 157 89 5 4 149 9 159 2.53e-17 80.1
MsaG000319 AT2G27300.1 29.861 144 91 4 6 145 16 153 1.36e-16 78.2
MsaG000319 AT3G17730.1 31.847 157 85 7 4 146 6 154 2.50e-16 76.3
MsaG000319 AT3G10490.1 32.692 156 82 7 6 146 29 176 6.68e-16 75.1
MsaG000319 AT3G49530.2 28.571 161 92 7 1 146 10 162 2.63e-15 75.1
MsaG000319 AT3G49530.1 28.571 161 92 7 1 146 10 162 2.70e-15 75.1
MsaG000319 AT3G10490.2 32.692 156 82 7 6 146 29 176 3.60e-15 74.7
MsaG000319 AT1G26870.1 34.000 150 86 4 6 149 18 160 3.92e-15 74.7
MsaG000319 AT5G08790.1 31.902 163 81 8 4 147 7 158 7.60e-15 72.8
MsaG000319 AT3G10480.1 32.692 156 82 7 6 146 29 176 1.08e-14 73.6
MsaG000319 AT1G77450.1 31.875 160 83 6 4 147 10 159 1.62e-14 71.2
MsaG000319 AT5G46590.1 33.333 159 80 8 4 145 6 155 3.63e-14 70.9
MsaG000319 AT2G02450.1 34.194 155 83 7 6 149 53 199 3.87e-14 71.6
MsaG000319 AT3G10480.2 32.258 155 83 7 6 146 29 175 4.12e-14 71.6
MsaG000319 AT2G02450.2 34.194 155 83 7 6 149 53 199 4.52e-14 71.6
MsaG000319 AT4G27410.2 29.630 162 90 7 4 151 14 165 1.20e-13 69.3
MsaG000319 AT1G33280.1 32.237 152 87 5 5 146 9 154 1.22e-13 69.7
MsaG000319 AT1G54330.1 31.613 155 85 7 4 146 6 151 1.24e-13 69.3
MsaG000319 AT5G64530.1 29.091 165 83 6 4 147 3 154 2.87e-13 66.6
MsaG000319 AT4G28530.1 28.313 166 93 5 4 147 10 171 2.99e-13 68.9
MsaG000319 AT5G46590.2 34.286 140 74 7 4 134 6 136 6.92e-13 67.0
MsaG000319 AT5G39610.1 31.013 158 85 7 4 146 20 168 8.70e-13 67.0
MsaG000319 AT5G64530.2 32.061 131 76 4 4 134 3 120 9.56e-13 64.7
MsaG000319 AT3G15500.1 29.487 156 88 7 4 146 14 160 2.50e-12 65.9
MsaG000319 AT3G18400.1 30.380 158 89 8 2 147 3 151 2.68e-12 65.9
MsaG000319 AT5G04410.1 29.221 154 89 6 6 146 11 157 2.73e-12 66.6
MsaG000319 AT5G61430.1 29.448 163 91 6 4 151 16 169 7.01e-12 64.7
MsaG000319 AT2G17040.1 29.333 150 90 6 6 146 8 150 1.21e-11 63.5
MsaG000319 AT1G52890.1 28.662 157 88 7 4 146 14 160 1.46e-11 63.5
MsaG000319 AT3G10500.1 31.410 156 83 7 6 146 11 157 2.18e-11 63.9
MsaG000319 AT3G29035.1 29.747 158 87 6 4 146 24 172 3.24e-11 62.8

Find 56 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAGCTTAAGCATGCACTTC+TGG 0.248003 1:+4270863 MsaT000319.1:CDS
CAATGGTTCTGAGCTAATTA+AGG 0.256935 1:+4269448 MsaT000319.1:CDS
GGTACTGTAAGACAGGATTT+TGG 0.279481 1:+4269582 MsaT000319.1:CDS
GGAGCTGATAACGTGGTTCT+AGG 0.360842 1:+4269638 MsaT000319.1:CDS
CCACCAGGGATGGATCTAAA+AGG 0.365878 1:+4269901 MsaT000319.1:CDS
GCTGGTGCTAGTGCTGCTAT+TGG 0.369554 1:+4270003 MsaT000319.1:CDS
CGTGTGTTTGACTGCCCACC+AGG 0.419940 1:+4269886 MsaT000319.1:CDS
GAAGAGATTCAGAATTTCAA+TGG 0.424440 1:+4269431 MsaT000319.1:CDS
AGGTGATGCATCGTATTTGC+AGG 0.428163 1:+4270065 MsaT000319.1:CDS
GGAGTAAGACGCAATTCAAA+TGG 0.431789 1:-4269494 None:intergenic
TGATGTCGCTCAGAAACCTA+TGG 0.450742 1:+4269978 MsaT000319.1:CDS
GCAATTCAAATGGGTCATAC+TGG 0.459319 1:-4269484 None:intergenic
GCTAGTGCTGCTATTGGTGT+TGG 0.469075 1:+4270009 MsaT000319.1:CDS
AACACACGACAAACAACAAA+AGG 0.472249 1:-4269871 None:intergenic
CAGAGGAGGTACTGTAAGAC+AGG 0.478625 1:+4269575 MsaT000319.1:CDS
CCTCCTCTGCTCATCAACTT+TGG 0.480636 1:-4269561 None:intergenic
GTGAAGCAATAAAATTCCCT+TGG 0.488088 1:-4269536 None:intergenic
CAAATGGGTCATACTGGTAC+AGG 0.492656 1:-4269478 None:intergenic
ATGGATCTAAAAGGTGAGTT+CGG 0.503657 1:+4269910 MsaT000319.1:CDS
CCTTGGGTGACGGAAGGAAA+AGG 0.509656 1:-4269519 None:intergenic
GAAGGGACGAAGCCAACGCC+AGG 0.516186 1:-4269365 None:intergenic
GATGTCGCTCAGAAACCTAT+GGG 0.517207 1:+4269979 MsaT000319.1:CDS
GTTGGTTCTACTGCTGATGC+TGG 0.518251 1:+4270027 MsaT000319.1:CDS
GAATTGTTTCCACCTGGCGT+TGG 0.519445 1:+4269353 MsaT000319.1:CDS
AGTGCATGCTTAAGCTGCTC+CGG 0.526169 1:-4270858 None:intergenic
AATAAAATTCCCTTGGGTGA+CGG 0.537471 1:-4269529 None:intergenic
CACTCTTGCTAACAAGCCTC+AGG 0.544263 1:+4269739 MsaT000319.1:CDS
GTTGAAGAAGGCGATGCTGC+TGG 0.544550 1:+4269946 MsaT000319.1:CDS
TGGACGCTGTCAAAACCAGC+TGG 0.550147 1:+4269702 MsaT000319.1:CDS
TGAAGCAATAAAATTCCCTT+GGG 0.552712 1:-4269535 None:intergenic
CCACATGGAAGCTATGACAC+CGG 0.554580 1:+4270839 MsaT000319.1:CDS
GCTGGTCGTGCTGCTGAGCA+AGG 0.554733 1:+4270045 MsaT000319.1:CDS
GAGTAAGACGCAATTCAAAT+GGG 0.556312 1:-4269493 None:intergenic
AGGTGAGTTCGGAGCCTTGA+CGG 0.561338 1:+4269921 MsaT000319.1:CDS
GCCTTGACGGATGTTGAAGA+AGG 0.563038 1:+4269934 MsaT000319.1:CDS
GTTTGACTGCCCACCAGGGA+TGG 0.566884 1:+4269891 MsaT000319.1:CDS
GCTGTTCGAGACATTACCGT+TGG 0.568138 1:+4269617 MsaT000319.1:CDS
GCAATGGAATTGTTTCCACC+TGG 0.585685 1:+4269347 None:intergenic
AAATTCCCTTGGGTGACGGA+AGG 0.596649 1:-4269525 None:intergenic
GCCTTCTTCAACATCCGTCA+AGG 0.599602 1:-4269935 None:intergenic
GTACTCGTACATAGTCCAGC+TGG 0.603542 1:-4269717 None:intergenic
CTAACAAGGAGCTCATCAGA+AGG 0.607644 1:-4269383 None:intergenic
TAACAAGGAGCTCATCAGAA+GGG 0.608778 1:-4269382 None:intergenic
CCAAAGTTGATGAGCAGAGG+AGG 0.609229 1:+4269561 MsaT000319.1:CDS
TGACTGTGTTGTCTTCCACA+TGG 0.610846 1:+4270824 MsaT000319.1:CDS
CTATTTAGGAAATTCACCGA+TGG 0.613156 1:+4269682 MsaT000319.1:CDS
TGGCAAGATAGTAGCTAACA+AGG 0.620882 1:-4269397 None:intergenic
GGGACGAAGCCAACGCCAGG+TGG 0.622803 1:-4269362 None:intergenic
CTGCCAAAGTTGATGAGCAG+AGG 0.624165 1:+4269558 MsaT000319.1:CDS
AACCACGTTATCAGCTCCAA+CGG 0.626095 1:-4269633 None:intergenic
GGGTGACGGAAGGAAAAGGA+TGG 0.637555 1:-4269515 None:intergenic
TACCGTTGGAGCTGATAACG+TGG 0.651576 1:+4269631 MsaT000319.1:CDS
ATGTCGCTCAGAAACCTATG+GGG 0.679776 1:+4269980 MsaT000319.1:CDS
CCGGTGTCATAGCTTCCATG+TGG 0.695251 1:-4270839 None:intergenic
GTGTGTTTGACTGCCCACCA+GGG 0.714218 1:+4269887 MsaT000319.1:CDS
TGTCGCTCAGAAACCTATGG+GGG 0.722313 1:+4269981 MsaT000319.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 4269350 4270920 4269350 ID=MsaG000319
Chr1 mRNA 4269350 4270920 4269350 ID=MsaT000319.1;Parent=MsaG000319
Chr1 exon 4269350 4269760 4269350 ID=MsaT000319.1.exon1;Parent=MsaT000319.1
Chr1 CDS 4269350 4269760 4269350 ID=cds.MsaT000319.1;Parent=MsaT000319.1
Chr1 exon 4269868 4270086 4269868 ID=MsaT000319.1.exon2;Parent=MsaT000319.1
Chr1 CDS 4269868 4270086 4269868 ID=cds.MsaT000319.1;Parent=MsaT000319.1
Chr1 exon 4270804 4270920 4270804 ID=MsaT000319.1.exon3;Parent=MsaT000319.1
Chr1 CDS 4270804 4270920 4270804 ID=cds.MsaT000319.1;Parent=MsaT000319.1
Gene Sequence

>MsaT000319.1

ATGGAATTGTTTCCACCTGGCGTTGGCTTCGTCCCTTCTGATGAGCTCCTTGTTAGCTACTATCTTGCCAACAAAAACAATGAAGAGATTCAGAATTTCAATGGTTCTGAGCTAATTAAGGAGCTGAACCTGTACCAGTATGACCCATTTGAATTGCGTCTTACTCCATCCTTTTCCTTCCGTCACCCAAGGGAATTTTATTGCTTCACTGCCAAAGTTGATGAGCAGAGGAGGTACTGTAAGACAGGATTTTGGATGCAAAAGGGAGCTGTTCGAGACATTACCGTTGGAGCTGATAACGTGGTTCTAGGAAAGAAATCTCTCTTCTTTTTCTATTTAGGAAATTCACCGATGGACGCTGTCAAAACCAGCTGGACTATGTACGAGTACACTCTTGCTAACAAGCCTCAGGCTCCTTTTGTTGTTTGTCGTGTGTTTGACTGCCCACCAGGGATGGATCTAAAAGGTGAGTTCGGAGCCTTGACGGATGTTGAAGAAGGCGATGCTGCTGGCGATGACTCTGATGTCGCTCAGAAACCTATGGGGGCTGGTGCTAGTGCTGCTATTGGTGTTGGTTCTACTGCTGATGCTGGTCGTGCTGCTGAGCAAGGTGATGCATCGTATTTGCAGGCGAGTTATGCTGCATTTCTTGACTGTGTTGTCTTCCACATGGAAGCTATGACACCGGAGCAGCTTAAGCATGCACTTCTGGACGAAGATTTTCTTGAACTCGCTGATTTTGTATAA
Protein sequence

>MsaT000319.1

MELFPPGVGFVPSDELLVSYYLANKNNEEIQNFNGSELIKELNLYQYDPFELRLTPSFSFRHPREFYCFTAKVDEQRRYCKTGFWMQKGAVRDITVGADNVVLGKKSLFFFYLGNSPMDAVKTSWTMYEYTLANKPQAPFVVCRVFDCPPGMDLKGEFGALTDVEEGDAAGDDSDVAQKPMGAGASAAIGVGSTADAGRAAEQGDASYLQASYAAFLDCVVFHMEAMTPEQLKHALLDEDFLELADFV*