AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG000321


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 31 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACCGGATTCTGGAGGAAAA+AGG 0.330121 1:-4292333 MsaT000321.1:CDS
GACACTGCAAGACCGGATTC+TGG 0.388814 1:-4292343 MsaT000321.1:CDS
ATGGATCTGGATGCTGAGTT+TGG 0.391512 1:-4292020 MsaT000321.1:CDS
TCAGCATCCAGATCCATCTT+TGG 0.442178 1:+4292026 None:intergenic
GTTAGTTCTGATAGCAGTGT+TGG 0.458231 1:+4292229 None:intergenic
TTAGTTCTGATAGCAGTGTT+GGG 0.461166 1:+4292230 None:intergenic
CTGCCCACCAAAGATGGATC+TGG 0.482322 1:-4292033 MsaT000321.1:CDS
ACACTGCTATCAGAACTAAC+TGG 0.486063 1:-4292226 MsaT000321.1:CDS
TGGAGTTACTCAGAAACCTA+TGG 0.490884 1:-4291937 MsaT000321.1:CDS
AAAACACGACAAATGACAAA+AGG 0.501966 1:+4292059 None:intergenic
GAGAGAAGACACTGCAAGAC+CGG 0.507192 1:-4292350 MsaT000321.1:CDS
GATAATGTATGAGCATGCTT+TGG 0.511387 1:-4292204 MsaT000321.1:CDS
GACTCTGCTGACAAGATGAA+TGG 0.517567 1:-4291957 MsaT000321.1:CDS
TGGTGCTAGTAATGTTGTGT+TGG 0.520269 1:-4292291 MsaT000321.1:CDS
TGCTTTGGCTGACAAGCCTC+AGG 0.528648 1:-4292189 MsaT000321.1:intron
GCATCCAGATCCATCTTTGG+TGG 0.546474 1:+4292029 None:intergenic
ACCATCACCTTCATCAACCT+TGG 0.550147 1:+4291989 None:intergenic
GCGTTATCCAAGGTTGATGA+AGG 0.556534 1:-4291996 MsaT000321.1:CDS
GGTGCTAGTAATGTTGTGTT+GGG 0.557003 1:-4292290 MsaT000321.1:CDS
GGAGTTACTCAGAAACCTAT+GGG 0.559803 1:-4291936 MsaT000321.1:CDS
ACTAGTGTTGCTGCTGAGCA+AGG 0.563406 1:-4291894 MsaT000321.1:CDS
TAGTTCTGATAGCAGTGTTG+GGG 0.568976 1:+4292231 None:intergenic
CATCCAGATCCATCTTTGGT+GGG 0.591585 1:+4292030 None:intergenic
GGAGCTGTTCAAGACATCAC+TGG 0.599122 1:-4292311 MsaT000321.1:CDS
TGAGTTTGGAGCGTTATCCA+AGG 0.615415 1:-4292006 MsaT000321.1:CDS
AGTTCTGATAGCAGTGTTGG+GGG 0.663335 1:+4292232 None:intergenic
TCCAAGGTTGATGAAGGTGA+TGG 0.681651 1:-4291990 MsaT000321.1:CDS
ACTGCAAGACCGGATTCTGG+AGG 0.686245 1:-4292340 MsaT000321.1:CDS
GAGTTACTCAGAAACCTATG+GGG 0.687052 1:-4291935 MsaT000321.1:CDS
AGTGTTGCTGCTGAGCAAGG+CGG 0.699580 1:-4291891 MsaT000321.1:CDS
AGTTACTCAGAAACCTATGG+GGG 0.734801 1:-4291934 MsaT000321.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 4291321 4292432 4291321 ID=MsaG000321
Chr1 mRNA 4291321 4292432 4291321 ID=MsaT000321.1;Parent=MsaG000321
Chr1 exon 4291321 4291350 4291321 ID=MsaT000321.1.exon3;Parent=MsaT000321.1
Chr1 CDS 4291321 4291350 4291321 ID=cds.MsaT000321.1;Parent=MsaT000321.1
Chr1 exon 4291854 4292084 4291854 ID=MsaT000321.1.exon2;Parent=MsaT000321.1
Chr1 CDS 4291854 4292084 4291854 ID=cds.MsaT000321.1;Parent=MsaT000321.1
Chr1 exon 4292190 4292432 4292190 ID=MsaT000321.1.exon1;Parent=MsaT000321.1
Chr1 CDS 4292190 4292432 4292190 ID=cds.MsaT000321.1;Parent=MsaT000321.1
Gene Sequence

>MsaT000321.1

ATGAAGGATGTTCATACTCAATCTCAATGCCAAACGCAAAATTATCTCTTTCAACACATTGAGAGAAGACACTGCAAGACCGGATTCTGGAGGAAAAAGGGAGCTGTTCAAGACATCACTGGTGCTAGTAATGTTGTGTTGGGCAAGAAGACCCTGTTTATTTTTTATCTTGCAAGTTCCCCCAACACTGCTATCAGAACTAACTGGATAATGTATGAGCATGCTTTGGCTGACAAGCCTCAGGCTCCTTTTGTCATTTGTCGTGTTTTTGACTGCCCACCAAAGATGGATCTGGATGCTGAGTTTGGAGCGTTATCCAAGGTTGATGAAGGTGATGGTGCTAGAGATGACTCTGCTGACAAGATGAATGGAGTTACTCAGAAACCTATGGGGGCTGGGGATGGGGTTGGTACTAGTGTTGCTGCTGAGCAAGGCGGTGCTTCAAATTTCACAGATTTTGAAGGCACCCTGCAGCAGTTTATAATTTTCTCGTCTTTGAAGTAG

Protein sequence

>MsaT000321.1

MKDVHTQSQCQTQNYLFQHIERRHCKTGFWRKKGAVQDITGASNVVLGKKTLFIFYLASSPNTAIRTNWIMYEHALADKPQAPFVICRVFDCPPKMDLDAEFGALSKVDEGDGARDDSADKMNGVTQKPMGAGDGVGTSVAAEQGGASNFTDFEGTLQQFIIFSSLK*